New Results in the Theory and Practical Application of Color

The chapter provides new data on color phenomenon that we have discovered in recent years for complex and simple substances. For example, these are the effects of the relationship of physical and chemical properties and color characteristics (in color systems RGB or XYZ) of compounds (color properties principle). In particu-lar, the effects of conjugation of ionization potential and electron affinity of light-absorbing molecules in the visible region were found. The theoretical explanation of these effects from the position of quantum theory is given. Using mathematical statistics, it is shown that the effects can be used for practical measurement of various properties of complex multicomponent substances. The results indicate the practical use of these effects in chemical technology, nanophysics, and medicine.


Introduction
Even the Arab alchemists, the first chemists of the seventeenth and eighteenth centuries, talked about the relationship between color change and changes in the physical properties of various substances [1]. For example, the temperature of substances and their chemical transformations were estimated approximately by color. Here, new data on color phenomenon that we have discovered in recent years for complex and simple substances is provided. For example, these are the effects of the relationship of physical and chemical properties and color characteristics of compounds ("color properties principle") [2][3][4][5][6][7][8][9][10]. Color characteristics were measured by standard methods in colorimetric systems RGB or XYZ [11][12][13]. In particular, the effects of the relationship between the vertical ionization potentials and the electron affinity of light-absorbing molecules in the visible region were found [14][15][16][17][18]. The results indicate the practical use of these effects in chemical technology and nanophysics. We assume that the cause of these phenomena is quantum entanglement and strong correlation of electron states [19]. We established new physical effects between spectral densities (integral absorption, reflection, and transmission characteristics) with ionization potential and electron affinity [2-4, 15, 18-21]. We propose to use these effects in determining the energies of electronic states. Methods for determination of IP and EA for molecules and organic semiconductors have been developed. We propose to use these effects in determining the energies of electronic states. In addition, the color characteristics of biological fluids were investigated. In addition, we have determined the averaged color characteristics of the electromagnetic spectrum for aqueous solutions of hemolyzed blood, plasma, and serum from 100 donors and 95 patients with different diagnoses and different severities of their conditions. From the averaged absorption spectra, we calculated the color characteristics of the hemolyzed blood, plasma, and serum from the donors and patients by the standard CIE procedure. The blood is considered as a single, indivisible light-absorbing system in studying complex biological specimens. We studied the relationship between the color characteristics of human blood in normal and pathological conditions [22][23][24][25][26]. Let us consider these aspects in more detail.

Estimation of physicochemical properties of complex systems according to color characteristics
We have discovered new optical effects of the relationship between the physicochemical properties and color characteristics for very complex chemical systems [2][3][4]. In particular, the dependencies between the properties and color characteristics of multicomponent hydrocarbon systems are investigated. Dependencies between color coordinates (luminosity) and various physical and chemical characteristics of these substances are established. All results are confirmed by statistical data processing. The dependence of the properties on the CCs is linear (the law "color-properties"): where Z is one of the physical or chemical properties, q is the one of the color characteristics of the substance (e.g., color coordinates X j , Y j , Z j in the XYZ system or R j , G j , B j in the RGB system; or chromaticity coordinates x j , y j , z j in the XYZ system or trichromatic coordinates r j , g j , b j in the RGB system; j, standard light source A, B, C, or D), and B 0 , B 1 are the empirical constants dependent on the type of the source and the class of researched substances and dimensional properties.
Color coordinates of (X, Y, Z), coordinates of chromaticity (x, y, z), hue (λ), and luminosity (L) have been taken as color characteristics [11][12][13] . CCs of multicomponent hydrocarbonic systems have been determined by the technique of the International Committee on Illumination (Commission Internationale de l'Eclairage, CIE) [11] for four standard sources (illuminants) A, B, C, and D 65 . The technique, corrected for optically transparent [13] medium, has been used. Electron absorption spectra of multicomponent hydrocarbon systems have been determined in toluene solutions in the range of 380-780 nm with the use of automatic spectrophotometer.
The CCs were defined on the methods CIE [12,13] in the revised version to optical transparent solutions via the transparent coefficients-τ(λ). The color properties were calculated from formulas (2)-(7): where X, Y, and Z are the tristimulus values for system CIE; Е(λ) is the spectral power distribution for the spectrum of emission source; x, y, and z are three colormatching functions CIEXYZ; and τ(λ) is the spectral function of transparent coefficients in the visible region.
