Chapters authored
Sperm DNA Fragmentation and Its Relation With Fertility
Sperm DNA integrity is vital for successful fertilization, embryo development, pregnancy, and transmission of genetic material to the offspring. DNA fragmentation is the most frequent DNA anomaly present in the male gamete that has been associated to poor semen quality, low fertilization rates, impaired embryo quality, and preimplantation development and reduced clinical outcomes in assisted reproduction procedures. This work summarizes the causes of fragmentation in the spermatic DNA, and its relation with seminal parameters, male aging, and results in assisted reproduction procedures.
Part of the book: New Discoveries in Embryology
Improving Embryo Quality by Strictly Controlling IVF Laboratory Environment
In recent years, several changes have been made in different aspects of in vitro fertilization to improve embryo quality and ultimately the clinical outcomes in assisted reproduction technology (ART). These approaches include improvements in air quality inside the lab to ensure VOCs-free air, use of tri-gas incubator and embryo-tested devices and plastics, adequate control of pH and osmolarity of culture media, and strict quality control that allows an adequate development of the embryos until blastocyst stage. Other strategies to improve the embryo quality during in vitro culture include volume reduction of drop culture media, and individual or group culture of embryos. This work summarizes several strategies to improve embryonic quality during their in vitro culture in assisted reproduction procedures.
Part of the book: Embryology Update