Open access peer-reviewed chapter

Soybean and Other Legume Proteins Exhibit Beneficial Physiological Effects on Metabolic Syndrome and Inflammatory-Related Disorders

Written By

Mitsutaka Kohno

Submitted: 10 May 2021 Reviewed: 17 August 2021 Published: 21 September 2021

DOI: 10.5772/intechopen.99955

From the Edited Volume

Legumes Research - Volume 2

Edited by Jose C. Jimenez-Lopez and Alfonso Clemente

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Abstract

There is currently a trend in Western countries to increase the intake of plant proteins. In this chapter, the author explains that this is due to the beneficial physiological functions of plant proteins, based on the latest literature review and our own research results. Among plant proteins, soy protein has been reported to have many beneficial effects on the improvement and prevention of metabolic syndrome. This chapter outlines the excellent effects of soy protein on renal function [improvement of early symptoms of diabetic nephropathy], which is closely related to metabolic syndrome, and the effects of combining these effects as complementary medicine. In addition, recent findings about the anti-inflammatory and immune activation effects of soy protein as hydrolyzed peptides are outlined. A brief introduction of the recent results of other legume-derived proteins that have replaced soy proteins are also explained. By further deepening our understanding of the superior physiological functions of plant proteins, it is hoped that their use expands even further.

Keywords

  • soy protein
  • metabolic syndrome
  • chronic kidney disease
  • inflammatory disorder
  • pea protein
  • lupin protein
  • mung bean protein

1. Introduction

Protein is not only significant as an energy source, but also as a component of the body, such as muscle and connective tissue, and as a physiological function substance, such as enzymes, hormones, and immune antibodies.

On the other hand, the problem of food shortage (in particular, protein) due to global population growth is becoming increasingly serious. Because of the economic development of emerging countries, people who used to consume energy from “carbohydrates” such as bread and rice are now tending to consume “proteins” such as meat and seafood as a luxury item, and there are concerns about a shortage of protein supply on a global scale. Under these circumstances, the effective use of plant proteins as a protein source has been attracting attention. Plant proteins have been considered to be less adaptable to human tastes in terms of flavor and physical properties than animal proteins, but recent superior food processing technologies have led to the marketing of “delicious” plant protein foods that are at the same level as animal protein foods.

It has been reported that plant proteins, especially soy proteins, have beneficial functions to improve and prevent lifestyle-related diseases that cannot be overcome by animal proteins, which are currently prevalent all over the world. The US Food and Drug Administration [FDA] has approved the health claim for food labelling that the consumption of 25 g of soy protein per day reduces the risk of heart disease [1]. In Japan, the Consumer Affairs Agency [formerly the Ministry of Health, Labour and Welfare] has allowed the health labelling of soy protein as a food for specified health use “to people who are concerned about cholesterol levels” [FOSHU].

The beneficial physiological effects of soy protein are presumed to be due to anti-inflammatory properties. The anti-inflammatory effect of soy protein is enhanced by its processing into peptides. Indeed, it has been reported that soy peptides suppress muscle inflammation pain relief in rheumatoid arthritis and ameliorate inflammatory bowel disease.

Recently, it is being reported that not only soy proteins, but also some legume-derived proteins have excellent physiological effects that are similar to, or even absent from, soy proteins. In this chapter, the author 1] introduces the beneficial physiological effects of soy protein for MetS, CKD and inflammation; 2] reports that these effects acted complementarily when used in combination with drugs; and 3] suggests other legume-derived proteins as alternatives to soy protein as novel proteins from legumes with beneficial physiological functions.

By understanding these findings, it is hoped that plant proteins will be used more actively to contribute to the improvement of human health, as well as their value as protein nutrition, which is in short supply worldwide.

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2. Soy protein and peptides

2.1 Soy protein for metabolic syndrome (MetS)

The concept of MetS has been proposed by several committees. The first formalized concept of MetS was proposed by a consultation group for the definition of diabetes for the World Health Organization (WHO); it was determined to have a high-risk status with multiple risk factors for cardiovascular disease. This group emphasized insulin resistance as the major underlying factor [2]. In 2001, a definition for MetS was devised by the National Cholesterol Education Program (NCEP) Adult Treatment Panel III (ATP III) [3]. The American Heart Association and the National Heart Lung and Blood Institute updated this definition in 2005 [4]. This updated definition is one of the most widely used criteria for MetS. The International Diabetes Foundation (IDF) published new criteria for MetS [5] in 2005. Although it includes the same general criteria as the other definitions, it requires that obesity, but not necessarily insulin resistance, be present. Although visceral obesity is now recognized as an important factor, the IDF definition has been criticized for its emphasis on obesity, rather than insulin resistance, in pathophysiology [6].

In Japan, in 2006, MetS was defined as a multiple risk factor clustering syndrome caused by visceral fat accumulation and insulin resistance that accompanies this accumulation [7]. In the MetS stage, it is advocated that lifestyle intervention to reduce visceral adiposity should be given priority over drug treatment. Subjects with multiple risk factor syndrome were diagnosed with MetS if their visceral fat areas determined by CT scan were over 100 cm2.

The Japanese Committee for the Definition and Diagnosis of MetS aimed to select subjects with multiple risk factors in which lifestyle modification to reduce visceral adiposity has priority over drug treatment [8]. For this purpose, the Japanese government started a new health policy that provides a specific health check-up followed by specific counseling for subjects diagnosed with MetS according to the Japanese criteria from 2008.

Soy protein exerts not only conventional nutritional value but also beneficial effects on human health. Many randomized controlled trials (RCTs) have assessed the effects of soy products on serum lipids. Systematic reviews and meta-analyses have reported improvements in lipid metabolism (Table 1) [9, 10, 11, 12, 13, 14, 15, 16].

TitleNumber of articlesNumber of total subjectsOutcome [significant difference]Reference
Meta-analysis of the effects of soy protein intake on serum lipids.38743Total-C↓, LDL-C↓, TG↓[9]
Meta-analysis of the effects of soy protein containing isoflavones on the lipid profiles.231,381Total-C↓, LDL-C↓, TG↓, HDL-C↑[10]
A meta-analysis of the effect of soy protein supplementation on serum lipids.411,756Total-C↓, LDL-C↓, TG↓, HDL-C↑[11]
Hypocholesterolaemic effects of soya proteins: result of recent studies are predictable from Anderson meta-analysis data.27923Total-C↓, LDL-C↓[12]
Soy protein effects on serum lipoproteins: a quality assessment and meta-analysis of randomized, controlled studies.431,946LDL-C↓, TG↓, HDL-C↑[13]
Effect of Plant Protein on Blood Lipids: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.112 (non-soy; 18)5,774 (non-soy; 599)LDL-C↓, Non-HDL-C↓, Apo-B↓[14]
A Meta-Analysis of 46 Studies Identified by the FDA Demonstrates that Soy Protein Decreases Circulating LDL and Total Cholesterol Concentrations in Adults.432,607Total-C↓, LDL-C↓[15]
The effects of isolated soy protein, isolated soy isoflavones and soy protein containing isoflavones on serum lipids in postmenopausal women: A systematic review and meta-analysis.46Total-C↓, LDL-C↓, TG↓, HDL-C↓, hypercholesterolemic subjects’ Apo-A1↓[16]

Table 1.

Meta-analysis on improving lipid metabolism in soy protein.

Note: ↓ and ↑ signs represent decrease and increase, respectively, after supplement of active compounds. Total-cholesterol (Total-C); low-density lipoprotein cholesterol (LDL-C); triglyceride (TG); high-density lipoprotein cholesterol (HDL-C); non-high-density lipoprotein cholesterol (non-HDL-C); apo-lipoprotein-B (Apo-B); apo-lipoprotein-AI (Apo-AI).

Soy protein isolate [SPI] is composed of three major components, glycinin [approx. 40%], β-conglycinin [approx. 20%], and lipophilic proteins (approx. 40%) [17]. Glycinin and β-conglycinin are storage proteins in soy, and lipophilic proteins consist primarily of membrane proteins. Among these components, β-conglycinin has the function of lowering serum triglycerides preferentially over serum cholesterol [18]. Digestive decomposition products of β-conglycinin were reported that lowering the activity of fatty acid synthase and increasing the activities of β-oxidation enzymes, and the fecal excretion of TG was high in β-conglycinin-fed mice and rats [19, 20]. Therefore, in the calculation based on the recommendation by the FDA, the same effect can be expected with 5 g of β-conglycinin. In clinical study, daily consumption of 5 g of β-conglycinin per subject significantly lowered serum TG concentrations, and the apo B and VLDL-TG concentrations were significantly decreased [21]. Hence, β-conglycinin consumption may specifically affect TG metabolism. In addition, the intake of 5 g of β-conglycinin per day decreased the body fat ratio and visceral fat [21, 22]. Additionally, serum adiponectin significantly increased with the consumption of β-conglycinin, and serum free fatty acids in the β-conglycinin group were significantly decreased. Tachibana et al. showed that β-conglycinin improves insulin sensitivity in rats [23]. β-conglycinin might be an important food component for the prevention and/or amelioration of visceral fat syndrome, which is also called MetS (Table 2) [21, 22, 24, 25, 26, 27].

Study titleDesign of studyNumber of subjectsDuration of studyDose of β-conglycininOutcome [significant difference]Reference
Decrease in serum triacylglycerol and visceral fat mediated by dietary soybean β-conglycinin*1.Randomized, Double-Blind, Placebo-Controlled StudyTest1;138
Test2:102
Test1:12-wk
Test2:20-wk*1
4.4 g/dayTest1; TG↓, Apo-B↓, VLDL-TG↓
Test2; Visceral fat↓
[21]
Effects of soybean beta-conglycinin on body fat ratio and serum lipid levels in healthy volunteers of female university students.Randomized, single-blinded crossover design418-wk4.4 g/dayBody fat ratio↓[22]
Serum triacylglycerol-lowering effect of soybean β-conglycinin in mildly hypertriacylglycerolemic individuals.Randomized, Double-Blind, Placebo-Controlled Study6812-wk2.3 g/dayTG↓, HDL-C↑, Apo C-II↓[24]
Serum lipid-improving effect of soyabean β-conglycinin in hyperlipidaemic menopausal women.Randomized, Double-Blind, Placebo-Controlled Study10012-wk2.3 g/4.6 gTG↓, LDL-C↓, Apo-B↓, NEFA↓[25]
Improvement of Triglyceride Levels through the Intake of Enriched-β-Conglycinin Soybean (Nanahomare).Randomized, Double-Blind, Placebo-Controlled Study13412-wk38.8 g/weekTG↓[26]
Effects of beta-conglycinin intake on circulating FGF21 levels and brown adipose tissue activity in Japanese young men.Single-blinded randomized crossover trial212-wk9.2 g/dayFGF21↓, BAT act↑[27]

Table 2.