The transparent coefficient is defined on known relationships: where D is the optical density of solutions, c is the concentration, and L is the thickness of absorption layer.
For more hydrocarbon systems, the solutions were prepared with concentration near 0.002 g/l; similarly, as in this case, we got the result with minimal errors.
The relations for X, Y, Z are presented in matrix form [12]: where Ф XYZ is the column vector of color coordinates for objects of investigation in the system XYZ, Е XYZ is the product matrix of spectral power distribution for standard source and three color-matching functions, and Т is the column vector for coefficient of transparency.
The chromaticity coordinates were calculated on formulas in the system CIE (12). x where x, y, and z are chromaticity coordinates.
In Table 1 the defined CCs of multicomponent petrochemical systems are given [3,4]. As it can be seen from the results of the calculations, CCs at the identical radiation source are close among themselves despite their different nature. Obviously, the reason of similarity of color properties is the similarity of the absorption spectra of the systems researched. Also the research has shown that multicomponent petrochemical systems do not have color isomerism, i.e., their CCs change depending on the radiation sources.
The coefficients B 0 and B 1 Eq. (1) have been calculated by the method of least squares. As the criterion of adequacy, the correlation coefficient R and the meansquare deviation have been taken. Some results of the calculations are given in Table 2. The received results show that for all the researched petrochemical systems, there is correlation dependence PCP from CCs [2][3][4].
In many processes, it is necessary to take express control of the PCPs. Therefore, the dynamic form of Eq. (8) has been investigated in the author's very last investigation of more than 300 of multicomponent hydrocarbon systems: where ΔZ is the change of the physicochemical property and Δq is the change of CCs. Eq. (8) means that a change of properties is proportional to the change of color for any colored substances.
The received results show that for all the researched petrochemical systems, there is correlation dependence PCP from CCs. The correlation coefficient R and the standard deviation were used as the criterion of adequacy. Some results of calculations are given in Table 2. Properties such as relative density (ρ); number-average molecular weight (M in Dalton); Conradson carbon residue (g in weight.%); activation energy for viscous flow (Ea in kJ/mol). The results show that for all studied petrochemical systems, there is a clear dependence of PCP on CCs [2][3][4]. These correlations allow the determination of PCP substances using CCs. Such dependencies are necessary for quality control of oil distillates and oil products. In addition, there is an opportunity for remote control methods of environmental pollution by oil and oil products. For example, it is possible to determine in a few minutes such properties of formation oils as molecular mass, viscosity, density, the index of thermal stability, the index of reactivity of fractions in coking, thermal cracking processes, etc.

Introduction of electronic phenomenological spectroscopy
The method of electronic phenomenological spectroscopy (EPS) was first proposed by Mikhail Dolomatov [2,3]. In recent years, this science direction has been intensively developed by the Dolomatov group at the Oil Technical State University and Bashkir State University (Ufa) in Russia. There are the following approaches and physical phenomena in the basis of EPS: Unlike conventional spectroscopic methods, the EPS studies substances as a comprehensive quantum quasicontinuum without separating the spectrum of the substance into characteristic spectral bands by certain resonance frequencies or wavelengths of individual functional groups or components. The spectrum is  studied as a single system (broadband signal) from a set of electronic states. Therefore, at this system integral level, there are new physical effects, not previously known. For example, the effects of the relationship of integral optical characteristics with different macroscopic and quantum properties of the substance as a whole by quantum quasicontinuum "spectrum-properties" and "color-properties" are observed. Qualitatively new physical phenomena appear when considering systems interacting with radiation in a wide optical spectrum. According to these laws, changes in the physical and chemical properties of substances cause a change in the integral characteristics of absorbed, reflected, or emitted radiation in the ultraviolet (UV), visible, and near-infrared (IR) regions of the electromagnetic spectrum. This allows the use of EPS methods for the study of individual and complex multicomponent substances. For example, there may be a relationship between the integral force of the oscillator and some physical and chemical properties Z: Here θ is the integral absorption-integral oscillator force (IOF), which has a simple physical explanation, namely, the area under the radiation absorption curve for the visible and UV regions of the spectrum, l nm mole À1 cm À1 ; c is the constant depending on the method of measuring the spectrum, the nature of the substance, and individual for each property.
