Abstract
Astrocytes are the most abundant cells of the central nervous system. These cells are of diverse types based on their function and structure. Astrocyte activation is linked mainly with microbial infections, but long-term activation can lead to neurological impairment. Astrocytes play a significant role in neuro-inflammation by activating pro-inflammatory pathways. Activation of interleukins and cytokines causes neuroinflammation resulting in many neurodegenerative disorders such as stroke, growth of tumours, and Alzheimer’s. Inflammation of the brain hinders neural circulation and compromises blood flow by affecting the blood–brain barrier. So the oxygen concentration is lowered, causing brain hypoxia. Hypoxia leads to the activation of nuclear factor kappa B (NFkB) and hypoxia-inducible factors (HIF), which aggravates the inflammatory state of the brain. Hypoxia evoked changes in the blood–brain barrier, further complicating astrocyte-induced pathogenesis.
Keywords
- astrocyte
- hypoxia
- inflammation
- neuroinflammation
- brain
1. Introduction of astrocytes
In the nervous system, astrocytes are isotypes of neuroglia, also identified as astrocytic cells. They are Star-shaped; their countless progressions enclose synopses prepared by nerve cells. A particular astrocytic cell can simultaneously act together with the human being by two billion synapses. These specialized glial cells are more numerous than neurons by above fivefold. They closely tile the central nervous system (CNS) and apply for multiple important diverse roles in the energetic CNS. Astrocytes react to all methods of CNS offences through a procedure stated as reactive astrogliosis, which has developed a pathological mark of CNS fundamental abrasions. Two main subtypes of astrocytes are classified based on their structural and anatomical position. Those names are protoplasmic astrocytes and fibrous astrocytes. Protoplasmic astrocytes seem to spread equally in the interior of cortical grey matter, while the fibrous astrocytes are structured with white matter regions [1]. Astrocytes of the brain and spinal cord are very different in morphology and function. Brain cells (astrocytes) are accountable for the homeostasis of ions and neurotransmitters in the synaptic cleft, native metabolic sustenance, and relief of sensitive oxygen species. Pathology of many nervous disorders containing neuropsychiatric and neurodegenerative syndromes is well-defined by loss of homeostatic role. Astrocytes play a significant role in the homeostasis of the central nervous system (CNS). Brain cells or astrocytes are extremely diverse cells that regulate the network, emergence and function, and homeostasis. Since it’s involved in protective astrogliosis, it’s become an essential component of neuropathology. Most neuropathology astroglial cells are impacted by degenerative alterations that inhibit their functional and neuroprotective capacities, allowing the pathology to proceed [2]. Astrocytes play an important role in data handling, and communicative mechanism proficiencies of brain circuits are unknown. Around all research studying the correlation among astrocyte cells’ structure and function concentrates on its influences on nervous system activity and flexibility under functional and syndrome conditions. At synapses, a collective subject important to these outcomes is that astrocytes analyse, respond to, and control glutamate release and post-synaptic activity.
Removal and postponement of PAPs in reaction to glutamate improve post-synaptic responses, inhibit trans-synaptic activation, and prevent additional glutamate proclamation. Still, astrocyte operational flexibility exchange is not recognized upon declaration of other GABA, dopamine, somatostatin, serotonin, acetylcholine, etc. (neurotransmitters) [3]. The appearance of glial fibrillary acid protein (GFAP) has become a typical indicator for immunohistochemically astrocytic cells [4]. Research on transgenic mice showed that the appearance of GFAP isn’t necessary for the usual form and role of the furthermost astrocytic cell in the energetic, nervous system of transgenic mice. Still, it’s essential to develop reactive astrogliosis and glial scar development. Over and above, concerning the procedure of GFAP as an astrocyte indication, it’s compulsory to pay attention that GFAP expression isn’t limited to protoplasmic and fibrous astrocytes. In the interior of the nervous system, GFAP is too expressed by numerous cells that can be reflected as part of prolonged astroglial cells. On the outer side of the nervous system, GFAP is articulated extensively in countless nerves via a range of cell forms Figures 1–4 [5].