Clinical studies for lipid metabolism improvements of β-conglycinin.

Note: *1; Test 1 is an examination of the serum triglyceride level and Test 2 is a measure of visceral fat by means of CT scanning. ↓ and ↑ signs represent decrease and increase, respectively, after supplement of β-conglycinin. Triglyceride (TG); apo-lipoprotein-B (Apo-B); very low-density lipoprotein triglyceride (VLDL-TG); high-density lipoprotein cholesterol (HDL-C); apo-lipoprotein-CII (Apo-CII); low-density lipoprotein cholesterol (LDL-C); non-esterified fatty acid (NEFA); fibroblast growth factor 21 (FGF21); brown adipo tissue activity (BAT act).

2.2 Soy protein for chronic kidney disease

Chronic kidney disease [CKD] is a major public health burden, with a global prevalence of ~11% in the general adult population [28]. If left untreated, CKD slowly progresses to end-stage renal disease, which requires dialysis or kidney transplant. Worldwide, a 31.7% increase in CKD mortality was observed over the last decade [29]. Effective interventions to prevent and delay the progression of CKD are well recognized. Prevention should start at the government level with the institution of multisectoral polices supporting sustainable development goals [SDGs] and ensuring safe and healthy environments.

CKD is bidirectionally associated with MetS and cardiovascular diseases [CVDs] [30, 31], and diabetic nephropathy [DN] is a complication of diabetes [32]. Moreover, it has been reported that 40% of patients undergoing dialysis are doing so because of DN [33], and approximately 50% of type II diabetes patients exhibit urinary albumin disease, which is an early stage of DN [34].

For CKD prevention, it is important to gain insight about commonly consumed foods and beverages in relation to kidney function. A report has been published in which PubMed was comprehensively searched for papers published until August 2019 describing prospective cohort studies and was supplemented by manual searches of reference lists from appropriate studies [35]. In this report, there was convincing evidence that a healthy dietary pattern may lower CKD risk. Red (processed) meat, poultry, fish, dairy, vegetables, legumes, nuts, and fruits were recommended foods for CKD patients. Dietary patterns were recommended adherence to the Dietary Approach to Stop Hypertension (DASH) diet, Mediterranean diet, and other healthy dietary patterns. As unhealthy diets, high-fat and high-sugar diets and high-acid-loaded diets were pointed out. In the Atherosclerosis Risk in Communities [ARIC] study of ~12,000 US participants with 23 years of follow-up, consumption of legumes was significantly associated with lower risks of CKD, with an HR of 0.83 [95% CI, 0.72; 0.95] for high versus low intakes [36]. Soy protein, which is representative of legumes, has been reported to suppress the progression of DN [37, 38]. The effects of soy protein on DN/CKD in clinical trials are summarized in Table 3 [39, 40, 41, 42, 43].

Study titleDesign of studyNumber of subjectsDuration of studyOutcome [significant difference]Reference
Soy protein intake, cardiorenal indices, and c-reactive protein in type 2 diabetes with nephropathy.Longitudinal randomized clinical trial study414-yFPG↓, Total-C↓, LDL-C↓, TG↓, CRP↓, Proteinuria↓, Urinary creatinine↓[39]
The effects of soy protein on chronic kidney disease: a meta-analysis of randomized controlled trials.Meta-analysis [9 studies]Total 1976-wk~4-ySerum creatinine↓, Phosphorus↓, TG↓[40]
Soy-based renoprotection.Single arm intervention (3 studies)
Placebo-controlled chronic intervention [22 studies]
Total 6344-wk~6-moTotal-C↓, LDL-C↓, Urinary creatinine↓, [Urinary albumin↓][41]
Effects of soy protein containing isoflavones in patients with chronic kidney disease: A systematic review and meta-analysis.Meta-analysis [12 studies]Total 280Serum creatine↓, Phosphorus↓, CRP↓, Proteinuria↓, BUN↓ [in predialysis subgroup][42]
Soy Protein and Chronic Kidney Disease: An Updated Review.RCT (3 studies), DBRCT, CRCT, LRCT
Total 6 studies
Total 3351~24-wkUrinary urea nitrogen↓, Proteinuria↓, Blood sodium↓, Serum Creatinine↓[43]

Table 3.

Summary of clinical studies by soy protein for CKD.

Note: ↓ sign represents decrease, after supplement of soy protein. Fasting plasma glucose [FPG]; total-cholesterol (Total-C); low-density lipoprotein cholesterol (LDL-C); C-reactive protein [CRP], blood urea nitrogen (BUN).

Kidney disease patients are carefully monitored for protein intake, and restricted protein intake according to the progression of their condition by doctors and nutritionists. However, there are some reports showing that mild protein restriction does not suppress the progression of kidney disease [44, 45, 46]. Therefore, it is necessary to consider not only the quantity but also the quality of protein. Legumes, including soy protein, can be regarded as very significant proteins to help treat nephropathy.

2.3 Anti-inflammatory roles of soy protein and peptides

Inflammation can occur when infectious microorganisms such as bacteria, viruses, and fungi invade the body and circulate in the blood, and/or when they enter certain tissues [47, 48]. Inflammation can also occur during the course of pathologies such as tissue damage, cell death, cancer, ischemia, and degeneration [49, 50, 51].

There are reports of the anti-inflammatory effects of soy protein and its hydrolysate peptides [52]. Among them, lunasin is considered one of the most studied bioactive peptides. Since its discovery in soybean twenty years ago, many researchers around the world have focused their studies on demonstrating the chemo preventive and chemotherapeutic activity of lunasin [53, 54, 55]. Lunasin is a 44 amino acid peptide isolated from soy that has three domains implicated in anticancer activity: an RGD motif [Arg-Gly-Asp], a helical domain with a sequence conserved in chromatin binding proteins [Glu-Lys-His-Ile-Met-Glu-Lys-Ile], and a poly-aspartic acid tail [56]. Lunacin has been reported to have unique antioxidant, anti-inflammatory, and anti-cancer properties, and to play an important role in the regulation of cholesterol biosynthesis in the body [57]. Lunacin has potential as a dietary supplement by its high bioavailability and thermal stability.

Trypsin digests of soy proteins revealed that the sequence MITLAIPVNKPGR was able to stimulate phagocytosis in leukocytes. This peptide derived from β-conglycinin was named “Soymetide”. The Met at its N-terminus was essential for its activity [58]. Four residues of the C-terminal residues of Soymetide-13 could be removed to form Soymetide-9 [MITLAIPVN], which had the highest activity. In these 9 residues [Soymetide-9], Soymetide-4 [MITL] is the minimal sequence required for its activity [58].

Soy protein with or without isoflavones was shown to reduce oxidative stress and have anti-inflammatory properties by inhibiting nuclear factor-kappa B [NF-κB] and blocking the secretion of pro-inflammatory cytokines in model rats and mouse. In clinical study by subjects with end-stage renal disease and healthy women over 70 years of age, their oxidative stress and inflammatory symptom were reduced [59]. The bioactive peptides RQRK and VIK were produced by digestion with pepsin and pancreatin from soy milk. These peptides inhibited lipopolysaccharide-induced inflammation in murine macrophages and the production of nitric oxide, interleukin [I])-1, nitric oxide synthase, and cyclooxygenase-2 [60].

Inflammatory bowel disease [IBD] is an intractable disease that causes inflammation of the gastrointestinal tract. Ulcerative colitis and Crohn’s disease are the two major pathologies of IBD [61]. Ulcerative colitis is a non-specific inflammatory disorder that causes ulcers and erosion, primarily in the colonic mucosa. Young et al. revealed that soy peptides were effective in preventing dextran sulphate sodium[(DSS)-induced colitis in pigs [62]. The soy-derived tripeptide Val-Pro-Tyr [VPY] has been reported that anti-inflammatory effects in Caco-2 and THP-1 macrophages and inhibition of the secretion of IL-8 and TNF-α in a DSS-induced colitis model mouse [63]. They suggested that tripeptide VPY from soy peptides may be promising for the treatment of IBD.

Insulin resistance and diabetes has revealed to relate closely between nutrient excess and activation of the innate immune system in most organs pertinent to energy homeostasis by the research for a mechanism linking the pathogenesis of obesity over the past two decades [64, 65, 66]. Inflammation has been revealed to occur as a consequence of obesity, and to play a causative role in generating insulin resistance, defective insulin secretion [i.e., MetS), and disruption of other aspects of energy homeostasis by recent many studies. It has been reported that the suppressive effect of soy protein on the progression of CKD/DN, which is highly related to MetS, is also exerted by the anti-inflammatory effect in renal tubules [67]. From such a close relationship between MetS and inflammation, it is easy to predict that the beneficial effect of soy protein on MetS may be due to its anti-inflammatory effect.

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3. Complementary effects of soy protein/peptide in combination with drugs

3.1 Effect of combined use with anti-hyperlipidaemic drugs

The mechanism by which soy protein lowers cholesterol differs from that of statins and fibrates. Soy protein lowers serum cholesterol levels by acting as a bile acid sequestrant, which binds bile in the gastrointestinal tract to prevent its reabsorption by performing the same anion exchange reaction as the resin cholestyramine [68, 69].

