\r\n\r\nThe publication of this book was supported by the Secretariat of the Convention on Biological Diversity, United Nations\r\n\r\n',isbn:null,printIsbn:"978-953-51-0255-7",pdfIsbn:"978-953-51-4324-6",doi:"10.5772/1410",price:139,priceEur:155,priceUsd:179,slug:"tropical-forests",numberOfPages:402,isOpenForSubmission:!1,isInWos:1,isInBkci:!0,hash:"55286837c680e9be2bc357abf678212e",bookSignature:"Padmini Sudarshana, Madhugiri Nageswara-Rao and Jaya R. 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She was also involved in Biosafety & Regulation of transgenic research & Scientific/Community Outreach programs at Monsanto. During post-doctoral tenure at Indian Institute of Science, and University of Agricultural Sciences, she studied hormonal regulation in parasitic plants and genetic diversity in tropical forests respectively. She worked on post-harvest storage of fruits and vegetables for her doctoral thesis. She has to her credit several research articles, book chapters, popular articles and patents. She received “Above and Beyond” and “Genomics Team” awards for significant contributions to projects in Monsanto. She was recognized as ‘member-in-spotlight’ by Genome India International. Dr. Sudarshana obtained her M.Sc. and M.Phil. from Mysore University and Ph.D from Central Food & Technological Research Institute, India.",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"2",institution:null}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:{id:"120847",title:"Dr.",name:"Madhugiri",middleName:null,surname:"Nageswara-Rao",slug:"madhugiri-nageswara-rao",fullName:"Madhugiri Nageswara-Rao",profilePictureURL:"https://mts.intechopen.com/storage/users/120847/images/5440_n.jpg",biography:"Madhugiri Nageswara-Rao, Ph.D. works in the areas of plant breeding; genomics; bioenergy; genetic engineering; population, and eco-evolutionary genetics. He is the author of peer-reviewed research articles, book chapters, popular articles, has guest-edited special issues for journals, edited books and newsletters. He was Adjunct Faculty at Polk State College, USA. His work has been broadcasted on Fox News, USA. He was invited by CBC-Radio, Canada, to speak on air. He has served in the ‘Executive Committee’ of GII. He was recognized as ‘Young Scientists’ by Bioclues, in ‘Member-in-spotlight’ of GII and featured in ASPB-News. The University of Florida’s International Programs appraised his contribution in ‘International Focus’. He has peer-reviewed manuscripts for prominent international journals and grant proposals for international institutions. \nDr. Rao obtained his B.Sc., M.Sc. from Bangalore University and Ph.D. from FRI, India. He was featured as ‘Tomorrow’s Principal Investigators: Rising Young Investigators’ by Genome Technology, USA. He secured ‘Silver Award’ as a team member from American Museum of Natural History, USA. He was also selected for AAAS/Science Program for Excellence in Science. 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\n
1. Introduction
\n
Enterococci are Gram-positive, non-spore forming and facultative anaerobic cocci. They are indigenous flora of the intestinal tract, oral cavity and vagina in healthy persons. The genus comprises 54 species which are ubiquitously present in nature [1]. Enterococci have emerged as an important nosocomial pathogens second to Staphylococci which is the leading cause of nosocomial infections worldwide [2]. Enterococci are important nosocomial pathogens causing up to 10% of all infections in the hospitalized patients [3]. In these Enterococci infections approximately 60% of infections are caused by Enterococcus faecalis and Enterococcus faecium causes the remaining [4]. In the last decade both E. faecalis and E. faecium have emerged as important nosocomial pathogens. Other Enterococcal species causing nosocomial human infections are E. avium, E. gallinarum, E. casseliflavus, E. durans, E. raffinosus and E. mundtii. Majority of clinical isolates (63–81%) are identified as E. faecalis, around 13–23% as E. faecium and other enterococcal species comprise around 3–4% of the clinical isolates [5].
\n
\n
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2. Prevalence of vancomycin resistant Enterococci (VRE)
\n
Nosocomial infections particularly by vancomycin resistant Enterococci (VRE) have become a major problem since last few years though VRE are organisms of low virulence and pathogenicity. Nosocomial infections caused by VRE are highly prevalent in intensive care units of hospitals. These infections are particularly high in presence of underlying health factors like diabetes, liver transplantation, neutropenia, diabetes mellitus and renal dysfunction. Recently it has also been seen that VRE bloodstream infections have higher mortality rates as compared to vancomycin susceptible Enterococci (VSE) [6, 7]. Data from countries like Germany shows an increase of VRE from less than 5% in 2001 to 14.5% in 2013 mainly vancomycin resistant E. faecium [8]. In Europe of all the nosocomial infections reported 9.6% were of Enterococci [9]. In USA 3% of the nosocomial infections are due to VRE [10]. VRE nosocomial infections cause greater number of invasive treatment resulting in extended stay in hospital and cost [11]. Hospitals in some countries have now established VRE screening in high risk areas and isolation of patients to prevent spread of the resistant pathogen [12]. A study has shown the prevalence of VRE colonization in patients who had history of previous administration of antibiotics for more than 2 weeks were 10 times more likely of getting VRE colonization [13]. Other studies have also reported similar findings which show antibiotic exposure can cause colonization of VRE in hospital settings because of their resistance to commonly used antibiotics, virulence factors and ability to acquire genes [14].
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3. Genetic factors and antibiotic resistance in VRE
\n
The genes Van A, Van B, Van C, Van D and Van E are responsible for vancomycin resistance in Enterococci. Van M has been identified which is also an important vancomycin resistant determinant among different E. faecium lineages in hospitals in Shanghai, China [15]. Each Van operon has different ecological origin, Van A has originated from soil organisms, van B, Van G and Van D from gut microbiota [16]. Vancomycin resistance in Enterococci is of two types (a) Intrinsic resistance—Enterococci spp. like E. gallinarum and E. casseliflavus show an inherent low level resistance to Vancomycin. They have Van C genes that produce Vancomycin minimum inhibitory concentration (2–32 μg/ml) [17] A hospital wide outbreak of vancomycin resistant E. gallinarum has been reported in Colombia showing that uncommon species of Enterococci are capable of spreading in the hospital environment and producing nosocomial infections [18]. The second type is (b) acquired resistance—Enterococci species acquire resistance genes and become resistant to vancomycin. This is seen in E. faecium and E. faecalis and to some extent in E. raffinosus, E. avium, E. durance and other enterococcal species. The most common isolated Enterococci species which is VRE in hospital settings is E. faecium. It has been seen that E. faecium produces high vancomycin minimum inhibitory concentration (64–1000 μg/ml) [19]. There has been a significant increase in VRE prevalence. The emergence and rapid spread of VRE has led to the use of new antibiotics like linezolid, daptomycin and tigecycline. Linezolid is a oxazolidinone antibiotic. An oxazolidinone resistance gene optr A has been identified in E. faecalis and E. faecium isolates of human and animal origin [20] .Linezolid resistance is still less prevalent reported as 1.1 and 1.8% in E. faecium and E. faecalis isolates from 19 US hospitals [21]. Daptomycin resistance is more prevalent in E. faecium than E. faecalis isolates. Around 3.9 and 0.2% of E. faecium and E. faecalis isolates have been reported in various hospital settings [22]. Tigecycline is a semisynthetic derivative of tetracycline. Tigecycline resistance in E. faecium and E. faecalis is rare and reported as 0.3%. It is being used to treat bacteremia caused by MDR enterococci. The increased use of antibiotics in hospitals is causing gut dysbiosis and enterococci possess surviving ability take over the niche in the gastrointestinal tract and this could be the primary source of enterococcal infections [23].
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4. Lineages of nosocomial Enterococci
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The ability of E. faecium to exchange mobile genetic elements carrying antimicrobial resistance genes and virulence determinants has resulted in hospital adapted clones [24]. Esp was the first adaptive element found in hospital strains of E. faecium. The E. faecium esp. gene has been linked to biofilm formation, UTI and endocarditis [24]. New determinants have been now linked to hospital isolates of E. faecium. A genomic analysis study of E. faecium hospital strains identified gain and loss of gene clusters in clinical and non-clinical isolates of E. faecium [25]. Genomic studies of nosocomial E. faecium infection have confirmed the transmission of E. faecium Clad A115. Recently it has been seen a significant presence of hospital associated VRE fm lineages in the wastewater and need of controlling healthcare associated dissemination of VRE fm [26]. However studies on E. faecalis ecotypes have shown no appearance of distinct E. faecalis strains over a significant period of time. Virulence factors like antibiotic resistance and virulence genes, esp., capsule polysaccharide genes and genes determining gelatinase, aggregation factor, cytolysin and ace are identified in E. faecalis isolates [27]. The non-emergence of distinct ecotypes of E. faecalis and multiplicity of closely related ecotypes is not seen in E. faecalis as compared to E. faecium. A genomic analysis of 168 E. faecalis hospital isolates showed no genes and non-synonymous single nucleotide polymorphisms in the three lineages of hospital strains [28]. A recent study has also demonstrated that the acquisition of mobile genetic elements in E. faecalis V583, makes it unable to coexist with commensal enterococci in humans [29].
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5. Nosocomial infections by VRE
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Nosocomial infections by Enterococci are Urinary tract infection, endocarditis, bacteremia, catheter related infections, wound infections, intra- abdominal and pelvic infections and recently even oral infections have been reported.
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6. Urinary tract infection (UTI)
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Enterococci cause both uncomplicated and complicated health care associated UTI. E. faecalis. Vancomycin resistant E. faecalis and vancomycin resistant E. faecium have been mainly implicated in Enterococcal UTI. VRE is fast becoming a major cause of health care associated UTI. The treatment of UTI involves the use of broad spectrum antibiotics which is a major cause of resistant strains to vancomycin (VRE). The complications range from uncomplicated cystitis, pyelonephritis, perinephric abscess, and prostatitis. These organisms are responsible for nosocomial infection of urinary tract particularly in intensive care units (ICU). Enterococci have been particularly reported in catheter associated urinary tract infections, CAUTI (28.4%). Enterococci species are capable of producing biofilms, which are a population of cells attached irreversibly on various biotic and abiotic surfaces. CAUTI are associated with multispecies biofilms. Biofilms are difficult to remove and result in many chronic infections. Bacteria in biofilms colonize medical devices such as catheters, pacemakers, prosthetic heart valves and orthopedic appliances [30]. These multispecies biofilms have synergistic or antagonistic effects of interspecies interaction. Many studies have shown the association of biofilm producing enterococci and urinary catheter [31, 32]. Enterococci biofilms which are formed on catheter in CAUTI are resistant to immune clearance, urination force and even antibiotics. These enterococci utilize fibrinogen formed on catheter surface and form resistant biofilms. E. faecalis attachment in biofilm formation seen in vitro is partially inhibited by uropathogenic E. coli (UPEC) but biofilm formation by K. pneumoniae or UPEC are not affected by E. faecalis but E. faecalis increased E. coli biofilm mass accumulation and it has been seen that co-culture of an E. faecium probiotic strain with enteropathogenic E. coli increased the antibiotic sensitivity of E. coli to aminoglycosides, B-lactams and quinolones [33]. Biofilm formation confers the organism resistance to phagocytosis and antimicrobial agents. UTI by E. faecalis is mediated by virulence factors of the genes esp., srtC, ebp A, ebpC, ace, epaB, msrA, msr B, sigV, efbA, and grvR/etaR. E. faecium also displays similar genes related to virulence. Both E. faecalis and E. faecium isolated from nosocomial UTIs show kidney tropism. It is important to study factors in enterococcal causing pyelonephritis [33].
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7. Bacteremia
\n
There is a high prevalence of blood stream infections caused by Gram-positive bacteria and 45% are caused by Enterococci. Bacteremia is a common manifestation of vancomycin resistant Enterococci. Due to use of intravascular and urinary catheters these nosocomial infections are acquired. E. faecium in the blood stream is associated with increased mortality due to high levels of resistance. Risk factors identified with VRE bacteremia include intestinal colonization, long term antibiotic use, severity of illness, bone marrow transplant, hematologic malignancy, indwelling urinary catheters, corticosteroid treatment, chemotherapy and parenteral nutrition [34]. Studies have shown that bacteremia caused by vancomycin resistant Enterococci strains carry higher mortality rates (2.5-fold increase) as compared to bacteremia caused by vancomycin sensitive strains. In one such study the prognosis of VRE bacteremia was not much changed even with the availability of antimicrobial agents with greater potency. E. faecalis sigma factor Sig V that regulates gene expression in response to stress conditions has been implicated in enterococci survival and colonization in systemic infection. Absence of sig V in systemic infection in mice resulted in attenuation of bacterial translocation reducing colonization of kidney and liver. Virulence factors like Bgs A and Bgs B have also been implicated in colonization of endocarditic lesions and bacteremia. BgsA and Bgs B are now being used to treat enterococcal infections by using them as drug targets [35]. Similarly gene Asr has been implicated in E. faecium pathogenesis in systemic infections. Nosocomial enterococcal bacteremia have been associated with urinary catheters, intra-abdominal, burn wound, pelvic, biliary and bone sources. VRE bacteremia results in 2.5-fold increase in mortality as compared to vancomycin sensitive (VSE) bacteremia [18].
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8. Infective endocarditis
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Enterococci are the second most cause of infective endocarditis. Endocarditis caused by VRE faecalis causes GI or GU manipulation, damaged mitral or aortic valve infections, liver transplantation whereas VRE faecium endocarditis is associated with infection of tricuspid valve [36]. E. faecalis is also associated with community acquired endocarditis. Characteristic signs of infection include fever or a new murmur. Typical stigmata of endocarditis like petechiae, osler spots are rare and occur with sub-acute infections. Genitourinary infection or instrumentation often precedes the onset of enterococcal endocarditis. In published series of enterococcal endocarditis men often outnumber women and mostly it occurred in elderly individuals. In the current therapeutic regimes, the mortality rate of enterococcal endocarditis remains around 20%.
