Impact of Disturbances on the Biodiversity of Ijala-Ikeren Wetland Ecosystem in Niger Delta

An assessment of Ijala-Ikeren wetland in the Niger Delta of Nigeria was conducted for 9 months for biodiversity data comprising flora and specific aquatic fauna (amphibians and insects) in order to inventorise the species and ecosystem and to ascertain the effects of anthropogenic and natural disturbances on the wetland ecosystem. Five sites comprising three sites within the mangrove swamp and two ponds at the fringes of the mangrove ecosystem were sampled. Data collection methods include insect and amphibian sampling, and a few abiotic data. Empirical data on fish fauna was also obtained from fishermen. The pH value of the sampled sites in the mangrove ranged between 4.7 and 6.1, while temperature ranged from 28 to 29°C. Red mangrove species ( Rhizophora sp.) and few species of white mangrove ( Avicennia sp.) were dominant plants. Only three insect species from three families were encountered and thirteen species of amphibians from three families were only encountered in ponds at the fringes of the wetland. Amphibians had the highest value of species richness index (d) (2.75) and diversity index (H ′ ) (2.08). The acidic pH values maybe attributed to the influx of effluent from the Warri refinery about 2.5 km away and the low insect diversity is due to the salinity.


Introduction
Nigeria has the largest mangrove forest in Africa and the third largest contiguous mangrove forest in the world. The mangrove region is widest on the sides of the Niger Delta, 35-45 km and narrows towards the centre to a width of 15 km except for the channel of the Brass River, which has extensive mangroves far upstream [1].
The mangrove forests of Nigeria comprise principally only three families and six species as follows: Family, Rhizophoraceae (the red mangrove): Rhizophora racemosa, R. harrisonii and R. mangle. Of these R. racemosa is the most abundant covering about 90% of the mangrove forest. Avicenniaceae (white mangrove), Avicennia africana and Combretaceae (Laguncularia racemosa and Conocarpus erectus [1] and alike have been reported in most parts of the West African coast [2]. The Ijala-Ikeren Impact of Disturbances on the Biodiversity of Ijala-Ikeren Wetland Ecosystem in Niger Delta DOI: http://dx.doi.org /10.5772/intechopen.82604 sensitive to pollution and other environmental hazards, thus they reflect environmental changes and as a result are often used as indicators of the effects of human activity on water system and provide information on habitat and water quality [10]. Also some aquatic insects help keep the water clear by filtering it, others support in breaking down fallen dead leaves, while yet others clear the algae, allowing the water to produce more oxygen [11,12]. Amphibians have important roles within nature and their sensitivity to changing environmental conditions may help determine the health of an ecosystem. They constitute an important part of food chain and contain important pharmaceutical compounds on their skin [13]. The International Union of Conservation of Nature (IUCN) Red List of threatened species has identified amphibians as being the most threatened vertebrate group, with about 40% at risk of extinction. The extinction and decline have been variously attributed to habitat loss, pollution, climate change, wild fire and over-exploitation [14].
Recent studies have documented the incidence and severity of sediment contamination by heavy metals in mangrove ecosystems [15,16]. Contaminants are released via industrial activities and ultimately enter aquatic ecosystems [16,17].
Industrial effluent is a common anthropogenic impact on aquatic ecosystems including mangroves and this kind of pollution changes physical and chemical characteristics of aquatic systems, thus affecting the assemblage of aquatic fauna [17,18].
The mangrove forests and salt marshes control the tidal wetland ecosystem dynamics and contribute to the development and condition of the tidal saline wetland ecological communities [19]. Mangrove forests and salt marshes are highly valued ecosystems; however, mangrove forests and salt marshes maintain divergent ecological communities and a different suite of goods and services [20]. Climate change, environmental stress and anthropogenic activities can cause the conversion of mangrove forest to salt marshes, with positive and negative expected and unexpected consequences (e.g., effects upon fish and wildlife populations, ecosystem resilience, nutrient cycling, and variation of texture and soil geochemistry). The physicochemical conditions and ecological implications of these mangrove forests to salt marshes conversions are poorly understood, but would likely include changes in soil, water, associated wildlife populations and supply of some ecosystem goods and services [21,22].
The lack of publications on the biodiversity (particularly on the indicator species)-such as the insects and amphibians in Ijala-Ikeren wetlands of the Niger Delta, necessitated this study. Hence, this preliminary study was aimed at evaluating the abundance, community structure and diversity of biodiversity therein coupled with temperature, salinity and the pH levels of the ecosystem. We therefore, welcome future collaboration with available funding for a robust study.

