Introductory Chapter: Dental Biofilms Associated with Caries

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Introduction
Caries is a localized, progressive, destructive process in which the mineral component of dental hard tissues dissolves. It is the result of multiple disturbances of the balance between the oral environment and the teeth. Due to this disturbed balance, microbial acid production occurs and the pH value decreases.
Dental caries is still the most common chronic infectious disease, especially in endangered and poor populations [1,2], but not so prevalent in the industrialized world and developing countries. Dental caries is a disease associated with biofilm. Plaque control is an important caries prevention strategy, because biofilm bacteria are the driving force of demineralization and caries development [3,4].
The course of dental caries development depends on several host factors, including location, morphology, composition, ultrastructure, and age of the tooth after eruption [5]. The very environmental conditions that exist at each site of the tooth explain the highly localized and complex nature of the caries process. The occlusal pits and cracks of the molars with their morphological characteristics create a retention area for the formation of biofilm and food retention, making them more prone to caries forming on the tooth surface in children.
The process of dental caries begins within the bacterial biofilm that covers the surface of the tooth. Numerous episodes of mineral loss and gain (demineralization and remineralization) occur on the enamel surface. If demineralization prevails over remineralization, the result will be a permanent and irreversible mineral loss, void formation, and continuous destruction of hard tissues [6,7]. Signs and symptoms of the disease range from the slightest underground loss of minerals to severe tooth destruction.
Clinical causes of caries include: 1. the presence of plaque containing acid-producing bacteria, 2. consuming carbohydrates that are easily fermented on a frequent basis (for example, sugar), 3. low saliva production or reduced saliva capacity to act as a buffer and. 4. genetic factors which make the host more susceptible to caries [8].

Components of dental plaques
The oral microbiome, the unique natural ecology of the mouth is an ecosystem or community of microorganisms that live in symbiosis with one another and with their host, which contribute positively to the individual's health. It is composed of many different bacteria, neutrophils leukocytes, macrophages, monocytes and lymphocytes. The largest portion of dental plaque is liquid and consists of water while 70% of the dry weight is made up of bacteria and the rest is polysaccharide and glycoprotein matrix adherent to the tooth surface [9].
Polysaccharide and glycoprotein matrix mainly consist of 10-20% waterinsoluble glucan, 1-2% fructan, approximately 40% bacterial and salivary proteins, variable quantities of lipid, calcium, phosphorus, magnesium and fluoride -the rest is water. The remaining microorganisms are later colonizers that join in the dental biofilm and with their metabolic activities have a competitive advantage in the community [10].
The dental bio-plaque formation is a complex structure, with a highly organized setting of functional symbiotic interactions between microorganisms [11].
The maintenance of health is closely dependent on the balance between the microorganisms that practice commensalism or mutualism between themselves and in relation to the host.
In addition to these interactions, under the influence of certain circumstances, internal or external, this homeostasis may be disturbed, resulting in changes in the microbial population and the induction of certain pathological conditions. An elevation or a depression in the relative presence of certain microorganisms often leads to disruption of homeostasis [12].
A common feature that can disrupt the microbial ecosystem is the import of nutrients, such as sugars. Carbohydrates induce the reproduction of microorganisms that ferment sugars, such as Streptococcus mutans and lactobacilli species. Through metabolizing these carbohydrates, the bacteria generate large amounts of lactic acid, resulting in the selection and dominance of acidogenic bacteria.
The acidogenic oral bacteria present in the dental biofilm, have the ability to generate acids, which are responsible for tooth structure demineralization. The biofilm, related to dental caries, functions in several ways. It is a result of bacterial proliferation and growth, acid/ base regulation at the tooth surface, and a calcium ion exchange between the tooth and the saliva [13].
Many studies have confirmed the role of Streptococcus mutans and some anaerobic bacteria like Fusobacterium and Actinobacilus as a primary colonizer in the initial attachment on the tooth surface, responsible for the formation of the premature biofilm community [14].
The composition of bacterial species like Streptococcus mutans and some anaerobes like Fusobacterium and Actinobacteria varies in the different areas in the mouth. In accordance with the condition and localization of biofilm in the oral cavity there are subclasses of biofilm, sub gingival and supra gingival dental biofilm. Supra gingival plaque usually comprises of aerobic bacteria, thus it is filled with oxygen. On the other hand, sub gingival plaque found in interdental spaces which are devoid of oxygen, consists of anaerobic bacteria.
Changing the conditions in the environment, pH levels, salivary flow, temperature, oxidation-reduction reactions, the growth and development of microorganisms also changes [15]. The normal pH value in the oral cavity varies from 6 to 7. Small deviations from these salivary pH values are a gateway for the multiplication and growth of pathogenic microorganisms, thereby changing the dental plaque formation. The various nutrients in saliva such as proteins and amino acids are an excellent breeding ground for bacterial growth [16]. Deviations from the normal temperature in the oral cavity ranging between 35 and 36 degrees also alter the chemical processes that affect bacterial metabolism. Such a persistent acidic environment within the biofilm results in the demineralization of tooth enamel [17]. However, it should be pointed that streptococcal mutans that produce glucans and biofilm are the primary and major etiological factors in the pathogenesis of dental caries [18].

