The Risk of Chromosomal Abnormalities in Cases of Minor and Major Fetal Anomalies in the Second Trimester

Currently, noninvasive intrauterine screening for most chromosome abnormalities is available, but ultrasound examinations also play an important role during pregnancy, by drawing the attention to the suspect of a possible abnormality. Fetal ultrasound disorders can be classified into two major groups: (1) Major abnormalities are actually diagnosed malformations that are often associated with certain chromosome abnormalities but may be associated with other disorders (multiplex malformation) and may occur as isolated disorders (e.g., cardiac disorders, duodenal atresia, omphalocele, cystic hygroma (CH)). (2) Minor anomalies (“soft markers”) are not abnormal in themselves but are mild abnormalities that may occur in normal pregnancy but also increase the risk of certain chromosome aberrations. The minor anomalies in the second trimester include thickened nuchal fold (NF), mild ventriculomegaly, pyelectasis, hyperechogenic bowels, hyperechogenic papillary muscle, and shorter long bones. Plexus choroid cyst which is classified as a minor marker does not increase the risk of Down syndrome but increases the risk of trisomy 18 (Edwards syndrome). We want to emphasize the importance of screening of minor and major ultrasound abnormalities in detecting chromosomal abnormalities in the second trimester.


Introduction
The chromosomal abnormalities account for a considerable part of the anomalies of the intrauterine fetus. Chromosomal abnormalities are the numerical (aneuploidies) and structural anomalies, which can be demonstrated by special methods of examination. The numerical and structural anomalies can be duplications/trisomies, deletions/monosomies, and other rearrangements on the chromosomes.
The autosomal trisomies and unbalanced rearrangements might cause severe multiple malformation syndromes and mental retardation. The rate of the intrauterine deaths is high. They cause mainly severe diseases; in some cases they are associated with anomalies which are incompatible with the postnatal life.
Besides the age, a history of aneuploidy, and noninvasive tests, positive ultrasound findings-which could be major structural abnormalities or minor ultrasound markers-can be an indicator for chromosome analysis [1].
In the second trimester, we examine the thickened nuchal fold (nuchal thickening, NF) in the horizontal section, level with the cerebellum and also containing the cavum septum pellucidum and the cisterna magna. This method, in contrast to the one used in the first trimester, measures the thickness of the soft tissue from the external aspect of the skin to the external aspect of the bone. Authors suggest 5 mm as a cutoff value [2,3]. A large nuchal translucency detected in the first trimester is a non-specific malformation, and in most cases it resolves during the second trimester of the pregnancy, although it might persist in certain cases to show a picture of nuchal thickening. It indicates an increased risk of trisomy 21 (Down syndrome) [4]. Benacerraf et al. were the first in drawing the attention to the fact that the nuchal fold or nuchal thickening (≥6 mm) observed during the ultrasound examination in the second trimester increases the risk of Down syndrome [4].
Numerous authors carried out similar examinations, subsequently demonstrating different results. Gray and Crane [3] tried to determine the proper cutoff values of nuchal thickening. They formulated two groups according to the size of the pregnancy, putting the values measured in weeks 14-18 to the first group and the data of weeks 19-24 to the second group. Considering the examinations, the suggested cutoff value in the first group (weeks 14-18) is ≥5 mm, and in the second group (weeks [19][20][21][22][23][24], it is ≥6 mm [5]. In their investigations, Gray and Crane [3] detected pathological karyotypes in 12 out of 47 cases (25.53%). Analyzing 23 cases, DeVore and Alfi found pathological karyotypes in 9 cases (39.13%) [6].
Other authors detected pathological karyotypes in 10% of second-trimester nuchal edema. Grandjean and Sarramon [7] processed the data of 3308 ultrasound examinations, with the help of 12 centers, and they found nuchal thickening in 38% of the cases of trisomy 21. According to the records of 12 centers, they performed karyotyping of 295 pregnancies in the second trimester with nuchal thickening. They found abnormal chromosomes in 22 cases (7.46%), 17 of these were trisomy 21 (5.76%) [7]. Gonen et al. [8] carried out karyotyping in 573 cases because of positive ultrasound findings during ultrasound examinations in the second trimester. There were 38 chromosome analyses carried out due to nuchal thickenings; in one case they found an abnormal karyotype (2.63%) [8]. Zimmer et al. discussed their cases of early second-trimester nuchal edema in 43 out of 1254 cases (3.43%); they detected pathological karyotypes, trisomy 21 being the chief cause in 27 cases, while other pathological karyotypes were blamed in 16 cases [9].