Study area
The study was conducted in Ijala-Ikeren wetland located within latitude 05.55°N and 05.57°N and longitude 05.68°E and 05.70°E in Warri, Delta State, Nigeria (Figure 1). The wetland is a brackish network of creeks and creeklets, and marshes with one or more relatively narrow connections to the sea.
The study area which covers a space of 1 km 2 was divided into 2 regions: interior region bearing three sites; site 1, 2 and 3, and edges (fringes) region bearing sites 4 and 5. Altogether, 5 sites were engaged. Site 1 is located at the position, 05.55940°N, and 05.69630°E. The substratum here was covered with an admixture of black muddy and clayey sediment with an average depth of 0.2 m. Tidal flow was low and was at a low transparency. Vegetation here includes Rhizophora racemosa, Rhizophora harrisonii and Rhizophora mangle. This site is situated immediately after edges. Meanwhile, site 2 has the same substratum coverage and depth with site 1. The vegetation cover included thick stands of Rhizophora racemosa, Rhizophora harrisonii and Avicennia africana. The site is located at 05.44960°N and 05.69731°E. This site was very close to the oil pipelines. The last site in interior region; site 3, had the same substratum coverage and depth with sites 1 and 2. The vegetation cover were; R. racemosa, R. harrisonii and Nymphaea lotus (Water lily). The site is located at 05.55930°N and 05.69722°E. On the other hand, the two study sites representing the edges (fringes) region were sites 4 and 5. Site 4-the substratum was clayey with an average depth of 1.2 m, stagnant water with high infestation of invasive species such as water hyacinth (Eichhornia crassipes) most importantly. While at site 5-the substratum was clayey too with an average depth of <1 m. Stagnant water and some grasses occurred at this site. It was located at 05.55876°N and 05.69654°E. These sites (1)(2)(3)(4)(5) were selected on the strength of the tide and vegetation cover.

Environmental condition
Physicochemical parameters such as air temperature, water temperature, and pH were determined in situ. Temperatures were measured using the 0-100°C mercury in glass thermometer (Kurison Model-59). The pH was determined in situ using digital pH meter (consort 121, Belgium) adopted from [23]. While, salinity was determined in the laboratory by using HACHCO150 Model for total dissolved solid/ conductivity/salinity meter.

Vegetation cover
A description of the vegetation cover in and around the wetland was undertaken. Diversity of species of mangroves was recorded from the two studied regions. We used a [24] sampling technique for rapid assessment of the mangal vegetation of the brackish ecosystem in the study area by walking around at low tide. This involved a reconnaissance survey of the study stations of the Ijala-Ikeren mangrove ecosystem where a systematic sampling along the directed transects was applied to conduct the flora survey of the study sites.

Insect sampling
Insect sampling was conducted in the dry season (October 2013-February, 2014) and in the wet season (March-June, 2014). It was a 9 month study. Collection of samples was carried out through the use of sweep and kicks nets, and hand collection. Access to the mangrove interiors was through Falcorp Mangrove Park. Hand collections were made from wetland (mangrove) plants and from ant hive (Figure 2). The sieve-like dip net was used to collect aquatic insects from a distance on the mangroves while small aquatic nets were used to collect specimens in the aquatic plant (Nymphaea lotus and Water lily) while in water. Specimens collected were placed in vials containing 70% ethanol solution (C 2 H 6 O).
Kick sampling method modified after [23] was used to collect aquatic insect larvae (macro-invertebrates) for 3 min. We used 1 min to conduct searches before disturbing the water column and placing the net against the direction of the current for actual sampling. This method was also used to collect insects from water surface, from boulders, logs of wood and plants (Figure 3). All the collected specimens were identified using keys of [25][26][27]. The identified specimens were counted and placed into vials of 70% ethanol (C 2 H 6 O) which were well labelled indicating the location and date of collection.