The role of sucrose in biofilm cariogenicity
Numerous epidemiological and experimental studies confirm the causal relationship between sucrose and dental caries [19][20][21][22].
Sucrose causes an increased level of Streptococcus mutans and Lactobacilli and at the same time, a decrease in S. sanguinis levels thus leading to biochemical and physiological changes in the dental biofilm formation and enhancing their cariesinducing properties [23]. The cariogenic effect of sucrose is closely dependent on its concentration and duration of exposure [24,25].
Increasing the frequency of carbohydrates results in plaque accumulation whose persistence below critical pH values will cause demineralization of the enamel [26]. Compared to glucose and fructose, sucrose has the dominant cariogenic potential [27]. As a substrate for the production of extracellular glucan by Streptococcus mutans glycosyltransferases, sucrose takes precedence as a unique cariogenic carbohydrate [28].

Dental plaque, a trigger factor in the development of caries and periodontal disease
Scientific research cannot definitively define the concept of a basic, normal oral microbiome that could be associated with a healthy oral cavity for a number of reasons. First of all, because individual dental biofilms are unique, with varying susceptibility to disease. Then with the passage of time and the aging process, the microbial community changes as a result of the evolutionary processes, the ecological environment and the immune response of the individual. However, Grampositive microorganisms belonging to the streptococcus and actinomyces species are predominantly present in the oral microbiome with certain deviations.
When the transformation of the healthy microbiological community begins and caries occurs, changes in the oral microbiological environment happen, along with changes in the development of the disease [29].
The acidogenic properties of cariogenic microorganisms and the ability to form extracellular polysaccharides from sucrose maintain the acidity of the environment, thus reducing the diversity of microbial flora.
The acidogenic capacity of these microorganisms is the mechanism that is scientifically widely accepted in the process of demineralization of dentinal and enamel tooth structures, causing the development of carious lesions [30].
Dysbiosis in periodontal diseases is also associated with an increase in microbial diversity. The main pathogens in periodontal diseases consist of dominantly gram-negative microorganisms including Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia and Aggregatibacter actinomycetemcomitans [31].
The periodontal microbial community, as dominant in anaerobic conditions, is capable of disrupting the host's inflammatory response by causing nutritional imbalance and impaired availability of potential gingival fluid and blood substrates. The microbial communities in periodontal disease and caries are in symbiosis with the host. How this balance is maintained depends on the nutritional interdependence of the bacteria as well as the salivary glycoproteins which are a constant nutrient source of dental biofilms [29].

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The scientific advances in molecular biology have shed light on the bacterial communities present in the oral cavity, providing evidence that the prevalence of oral infections is due to impaired host homeostasis [32][33][34].

