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Can Genus Trichoderma Manage Plant Diseases under Organic Agriculture?

Written By

Kishor Chand Kumhar, Dalvinder Pal Singh and Anil Kumar

Submitted: January 22nd, 2022 Reviewed: February 16th, 2022 Published: April 5th, 2022

DOI: 10.5772/intechopen.103762

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Trichoderma Edited by Fernando Cezar Juliatti

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Trichoderma [Working Title]

Dr. Fernando Cezar Cezar Juliatti

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Abstract

Organic agriculture has been coming up as one of the promising segments of crop production systems in India. There are numerous reasons for it, however; human health, sustainable environment, soil health, etc. are the important ones. As per the latest information, India has about 1.5% of total cultivable land under organic agriculture. The occurrence of plant diseases in this crop production system is one of the limiting factors. For the management of plant diseases in organically grown crops, there are limited resources since there is a restriction on the use of synthetic fungicides. Under such a situation, bio-pesticides have the potency to take care of plant diseases. Although there are certain fungal and bacterial candidates well efficient in controlling diseases, genus Trichoderma has occupied a prestigious position among them. It is capable of managing seed and soil-borne plant diseases. Presently it is available in wettable powder (WP) and liquid formulations in variable concentrations for the application.

Keywords

  • Trichoderma spp.
  • plant diseases
  • organic agriculture
  • mode of action
  • formulation

1. Introduction

The interest in organic crop production has been increasing day-by-day since the last decade because of the increased negative impact of conventional agriculture production systems. Production of crops adopting the inorganic agricultural inputs primarily agrochemicals, which has been used starting from seed treatment till harvesting of the crop for providing protective umbrella against various diseases. The frequent and injudicious application of inorganic agrochemical inputs has invited several environmental and health-related problems.

The demand for organic food commodities has been increased tremendously and hence researchers, as well as growers, have been focusing their attention towards organic crop production wherein, the use of inorganic inputs is completely avoided. However, successful crop production through this system faces various biotic and abiotic challenges too.

Organic farming was first initiated by British botanist Sir Albert Howard in 1905. However, in India, it was initiated during the late 90s. The government of India, in the year 2001, implemented the national program for organic production (NPOP) for the promotion of organic farming. Organic farming was started in several states. Sikkim has converted cent percent of the agriculture into an organic state in this country. Presently, India has about 2.3 million hectares under it [1]. with a production of about 1.70 million MT certified organic products with voluminous export of different organic food items [2].

The occurrence of plant diseases is one of the important concerns in organic agriculture. Various fungi are notable/major phytopathogenic fungi causing huge crop losses [3]. For the management of plant diseases, plant protection measures start as early as seed treatment and would continue until crop harvesting in different crops.

In the recent era, organic agriculture is getting huge popularity and adaptability owing to its several beneficial aspects. The organically produced agricultural commodities ensure pesticide-free items which are fit for human consumption. Under such a crop production system, taking care of plant diseases is one of the major challenges. There are limited plant protection options due to the restricted use of synthetic fungicides in it. It emphasises the use of on-farm inputs for almost all requirements. Under such circumstances, the application of Trichodermaspp. could be an ideal alternative to handle plant diseases. Since the last couple of decades, genus Trichodermahas occupied a reputed position among the microbes possessing disease management potency. As a biocontrol agent, the antagonistic property of Trichoderma viridewas recognised [4] while working with the damping-off of citrus seedlings caused by Rhizoctonia. Various chemical fungicides were the most dominant during the green revolution era in the 60s and 70s and hence Trichodermacould not significantly attain popularity among the growers at the grass-root level. Due to excessive usage of chemical fungicides numerous problems related to the environment, soil and human health ecosystem, resistance development, etc. have emerged as a big challenge before everyone, including scientists, consumers, growers, and the overall associated environment. Therefore, majority has been preferring pesticide-free food commodities for the last so many years. It is good news or sign that usage of chemical fungicides has been coming down and usage of biopesticides has been going up nationally as well as internationally [5]. Currently, in India, there are several hundred manufacturers of Trichodermapossessing the registration certificate from the Central Insecticide Board and Registration Committee [6]. They are manufacturing and marketing the Trichoderma-based products with variable active ingredients, the dose of application in diversified crop avenues. Although there are four-five species of this antagonist suitable for the management of plant diseases, however only two species namely, T. virideand T. harzianumhave been made available to the end-users. The plus point with this antagonist is that it can be used in a wide range of crops, such as cereals, pulses, vegetables, horticultural, and plantation crops [7, 8, 9, 10, 11]. Its wettable powder (WP) and liquid formulations are popular in the market; however, the first one is dominant. In addition to managing the phytopathogen, it is capable of promoting the vegetative growth of plants. Being a natural fungus, it is safer for the environment as well as consumers, capable of adjusting and performing under variable conditions. This chapter highlights various important information related to this genus.