Therefore, it can be assumed that there is a relationship between any integral optical characteristic of a wide-spectrum signal ( Figure 4) and properties having the form.
Here, P is the integral spectral parameter, for example, integrated oscillator power, color characteristics, integral autocorrelation function, or relative imperial parameter and others into Figure 1. Obviously, a special case of Eq. (10) is the effect of "color-properties" (1) (we found with the coauthors O. Kydyrgychova, L. Dolomatova and V. Kartasheva in 1999 [5]). Phenomenological spectroscopy methods have been developed for identification and simultaneous determination of a set of different physical and chemical properties of natural and technical multicomponent organic systems, as well as properties of individual substances. For example, in a few minutes, it is possible to determine such properties of formation oils as the average molecular weight, viscosity, density, thermal stability index, index of reactivity of fractions in the processes of coking and thermal cracking, etc. EPS methods were adopted in the oil and petrochemical industry [2][3][4], environmental monitoring [3], biophysics and medicine [24,25], nanotechnology and molecular electronics [15][16][17][18], and space exploration.
For science and technology, of interest are laws of the relationship of the integral characteristics of the spectrum and the electronic properties of matter. The knowledge of the electron structure of the molecular substances and materials has the fundamental importance for solving real problems in many fields of science and technology (physic of solid state, chemistry, electronics, electrical engineering). Despite progress in the experimental and quantum methods in some cases, there are significant discrepancies between the predicted values and experimental results of electron structure determination of complex materials and compounds. Many compounds and some materials for nanotechnology are characterized by complex structure and chemical and phase instabilities. Therefore, it is necessary to create new methods for assessing electronic structures, for example, ionization potentials, affinities to electron, and some other properties.
Hence the difficulty of determining the first ionization potentials (IP), the affinity of electrons (EA) and other characteristics of the energies of electronic states for such systems.
As known, ionization energy is the energy required to remove an electron from an atom or molecule. The unit of measurement of this physical quantity is the amount of energy required to remove one electron from one atom or molecule, expressed in electronic volts. The ionization potential (IP) is the electrical potential at which an electron leaves an atom or molecule, overcoming the forces of attraction. This process forms a positive ion [27,28].
If during the electronic transition the geometry of the molecule changes minimally, it is said about the vertical IP. Next, we will consider the vertical potential only. According to the theorem of Koopmans, the first vertical ionization energy of a molecular system is equal to the negative of the orbital energy of the highest occupied molecular orbital (HOMO).
The electron affinity (EA) of an atom or molecule is defined as the amount of energy released or spent when an electron is added to a neutral atom or molecule with the formation of a negative ion [28].
In the chemistry IP and EA are the characteristics for ability of molecules to donor-acceptor properties [27]. These physical values may be used for the determination of the indexes of reactivity of molecules (a characteristic of its chemical activity).
In previous works [2,14,20,21], we established new physical effects between spectral densities (integral absorption, reflection, and transmission characteristics) with IP and EA. We propose to use these effects in determining the energies of electronic states. Methods for determination of IP and EA for molecules and organic semiconductors have been developed. We propose to use these effects in determining the energies of electronic states.
The IP and the EA of materials were estimated from the empirical dependencies linking these characteristics with the integral parameter of UV and/or vis spectrum: where Е is effective ionization potential or effective electron affinity, eV; α1 and α2 are empirically determined coefficients, and P is the integral spectral parameter. For example, integrated oscillator force (IOF), color characteristics, integral autocorrelation function or relative empirical parameter, and others ( Figure 1).
The first experiments in the detection of the phenomenon (2) were carried out in 1988-1992 together with the Dr. G. Mukaeva [14]. The dependence of IP and EA on the integral oscillator force (IOF) was established by the results of the study of about 200 optical spectra of atoms and organic molecules: Integral spectral characteristic can be any physical value of general absorption or emission of electromagnetic radiation, such as integral oscillator force (IOF): where θ is a reflection of quantum continuum as the sum of different states of electron, for example, all vibration states and electron states of transition among different levels, f(ξ) is spectral function of absorption or emission of radiation, and δ is a range of resonance wavelengths or frequencies.