Statins and fibrates are drugs developed to improve blood lipid levels. Statins are known as the most efficient agents for reducing plasma cholesterol. Statins target hepatocytes and inhibit 3-hydroxy-3-methylglutaryl-coenzyme A [HMG-CoA] reductase in cholesterol metabolism. Accordingly, statin and soy protein are expected to act additively or synergistically to decrease cholesterol levels. There are known serious side effects from statins, including muscle symptoms, rhabdomyolysis [secondary renal failure due to destruction of specific muscle tissue], peripheral neuropathy, myopathy, liver dysfunction, and thrombocytopenia [70, 71, 72, 73]. Rhabdomyolysis often induces sudden kidney failure [74]. Fibrates, which are antagonists of peroxisome proliferator-activated receptor α [PPARα], are used in adjunct therapy for hypertriglyceridemia and are usually used in combination with statins. As fibrate-related side effects, the slight gastric region discomfort and myopathy [myalgia with increased creatinine phosphokinase] have been reported. In addition, increasing of the gallstones risk has been known by fibrates because of increasing of cholesterol in the bile duct. Use in combination of statins and fibrates is reported to even more raise the risk of rhabdomyolysis. So, combination use of these two agents is contraindicated in principle.

Nabiki et al. examined the effects of SPI on weight loss, markers of diabetes, and parameters of dyslipidaemia in obese diabetic patients by treated with statins and/or fibrates because of high levels of LDL cholesterol and triglycerides [75]. As a result, body weights of these patients decreased significantly by approximately 1 kg and their waist circumferences got thinner significantly by approximately 2 cm. Total cholesterol, triglyceride, LDL cholesterol, apolipoprotein B, and glycated hemoglobin levels of these patients decreased significantly, and HDL cholesterol levels increased significantly. In addition, a lipid metabolism-improving effect was also observed in patients who did not decrease weight. Therefore, it was suggested that the improving effect of lipid metabolism-related factors in these patients was not only due to weight loss but also a direct effect of soy protein. Use of soy protein may help to reduce the drug dose for dyslipidaemia. SPI is recommended for patients with mild dyslipidaemia prior to drug therapy or for maladaptive disease patients, such as those who have side effects from medications.

Combination prescription of fibrates and statins for patients with renal dysfunction and dyslipidaemia is contraindicated. Thus, physicians are unable to adequately treat lipid abnormalities for chronic kidney disease patients. It has been reported that when chronic kidney disease patients with dyslipidaemia ingested β-conglycinin, a major component of soy protein, for 3 months, triglyceride and LDL cholesterol levels improved. Renal function during the consumption period of β-conglycinin showed a tendency to improve despite protein intake [76]. β-conglycinin may help improve lipid abnormalities in patients with renal dysfunction as a complementary medical food material without decreasing kidney function. Moreover, β-conglycinin may improve renal dysfunction as a direct and/or secondary effect of ameliorating lipid abnormalities.

3.2 Concomitant effect with rheumatoid arthritis drug

Rheumatoid arthritis is due to inflammation triggered by an immune response to autoantigens. Many of these patients have swelling and pain due to polyarticular arthritis. Their pain interferes with activities of daily living [ADLs], such as cleaning, washing, dressing, and undressing. These patients are anxious for more comfortable ADLs with reduced pain. The mechanisms of onset of rheumatoid arthritis have been reported in many studies. Based on these results, numerous new therapeutic agents have been developed.

As a specific case of improved inflammation, outpatients with rheumatoid arthritis consumed soy peptide with therapeutic drugs and the levels of IL-6 and IL-1β were significantly lower in the soy peptide group than the placebo group [77]. An increase in blood IL-6 levels is associated with extra-articular symptoms of rheumatoid arthritis, such as general malaise, loss of appetite, weight loss, and a slight fever. The Disease Activity Score 28 [DAS 28, objective assessment of rheumatoid arthritis disease activity by physicians] and the Clinical Disease Activity Index [CDAI, patient’s own subjective indicator of rheumatoid arthritis disease activity] were calculated from the degree of ADLs’ improvement, the severity of pain, and subjective symptoms recorded by visual analogue scale [VAS]. The DAS 28 score of the peptide group was markedly decreased, and the CDAI of the peptide group was significantly lower than that in the placebo group.

These effects on cytokines were also evident in a cell experiment using articular chondrocytes from patients with rheumatoid arthritis [78]. In this in vitro cell study, treatment with soy peptide significantly suppressed the mRNA levels of MMP-3 and ADAMTS-4 enhanced by IL-1β stimulation. This finding also suggests that soy peptides may prevent the degradation of articular cartilage.

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4. Physiological effects of other legume proteins

Soy protein has excellent health benefits, but many soybeans grown in the world are genetically modified organisms [GMOs]. There is no problem with the safety of GMO soybeans. However, from the perspective of security, the use of soy protein in foods tends to be withheld. Recently, the use of pea and lupin proteins instead of soy protein has increased worldwide. Initially, pea protein was a substitute for soybean protein as an ingredient with physical characteristics functions, after that, its beneficial health function has been reported mainly in sports nutrition. Mung bean protein has a structure very similar to that of β-conglycinin. Mung bean protein has been reported to be responsible for the beneficial physiological functions reported for β-conglycinin.

4.1 Pea protein

Field pea [Pisum sativum L.] is grown in 84 different countries and constitutes the largest percentage [36%] of total pulse production worldwide [79]. Global pea production has continuously increased over the last 30 years. In 2008, field pea was cultivated on over 10 million hectares worldwide with a total world production of 12.13 million tons [80]. The top 5 countries for pea production are Canada, Russia, China, India and the USA. The global market for pea protein is expected to reach 34.8 million US dollars by 2020 [81]. The physical and chemical properties of pea protein can significantly influence its behaviors in food processing, storage and consumption [82, 83].

Life expectancy continues to increase worldwide. In the United States, adults 65 years of age and older are projected to more than double from 600 million to 1.6 billion worldwide between 2015 and 2050 [84]. Proper body composition, physical fitness, and a healthy appetite have been reported to lead to successful aging with higher performance [85, 86]. Skeletal muscle mass and strength begin to decline at age 30, and the rate of these losses accelerates at age 60 [87]. Protein ingestion strongly increases muscle protein synthesis rates [88]. Amino acids serve as precursors for de novo muscle protein synthesis and can act as strong signaling molecules activating translation initiation via the mechanistic/mammalian target of rapamycin complex-1 (mTORC1) pathway [89]. It was shown that BCAA ingestion increases myofibrillar protein synthesis rates during recovery from exercise only in young males [90]. Whey protein isolate [WPI] was used as the animal protein source because of its high concentration of BCAAs and its ability to increase satiety in response to a mixed meal [91]. While whey protein supplementation is known to enhance adaptations to resistance training, not all athletes are able or willing to consume whey or animal proteins. Vegetarian athletes who want to stick to a plant-based diet or those with restrictions on other animal foods often rely on other plant proteins as an equivalent alternative to whey protein [92]. Self-identify as vegetarian in just over 5% of U.S. adults aged 18–34 years and self-awareness as vegan in more than half of these respondents are reported in a 2016 Harris Poll conducted by the Vegetarian Resource Group [93]. Meat Free Mondays’ movement and an upsurge of plant-based protein food products in the marketplace strongly reflect the recent acceptance of these lifestyles [94].

Field pea contains a well-balanced amino acid profile [95]. Because of its availability, low cost, nutritional value and health benefits, pea protein has been widely used as a substitute for soybean or animal proteins in various functional applications [96, 97, 98, 99]. Pea protein can also be used as a nutritional supplement for sports and exercises. Pea protein is an excellent source of BCAAs and has high and balanced contents of leucine, isoleucine and valine. Indeed, there are reports that pea protein is as useful as whey protein in sports nutrition (Table 4) [100, 101, 102, 103].

Study titleDesign of studyNumber of subjectsDuration of studyOutcome [significant difference]Reference
Pea proteins oral supplementation promotes muscle thickness gains during resistance training: a double-blind, randomized, Placebo-controlled clinical trial vs. Whey protein.Randomized, Double-Blind, Placebo-Controlled Study16112-wkBiceps brachii muscle thickness↑[100]
The Effects of Whey vs. Pea Protein on Physical Adaptations Following 8-Weeks of High-Intensity Functional Training (HIFT): A Pilot Study.Randomized, Double-Blind, Placebo-Controlled Study158-wkResult of the resistance training program↑[101]
Effects of Whey and Pea Protein Supplementation on Post-Eccentric Exercise Muscle Damage: A Randomized Trial.Randomized, Double-Blind, Placebo-Controlled Study1095-dayCreatinine kinase↓, Myoglobin↓[102]
The Short-Term Effect of Whey Compared with Pea Protein on Appetite, Food Intake, and Energy Expenditure in Young and Older Men.Randomized, single-blinded crossover design30One shot [postprandial data]Appetite↑, Energy expenditure↑, 24-h energy intake↑[103]

Table 4.

Clinical studies of pea protein for sports nutrition.

Note: ↓ and ↑ signs represent decrease and increase, respectively, after supplement of active compounds [pea protein only or, pea and whey proteins].

In the future, pea protein is expected to be widely used as a sports nutritional supplement as well as a physical and functional ingredient in place of soybean protein.

4.2 Lupin protein

Lupin (Lupinus L.) is an ancient pulse “bean” crop, and in the new genus of modern agriculture, the lupin seeds have great potential for high-protein food, animal feed, food potential, soil fertility improvement, plants as cover crops, crop residues as stable feed, and soil improvement [104, 105]. Lupin is well known for its ability to fix nitrogen and grow on infertile soils, and is further known to be valuable in terms of cropping rotations during the growing season in agriculture with cereals, hay, oilseeds, beans of other legumes, and disease break crops for pasture [104, 106]. Wild indigenous lupins have bitter alkaloids. All modern species of L. angustifolius have total alkaloid levels in seeds of up to 200 mg/kg [0.02%] or less, which is 100 times lower than the seed alkaloid levels of nearly wild types. Compared to almost all food crops, lupins have only recently become of interest in modern crop breeding.

There has been considerable interest in lupin seeds recently, and as a human health food, the seeds are very high in dietary fiber, gluten-free, and virtually starch-free, and therefore have a very low glycemic impact [107]. What makes lupins even more valuable is that there are no genetically modified (GM) bean varieties under commercial cultivation. World production of lupin seed increased quickly in the 1970s and is dominated by Australian production.