This chapter mainly focuses on the application of Trichodermaspp. for the management of plant diseases. Organic crop production excludes the use of synthetic/inorganic fungicides for the control of plant diseases.

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2. History and journey of Trichodermaspecies

Before touching its actual uses in agriculture, it becomes imperative to focus on some important aspects related to it. For the first time, Persoon introduced the name Trichoderma[12]. In 1865, the Tulasne brothers reported teleomorph (sexual stage) of T. viridePers as Hypocrea rufa[13]. Till 1969, there were only one species, i.e., T. virideof genus Trichoderma[14].

In 1969, Rifai made out nine ‘aggregate species’ namely, (1) T. harzianumRifai, (2) T. viride,(3) T. hamatum(Bonord.) Bainier, (4) T. koningii(Oudem.) Duché & R. Heim, (5) T. polysporum(Link) Rifai, (6) T. piluliferumJ. Webster & Rifai, (7) T. aureovirideRifai, (8) T. longibrachiatumRifai, and (9) T. pseudokoningiiRifai. In the early 1990s, [15, 16, 17] identified five sections and 27 biological species within the genus Trichoderma.The introduction of molecular techniques contributed to the greater extent in identifying the species comparatively more precisely and hence from the late 1990s up to the year 2002, the number of Trichodermaspecies increased to 47 [18].

Till 2005, the International sub-commission on Trichoderma/Hypocrealisted 104 species on the basis of phylogenetic analyses [19]. Till the year 2015, there are 252 species one variety and one form. In addition, T. neocrassumSamuel (syn. Hypocrea crassaP. Chaverri & Samuels) and T. patellotropicumSamuels (syn. Hypocrea patellaf. tropicaYoshim.Doi) were proposed as two new names [20].

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3. Interaction of Trichodermawith phytopathogens of agricultural crops

Hyperparasitism, competition, and antibiosis are the important mechanisms of this genus through which it interacts with phytopathogenic fungi. This antagonist is well efficient to colonise in variable ecological niches [21, 22, 23].

3.1 Hyperparasitism

In Hyperparasitism, the Trichodermadirectly contact with a pathogen and finally cause the death of pathogen cells death [23]. For this purpose, the Trichodermasynthesise cell wall degrading enzymes (CWDE) namely, cellulase, xylanase, pectinase, glucanase, lipase, amylase, arabinase, protease [24], and lytic enzymes. These enzymes degrade the pathogen cell walls, composed of chitin and glucan polysaccharides. Among the chitinolytic enzymes β-N-acetyl glucosaminidase, endochitinase, and chitobiosidase are of key importance responsible for the degradation of the cell wall of other plants pathogenic fungi, which are produced by T. harzianum, T. atrovirideP. Karst, and T. asperellumSamuels, Lieckf. & Nirenberg [25]. Several volatile metabolites, such as 6-n-pentyl-2H- -pyran-2-one (6-PAP), are produced by Trichodermafor plant protection [26]. The β-1,3- and β-1,6-glucanases enzymes determine the hyperparasitic capability of Trichodermato react on species of Phytophthorasp. and Pythium[27, 28]. Proteolytic enzymes, namely, endo- and exoproteases of Trichodermaresponsible for enzyme secretion for the control of Botrytis cinerea, Rhizoctonia solani,and Fusarium culmorum[29, 30]. Ceratin cellulase enzymes, such as exo-β-1,4-glucanases, endo-β-1,4-glucanases, and β-glucosidases, produced by antagonists also play a significant role in hyperparasitism [31].