Let us consider the method, which was proved in our previous works [14,15]. The IP and EA are estimated according to empirical dependencies which link these characteristics with logarithmic integral index of absorption (1).
Here Е is the ionization potential or an electron affinity, eV; α 1 and α 2 are empirically determined coefficients, eV and eV Ánm À1 , respectively.
where ε(λ) is the molar extinction coefficient, l mol À1 cm À1 ; θ 1g is the integral logarithmic index of absorption (logarithmic IOS), Ánm; λ 1 and λ 2 are borders of the spectrum in UV and (or) visible region, nm; and λ 1 and λ 2 are the borders of wavelength of the spectrum in UV and (or) visible region. Table 3 shows the corresponding coefficients for the dependencies (16) in different classes of organic molecules.
Breakthrough research in this area was done in collaboration with Dr. D. Shulyakovskii, Dr. E. Kovaleva, Dr. G. Yarmuhamedova, N. Paimurzina, and K. Latypov [20,21]. We established the following regularities, which connected the integral parameters of the spectrum with IP and EA (18)- (21).
In the calculation of integral parameters using the autocorrelation function of the signal, we have used the techniques adopted in statistical physics and spectroscopy [29]. We presented the energy spectrum of the molecule in the form of the integral of the autocorrelation function (IACF), frequency-dependent transitions. The integral autocorrelation function (ACF) is defined by the following formula:  Table 3.
Coefficients of dependence (16) for homologous series.
In [20] we proposed numerical parameter from IACP in the optical spectra was determined with the logarithmic function. The parameters of the ACF are because numbers are calculated using definite integral.
where the numerator of fraction is the integral autocorrelation function (IACF) in the UV spectral region; the denominator is IACF in the UV-vis spectral region; ν, ν þ Δν; ν 1 , ν 2 , and ν n are the spectrum boundary 10 14 Hz; Δv is small increment of the argument (the analysis step of 1.5 Â 10 16 Hz); lgε(v) and lgε(v + Δv) are molar absorption coefficients at certain frequencies; and m is the number of spectrum bands.
The dependencies of IP and EA on the μ-factor for polycyclic aromatic hydrocarbons (PAH) of various classes (Figures 2 and 3) are established [20]. In addition, the dependencies of IP and EA on IACP for oxygen-containing compounds (alcohols, aldehydes, ketones) (Figures 4 and 5) are established [21].
IP and EA of organic molecules and PAH of different origin are presented in Tables 5 and 6.
EA and IP of organic molecules and semiconductors of different origins are presented in Tables 2 and 3.
Thus it is established that IP and EA of PAH, calculated by RHF 6-31G** and DFT methods, have the IACF dependence and μ-factor. These dependencies allow simplification of the estimations of IP and EA of organic molecules and PAH of different origin (Tables 5 and 6).
Thus, new methods for determining characteristics of electronic structure of different molecules and organic semiconductors are developed.
Subsequently, dependence (14) was confirmed by the study of IP and EA for various classes of sulfur and nitrogen organic compounds, organic dyes, amino acids, and biological fluids [24].
In studies [2][3][4] for very complex multicomponent systems, the problem of determining the electronic structure and, consequently, chemical activity was solved.   The characteristics of the chemical activity can be determined from the electron absorption spectra simplification. The authors introduced new values: effective IP and effective electron affinity [30]. The effective IP and EA are the averaged potentials of ionization and the electron affinity of the radiation-absorbing components.
They allow to estimate the electron states of multicomponent and highmolecular substances, such as heavy residual resins of oil processing, highmolecular mixtures, and others.
Determining the electronic structure of materials and nanomaterials is an important problem of molecular electronics. For this, EPS was used. This application of EPS to determine the electronic structure of high-molecular compounds of petroleum (petroleum asphaltenes) was proposed in our previous works (Dolomatov et Table 6. Calculated values of IP and EA.