2. Anatomical association
Astrocytes tile the whole CNS in a touching, non-covering, organized, and efficient way. Comparative individual astrocyte areas show up liable to exist in white matter. However, this has not yet been as broadly written about. Astrocytes are giant, intricate, and different from astrocytes in rodents. Astrocytes show controlled proliferation in intracellular calcium absorption [Ca++], which signifies a method of astrocyte excitability. An enormous suggestion is now obtainable that these delimited proliferations in astrocyte [Ca++] remain of purposeful importance in astrocyte and astrocyte-neuron intercellular communication. The advice is that calcium signalling permits astrocytes to show a direct role in synaptic transmission. It’s worth noting that astrocytes do communicate with one another via gap junctions generated by connexins. Gap junctional partnering of astrocytes into multicellular systems may contribute to normal function and CNS disorders. The suggestion is that calcium signalling permits astrocytes to show a natural interest in synaptic transmission.
3. Astrocyte-induced pathogenesis
ASTROCYTIC cells are central homeostatic and protective cells of the nerves, and every kind of astrocyte plays an integral part in neuropathological changes. Hence, the decline in nerve cells or astrocytes causes a disease-permissive landscape and triggers nerve cell malfunction, nerve cell death, and nerve cell deficiency. Glia cells are essential for sustaining nerve function, and nerves survive bodily procedures and pathology [6]. Most essential findings concerning astrocyte’s functional significance depend on the dead animal model research. It’s given a durable but incomplete base for a complete astrocyte role in physiopathology [7]. They are categorized into three kinds that are reactive astrogliosis, Astro-degeneration with astroglial atrophy, and pathological remodelling and loss of function of astrocytes. Altogether these pathological feedbacks proceed together. It’s categorized on the base of neuroanatomical and severity. According to neuroanatomical, astrocytes are further distributed into isomorphic and isomorphic astrogliosis [8]. The isomorphic astrogliosis conserves astroglial defensive areas that are changeable.
In contrast, anisomorphic astrogliosis continues through the destruction of the defensive regions, cell relocation and territorial overlap, development of astroglial palisades, and eventually scar formation. While in severity, astrogliosis is categorized into slight to adequate astrogliosis, severe diffuse astrogliosis and severe astrogliosis by dense scar development [9]. Astroglial atrophy is mainly noticeable in major psychiatric illnesses. For example, schizophrenia, a primary depressing condition, Wernicke–Korsakoff encephalopathy, and addictive disorders decrease the storing concentration of astrocytic cells. The conclusion is furthermost particularly accompanied by glutamate-glutamine shuttle and glutamate homeostasis; both are impaired in these conditions [10]. It promotes several leukodystrophies, especially Alexander disease, megalocephalic leukoencephalopathy with subcortical lumps or disappearing white matter disease, in which the astrocyte-pathy pledges destruction of the white matter [11]. It also describes mesial temporal lobe epilepsy, in which astrocytes obtain abnormal cell structure, decreases gap junction coupling, and decrease Kir4.1 channel expression; these alterations weaken K+ homeostasis, contributing to seizure start [12].
4. Pathology of astroglia in neurological diseases
Reactive astrogliosis and glial scar realization are noticeable structures of CNS trauma and are progressively involved by playing significant parts in the decisive prolonged medical result. Glial formation of scar and severe reactive astrogliosis at the location of the neurons trauma are glowing familiar to inhibit axonal regeneration and are extensively observed as harmful to medical results. Contagions of the CNS triggered by microbes, parasites, fungi, and viruses are categorized into inflammation of meninges, active tissues of the brain, or (pus-filled pocket of infected material in your brain) cerebral abscess. Not all germs can attack the nervous system. Somewhat, merely confirmed neurotropic parasites, fungi, bacteria, and viruses can enter obsessed by the cerebral and vertebral column. Utmost of the microbe are efficiently stopped by the cerebral obstacles [13].
The SAE (Sepsis-associated encephalopathy) explicitly uses a scientific disorder linked through the common cerebral disorders that progresses in sepsis to the lack of core contagion of the neural tissue. In the cerebral parenchyma, sepsis is frequently linked with the production of inflammations and tiny inflammation directly related to the SAE. Particularly in the initial phases, the disease associated with structural infection remains frequently linked through ‘sickness behaviour’ [14].
