The Role of Micro Vortex in the Environmental and Biological Processes

This work presents a short review of the theoretical developments in the application of vortex dynamics to the processing of environmental and biological systems. The mechanisms of complex fluid-particle interaction in vortex dominated and non-vortex dominated flows are briefly discussed from theoretical and practical perspectives. Micro vortex propagation, characteristics and their various applications in environmental process engineering are briefly discussed. Several existing and potential applications of vortex dynamics in turbulent flows are highlighted and as well as the knowledge gaps in the current understanding of turbulence phenomenon with respect to its applications in the processing of solid-liquid suspension and biological systems.


Introduction
Hydrodynamic-mediated interactions often occur in many technical and natural environmental processes. In the case of turbulent flows, this leads to the formation of turbulent eddies of various scales and sizes [1,2]. These energy-carrying eddies often interact with particles and biological materials on various temporal and spatial scales. Eddy-particle interactions often play a crucial role in these processes and it is largely the dominant driver of mass and momentum transfer. In studying the dynamics of such complex interplay of forces, a good knowledge of the vortex dynamics and its influence on the fluid and particle dynamics is highly indispensable [3,4].
Turbulent mixing, particle dispersion, and bioreactions have been topics of intense and sustained interest in many scientific inquiries [5,6]. The role of mixing and turbulence-driven particle dispersion in many fluid-particle processes is wellunderstood owing an abundant body of knowledge from many scientific interrogations. However, turbulence as a phenomenon is still poorly understood due to its complex nature. Since mixing and chemical reactions are impacted by the presence of turbulence, it is therefore extremely important to understand the different scales of turbulence in mixing applications.
As mentioned earlier, turbulence has been shown to lead to the formation of eddies on different scales [7,8]. The spatial degree of mixing such as the macro, meso and micro mixing are governed by the different scales of turbulence [5]. Mixing especially at high Reynolds number is often characterized by irregular, The Role of Micro Vortex in the Environmental and Biological Processes DOI: http://dx.doi.org /10.5772/intechopen.93531 eddies, there exists many eddies of other scales smaller than the integral scale that continually transfer the kinetic energy of the fluid through the other length scales. The Batchelor and Taylor scales given in Eqs. (2) and (3)

Colloidal stability and interaction forces
Colloidal materials in environmental and biological systems consist of small particles with very large surface area. Their typical sizes are in the range of 0.001-10 μm as shown in Table 1. The stability of these colloidal materials when dispersed in fluid can be explained by their tendency to acquire electrostatic charges by adsorbing ions from their surroundings. Forces mediating particle-particle interactions can broadly be classified into the following categories depending on the chosen approach: contact forces due to the particle-particle collisions and non-contact forces due to molecular interactions at contact or interface. A brief description of these forces is hereby presented in the following sections.

Molecular dynamics approach
The molecular dynamics description of particle-particle interaction forces shown in Figure 3 is based on the molecular interactions through interfacial forces on the surface of the interacting particles [17]. Interfacial forces are generally assumed to act on a length scales smaller than the particle size and interactions are only possible when particles are in close proximity or during collisions. The interactions of particles in suspension depend on these surface forces which consists of the long-range ionic electrostatic repulsive forces and the short-range London-van der Waals attractive forces. Classical DLVO or colloid stability theory provides a quantitative description of the forces experienced by particles in close proximity by considering such interactions forces to be additive [18].
The magnitude of the London-van der Waals attractive force between two charged particles and the electrostatic repulsive force due to the electric double layer can be derived from their corresponding interaction potential energies [17]. Eqs. (4) Table 1.
Classification of particles in dispersion [16]. and (5) represent the magnitude of these forces while Eqs. (6) and (7) represent their respective potential energies. In addition to these surface forces, a number of other interfacial interactions such as the hydration effects, hydrophobic attraction, steric repulsion, and polymer bridging have been observed to mediate particle-particle interactions [19]. Additional forces due to the fluid-particle interactions must also be considered to fully resolve all the forces experienced by particles in suspension and this is briefly discussed in Section 3.
where R ij is the distance between two interacting particles (center-to-center), A H is the Hamaker constant, ε is the permittivity of the medium, ψ 0 is the surface potential of the particles, K is the reciprocal of the Debye length.