The asphaltenes are complex substances that can be found in crude oil, bitumen, and high-boiling hydrocarbon distillates. The asphaltenes are composed mainly of polyaromatic and heterocyclic compounds with traces of vanadium and nickel, which are in porphyrin structures. The electronic structure of asphaltenes has not been researched enough. The aim of research was to define the electronic structure of various asphaltenes. We have used the EPS methods. Some of the results are shown in Table 7.
Thus for asphaltenes, IP is in the interval from 4.37 up to 6.41 eV and EA differs from 1.82 to 2.66 eV. The size of energy band gap from 1.93 to 3.85 eV indicates that oil asphaltenes belong to amorphous, compensated, wideband semiconductors. The experiments for band gap estimation of the asphaltene molecules were confirmed by electronic structure computing with ab initio methods. The main deduction from this research is that oil asphaltenes can be used as organic semiconductors.

Effects of dependence of ionization potentials and electron affinity with color characteristics
The research [7] (co-author Dr. Shulyakovskaya D. and Dr. Yarmuhametova G.) established the phenomenon of the relationship between the energy of the molecular orbital, which characterizes the IP and EA, and color properties.
where Е is energy of the boundary molecular orbital (IP or EA), eV; α 1 and α 2 are empirically determined coefficients eV; q is one of the color characteristics (CCs) for standard light source A, B, C, or D; and CCs can be represented in one of the international color measurement systems (e.g., color coordinates or chromaticity coordinates in XYZ or RGB systems). The color coordinates of polycyclic aromatic hydrocarbons in the XYZ system are shown ( Figure 6). These coordinates are calculated in the visible region of the transmission spectra of hydrocarbon solutions according to the formulas (2)- (7).
Several classes of compounds, including PAH, were studied by dependence (25). The corresponding coefficients for IP and EA are presented in Tables 8 and 9. As can be seen from the tables, the accuracy of the assessment of ionization potentials and electron affinity is satisfactory. Thus, the effect of the relationship between IP and EA on the color characteristics can be used to simultaneously measure these physical quantities.  Table 7.
The characteristics of the electronic structure of asphaltenes by EPS method.  The dependence of the IP on the chromatic coordinate-Z in the XYZ system for PAH based on three and five linear annular benzene rings and from the perilene series is shown in Figure 7.
The IP and EA values for various organic molecules obtained by the dependence (11) are confirmed by various modifications of quantum DFT and ab initio methods. In addition, the values of IP were estimated by photoelectron spectroscopy. The results are shown in Tables 10 and 11.
From the received results, it follows that the equation is distributed to substances with IP < 9.8 eV, i.e., it covers the majority of organic substances.  Table 9.
Coefficients of dependence (25) for EA by PAH [4,7].  Thus, it can be concluded that the effects (21)-(25) discovered by us allow us to estimate the energy levels of quantum systems with sufficient accuracy. This is important for the study of multi-electron systems in molecular electronics and nanotechnology and chemistry such as single molecules, atomic clusters, and highmolecular systems. From here it follows that electronic spectra and color characteristics can be applied to the definition of various characteristics of substances.  Table 11. Results of determining of the first ionization potentials of some organic semiconductors [7].

Normal and pathological color characteristics of human blood components
This cycle of works is described [22][23][24][25][26] and executed together with Dr. N. Kalashchenko and Dr. S. Dezortsev. The experiments were conducted at the Ufa Medical University and the Republican Clinic named after Kuvatov (Ufa, Russia).
Colorimetric studies of blood are actively used in medicine [31], criminal law [32], and the food industry [33,34]. In medical practice, colorimetric methods are used to determine the hemoglobin concentration in the blood of a patient (the color index) [35]. Today a rather exact (AE1%) cyanomethemoglobin photometric method is used everywhere, in which cyanomethemoglobin is determined at a wavelength of 540 nm after preparation of a working solution of the blood in Drabkin reagent. Various modifications of this method do not change its essential physical nature [6]. Furthermore, spectral analysis in the visible region has been used to determine oxyhemoglobin and other hemoglobin-containing compounds from the absorption spectra of blood and its solutions [36]. Despite this, the quantitative colorimetric characteristics of blood have not been studied before.
The aim of this work was to study the color characteristics of hemolyzed blood, plasma, and serum from donors in the visible range of the absorption spectra by standard CIE methods (International Commission on Illumination, 1964).