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9. Intra-abdominal and pelvic infections
\n
VRE has been isolated from intra-abdominal and pelvic infections. The usual infections include abscesses wounds or peritonitis. Often it is a part of polymicrobial infection with Gram negative or anaerobic organisms. Usually infecting strains originate from patients intestinal flora and cause intra-abdominal infection. Enterococci are able to cause monomicrobial peritonitis infections particularly in patients undergoing chronic peritoneal dialysis or liver cirrhosis.
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10. Gastrointestinal infections (GI)
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GI related enterococcal infections are opportunistic infections particularly occurring during colorectal surgery and colorectal cancer. Pre-colonization with VRE in patients can result in bacteremia following antibiotic induced disruption of gut microbiota [37]. Reg IIIy, a c type lectin is secreted by intestinal epithelial and paneth cells that removes Gram positive bacteria from the gut. Antibiotic treatment causes Reg IIIy down-regulation [38]. Therapeutic strategies have been devised to prevent intestinal colonization of resistant enterococci, introducing probiotic E. faecalis pheromone induced killing of drug resistant E. faecalis reactivating Reg IIIy introducing obligate anaerobic commensal bacteria containing Barnesiella species which prevents E. faecium gut colonization and bacteremia [39]. High collagenase producing E. faecalis strains have been found to be associated with colorectal anastomotic leak by activating tissue matrix metalloproteinase 9 that cleaves host extracellular matrix [40]. Enterococci produce menaquinone and extracellular superoxide in intestine. This results in high oxidative stress which is linked with colorectal cancer as high genomic instability of intestinal tumor cells as around 80% of colon cancers are caused due to genetic mutations.
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11. Central nervous system infections (CNS)
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Although CNS infections have been reported rarely with VRE but occur in elderly patients having underlying health issues like malignancies, pulmonary and cardiac complications [41]. In them VRE faecium is reported at 82% and less so of VRE faecalis. These infections present as fever, mental disorientation, focal CNS deficits and petechial rash. CSF investigations show pleocytosis, low glucose and increased protein levels.
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12. Skin and skin structure infections (SSSE)
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Enterococci are part of polymicrobial infections which are found to be associated with SSSE [42]. Enterococci are frequently isolated from diabetic foot ulcers and 2–5% of patients undergoing inpatient surgery. In studies using animal models it has been seen that E. faecalis capsular polysaccharide in SSSI predominantly is related to the persistence of the organism. A gene cpsI encodes the carbohydrate for capsular polysaccharide.
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13. Oral infections
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Enterococci are inhabitants of the oral cavity and as opportunistic pathogen cause oral diseases like caries, endodontic infections, periodontitis and peri-implantitis. In endodontic infections the failure of root canal treatment by endodontic infections is now well evidenced. Enterococci have high resistance to endodontic medicaments and forms resistant biofilms. This is implicated in root canal treatment failure [43, 44]. Enterococci prevalence is also seen in gingivitis and periodontitis (3.7–35%) [45]. Oral Enterococci constitute the highest percentage of virulent genes and ability to form resistant biofilms. The oral cavity may hence be an important reservoir of virulent antibiotic resistant enterococci strains. VRE colonization occurs mainly in GI tract, skin, genitourinary tract and oral cavity. Enterococci can persist from months to years. The hands of health care workers are the most common source of transmission in nosocomial infections [46]. The need of oral care is particularly important in nosocomial settings. The spread of the nosocomial VRE occurs and when the immunity is lowered VRE multiply to cause disease. Few studies have shown that antibiotic resistant enterococci is transmitted by food [47, 48, 49] but recently Vidana et al. [50] have said there is no food related transmission of enterococci. Enterococci are now showing a high degree of resistance to tetracycline, chloramphenicol, erythromycin besides vancomycin pose a threat for spread of nosocomial infection particularly in patients of ICUs and on mechanical ventilators [51]. Vancomycin resistance is an independent predictor for the overall increase of hospital costs for the patient but also for the individual hospital [52].
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14. Conclusion
\n
VRE and have now become an important nosocomial pathogen globally. VRE causes range of infections from UTI, bacteremia, infective endocarditis, intra-abdominal and pelvic infections, central nervous system infections and even oral infections. The ability of enterococci to form recalcitrant biofilms, colonize and express virulence factors, genome plasticity, resistant to antibiotics, survival ability makes it an important nosocomial pathogen to which new therapeutic strategies have to be devised for the treatment of VRE. A periodic surveillance of VRE in hospitals is essential for limiting the spread of antibiotic resistance. Future therapy should be targeted to prevent VRE colonization of patients with immunosuppression.
\n
\n\n',keywords:"Enterococci, vancomycin, resistance, nosocomial, infections",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/70494.pdf",chapterXML:"https://mts.intechopen.com/source/xml/70494.xml",downloadPdfUrl:"/chapter/pdf-download/70494",previewPdfUrl:"/chapter/pdf-preview/70494",totalDownloads:676,totalViews:0,totalCrossrefCites:2,totalDimensionsCites:6,totalAltmetricsMentions:0,impactScore:2,impactScorePercentile:81,impactScoreQuartile:4,hasAltmetrics:0,dateSubmitted:"March 26th 2019",dateReviewed:"November 19th 2019",datePrePublished:"December 16th 2019",datePublished:"December 9th 2020",dateFinished:"December 16th 2019",readingETA:"0",abstract:"Enterococci, particularly Enterococcus faecalis and Enterococcus faecium, are an important cause of nosocomial infections and have become a major issue worldwide. Nosocomial infections due to vancomycin resistant Enterococci (VRE) occur frequently. A significant increase in prevalence of VRE has been reported recently in many countries. Enterococci are second most frequent cause of nosocomial urinary tract infection, bacteremia and infective endocarditis. They are also related to etiology of intra-abdominal an pelvic infections, gastrointestinal infections and oral infections. The ability of Enterococci to survive in adverse conditions, presence of virulence factors and possession of intrinsic and acquired antibiotic resistance traits poses a therapeutic challenge. Due to high level of multidrug resistance in VRE, Enterococcus has become an important organism in health based settings.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/70494",risUrl:"/chapter/ris/70494",book:{id:"8133",slug:"pathogenic-bacteria"},signatures:"Sonia Bhonchal Bhardwaj",authors:[{id:"178566",title:"Dr.",name:"Sonia Bhonchal",middleName:null,surname:"Bhardwaj",fullName:"Sonia Bhonchal Bhardwaj",slug:"sonia-bhonchal-bhardwaj",email:"sbbhardwaj2002@yahoo.com",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178566/images/system/178566.jpeg",institution:{name:"Panjab University",institutionURL:null,country:{name:"India"}}}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Prevalence of vancomycin resistant Enterococci (VRE)",level:"1"},{id:"sec_3",title:"3. Genetic factors and antibiotic resistance in VRE",level:"1"},{id:"sec_4",title:"4. Lineages of nosocomial Enterococci",level:"1"},{id:"sec_5",title:"5. Nosocomial infections by VRE",level:"1"},{id:"sec_6",title:"6. Urinary tract infection (UTI)",level:"1"},{id:"sec_7",title:"7. Bacteremia",level:"1"},{id:"sec_8",title:"8. Infective endocarditis",level:"1"},{id:"sec_9",title:"9. Intra-abdominal and pelvic infections",level:"1"},{id:"sec_10",title:"10. Gastrointestinal infections (GI)",level:"1"},{id:"sec_11",title:"11. Central nervous system infections (CNS)",level:"1"},{id:"sec_12",title:"12. Skin and skin structure infections (SSSE)",level:"1"},{id:"sec_13",title:"13. Oral infections",level:"1"},{id:"sec_14",title:"14. Conclusion",level:"1"}],chapterReferences:[{id:"B1",body:'\nDe Perio MA, Yarnold PR, Warrent J, et al. Risk factors and outcomes associated with non Enterococcus faecalis, non E. faecium enterococcal bacteremia. Infection Control and Hospital Epidemiology. 2006;27(1):28-33\n'},{id:"B2",body:'\nSakka V, Tsiodras S, Galani L, et al. 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Collagen degradation and MMP9 activation by E. faecalis contribute to intestinal anastomatic leak. Science Translational Medicine. 2015;7:286ra68. DOI: 10.1126/scitraslmed.3010658. PMID: 25947163\n'},{id:"B41",body:'\nWang JS, Muzewich K, Edmond MB, Bearman G, Stevens MP. Central nervous system infections due to VRE: Case series and review of literature. International Journal of Infectious Diseases. 2014;25:26-31\n'},{id:"B42",body:'\nArias CA, Murray BE. Enterococcus species, Streptococccus gallolyticus group and Leuconostoc species. In: Bennett JE, Dolin R, Blaser MJ, editors. Marebel Douglas and Bennetts: Principles and Practice of Infectious Diseases. Philadelphia: Saunders; 2015. pp. 2328-2339\n'},{id:"B43",body:'\nDuggan JM, Sedgley CM. Biofilm formation of oral and endodontic E. faecalis. Journal of Endodontia. 2007;33:815-818. PMID: 17804318\n'},{id:"B44",body:'\nAppelbe OK, Sedgley CM. Effects of prolonged exposure to alkaline pH on E. faecalis survival and specific gene transcripts. Oral Microbiology and Immunology. 2007;22:169-174. PMID: 17488442\n\n'},{id:"B45",body:'\nSun J, Sundsford A, Song X. E. faecalis from patients with chronic periodontitis: virulence and antimicrobial resistance traits and determinants. European Journal of Clinical Microbiology & Infectious Diseases. 2012;31:267-272. DOI: 10.1007/s10096-011-1305-zPMID21660501\n'},{id:"B46",body:'\nSnyder GM, Thom KA, Faruno JP, et al. Detection of methicillin resistant Staphylococcus aureus and vancomycin resistant enterococci on the gowns and gloves of healthcare workers. Infection Control and Hospital Epidemiology. 2008;29(7):583-589\n'},{id:"B47",body:'\nAnderson AC, Jonas D, Huber I, Karygianni L, Nolber J, Hellwig E, et al. E. faecalis from food, clinical specimens and oral sites. Prevalence of virulence factors in association with biofilm formation. Frontiers in Microbiology. 2016;6:1534. DOI: 10.3389/fmcib.2015.01534. PMID: 01534; PMID: 26793174\n'},{id:"B48",body:'\nLinden PK. Optimising therapy for vancomycin resistant enterococci (VRE). Seminars in Respiratory and Critical Care Medicine. 2007;28(6):632-645\n'},{id:"B49",body:'\nMiranda JM, Franco CM, Vasquez BI, Fente CA, Barros-Velazquez J, Cepeda A. Evaluation of chromooccult enterococci agar for the isolation and selective enumeration of Enterococcus species in broilers. Letters in Applied Microbiology. 2005;41:153-156\n'},{id:"B50",body:'\nVidana R, Rashid MU, Ozenci V, Weintraub A, Lund B. The origin of E. faecalis explored by comparison of virulence factor patterns and antibiotic resistance to that of isolates from stool samples, blood cultures and food. International Endodontic Journal. 2016;49(4):343-351. DOI: 10.1111/iej.1264 PMID 25950381\n'},{id:"B51",body:'\nKomiyama EY, Lepesqueur LSS, Yassuda CG, Samaranayake LP, Parahitiyawa NB, Balducci I, et al. Enterococci species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility. PLoS One;11(9):e0163001. DOI: 10:1371/journal.Pone.0163001\n'},{id:"B52",body:'\nPuchter L, Chaberny IF, Schwab F, Vonberg RP, Bange FC, Ebadi E. Economic burden of nosocomial infections caused by vancomycin resistant enterococci. Antimicrobial Resistance and Infection Control. 2018;7:1. DOI: 10.1186/s13756-017-0291-z\n'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Sonia Bhonchal Bhardwaj",address:"sbbhardwaj2002@yahoo.com",affiliation:'
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1. Introduction
Vortices are common in fluid motion that originates due to the rotation of fluid elements. They occur widely and extensively in a broad range of physical systems from the earth’s surface to interstellar space. A few examples include spiral galaxies in the universe, red spots of Jupiter, tornadoes, hurricanes, airplane trailing vortices, swirling flows in turbines and in different industrial facilities, vortex rings formed by the firing of certain artillery or in the mushroom cloud resulting from a nuclear explosion. The physical quantity that characterizes the rotation of fluid elements is the vorticity ω = ∇ × u where u is the fluid velocity. Qualitatively, it can be said that in the region of vortex formation, the vorticity concentration is high compared with its surrounding fluid elements. Vortices formed behind obstacles to a fluid flow are also an interesting observation in various aspects of daily life. Study on the fluid flow around obstacles dates back to the fifteenth century when Leonardo da Vinci drew some sketches of vortex formation behind obstacles in flowing fluids. It has been an interesting and challenging problem in fluid mechanics and is of basic importance in several areas such as the study of aircraft designing, oceanography, atmospheric dynamics, engineering, human blood circulation. [1, 2, 3, 4]. Analyzing the behavior of flow around such obstacles also provides a medium to study the physical mechanism of transition from laminar to turbulent flow. If a stationary solid boundary lies in the path of a fluid flow, the fluid stops moving on that boundary. Thereby, a boundary layer is formed and its separation from the solid boundary generates various free shear layers that curl into concentrated vortices. These vortices then evolve, interact, become unstable and detach to turbulence. The dynamics of fluids is very diverse and the detail characteristics of transition to turbulence are quite complicated, which also differ from flow to flow. Such understandings can only be realized by experiments and computational models. However, there are a few unifying themes in the theory and a few routes to turbulence that are shared by many flows. One such theme is that when the Reynolds number (the parameter measuring the speed of a class of similar flows with steady configuration) increases, the temporal and spatial complexity of the flows increases eventually leading to turbulence. At a low Reynolds number, a pair of counter-rotating vortices forms behind the cylinder. As the Reynolds number increases, the vortices become unstable and gradually evolve into a von Karman vortex street [5, 6, 7, 8, 9]. The topic of flow past an obstacle is of utmost importance from the experimental point of view also. Its understanding is applicable in the stability of submerged structures, vortex-induced vibrations, etc. [10].