Micromechanical approach
In contrast to the molecular dynamics approach, the micromechanical description of particle-particle interaction relies on the geometric analysis of finite number of discrete sub-elements as shown in Figure 3. All particle-particle interactions within this context are described by contact forces in the normal and tangential directions, while considering the elastic force-displacement, inelastic deformations or plastic dislocations, solid friction, and viscous damping [17]. Depending on the simplicity of these interactions, a soft or hard sphere description can be given.
In the hard sphere model, only elastic force-displacement is allowed. Soft sphere model on the other hand allows for most of the interactions that are possible when two particles are in direct contact.
When all these contact forces are fully resolved, the behavior of the particles upon collisions or impact on a wall such as their translational and rotational velocities can be predicted with a high degree of accuracy. A detailed description of the micromechanical theory of particle collisions and its importance in the determination of particle trajectory in dispersed suspension is beyond the scope of this communication and is available elsewhere [20]. In addition to the contact forces, body forces such as gravity and buoyancy and surface forces due to the fluid are some of the other important forces acting on the particles and their quantification is highly indispensable in resolving the dynamics of particles in suspension [21]. Some of these additional forces are discussed in the next section.

Particle-vortex interactions in turbulence
In order to initiate meaningful interactions through the interfacial forces, particles must be brought in close proximity. This can be achieved through the mechanisms of Brownian motion, differential settling, or turbulent dispersion as shown in Figure 4. The probability of particle collisions and the frequency of such collisions also depend on the trajectory of the particle motion. Thomas et al. [22] identified two types of particle trajectories leading to particle collisions namely: curvilinear and rectilinear particle trajectories. The particles in suspension under the influence of turbulence will experience fluctuating fluid motion with the particle being transported by the fluid eddies that exists within the flow vortex [3].
Consequently, small particles suspended in fluid exist in an environment of small energy-dissipating eddies in most practical flow devices. Under such conditions, particle collisions are facilitated by eddy size similar to that of the colliding particles. Furthermore, studies have shown that in addition to the fluid properties,  the particle properties such as size, density, and porosity also play important role in particle-vortex interactions [1,23]. Smaller particles with lower density than the flow fluid have been shown to be fully entrained within the vortex in the case of a vortical flow produced by the interaction of two flow streams of unequal velocity. The particles will be in dynamic equilibrium with the carrier fluid and will faithfully follow the flow streamlines. On the other hand, large particles will be unaffected by the vortex due to their large inertia, while the intermediate particles will be driven from the center of the vortex to the periphery as shown in Figure 5. The determining factor in particle-vortex interactions is the ratio of the particle relaxation time to that of the fluid, which is given by the Stokes number (Eq. (8)). Depending on the flow scenario, the particles in suspension will experience additional forces such as the drag, lift, pressure gradient, virtual mass, basset, and viscous stress forces due to the fluid-particle interactions [4,24]. Taken together, all these forces will ultimately determine the trajectory, dynamics, and fate of particles in suspension. The trajectory equation is given in Eq. (9).
where is τ A the particle relaxation time, τ F is the time associated with fluid motion (fluid time), ϱ p is the particle density, d is the particle diameter, L is the length scale associated with the vortex while U is the flow velocity.