The basic color characteristics (lightness and chromaticity coordinates) determine the position of the color of the specimen in an arbitrary color space and are found by the CIE method [11,12].
The familiar spectrophotometric method for color measurements involves measuring the spectral power distribution of the radiation followed by calculation of the color coordinates by multiplying the determined spectral power distribution function times the three color-matching functions and then integrating the products. For the spectral power distribution function of the source E(λ), the spectral transmittance function τ(λ), and x(λ), y(λ), and z(λ) (the color-matching functions) and the color coordinates X, Y, and Z are determined by integration over the wavelength range for visible radiation 380-760 nm. In practice, integration is replaced by summation over the interval dλ (from 5 to 10 nm), since the spectral functions under the integral sign are usually not easily integrated: The spectral power distribution and the spectral transmittance curve are measured by separating light into a spectrum, such as in a spectrophotometer or monochromator. The color-matching curves are specified as tables of values of the specific coordinates in 10 nm steps. There are also tables of E(λ)x(λ) values for standard CIE light sources A, B, C, and D, characterizing the most typical natural (B, C, D) and artificial (A) illumination conditions.
The chromaticity coordinates are calculated using the formulas. x The coordinate Y characterizes the lightness (luminance) of the specimens.
The quantitative colorimetric characteristics of hemolyzed blood, plasma, and serum described by formulas (26) and (27) in the standard CIE method are connected with the transmittance or reflectance spectra and are integrated parameters determined over the entire visible region of the electromagnetic spectrum. So it is assumed that they carry information about the condition of the entire body. In our approach, blood and its components are considered as a single, indivisible lightabsorbing system.
The experiment. The objects of investigation were solutions of hemolyzed blood and solutions of plasma and serum (prepared from that blood) of the same concentration from 100 male and female donors (in different blood groups and age groups) and from 95 patients who were assigned to three arbitrary groups: (I) 41 patients with purulent diseases (osteomyelitis, purulent fistulas, gonitis), (II) 41 resuscitated patients (acute myocardial infarction, acute cerebral circulatory collapse, chronic cardiac insufficiency), and (III) 13 patients with cirrhosis of the liver. We determined the color characteristics of the "average" donor (without separating the donors according to blood, sex, and age groups) and compared them with the analogous characteristics of patients from the different groups. The blood for the studies was drawn at a blood donation center and in clinical departments by standard procedures [37].
The spectra of the solutions of hemolyzed blood, plasma, and serum of concentration 2.5 vol.% (1.40) were taken in quartz cuvets with thickness of the working layer of liquid equal to 10 mm at room temperature, on an SF-2000 spectrophotometer in the range 200-1000 nm in 20 nm steps. As the solvent and the reference solution, we used distilled water for injection, which is optically neutral under the experimental conditions and is the natural physiological solvent in the human body. The hemolyzed blood was prepared using a standard heparin solution, and the plasma was prepared using the preservative Glyugitsir.
In addition to the averaged values of the color coordinates and the lightness value, we calculated the standard deviation, the confidence interval for significance level α = 95%, and the coefficient of variation. Figure 8 shows the averaged spectra for the hemolyzed blood, plasma, and serum from the patients in all three examined groups compared with the corresponding averaged spectra of the donors. There are clear differences between the different groups of patients.
For the plasma and serum, over the entire studied region, the group spectra for the patients lie higher than the averaged spectra of the donors, and their positional order is consistent: the averaged spectrum for patients with purulent diseases lies above the averaged spectrum for the donors, the averaged spectrum for the resuscitation patients lies above that spectrum, and the averaged spectrum for patients with cirrhosis of the liver lies even higher. Probably such positioning of the spectra reflects the severity of the general condition of the patients, if we assume that cirrhosis is the most severe condition for the patients with the least likelihood of recovery. We do not observe such a dependence for the hemolyzed blood: the averaged spectrum for the patients with purulent diseases lies below the averaged spectrum for the donors (Figure 9). Table 12 gives the averaged color coordinates and lightness for the donors and each group of examined patients, calculated for the solutions of blood, plasma, and serum as a single light-absorbing system according to the standard CIE method. For the donors, the chromaticity coordinate x varies from 0.320 AE 0.001 (for serum and plasma) to 0.630 AE 0.008 (for hemolyzed blood). The coefficients of variation in this case also decrease from 4.7 for blood down to 1.3 for serum. The chromaticity coordinate y for the donors has similar values: 0.320 AE 0.002 for plasma and serum and 0.340 AE 0.003 for blood. The coefficients of variation for y steadily decrease from 3.2 for blood down to 2.0 for serum. The parameter z for the donors is higher for serum and plasma (0.360 AE 0.003) than for blood (0.030 AE 0.005). The coefficient of variation for this parameter is maximum for blood (67.6) and minimum for serum (2.5). The lightness, as expected, has the maximum value (84.88-1.54) for serum and the minimum value (11.55-0.67) for hemolyzed blood. For plasma, this parameter is close to the value typical of serum.