Cystic hygroma
Cystic hygroma is observable in the first and second trimesters. It can be differentiated from nuchal translucency and nuchal thickening by the clearly isolated fluid space and the typical medial line septation shown on ultrasound examination [11,12]. Cystic hygroma often persists and is often associated with aneuploidy (mainly monosomy X), other abnormalities, and fetal loss [12].
In the case of cystic hygroma, the risk of the chromosome abnormality is even higher than in cases of nuchal thickening. Bronshtein et al. found chromosome anomalies in 6 of 106 nonseptated cases (5.66%), whereas the risk of the chromosome abnormality in the 25  In terms of isolated cystic hygroma, fetal chromosome examinations were performed in 27 cases, and in 13 cases abnormal karyotypes were detected (48.15%). When cystic hygroma was associated with hydrops, a total of 13 cases was investigated, and 7 cases of chromosomal abnormalities (53.85%) were detected [10].
In the first and early second trimesters, Rosati and Guariglia [16] examined the volume of cystic hygromas by volumetric calculations. They also analyzed the proportion of associated chromosomal abnormalities, and in 8 of 14 septated cases, they detected pathological karyotypes (57.14%), whereas four pathological karyotypes (21.05%) were seen in the 19 nonseptated cases. In summary, the 12 patients with chromosomal abnormalities (36.36%) were divided as follows: trisomy 21 in 6 patients, monosomy X in 4 cases, and trisomy 18 and 13 in 1 case each [16]. Examining the septated and nonseptated cases together, Tanriverdi et al. found 13 pathological karyotypes (56.52%) among the 23 karyotyped patients [17]. Donnenfeld et al. applied karyotyping and fluorescent in situ hybridization (FISH) in the chromosomal investigation of the cells in the sample obtained from the fluid of the cystic hygroma and could justify monosomy X in 86% [18].

Nonimmune hydrops
Similarly to cystic hygroma, nonimmune hydrops can be observed both in the first and second trimesters and is often associated with aneuploidy (monosomy X or trisomy 21) and fetal loss [13].
Schwanitz et al. examined in separate groups the nonimmune hydrops cases associated with cystic hygroma and the individually occurring ones. When it was associated with cystic hygroma, abnormal karyotypes were demonstrated in 54.39%; without cystic hygroma this rate was 27.69% [24].
Has carried out investigations into nonimmune hydrops in the first trimester and found 9 pathological karyotypes (47.37%) out of 19 cases [25].
In the case of nonimmune hydrops, Beke et al. found chromosomal abnormalities in 4 out of 20 cases (20%), in each case monosomy X [10].

Cerebral and cranial anomalies
In the case of ventriculomegaly, a greater than normal amount of liquor expands the ventricular system. Cerebrospinal fluid may be overproduced, impaired, or obstructed. Increased pressure leads to thinning of the brain, creating hydrocephalus internus. Hydrocephalus externus is referred to as the proliferation of liquor in the subarachnoid space. Ultrasound first shows the expansion of the posterior horn of the side chamber. In cases of choroid plexus cyst and other cranial malformations, the risk of chromosomal abnormalities is also elevated.

Ventriculomegaly
In cases of cerebral anomalies, we define ventricular dilatation (ventriculomegaly) as a lateral ventricle with a diameter ≥ 10 mm. The ventricle of normal male fetuses is mildly wider (average 6.4 mm) than of the ventricle of normal female fetuses (average 5.8 mm) [26]. Ventriculomegaly increases the incidence rate of aneuploidy (trisomy 21 and other aneuploidies) [27,28].
Several authors were examining ventriculomegaly as one possible marker of the fetal chromosome abnormalities in the second trimester. Nicolaides et al. carried out karyotyping in 9 cases because of fetal ventriculomegaly, examining this among other anomalies, and they found chromosome abnormalities in 2 cases (22.22%) [20]. Following this, Mahony et al. [28] found 1 case of 20 ventriculomegalies (5%); Bromley et al. [27] observed 5 cases of chromosome anomalies examining 44 cases of ventriculomegaly (11.36%). The study of Gonen et al.
[8] also extended to more anomalies; as a part of it, they carried out karyotyping in 25 cases of ventriculomegalies and found no abnormal karyotypes. Terry et al. [29] detected abnormal chromosomes in 3 cases of the 25 examined fetuses (12%).