Particle dynamics and aggregate disruption
Turbulence is the main driver of particle interactions in many practical applications. Consequently, the particle dynamics in terms of the particle collisions, coalescence/aggregation, growth, and breakage is primarily controlled by the fluid turbulence. The aggregate stability under the influence of hydrodynamic force has been suggested to be a function of the binding or cohesive force F B and Vortex Dynamics Theories and Applications 8 the hydrodynamic breaking force F H . While the binding force is determined by the aggregate structure and physicochemical attributes discussed Section 2, flow turbulence is the principal factor in the case of the hydrodynamic force. Therefore, the dynamics of particle behavior depends on an interplay of collision-induced particle aggregation and cohesive force and the rate of aggregate breakage due to the hydrodynamic stress.
The aggregate cohesive strength τ is a function of the physicochemical and biological conditions as well as the aggregate properties, while the hydrodynamic stress σ depends on the design of the aggregation unit and the prevailing process conditions. Several empirical and theoretical formulations are available for predicting the aggregate cohesive force and the maximum hydrodynamic breaking force. The global hydrodynamic stress σ due to the shearing action of the fluid motion on the aggregate as well as the overall cohesive strength τ of the aggregate resisting the hydrodynamic loading assuming a uniform shape and constant porosity can be expressed mathematically in Eqs. (10) and (11) [25,26]. An equilibrium of particle dynamics is reached at the steady-state condition. In this state, a continued particle or micro flocs/aggregate attachment to the larger flocs/aggregate is prevented, or the breakup kinetics is equal to the turbulence-induced collision rates.
In assessing aggregate strength and resistance to hydrodynamic-induced breakup, two common approaches are normally followed namely: limiting growth and limiting strength. The former relies on the determination of the maximum floc size before rupture, while the latter is based on the micromechanical analysis of aggregate strength. Many empirical and theoretical formulations based on the mentioned approaches are available in literature (Eqs. (12) and (13)). Liu et al. [27] presented the yield stress approach for calculating maximum aggregate tensile strength τy at which breakage is likely to occur in the inertia range of turbulence (Eq. (12)). Similarly, Attia [28] presented a model for predicting the critical fluid velocity above which there will be aggregate disruption by estimating floc yield stress σy resulting from the dynamic pressure acting on the floc (Eq. (13)).

Case studies in environmental and biological systems
This section presents a short review of the recent studies on the applications of turbulence or hydrodynamics in the environmental and biological systems ( Table 2). Water purification, irrigation, water quality assessment, sludge dewatering, bioflocculation, and bio-clogging are some of the technical areas of application identified. It should be noted that improvements in the performance of the engineered processes (e.g., stirred tanks, shear reactors and photobioreactors etc.) in the identified areas of applications continue to shape the research focus in the field of environmental process engineering [29]. In this respect, studies have been conducted to determine how to accurately quantify the impact of hydrodynamic characteristics on the infectivity of bacteriophage MS2, a norovirus surrogate. Several studies also involved the development of bioreactors for testing the effect of hydrodynamic characteristics on microalgae and human enteric viruses [29][30][31][32][33]. The results obtained from the studies indicated that the hydrodynamic cavitation could trigger the inactivation waterborne viruses to levels defined in water quality directives. This was reportedly due to OH-radicals that form an AOP during the cavitation process and high shear forces inside the cavitation structure. Also, flow structures in a hydrodynamic filter have been numerically investigated [34]. In this study, tangential component of velocity was defined, and the three-dimensional pattern of the flow current/streamlines was obtained using their two-dimensional projection in the meridian cross-section of the filter, which allows one to discover the vortex structures. It was concluded that the optimal flow regime can be implemented by selecting the optimal correlation between the flow of liquid regime to be processed and the rotation frequency of the filtering baffle in the hydrodynamic filter. The remaining sub-sections describe how hydrodynamics, turbulence, and vortex dynamics are applied to achieve the desired process efficiency in other identified areas of applications-water purification, sludge dewatering, food processing, and self-purification of the water bodies.

Hydrodynamics in water purification process
In fluid engineering problems, research has consistently focused on identifying parameters that improve engineered processes including water purification and inactivation of pathogens [35]. While the conventional technique of disinfection by chlorination has been employed to kill pathogenic microorganisms in raw water, recent studies have shown that chlorine reacts with organic compounds in water and generates disinfection byproducts (DBPs), such as trihalomethanes (THMs), haloacetic acids (HAAs), etc. As a result, turbulence-induced inactivation has been studied as an alternative approach.
The effect of hydrodynamic parameters such as orifice size, orifice number and orifice layout of multi-orifice plate, cavitation number, cavitation time and orifice velocity on the microbial population have been investigated to determine how the desired process efficiency can be achieved [36]. Experimental results have shown that cavitation effects increased with decrease in orifice size and increase in orifice number, cavitation time and orifice velocity. Flow hydrodynamics and pipe material have also been shown to influence biofilm development in drinking water distribution systems (DWDS). Furthermore, biofilm development was inhibited at higher