3.2 Competition

Competition of Trichodermaspp. with plant pathogens is another mode of interaction that helps in the control of plant diseases. This phenomenon takes place for utilisation of nutrients, occupying ecological position or infection sites on plant roots. Certain Trichodermastrains produce siderophores, i.e., iron-chelating compounds. Through siderophores, antagonist traps iron from the associated environment and creates nutrient deficiency due to which the growth of pathogenic fungi, such as B. cinerea,is hampered. The Trichodermacreates an acidic environment that has a negative effect on the growth and development of pathogenic fungi and aggressively colonises the host plant’s roots due to the enhanced activity of the hydrophobins [9].

3.3 Antibiosis

It is a biological interaction between two or more organisms that is unfavourable to at least one of them; it can also be an antagonistic association between an organism and the metabolic substances produced by another. Antibiosis in relation to Trichodermafungi is a specific mechanism of antagonistic interactions with other plant pathogenic fungi. This phenomenon is based on the generation of secondary metabolites, which exhibit an inhibitory or lethal effect on a parasitic fungus. From the genus Trichoderma, over 180 secondary metabolites have been characterised so far, representing different classes of chemical compounds [32, 33]. Such compounds can be divided into volatile antibiotics, water-soluble compounds, and peptaibols. Volatile antibiotic, (6PAP (6-pentyl-α-pyrone) produced by T. viride, T. harzianum, and T. koningii, plays a major role in the biocontrol of Botrytis cinerea, R. solani, and Fusarium oxysporum.

Another category of antibiotics is Peptaibols. They are polypeptide antibiotics comprising of 500–2200 Da, rich in non-proteinogenic amino acids, specifically alfa-aminoisobutyric acid, and their characteristic attributes include the presence of N-acetylated ends and C-end amino alcohols. Peptaibols exhibit potent activity against a number of fungi (Table 1).

Trichodermaspp.Produced Peptibols antibioticsReference
T. viridetrichotoxins A and B, trichodecenins, trichorovins, and trichocellins[34]
T. harzianumtrichorzianins A and B, trichorzins HA and MA[34]
T. longibrachiatumtricholongins BI and BII, and longibrachins[34]
T. koningiitrichokonins[34]
T. atrovirideatroviridins A-C and neoatroviridins A-D[34]
T. koningii, T. harzianum, T. aureoviride, T. viride, T. virenskoningins, viridin, dermadin, trichoviridin, lignoren, and koningic acid[33]
T. lignorum, T. virens (G. virens), T. viride, and T. hamatummycotoxin[33]
T. harzianumharzianum A[35]

Table 1.

Peptibols antibiotics of Trichodermaspp. to control phytopathogens.

In Trichodermafungi, the activity of antibiotics is enhanced with the activity of lytic enzymes. Their joint activity provides a higher level of antagonism compared to the activity of either enzymes or antibiotics alone [36]. Researchers found preliminary degradation of the cell walls in B. cinereaand F. oxysporumby lytic enzymes; it facilitated easier penetration of antibiotics to pathogen cells [8].

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4. Role of Trichodermaspp. as a biological control agent

Trichodermaspp. are the ideal option for safer management of phytopathogens. T. harzianumand T. viridehave been occupied the prime position and its highly exploited biological control agent for controlling soil-borne fungal diseases.