Dense metals are a source of extreme cephalic disorder with intellectual deficiencies, mainly targeting neuroglia. Heavy metals that are manganese, lead, aluminium or mercury primarily gather into an astrocytic cell by diverse plasma lemma transporters. Overall, it’s also a down-regulating astroglial expression of glutamate transporters, reducing glutamate permission and activating excitotoxicity [15]. Aluminium toxic encephalopathy is demonstrated via mental losses, communication variations, seizures, and flapping wrist shake (asterixis) [16]. It disturbs the cerebrum due to liver encephalopathy, which is described by mental and developmental damage signs including misperception, amnesia, bad temper, and alterations in perception, such as fatigue and sleepiness. Cerebral swelling, unconsciousness, and death also occur in the severity of Hyperammonemia. In stroke, a blood vessel ruptures that restricts blood supply to the brain or parts of the brain due to a systemic decrease of vascular occlusion in blood supply, all-cause disruptions in blood flow. This is known as brain ischemia. As a result, cerebral ischemia could be either focal or global, the latter of which can lead to a stroke. In stroke, astrocytes serve as both neurotoxic and neuroprotective agents, complicating and diverse astrgliopathology [17]. In cases of Congenital Glutamine Deficiency with Glutamine, Synthetase Mutations newborns expire in a while after delivery. The prominent pathophysiological contrivance is related to the weakened capacity of astrocytes to yield glutamine, which disturbs excitatory and inhibitory conduction; furthermore, lacking glutamine synthetase cannot accurately decontaminate ammonium. Pyruvate carboxylase is principally articulated in neuroglia. Pyruvate carboxylase deficit is an autosomal recessive disease linked to a diminished metabolic rate. The warning sign comprises delay of cerebral growth, persistent seizures, and increased plasma acidity.
Aceruloplasminemia is a congenital condition of iron metabolism due to the deficiency of ceruloplasmin action. Most important abrasions describe this disorder as neuroglia, which disturbs their structure and consequences in the presence of frothy spheroid forms at the vascular end feet. Aceruloplasminemia is also linked with brain demise and the exterior of iron deposition [18].
Alexander disease is an exceptional, long-lasting, and ordinarily neurodegenerative severe condition. Its consequences on or after a dominant gain-of-function mutation of the gene encoding GFAP. It is sub-categorized into Type I and types II [19]. Autism Spectrum Disorders (ASD) are a few medically introverted conditions connected to cerebral inadequacies. Astrocytes are accountable for neuroprotection and detoxifying harmful bodies like receptive oxygen species. The principle procedure of ASDs is undoubtedly associated with brain network mutation and abnormal neurotransmission during the undeveloped turn of events [20]. In Down syndrome, the thickness of astrocytes is fundamentally diminished in the cortex with diminished capacity to uphold synaptogenesis and neuronal development appropriately. Astroglial asthenia, loss of homeostatic capacities, atrophy, and perhaps pathogenic remodelling are all related to schizophrenia, although reactive alterations are not. Epilepsy, mood disorders, and addictive disorders are linked with astrogliopathology [12].
5. Astrocyte up-regulation
Astrocyte is the essential part of the blood–brain barrier (BBB) that can be damaged by traumatic brain injury (TRI) or ischemia. Astrocytes provide the morphological and physiological link between neural networks and cerebral circulation. Astrocytes have the power to adjust blood flow to the brain to keep the brain parenchyma’s PCO2 and PO2 steady [21]. The formation of ATP in the brainstem through local astrocytes aids respiration and counters hypoxia-induced respiratory network depression. Hypoxia-induced alterations in brain inflammation, neuroprotection, and blood–brain barrier permeability against ischemic injury appear to be mediated by astrocytes. Astrocytes play a critical function in neuronal function in everyday situations and pathological conditions when the supply of oxygen to the brain is disrupted [22].
The telomerase reverse transcriptase (TERT) gene is linked to cell injury and stress resistance. After hypoxia-ischemia, protein and TERT mRNA were increased in neurons after a few days but moved to astrocytes [23]. TERT overexpression decreased astrocyte multiplication by upregulating the cell-cycle regulatory protein p15. While neurons were cultured with precondition medium from astrocytes with TERT inhibition contrasted to neurotrophin-3 expression, TERT overexpression in astrocytes decreased, resulting in higher death [24]. In TERT-overexpressing brains with hypoxia-ischemia, neuronal damage and Ki67-positive astrocytes were also suppressed [25].
Matrix metalloproteinase (MMP)-9 is an endo-peptidase that plays a crucial role in Blood–Brain Barrier proteolysis post-trauma and leads to cell death with persistent convulsions [26]. Activation of mitogen-activated protein kinases (MAPKs) in astrocytes can be caused by thrombin, oxidative stress, tissue plasminogen activator, or tumour necrosis factor- α and includes stimulation of MMP-9. In astrocytes, albumin causes a rise in MMP-9 synthesis, which requires ROS formation and motivation of the MAPK pathway [27]. These results add albumin to signalling molecules that activate MMP-9 in astrocytes alongside thrombin. These findings connect albumin to MMP-9-mediated cellular mechanisms such as intracerebral haemorrhage, neuronal damage, dendritic remodelling, and epileptogenesis [28].