Vortices have been extensively studied and explored in the liquid state of matter. However, scientists have also extended their research to study the formation and behavior of vortices in the fourth state of matter, the plasma. Measurements done in space have shown that plasma vortices appear in the earth’s magnetosphere as well as along the Venus wake. On both planets, the solar wind encounters different obstacles. For earth, it is the earth’s magnetic field and for Venus, the interaction takes place with the ionized components in the upper layer of the planet’s atmosphere. Plasma vortices in earth-based laboratories have also been studied theoretically and experimentally [11, 12, 13, 14, 15]. Plasma is said to cover more than 99% of the matter found in the universe and dust particles are the unavoidable, omnipresent ingredients in it. Hence, in most cases, plasma and dust particles exist together, and these particles are massive (billion times heavier than the protons). Their size ranges from tens of nanometer to as large as hundreds of microns. Foreign particles in the plasma environment get charged up by the inflow of electrons and ions present in the plasma. The presence of these charged and massive particles increases the complexity of the plasma environment, and hence, this class of plasma has been named as ‘complex plasmas’ or ‘dusty plasmas’. They involve in a rich variety of physical and chemical processes and are thus investigated as a model system for various dynamical processes [16, 17]. Phase transition is an important and characteristic feature in dusty plasmas, due to which it is considered as a versatile medium to study all the three different phases (solid, liquid and gas) in just a single phase. They also behave as many particle interacting systems and provide a unique platform to study various organized collective effects prevalent in fluids, clusters, crystals, etc., in greater spatial and temporal resolution. With the help of laser light scattering, it is possible to visualize the micrometer or nanometer-sized dust particles through proper illumination. This allows to study the various phenomena in dusty plasma in greater spatial and temporal resolution since they appear in a slower time scale owing to their heavier mass [18, 19]. Along with a variety of dynamic phenomena that includes waves, shocks, solitons, etc., dusty plasma medium also supports the formation of vortices. Self-generated vortices have been observed in many dusty plasma experiments, which have been dealt with significant attention. The main cause of such vortex formation is the nonzero curl of the various forces acting on the electrically charged dust particles that are commonly found in radiofrequency (RF) discharges, microgravity conditions and subsonic dusty plasma flow with low Reynolds numbers [20, 21]. The nonzero component of the curl induces a rotational motion to the charged dust particles, which leads to the formation of the vortices. Depending on the different plasma production mechanisms and dust levitation (floating of dust particles in the plasma medium), the causes of the rotation of dust particles vary accordingly. Most importantly, the problem of fluid flow around obstacles can be investigated at the most elementary individual particle level in dusty plasmas. The existence of a liquid phase of dusty plasmas provides us the suitable conditions for the study. However, the obstacles used for such study in dusty plasmas are different from the solid obstacles in the hydrodynamic fluid medium.
In this chapter, we will concentrate mainly on dusty plasmas, their characteristics and a model system to study fluid flow around an obstacle at the particle level. After the introductory portion in Section 1, the fundamentals of dusty plasma are discussed in Section 2. In Section 3, the production of a dusty plasma medium by RF discharge will be discussed. Then in Section 4, we will discuss about the type and behavior of the obstacle which is used in dusty plasma flows. In Section 5, we will discuss the dusty plasma flow and the pattern formation behind the obstacle. The final section then summarizes the chapter as a whole.
2. Fundamentals of dusty plasma
2.1 Dusty plasma
First, let us start with a very brief explanation of plasma! Basically, plasma is an assembly of a nearly equal number of electrons and ions, and the charge neutrality is sustained on a macroscopic scale. In the absence of any external disturbance, that is, under equilibrium conditions, the resulting total electric charge is zero. The microscopic space-charge fields cancel out inside the plasma and the net charge over a macroscopic region vanishes totally. The quasi-neutrality condition at equilibrium is given by,
ne≈niE1
where ne and ni are the electron and ion densities, respectively.
The ‘plasma’ state of matter differs from ordinary fluids and solids by its natural property of exhibiting collective behavior. These collective effects result in the occurrence of various physical phenomena in the plasma, resulting in the long-range of electromagnetic forces among the charged particles. The very first example of plasma that is obvious to refer is the Sun, the source of existence of life. The protective layer to the earth’s atmosphere, known as the ionosphere, also remains in the form of ionized particles, that is plasma. Moreover, natural plasmas exist in interstellar space, stars, intergalactic space, galaxies, etc. On earth, the common form of natural plasma is lightning, fire and the amazing Aurora Borealis. Artificial plasmas are generated by applying electric or magnetic fields through a gas at low pressures. These are commonly found in street lights, neon lights, etc. Neon light is a gas discharge light, which is actually a sealed glass tube with metal electrodes at both the ends of the tube and filled with one or several gases at low pressure.
As already mentioned before, dust particles in space as well as in earth’s atmosphere, are unavoidable. These particles in plasma form a new field, that is dusty plasma or complex plasma. Dusty plasma is defined as a normal electron-ion plasma with charged dust components added to it. Naturally, dust grains are metallic, conducting, or made of ice particulates. Until and unless these are manufactured in laboratories, their shape and size vary. Depending on the surrounding plasma environments (due to the inflow of electrons and ions), dust particulates are either negatively or positively charged. These charged particles as a whole affect the plasma and result in collective and unusual behavior. When observed from afar, dust particles can be considered as point charges. As they are charged by the plasma species (electrons and ions), the charge neutrality condition is now modified, which is given by,
Qdnd0+eneo=enioE2
where ne0, ni0 and nd0 are the equilibrium densities of electrons, ions and dust, respectively, ‘e’ is the magnitude of electron charge, Qd=eZd is the charge on the dust’s surface and Zd is the dust charge number. It is important to highlight that the charge of the particles depends significantly on the plasma parameters. And the basic physics of the dusty plasma medium entirely rests on the Qdnd0 term of the charge neutrality condition.
Plasma possesses the fundamental property of shielding any external potential by forming a space charge around it. This particular property provides a measure of the distance over which the influence of the electric field of a charged particle (dust particle in our case) is experienced by other particles (electrons and ions) inside the plasma. Typically, this length is known as the dust Debye length λD, within which the dust particles can rearrange themselves to shield all the existing electrostatic fields. The negatively charged heavier dust particles are assumed to form a uniform background and the electrons and ions, which are assumed to be in thermal equilibrium, simply obey the Boltzmann distribution. The dust Debye length is given by,
λD=λDeλDiλDe2+λDi2E3
where λDe and λDi are electron and ion Debye lengths, respectively. These are expressed as,
λDe=kBTe4πneoe2E4
λDi=kBTi4πnioe2E5
Te,i represents the electron and ion temperatures, respectively, neo,io are the electron and ion densities, respectively, and kB is the Botlzmann constant.
A pictorial representation of a dusty plasma medium is shown in Figure 1.
Figure 1.
Schematic of a dusty plasma medium. The pink-shaded portion is the plasma medium. The green ball is the dust particle that is negatively charged. λD is the dust Debye length.
In a dusty plasma medium, the charged particles interact with each other via the electrostatic Coulomb force. However, due to the inherent shielding property of the plasma electrons and ions, the charged particles are shielded and hence, the interaction energy among them is known as Screened Coulomb or Yukawa potential energy. Consider two dust particles having the same charge Qd and separated by a distance ‘a’. The screened Coulomb potential energy is given by,
P.E=Qd24πϵ0ae−κE6
where κ=aλD is the screening strength. The dust thermal energy is given by,
K.E=kBTDE7
where TD is the dust temperature. The ratio of the P.E to the K.E is termed as the Coulomb Coupling parameter, given by
Γ=Qd24πϵ0akBTDe−κE8
Depending on the coupling parameter, a dusty plasma system remains in a weakly coupled state or a strongly coupled one. When Γ<1, the thermal energy of the dust particles is greater than the potential energy of the system and the system is said to be weakly coupled. On the other hand, when the potential energy exceeds the thermal energy, that is Γ>1 the system becomes strongly coupled. So, from Eq. (8), we can see that dust charge, screening parameter and the dust temperature play an important role in determining the system’s coupling state. As Γ exceeds a critical value Γc, called the critical coupling parameter, a dusty plasma system attains a crystalline state. However, this critical value for crystallization is dependent on the screening parameter [22]. For1<Γ<Γc, the system remains in a fluid (liquid or gas) state.
Thus, we see that by adjusting the dusty plasma parameters, we can obtain a fluid state of the dusty plasma medium experimentally. This provides us a unique model to study vortex formation behind an obstacle in the particle most level.
3. Production of a dusty plasma medium
Laboratory dusty plasmas differ from space and astrophysical dusty plasmas in a significant manner. The discharges done in the laboratory have geometrical boundaries. The composition, structure, conductivity, temperature, etc., of these geometries affect the formation and transport of the dust grains. Also, the external circuit, which produces and sustains the dusty plasma, imposes boundary conditions on the dusty discharge, which vary spatially as well as temporally. Dusty plasmas in the laboratory are generally produced by two main discharge techniques—direct current (DC) discharge and RF discharge. In this chapter, we will mainly focus on the production technique by RF discharge method in a DUPLEX device.
As the name suggests, DUPLEX is an abbreviation for Dusty Plasma Experimental Chamber. It comprises of a cylindrical glass chamber, 100 cm in length and 15 cm in diameter. The glass chamber configuration of the DUPLEX device provides a suitable and great access for optical diagnostics. One end of the cylindrical chamber is connected to the vacuum pump systems and the other end is closed by a stainless steel (SS) flange with Teflon O-ring between the glass chamber and the SS flange. On this closed end, there are ports for pressure gauge fitting, probe insertion and electrical connections. A radio frequency power generator (frequency: 13.56 MHz, power: 0–300 W) and an RF matching network are used for the plasma discharge. The RF antennas used in this setup are aluminum strips of 2.5 cm width and 20 cm length typically placed on the outer surface of the glass chamber. A schematic of the setup is shown in Figure 2. This strip acts as the live electrodes.
Figure 2.
Schematic of a DUPLEX setup. The pink-shaded portion is the argon plasma.
Initially, the chamber pressure is reduced to a value of about ∼10−3 mbar with the help of a rotary pump. Argon is used as the discharge gas, by injecting which the desired chamber pressure can be maintained. A grounded base plate is also inserted into the chamber (about ∼30 cm length, 14.5 cm width and 0.2 cm thickness), which acts as the grounded electrode and the region above it is selected as the experimental region. Applying a radiofrequency power (13.56 MHz and 5 W) between the aluminum strips (working as live electrodes) and the grounded base plate, a capacitively coupled RF discharge plasma is produced. Due to the application of the RF field, initially, the stray electrons inside the chamber get energized and in turn ionize the gas molecules present in the chamber. The aluminum strips used as live electrode outside give the flexibility to change the electrode position whenever required. Also, it facilitates in forming a uniform plasma over an extensive area of the grounded plate, that is the experimental region. The plasma parameters can be varied manually by tuning the discharge conditions, viz. RF power and neutral pressure.
Dust particles used in the experiment are gold-coated silica dust particles (∼ 5 micron diameter). These are initially put inside a buzzer that is fitted to the grounded base plate. After the production of the plasma, a direct current (DC) voltage of ∼ (6–12)V is applied to the buzzer, which ejects the dust particles from it through a hole. When these dust particles enter into the plasma environment, electrons and ions flow towards it and charge up the particles. In the laboratory, the dust particles are usually negatively charged as the electrons are lighter and highly mobile than the ions. These negatively charged dust particles are acted upon by two forces mainly, the upward electric field force (QdE) due to the sheath electric field (E) of the grounded base plate and the downward gravitational force (mdg). Dust particles levitate at the position where these two forces exactly balance. The dust particles are illuminated by laser light scattering, and the dust dynamics are recorded in high-speed cameras. Figure 3 shows the levitation of dust particles in a plasma medium. Above the dust layer, the purple color signifies argon discharge plasma. The dark region below the dust layer and above the plate is the sheath (where ionization does not take place) where a strong electric field (E) is present. The charged dust particles levitate at the interface region (∼ 0.8 cm above the plate) between plasma and the sheath where the force balance occurs. This is shown by a dashed line.
Figure 3.
Photograph of a dust layer levitation in plasma.