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5. Current status of Trichodermaformulations

There is a list of about 970 registered manufacturers with the Central Insecticide Board & Registration Committee (CIB & RC) for the manufacturing and marketing of biopesticides in India, out of which 558 are involved in Trichodermaspp. (Figure 1). The different formulations of this antagonist comprised of wettable powder (WP), wettable suspension (WS), aqueous suspension (AS), and liquid. The Trichodermaspp. formulations have been manufactured and marketed by private companies, government organisations, and NGOs. Presently, wettable powder (WP) and liquid formulations are available for use by end-users; however, WP formulation in different concentrations (0.5 to 6.0%) is dominant (Figure 2). The liquid formulations contribute nearly 2% of the formulations.

Figure 1.

Number of manufacturers of differentTrichodermaformulations.

Figure 2.

Status ofTrichodermaWP and liquid formulation manufacturers.

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6. Management through seed treatment with Trichodermaspp.

Seed treatment is an important and economical practice to manage the seed-borne phytopathogens at the initial stage with the minimum cost. For this purpose, a small quantity of desired input is required and this practice is very easy in handling. For seed treatment, the formulation of T. harzianumand T. viridecan be used. Seedlings of vegetable crops can be treated with T. virideand T. harzianumjust before transplanting into the main field (Table 2).

InputCropAdopted by
TrichodermaChilli seed treatmentUttarakhand*
T. virideChickpea seed treatmentMadhya Pradesh#
T. harzianumTomato seedling treatmentMeghalaya#
T. virideMaize seed treatmentMeghalaya#
T. virideBroccoli seed treatmentSikkim#
T. virideNursery soil treatmentSikkim#
T. virideTomato seed treatmentSikkim#
T. virideCapsicum seed treatmentSikkim#
T. virideChilli seed treatmentTamilnadu#
T. harzianumWheat seed treatmentUttar Pradesh#
TrichodermaWheat seed treatmentUttarakhand#
PseudomonasWheat seed treatmentUttarakhand#
Pseudomonas + TrichodermaPea seed treatmentUttarakhand#
Pseudomonas + TrichodermaChickpea seed treatmentUttarakhand#
Trichoderma
Pseudomonas fluorescence
Wheat seed treatmentDharwad#
TrichodermaPotato seed treatmentDharwad#

Table 2.

Seed treatment with promising Trichodermaspp. for organic agriculture.

National Centre of Organic Farming.


Indian Institute of Farming Systems Research.


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7. Management through soil treatment with Trichodermaspp.

Soil residential phytopathogens, such as Pythium, Phytophthora, Fusarium, Rhizoctonia, Sclerotinia, Macrophomina,play an important role in the development of various soil-borne diseases, such as root rot, wilt, and seedling rot of various crops. Management of such fungal phytopathogens can be achieved through the application of T. harzianumand T. viridethrough soil drenching with water, enriched farmyard manure, and vermicompost. There are several species of Trichoderma, however T. harzianum, H. lixii, T. atroviride, H. atroviridis, T. asperellum,and T. virensare potential biocontrol agents against phytopathogens [37]. Application of T. virideenriched FYM (5 kg/plant) to two-year guava saplings at the basin near the root zone resulted in decreased wilt incidence and better plant growth in terms of stem girth [38]. T. harzianumwhen applied (50 g/vine) in black pepper field it had effectively managed the foot rot disease. For the management of pomegranate wilt bio-formulation of T. viride(0.1 and 0.2%) was found significantly superior over the control. Soil application of T. harzianumplus T. virensis efficient in managing stem bleeding disease of coconut [39]. Five monthly applications of T. harzianum(50 g/plant) in bananas reduced 50% of the vascular discoloration index of Fusariumwilt disease and increased the yield [40]. T. asperellumfound inhibitory against Pythiumaph anidermatum, Pythium debaryanum, Sclerotium rolfsii,and S. rolfsii, Fusarium oxysporumf.sp. lycopersiciand Alternaria solani. Application of Trichoderma(@20 kg/ha) along with 2.0 tonnes castor cake/ha reduced nematode population and increased yield in pomegranate.

