Hemoglobin release test (HRT), which is established by our lab, is a new experiment to observe the re-released hemoglobin (Hb) from erythrocytes. In this study, one-dimension HRT, double dimension HRT, and isotonic and hypotonic HRT were performed to observe the re-released Hb from the blood samples of normal adult, hereditary spherocytosis (HS), and α-thalassemia. The results showed that compared with normal adult, the re-released Hb from HS blood sample was decreased significantly; however, the re-released Hb from α-thalassemia blood sample was increased significantly. The mechanism of this phenomenon was speculated to have relation with the abnormal amount of membrane-binding Hb.
Part of the book: Inherited Hemoglobin Disorders