Galba Maria Campos-Takaki

By Raquel Diniz Rufino, Juliana Moura de Luna, Leonie Asfora Sarubbo, Lígia Raquel Marona Rodrigues, José Antônio C. Teixeira and Galba Maria de Campos-Takaki

Part of the book: Practical Applications in Biomedical Engineering

By Thayza Christina Montenegro Stamford, Thatiana Montenegro Stamford-Arnaud, Horacinna Maria de Medeiros Cavalcante, Rui Oliveira Macedo and Galba Maria de Campos-Takaki

Part of the book: Practical Applications in Biomedical Engineering

By Patrícia Mendes de Souza, Thayse Alves de Lima e Silva, Marcos Antonio Barbosa Lima, Luciana de Oliveira Franco, Manfred Schwartz, Paulo Henrique da Silva, Lúcia Roberta Barbosa, Aline Elesbão do Nascimento, Kaoru Okada and Galba Maria de Campos- Takaki

Biodesulfurization (BDS) is one of the most promising technologies used together with traditional hydrodesulfurization (HDS) to reduce the sulfur content of fossil fuels. In this research study, a strain of Cunninghamella elegans (UCP 596) was isolated from mangrove sediments to metabolize an organosulfur dibenzothiophene (DBT) compound in the concentrations of 0.5 and 1 mM and transform to DBT sulfone (DBT-5-dioxide), followed by dibenzothiophene 5,5-dioxide and 2-hydroxybiphenyl metabolites, thus suggesting the use of the “4S” metabolic pathway. The fungus also degraded the DBT completely in the first 24 h of growth on a 2.0 mM DBT concentration by angular deoxygenation, which suggests that a new second metabolic pathway was used. The DBT was consumed as the carbon source, and the sulfur was removed in the form of sulfite ion. A new product, benzoic acid, was formed at the end of the catabolism of DBT by C. elegans using an angular route.

Part of the book: Recent Insights in Petroleum Science and Engineering

By Thayse A.L. Silva, Manfred Schwartz, Patrícia M. Souza, Ian Garrard, Galba M. Campos-Takaki and Elias B. Tambourgi

Dibenzothiophene (DBT) is a typical recalcitrant thiophenic sulfur component of fuels, and its desulphurization has been a model reaction in the treatment of these compounds. Based on this information, the potential of Pseudomonas fluorescens (UCP 1514) on the desulfurization of dibenzothiphene was studied, in order to use it for reducing the sulfur content of diesel oil in compliance with environmental regulations. The result of biodegradation by the bacteria was determined by undertaking high-performance liquid chromatography of the metabolites produced. These can also be identified by gas chromatography with a mass spectrometry detector, and doing so revealed a sulfur-free product, biphenyl, as the final product of the degradation process. The results showed a decrease of 73% in dibenzothiophene content, which means that P. fluorescens removes sulfur from dibenzothiophene with a good selectivity to form biphenyl. These promising results indicate that P. fluorescens has an interesting potential to degrade sulfur-containing compounds in diesel oil and thereby could help in removing sulfur content from diesel oil. The process of microbial desulfurization described herein can be used particularly after carrying out hydrodesulfurization. Consequently, the sulfur content could be reduced even further. Applying P. fluorescens UCP 1514 in dibenzothiophene could help to understand the nature of the biodegradation process and to achieve the regulatory standards for sulfur level in fossil fuels.

Part of the book: Recent Insights in Petroleum Science and Engineering