Soil application of silver nanoparticles synthesised from T. asperellumresulted in complete control of Fusariumwilt in cv. Grand Nain [41]. T. harzianumtalc formulation could control root rot disease of citrus up to 80%, and under field conditions [42]. Some strains of Pseudomonasspp. and Trichodermaspp. found effective in controlling wilt of banana, caused by F. oxysporumf.sp. cubense(Foc) race 1 under field conditions [43]. Isolates of Trichodermaand Aspergillus,when applied in the field for the control of guava wilt disease caused by F.oxysporumf. sp. psidiiand F. solani, could reduce disease incidence and promoted plant growth [38]. Antagonistic fungi Aspergillus niger, T. harzianum,and Penicillium citrinumwere found effective for the management of the wilt disease of guava [44].

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8. Management through foliar application of Trichodermaspp.

Foliar application of Trichodermaspp. is advisable for the management of airborne fungal and bacterial phytopathogens, such as species of Alternaria, Curvularia, Fusarium, Colletotrichum, Pestalotiopsis, Pyricularia, Puccinia, powdery and downy mildew pathogens of cereal, vegetable and other crops (Table 3). The potent candidates include T. virideand T. harzianum. Post-prune foliar application of T. harzianumand T. virideis a common practice in tea (Camelliasp) crop production in Darjeeling and the North East region of India [45]. When a combination of T. harzianumand Pseudomonas fluorescenswas sprayed before harvesting mango fruits, it had suppressed post-harvest fruit rot in dasheri mango [47]. Banana hands (cv. Grand Nain) when dipped in T. asperellumsuspension and packed without ethylene absorbent extended its shelf life up to 75 days at 13.5°C. There was no incidence of anthracnose and crown rot [41]. It was noted that T. viride, T. harzianum,and T. asperellumwere potential antagonists for the management of F. solaniand Pestalotiopsis theaecausing dieback and grey leaf spot disease of tea [48, 49]. Foliar spray of T. asperellumand T. atroviridecould manage the dieback disease of tea (Camelliasp) and enhance the vegetative growth in terms of more number of pluckable shoots [50].

InputCrop diseaseAdopted by
Trichodermaspp.Anthracnose of chilliUttarakhand*
Trichodermaspp.Rust, powdery mildew, and blight of peaUttarakhand#
Trichodermaspp.Wilt and blight of chickpeaUttarakhand#
T. harzianumBlack rust, brown rust, yellow rust, and leaf blight of wheatJharkhand#
T. harzianumor T. virideLoose smut of wheatJharkhand#

Table 3.

Foliar spray of Trichodermaspp. in organic agriculture.

National Centre of Organic Farming.


Indian Institute of Farming Systems Research.


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9. Management through paste application of Trichodermaspp.

Application of Trichodermapaste (20% w/v) is generally done after severe pruning operations (rejuvenation prune and medium prune) in tea plantations to protect the plants from airborne pathogens. Such an application can be useful in horticultural crops in which pruning is done.

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10. Important considerations for better performance of Trichodermaspp.

There are certain issues/concerns which should be taken into consideration for achieving desired benefits from the use of Trichodermaspp. Important concerns are—(1) reliable source of procurement, (2) assurance of fresh formulations, (3) avoidance of tank mixing with agrochemicals, (4) tank mixing with compatible bio formulations, (5) repeated application at the proper interval, (6) use of neat and clean separate sprayers for such formulations, (7) seeking technical advice from experts, (8) application during early morning and late evening hours, (9) procurement in well-ventilated store under lock and the key, and (10) quality assurance through certified agencies, such as IMO, Ecocert, Lecon, or any other approved agency.