Stroke and Traumatic Brain Injury (TBI) are frequently linked with hypoxia, which causes glial initiation. Glial cells, particularly astrocytes, play an essential part in stress prevention and the homeostasis of the CNS by offering structural and metabolic stability [29]. Hypoxia can cause astrocyte homeostasis to be disrupted, resulting in cell enlargement. Wnt pathway suppression was the most substantially disrupted signalling pathway in the mechanism of hypothermia-induced responses in human astrocytes after oxidative stress activation and hypoxia [30]. Global suppression of Wnt signalling can be troublesome because of its essential role in controlling critical mechanisms associated with the functional regulation of immune and stem cells and its effect on post-mitotic neuronal and glial cells [31].
6. Brain hypoxia
Molecular oxygen (O2) is required for most organisms on the planet because it supports intracellular biogenesis and is utilized by several metabolic activities. As a result, low oxygen level (hypoxia) is a crucial stress factor that usually disrupts aerobic species’ lives and is a common feature of pathologic conditions such as cardiovascular abnormalities, inflammation, wounds, bacterial infection, and cancer [32]. Since it is required throughout breathing, oxygen is essential in life. In oxidative phosphorylation, O2 acts as the ultimate electron acceptor, raising the possibility of reactive oxygen species formation (ROS). ROS interact with biological macromolecules, changing their metabolic or physical characteristics and causing cell death or malfunction [33].
To sustain a wide range of cellular functions to secure life, organism cells require sufficient oxygen. The oxygen concentration in the body and localized tissues fall (hypoxia) whenever the need for oxygen increases, resulting in a physiological crisis that compromises physiological processes and survivability. Organisms have formed an effective and quick oxygen sensing system called (hypoxia-inducible factors (
Arterial and central chemoreceptors detect changes in the oxygen content in the external environment. The brainstem’s medulla, beneath the respiratory centres, contains central chemoreceptors. The carotid and aortic bodies include arterial chemoreceptors [35]. The stimulation of arterial chemoreceptors promotes neurotransmission and alters the function of neprilysin (NEP), a neutral endopeptidase that changes the biological response to hypoxia by hydrolytic component P [36]. Neuroendocrine cells in neuroepithelial bodies also govern oxygen sensing by imposing chemosensitivity, critical for oxygen sensing in the early stages of life. These exciting chemoreceptors enhance sympathetic nervous activity (SNA) and the systemic and arterial pulmonary blood flow to receive enough oxygen. Therefore, in organism cells, the expression of many adapted genes is activated to improve the oxygen supply and enable anaerobic ATP production. Hypoxia-inducible factors regulate these hypoxic responses (
There is a difference between sustained and intermittent hypoxia. Mitochondrial respiration utilizes more than 90% oxygen in humans during every day physiological situations [38]. The remaining (10%) oxygen is used to degrade
7. Role of HIF 1a in causing hypoxia in the brain
A transcription factor that binds to specific nuclear cofactors and transactivates several genes, causing a range of adaptive responses in response to low oxygen levels in the body, is called Hypoxia Inducible Factor
The brain is the most vulnerable organ to hypoxia, resulting in coma, convulsions, cognitive impairment, other neurological impairments, and brain death if left untreated [44]. Cardiac arrest, asphyxia, or systemic metabolic abnormalities affecting the blood’s oxygen content, systemic hypoxia, severe anaemia, and systemic hypotension can lead to hypoxic brain damage [45]. Hypoxia-induced autophagy is linked to the HIF-1 signalling pathway. According to studies, hypoxic preconditioning protection is lost in HIF-1α knockout mice exposed to neonatal hypoxia/ischemia [46]. HIF1α hydroxylation is prevented by blockage of prolyl- and asparaginyl-hydroxylases in hypoxic environments. Prolyl-hydroxylase inhibitors reduce HIF1α breakdown, resulting in fast
PI3K (Phosphatidylinositol 3-kinase) and Akt (protein kinase B) signalling pathway is related to hypoxia-ischemia injury as it increases the phosphorylation of downstream molecules such as apoptosis-related family members, transcription factors, mammalian target of rapamycin (mTOR), and glycogen synthase kinase-3. Phosphatase and tensin homologue (PTEN) is a lipid phosphatase that inhibits the PI3K/Akt pathway by hydrolysing PIP-3 to PIP-2 and preventing downstream p-Akt. PI3K and its downstream effector. Akt is a member of a well-studied family of signal transduction enzymes that regulate cellular activation, inflammation, and apoptosis [49].