In determining the color range (see Figure 9a) for the dilute solutions (1.40) of hemolyzed blood, plasma, and serum from the donors, the color range of blood falls within the red region of the spectrum; the range for plasma and serum falls within the yellow region with lower saturation, which supports the correctness of our experiments and calculations. The corresponding regions for the color range for the patients cover a larger area than for the donors (Figure 9b). In order to better visualize the results obtained, we calculated the color coordinates for the studied specimens with correction for concentration. All the points for the donors lie within the yellow-orange region with saturation of 30-50%, which corresponds to the visual observations.
The average values of the color coordinates for all the patient groups (see Table 12) are virtually no different from the averages for the donors except for  *Note: I, II, and III indicate the arbitrary groups of patients. Table 12.
patients with cirrhosis of the liver, for which the bilirubin blood concentration sharply increases. As a result of this, the plasma and serum take on a saturated orange color, which is reflected in the spectra and accordingly in their color characteristics. For the same reason, the lightness of the plasma and serum for these patients is much lower (by almost a factor of two) than for the rest. However, we do not observe sharp differences in the spectra of the hemolyzed blood. The lightness of the plasma and serum depends on the condition of the patients. Thus the average values of this parameter for the plasma decrease as the severity of the diseases increases: from 78.94 for the donors up to 67.82 for septic patients, 63.04 for resuscitated patients, and 36.66 for patients with cirrhosis of the liver.
In the future, we performed new experiments in which the possibility of diagnosis of liver cirrhosis by color characteristics of blood was considered [23].  Table 13. The chromaticity coordinates (x and y) of patients with liver cirrhosis and healthy people [25].  Table 14.

Statistical indicators
The statistical characteristics of chromaticity coordinates for blood plasma of healthy individuals and patients with liver cirrhosis in the XYZ system [25].
The color characteristics of the samples of blood plasma were calculated after processing spectra. The selective figures of the chromaticity coordinates of patients with liver cirrhosis and healthy people are shown in Table 13.
Then the statistical analysis of the data was made. The basic statistics for all the investigated samples are shown in Table 14.
Totalities of samples have a distribution close to normal and similar values of dispersion; therefore the t-test can be used to assess the reliability of the results. T of t-test for the chromaticity coordinate x was 10.57, for y-12.9. The critical value of t for confidence probability p = 0.999 is 3.5, which is much smaller than the obtained results. Consequently, the differences between chromaticity coordinates of groups of patients and donors were statistically significant.
The differences between the color characteristics of blood plasma of patients with liver cirrhosis and healthy subjects are shown in Figure 10.
Colorimetric method established that a healthy person is characterized by the following indicators of chromaticity coordinates: x ¼ 0:32 AE 0:001, y ¼ 0:32 AE 0:002: Patients with liver cirrhosis are characterized by the following color characteristics: x = x = 0.352 AE 0.006 y = 0.356 AE 0.005.
Having made the statistical processing, the data revealed that color characteristics of blood plasma of patients with liver cirrhosis differ from color characteristics of blood plasma of healthy people with a high degree of reliability.
Investigation of human blood plasma by colorimetric methods can be used to express diagnosis of liver cirrhosis. A healthy person is characterized by the following indicators of chromaticity coordinates: x = 0.32 AE 0.001, a = 0.32 AE 0.002. Figure 10. Blood plasma of patients with liver cirrhosis and healthy people on the chromaticity diagram of system XYZ (Source C) [24,25].