4. Obstacle in dusty plasma flows
The obstacle used in dusty plasma flow experiments is actually a dust void. A void is a dust-free region, which is encountered spontaneously in certain experimental conditions or can be produced externally also [23, 24, 25, 26, 27, 28]. In the past couple of decades, there have been a few studies on the interaction of a dusty plasma medium with dust voids. In 2004, Morfill et al. studied a laminar flow of liquid dusty plasma with a velocity ∼ 0.8 cms−1 around a spontaneously generated lentil-shaped void [29]. They observed the formation of a wake behind the void that is separated from the laminar flow region by a mixing layer. The flow also exhibited stable vortex flows adjacent to the boundary of the mixing layer. Another study was made in 2012 by Saitou et al. where they externally placed a thin conducting wire of 0.2 mm diameter and 2.5 cm length. They made the dust particles flow with velocity in the range ∼ (5–15) cms−1 but did not observe any vortex formation behind the obstacle. What they observed was a bow shock in front of it [30]. In the very next year itself (2013), Meyer et al. also did a similar experiment with a different configuration and dust flow mechanism (velocity ∼ 10–25 cms−1) than Saitou’s [21]. They produced a dust void by placing a 0.5-mm-diameter cylindrical wire transverse to the flow. They too observed a bow shock and a tear-shaped wake in front and behind the obstacle, respectively. Moreover, Charan et al. in 2016 did a molecular dynamics simulation study where they used a square obstacle and observed von Karman vortex street at low Reynolds number (i.e. low velocity) compared with normal hydrodynamic fluids [31]. Then in 2018, Jaiswal et al. investigated dust flow towards a spherical obstacle over a range of flow velocities ∼ (4–15)cms−1 and different obstacle biases [32]. The spherical obstacle also generated a dust-free area in its vicinity. They too observed bow shock formation in front of the obstacle but no vortex formation behind it. In 2020, Bailung et al. also investigated the study of dust flow around a dust void with a unique flow mechanism (dust flow velocity ∼ 3–10 cms−1) in a DUPLEX setup [33]. Dust particles are allowed to flow towards an already existing stationary dust layer. They could observe the formation of a counter-rotating pair of vortices behind the obstacle in a particularly narrow range of velocity ∼ (4–7) cms−1. Above and below this range, their vortices are not observed. Due to the interplay between these two forces, a circular void is generated around the pin. At the void boundary, these two forces equate with each other.
In the next section, we will study the results of Bailung et al. in detail, but before that let us understand the mechanism of the formation of dust void due to the insertion of an external cylindrical wire. A cylindrical pin inside the plasma attains a negative potential for the plasma and a sheath is formed in its vicinity. Due to the negative potential of the pin, ions try to drift towards it giving rise to a force on the dust particles named as ion drag force. This force is directed radially inward with the pin as the centre. Also, the negatively charged dust particles experience a repulsive electrostatic force from the pin which is directed radially outward. The interplay between these two forces generates a circular void around the pin. At the void boundary, these two forces equate with each other. A typical configuration of pin insertion through the grounded plate of a DUPLEX chamber is shown in Figure 4. The pin is externally connected to a DC bias voltage. By varying the bias voltage, the size of the void can be altered according to experimental requirements. Typically, at a RF power of 5 W and chamber pressure ∼ 0.02 mbar, the diameter of the dust void in floating condition (i.e. no external bias) is ∼1.7 cm. A typical example of a dust void is shown in Figure 5. However, unlike the solid obstacles in the case of hydrodynamic fluids, the dust void is not a rigid kind of obstacle. As already seen, the boundary of the void is maintained by a delicate force balance between the outward electrostatic force and inward ion drag force. An incoming dust flow, depending on the velocity of the flow, would cross the void boundary and penetrate into the void.
Figure 4.
A typical configuration for insertion of a pin through a grounded base plate in DUPLEX chamber.
Figure 5.
Snapshot of a dust void formed in DUPLEX chamber. The bright spot in the Centre is the reflection of laser light from the cylindrical pin. The photograph is taken from the top of the chamber.
5. Vortices in the wake of a dust void
Due to the non-rigidity of the dust void boundary, the behavior of the flow near the obstacle is somewhat different than conditions of hydrodynamic fluid with a rigid obstacle. Despite this difference, the transition from laminar to turbulence is observed in the wake of the obstacle in the case of dusty plasma flow also. As the flow approaches the void boundary, the middle section of the flow slightly penetrates into the void region and slips through the void boundary layer on both sides. The trajectory of the flow (in the mid-section) is deflected in front of the void due to the repulsive force exerted by the sheath electric field of the void and then flows downstream surrounding the void. The curved dust flow again meets behind the void and continues with the flow. As observed by Bailung et al. at a very narrow range of velocity ∼ (4–7) cms−1, a counter-rotating vortex pair is seen to appear. Below and above this range, the dust particles do not form any vortices. A typical example of three different conditions is shown in Figure 6.
Figure 6.
Typical snapshots showing structures formed behind the void at (a) 3.5cms−1, (b) 4.5cms−1, (c) 8 cm−1.
In each of the images, dust particles flow from right to left shown by dashed arrows. The top image (a) depicts a flow with dust flow velocity ∼ 3.5 cms−1 and the snapshot is at time t = 1370 ms from a reference time (t = 0, when dust flow reaches the right edge of the images). The middle image (b) shows dust fluid flow velocity ∼ 4.5 cms−1 at t = 1033 ms showing a vortex pair formation behind the void. The vortices are shown by the two arrow marks. It is observed that vortices are not formed for larger flow velocity ∼ 8 cms−1 (image (c)). For such high velocities, flow trajectories behind the void are elongated and dynamics in the wake is rather complex due to cross-flow at high speed. The bright illuminated point at the centre of each image is the reflection of laser light from the pin. Two horizontal lines that appear in all the images are due to laser reflection from the wall of the glass chamber. It is noted that dust flow with unsteady laminar velocity, which is (4–7)cms−1, and optimum dust density in the experimental region above the grounded plate is required to generate the vortex behind the void.
For a better understanding, a pictorial representation showing the dusty plasma streamlines around dust void at three different velocities are shown in Figure 7(a)–(c) of Figure 7 corresponds to the observation shown in (a), (b) and (c) of Figure 6.
Figure 7.
A pictorial illustration of the dusty plasma flow interaction with the dust void at different flow velocities. (a) Laminar flow, (b) unsteady laminar flow with filamentary vortex-type structure in the upstream and vortex pair in the downstream and (c) turbulent flow.
At a lower dust flow velocity, the void in the upstream is slightly compressed and trajectories of the streamlines flowing close to the void (boundary layer) curl behind the void. However, no structure formation in the wake appears here. Dust particles, after meeting behind the void, just continue with the flow smoothly. For critical flow speed (b), flow dynamics in the upstream void boundary is quite different. Streamlines that hit perpendicularly at the void flow some distance into the void region. They reconstruct the boundary during the flow and get ejected backward making the streamline bifurcation to occur much ahead of the void boundary. The curved streamlines, which are ejected backward, again flow along with the incoming dust flow close to the boundary layer. This critical reorientation in the front of the void generates a suitable condition for the formation of the vortex pair behind the void. Particles get slowed down in this region and these slower particles flow close to the boundary layer around the void and contribute in the formation of the vortex pair. At higher velocities (c), that is above the critical range for vortex formation, all the particles that hit the upstream void boundary are flushed away by the flow along with it. The streamlines intersect and crossover at a distance far behind the void and there is no formation of any stable structure. It is well known that in hydrodynamic fluids, at much higher velocities, vortex streets are observed. However, here such streets are not observed to form. This may be due to the restriction of the experimental geometry. The transition from laminar to turbulence is well known in fluid dynamics. But studying it in dusty plasma provides the chance to observe the individual particle-level trajectory. In turn, the dynamics can be studied in greater detail.
To see the dust dynamics in greater detail, let us look at the vortex formation behind the dust void step by step. At the outset of the formation, the slower particles moving along the curved boundary layer interact with the stationary particles behind the void and start to swirl on each side. The flow front then meets in the wake region behind the void (Figure 8(a)) and gradually traverses a swirling circular path. This is evident in the dotted arrow marks in (Figure b). After duration of 966 ms from the start of the flow, two counter-rotating vortices complete their formation (Figure c). Only the slower particles flowing close to the boundary layer participate in this swirling motion due to the nonzero curl of the forces. Those particles away from the boundary layer move faster and do not contribute to the swirling. With increasing time and inflow of more particles, the swirling finally grows into a distinct pair of the vortex with an eye in the middle (Figure (d)). As the flow progresses by maintaining a constant inflow of particle flux, the vortex pair sustains till 1167 ms. The one shot of dust flow in the experiment done by Bailung et al. lasted for about 2 sec.
Figure 8.
The parallel arrows depict the direction of the dust flow. (a) when both the oppositely curling flow front meet behind the void. Dotted curve traces in (b) indicate flow trajectories. The arrows in (c) - (g) show the vortex pair. the vortices vanish with time when flow is nearly over (h).
Hence, gradually when the particle flux started decreasing, the vortex pair starts to die out. It is faintly visible till 1233 ms (Figure g). The time for the growth of the vortex pair is ∼200 ms (from the time the particles meet behind the void) and survives for duration of 200 ms (depending on the duration of accelerated dusty plasma fluid flow). Finally, they disappear after 1300 ms. The rotational frequency measured for the vortices is about ∼3 Hz.
It is already mentioned that the advantage of studying vortex dynamics in dusty plasma lies in the fact that particles can be individually tracked. Different particle tracking software and computational models are available, which can generate the velocity vectors of the trajectory of the particles and hence can give a quantitative interpretation of the experimentally observed results. One such particle tracking platform is OpenPIV (Open Particle Image Velocimetry) in MATLAB [34]. This helps to study the evolution of the vortex pair along with its vorticity. But to perform successful PIV from images, the recorded videos of the dust flow dynamics should have a high-quality resolution and must be in high speed. A PIV analysis performed on a video recorded at 100 frames per second is shown in Figure 9.
Figure 9.
PIV analysis showing the time evolution of the vortices for a duration of 1 sec. The velocity vectors and vorticity profile drawn from (a) (0.53-0.62) sec (b) (0.63-0.72) sec (c) (0.73-0.82) sec (d) (0.83-0.92) sec (e) (0.93-1.02) sec (f) (1.03-1.12) sec (g) (1.13-1.22) sec (h) (1.23-1.32) sec (i) (1.33-1.42) sec (j) (1.43-1.52) sec are shown. The color bar shows the value of vorticity in 1/s. The dotted circle in (a) shows the original position of the void boundary before the flow and the dot at the center of the circle depicts the pin position.
Each image in the figure is an average PIV result of 10 consecutive image frames. The position of the void and the pin position are drawn by a red-dashed circle and a red dot, respectively. The velocity vectors show the trajectory of the dust particles and the color code gives the value of the vorticity at different times in units of s−1. The slowing down of the particles in front of the void is clearly seen by comparing the velocity vectors’ lengths in Figure 9(a) and (b). The backflow of the incoming dust particles mentioned earlier (due to repulsive sheath electric field force of the pin) is also observed in (b). The curling of dust particles leading to vortex formation is evident from (c) and (d). The vorticity of the fully formed vortex pair is about ∼ (20–25)s−1, which is shown by the color bar in (e) and (f)). This is nearly equal to twice the measured angular frequency. With the decrease of the dust flow influx, the vortex structure deforms (vorticity ∼15 s−1) and breaks away into smaller vortices (vorticity ∼10s−1) as seen from (g) to (i). Vortices finally disappear in (j), evident from the vorticity value which almost tends to 0.
Reynolds number is the characteristic parameter that helps to predict flow patterns. It is the ratio of the inertial forces to the viscous forces and is given by,
Re=ρvdLηE9
where ρ is mass density, vd is dust velocity, L is the obstacle dimension, that is the void diameter and η is the viscosity of the dust fluid.
In case of dusty plasma fluids, the viscosity is estimated from the formula,
η=3η̂mdndωEa2E10
where η̂ is the normalized shear viscosity, md is the dust mass, nd is the dust density, ωE=ωpd/3 is the Einstein frequency, ωpd is the dust plasma frequency and a is the interparticle distance. The normalized shear viscosity in dusty plasma fluid is a function of the Coulomb coupling parameter Γ, which has been estimated for a range of coupling parameters in different conditions via simulation [35, 36]. For typical plasma parameters of DUPLEX chamber, that is,
md=1.7×10−13kg
nd=9×109m−3
ωpd=247.5s−1
a=3×10−4m
The viscosity is calculated to be 9×10−9 Pas.
Thus, the Reynolds number for dust flow velocity ∼ (3–10) cms−1 is estimated to be lying in the range 50–190. The vortex pair formation appears in a critical range of 60–90.
In the case of hydrodynamic fluid, the range of Reynolds number for vortex formation is 5–40, which is much lower compared with that in dusty plasma fluid. This is because the ratio ρ/η (which is the kinematic viscosity) is one order larger in the case of dusty plasma fluids than that in hydrodynamic fluids. The estimated kinematic viscosity for dusty plasma fluids is ∼0.088 cm2s−1, whereas the kinematic viscosity for water is ∼0.008 cm2s−1.
6. Conclusion
The study of vortices in the problem of flow past an obstacle is significant as it provides a platform to investigate the transition from laminar to turbulence. Formation of vortices in the wake region behind an obstacle appears in the unsteady laminar regime of flows and has been widely studied in hydrodynamic fluids. However, dusty plasma medium, which is a component of the fourth state of matter, provides a unique stage to study such phenomena at the particle level. A special property of this medium is that it can remain in both fluids (liquid- or gas-like) as well as the crystalline state. By mere adjustment of plasma conditions, the desired state can be obtained. The individual tracking of micron-sized dust particles by methods such as PIV (Particle Image Velocimetry) yields the particle trajectory in form of velocity vector fields. This gives a very clear picture of the behavior of flow near obstacle boundaries. However, the obstacles used in dusty plasma flow experiments differ from those in hydrodynamic fluid experiments in the sense that unlike those in hydrodynamics, the obstacle boundaries in dusty plasma are non-rigid. Any foreign pin or wire inserted into the plasma would possess a negative potential with respect to the plasma. Dust particles in its vicinity are repelled due to electrostatic force and form a dust-free region around it, called the dust void. This dust void, whose boundary is delicately maintained by dusty plasma forces, acts as a non-rigid type of obstacle. Dusty plasma flows also generate counter-rotating vortices in the wake region behind a dust void at a particular range of velocities. Below and above this range, no structure formation is seen to appear. The particle behavior causing the formation of the vortices is better understood by tracking particles in consecutive frames. The estimated Reynolds number value for vortices to appear in the wake of a void in a dusty plasma medium is estimated to lie in the range 60–90. This is quite larger than the Reynolds number range for hydrodynamic fluids which is roughly about 5–40. This higher range in dusty plasma medium is attributed to the higher kinematic viscosity of dusty plasma fluids. However, in dusty plasma experiments, Von Karman vortex streets (observed in the turbulent regime of hydrodynamic fluids) are not yet explored. If such experiments could be successfully performed, then there will be immense scope of understanding turbulence at the particle-most level and with a better perspective. Although to study turbulent dynamics, high-speed cameras with high-quality resolution would be necessary.