11. Conclusion

T. harzianumand T. virideare efficient in controlling several plant diseases safely. When considering the interactions of Trichodermafungi, it was found that these antagonistic fungi have an advantageous effect on plants. Stimulation of plant growth and yield takes place in this interaction and the advantageous effects are seen in the production of vitamins, the increased availability of biogenic elements (nitrogen, phosphorus), the mobilisation of nutrients from the soil and organic matter, and the enhanced intensity of mineral uptake and transport. Furthermore, Trichodermafungi are capable of producing zeaxanthin and gibberellin, i.e., compounds accelerating seed germination. Managing plant diseases through these approaches could be safer for the agroecosystem, overall environment, soil health, and human health and would be the right step in sustaining crop production to meet the demand of the growing population.

Acknowledgments

We, authors, are highly thankful to Dr. Azariah Babu, Deputy Director (Research), Tea Research Association, North Bengal Regional Research and Development Centre, Nagrakata, Jalpaiguri, West Bengal for critically going through the chapter.

Conflict of interest

The authors declare no conflict of interest.

References

  1. 1. Available from:https://www.fibl.org/fileadmin/documents/shop/1150-organic-world-2021.pdf[Accessed: February 6, 2022]
  2. 2. Available from:https://apeda.gov.in/apedawebsite/organic/Organic_Products.htm[Accessed: February 6, 2022]
  3. 3. Agrios GN. Plant Pathology. 5th ed. Gainesville, U.S.A.: Elsevier, Academic Press, University of Florida; 2005. p. 922
  4. 4. Weindling R, Emerson OH. The isolation of toxic substance from the culture filterate ofTrichoderma. Phytopathology. 1936;26:1068-1070
  5. 5. Zaki O, Weekers F, Thonart P, Tesch E, Kuenemann P, Jacques P. Limiting factors of mycopesticide development. Biological Control. 2020;144:104220
  6. 6. Bio-Pesticide Registrant | Directorate of Plant Protection, Quarantine & Storage | GOI (ppqs.gov.in) [Accessed: February 6, 2022]
  7. 7. Harman GE. Myths and dogmas of biocontrol changes in perceptions derived onTrichoderma harzianumT-22. Plant Disease. 2000;84(4):377-393
  8. 8. Howell CR. Mechanisms employed byTrichodermaspecies in the biological control of plant diseases; the history and evolution of current concepts. Plant Disease. 2003;87(1):4-10
  9. 9. Benítez T, Rincón AM, Limón MC, Codon AC. Biocontrol mechanisms ofTrichodermastrains. International Microbiology. 2004;7(4):249-260
  10. 10. Smolińska U, Kowalska B, Oskiera M. The effectivity of strains in the protection of cucumber and lettuce against. Journal of Fruit and Ornamental Plant Research. 2007;67(1):81-93
  11. 11. Kaczmarek J, Jędryczka M. Characterization of two coexisting pathogen populations ofLeptosphaeriaspp., the cause of stem canker of brassicas. Acta Agrobotanica. 2011;64(2):3-14
  12. 12. Persoon CH. Disposita methodical fungorum. Romers Neues Mag Bot. 1794;1:81-128. [Accessed: February 6, 2022]
  13. 13. Tulasne L, Tulasne R. De quelques Sphéries fungicoles, àpropos d’un mémoire de M. Antoine de Barysur les Nyctalis. Annales des Sciences Naturelles; Botanique. 1860;13:5-19
  14. 14. Bisby GR.Trichoderma viridePers. ex fries, and notes on Hypocrea. Transactions of the British Mycological Society. 1939;23(2):149-168