Choroid plexus cysts
According to the data of more authors, the choroid plexus cysts (CPC) mainly increase the risk for trisomy 18 [31,32]. According to certain studies, the risk level does not depend on whether the anomaly is unilateral or bilateral, but other studies show that the larger the anomaly (>10 mm), the higher the risk [33,34]. Certain authors suggest the 2-2.5 mm cutoff value [35], while others determined the 5 mm limiting value [33]. The cysts resolve practically every time, so the absence of the anomaly on a repeated ultrasound examination does not indicate a decrease in the risk [36].
Chudleigh et al. were the first who described cysts in the fetal choroid plexus [37]. Nicolaides et al. were the first who drew attention to the connection between the positive ultrasound finding of choroid plexus cysts and chromosome abnormalities, through a study including a small number of cases [20]. Thereafter, more authors demonstrated that fetal choroid plexus cysts mainly increase the risk of trisomy 18 and, to a lesser degree, the risk of trisomy 21.
Achiron et al. demonstrated 2 trisomy 18 cases from chromosome analysis of 30 CPC cases (6.67%); in 1 case the CPC was associated with other ultrasound anomalies [38]. Platt et al. found 4 cases of chromosome abnormalities from 62 cases (6.45%), in 3 cases it was trisomy 18, and in 1 case it was trisomy 21 [39]. Nadel et al. demonstrated 12 cases (5.13%) of abnormal chromosomes from a larger number of cases (234 cases), 11 of which were trisomy 18 (4.7%) and 1 case of triploidy, but from 234 cases there were 220 cases of isolated CPC, and in these cases they did not detect any chromosome abnormalities [40].
Chitty et al. [43] with a larger number of cases found chromosome abnormalities in 14 cases (2.13%). They examined separately the isolated cases and the cases associated with other ultrasound anomalies. In 603 cases, the choroid plexus cyst was not associated with other ultrasound abnormalities, and in 3 cases (0.5%), they found abnormal karyotypes (each was trisomy 18); in 55 cases the abnormality was associated with other ultrasound findings, and in 11 cases (20%), they detected abnormal karyotypes (in 9 cases trisomy 18, in one case trisomy 21, and in 1 case 47,XXX karyotype) [43]. With a similarly large number of cases, Ghidini et al. examined the incidence of chromosome abnormalities in cases of choroid plexus cysts, they only examined the isolated cases (765 cases), and they found abnormal karyotypes (trisomy 18) in 13 cases (1.7%) [44].
Coco et al. [45] in 2004 examined separately the isolated cases and those associated with other abnormalities, the cases associated with minor and major ultrasound anomalies. The rate of abnormal karyotypes was 0.55% in total. In isolated cases (311 cases) and in associated cases with minor anomalies (43 cases), they did not find any abnormal karyotypes. In the two detected cases of trisomy 18, the choroid plexus cyst was associated with major anomalies (16.67% from 12 cases) [45]. Sahinoglu et al. [46] performed karyotyping in 109 cases due to choroid plexus cyst, 3.67% of the patients had an abnormal karyotype, in 102 cases, it was isolated anomaly, and in 3 cases they found chromosome abnormalities (2.94%). In seven cases, the choroid plexus cysts were associated with other ultrasound anomalies, and they found chromosome abnormality in one case (14.29%); in all the detected four cases, the abnormal karyotype was trisomy 18.
According to a 2008 study, of the 435 cases identified by CPC, 390 undertook karyotyping. Of these, anomalies were found in 14 fetuses (3.59%), with the highest frequency being trisomy 18 (6 cases, 1.54%); in 1 case trisomy 21 (1, 0.26%) and 1 case trisomy 9 (0.26%) were detected. The incidence of monosomy X was 0.77% (3 cases). In one case, 47, XXY karyotype (0.26%) and in two cases other chromosomal abnormalities (0.51%) were confirmed (46,XX/46,XY and 46,XY/47,XXY/47,XYY mosaicism). Of the cases studied, other ultrasound abnormalities associated with the plexus cyst were found in 178 pregnancies, and 7 abnormal karyotypes were found (3.93%), 5 cases of trisomy 18, and 2 cases of monosomy X. Choroid plexus cyst occurred as an isolated anomaly in 212 pregnancies (it was not associated with any other fetal ultrasound findings (anomalies) and/ or poly/oligohydramnios), and 7 chromosome abnormalities (3.3%) were found, one of which was trisomy 18, 1 case trisomy 21, 1 case trisomy 9, 1 case monosomy X, and 1 case 47,XXY karyotype. The incidence of chromosome abnormalities was also similar in the case of unilateral and bilateral CPC; chromosome abnormalities were detected in the case of unilateral (3.3%) and in the case of bilateral anomalies (3.9%) [47].