Lupin seeds are high in protein, with levels similar to soybeans. Their grains are also known to be high in total dietary fiber, ~40 g/100 g dry matter, making lupins unique among ancient grains and beans. The main category of protein in lupin grains is globulin, with albumin making up the remainder. The major globulin categories are α-conglutin [35–37 g/100 g total protein], β-conglutin [44–45 g/100 g total protein], γ-conglutin [4–5 g/100 g total protein], and δ-conglutin [10–12 g/100 g total protein] [108, 109, 110, 111]. Nutritionally, the limiting amino acids in lupin protein are the sulfur-containing amino acids methionine and cysteine [112]. Compared to soy protein, which have a more complete essential amino acid profile, the lupin protein was reported to be slightly below the required level of sulfur-containing amino acids needed by adults [113]. However, Singla et al. reported that the sulfur-containing amino acid levels of lupin protein were similar to those of soy [114]. This discrepancy is probably due to differences in lupin protein varieties and production environments. Carvajal-Larenas et al. reviewed in detail the amino acid composition of whole lupin seeds and concluded that it varies slightly among species. In vitro digestibility is ~98% high for uncooked lupin protein and is similar to soybean [115].

In vitro models of Lupinus albus γ-conglutin have shown the biological activity that enhances insulin and metformin activity on intracellular glucose consumption, indicating the potential for regulation on blood glucose by γ-conglutin [116]. As a possible improvement of lipid metabolism, an increase in LDL receptor activity has been demonstrated by HepG2 cells [117]. Furthermore, isolated lupin proteins of have been reported to have hyperlipidemic, anti-atherogenic, and hypocholesterolemic effects in rabbits, rats, and chickens [118, 119]. Several clinical human studies have shown that lupin protein decreases total and LDL cholesterol, as well as triglyceride and reduce the glycaemic response (Table 5) [120, 121, 122, 123, 124, 125, 126, 127].

TitleDesign of studyNumber of total subjectsOutcome [significant difference]Reference
Lupin protein compared to casein lowers the LDL cholesterol: HDL cholesterol-ratio of hypercholesterolemic adultsRandomized, double-blind, placebo-controlled, parallel trial43Total-C↓, LDL-C↓, LDL: HDL-C ratio↓[120]
Hypocholesterolaemic effects of lupin protein and pea protein/fiber combinations in moderately hypercholesterolaemic individualsRandomized, double-blind, parallel group design175Total-C↓[121]
Lupin protein positively affects plasma LDL cholesterol and LDL:HDL cholesterol ratio in hypercholesterolemic adults after four weeks of supplementation: a randomized, controlled crossover studyRandomized, controlled, double-blind crossover study33 hypercholesterolemic subjectsLDL-C↓, HDL-C↑, LDL:HDL-C ratio↓[122]
Consuming a mixed diet enriched with lupin protein beneficiallyaffects plasma lipids in hypercholesterolemic subjects: A randomized controlled trialRandomized, controlled, double-blind three-phase crossover study72 hypercholesterolemic subjectsLDL-C↓, Homocysteine↓, TG↓, Uric acid↓[123]
Australian sweet lupin flour addition reduces the glycaemic index of a white bread breakfast without affecting palatability in healthy human volunteerRandomized, single-blind, cross-over design11 healthy subjectsPostprandial blood glucose↓[124]
Lupin and soya reduce glycaemia acutely in type 2 diabetesRandomized, cross-over trial24 diabetic adultsPostprandial blood glucose↓, Insulin response↑[125]
Hypoglycemic effect of lupin seed γ-conglutin in experimental animals and healthy human subjectsRandomized, double-blind, parallel group design15 adult healthy volunteersPostprandial blood glucose↓[126]
Short-Term Effects of Lupin vs. Whey Supplementation on Glucose and Insulin Responses to a Standardized Meal in a Randomized Cross-Over TrialRandomized, controlled, cross-over trial12 healthy male and female volunteersPostprandial blood glucose↓, Insulin response↑[127]

Table 5.

Clinical studies of lupin protein on improving lipid and glucose metabolisms.

Note: ↓ and ↑ signs represent decrease and increase, respectively, after supplement of active compounds. Total-cholesterol (Total-C); low-density lipoprotein cholesterol (LDL-C); triglyceride (TG); high-density lipoprotein cholesterol (HDL-C).

In general, the anti-nutrient factor of lupins is considered to be low compared to other legumes such as soybeans. Specifically, protease inhibitors are present at very low levels and are of minor importance in lupin crops. Trypsin inhibitor activity is described as “negligible” in Lupinus species, “very strong” at 43–84 trypsin inhibitor units [TIU/mg] in soybeans, and high [17–51 TIU/mg] in common beans [128]. Bitter lupin seed varieties contain quinolizine alkaloids, which may be toxic to humans. These toxic effects were recently reviewed by Carvajal-Larenes et al. [115]. Therefore, its maximum legal level of 0.02 g/100 g lupine powder and food has been legislated in several countries. There were no differences in alkaloids in grains among commercial L. angustifolius cultivars from western Australia in the same region and season, and all samples were below the levels permitted for maximum human food use.

Lupin protein, a legume, is a plant protein with similar attributes to soybean protein [129] and can be a substitute for soybean in the food industry [130, 131]. Further extensive research is expected due to the need for alternatives to animal proteins.

4.3 Mung bean protein

The mung bean (Vigna radiata L.) is one of the most important edible legume crops, grown on more than 6 million ha worldwide (approximately 8.5% of the global pulse area) and consumed by most households in Asia [132]. For individuals who cannot afford animal proteins or those who are vegetarian, mung bean is comparatively low cost and is a good source of protein. Furthermore, mung bean protein is more easily digestible than protein in other legumes [133]. In addition to the nutritional properties of mung bean, it has been known that mung beans have various physical regulation functions from ancient times. In the Compendium of Materia Medica (the “Bencao Gangmu”), a well-known Chinese pharmacopeia, mung beans have recorded to be utilized as a traditional Chinese medicine for its detoxification activities, recuperation of mentality, ability to alleviate heat stroke, and regulation of gastrointestinal upset.

Mung bean protein isolate (MuPI) dose-dependently reduced plasma lipid levels, such as total cholesterol, triglycerides, and non-high-density lipoprotein cholesterol [non-HDL-C] in hamsters [134, 135]. The mechanism underlying the cholesterol-lowering activity of mung bean protein was speculated to increase fecal bile acid and sterol excretion and decrease cholesterol absorption and synthesis. This mechanism is the same as that reported for SPI [68, 69]. In another study, MuPI was found to lower blood triglyceride levels in normal rats by inducing adiponectin and reducing triglyceride synthesis via insulin signaling [136]. This mechanism is the same as that reported for β-conglycinin [23]. From these findings, MuPI can be expected to be more effective in improving lipid metabolism. The main component of MuPI, accounting for over 80% of the protein, is 8S globulin. 8S globulin exhibited the highest degree of sequence identity [68%] and structural similarity with β-conglycinin [137, 138]. MuPI is expected to exhibit a four times stronger beneficial function on human health than SPI, in which β-conglycinin accounts for only 20% of the total protein.

The positive effects of MuPI on glucose metabolism in pre-diabetes patients was confirmed. In recent double-blind, placebo-controlled clinical trial, the test group subjects were instructed to consume a total of 2.5 g of MuPI twice daily for 12 weeks, with pre-diabetes [fasting plasma glucose level of 110–125 mg/dL or 2-h plasma glucose level of 140–200 mg/dL by the 75-g glucose tolerance test]. In this study, MuPI was shown to suppress to increase fasting plasma glucose and insulin levels compared to the placebo group. Triglyceride levels significantly decreased in subjects with hyperlipidaemia [139]. Another double-blind, placebo-controlled clinical trial of 44 healthy subjects showed that after consumption of 3.0 g/d MuPI for 8 weeks, insulin levels and homeostatic model assessment of insulin resistance values significantly decreased, and plasma glucose levels showed a downtrend, although it was not significant [140]. The lack of a beneficial effect of MuPI on blood glucose concentrations may be attributed to the exclusion of volunteers with abnormal blood glucose concentrations in this study. In this study, the body compositions of subjects were measured by dual-energy X-ray absorptiometry. As a result, a decrease in body fat mass and an increase in lean body mass in the test group were revealed. Conversely, in the control group, body fat mass increased and lean body mass decreased. The differences in body fat mass and lean body mass within each group and between the test and control groups were not statistically significant. However, the adiponectin level in the test group significantly increased, and it decreased in the control group. There was a significant difference between the net changes in the test and control groups [140]. These findings indicate that MuPI might improve insulin sensitivity by decreasing the accumulation of visceral fat.

Non-alcoholic fatty liver disease [NAFLD] represents a spectrum of liver diseases involving hepatocyte dysfunction caused by hepatic triglyceride accumulation in these cells. The prevalence of NAFLD has increased with the increased prevalence of obesity and metabolic syndrome. NAFLD is now a common disease, affecting 30% of the US population and 20% of Asian and European populations [141]. Rodent studies have shown that SPI intake reduces hepatic triglyceride accumulation [142, 143]. The detailed mechanism underlying the hepatic triglyceride-reducing effect of SPI remains to be elucidated, but β-conglycinin is likely to play an important role [135]. Indeed, the administration of purified β-conglycinin results in an even stronger reduction in hepatic triglycerides than SPI administration [18, 144]. From these results, it is expected that MuPI also has a preventive effect on NAFLD by preventing hepatic triglyceride accumulation. The effect of MuPI on hepatic triglyceride accumulation elucidated the potential ability of MuPI to prevent NAFLD onset and progression in experiments using an atherogenic diet-induced NASH mouse model in mice fed a normal-fat or high-fat diet [145]. In the abovementioned clinical trial [140], Alanine aminotransferase [ALT] levels increased slightly in the control group, whereas significantly decreased in the test group. Of the blood test items, ALT is one of important indicators of the degree of liver dysfunction.