\n',keywords:"vortices, vorticity, fluid flow, Reynolds number, plasma, dusty plasma, obstacle, dust void, viscosity",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/79796.pdf",chapterXML:"https://mts.intechopen.com/source/xml/79796.xml",downloadPdfUrl:"/chapter/pdf-download/79796",previewPdfUrl:"/chapter/pdf-preview/79796",totalDownloads:27,totalViews:0,totalCrossrefCites:0,dateSubmitted:"September 30th 2021",dateReviewed:"November 8th 2021",datePrePublished:"March 29th 2022",datePublished:null,dateFinished:"December 24th 2021",readingETA:"0",abstract:"The beauty in the formation of vortices during flow around obstacles in fluid mechanics has fascinated mankind since ages. To beat the curiosity behind such an interesting phenomenon, researchers have been constantly investigating the underlying physics and its application in various areas of science. Examining the behavior of the flow and pattern formations behind an obstacle renders a suitable platform to realize the transition from laminar to turbulence. A dusty plasma system comprising of micron-sized particles acts as a unique and versatile medium to investigate such flow behavior at the most kinetic level. In this perspective, this chapter provides a brief discussion on the fundamentals of dusty plasma and its characteristics. Adding to this, a discussion on the generation of a dusty plasma medium is provided. Then, a unique model of inducing a dusty plasma flow past an obstacle at different velocities, producing counter-rotating symmetric vortices, is discussed. The obstacle in the experiment is a dust void, which is a static structure in a dusty plasma medium. Its generation mechanism is also discussed in the chapter.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/79796",risUrl:"/chapter/ris/79796",signatures:"Yoshiko Bailung and Heremba Bailung",book:{id:"10958",type:"book",title:"Vortex Dynamics - From Physical to Mathematical Aspects",subtitle:null,fullTitle:"Vortex Dynamics - From Physical to Mathematical Aspects",slug:null,publishedDate:null,bookSignature:"Prof. İlkay Bakırtaş and Dr. Nalan Antar",coverURL:"https://cdn.intechopen.com/books/images_new/10958.jpg",licenceType:"CC BY 3.0",editedByType:null,isbn:"978-1-80355-025-1",printIsbn:"978-1-80355-024-4",pdfIsbn:"978-1-80355-026-8",isAvailableForWebshopOrdering:!0,editors:[{id:"186388",title:"Prof.",name:"İlkay",middleName:null,surname:"Bakırtaş",slug:"ilkay-bakirtas",fullName:"İlkay Bakırtaş"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:null,sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Fundamentals of dusty plasma",level:"1"},{id:"sec_2_2",title:"2.1 Dusty plasma",level:"2"},{id:"sec_4",title:"3. Production of a dusty plasma medium",level:"1"},{id:"sec_5",title:"4. Obstacle in dusty plasma flows",level:"1"},{id:"sec_6",title:"5. Vortices in the wake of a dust void",level:"1"},{id:"sec_7",title:"6. Conclusion",level:"1"}],chapterReferences:[{id:"B1",body:'Lopez HM, Hulin JP, Auradou H, D’Angelo MV. Deformation of a flexible fiber in a viscous flow past an obstacle. Physics of Fluids. 2015;27:013102:1-12'},{id:"B2",body:'Saffman PG, Schatzman JC. Stability of a vortex street of finite vortices. Journal of Fluid Mechanics. 1982;117:171-185'},{id:"B3",body:'Zhang HQ, Fey U, Noack BR, Knig M, Eckelmann H. On the transition of the cylinder wake. Physics of Fluids. 1995;7:779-794'},{id:"B4",body:'Vasconcelos GL, Moura M. Vortex motion around a circular cylinder above a plane. Physics of Fluids. 2017;29:083603:1-8'},{id:"B5",body:'Votyakov EV, Kassinos SC. On the analogy between streamlined magnetic and solid obstacles. Physics of Fluids. 2009;21:097102:1-11'},{id:"B6",body:'Chenand D, Jirka GH. Experimental study of plane turbulent wakes in a shallow water layer. Fluid Dynamics Research. 1995;16:11-41'},{id:"B7",body:'Schar C, Smith RB. Shallow water flow past topography. Part II: Transition to vortex Shedding. Journal of the Atmospheric Sciences. 1993;50:1401-1412'},{id:"B8",body:'Balachandar R, Ramachandran S, Tachie MF. Characteristics of shallow turbulent near wakes at low Reynolds number. Journal of Fluids Engineering. 2000;122:302-308'},{id:"B9",body:'Koochesfahani MM. Vortical patterns in the wake of an oscillating airfoil. AIAA Journal. 1989;27:1200-1205'},{id:"B10",body:'Bharuthram R, Yu MY. Vortices in an anisotropic plasma. Physics Letters A. 1987;122:488-491'},{id:"B11",body:'Kervalishvili NA. Electron vortices in a nonneutral plasma in crossed E⊥H fields. Physics Letters A. 1991;157:391-394'},{id:"B12",body:'Kaladze TD, Shukla PK. Self-organization of electromagnetic waves into vortices in a magnetized electron-positron plasma. Astrophysics and Space Science. 1987;137:293-296'},{id:"B13",body:'Siddiqui H, Shah HA, Tsintsadze NL. Effect of trapping on vortices in plasma. Journal of Fusion Energy. 2008;27:216-224'},{id:"B14",body:'Mofiz UA. Electrostatic drift vortices in a hot rotating strongly magnetized electron-positron pulsar plasma. Astrophysics and Space Science. 1992;196:101-107'},{id:"B15",body:'Horton W, Liu J, Meiss JD, Sedlak JE. Solitary vortices in a rotating plasma. Physics of Fluids. 1986;29:1004-1010'},{id:"B16",body:'Morfill GE, Ivlev AV. Complex plasmas: An interdisciplinary research field. Reviews of Modern Physics. 2009;81:1353-1404'},{id:"B17",body:'Shukla PK. A survey of dusty plasma physics. Physics of Plasmas. 2001;8:1791-1803'},{id:"B18",body:'Burlaga LF. A heliospheric vortex street. Journal of Geophysical Research. 1990;95:4333-4336'},{id:"B19",body:'Hones EW, Birn J, Bame SJ, Asbridge JR, Paschmann G, Sckopke N, et al. Further determination of the characteristics of magnetospheric plasma vortices with Isee 1 and 2. Journal of Geophysical Research. 1981;86:814-820'},{id:"B20",body:'Schwabe M, Zhdanov S, Rath C, Graves DB, Thomas HM, Morfill GE. Collective effects in vortex movements in complex plasmas. Physical Review Letters. 2014;112:115002:1-5'},{id:"B21",body:'Meyer JK, Heinrich JR, Kim SH, Merlino RL. Interaction of a biased cylinder with a flowing dusty plasma. Journal of Plasma Physics. 2013;79:677-682'},{id:"B22",body:'Ichimaru S. Strongly coupled plasmas: High-density classical plasmas and degenerate electron liquids. Reviews of Modern Physics. 1982;54:1017'},{id:"B23",body:'Praburam G, Goree J. Experimental observation of very low-frequency macroscopic modes in a dusty plasma. Physics of Plasmas. 1996;3:1212-1219'},{id:"B24",body:'Samsonov D, Goree J. Instabilities in a dusty plasma with ion drag and ionization. Physical Review E. 1999;59:1047-1058'},{id:"B25",body:'Morfill GE, Thomas H, Konopka U, Rothermel H, Zuzic M, Ivlev A, et al. Condensed plasmas under microgravity. Physical Review Letters. 1999;83:1598-1601'},{id:"B26",body:'Rothermel H, Hagl T, Morfill GE, Thoma MH, Thomas HM. Gravity compensation in complex plasmas by application of a temperature gradient. Physical Review Letters. 2002;89:175001:1-4'},{id:"B27",body:'Fedoseev AV, Sukhinin GI, Dosbolayev MK, Ramazanov TS. Dust-void formation in a dc glow discharge. Physical Review E. 2015;92:023106:1-9'},{id:"B28",body:'Bailung Y, Deka T, Boruah A, Sharma SK, Pal AR, Chutia J, et al. Characteristics of dust voids in a strongly coupled laboratory dusty plasma. Physics of Plasmas. 2018;25:053705:1-8'},{id:"B29",body:'Morfill GE, Zuzic MR, Rothermel H, Ivlev AV, Klumov BA, Thomas HM, et al. Highly resolved fluid flows: “Liquid plasmas” at the kinetic level. Physical Review Letters. 2004;92:175004:1-4'},{id:"B30",body:'Saitou Y, Nakamura Y, Kamimura T, Ishihara O. Bow shock formation in a complex plasma. Physical Review Letters. 2012;108:065004:1-4'},{id:"B31",body:'Charan H, Ganesh R. Molecular dynamics study of flow past an obstacle in strongly coupled Yukawa liquid. Physics of Plasmas. 2016;23:123703:1-7'},{id:"B32",body:'Jaiswal S, Schwabe M, Sen A, Bandyopadhyay P. Experimental investigation of dynamical structures formed due to a complex plasma flowing past an obstacle. Physics of Plasmas. 2018;25:093703:1-10'},{id:"B33",body:'Bailung Y, Chutia B, Deka T, Boruah A, Sharma SK, Kumar S, et al. Vortex formation in a strongly coupled dusty plasma flow past an obstacle. Physics of Plasmas. 2020;27:123702:1-7'},{id:"B34",body:'Taylor ZJ, Gurka R, Kopp GA, Liberzon A. Long-duration time-resolved PIV to study unsteady aerodynamics. IEEE Transactions on Instrumentation and Measurement. 2010;59:3262-3269'},{id:"B35",body:'Saigo T, Hamaguchi S. Shear viscosity of strongly coupled Yukawa systems. Physics of Plasmas. 2002;9:1210-1216'},{id:"B36",body:'Donko Z, Goree J, Hartmann P, Kutasi K. Shear viscosity and Shear thinning in two-dimensional Yukawa liquids. Physical Review Letters. 2006;96:145003:1-4'}],footnotes:[],contributors:[{corresp:null,contributorFullName:"Yoshiko Bailung",address:null,affiliation:'
Dusty Plasma Laboratory, Physical Sciences Division Institute of Advanced Study in Science and Technology, India
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Assael AB, DMD, FACD, FICD is Professor and Chairman of Oral and Maxillofacial Surgery, Residency Program Director, and Medical Director Hospital Dentistry and Professor of Surgery OHSU Schools of Medicine and Dentistry in Portland, Oregon. Dr. Assael is an alumnus of Columbia University (AB in government), Harvard University (DMD), Vanderbilt University (residency in OMS) and the University of Kentucky (CMM). He is certified by the American Board of Oral and Maxillofacial Surgery. He served as Dean of the University Of Kentucky College Of Dentistry from 1997-2003. He was also on the faculty of the University of Connecticut from 1989-1997 and served as department head in surgical sciences and Associate Chief of Staff of the John Dempsey Hospital. From 1981-1989, he was residency program director in oral and maxillofacial surgery at the Mount Sinai School of Medicine. Dr. Assael is the past Editor in Chief of the Journal of Oral and Maxillofacial Surgery. He is the past Chairman of the Council on Dental Education and Licensure of the American Dental Association. He is the recipient of several national and international awards including The Donald Osbon award for outstanding educator, the William F. Harrigan award, and the Kurt Thoma award. He has also delivered lectureships honoring leaders in oral and maxillofacial surgery including William Ware, Sadie Fontaine, Peter Connole, Ben Alley, and Daniel Waite. In 2001, President George W. Bush awarded Dr. Assael the America’s Promise award for advancements in oral health in Appalachia achieved by the faculty of the University of Kentucky. In 2010, the American Association of Oral and Maxillofacial Surgeons awarded Dr. Assael the Board of Trustees special recognition award. The author of textbooks including books on temporomandibular disorders, cleft lip and palate and facial injuries, and over 100 peer reviewed contributions to the scientific literature, Dr. Assael’s main interests are listed below\n\n\nKey word areas of clinical research expertise\n\nAesthetic Surgery\nBallistic injuries\nBiomechanics\nBisphosphonates\nBone\nCraniomaxillofacial Trauma\nCraniomaxillofacial Reconstruction\nCleft Lip and Palate\nClinical Research design\nConnective tissue diseases\nEndoscopy\nFacial Pain\nHead and neck infection\nJaw tumors\nMedical economics\nMedical Editing, Peer review\nMedical informatics\nMedical Writing\nMicro-neurosurgery maxillofacial region\nMinimally invasive surgery\nObstructive Sleep Apnea\nOncology\nOral and maxillofacial pathology\nOral and maxillofacial surgery\nOrthognathic surgery\nOsteonecrosis\nOsteoporosis\nOsteoradionecrosis\nSalivary gland disease\nSurgical education\nTemporomandibular Disorders",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/100926/images/3556_n.jpg",totalCites:0,totalChapterViews:"0",outsideEditionCount:0,totalAuthoredChapters:"0",totalEditedBooks:"1",personalWebsiteURL:null,twitterURL:null,linkedinURL:null,institution:{name:"Oregon Health & Science University",institutionURL:null,country:{name:"United States of America"}}},booksEdited:[{id:"1170",type:"book",slug:"maxillofacial-surgery",title:"Maxillofacial Surgery",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/1170.jpg",abstract:"Oral and maxillofacial surgery is a specialty rooted in dentistry and forged in academic medical centers in departments of surgery, as the surgical specialty well equipped to care for conditions of the mouth, jaws, head and neck. Today oral and maxillofacial surgeons are advancing cancer care, neurosciences, understanding the pathology of the region, managing congenital and acquired deformities among others. In the process this specialty is improving the lives of our patients with better function, appearance, self esteem and longevity.",editors:[{id:"100926",title:"Prof.",name:"Leon",surname:"Assael",slug:"leon-assael",fullName:"Leon Assael"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",title:"Edited Volume"}}],chaptersAuthored:[],collaborators:[]},generic:{page:{slug:"open-access-funding-institutions-list",title:"List of Institutions by Country",intro:"