Other cranial and cerebral anomalies
Other cranial and cerebral anomalies (holoprosencephaly, agenesis or dysgenesis of the corpus callosum, Dandy-Walker malformation, and cleft lip or palate) according to observations increase the risk of chromosome abnormalities, but they do not increase the risk of trisomy 21 [48]. A strawberry-shaped head mainly increases the risk of trisomy 18 [49].
Benacerraf et al. reported five cases already in 1984, where they found intrauterine cleft lip and palate and holoprosencephaly during the ultrasound examinations, and the chromosome analysis verified trisomy 13 in two cases (33.33%) [50].
Both Parant et al. [51] and later Tongsong et al. [52] reported 12 cases where they diagnosed intrauterine holoprosencephaly. The karyotyping in Parant et al. [51]'s study verified trisomy 13 in four cases (33.33%); in Tongsong et al. [52]'s study, there were three cases of trisomies found (25%); in two cases it was trisomy 13 (16.67%), and in one case it was trisomy 18 (8.33%). Bullen et al. reported in their study (2001) 68 cases of holoprosencephaly, they made karyotyping in 52 cases, where they found an abnormal karyotype in 38% (from 20 cases in 15 cases it was trisomy 13, in 2 cases trisomy 18, and in 3 cases other chromosome anomalies, from which in 2 cases they recognized the deletion of the long arm of chromosome 13) [53]. Studying the etiology of the holoprosencephaly, besides the maternal diabetes and trisomy 13, there are also monogenic types [54].
In cases of cleft lip and/or palate, Perrotin et al. [55] demonstrated in their study that in those cases they examined, there were no chromosome anomalies provided the abnormality was isolated (14 cases), and when they detected it together with other positive ultrasound findings, the incidence rate of the chromosome abnormalities was high. In 26 cases, there were 15 chromosome anomalies (57.69%), in 8 cases trisomy 13, and in 5 cases trisomy 18 [55].
Similarly, Beke et al. found in their study that the frequency of chromosome abnormalities was similar to other cranial abnormalities; with 44 fetuses being examined, they detected chromosome abnormalities in 7 cases (15.91%) [30].
The aim of Nazer Herrera et al. [57] was to estimate the prevalence of holoprosencephaly in relation to births. It turned out that this disorder occurred with low frequency in Chile, but it was associated with a high proportion of trisomy 13: occurring in 10.91% of the cases examined (6 out of 55 cases) [57].
In another study, this anatomical disorder and chromosome abnormalities were associated with a high proportion. According to a study in Japan, five out of seven holoprosencephaly cases (71.43%) showed the following differences: two children with trisomy 18, two children with trisomy 13, and one with 45,X karyotype [58].

Cardiac and thoracic abnormalities
The use of fetal echocardiography may further increase the importance of second-trimester ultrasound examinations for screening for chromosomal anomalies. This is particularly true in those cases where screening tests were not available in the first trimester or the pregnancy was only recognized later.

Echogenic intracardiac focus
In the fetal heart, the echogenic intracardiac focus is observable in 3-4% of normal pregnancies [59]; according to some studies, this rate might reach 10% in the Asian population [60]. Its incidence, by some authors, increases the risk of trisomy 21 [61][62][63][64][65] and, according to some studies, trisomy 13 [66]. According to some literature data, the risk is higher if the anomaly is observable in both ventricles [61].
Bromley et al. [61,62] examined in their studies (reported in 1995 and 1998) the correlation between the chromosome anomalies and the echogenic intracardiac focus detected during the ultrasound examinations. In 1995 they studied 66 cases and found trisomy 21 in 4 cases (6.06%) [61]. Chromosome abnormalities were found in 14 cases (4.83%) with a higher number of cases (290). In 1998, they examined 290 pregnancies in two groups and detected chromosome abnormalities (mainly trisomy 21) in 6.4% (125 cases) in that group where the maternal age was ≥35 years, and in the age group under 35 years (165 cases), this rate was 3.64% [62]. Winter et al. demonstrated in their similar report 16 cases of trisomy 21 of the studied 163 cases (9.28%) [65].
In a 2005 publication, the echogenic papillary muscle showed abnormal karyotype only when it was associated with other anomalies (4.69%), not in isolated cases [30].