Dental indices
Dental indices are a numerical expression for assessing the prevalence and incidence of certain dental conditions, and for determining the need for treatment as well. They can be simple and cumulative. The simple indices determine only the presence or absence of a certain state, while the cumulative ones determine the current state, but also previous, past states. There are also so-called irreversible indices, which determine immutable conditions, such as caries. Reversible indices, in turn, note changing conditions, such as the amount of dental plaque accumulation.

Oral hygiene index (OHI)
Proposed and developed by John C. Greene and Jack R. Vermillion (1960), [35]. This index is composed of Debris and Calculus Index. Rules for scoring are: 1) Only permanent teeth are scored, 2) Third molars or incompletely erupted teeth are not scored, 3) The buccal and lingual debris scores are both taken on the tooth in a segment having the largest surface area covered by debris. 4) Interpretation: The minimum number of points for all segments in either debris or calculus score is 0. The maximum number of points for all segments in either debris or calculus score is 36. The higher the score, the poorer the oral hygiene.

Simplified oral hygiene index (OHI-S)
This index is a modification of the original OHI index, also developed by John C. Green and Jack R. Vermillion (1964), [35], in order to reduce the number of decisions made by researches, as well as to shorten the time of inspection. Unlike the original OHI index, rules for scoring are at least two of the six possible tooth surfaces which must be examined. The third molars are involved only as long as they are functional. Natural teeth with crown restorations and tooth surfaces reduced in height by caries or trauma are not recorded.

Plaque index (PI)
The PI as developed by Silness and Loe [36], assesses the thickness of plaque at the cervical margin of the tooth (closest to the gum). Four areas, distal, facial or buccal, mesial, and lingual, are examined. Each tooth surface should be well dried beforehand, and the examinations are performed with a dental mirror, an explorer and a well-light probe. The probe is passed around the cervical third of the tooth to detect the presence of dental plaque. A disclosing agent can also be used to visualize dental plaque. The results are expressed in four values. A zero indicates no plaque present; 1 indicates a film of plaque present on the tooth; 2 represents moderate accumulation of soft deposits in the gingival pocket or on the tooth that can be seen by the naked eye; 3 represents an abundance of soft matter within the pocket or on the tooth. Each area of each tooth is assigned a score from 0 to 3. Scores for each tooth are totaled and divided by the four surfaces scored. To determine a total PI for an individual, the scores for each tooth are totaled and divided by the number of teeth examined. Four ratings may then be assigned: 0 = excellent, 0.1-0.9 = good, 1.0-1.9 = fair, 2.0-3.0 = poor.

Gingival index (GI)
Also attributed to Loe and Silness [37], the GI assesses the severity of gingivitis based on color, consistency, and bleeding on probing. Each tooth is examined at the mesial, lingual, distal, and facial (or buccal) surface. A probe is used to press on the gingiva to determine its degree of firmness, and to run along the soft tissue wall adjacent to the entrance to the gingival sulcus. Four criteria are possible: 0, normal gingiva; 1, mild inflammation but no bleeding on probing; 2, moderate inflammation and bleeding on probing; 3, severe inflammation and ulceration, with a tendency for spontaneous bleeding. Each surface is given a score, then the scores are totaled and divided by four. That number is divided by the number of teeth examined to determine the GI. Ratings are 0 = excellent; 0.1-1.0 = good; 1.1-2.0 = fair; 2.1-3.0 = poor.

Conclusion
Dental biofilm, as a proven complex biostructure of microorganisms is the primary etiological factor for the development of the two most diseases in the oral cavity -caries and periodontal diseases. Despite the fact that it cannot be completely eradicated, it can be controlled. So, the risk assessment for its occurrence is essential for the preservation of oral health. An individual adaptive anti-plaque program should be applied to each patient with a recommendation for appropriate home oral hygiene, practicing daily tooth brushing with a fluoride tooth paste, interdental cleaning and a hygienic-diet regimen with reduced carbohydrate intake. By doing this, there is an optimistic possibility that these cost-effective preventive strategies may minimize the incidence of caries and periodontal diseases and their impact on specific systemic conditions. © 2021 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.