Hydrodynamics in solid-liquid separation
In the design of process reactors, it is often necessary to tailor the separation technique to the dynamics and characteristics of the waste slurry that is being treated. Hence, several studies have been conducted to determine the influence of hydrodynamics on sludge processing. The optimum dosage values, which were obtained when flocculation performance was assessed based on surrogate indicators such as sludge volume index and supernatant turbidity, confirmed polymer bridging as the primary flocculation mechanism. Specific apparatus construction and reactor geometry were found to help maintain sludge suspension in a metastable state that is crucial for the formation of pellet-like compact agglomerates with better dewaterability properties [2,40]. Similarly, sludge disintegration, using rotor-stator type hydrodynamic cavitation reactor (HCR), has been experimentally investigated [41]. To determine the effects of cavitation (including thermal energy) and shear stress on sludge disintegration, the performance of the HCR with and without the dimples and temperature control was analyzed. The results indicated that when dimples were present and there was no temperature control, the reduction of sludge particles increased by 50-80%. Further, the disintegration performance increased with the rotational speed and was minimized at the highest inlet pressure. Several other studies leveraging on the hydrodynamics of the process reactor for fluid-particle separation are available in literature [42,43]. Many of these lab-scale studies have demonstrated the feasibility of turbulence-induced fluid-particle separation.

Hydrodynamics in food and paper processing
Hydrodynamic cavitation (HC) is a process in which high energy is released in a flowing liquid upon bubble implosion due to decrease and subsequent increase in local pressure. In food and beverage industries, hydrodynamic cavitators can be utilized for the purpose of extraction, emulsification, sterilization, disinfection, and homogenization [44]. HC, which can effectively induce sonochemistry by mechanical means, creates extraordinarily high of pressures of ~1000 bar, local hotspots with ~5000 K, and high oxidation (hydroxyl radicals) in room environment, without introducing new chemicals. For possible industrial application, the efficiency of HC has been studied by comparing the chemical oxygen demand (COD) removal efficiency of a Venturi device to that of an orifice plate. A sucrose solution and an effluent from a sucrose-based soft drink industry were treated. Results showed that the Venturi device recorded 90% COD removal efficiency after treatment period of three minutes. On the other hand, the orifice plate recorded 90% COD removal efficiency after 9 minutes [44][45][46]. Developing high-performance HCRs and revealing the corresponding disinfection mechanisms constitute the most crucial issues today [45].
Refining of cellulose pulp is a critical step in obtaining high quality paper characteristics, however, this process is slow and costly especially for refining longer conifer fibers which are the preferred source for high quality paper production and give the paper its strength. Recently, hydrodynamic cavitation was applied to the refinement conifer rich pulp samples [47].

Hydrodynamics in self-purification of water bodies
The self-purification ability of water bodies is related to the prevailing hydrodynamic conditions. Coupled hydrodynamic and water quality models have been used to investigate the spatial and temporal water quality variations of the water bodies. Using an Acoustic Doppler Velocimeter, the efficiency of aeration plug-flow device (APFD) in terms of water flow and dissolved oxygen (DO) have been determined experimentally [48][49][50]. Recent findings have shown that discharges from several rivers flowing into the New York/New Jersey (NY/NJ) harbor interact and interfere with one another. Such interactions can improve or inhibit water and contaminant flushing from the harbor. In Poyang Lake, three-dimensional velocity at various locations as well as the velocity distribution and turbulence characteristics were assessed, and plug-flow characteristics were analyzed. The two patterns of velocity and turbulence in horizontal sections observed are (1) near the aeration plug-flow device (APFD), the water flow was intensively pushed downstream and simultaneously recirculated; (2) farther away, the reflux area gradually decreased, and the velocity and turbulence distribution were more or less uniform. At the interfaces between two immiscible fluids -water and alkane of small carbon number, the amphiphilic PEO chain diffuses laterally, experiencing hydrodynamic drags from both phases. The absolute values of interfacial diffusion coefficients demonstrate a bigger contribution from the hydrodynamics from the water phase, which may be attributed to a stronger attraction between water and the PEO molecules [51].