Ventricular septal defect and atrioventricular septal defect
Many authors investigated the correlation among the chromosome abnormalities and the ventricular septal defect (VSD) and the atrioventricular septal defect (AVSD). In some cases, the authors managed the cases discovered in the second trimester or later in pregnancy (third trimester) and the cases diagnosed after birth as aggregated data. Hajdu et al. studied 21 cases of AVSD and found chromosome anomalies in 9 cases (42.86%); 7 of these were trisomy 21, 1 case trisomy 18, and 1 case trisomy 22 [67].
Tennstedt et al. [68] analyzed all those cases where the fetopathological examination verified cardiac malformation, and there were ultrasound examinations and karyotyping during the pregnancy (altogether 129 cases). A total of 36 fetuses had VSD, and 21 fetuses had AVSD. In cases of VSD, there were 15 fetuses (41.67%) with detected chromosome anomalies (8 cases of trisomy 21, 4 cases of trisomy 18, 1 case of trisomy 13, 2 cases of other abnormalities). If AVSD occurred, the incidence rate of the chromosome anomalies was 61.9% (from 13 cases, 9 trisomy 21 and 3 trisomy 18, and in 1 case other anomaly) [68]. Delisle et al. [69] studied the incidence of the chromosome abnormalities in cases of AVSD detected by ultrasound examinations during pregnancy, and from the 38 cases, they found 22 (57.89%) with chromosome anomalies (19 cases of trisomy 21, 1 case of trisomy 18, 1 case of trisomy 13, and 1 case of other positive finding). Paladini et al. [70] reported a study about the risk of chromosome abnormalities in cases of all cardiac (heart and large blood vessels) anomalies. In case of ventricular septal defect, the incidence rate of the chromosome anomalies was 45.33% (34 of 75 cases), and in case of atrioventricular septal defect, this rate was 80% (32 of 40 cases) [70]. From the 34 VSD cases, there were 14 cases of trisomy 21 (18.67%), 16 cases of trisomy 18 (21.33%), 2 cases of monosomy X (2.67%), and 2 other anomalies detectable. The distribution of the 32 abnormal karyotypes in case of AVSD was the following: 26 cases of trisomy 21 (65%), 3 cases of trisomy 18 (7.5%), and 3 other anomalies.
A 2006 study dealt with the VSD-related clinical characteristics of the Moroccan pediatric population, among others. The 44 patients involved in the research were between 2 and 3 years old. Six of them (13.64%) had Down syndrome. VSD patients who were later subjected to surgical correction were more likely to be able to avoid irreversible pulmonary artery hypertension [72].
According to another, Chinese publication, the association of trisomy 21 is also high in the case of complete AVSD (CAVSD). In a group of 35 patients with this type of anatomical deviation, Zhong et al. performed chromosome tests in 15 cases. A total of 13 chromosome abnormalities (86.67%) have been reported, of which 11 patients with Down syndrome have been identified [73].
Relatively few studies deal with long-term follow-up of Fallot tetralogy cases. According to Shuhaiber et al., the prospects can be positive for those who are being treated surgically in the case of the disease. In their study, however, they also refer to the related VSD and the high proportion of trisomy 21 (80.33%, according to their calculations) in their combined occurrence [74].

Other cardiac and large blood vessel abnormalities
In cases of other cardiac abnormalities and anomalies of the large blood vessels, the risk of chromosome abnormality is increased [75]. Paladini et al. [70] studied the incidence of the chromosome anomalies in cases of other cardiac and large blood vessel malformations. In the case of coarctation of the aorta, the incidence rate of abnormal chromosomes was 48.28%; in the case of double outlet right ventricle, it was 26.32%; in the case of hypoplastic left heart syndrome, it was 13.51%; in the case of truncus arteriosus, it was 33.33%; in the case of Fallot tetralogy, it was 45%; and in the case of atrial septal defect, there were no abnormal karyotypes found [70].
Other researchers also produced statistics for other heart disorders. Chromosome abnormalities were detected in six cases when investigating 33 fetuses [30].

Isolated hydrothorax
Only a few authors studied the isolated hydrothorax. Nicolaides carried out karyotyping in three cases because of isolated hydrothorax, in connection with an invasive intervention (blood gained by fetoscopy) performed due to other positive ultrasound findings, and found abnormal karyotype in one case [20]. Estoff et al. carried out karyotyping in 11 cases because of isolated hydrothorax, and in 1 case they found an abnormal karyotype (trisomy 21, 9.09%) [76]. In other studies, in two out of six (33.33%) cases, one case of trisomy 18 (16.67%) and one case of monosomy X (16.67%) associations were observed [30].

Abnormalities of the abdominal wall and abdomen
In the case of diaphragmatic hernia, abdominal organs appear in the chest due to a discontinuity in the compartment. About 90% of the lesion is on the left. The aperture of the compartment is not traceable; the abdominal organs of the fetus (liver, stomach, and intestines) appear in the chest, which dislocate the mediastinum and the heart. Chest space narrowing may lead to severe lung hypoplasia.
In cases of an omphalocele, organs (typically the intestines, stomach, and liver) protrude through the opening into the umbilical cord; the opening is in the center (median) of the abdominal wall, where the umbilical cord meets the abdomen.
Among the abdominal wall malformations, gastroschisis is a full-thickness defect of the abdominal wall, typically to the right of the umbilical cord, not including it. Through the open abdominal cavity, the small and large intestines protrude; there is no amnioperitoneal membrane covering the exposed organs.
During the ultrasound screening, the "double bubble" is a characteristic sign of duodenal atresia. The two "bubbles" are the distended stomach and dilated proximal duodenum.
Regarding anomalies of the abdominal wall and abdomen, the structural malformations associated with chromosome abnormalities are omphalocele and duodenal atresia. In cases with echogenic bowel-which is a marker and not necessarily abnormal in itself-the risk of trisomy 21 is higher [77][78][79][80]. The etiology of echogenic bowel could be other rare abnormalities (cystic fibrosis, atresia) in addition to the more frequent intrauterine infections [81].