The released peptides obtained from mung bean protein hydrolysate may exhibit bioactivity as angiotensin I-converting enzyme (ACE) inhibitors, antioxidants, and anti-cancer Asiatic acid carriers due to their sequence characteristics [146, 147]. A peptide [<3 kDa], with a small molecular weight isolated from MuPI hydrolysates, was reported to show high ACE inhibitory and antioxidant activities, including DPPH radical scavenging activity, hydroxyl radical scavenging ability, and metal-chelating activity [146]. Three kinds of novel peptides exerting high ACE inhibitory activity were isolated from the alcalase hydrolysate of MuPI, and the amino acid sequences of these peptides were identified to be Lys-Asp-Tyr-Arg-Leu, Val-Thr-Pro-Ala-Leu-Arg, and Lys-Leu-Pro-Ala-Gly-Thr-Leu-Phe [148].

The relationships between MuPI intake, strength, and lean body mass (LBM) in underactive vegetarians were examined, and the impact of MuPI supplementation on these indices was recorded utilizing an eight-week, randomized, controlled feeding trial. LBM significantly correlated with grams of protein consumed daily and was also significantly correlated with grip strength and lower body strength [149]. Mung beans are inadequate in threonine, tryptophan, and the sulfur-containing amino acids cysteine and methionine, but they contain high levels of essential amino acids, notably leucine, lysine, and phenylalanine [150]. Although it is necessary to consider the amino acid balance, it is expected that MuPI will be widely used in the field of sports nutrition in the future.

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5. Conclusion

If the current pace of population growth continues, the global population is expected to surpass 9 billion by 2050. In addition to this increase in population, the change of dietary habits of emerging countries due to their increased GDP will require, in 2050, we will need twice as much protein as we had in 2005.

So far, we have been able to meet the increasing demand for protein by improving the productivity of agriculture. However, in the future, this growth alone will not be enough to absorb the increase, and the balance between supply and demand will begin to collapse as early as 2030. This prediction is called the “protein crisis,” and has recently begun to attract attention, especially in Europe and the United States. To solve this protein crisis, it is essential to use highly productive plant proteins as food ingredients instead of animal proteins, which are less efficient in production.

WHO has called for the need to address the double burden of malnutrition. This means that we need to look not only at nutrient deficiencies, but also at nutrient excesses. Obesity caused by over-nutrition and the resulting lifestyle-related diseases are spreading around the world. In this regard, consumer demand for plant protein-based products is high and expected to grow considerably in the next decade. A variety of soy and other plant-based functional foods have been recommended by many health organizations worldwide.

Currently, contributions to the SDGs (Sustainable Developing Goals) are being appealed around the world. There is widespread recognition that the replacement of animal protein with vegetable protein not only contributes to human health, but also to the earth health. Wider and prudent use of plant proteins in the diet can help to supply adequate high-quality protein for the population and may reduce the potential for adverse environmental consequences.

This chapter focused on the recently reported physiological functions of legumes-derived plant proteins, including soybeans. Further research is expected to lead to more widely use of the legumes introduced in this chapter and to the discovery and use of legumes with new functionalities.

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Acknowledgments

Of the research results presented in this chapter, our own research results were achieved in the laboratory at Fuji Oil Co. Ltd. and/or Fuji Oil Holdings Inc. to which I belonged until March of this year from 1986. I believe that I could not have done this without the cooperation of the researchers who belonged to that laboratory. I would like to take this opportunity to express my deepest gratitude.