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2022",numberOfDownloads:3444,editors:[{id:"197720",title:"Ph.D.",name:"Kassio",middleName:null,surname:"Ferreira Mendes",slug:"kassio-ferreira-mendes",fullName:"Kassio Ferreira Mendes"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}}],latestBooks:[{type:"book",id:"10522",title:"Coding Theory",subtitle:"Recent Advances, New Perspectives and Applications",isOpenForSubmission:!1,hash:"6357e1dd7d38adeb519ca7a10dc9e5a0",slug:"coding-theory-recent-advances-new-perspectives-and-applications",bookSignature:"Sudhakar Radhakrishnan and Sudev Naduvath",coverURL:"https://cdn.intechopen.com/books/images_new/10522.jpg",editedByType:"Edited by",publishedDate:"May 25th 2022",editors:[{id:"26327",title:"Dr.",name:"Sudhakar",middleName:null,surname:"Radhakrishnan",slug:"sudhakar-radhakrishnan",fullName:"Sudhakar 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2022",editors:[{id:"90846",title:"Prof.",name:"Yusuf",middleName:null,surname:"Bozkurt",slug:"yusuf-bozkurt",fullName:"Yusuf Bozkurt"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10940",title:"Plant Hormones",subtitle:"Recent Advances, New Perspectives and Applications",isOpenForSubmission:!1,hash:"5aae8a345f8047ed528914ff3491f643",slug:"plant-hormones-recent-advances-new-perspectives-and-applications",bookSignature:"Christophe Hano",coverURL:"https://cdn.intechopen.com/books/images_new/10940.jpg",editedByType:"Edited by",publishedDate:"May 25th 2022",editors:[{id:"313856",title:"Dr.",name:"Christophe",middleName:"F.E.",surname:"Hano",slug:"christophe-hano",fullName:"Christophe Hano"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10207",title:"Sexual 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Science",slug:"surface-science"},numberOfBooks:5,numberOfSeries:0,numberOfAuthorsAndEditors:104,numberOfWosCitations:222,numberOfCrossrefCitations:123,numberOfDimensionsCitations:295,videoUrl:null,fallbackUrl:null,description:null},booksByTopicFilter:{topicId:"961",sort:"-publishedDate",limit:12,offset:0},booksByTopicCollection:[{type:"book",id:"7385",title:"Cavitation",subtitle:"Selected Issues",isOpenForSubmission:!1,hash:"075ee4bb432760777ffcba092d0cffae",slug:"cavitation-selected-issues",bookSignature:"Wojciech Borek, Tomasz Tański and Mariusz Król",coverURL:"https://cdn.intechopen.com/books/images_new/7385.jpg",editedByType:"Edited by",editors:[{id:"186373",title:"Dr.",name:"Wojciech",middleName:null,surname:"Borek",slug:"wojciech-borek",fullName:"Wojciech Borek"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"7352",title:"Granularity in Materials Science",subtitle:null,isOpenForSubmission:!1,hash:"a451ff13b9bc3b08989979518577594a",slug:"granularity-in-materials-science",bookSignature:"George Kyzas and Athanasios C. Mitropoulos",coverURL:"https://cdn.intechopen.com/books/images_new/7352.jpg",editedByType:"Edited by",editors:[{id:"152296",title:"Prof.",name:"George",middleName:"Z.",surname:"Kyzas",slug:"george-kyzas",fullName:"George Kyzas"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6512",title:"Superfluids and Superconductors",subtitle:null,isOpenForSubmission:!1,hash:"24385ec1d5de9c6597896900c80ee279",slug:"superfluids-and-superconductors",bookSignature:"Roberto Zivieri",coverURL:"https://cdn.intechopen.com/books/images_new/6512.jpg",editedByType:"Edited by",editors:[{id:"181334",title:"Prof.",name:"Roberto",middleName:null,surname:"Zivieri",slug:"roberto-zivieri",fullName:"Roberto Zivieri"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"5319",title:"Wetting and Wettability",subtitle:null,isOpenForSubmission:!1,hash:"49767cc09f266bd5bdf55f4a5c57792b",slug:"wetting-and-wettability",bookSignature:"Mahmood Aliofkhazraei",coverURL:"https://cdn.intechopen.com/books/images_new/5319.jpg",editedByType:"Edited by",editors:[{id:"155413",title:"Dr.",name:"Mahmood",middleName:null,surname:"Aliofkhazraei",slug:"mahmood-aliofkhazraei",fullName:"Mahmood Aliofkhazraei"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2854",title:"Viscoelasticity",subtitle:"From Theory to Biological Applications",isOpenForSubmission:!1,hash:"63c4a0eddb48f02ebe48d80aa70972de",slug:"viscoelasticity-from-theory-to-biological-applications",bookSignature:"Juan de Vicente",coverURL:"https://cdn.intechopen.com/books/images_new/2854.jpg",editedByType:"Edited by",editors:[{id:"99801",title:"Dr.",name:"Juan",middleName:null,surname:"De Vicente",slug:"juan-de-vicente",fullName:"Juan De Vicente"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],booksByTopicTotal:5,seriesByTopicCollection:[],seriesByTopicTotal:0,mostCitedChapters:[{id:"48816",doi:"10.5772/60824",title:"Wettability and Other Surface Properties of Modified Polymers",slug:"wettability-and-other-surface-properties-of-modified-polymers",totalDownloads:3701,totalCrossrefCites:9,totalDimensionsCites:36,abstract:"Surface wettability is one of the crucial characteristics for determining of a material’s use in specific application. Determination of wettability is based on the measurement of the material surface contact angle. Contact angle is the main parameter that characterizes the drop shape on the solid surface and is also one of the directly measurable properties of the phase interface. In this chapter, the wettability and its related properties of pristine and modified polymer foils will be described. The wettability depends on surface roughness and chemical composition. Changes of these parameters can adjust the values of contact angle and, therefore, wettability. In the case of pristine polymer materials, their wettability is unsuitable for a wide range of applications (such as tissue engineering, printing, and coating). Polymer surfaces can easily be modified by, e.g., plasma discharge, whereas the bulk properties remain unchanged. This modification leads to oxidation of the treated layer and creation of new chemical groups that mainly contain oxygen. Immediately after plasma treatment, the values of the contact angles of the modified polymer significantly decrease. In the case of a specific polymer, the strongly hydrophilic surface is created and leads to total spreading of the water drop. Wettability is strongly dependent on time from modification.",book:{id:"5319",slug:"wetting-and-wettability",title:"Wetting and Wettability",fullTitle:"Wetting and Wettability"},signatures:"Nikola Slepickova Kasalkova, Petr Slepicka, Zdenka Kolska and\nVaclav Svorcik",authors:[{id:"144929",title:"Prof.",name:"Vaclav",middleName:null,surname:"Svorcik",slug:"vaclav-svorcik",fullName:"Vaclav Svorcik"},{id:"146297",title:"Dr.",name:"Petr",middleName:null,surname:"Slepicka",slug:"petr-slepicka",fullName:"Petr Slepicka"},{id:"147600",title:"Ph.D.",name:"Nikola",middleName:null,surname:"Slepičková Kasálková",slug:"nikola-slepickova-kasalkova",fullName:"Nikola Slepičková Kasálková"},{id:"153983",title:"Dr.",name:"Zdeňka",middleName:null,surname:"Kolská",slug:"zdenka-kolska",fullName:"Zdeňka Kolská"}]},{id:"48822",doi:"10.5772/60808",title:"Wettability of Nanostructured Surfaces",slug:"wettability-of-nanostructured-surfaces",totalDownloads:3128,totalCrossrefCites:11,totalDimensionsCites:32,abstract:"There are many studies in literature concerning contact angle measurements on different materials/substrates. It is documented that textiles can be coated with multifunctional materials in form of thin films or nanoparticles to acquire characteristics that can improve the protection and comfort of the wearer. The capacity of oxide nanostructures to inhibit fungal development and neutralize bacteria is a direct consequence of their wetting behavior [1–6]. Moreover, the radical modification of wetting behavior of nanostructures from hydrophilic to hydrophobic when changing the pulsed laser deposition (PLD) ambient will be thoroughly discussed.",book:{id:"5319",slug:"wetting-and-wettability",title:"Wetting and Wettability",fullTitle:"Wetting and Wettability"},signatures:"L. Duta, A.C. Popescu, I. Zgura, N. Preda and I.N. Mihailescu",authors:[{id:"17636",title:"Prof.",name:"Ion N.",middleName:null,surname:"Mihailescu",slug:"ion-n.-mihailescu",fullName:"Ion N. Mihailescu"},{id:"23532",title:"Dr.",name:"Andrei",middleName:null,surname:"Popescu",slug:"andrei-popescu",fullName:"Andrei Popescu"},{id:"174343",title:"Dr.",name:"Liviu",middleName:null,surname:"Duta",slug:"liviu-duta",fullName:"Liviu Duta"},{id:"174344",title:"Dr.",name:"Irina",middleName:null,surname:"Zgura",slug:"irina-zgura",fullName:"Irina Zgura"},{id:"174345",title:"Dr.",name:"Ligia",middleName:null,surname:"Frunza",slug:"ligia-frunza",fullName:"Ligia Frunza"}]},{id:"49090",doi:"10.5772/61205",title:"The Wetting of Leaf Surfaces and Its Ecological Significances",slug:"the-wetting-of-leaf-surfaces-and-its-ecological-significances",totalDownloads:3521,totalCrossrefCites:13,totalDimensionsCites:25,abstract:"Leaf wettability, indicating the affinity for water on leaf surfaces, is a common phenomenon for plants in a wide variety of habitats. The contact angle (θ) of water on leaves measured at the gas, solid and liquid interface is an index of surface wettability. Leaves are termed as “super-hydrophilic” if θ < 40°, “highly wettable” if θ < 90°, and “wettable” if θ < 110°. If θ > 110°, the leaves are classified as being non-wettable, while θ > 130° for highly non-wettable and θ > 150° for super-hydrophobic. Both internal and external factors can influence leaf wettability. The chemical composition and structure of leaf surfaces are internal causes, but the external environment can also influence wettability by affecting the structure and composition of the surface. The main internal factors that affecting leaf wettability include the content and microstructure of the epidermal wax, the number, size and pattern of trichomes, stomatal density, the shape of epidermal cells, and leaf water status. The leaf contact angles increased with the increasing of leaf wax content. However, studies have shown that the contact angles were more dependent on the complexity of wax structure than on the absolute amount. For trichomes, there are three types of interaction between trichomes and water droplets, including (1) low trichomes density: no apparent influence of trichomes on the location of surface moisture, droplet formation and retention ; (2) medium trichomes density: trichomes appear to circle surface moisture into patches; (3) high trichomes density: trichomes appear to hold water droplets above the trichomes. In some cases, a higher stomatal density was accompanied with a higher contact angles. While, it was also observed that there was no significant correlation between contact angle and stomatal density for some species. For the effects of epidermal cells on leaf wettability, it was generally considered that the combination of a dense layer of surface wax and the convex epidermal cells was what created a hydrophobic leaf surface. However, the influence of leaf water content on contact angle of water droplets on different leaf surfaces was complex, e.g., contact angles increased with decreasing of leaf water content, contact angle remained to be constant with different leaf water content.",book:{id:"5319",slug:"wetting-and-wettability",title:"Wetting and Wettability",fullTitle:"Wetting and Wettability"},signatures:"Huixia Wang, Hui Shi and Yanhui Wang",authors:[{id:"173921",title:"Dr.",name:"Huixia",middleName:null,surname:"Wang",slug:"huixia-wang",fullName:"Huixia Wang"}]},{id:"40738",doi:"10.5772/49979",title:"Viscoelastic Properties of Biological Materials",slug:"viscoelastic-properties-of-biological-materials",totalDownloads:5572,totalCrossrefCites:12,totalDimensionsCites:24,abstract:null,book:{id:"2854",slug:"viscoelasticity-from-theory-to-biological-applications",title:"Viscoelasticity",fullTitle:"Viscoelasticity - From Theory to Biological Applications"},signatures:"Naoki Sasaki",authors:[{id:"140935",title:"Prof.",name:"Naoki",middleName:null,surname:"Sasaki",slug:"naoki-sasaki",fullName:"Naoki