Diaphragmatic hernia
Blancato et al. [82] demonstrated earlier (1992) by in situ hybridization the occurrence of tissue-specific mosaicism in some cases of diaphragmatic hernia. While during lymphocyte culturing there are no detectable anomalies (blood samples via cordocentesis or blood testing of the newborn), in cases of fibroblast samples (genetic amniocentesis and dermal biopsy), 12p isochromosome can be found. Donnenfeld et al. [83] carried out chromosome analysis in 15 cases of diaphragmatic hernia. In all cases they performed lymphocyte culturing (in 14 cases of fetal cordocentesis) in addition to the fibroblast tests. They found chromosome anomalies in seven cases (46.67%), of which there were three cases of trisomy 18, three cases of 12p mosaicism, and one case of other abnormality [83]. From the seven cases of anomalies, there were two with different results of the cells of different origins due to the mosaicism, and the 12p isochromosome was only detectable from the fibroblasts (amniocentesis and dermal biopsy) and not from the lymphocytes gained from the blood. In five cases, the examinations of the lymphocytes and the fibroblasts gave the same results (three cases of trisomy 18, one case of unbalanced translocation, and one case of 12p isochromosome). Huddy et al. studied 35 cases of diaphragmatic hernia, they examined the outcome of the pregnancy and the related abnormalities, and they found chromosome anomalies in 4 cases altogether (11.43%), of which 2 were trisomy 18 [84].

Omphalocele (exomphalos)
Many authors studied the abdominal malformations and the other anomalies associated with them. According to all data of the literature, the omphalocele (or exomphalos) is associated with chromosome abnormalities in high rates, mainly with trisomy 18, and in smaller proportion with trisomy 13.  [89] in 5 of 10 cases (50%), Rizzo et al. [90] in 7 of 12 cases (58.33%), and Fogel et al. [91] in 5 of 37 cases. In their recent study with a higher number of cases, Nicolaides et al. detected abnormal karyotypes in 42 of 116 omphalocele cases (36.21%); 32 cases of these were trisomy 18 (27.59%) and in 7 cases trisomy 13 (6.03%) [92]. Snijders et al. [93] found in higher rate abnormal karyotype, mainly trisomy 18, in 12 of 18 cases (66.67%). The following studies demonstrated almost the same results, Dillon and Renwick [94] in 12 of 43 cases (27.91%), Axt et al. [95] in 7 of 26 cases (26.92%), and Rankin et al. [96] in 30 of 98 cases (30.61%), while Stoll et al. [97] have found abnormal karyotypes in 17 cases out of 58 cases (29.31%). The majority of the abnormal karyotypes were trisomy 18. Axt et al. found the risk of chromosome abnormalities four times higher when the liver was placed intracorporeally than when in the extracorporal placement [95]. Barisic et al. [98] processed the data of 19 European centers and described 34 abnormal karyotypes in total for 137 omphalocele cases (24.82%). Most of the chromosomal abnormalities were trisomy 18 (21 cases, 15.33%); they described a lower rate of trisomy 13 (5 cases, 3.65%), one case of trisomy 21 and monosomy X, two cases of triploidy, and four cases of other chromosomal abnormalities. Salihu et al. [99] processed 29 cases, and 3 abnormal karyotypes were detected (10.34%); in all cases the liver was located intracorporeally.

Gastroschisis
Many investigators studied the gastroschisis cases in parallel with the omphalocele screenings, even though some of the studies were carried out with a smaller number of cases, so the results are not consistent. Rankin et al. found 1 case of chromosome abnormality in 133 gastroschisis cases (trisomy 13, 0.75%) [96]. Barisic et al. [98] also found abnormal karyotypes in 1.89% at a higher number of cases; they described chromosomal abnormalities in 2 out of the total 106 cases, of which 1 was trisomy 21 and the other was trisomy 13.
Other studies with a smaller number of cases did not indicate chromosome abnormalities; Mann et al. [85] studied 10 cases, Nicolaides et al. [20,92] 3 and 26 cases, Dillon et al. [94] 56 cases, and Axt et al. [95] 18 cases, and they could not detect any abnormal karyotype. Stoll et al. [97] studied 47 cases of gastroschisis, while Salihu et al. [99] described 15 cases; in these cases there were no abnormal karyotypes detected.
Gastroschisis is often associated with other anomalies. Along with polyhydramnios, special attention should be paid to the ultrasound examination of the fetus. This was observed by Ozawa et al. [100] in a study involving the analysis of 52 fetuses. A quarter of them had trisomy (13 cases, 25%), particularly high rates of Edwards syndrome detected in 10 cases, while Patau syndrome in 2 cases and trisomy 21 in 1 case [100].
In contrast, Stoll et al. [101] diagnosed only one fetus with chromosome abnormality in the case of gastroschisis (trisomy 21) representing 1.16% of the patients studied. They also stated that the etiology of omphalocele and gastroschisis is unclear and their pathogenesis is controversial [101].