Vortex patterns in fluid-particle reactors
The flow vortex patterns in many fluid-particle reactors are quite diverse and their analysis and characterization can provide an insight into the fluid and particle dynamics within the reactor. One important feature in many of these flow types is the presence of rotation or swirling or a combination of both resulting in anisotropic turbulence. A few of the dominant vortex patterns will be discussed in this section. It is also worth mentioning that the flow pattern is a function of the reactor geometry, stirrer or agitator, baffles, and the operating conditions. The focus will be on the common reactor geometries and flow regimes that are typically encountered in many practical applications.
A qualitative and quantitative analysis of the flow pattern in a processing reactor may be performed experimentally using an optical diagnostic imaging technique such as PIV or PDA or by numerical modeling. The selected approach in a given scenario will depend on the required detail of the flow field as well as the available resources. While the later provide a flexible option for the investigation of fluid flow problems, the former often complements or used in the validation of numerical model. A detailed description of specific techniques is beyond the scope of this submission. A few of the vortex patterns in some of these reactors are discussed below.

Rotatory flow vortex pattern (e.g., tubular reactor)
This type of flow pattern is commonly found in rotating tubular reactors with an enclosed flow induced by a stirrer. Previous studies have shown that this flow pattern has a profound influence on the performance of fluid-particle reactors, and this type of flow pattern can be found in many technical applications [29,39,52]. For instance, in terms of the stirrer-vessel configuration, the flocculation performance is significantly influenced by the impeller type and its speed. The axial impeller has been found to promote floc formation over a range of impeller speeds as it produces a more homogeneous distribution of local velocity gradients in comparison to the radial impeller. Also, high velocity gradients occur in the region around the impeller, which might significantly hamper the high rate activated sludge (HRAS) flocculation process [53,54].

Oscillatory flow vortex pattern (e.g., oscillatory reactor)
An oscillator is a device whose action causes intermittent velocity gradients over time, space, and direction, and can be used for colloid removal based on physical flow manipulation. An oscillatory flow pattern is commonly found in reactors with an enclosed flow induced by an oscillator. Results from previous studies have shown that gentle oscillation can promote simultaneous flocculation and particle agglomeration over a relatively short periods of time [42]. This technique, whose implementation can result in reductions of the reactor sizes and process times, has a strong potential to improve conventional separation processes. It has also been observed that higher oscillating frequency promotes faster upward vertical velocities, resulting in different sedimentation patterns and removal efficiencies generated by the different oscillation frequencies [43].   5.5.3 Swirling flow vortex pattern (e.g., stirred tank, hydrocyclone) A swirling type of flow pattern is commonly found in hydrocyclone [55][56][57] and counter-rotating reactors with an enclosed flow induced by a rotational swirling effect [58,59]. In addition to hydrocyclone, this type of flow field is also common in rotating reactors with baffles such as mixing tank, in which case, the flow is a combination of rotational and swirl dominated flow as shown in Figure 6c. In the case of a counter-rotating vortex reactor, it has also been found that the swirl ratio and micromixing time of the flow increases as the vortex reactor (MIVR) is scaled up, indicating a flow with stronger swirl yet less mixing efficiency [60]. In order to promote mixing and enhance the floc formation process, some baffles should be installed to break water flow co-rotation with the impeller [54]. In modifying the flow pattern to suit a particular condition, it is advisable to perform the optimization of the impeller shape for a particular vessel geometry [61,62].

Conclusions and future perspectives
This brief communication is a summary of the main concepts involved the fluid-particle research and technical applications. It is by no means an exhaustive contribution and readers interested in the details of the subject-matter are advised to consult other scientific information available on the subject-matter. The fluid process engineering is a field of active research and the there is an ever-increasing scope for the application of hydrodynamics, turbulence, and vortex dynamics. In addition to the identified areas of application, there are several emerging areas of application. As the turbulence research advance with better computing power and algorithms, it is hoped that there will be limitless scope for the application of vortex dynamics in fluid process engineering.