8. Role of hypoxia in activating inflammatory pathways such as interleukins, cytokines, NFk-B1
Chemokines and cytokines are low-molecular-weight proteins produced mostly by lymphocytes and macrophages. As neurotransmitters and hormones, they mediate intracellular and extracellular interactions in an autocrine, endocrine, and paracrine manner. They regulate various biological activities, including local and systemic anti- and pro-inflammation, chemotaxis, metabolism, cellular proliferation, and tissue repair, by adhering to certain cell surface receptors [50].
cAMP-mediated signalling pathways might be changed in the presence of HIF1A, causing inflammatory-like processes to worsen. Only in the presence of Ni-induced hypoxia-inducible factor 1 (
9. Effect of hypoxia on astrocyte functioning
Astrocytes have a crucial role in maintaining the normal oxygen levels in the brain. If the PO2 goes less than 17MMHg, astrocytes robust the Calcium ions into the brain; they act as the source of ATP in the hypoxic state of the brain. Astrocytes also have the potential to sense an increase in PCO2 levels too [21]. This chemosensation helps the cells to provide the astroglial networks with ATPS that help spread Ca + 2 activation and excitation. It also increases breathing to maintain homeostasis. It suggests that ATP released helps to keep living in the face of the hypoxia-evoked depression of the respiratory network [55].
In the previous century, it has also become clear that astrocytes can protect neurons under hypoxia conditions. The potential process is similar to “hypoxic preconditioning,” in which a temporary interval of moderate hypoxia protects neurons from subsequent ischemia episodes that are generally fatal [56]. Mild hypoxia synthesizes several protective astrocytic factors that help neurons survive. Hypoxia increases the production of specific proteins, such as connexin 43, which may promote ATP/adenosine transit towards the interstitial space [57]. Astrocytes release erythropoietin in reaction to hypoxia, which has a significant neuroprotective impact. The erythropoietin expression in astrocytes is increased once hypoxia-inducible factors are activated. HIF-1α and HIF-2α are two transcription factors. [58]. In an adult brain, astrocytes can alter and monitor synaptic functionality. It was believed that synaptic plasticity is solely based on neurons, but in recent research, it has been found that the glial network and astrocytes alter synaptic transmissions [59]. The activation of metabotropic receptors modulates synaptic alterations by astrocytes. It helps release glutamate, gliotransmitter ATP, and D-serine, which act on neurons [60]. As per Astrocyte-induced pathogenesis, astrocytes can cause adenosine accumulation that affects glial cells and cause sleep deprivation and cognitive impairment [61]. Experimental investigations have found that the astrocytes sense synaptic activity with the help of astrocytic calcium. Astrocytes elevate their Ca + levels to sense neural activity with the help of the Gq-coupled protein pathway [62].
10. Conclusion
Astrocytes play a significant role in the homeostasis of the central nervous system. Astrocytic cells are central homeostatic and protective cells of the nerves. The decline in nerve cells or astrocytes causes a disease-permissive landscape and triggers nerve cell malfunction, nerve cell death, and nerve cell deficiency. Astroglial atrophy is mainly noticeable in major psychiatric illnesses. Severe reactive astrogliosis and glial scar formation at the location of the neurons trauma are glowing familiar to inhibit axonal regeneration. More research into the processes of Astrocytes protection, particularly the substances they produce, will give crucial insights into how to protect the Blood–Brain Barrier throughout trauma and neurological condition.
Moreover, its expression timing in astrocytes is essential to determine the influence of hypoxia-induced signalling on stroke volume. Furthermore, in addition to the impacts of hypoxia-signalling in astrocytes on neuron viability, it seems necessary to consider how such alterations will affect astrocyte viability. We must fully comprehend how to lessen the harm caused by stroke if we can better define the various consequences of hypoxia signalling in astrocytes.
Funding
The research received no specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Competing interests
The authors have declared no competing interests.
Ethical approval
The study does not require any ethical approval.
Availability of data and materials
No data was generated in the current study.
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