References

  1. 1. Food and Drug Administration: Food labelling health claims; soy protein and coronary heart disease. Fed Regist. 1999:64:57700-57733
  2. 2. Alberti KG, Zimmer PZ: Definition, diagnosis and classification of diabetes mellitus and its complication, part 1: Diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med. 1998:15:539-553
  3. 3. National Cholesterol Education Program (NCEP): Expert panel on detection and treatment on high blood cholesterol in adults: Third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (adult treatment panel III) final report. Circulation. 2002:106:3143-3421
  4. 4. Grundy SM, Cleeman JI, Daniels SR, Donato KA, Eckel RH, Franklin BA, Gordon DJ, Krauss RM, Savage PJ, Smith SC Jr, Spertus JA, Costa F, American Heart Association; National Heart, Lung, and Blood Institute: Diagnosis and management of the metabolic syndrome: An American Heart Association/National Heart, lung and blood institute scientific statement. Circulation. 2005:112:2735-2752
  5. 5. Zimmet P, Magliano D, Matsuzawa Y, Alberti G, Shaw J: The metabolic syndrome: A global public health problem and a new definition. J Atheroscle. Thromb. 2005:12:295-300
  6. 6. Reaven GM: The metabolic syndrome: Is this diagnosis necessary? Am J Clin Nutr. 2006:83:1237-1247
  7. 7. The Examination Committee of Criteria for `Obesity Disease` in Japan: New criteria for `obesity disease` in Japan: Circulation J. 2006:66:987-992
  8. 8. Definition and the diagnostic standard for metabolic syndrome Committee to Evaluate Diagnostic Standards for Metabolic Syndrome: Nippon Naika Gakkai Zasshi. 2005:94:794-809 (in Japanese)
  9. 9. Anderson JW, Johnstone BM and Cook-Newell MF: Meta-analysis of the effects of soy protein intake on serum lipids. N Eng J Med. 1995:333:276-282
  10. 10. Zhan S and Ho SC: Meta-analysis of the effects of soy protein containing isoflavones on the lipid profiles. Am J Clin Nutr. 2005:81:397-408
  11. 11. Reynolds K, Chin A, Lees KA, Nguyen A, Bujnowski D, He J: A meta-analysis of the effect of soy protein supplementation on serum lipids. Am J Cardiol. 2006:98(5):633-640
  12. 12. Sirtori CR, Eberini I, Arnoldi A: Hypocholesterolaemic effects of soya proteins: Result of recent studies are predictable from Anderson meta-analysis data. Brit J Nutr. 2007:97:816-822
  13. 13. Anderson JW, Bush HM: Soy protein effects on serum lipoproteins: A quality assessment and meta-analysis of randomized, controlled studies. J Am Coll Nutr. 2011:30(2):79-91
  14. 14. Li SS, Mejia SB, Lytvyn L, Stewart SE, Viguiliouk E, Ha V, de Souza RJ, Leiter LA, Kendall CWC, Jenkins DA, Sievenpiper JL: Effect of plant protein on blood lipids: A systematic review and meta-analysis of randomized controlled trials. J Am Heart Assoc. 2017:6:e006659
  15. 15. Mejia SB, Messina M, Li SS, Viguiliouk E, Chiavaroli L, Khan TA, Srichaikul K, Mirrahimi A, Sievenpiper JL, Kris-Etherton P, Jenkins DJA: A meta-analysis of 46 studies identified by the FDA demonstrates that soy protein decreases circulating LDL and Total cholesterol concentrations in adults. J Nutr. 2019:149:968-981
  16. 16. Moradi M, Daneshzad E, Azadbakht L: The effects of isolated soy protein, isolated soy isoflavones and soy protein containing isoflavones on serum lipids in postmenopausal women: A systematic review and meta-analysis. Crit Rev Food Sci Nutr. 2020:60(20):3414-3428
  17. 17. Samoto M, Maebuchi M, Miyazaki C, Kugitani H, Kohno M, Hirotsuka M, Kito M: Abundant proteins associated with lecithin in soy protein isolate. Food Chem. 2007:102(1):317-322
  18. 18. Aoyama T, Kohno M, Saito T, Fukui K, Takamatsu K, Yamamoto T, Hashimoto Y, Kito M: Reduction by phytate-reduced soybean β-conglycinin of plasma triglyceride level of young and adult rats. Biosci Biotechnol Biochem 2001:65:1071-1075
  19. 19. Moriyama T, Kishimoto K, Nagai K, Urade R, Ogawa T, Utsumi S, Maruyama N, Maebuchi M: Soybean β-conglycinin diet suppresses serum triglyceride levels in normal and genetically obese mice by induction of β-oxidation, downregulation of fatty acid synthase and inhibition of triglyceride absorption. Biosci Biotech Biochem 2004:68(2):352-359
  20. 20. Fukui K, Kojima M, Tachibana N, Kohno M, Takamatsu K, Hirotsuka M, Kito M: Effects of soybean β-conglycinin on hepatic lipid metabolism and fecal lipid excretion in normal adult rats. Biosci Biotech Biochem 2004:68(5):1153-1155
  21. 21. Kohno M, Hirotsuka M, Kito M and Matsuzawa Y: Decrease in serum triacylglycerol and visceral fat mediated by dietary soybean β-conglycinin. J Atheroscler Thromb. 2006:13:247-255
  22. 22. Baba T, Ueda A, Kohno M, Fukui K, Miyazaki C, Hirotsuka M, Ishinaga M: Effects of soybean beta-conglycinin on body fat ratio and serum lipid levels in healthy volunteers of female university students. J Nutr Sci Vitaminol (Tokyo). 2004:50(1):26-31
  23. 23. Tachibana N, Iwaoka Y, Hirotsuka M, Horio F, Kohno M: Beta conglycinin lowers very-low-density lipoprotein-triglyceride levels by increasing adiponectin and insulin sensitivity in rats. Biosci Biotechnol Biochem 2010:74(6):1250-1255
  24. 24. Ohara S, Matsui Y, Tamesada M, Saitou M, Komatsu R, Yoshino T, Tsubaki T, Itakura H: Serum triacylglycerol-lowering effect of soybean b-conglycinin in mildly hypertriacylglycerolemic individuals. e-SPEN, Eur e-Journal Clin Nutr Met. 2007:2:12-16
  25. 25. Ma D, Taku K, Zhang Y, Jia M, Wang Y, Wang P: Serum lipid-improving effect of soyabean b-conglycinin in hyperlipidaemic menopausal women. Brit J Nutr 2013:110:1680-1684
  26. 26. Nishimura M, Ohkawara T, Sato Y, Satoh H, Takahashi Y, Hajika M, Nishihira J: Improvement of triglyceride levels through the intake of enriched-β-Conglycinin soybean (Nanahomare) revealed in a randomized, double-blind, Placebo-Controlled Study. Nutrients 2016:8:491
  27. 27. , Shimizu K, Wada S, Nirengi S, Kataoka H, Higashi A: Effects of beta-conglycinin intake on circulating FGF21 levels and brown adipose tissue activity in Japanese young men: a single intake study and a randomized controlled trial. J Physiol Anthropol. 2020:39(1):17
  28. 28. Hill NR, Fatoba ST, Oke JL, Hirst JA, O’Callaghan CA, Lasserson DS, Hobbs FD: Global prevalence of chronic kidney disease – A systematic review and meta-analysis. PLoS One. 2006:11(7):e0158765
  29. 29. GBD 2015 Mortality and causes of death collaborators: Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980-2015: A systematic analysis for the global burden of disease study 2015. Lancet. 2016:388:1459-1544
  30. 30. Sun X, He J, Ji XL, Zhao YM, Lou HY, Song XX, Lou HY, Song XX, Shan LZ, Kang YX, Zeng WH, Pang XH, Zhang SZ, Ding Y, Ren YZ, Shan PF: Association of chronic kidney disease with coronary heart disease and stroke risks in patients with type 2 diabetes mellitus: An observational cross-sectional study in Hangzhou, China. Chin Med J. 2017:130:57-63
  31. 31. Hoogeveen EK, Geleijnse JM, Giltay EJ, Soedamah-Muthu SS, De Goede J, Oude Griep LM, Stijnen T, Kromhout: Kidney function and specific mortality in 60-80 years old post-myocardial infarction patients: A 10-year follow-up study. PLoS One 2017:12(2):e0171868
  32. 32. Agrawal V, Shah A, Rice C, Franklin BA, McCullough PA: Impact of treating the metabolic syndrome on chronic kidney disease. Nat Rev Nephrol. 2009:5:520-528
  33. 33. Yokoyama H, Kawai K, Kobayashi M, Japan diabetes clinical data management study group: Microalbuminuria is common in Japanese type 2 diabetic patients: A nationwide survey from the Japan diabetes clinical data management study group (JDDM 10). Diabetes Care 2007:30(4):989-992
  34. 34. Parving HH, Lewis JB, Ravid M, Remuzzi G, Hunsicker LG, DEMAND investigators: Prevalence and risk factors for microalbuminuria in a referred cohort of type II diabetic patients: A global perspective. Kidney Int. 2006:69:2057-2063
  35. 35. van Westing AC, Küpers LK, Geleijnse JM: Diet and Kidney Function: A Literature Review. Curr Hypertens Rep. 2020:22:14
  36. 36. Haring B, Selvin E, Liang M, Coresh J, Grams M, Petruski-Ivleva N, Steffen LM, Rebholz CM: Dietary protein sources and risk for incident chronic kidney disease: Results from the atherosclerosis risk in communities (ARIC) study. J Ren Nutr. 2017:27:233-242
  37. 37. Asanoma M, Tachibana N, Hirotsuka M, Kohno M, Watanabe Y: Effects of soy protein isolate feeding on severe kidney damage in DOCA salt-treated obese Zucker rats. J Agric Food Chem. 2012:60:5367-5372
  38. 38. Jheng HG, Hirotsuka M, Goto Y, Shibata M, Matsumura Y, Kawada T: Dietary low-fat soy milk powder retards diabetic nephropathy progression via inhibition of renal fibrosis and renal inflammation. Mol Nutr Food Res. 2016:61(3):e2001600461
  39. 39. Azadbakht L, Atbak S, Esmaillzadeh A: Soy protein intake, cardiorenal indices, and c-reactive protein in type 2 diabetes with nephropathy. Diabetes Care 2008:31:648-654
  40. 40. Zhang J, Liu J, Su J, Tian F: The effects of soy protein on chronic kidney disease: A meta-analysis of randomized controlled trials. Eur J Clin Nutr 2014:68:987-993
  41. 41. McGraw NJ, Krul ES, Grunz-Borgmann E, Parrish AR: Soy-based renoprotection. World J Nephrol. 2016:5(3):233-257
  42. 42. Jing Z, Wei-Jie Y: Effects of soy protein containing isoflavones in patients with chronic kidney disease: A systematic review and meta-analysis. Clin Nutr. 2016:35(1):117-124
  43. 43. Rafieian-Kopaei M, Beigrezaei S, Nasri H, Kafeshani M: Soy protein and chronic kidney disease: An updated review. Int J Prev Med. 2017:8:105
  44. 44. Pan Y, Guo LL, Jin HM: Low-protein diet for diabetic nephropathy: A meta-analysis of randomized controlled trials. Am J Clin Nutr. 2008:88:660-666
  45. 45. Koya D, Haneda M, Inomata S, Suzuki Y, Suzuki D, Makino H, Shikata K, Murakami Y, Tomino Y, Yamada K, Araki SI, Kashiwagi A. Kikkawa R, Low-protein diet study group: Long-term effect of modification of dietary protein intake on the progression of diabetic nephropathy: A randomized control trial. Diabetologia. 2009:52(10):2037-2045
  46. 46. Rughooputh MS, Zeng R, Yao Y: Protein diet restriction slows chronic kidney disease progression in non-diabetic and in type 1 diabetic patients, but not in type 2 diabetic patients: A meta-analysis of randomized controlled trials using glomerular filtration rate as a surrogate. PLoS One. 2015:10(12):e0145505
  47. 47. Artis D, Spits H: The biology of innate lymphoid cells. Nature. 2015:517:293-301