Amphibian fauna
Sampling for amphibian was conducted using the Visual Acoustic Encounter Survey (VAES) method (i.e. listening to amphibians call and tracing the calls) at night and the anurans were captured by handpicking. The method was used along the trunk road (edges) traversing the area and in the interiors wetland (mangrove swamp) through the access to Falcorp Mangrove Park. Collection at the park was both during the day and at night.

Fish study
We used empirical method to ascertain the occurrences and diversity of fish species within Ijala-Ikeren wetland from the local fishermen at a few fish landings therein. A few fish species were equally sighted, observed and noted in-situ.

Statistical analysis of data
Overall diversity of soil insect was expressed as species richness, abundance, evenness and Simpson diversity index. Species richness and diversity patterns are a fundamental point for any scientific act in conservation biology [28]. Species richness represents the simplicity of describing communities at different scales and its broadly understood meaning. The complementary picture of overall diversity pattern of any community was documented by information about the abundance of species, the evenness of communities across the sampling region or the dominant species. Abundance is the kind of diversity measures that has inclusively been considered as equivalent to biodiversity per se [29] and referred to the sum of individuals in area. However, Simpson diversity index (D) is nearly the most tractable and statistically useful calculation [30]: Where D is Simpson diversity index, λ is an index of dominance. p i is the proportion of the community occupied by the ith species.
All parameters of diversity were calculated with PAST (version. 1.92) software running on Windows ® XP, [31].

Environmental condition
The mean values of physical parameters were recorded at the study sites. Air temperature ranged between 29 and 29.50°C in the mangroves (interior) and 30°C at the fringes (edges), while water temperature was between 28.50 and 29°C in the interior and 28-29°C at the edges ( Table 1). On the other hand, the measured pH values ranged between 4.8 and 4.9 in the interior and 5.3-6.1 at the edges, and salinity values were between 1.50 and 1.70 ppm ( Table 1).

Vegetation cover
The study also included the description of the vegetation in and around the wetland. It showed that the mangrove swamp in Ijala-Ikeren wetland is covered by three species of Rhizophora (red mangrove): Rhizophora racemosa, R. harrisonii and R. mangle. ( Table 2). Other flora species found were Avicennia africana (white mangrove), Eichhornia crassipes (water hyacinth) which occurred on the fringes of mangrove swamp floating in the pond and Pandanus candelabrum ( Table 2).

Insect composition and diversity
The sampled insects were identified into three orders: Hemiptera, Hymenoptera and Diptera. As shown in Table 3   Spatially, the three study sites in the interior (mangrove swamp) represented the highest percentage (75.5%) of species abundance, where Aquarius remigis and Formica sp. were the only identified species there. Meanwhile, the other abundance percentage (24.5%) was recorded at the edges (fringes) in the ponds (other two sites) in relating to the abundance of one species; Chironomus sp.
As shown in Table 3, the study sites at the mangrove swamp were the richest area with species number (2 species), site 1 had the greatest value in Margalef 's species richness index (d) and diversity of species out of these sites. On the other hand, site 2 had the least value of both indices.

Amphibian composition and diversity
A total of ninety-one (91) individual amphibians were collected in this study. Thirteen amphibian species belonging to three different families were identified: Bufonidae, Hyperoliidae and Ranidae. All these species were collected from the edges (fringes) of the mangrove swamp ( The 'E' which was very manifest in the unidentified Hyperolid (Hyperolius sp 1), Hyperolius occidentalis and Afrixalus dorsalis (Figure A1). These could be cryptic colourations to enable them escape detection by predators in their exposed habitats. Hyperoliidae was the most abundant one among the identified species (eight taxa) with an abundance of fifty-five individuals species. One feature of this group was the tendency to present different morphs (colour shade, pigmentation and design). Among this group, the dominant species was Afrixalus dorsalis (family: Hyperoliidae). The rare species were Hyperolius guttulatus and Ptychadena pumilio, Hyperolius sp. 2 and Afrixalus sp. About 30.8% of the identified species were grass frogs belonging to the family Ranidae, the genus Ptychadena had the dominant number of species (3 species); Ptychadena bibroni, P. Oxyrhynchus and P. Pumilio (Table 4). Meanwhile, only one species; Amietophrynus maculatus, was identified belonging to the family Bufonidae.