  15. 15. Bissett J. A revision of the genusTrichoderma. II. Infrageneric classification. Canadian Journal of Botany. 1991;69(11):2357-2372
  16. 16. Bissett J. A revision of the genusTrichoderma.III. Section Pachybasium. Canadian Journal of Botany. 1991;69(11):2373-2417
  17. 17. Bissett J. A revision of the genusTrichoderma. IV. Additional notes on section Longibrachiatum. Canadian Journal of Botany. 1991;69(11):2418-2420
  18. 18. Kullnig-Gradinger CM, Szakacs G, Kubicek CP. Phylogeny and evolution of the fungal genusTrichoderma: A multigene approach. Mycological Research. 2002;106(7):757-767
  19. 19. Druzhinina IS, Kopchinsky AG, Kubicek CP. The first 100Trichodermaspecies characterized by molecular data. Mycoscience. 2006;47(2):55-64
  20. 20. Bissett J, Gams W, Jaklitsch W, Samuels GJ. AcceptedTrichodermanames in the year 2015. IMA Fungus. 2015;6(2):263-295
  21. 21. Herrera-Estrella A, Chet I. The biological control agentTrichoderma– From fundamentals to applications. In: Arora DK, Dekker M, editors. Fungal Biotechnology in Agricultural, Food and Environmental Applications. 21st ed. New York, USA: CRC Press; 2004. pp. 147-156
  22. 22. Harman GE. Overview of mechanisms and uses ofTrichodermaspp. Phytopathology. 2006;96(2):190-194
  23. 23. Vinale F, Sivasithamparam K, Ghisalberti EL, Marra R, Woo SL, Lorito M.Trichoderma– Plant – Pathogen interactions. Soil Biology and Biochemistry. 2008;40(1):1-10
  24. 24. Strakowska J, Błaszczyk L, Chełkowski J. The significance of cellulolytic enzymes produced byTrichodermain opportunistic lifestyle of this fungus. Journal of Basic Microbiology. 2014;54(Suppl. 1):S2-S13. DOI: 10.1002/jobm.201300821
  25. 25. Haran S, Schickler H, Oppenheim A, Chet I. Differential expression ofTrichoderma harzianumchitinases during mycoparasitism. Phytopathology. 1996;86(9):980-985
  26. 26. Jeleń H, Błaszczyk L, Chełkowski J, Rogowicz K, Strakowska J. Formation of 6-n-pentyl-2H-pyran-2-one (6-PAP) and other volatiles by differentTrichodermaspecies. Mycological Progress. 2013;13(3):589-600. DOI: 10.1007/s11557-013-0942-2
  27. 27. Lorito M, Hayes CK, Di Pietro A, Woo SL, Harman GE. Purification, characterization, and synergistic activity of a glucan 1,3-b-glucosidase and an N-acetyl-b-glucosaminidase fromTrichoderma harzianum. Phytopathology. 1994;84(4):398-405
  28. 28. Thrane C, Tronsmo A, Jensen DF. Endo-1,3-β-glucanase and cellulose fromTrichoderma harzianum: Purification and partial characterization, induction of and biological activity against plant pathogenicPythiumspp. European Journal of Plant Pathology. 1997;103(4):331-344
  29. 29. Viterbo A, Harel M, Chet I. Isolation of two aspartyl proteases fromTrichoderma asperellumexpressed during colonization of cucumber roots. FEMS Microbiology Letters. 2004;238(1):151-158
  30. 30. Sharon E, Bar-Eyal M, Chet I, Herrera-Estrella A, Kleifeld O, Spiegel Y. Biological control of root-knot nematodeMeloidogyne javanicabyTrichoderma harzianum. Phytopathology. 2001;91(7):687-693
  31. 31. Enari TM, Niku-Paavola ML. Enzymatic hydrolysis of cellulose: Is the current theory of the mechanisms of hydrolysis valid? Critical Reviews in Biotechnology. 1987;5(1):67-87
