Thomas Böldicke

Thomas Böldicke received his PhD 1982 at the Max-Planck-Institut of Molecular Genetics, Berlin. He started his carrier as post doc at the German Research Centre for Biotechnology (GBF, Brunswick) in the Department of Genetics and Cell Biology by John Collins. Now he is senior scientist at the Helmholtz Centre for Infection Research (HZI, former GBF) and project leader intrabodies. 2011 he qualified as a professor in molecular biology and cell biology at the Technical University of Braunschweig, Germany. He is an expert in generating mouse and human hybridomas and in selecting and modifying recombinant antibodies. In the last decade he focused on the construction and characterization of intracellular antibodies. He has published 35 manuscripts.

1books edited

1chapters authored

Latest work with IntechOpen by Thomas Böldicke

Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets.

Go to the book