Duodenal atresia (duodenal obstruction)
The ultrasonographic mark of the duodenal obstruction is the "double bubble." The disease is not always detected intrauterine; therefore some investigators involved the subsequently proven, postnatal cases to their study. Studies demonstrated that it mainly increases the risk of trisomy 21. Nicolaides et al. studied 23 cases and found chromosome abnormalities in 10 cases (43.48%) [92]. Bailey et al. detected 15 cases of chromosome anomalies of the 138 cases (trisomy 21) (10.87%) [102]. This rate at Heydanus et al. was 7 abnormal karyotypes out of 29 cases (24.14%), 6 of which were trisomy 21 [103]. Lawrence et al. found 11 cases of chromosome anomalies of also 29 cases (37.93%), in 8 cases trisomy 21 [104]. Beke et al. found abnormalities in 2 out of 17 studied cases (11.76%), including a trisomy 21 [30].

Echogenic bowel
It is controversial in international literature whether the karyotyping is justified in cases of echogenic bowel. Nyberg et al. was the first to draw the attention to the echogenic bowel increasing mainly the risk of trisomy 21 [105]. Subsequently Scioscia et al. reported 6 cases of chromosome abnormalities of 22 chromosome analyses associated with echogenic bowel (27.27%); in 5 cases trisomy 21 and in 1 case trisomy 18 were found [79]. Bromley et al. carried out 50 karyotyping associated with echogenic bowel and detected abnormal chromosomes in eight cases (16%), six cases of trisomy 21, one case of trisomy 13, and one case of monosomy X [77]. Sipes et al. studied seven cases and detected one case (14.3%) of trisomy 18 [81].
There were two greater studies fully investigating the expectable risk in association with echogenic bowel.
Slotnick and Abuhamad [106] separately screened the different measures of echogenity in their study published in Lancet, classifying them this way: grade1, mild increase of echogenity; grade2, moderate increase; and grade3, pronounced increase. They carried out altogether 145 karyotyping because of echogenic bowel and found abnormal karyotypes (all trisomy 21) in eight cases (5.5%). There were 6 cases of trisomy 21 of the 24 grade3 cases (25%), 2 positive cases of the 81 grade2 cases (2.47%), and no chromosome abnormalities of the 40 cases of grade1. They also studied the incidence of cystic fibrosis, they found 5 cases at grade3 and 2 cases at grade2, and they did not detect any cystic fibrosis at grade1 [106].
Strocker et al. [80] studied those cases separately, where the increasing bowel echogenity was associated with other malformations. They detected abnormal karyotypes in 15 cases of altogether 131 cases (11.45%). In 62 cases, the echogenic bowel was not associated with other positive ultrasonographic findings; in this group, there were 5 chromosome anomalies found, in 4 cases trisomy 21 (6.45%) and 47, XXY karyotype in 1 case. In the other group, there were 69 cases associated with other ultrasonographic findings; 10 of these cases carried chromosome abnormalities, 8 cases trisomy 21 (11.59), 1 case triploidy, and 1 case deletion X [80].
According to the observation of Beke et al., chromosome abnormalities were detected in only 3 out of 53 cases (5.66%), when the ultrasound image of the echogenic bowel was not isolated but associated with other defects [30].

Other abdominal malformations
Nicolaides et al. [92] also examined other abdominal malformations. In his study there were 24 cases of karyotyping due to lack of a visible stomach, and he found an abnormal karyotype in 18 cases (75%). In other 24 cases of chromosome analyses due to intestinal dilatation, there was 1 with abnormality (4.17%) [92].