Sasaki"}]},{id:"40741",doi:"10.5772/50137",title:"Die Swell of Complex Polymeric Systems",slug:"die-swell-of-complex-polymeric-systems",totalDownloads:6070,totalCrossrefCites:3,totalDimensionsCites:17,abstract:null,book:{id:"2854",slug:"viscoelasticity-from-theory-to-biological-applications",title:"Viscoelasticity",fullTitle:"Viscoelasticity - From Theory to Biological Applications"},signatures:"Kejian Wang",authors:[{id:"141238",title:"Prof.",name:"Kejian",middleName:null,surname:"Wang",slug:"kejian-wang",fullName:"Kejian Wang"}]}],mostDownloadedChaptersLast30Days:[{id:"48768",title:"TiO2 -Based Surfaces with Special Wettability – From Nature to Biomimetic Application",slug:"tio2-based-surfaces-with-special-wettability-from-nature-to-biomimetic-application",totalDownloads:5046,totalCrossrefCites:3,totalDimensionsCites:4,abstract:"Super-wetting/antiwetting surfaces with extremely high contrast of surface energy and liquid adhesion have attracted a lot of interest in both fundamental research and industry. Various types of special wetting surfaces can be constructed by adjusting the topographical structure and chemical composition. In this chapter, recent advance of the super-wetting/antiwetting surfaces with special solid/liquid adhesion has been reviewed, with a focus on the biomimetic fabrication and applications of TiO2-based surfaces. Special super-wettability examples include lotus-leaf-inspired surfaces with low adhesion, rose-petal-inspired surfaces with high adhesion, spider silk bio-inspired surfaces with directional adhesion, fish-scale-inspired underwater superoleophobic surface, and artificial surfaces with controllable or stimuli-responsive liquid adhesion. In addition, we will review some potential applications related to artificial antiwetting surface with controllable adhesion, e.g., self-cleaning, antifogging/anti-icing, micro-droplet manipulation, fog/water collection, water/oil separation, anti-bioadhesion, micro-template for patterning, and friction reduction. Finally, the difficulty and prospects of this renascent and rapidly developing field are also briefly proposed and discussed.",book:{id:"5319",slug:"wetting-and-wettability",title:"Wetting and Wettability",fullTitle:"Wetting and Wettability"},signatures:"Jian-Ying Huang and Yue-Kun Lai",authors:[{id:"175512",title:"Prof.",name:"Yuekun",middleName:null,surname:"Lai",slug:"yuekun-lai",fullName:"Yuekun Lai"}]},{id:"62882",title:"Inside the Phenomenological Aspects of Wet Granulation: Role of Process Parameters",slug:"inside-the-phenomenological-aspects-of-wet-granulation-role-of-process-parameters",totalDownloads:1427,totalCrossrefCites:1,totalDimensionsCites:1,abstract:"Granulation is a size-enlargement process by which small particles are bonded, by means of various techniques, in coherent and stable masses (granules), in which the original particles are still identifiable. In wet granulation processes, the powder particles are aggregated through the use of a liquid phase called binder. The main purposes of size-enlargement process of a powder or mixture of powders are to improve technological properties and/or to realize suitable forms of commercial products. A modern and rational approach in the production of granular structures with tailored features (in terms of size and size distribution, flowability, mechanical and release properties, etc.) requires a deep understanding of phenomena involved during granules formation. By this knowledge, suitable predictive tools can be developed with the aim to choose right process conditions to be used in developing new formulations by avoiding or reducing costs for new tests. In this chapter, after introductive notes on granulation process, the phenomenological aspects involved in the formation of the granules with respect to the main process parameters are presented by experimental demonstration. Possible mathematical approaches in the granulation process description are also presented and the one involving the population mass balances equations is detailed.",book:{id:"7352",slug:"granularity-in-materials-science",title:"Granularity in Materials Science",fullTitle:"Granularity in Materials Science"},signatures:"Veronica De Simone, Diego Caccavo, Annalisa Dalmoro, Gaetano\nLamberti, Matteo d’Amore and Anna Angela Barba",authors:[{id:"140173",title:"Prof.",name:"Anna Angela",middleName:null,surname:"Barba",slug:"anna-angela-barba",fullName:"Anna Angela Barba"},{id:"143947",title:"Prof.",name:"Matteo",middleName:null,surname:"D'Amore",slug:"matteo-d'amore",fullName:"Matteo D'Amore"},{id:"176104",title:"Prof.",name:"Gaetano",middleName:null,surname:"Lamberti",slug:"gaetano-lamberti",fullName:"Gaetano Lamberti"},{id:"176239",title:"MSc.",name:"Diego",middleName:null,surname:"Caccavo",slug:"diego-caccavo",fullName:"Diego Caccavo"},{id:"181500",title:"Dr.",name:"Annalisa",middleName:null,surname:"Dalmoro",slug:"annalisa-dalmoro",fullName:"Annalisa Dalmoro"},{id:"260822",title:"MSc.",name:"Veronica",middleName:null,surname:"De Simone",slug:"veronica-de-simone",fullName:"Veronica De Simone"}]},{id:"49177",title:"Influence of Wettability and Reactivity on Refractory Degradation – Interactions of Molten Iron and Slags with Steelmaking Refractories at 1550°C",slug:"influence-of-wettability-and-reactivity-on-refractory-degradation-interactions-of-molten-iron-and-sl",totalDownloads:2068,totalCrossrefCites:0,totalDimensionsCites:1,abstract:"Refractories, materials that can withstand high temperatures, play an important role in the iron and steel sector which alone accounts for ~70% of total refractories produced. In this chapter, detailed wettability and interfacial phenomena investigations on alumina-carbon and zirconia-carbon refractories at steelmaking temperatures. The wettability between refractory substrates and molten iron/slags was investigated at 1550°C using the sessile drop approach in a horizontal tube furnace equipped with a CCD camera. Detailed experimental results were obtained on alumina-carbon/molten iron system at high temperatures. Alumina is known to be non-wetting to molten iron while carbon can be easily wetted. Observed contact angles were found to depend strongly on the substrate composition and contact time. While the refractory substrates containing 50 and 60% carbon were found to be non-wetting to molten iron, the substrates containing higher amounts of C (≥ 70%) were found to become increasingly wetting. Molten iron droplets were seen to spread on these substrates.",book:{id:"5319",slug:"wetting-and-wettability",title:"Wetting and Wettability",fullTitle:"Wetting and Wettability"},signatures:"R. Khanna, M. Ikram-ul-Haq and V. Sahajwalla",authors:[{id:"19010",title:"Associate Prof.",name:"Rita",middleName:null,surname:"Khanna",slug:"rita-khanna",fullName:"Rita Khanna"}]},{id:"48822",title:"Wettability of Nanostructured Surfaces",slug:"wettability-of-nanostructured-surfaces",totalDownloads:3131,totalCrossrefCites:11,totalDimensionsCites:32,abstract:"There are many studies in literature concerning contact angle measurements on different materials/substrates. It is documented that textiles can be coated with multifunctional materials in form of thin films or nanoparticles to acquire characteristics that can improve the protection and comfort of the wearer. The capacity of oxide nanostructures to inhibit fungal development and neutralize bacteria is a direct consequence of their wetting behavior [1–6]. Moreover, the radical modification of wetting behavior of nanostructures from hydrophilic to hydrophobic when changing the pulsed laser deposition (PLD) ambient will be thoroughly discussed.",book:{id:"5319",slug:"wetting-and-wettability",title:"Wetting and Wettability",fullTitle:"Wetting and Wettability"},signatures:"L. Duta, A.C. Popescu, I. Zgura, N. Preda and I.N. Mihailescu",authors:[{id:"17636",title:"Prof.",name:"Ion N.",middleName:null,surname:"Mihailescu",slug:"ion-n.-mihailescu",fullName:"Ion N. Mihailescu"},{id:"23532",title:"Dr.",name:"Andrei",middleName:null,surname:"Popescu",slug:"andrei-popescu",fullName:"Andrei Popescu"},{id:"174343",title:"Dr.",name:"Liviu",middleName:null,surname:"Duta",slug:"liviu-duta",fullName:"Liviu Duta"},{id:"174344",title:"Dr.",name:"Irina",middleName:null,surname:"Zgura",slug:"irina-zgura",fullName:"Irina Zgura"},{id:"174345",title:"Dr.",name:"Ligia",middleName:null,surname:"Frunza",slug:"ligia-frunza",fullName:"Ligia Frunza"}]},{id:"62615",title:"Nanolevel Surface Processing of Fine Particles by Waterjet Cavitation and Multifunction Cavitation to Improve the Photocatalytic Properties of Titanium Oxide",slug:"nanolevel-surface-processing-of-fine-particles-by-waterjet-cavitation-and-multifunction-cavitation-t",totalDownloads:1164,totalCrossrefCites:3,totalDimensionsCites:6,abstract:"Titanium oxide particles were treated by water jet cavitation (WJC) generated and multifunction cavitation (MFC) using an ejector nozzle. Generation, growth, and collapse of cavitation are repeated with the particles of titanium oxide and platinum. Because the cavitation has an extremely high collapse pressure, the surface of the titanium oxide particles is processed by the microjets of cavitation in a reactor comprising the ejector nozzle. In the multifunction cavitation, ultrasonic irradiation of a waterjet during floating cavitation was used to generate microjets with hot spots. Hot working can be performed at the nanoscale on a material surface using this MFC process, resulting in morphological changes and variations in the surface electrochemical characteristics. The fundamental characteristics of multifunction cavitation were investigated theoretically and experimentally. Furthermore, the additional nozzle was put on the ejector nozzle in order to increase the temperature and pressure of bubble and the mechanism was clarified. The quantities of hydrogen and oxygen generated from titanium dioxide particles treated by multifunction cavitation in response to UV and visible light irradiation were remarkably increased compared to the amounts produced by particles treated by WJC processing. In this chapter, the methods and their results of processing particles by cavitation are introduced.",book:{id:"7385",slug:"cavitation-selected-issues",title:"Cavitation",fullTitle:"Cavitation - Selected Issues"},signatures:"Toshihiko Yoshimura, Kumiko Tanaka and Masataka Ijiri",authors:[{id:"246052",title:"Dr.",name:"Masataka",middleName:null,surname:"Ijiri",slug:"masataka-ijiri",fullName:"Masataka Ijiri"},{id:"246359",title:"Prof.",name:"Toshihiko",middleName:null,surname:"Yoshimura",slug:"toshihiko-yoshimura",fullName:"Toshihiko Yoshimura"},{id:"246433",title:"Dr.",name:"Kumiko",middleName:null,surname:"Tanaka",slug:"kumiko-tanaka",fullName:"Kumiko Tanaka"}]}],onlineFirstChaptersFilter:{topicId:"961",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:0,limit:8,total:null},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:87,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:99,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:27,numberOfPublishedChapters:288,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:139,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:0,numberOfUpcomingTopics:2,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!1},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:107,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:0,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!1},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:11,numberOfOpenTopics:4,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}}]},series:{item:{id:"25",title:"Environmental Sciences",doi:"10.5772/intechopen.100362",issn:"2754-6713",scope:"
\r\n\tScientists have long researched to understand the environment and man’s place in it. The search for this knowledge grows in importance as rapid increases in population and economic development intensify humans’ stresses on ecosystems. Fortunately, rapid increases in multiple scientific areas are advancing our understanding of environmental sciences. Breakthroughs in computing, molecular biology, ecology, and sustainability science are enhancing our ability to utilize environmental sciences to address real-world problems. \r\n\tThe four topics of this book series - Pollution; Environmental Resilience and Management; Ecosystems and Biodiversity; and Water Science - will address important areas of advancement in the environmental sciences. They will represent an excellent initial grouping of published works on these critical topics.