  48. 48. Pedraza-Alva G, Pérez-Martínez L, Valdez-Hernández L, Meza-Sosa K.F, Ando-Kuri M: Negative regulation of the inflammasome: Keeping inflammation under control. Immunol Rev. 2015:265:231-257
  49. 49. Fernandes JV, Cobucci RN, Jatobá CA, Fernandes TA, de Azevedo JW, de Araújo JM: The role of the mediators of inflammation in cancer development. Pathol Oncol Res. 2015:21:527-534
  50. 50. Heppner FL, Ransohoff RM, Becher B: Immune attack: The role of inflammation in Alzheimer disease. Nat Rev Neurosci. 2015:16:358-372
  51. 51. Waisman A, Liblau RS, Becher B: Innate and adaptive immune responses in the CNS. Lancet Neurol. 2015:14:945-955
  52. 52. Singh BP, Vij S, Hati S: Functional significance of bioactive peptides derived from soybean. Peptides 2014:54:171-179
  53. 53. Jeong HJ, Park JH, Lam Y, de Lumen BO: Characterization of lunasin isolated from soybean. J Agric Food Chem. 2003:51:7901-7906
  54. 54. Hernández-Ledesma B, Hsieh CC, de Lumen BO: Lunasin, a novel seed peptide for cancer prevention. Peptides. 2009:30:426-430
  55. 55. Shidal C, Al-Rayyan N, Yaddanapudi K, Davis KR: Lunasin is a novel therapeutic agent for targeting melanoma cancer stem cells. Oncotarget. 2016:7:84128-84141
  56. 56. Lam Y, Galvez A, de Lumen BO: Lunasin suppresses E1A-mediated transformation of mammalian cells but does not inhibit growth of immortalized and established cancer cell lines. Nutr Cancer. 2003:47:88-94
  57. 57. Lule VK, Garg S, Pophaly SD, Hitesh, Tomar SK: Potential health benefits of lunasin: A multifaceted soy-derived bioactive peptide. J Food Sci. 2015:80:R485–R494
  58. 58. Tsuruki T, Kishi K, Takahashi M, Tanaka M, Matsukawa T, Yoshikawa M: Soymetide, an immunostimulating peptide derived from soybean beta-conglycinin, is an fMLP agonist. FEBS Lett. 2003:540:206-210
  59. 59. Draganidis D, Karagounis LG, Athanailidis I, Chatzinikolaou A, Jamurtas AZ, Fatouros IG: Inflammaging and skeletal muscle: Can protein intake make a difference? J Nutr. 2016:146:1940-1952
  60. 60. Dia VP, Bringe NA, de Mejia EG: Peptides in pepsin-pancreatin hydrolysates from commercially available soy products that inhibit lipopolysaccharide-induced inflammation in macrophages. Food Chem. 2014:152:423-431
  61. 61. Tontini GE, Vecchi M, Pastorelli L, Neurath MF, Neumann H: Differential diagnosis in inflammatory bowel disease colitis: State of the art and future perspectives. World J Gastr. 2015;21(1):21-46
  62. 62. Young D, Ibuki M, Nakamori T, Fan M, Mine Y: Soy-derived di- and tripeptides alleviate colon and ileum inflammation in pigs with dextran sodium sulfate-induced colitis. J Nutr. 2012:142:363-368
  63. 63. Kovacs-Nolan J, Zhang H, Ibuki M, Nakamori T, Yoshiura K, Turner PV, Matsui T, Mine Y: The PepT1-transportable soy tripeptide VPY reduces intestinal inflammation. Biocim Biophys Acta. 2012:1820:1753−1763
  64. 64. Hotamisligil GS: Inflammation and metabolic disorders. Nature. 2006:444(7121):860-867
  65. 65. Lumeng CN, Saltiel AR: Inflammatory links between obesity and metabolic disease. J Clin Invest. 2011:121(6):2111-2117
  66. 66. Lackey DE, Olefsky JM: Regulation of metabolism by the innate immune system. Nat Rev Endocrinol. 2016:12(1):15-28
  67. 67. Ohta S, Asanoma S, Irie N, Tachibana N and Kohno M: Soy phospholipids exert a renoprotective effect by inhibiting the nuclear factor kappa B pathway in macrophages. 2021. in preparation
  68. 68. Sugano, M, Yamada Y, Yoshida K, Hashimoto Y, Matsuo T, Kimoto M: The hypocholesterolemic action of the undigested fraction of soybean protein in rats. Atherosclerosis. 1988:72:115-122
  69. 69. Sugano M, Goto S, Yamada Y, Yoshida K, Hashimoto Y, Matsuo T, Kimoto M: Cholesterol-lowering activity of various undigested fractions of soybean protein in rats. J Nutr., 1990:120(9):977-985
  70. 70. Kwak H-B: Statin-induced myopathy in skeletal muscle: The role of exercise. J Lifestyle Med. 2014:4(2):71-79
  71. 71. Lei Q, Peng WN, You H, Hu ZP, Lu W.: Statins in nervous system-associated disease: Angels or devils? Pharmazie. 2014:69(6):448-454
  72. 72. Laufs U, Scharnagi H, Halle M, Windler E, Endres M, Marz W: Treatment options for statin-associated muscle symptoms. Dtsch Arztebi Int. 2015:112:748-755
  73. 73. Haque T, Sasatomi E, Hayashi PH: Drug-induced liver injury: Pattern recognition and future directions. Gut Liver. 2016:10(1):27-36
  74. 74. Ambapkar SN, Shetty N, Dwivery A, Malve HO: Statin-induced rhabdomylysis in patients with renal failure and underlying undiagnosed hypothyroidism. Indian J Crit Care Med. 2016:20(5):305-307
  75. 75. Nabiki J, Yoshida M, Maeda A, Yamauchi F, Asayama S, Kohno M, Suzuki M, Harano A, Harano Y: Utility and significance of isolated soy protein snack for obesity and dyslipidemia. J Jpn Soc Clin Nutr. 2016:38(2):85-95 (in Japanese)
  76. 76. Ueda F, Ohtoshi K, Kohno M, Harano Y: Effects and clinical significances of oral supplementary soybean β-conglycinin in subjects with dyslipidemia and renal dysfunction. 2021. in preparation
  77. 77. Yoshioka H, Yui N, Maegawa K, Kohno M, Misuya H and Yudoh K: Assessment of the effect of soy peptide in joint symptoms of rheumatoid arthritis patients. Pharmacometrics, 2015:88(3/4):39-46 (in Japanese)
  78. 78. Arito M, Mitsui H, Kurokawa MS, Yudoh K, Kamada T, Niki H, Kato T: Effects of soy peptides on IL-1β-induced matrix-degrading enzymes in human articular chondrocytes. Integr Mol Med, 2016:3(3):661-665
  79. 79. Dahl WJ, Foster LM, Tyler RT: Review of the health benefits of peas (Pisum sativum L.). Brit J Nutr. 2012:108(S1):S3–S10
  80. 80. Schatz B, G Endres: Field pea production. NDSU Extension Service. 2009:A-1166 (Revised):1-8
  81. 81. Grand View Research 2015. Pea protein market by type (isolates, concentrates, textured), application, textured pea protein by form (dry, wet), by region – Global trends and forecast to 2020. http://www.researchandmarkets.com/research/b8x63n/pea_protein
  82. 82. Shevkani K, Singh N, Kaur A, Rana JC: Structural and functional characterization of kidney bean and field pea protein isolates: A comparative study. Food Hydrocol. 2015:43:679-689
  83. 83. Lu ZX, He JF, Zhang YC, Bing DJ: Composition, physicochemical properties of pea protein and its application in functional foods. Crit Rev Food Sci Nutr. 2020:60(15):2593-2605
  84. 84. Roberts AW, Ogunwole SU, Blakeslee L, Rabe MA, editors. The population 65 years and older in the United States 2016: US Department of Commerce Washington (DC); 2018. United States Census Bureau
  85. 85. Cosco TD, Prina AM, Perales J, Stephan BC, Brayne C: Operational definitions of successful aging: A systematic review. Int Psychogeriatr 2014:26:373-381
  86. 86. Anton SD, Woods AJ, Ashizawa T, Barb D, Buford TW, Carter CS, Clark DJ, Cohen RA, Corbett DB, Cruz-Almeida Y, Dotson V, Ebner N, Efron PA, Fillingim RB, Foster TC, Gundermann DM, Joseph A-M, Karabetian C, Leeuwenburgh C, Manini TM, Marsiske M, Mankowski RT, Mutchie HL, Perri MG, Ranka S, Rashidi P, Sandesara B, Scarpace PJ, Sibille KT, Solber LM, Someya S, Uphold C, Wohlgemuth S, Wu SS, Pahor M: Successful aging: advancing the science of physical independence in older adults. Ageing Res Rev. 2015:24:304-327
  87. 87. Liguori I, Russo G, Aran L, Bulli G, Curcio F, Della-Morte D, Gargiulo G, Testa G, Cacciatore F, Bonaduce D, Abete P: Sarcopenia: Assessment of disease burden and strategies to improve outcomes. Clin Interv Aging. 2018:13:913-927
  88. 88. Rennie MJ, Bohe J, Wolfe RR: Latency, duration and dose response relationships of amino acid effects on human muscle protein synthesis. J Nutr, 2002:132:3225S–3227S
  89. 89. Kimball SR, Jefferson LS: Signalling pathways and molecular mechanisms through which branched-chain amino acids mediate translational control of protein synthesis. J Nutr. 2006:136:227S–231S
  90. 90. Jackman SR, Witard OC, Philp A, Wallis GA, Baar K, Tipton KD: Branched-chain amino acid ingestion stimulates muscle myofibrillar protein synthesis following resistance exercise in humans. Front Physiol. 2017:8:390. DOI: 10.3389/fphys.2017.00390
  91. 91. Hall WL, Millward DJ, Long SJ, Morgan LM: Casein and whey exert different effects on plasma amino acid profiles, gastrointestinal hormone secretion and appetite. Br J Nutr. 2003:89:239-248
  92. 92. Fuhrman J, Ferreri DM: Fueling the vegetarian (vegan) athlete. Curr Sports Med Rep. 2010:9:233-241
  93. 93. How Many Adults in the U.S. Are Vegetarian and Vegan? 2019. Available from https://www.vrg.org/nutshell/Polls/2016_adults_veg.htm
  94. 94. De Backer CJS, Hudders L: From meatless Mondays to meatless Sundays: Motivations for meat reduction among vegetarians and semi-vegetarians who mildly or significantly reduce their meat intake. Ecol Food Nutr. 2014:53:639-657
  95. 95. Nunes MC, Raymundo A, Sousa I: Rheological behaviour and microstructure of pea protein/k-carrageenan/starch gels with different setting conditions. Food Hydrocol, 2006:20(1):106-113
  96. 96. Iqbal A, Khalil IA, Ateeq N, Khan MS: Nutritional quality of important food legumes. Food Chem 2006:97(2):331-335
  97. 97. Maninder K, Sandhu KS, Singh N: Comparative study of the functional, thermal and pasting properties of flours from different field pea (Pisum sativum L.) and pigeon pea (Cajanus cajan L.) cultivars. Food Chem. 2007:104(1):259-267
  98. 98. Aluko RE, Mofolasayo OA, Watts BM: Emulsifying and foaming properties of commercial yellow pea (Pisum sativum L.) seed flours. J Agri Food Chem, 2009:57(20):9793-9800
  99. 99. Barac, M, Cabrilo S, Pesic M, Stanojevic S, Zilic S, Macej O, Ristic N: Profile and functional properties of seed proteins from six pea (Pisum sativum) genotypes. Intr J Mol Sci 2010:11(12):4973-4990
  100. 100. Babault N, Païzis1 C, Deley G, Guérin-Deremaux L, Saniez M-H, Lefranc-Millot C, Allaert FA: Pea proteins oral supplementation promotes muscle thickness gains during resistance training: a double-blind, randomized, placebo-controlled clinical trial vs. whey protein. J Inter Soc Sports Nutr. 2015:12:3
  101. 101. Banaszek A, Townsend JR, Bender D, Vantrease WC, Marshall AC, Johnson KD: The effects of whey vs. pea protein on physical adaptations following 8-weeks of high-intensity functional training (HIFT): A pilot study. Sports. 2019:7:12
  102. 102. Nieman DC, Zwetsloot KA, Simonson AJ, Hoyle AT, Wang X, Nelson HK, Lefranc-Millot C, Guérin-Deremaux L: Effects of whey and pea protein supplementation on post-eccentric exercise muscle damage: A randomized trial. Nutrients. 2020:12:2382