Fish fauna diversity
A checklist of the fish species is presented in Table 5. Catfish and Tilapia were the dominant species in the entire study area. A total of twenty representative species were recorded throughout.

Discussion
The study of vegetation in Ijala-Ikeren wetland clarified the richness of mangrove species, which is covered by about 95% Rhizophora species (red mangrove)  and Avicennia africana (white mangrove) with 5% other species, mostly Pandamus candelabrum and Eichhornia crassipes. This vegetation community richness of biotic community is more present and stable [32]. The complete absence of Ephemeroptera, Diptera, Odonata, Coleoptera, Trichoptera and Arachnida especially mesofauna were observed which may be due to the brackish water predominantly. A significant change was also detected in the environmental condition with particular reference to pH value, which was estimated at between 6.3 in 2009 and decreased to 4.8 on the average (acidic value). This may be attributed to the absence of the above listed arthropod orders especially as insects cannot tolerate the slightest change in salinity values beyond the fresh water concentration. This same scenario could be playing out for the amphibians, as none were recorded or encountered in the wetland (within the mangroves). This phenomenon has certainly explained the very low occurrence of species diversity of insects and amphibians populations and occurrences in the wetland ecosystems. This is particular for insects, as no aquatic insect larvae were encountered or collected during the survey period. Similarly, no amphibian eggs/tadpoles or adult forms were encountered inside the mangrove habitats of Ijala-Ikeren wetland. The acidity of water was explained by [6] who investigated the impact of refinery effluents around Warri Refinery and Petrochemical Company (WRPC) on surface waters of the creeks and soil qualities of host communities within and around Ijala-Ikeren wetland, and concluded the change in water pH to be 6.3, while the soil pH was 6.8 in Ijala-Ikeren wetland. This inference may be related to the discharge of these effluents from Warri Refinery and Petrochemical Company (WRPC) into Warri River which feeds the Ijala waters and also crude oil and its fraction seepages from numerous loading and off-loading jetties within the river which finds its way into the creeks in the Ijala-Ikeren wetland. They further stated that the Warri River is open to flooding which carries various contaminants. Contrary to our findings in water pH, the sediments studies conducted elsewhere in the Mexican pacific and Thailand, recorded alkaline pH values ranging from 6.91 to 7.83 [18,22].
Secondly, the discovery of illegal toxic waste dumpsites belonging to WRPC within these wetlands (pers. comm.) could increase the pollutant load of the study area. In turn, the highest concentration of calcium and salinity level was reported at the surface water within the wetland as reported by [6] though we did not analyse for calcium and heavy metals in this study. Also, heavy metals in varying concentrations and reasonable quantity from refinery effluents that resulted from Nigeria's crude oil could be one of the contributing factors to the acidic pH values. Especially, the metallic components in crude oil which are in the form of metalloporphyrin chelates, transition metal complexes, organometallic compounds, carbonyl acid salts of polar functional groups and colloidal minerals while other inorganic constituents of crude oil are sulphur, nitrogen, and oxygen [33].
Spatially, the study sites at the mangrove swamp was the richest habitat in terms of species number of insects, site 1 had the greatest value in Margalef 's species richness index (d) and diversity of species out of these sites. This result may relate to increasing the renewal of water during high tide as a common phenomenon in the area which could give rise to runoffs of debris from land into the mangrove. On the other hand, site 2 had the least value of both indices. As expected, this study site was characterised by its proximity to oil pipelines which over time had ruptured severely due to vandalisation and outlet that leads into the creeks.
Among the sampled insects, the presence of Chironomus sp. and Aquarius remigis at low salinity (<2 ppm) and acidic pH of surface water were detected. Chironomus sp. (Dipteran-Chironomidae) is tolerant of polluted water and an indicator of poor water quality with reduced oxygen concentration, and unhealthy ecosystem [34,35]. Therefore, this is a clear indication of the effect of the effluent on the biotic components of the water ecosystem.
Many amphibian species and their eggs are unable to tolerate and thrive in the brackish/salty environments of the mangrove (interior). One species, H. occipitalis was however an exception; this frog was encountered in several stagnant pools of water along the earth road leading to the wetland. Some individuals of this frog were sighted in a backhouse pond (edge) sharing boundary with the Ijala-Ikeren mangrove wetland.
So, the presence of Aquarius remigis and amphibian species; Hoplobatrachus occipitalis, at pH value of 4.1-4.9 indicates that the two species are indicator species of high acidic pH value. Those species whose presence occurred in acidic water bodies are tolerant of such environment and could be good candidate for monitoring changes in pH values in aquatic ecosystem especially brackish water including mangrove swamps. In accordance with the record of [36] these species of frog were found in some Nigerian waters at lower pH value which ranged from 4.1 to 4.2.
The presence of the insect (Chironomus sp.) and amphibians (Hyperolius concolor, H. burtoni, H. guttulatus and two unidentified Hyperolius species of Afrixalus dorsalis, A. fulvovitattus and Afrixalus sp.) are indicators of high levels of contamination of water from petroleum refinery effluents, however, we did not analyse the content of the effluent. They are indicators of acidic water of pH value between 5.3 and 6.1. In addition, the absence of aquatic insects in the adjacent mangrove swamps within Ijala-Ikeren wetlands tells us of the high level of contamination trends in the mangrove and creeks/creeklets in the area which may have generally affected the density of fish catch (outside overfishing, and using the wrong fishing gears) in the area which is a major source of livelihood for the host communities (personal communication). This invariably has affected the income of the people and also their protein intake. Despite the diversity of fish fauna (20 individual species), the fishermen remarked that the population of fish per catch is fast dwindling a situation they attributed to human induced factor rather than natural. [23] asserted that fish and crustaceans species are well supported in an alkaline pH value of water greater than 7. Furthermore, the absence of amphibians in the mangrove equally raises concern as amphibians have important roles to play within nature and their sensitivity to changing environmental conditions may help determine the health of an ecosystem.
Species richness measures the number of different kinds of species present in a particular area. Margalef 's Species Richness index (d) values range from 1 to 5 where the higher index indicates the greater diversity ( Table 3). Shannon diversity index (H′) takes into account the number of species and the evenness of the species. Diversity (H′) values less than 1.0 are considered very low; between 1.0 and 3.0 have moderate diversity, while any value greater than 3.0 signifies high diversity and ecosystem stability (i.e. similar to a pristine ecosystem). Evenness (E′) refers to how evenly each species is represented in a given ecosystem. Equitability assumes a value between 0 and 1 with 1 being complete evenness.