",coverUrl:"https://cdn.intechopen.com/series/covers/25.jpg",latestPublicationDate:"April 13th, 2022",hasOnlineFirst:!1,numberOfPublishedBooks:1,editor:{id:"197485",title:"Dr.",name:"J. Kevin",middleName:null,surname:"Summers",slug:"j.-kevin-summers",fullName:"J. Kevin Summers",profilePictureURL:"https://mts.intechopen.com/storage/users/197485/images/system/197485.jpg",biography:"J. Kevin Summers is a Senior Research Ecologist at the Environmental Protection Agency’s (EPA) Gulf Ecosystem Measurement and Modeling Division. He is currently working with colleagues in the Sustainable and Healthy Communities Program to develop an index of community resilience to natural hazards, an index of human well-being that can be linked to changes in the ecosystem, social and economic services, and a community sustainability tool for communities with populations under 40,000. He leads research efforts for indicator and indices development. Dr. Summers is a systems ecologist and began his career at the EPA in 1989 and has worked in various programs and capacities. This includes leading the National Coastal Assessment in collaboration with the Office of Water which culminated in the award-winning National Coastal Condition Report series (four volumes between 2001 and 2012), and which integrates water quality, sediment quality, habitat, and biological data to assess the ecosystem condition of the United States estuaries. He was acting National Program Director for Ecology for the EPA between 2004 and 2006. He has authored approximately 150 peer-reviewed journal articles, book chapters, and reports and has received many awards for technical accomplishments from the EPA and from outside of the agency. Dr. Summers holds a BA in Zoology and Psychology, an MA in Ecology, and Ph.D. in Systems Ecology/Biology.",institutionString:null,institution:{name:"Environmental Protection Agency",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:4,paginationItems:[{id:"3",title:"Bacterial Infectious Diseases",coverUrl:"https://cdn.intechopen.com/series_topics/covers/3.jpg",isOpenForSubmission:!1,editor:null,editorTwo:null,editorThree:null},{id:"4",title:"Fungal Infectious Diseases",coverUrl:"https://cdn.intechopen.com/series_topics/covers/4.jpg",isOpenForSubmission:!0,editor:{id:"174134",title:"Dr.",name:"Yuping",middleName:null,surname:"Ran",slug:"yuping-ran",fullName:"Yuping Ran",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bS9d6QAC/Profile_Picture_1630330675373",biography:"Dr. Yuping Ran, Professor, Department of Dermatology, West China Hospital, Sichuan University, Chengdu, China. Completed the Course Medical Mycology, the Centraalbureau voor Schimmelcultures (CBS), Fungal Biodiversity Centre, Netherlands (2006). International Union of Microbiological Societies (IUMS) Fellow, and International Emerging Infectious Diseases (IEID) Fellow, Centers for Diseases Control and Prevention (CDC), Atlanta, USA. Diploma of Dermatological Scientist, Japanese Society for Investigative Dermatology. Ph.D. of Juntendo University, Japan. Bachelor’s and Master’s degree, Medicine, West China University of Medical Sciences. Chair of Sichuan Medical Association Dermatology Committee. General Secretary of The 19th Annual Meeting of Chinese Society of Dermatology and the Asia Pacific Society for Medical Mycology (2013). In charge of the Annual Medical Mycology Course over 20-years authorized by National Continue Medical Education Committee of China. Member of the board of directors of the Asia-Pacific Society for Medical Mycology (APSMM). Associate editor of Mycopathologia. Vice-chief of the editorial board of Chinses Journal of Mycology, China. Board Member and Chair of Mycology Group of Chinese Society of Dermatology.",institutionString:null,institution:{name:"Sichuan University",institutionURL:null,country:{name:"China"}}},editorTwo:null,editorThree:null},{id:"5",title:"Parasitic Infectious Diseases",coverUrl:"https://cdn.intechopen.com/series_topics/covers/5.jpg",isOpenForSubmission:!0,editor:{id:"67907",title:"Dr.",name:"Amidou",middleName:null,surname:"Samie",slug:"amidou-samie",fullName:"Amidou Samie",profilePictureURL:"https://mts.intechopen.com/storage/users/67907/images/system/67907.jpg",biography:"Dr. Amidou Samie is an Associate Professor of Microbiology at the University of Venda, in South Africa, where he graduated for his PhD in May 2008. He joined the Department of Microbiology the same year and has been giving lectures on topics covering parasitology, immunology, molecular biology and industrial microbiology. He is currently a rated researcher by the National Research Foundation of South Africa at category C2. He has published widely in the field of infectious diseases and has overseen several MSc’s and PhDs. His research activities mostly cover topics on infectious diseases from epidemiology to control. His particular interest lies in the study of intestinal protozoan parasites and opportunistic infections among HIV patients as well as the potential impact of childhood diarrhoea on growth and child development. He also conducts research on water-borne diseases and water quality and is involved in the evaluation of point-of-use water treatment technologies using silver and copper nanoparticles in collaboration with the University of Virginia, USA. He also studies the use of medicinal plants for the control of infectious diseases as well as antimicrobial drug resistance.",institutionString:null,institution:{name:"University of Venda",institutionURL:null,country:{name:"South Africa"}}},editorTwo:null,editorThree:null},{id:"6",title:"Viral Infectious Diseases",coverUrl:"https://cdn.intechopen.com/series_topics/covers/6.jpg",isOpenForSubmission:!0,editor:{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}},editorTwo:null,editorThree:null}]},overviewPageOFChapters:{paginationCount:4,paginationItems:[{id:"81821",title:"Pneumococcal Carriage in Jordanian Children and the Importance of Vaccination",doi:"10.5772/intechopen.104999",signatures:"Adnan Al-Lahham",slug:"pneumococcal-carriage-in-jordanian-children-and-the-importance-of-vaccination",totalDownloads:1,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Streptococcal Infections",coverURL:"https://cdn.intechopen.com/books/images_new/10828.jpg",subseries:{id:"3",title:"Bacterial Infectious Diseases"}}},{id:"81813",title:"Schistosomiasis: Discovery of New Molecules for Disease Treatment and Vaccine Development",doi:"10.5772/intechopen.104738",signatures:"Andressa Barban do Patrocinio",slug:"schistosomiasis-discovery-of-new-molecules-for-disease-treatment-and-vaccine-development",totalDownloads:5,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"New Horizons for Schistosomiasis Research",coverURL:"https://cdn.intechopen.com/books/images_new/10829.jpg",subseries:{id:"5",title:"Parasitic Infectious Diseases"}}},{id:"81644",title:"Perspective Chapter: Ethics of Using Placebo Controlled Trials for Covid-19 Vaccine Development in Vulnerable Populations",doi:"10.5772/intechopen.104776",signatures:"Lesley Burgess, Jurie Jordaan and Matthew Wilson",slug:"perspective-chapter-ethics-of-using-placebo-controlled-trials-for-covid-19-vaccine-development-in-vu",totalDownloads:9,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"SARS-CoV-2 Variants - Two Years After",coverURL:"https://cdn.intechopen.com/books/images_new/11573.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}},{id:"80546",title:"Streptococcal Skin and Skin-Structure Infections",doi:"10.5772/intechopen.102894",signatures:"Alwyn Rapose",slug:"streptococcal-skin-and-skin-structure-infections",totalDownloads:48,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Streptococcal Infections",coverURL:"https://cdn.intechopen.com/books/images_new/10828.jpg",subseries:{id:"3",title:"Bacterial Infectious Diseases"}}}]},overviewPagePublishedBooks:{paginationCount:13,paginationItems:[{type:"book",id:"6667",title:"Influenza",subtitle:"Therapeutics and Challenges",coverURL:"https://cdn.intechopen.com/books/images_new/6667.jpg",slug:"influenza-therapeutics-and-challenges",publishedDate:"September 19th 2018",editedByType:"Edited by",bookSignature:"Shailendra K. Saxena",hash:"105e347b2d5dbbe6b593aceffa051efa",volumeInSeries:1,fullTitle:"Influenza - Therapeutics and Challenges",editors:[{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}}]},{type:"book",id:"7064",title:"Current Perspectives in Human Papillomavirus",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7064.jpg",slug:"current-perspectives-in-human-papillomavirus",publishedDate:"May 2nd 2019",editedByType:"Edited by",bookSignature:"Shailendra K. Saxena",hash:"d92a4085627bab25ddc7942fbf44cf05",volumeInSeries:2,fullTitle:"Current Perspectives in Human Papillomavirus",editors:[{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}}]},{type:"book",id:"7123",title:"Current Topics in Neglected Tropical Diseases",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7123.jpg",slug:"current-topics-in-neglected-tropical-diseases",publishedDate:"December 4th 2019",editedByType:"Edited by",bookSignature:"Alfonso J. Rodriguez-Morales",hash:"61c627da05b2ace83056d11357bdf361",volumeInSeries:3,fullTitle:"Current Topics in Neglected Tropical Diseases",editors:[{id:"131400",title:"Prof.",name:"Alfonso J.",middleName:null,surname:"Rodriguez-Morales",slug:"alfonso-j.-rodriguez-morales",fullName:"Alfonso J. Rodriguez-Morales",profilePictureURL:"https://mts.intechopen.com/storage/users/131400/images/system/131400.png",biography:"Dr. Rodriguez-Morales is an expert in tropical and emerging diseases, particularly zoonotic and vector-borne diseases (especially arboviral diseases). He is the president of the Travel Medicine Committee of the Pan-American Infectious Diseases Association (API), as well as the president of the Colombian Association of Infectious Diseases (ACIN). He is a member of the Committee on Tropical Medicine, Zoonoses, and Travel Medicine of ACIN. He is a vice-president of the Latin American Society for Travel Medicine (SLAMVI) and a Member of the Council of the International Society for Infectious Diseases (ISID). Since 2014, he has been recognized as a Senior Researcher, at the Ministry of Science of Colombia. He is a professor at the Faculty of Medicine of the Fundacion Universitaria Autonoma de las Americas, in Pereira, Risaralda, Colombia. He is an External Professor, Master in Research on Tropical Medicine and International Health, Universitat de Barcelona, Spain. He is also a professor at the Master in Clinical Epidemiology and Biostatistics, Universidad Científica del Sur, Lima, Peru. In 2021 he has been awarded the “Raul Isturiz Award” Medal of the API. Also, in 2021, he was awarded with the “Jose Felix Patiño” Asclepius Staff Medal of the Colombian Medical College, due to his scientific contributions to COVID-19 during the pandemic. 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In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. 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She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. She is an author of about 90 publications (According to Scopus: H-Index: 23; According to WOS: H-Index: 20) on peer-reviewed journals, a member of the “Società Italiana di Biochimica e Biologia Molecolare,“ and a Consultant Reviewer for International Journal of Molecular Science, Journal of Chromatography A, COPD, Plos ONE and Nutritional Neuroscience.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null,series:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983"},editorialBoard:[{id:"72288",title:"Dr.",name:"Arli Aditya",middleName:null,surname:"Parikesit",slug:"arli-aditya-parikesit",fullName:"Arli Aditya Parikesit",profilePictureURL:"https://mts.intechopen.com/storage/users/72288/images/system/72288.jpg",institutionString:null,institution:{name:"Indonesia International Institute for Life Sciences",institutionURL:null,country:{name:"Indonesia"}}},{id:"40928",title:"Dr.",name:"Cesar",middleName:null,surname:"Lopez-Camarillo",slug:"cesar-lopez-camarillo",fullName:"Cesar Lopez-Camarillo",profilePictureURL:"https://mts.intechopen.com/storage/users/40928/images/3884_n.png",institutionString:null,institution:{name:"Universidad Autónoma de la Ciudad de México",institutionURL:null,country:{name:"Mexico"}}},{id:"81926",title:"Dr.",name:"Shymaa",middleName:null,surname:"Enany",slug:"shymaa-enany",fullName:"Shymaa Enany",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRqB9QAK/Profile_Picture_1626163237970",institutionString:null,institution:{name:"Suez Canal University",institutionURL:null,country:{name:"Egypt"}}}]},onlineFirstChapters:{paginationCount:1,paginationItems:[{id:"81831",title:"Deep Network Model and Regression Analysis using OLS Method for Predicting Lung Vital Capacity",doi:"10.5772/intechopen.104737",signatures:"Harun Sümbül",slug:"deep-network-model-and-regression-analysis-using-ols-method-for-predicting-lung-vital-capacity",totalDownloads:3,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Decision Science - 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Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. 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Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},subseries:[{id:"14",title:"Cell and Molecular Biology",keywords:"Omics (Transcriptomics; Proteomics; Metabolomics), Molecular Biology, Cell Biology, Signal Transduction and Regulation, Cell Growth and Differentiation, Apoptosis, Necroptosis, Ferroptosis, Autophagy, Cell Cycle, Macromolecules and Complexes, Gene Expression",scope:"The Cell and Molecular Biology topic within the IntechOpen Biochemistry Series aims to rapidly publish contributions on all aspects of cell and molecular biology, including aspects related to biochemical and genetic research (not only in humans but all living beings). We encourage the submission of manuscripts that provide novel and mechanistic insights that report significant advances in the fields. Topics include, but are not limited to: Advanced techniques of cellular and molecular biology (Molecular methodologies, imaging techniques, and bioinformatics); Biological activities at the molecular level; Biological processes of cell functions, cell division, senescence, maintenance, and cell death; Biomolecules interactions; Cancer; Cell biology; Chemical biology; Computational biology; Cytochemistry; Developmental biology; Disease mechanisms and therapeutics; DNA, and RNA metabolism; Gene functions, genetics, and genomics; Genetics; Immunology; Medical microbiology; Molecular biology; Molecular genetics; Molecular processes of cell and organelle dynamics; Neuroscience; Protein biosynthesis, degradation, and functions; Regulation of molecular interactions in a cell; Signalling networks and system biology; Structural biology; Virology and microbiology.",annualVolume:11410,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"79367",title:"Dr.",name:"Ana Isabel",middleName:null,surname:"Flores",fullName:"Ana Isabel Flores",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRpIOQA0/Profile_Picture_1632418099564",institutionString:null,institution:{name:"Hospital Universitario 12 De Octubre",institutionURL:null,country:{name:"Spain"}}},{id:"328234",title:"Ph.D.",name:"Christian",middleName:null,surname:"Palavecino",fullName:"Christian Palavecino",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000030DhEhQAK/Profile_Picture_1628835318625",institutionString:null,institution:{name:"Central University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"186585",title:"Dr.",name:"Francisco Javier",middleName:null,surname:"Martin-Romero",fullName:"Francisco Javier Martin-Romero",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB3HQAW/Profile_Picture_1631258137641",institutionString:null,institution:{name:"University of Extremadura",institutionURL:null,country:{name:"Spain"}}}]},{id:"15",title:"Chemical Biology",keywords:"Phenolic Compounds, Essential Oils, Modification of Biomolecules, Glycobiology, Combinatorial Chemistry, Therapeutic peptides, Enzyme Inhibitors",scope:"Chemical biology spans the fields of chemistry and biology involving the application of biological and chemical molecules and techniques. 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Behind these definitions are hidden all the aspects of normal and pathological functioning of all processes that the topic ‘Metabolism’ will cover within the Biochemistry Series. 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Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. Currently, proteomics relies mainly on mass spectrometry (MS) combined with electrophoretic (1 or 2-DE-MS) and/or chromatographic techniques (LC-MS/MS). MS is an excellent tool that has gained popularity in proteomics because of its ability to gather a complex body of information such as cataloging protein expression, identifying protein modification sites, and defining protein interactions. 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