  32. 32. Gams W, Bisset J. Morphology and identification ofTrichoderma. In: Harman GE, Kubicek CP, editors. Trichoderma and Gliocladium. London, UK: Taylor & Francis; 1998. pp. 3-34
  33. 33. Reino JL, Guerrero RF, Hernandez-Galan R, Collado IG. Secondary metabolites from species of the biocontrol agentTrichoderma. Phytochemistry Reviews. 2008;7(1):89-123
  34. 34. Blaszczyk LMSKS, Siwulski M, Sobieralski K, Lisiecka J, Jedryczka M.Trichodermaspp.–application and prospects for use in organic farming and industry. Journal of Plant Protection Research. 2014;54(4):309-317
  35. 35. Corley DG, Miller-Wideman M, Durley RC. Isolation and structure of harzianum a: A new trichothecene fromTrichoderma harzianum. Journal of Natural Products. 1994;57(3):422-425
  36. 36. Monte E. UnderstandingTrichoderma: Between biotechnology and microbial ecology. International Microbiology. 2001;4(1):1-4
  37. 37. Jeger MJ, Jeffries P, Elad Y, Xu XM. A generic theoretical model for biological control of foliar plant diseases. Journal of Theoretical Biology. 2009;256(2):201-214
  38. 38. Gupta VK, Misra AK. Efficacy of bioagents against fusarium wilt of guava. Journal of Mycology and Plant Pathology. 2009;39(1):101
  39. 39. Anonymous. DARE/ICAR Annual Report 2009-10. Crop Management. 2010. p. 48-63
  40. 40. Anonymous. DARE/ICAR Annual Report 2013-14. Crop Management. 2014; p. 56-67
  41. 41. Available from:https://icar.org.in/files/DARE-ICARAnnualReport%202016-17English.pdfpp. 70. [Accessed: February 6, 2022]
  42. 42. Available from:https://icar.org.in/files/DAREAnnual%20Report-2017-18_(English).pdf[Accessed: February 6, 2022]
  43. 43. Bubici G, Kaushal M, Prigigallo MI, Gómez-Lama Cabanás C, Mercado-Blanco J. Biological control agents against fusarium wilt of banana. Frontiers in Microbiology. 2019;10:616
  44. 44. Misra AK. Progressive steps in understanding and solving guava wilt-a national problem. Indian Phytopathology. 2017;70(1):1-11
  45. 45. Anonymous. The Planters’ Handbook. Digitised and Published by Tocklai Experimental Station. Jorhat – 785008, Assam: Tea Research Association; 2005. p. 131
  46. 46. Barthakur BK, Dutta, P. Disease management in tea. In: Tea Field Management. A Compilation of Lecture Notes. B. K. Goswami. Jorhat, Assam, India: Tea Research Association, Tocklai Experimental Station; 2011. pp. 182-188
  47. 47. Anonymous. DARE/ICAR Annual Report 2008-09. Crop Management. 2009; pp. 46-59
  48. 48. Kumhar KC, Azariah B, Mitali B, Priyanka B, Tanima D. Biological and chemical control offusarium solani, causing dieback disease of teaCamellia sinensis(L): Anin vitrostudy. International Journal of Current Microbiology and Applied Sciences. 2015;4(8):955-963
  49. 49. Kumhar KC, Babu A, Bordoloi M, Benarjee P, Rajbongshi H. Comparative bioefficacy of fungicides andTrichodermaspp. againstPestalotiopsis theae, causing grey blight in tea (camelliasp.): Anin vitrostudy. International Journal of Current Research in Biosciences and Plant Biology. 2016;3(4):20-27
  50. 50. Kumhar KC, Babu A, Arulmarianathan JP, Deka B, Bordoloi M, Rajbongshi H, et al. Role of beneficial fungi in managing diseases and insect pests of tea plantation. Egyptian Journal of Biological Pest Control. 2020;30(1):1-9

Written By

Kishor Chand Kumhar, Dalvinder Pal Singh and Anil Kumar

Submitted: January 22nd, 2022 Reviewed: February 16th, 2022 Published: April 5th, 2022