  103. 103. Hawley AL, Gbur E, Tacinelli AM, Walker S, Murphy A, Burgess R, Baum BI: The short-term effect of whey compared with pea protein on appetite, food intake, and energy expenditure in young and old men. Curr Dev Nutr. 2020:4:nzaa009
  104. 104. Gladstones JS, Lupins as Crop Plants: Biology, Production and Utilization. edited by Gladstones JS, Atkins CA, Hamblin J: 1998. Wallingford, UK: CAB INTERNATIONAL, pp. 465
  105. 105. Lamber H, Clements JC, Nelson MN: How a phosphorus-acquisition strategy based on carboxylate exudation powers the success and agronomic potential of lupines (Lupinus, Fabaceae). Am J Bot. 2013:100(2):263-288
  106. 106. Seymour M, Kirkegaard J, Peoples M, White P, French R: Break-crop benefits to wheat in Western Australia – Insights from over three decades of research. Crop Pasture Sci. 2012:63(1). DOI:10.1071/cp11320
  107. 107. Dove ER, Mori TA, Chew GT, Barden AE, Woodman RJ, Puddey IB, Sipsas S, Hodgson JM: Lupin and soya reduce glycaemia acutely in type 2 diabetes. Br J Nutr. 2011:106(7):1045-1051
  108. 108. Blagrove RJ, Gillespie JM: Isolation, purification and characterization of the seed globulins of Lupinus angustifolius. Australian J Plant Physiol. 1975:2(1):13-27
  109. 109. Duranti M, Consonni A, Magni C, Sessa F, Scarafoni A: The major proteins of lupin seed: Characterization and molecular properties for use as functional and nutraceutical ingredients. Trends Food Sci Tech. 2008:19:624-633
  110. 110. Foley RC, Jimenez-Lopez JC, Kamphuis LG, Hane JK, Melser Su, Singh KB: Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches. BMC Plant Biology. 2015:15:106. DOI 10.1186/s12870-015-0485-6
  111. 111. Patterson DS. Composition and food uses of Lupins. In: Gladstones JS, Atkins CA, Hamblin J, editors. Lupins as a Crop Plants: Biology, Production and Utilization: CAB International London; 1998. p.353-384
  112. 112. Gulewicz P, Martı’nez-Villaluenga C, Frias J, Ciesiołka D, Gulewicz K, Vidal-Valverde C: Effect of germination on the protein fraction composition of different lupin seeds. Food Chem. 2008:107:830-844
  113. 113. Chew PG, Casey AJ, Johnson SK: Protein quality and physico-functionality of Australian sweet lupin (Lupinus angustifolius cv. Gungurru) protein concentrates prepared by isoelectric precipitation or ultrafiltration. Food Chem. 2003:83(4):575-583
  114. 114. Singla P, Sharma S, Singh S: Amino acid composition, protein fractions and electrophoretic analysis of seed storage proteins in Lupins. Ind J Agric Biochem. 2017:30(1):33
  115. 115. Carvajal-Larenas FE, Linnemann AR, Robert Nout MJ:Lupinus mutabilis: composition, uses, toxicology and debittering. Crit Rev Food Sci Nutr. 2016: 56(9):1454-1487
  116. 116. Lovati MA, Manzoni C, Castiglioni S, Parolari A, Magni C, Duranti M: Lupin seed γ-conglutin lowers blood glucose in hyperglycemic rats and increases glucose consumption of HepG2 cells. Br J Nutr. 2012:107:67-73
  117. 117. Sirtori CR, Lovati MR, Manzoni C, Castiglioni S, Duranti M, Magni C, Morandi S, D’Agostina A, Arnoldi A: Proteins of white lupin seed, a naturally isoflavone-poor legume, reduce cholesterolemia in rats and increase LDL receptor activity in HepG2 cells. J Nutr. 2004:134:18-23
  118. 118. Marchesi M, Parolini C, Diani E, Rigamonti E, Cornelli L, Arnoldi A, Sirtori CR, Chiesa G: Hypolipidaemic and anti-atherosclerotic effects of lupin proteins in a rabbit model. Br J Nutr. 2008:100:707-710
  119. 119. Viveros A. Centeno C. Arija I. Brenes A: Cholesterol-lowering effects of dietary Lupin (Lupinus albus var Multolupa) in chicken diets. Poult Sci. 2007:86:2631-2638
  120. 120. Weiße K, Brandsch C, Zernsdorf B, Nembongwe GSN, Hofmann K, Eder K, Stangl GI: Lupin protein compared to casein lowers the LDL cholesterol:HDL cholesterol-ratio of hypercholesterolemic adults. Eur J Nutr. 2010:49:65-71
  121. 121. Sirtori CR, Triolo M, Bosisio R, Bondioli A, Calabresi L, Vergori VD, Gomaraschi M, Mombell G, Pazzucconi F, Zacherl C, Arnoldi A: Hypocholesterolaemic effects of lupin protein and pea protein/fibre combinations in moderately hypercholesterolaemic individuals. Br J Nutr. 2012:107:1176-1183
  122. 122. Bähr M, Fechner A, Krämer J, Kiehntopf M, Jahreis G: Lupin protein positively affects plasma LDL cholesterol and LDL: HDL cholesterol ratio in hypercholesterolemic adults after four weeks of supplementation: A randomized, controlled crossover study. Nutr J. 2013:12:107
  123. 123. Bähr M, Fechner A, Krämer J, Kiehntopf M, Jahreis G: Consuming a mixed diet enriched with lupin protein beneficiallyaffects plasma lipids in hypercholesterolemic subjects: A randomized controlled trial. Clin Nutr. 2015:34:7-14
  124. 124. Hall RS, Thomas SJ, Johnson SK: Australian sweet lupin flour addition reduces the glycaemic index of a white bread breakfast without affecting palatability in healthy human volunteer. Asia Pac J Clin Nutr. 2005:14(1):91-97
  125. 125. Dove ER, Mori TA, Chew GT, Barden AE, Woodman RJ, Puddey IB, Sipsas S, Hodgson NM: Lupin and soya reduce glycaemia acutely in type 2 diabetes. Br J Nutr. 2011:106:1045-1051
  126. 126. Bertoglio JC, Calvo MA, Hancke JL, Burgos RA, Riva A, Morazzoni P, Ponzone C, Magni C, Duranti M: Hypoglycemic effect of lupin seed γ-conglutin in experimental animals and healthy human subjects. Fitoterapia. 2011:82(5):933-938
  127. 127. Schopen K, Ewald AC, Johannes BW, Bloch W, Rittweger J, Frings-Meuthen P: Short-term effects of lupin vs. whey supplementation on glucose and insulin responses to a standardized meal in a randomized cross-over trial. Front Physiol. 2017:8:198
  128. 128. Guillamón E, Pedrosa MM, Burbano C, Cuadrado C, Sanchez MC, Muzquiz M: The trypsin inhibitors present in seed of different grain legume species and cultivar. Food Chem. 2008:107(1):68-74
  129. 129. Pihlanto A. Mattila P. Mäkinen S. Pajari A.M: Bioactivities of alternative protein sources and their potential health benefits. Food Funct. 2017:8:3443-3458
  130. 130. Jayasena V, Chih HJ, Nasar-Abbas S: Functional properties of sweet lupin protein isolated and tested at various pH levels. Res J Agric Biol Sci. 2010:6:130-137
  131. 131. Kiosseoglou A, Doxastakis G, Alevisopoulos S, Kasapis S: Physical characterization of thermally induced networks of lupin protein isolates prepared by isoelectric precipitation and dialysis. Int J Food Sci Technol. 1999:34:253-263
  132. 132. Dahiya PK, Linnemann AR, Van Boekel MAJS, Khetarpaul N, Grewal RB, Nout MJR: Mung bean: Technological and nutritional potential. Crit Rev Food Sci Nutr. 2015:55:670-688
  133. 133. Mubarak AE: Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Food Chem. 2005:89:489-495
  134. 134. Yao Y, Hao L, Shi Z. Wang L, Cheng X, Wang S, Ren G: Mung bean decreases plasma cholesterol by up-regulation of CYP7A1. Plant Foods Hum Nutr. 2014:69(2):134-136
  135. 135. Yao Y, Zhu Y, Ren G: Mung bean protein increases plasma cholesterol by up-regulation of hepatic HMG-CoA reductase, and CYP7a1 in mRNA levels. J Food Nutr Res. 2014:2:770-775
  136. 136. Tachibana N, Wanezaki S, Nagata M, Motoyama T, Kohno M, Kitagawa S: Intake of mung bean protein isolate reduces plasma triglyceride level in rats. Funct Foods Health Dis. 2013:3(9):365-376
  137. 137. Maruyama N, Adachi M, Takahashi K, Yagasaki K, Kohno M, Takenaka Y, Okuda E, Nakagawa S, Mikami B, Utsumi S: Crystal structures of recombinant and native soybean beta-conglycinin beta homotrimers. Eur J Biochem. 2001:268:3595-3604
  138. 138. Itoh T, Garcia RN, Adachi M. Maruyama Y, Tecson-Mendoza EM, Mikami B, Utsumi S: Structure of 8Salpha globulin, the major seed storage protein of mung bean. Acta Crystallogr D Biol Crystallogr 2006:62:824-832
  139. 139. Kohno M, Motoyama T, Shigihara Y, Sakamoto M and Sugano H: Improvement of glucose metabolism via mung bean protein consumption: A clinical trial of glucodia tm isolated mung bean protein in Japan. Funct Foods Health Dis. 2017:7:115-134
  140. 140. Kohno M, Sugano H, Shigihara Y, Shiraishi Y, Motoyama T: Improvement of glucose and lipid metabolism via mung bean protein consumption: Clinical trials of GLUCODIA™ isolated mung bean protein in the USA and Canada. J Nutr Sci. 2018:7:e2
  141. 141. Anstee QM, Targher G, Day CP: Progression of NAFLD to diabetes mellitus, cardiovascular disease or cirrhosis. Nat Rev Gastro Hepatol 2013:10:330-344
  142. 142. Torre-Villalvazo I, Tovar AR, Ramos-Barragan VE, Cerbon-CervantesMA, Torres N: Soy protein ameliorates metabolic abnormalities in liver and adipose tissue of rats fed a high fat diet. J Nutr. 2008:138:462-468
  143. 143. Yang HY, Tzeng YH, Chai CY, Hsieh AT, Chen JR, Chang LS, Yang SS: Soy retards the progression of non-alcoholic steatohepatitis via improvement of insulin resistance and steatosis. Nutrition. 2011:27:943-948
  144. 144. Wanezaki S, Tachibana N, Nagata M, Saito S, Nagao K, Yanagita T, Kohno M: Soy beta-conglycinin improves obesity-induced metabolic abnormalities in a rat model of nonalcoholic fatty liver disease. Obes Res Clin Pract. 2015:9:168-174
  145. 145. Watanabe H, Inaba Y, Kimura K, Kaneko S, Asahara S-I, Kido Y, Matsumoto M, Kohno M, Tachibana N, Inoue H: Dietary mung bean protein reduces hepatic steatosis, fibrosis, and inflammation in male mice with diet-induced, non-alcoholic fatty liver disease. J Nutr. 2017:147:52-60
  146. 146. Xie J, Du M, Shen M, Wu T, Lin L: Physico-chemical properties, antioxidant activities and angiotensin-I converting enzyme inhibitory of protein hydrolysates from mung bean (Vigna radiate). Food Chem. 2019:270:243-250
  147. 147. Wongekalak L-o, Sakulsom P, Jirasripongpun K, Hongsprabhas P: Potential use of antioxidative mung bean protein hydrolysate as an anticancer asiatic acid carrier. Food Res Int. 2011:44:812-817
  148. 148. Li GH, Wan JZ, Le G-W, Shi Y-H: Novel angiotensin I-converting enzyme inhibitory peptides isolated from alcalase hydrolysate of mung bean protein. J Pept Sci. 2006:12:509-514
  149. 149. Bartholomae E, Incollingo A, Vizcaino M, Wharton C, Johnston CS: Mung bean protein supplement improves muscular strength in healthy, underactive vegetarian adults. Nutrients. 2019:11:2423
  150. 150. Yi-Shen Z. Shuai S. FitzGerald R: Mung bean proteins and peptides: Nutritional, functional and bioactive properties. Food Nutr Res. 2018:62. DOI:10.29219

Written By

Mitsutaka Kohno

Submitted: 10 May 2021 Reviewed: 17 August 2021 Published: 21 September 2021