Conclusion/recommendation
Oil pollution from oil spills usually from illegal bunkering and pipeline vandalisation of the petroleum refinery facilities situated less than 2 km to the East of the wetland, and solid waste pollution are the major threats to this unique wetland. The Ijala-Ikeren wetland ecosystem has been affected by industrial activities and pollution, and these have affected the insect species and the amphibians. Based on the findings of this study, it has been established that the wetland ecosystem is highly stressed from contaminants with records of high acidic pH values which has resulted in the absence of aquatic insects and reduced fishes. The study pointed out that over 5 years (between 2009 and 2014) drastic changes in pH values occurred in the wetlands, as it reduced from 6.3 in 2009 to 4.8 in 2014. This could deteriorate more to a very precarious situation in the next 5 years if the pollution source is still left unattended to.
The study has shown that amphibian population in Ijala-Ikeren wetland appeared not to be under threat or extinction in spite of their absence at the mangroves; as thirteen (13) species were encountered with some equally having preference for acidic ponds. The results also showed that degradation of the wetland is on-going and it will be recommended that wetland conservation should be encouraged to forestall further loss of biodiversity. In doing this, adequate channels to set up a national awareness campaign among coastal and marine communities in the Niger Delta in conserving wetlands be identified. This becomes necessary as there are over nine (9) enacted national legislation/Acts for the protection of wetlands but their enforcement has been very weak. So government needs to mobilise in order to save our wetlands.
Government and non-governmental agencies should encourage studies on the inventory of aquatic insect species of water bodies in the host communities around the refinery to determine the health and pollution status of the aquatic ecosystem via funding.