Pyelectasis
Pyelectasis is the dilatation of only the renal pelvis and the calyces, in milder cases, while when the parenchyma is also affected by the longer lasting compression (becoming thinner), hydronephrosis develops. Obstructions of the urinary tract usually result in the dilatation of the proximal sections. Obstruction of the ureteropelvic junction is the main cause of hydronephrosis in the neonates.
According to some authors, an increase in the size of the renal pelvis elevates the risk of aneuploidies (mainly trisomy 21) [107][108][109][110]. We measure the renal pelvis in a horizontal section in the anteroposterior (AP) direction. We define renal pelvis dilatation (pyelectasis) as a pelvis with an AP dimension ≥5 mm. Hydronephrosis (a renal pelvis ≥10 mm) and enlarged, echogenic kidneys increase the risk of trisomy 13. Benacerraf et al. [107] studied 210 cases of fetuses with pyelectasis. They found trisomy 21 in 7 of the 210 cases (3.33%). The suggested criteria for pyelectasis were the following cutoff values, ≥4 mm until week 20 and ≥5 mm in weeks 21-30, and after week 30 the limit should be ≥7 mm [107]. Corteville et al. studied 127 cases and detected chromosome abnormalities in 7 cases (5.51%), 4 of these were trisomy 21 (3.15%) [109]. They suggested that until week 33 the cutoff value should be ≥4 mm; after week 33 this limit would be ≥7 mm. Under comparable conditions (121 cases, similar cutoff values), Wickstrom et al. found chromosome anomalies in three cases (2.48%), two of which were trisomy 21 (1.65%) [111].
Nicolaides et al. reported in their study 35 abnormal karyotypes that are found in cases of 258 fetuses with pyelectasis (13.57%) [92]. Their recommended cutoff value was ≥5 mm as a criteria for pyelectasis. Chudleigh et al. carried out chromosome analysis using 5 mm cutoff value in 737 cases of fetal pyelectasis. Studying the results of the karyotyping, they found abnormal chromosomes in 12 cases (1.63%), and 6 of these were trisomy 21 (0.81%) [108]. Besides less number of cases, other scientists did not verify the higher rate of chromosome anomalies. Gonen et al. found no abnormal karyotypes in 58 cases of fetal pyelectasis [8].
Beke et al. [30] processed 302 cases, of which 7 (2.32%) were found to have abnormalities. Of these, 1-1 cases of trisomy 21 and trisomy 18, two cases of monosomy X, and three cases of other chromosome abnormalities were found [30].
According to the findings of a 2006 study, contrary to previous opinions that male fetuses had a higher rate of chromosome abnormalities associated with pyelectasis, they refuted this. According to this new article by Bronstein et al. [112], there is no significant difference between the sexes in the karyotype associated with the anatomical disorder. When investigating 672 cases, 35 (5.21%) cases of abnormal karyotype occurred [112].
Based on Coco's and Jeanty [113]'s findings, pyelectasis alone does not justify an amniocentesis if it is not associated with anatomical and other abnormalities. However, 3.01% of their cases were associated with chromosome abnormalities as well [113].

Abnormalities of the extremities
Measuring the length of fetal femur and more recently the humerus is part of the biometric test. Following the limbs of the fetus, the joints, the hands, and the feet and their deviations can be brought into the field of vision. Limb disorders may appear independently or as part of syndromes, causing extensive ossification anomalies. Most disorders can be rarely recognized prenatally.

Short femur and humerus
On the basis of several studies of cases with shortened long bones (femur and humerus), the incidence rate for the abnormal karyotypes is elevated. According to the literature, a shortened humerus is a more sensitive indicator. Mainly the risk of trisomy 21 increases [114][115][116][117].
Studying the long bones, the main ambition of the investigators was to determine the proper method to estimate the measure of the retardation in growth, which increases the risk of chromosome abnormalities. The two main methods are fundamentally different. Some investigators calculate with the observed/expected ratio (O/E ratio) and determine the proper cutoff value by expressing the quotient in percentile (≤0.91) [8,115]. Other scientists calculate on the basis of the quotient of biparietal diameter (BPD) and femur length (FL) (BPD/FL) and consider abnormal the measure of >1.5 SD [114]. Calculating with both methods, most investigators found significant differences between the fetal groups of normal chromosomes and those with chromosome abnormalities. According to some authors, the difference was not significant [118,119].
Nyberg et al. [115] examined separately the parameters (O/E ratio) in the case of the humerus and femur and suggested the following cutoff values: humerus ≤0.89 and femur ≤0.91. If both the femur and the humerus are shortened according to the given cutoff values, the risk of trisomy 21 increased 11-fold [115].
Recent studies include both methods. In their report Snijders et al. draw the attention to the fact that although they found significant differences using both methods (O/E ratio, BPD/FL) between fetal groups of normal karyotype and trisomy 21, the screenings before week 18 of gestation are less suitable to predict trisomy 21 [116]. Vergani et al. [117] did not find a significant difference between measuring the O/E ratio and using the BPD/FL method; the difference reached the significance limit (p = 0.04). According to their report, the measurement of femur length is connected with the population, and each center should determine the cutoff values dependent on the population on their own [117].
According to a study, chromosome abnormalities occurred in 16% in cases of shortened tubular (long) bones, including 8% of trisomy 18 and 4-4% of monosomy X and trisomy 21 [30].
A Danish publication processed the data of 2718 fetuses detected with shortened femur between the gestational weeks of 17-22, of which 2.5% had chromosome abnormalities. In 11 cases, trisomy 21, in 3 cases trisomy 13, and in 8 cases trisomy 18 were found. Chromosome abnormalities were associated with a higher proportion of these anatomical disorders in the second trimester than the first trimester [120].

Conclusions
Ultrasound examinations play an important role during pregnancy, by drawing the attention to the suspect of a possible abnormality. The prenatal screening of minor ultrasound signs and major ultrasound anomalies and diagnostics is a very important part of the health service. The intrauterine screening and diagnostic methods, the ultrasound screening during the pregnancy, and the cytogenetic and molecular genetic examinations in the genetic centers made the early, intrauterine diagnosis of the chromosomal abnormalities possible.

Conflict of interest
The authors declare no conflict of interest.