Secondary Metabolites of Mycoparasitic Fungi Secondary Metabolites of Mycoparasitic Fungi

Mycoparasitic fungi, fungi preying on other fungal species, are prolific producers of volatile and non-volatile secondary metabolites. Several secondary metabolites are produced during mycoparasitism to weaken the host and support attack and parasitism. Further, evidence accumulated that some secondary metabolites also act as communication molecules. Besides their antagonistic activity, several fungal mycoparasites exhibit beneficial effects on plants and some of their secondary metabolites have plant growth-promoting and defense stimulating activities. As many secondary metabolism-associated gene clusters remain silent under standard laboratory conditions, the full variety as well as the underlying biosynthetic pathways employed by fungal mycoparasites for second- ary metabolite production still await clarification. Nonetheless, the variety of currently known secondary metabolites and their range of activities is impressive already and they exhibit a great potential for agriculture, pharmacology and other industrial applications.


Introduction
Mycoparasitic relationships, where a predatory fungal species gains nutrients on the expense of a host fungus, are widespread within the fungal kingdom. By the modalities of this nonmutual relationship, biotrophic and necrotrophic mycoparasitic fungi with different gradations within this classification (contact, invasive or intracellular necrotrophic; haustorial or fusion biotrophic) can be distinguished [1]. Biotrophic mycoparasites co-exist and nourish on their living host in a balanced way and are specifically adapted to one or few host species. In contrast, necrotrophic fungi destructively invade and kill a broad range of hosts to gain nutrients from the remains of their prey [2]. Mycoparasitic fungi are prolific producers of a plethora of volatile and non-volatile secondary metabolites, favoring their ecological fitness and survival under certain environmental conditions. For example, the excretion of siderophores -affecting high affinity iron chelation -is strongly up-regulated under iron-limiting conditions [3] and several antimicrobial metabolites empower the successful perseverance within the ecological niche [4]. The mycoparasitic lifestyle obviously substantiates the overrepresentation of secondary metabolism-associated genes and the extensive excretion of a variety of secondary metabolites [2] enabling the fungus´ successful access to its prey as well as its thriving persistence in or assassination of the host. Furthermore, selected fungal secondary metabolites are known to exhibit beneficial effects on plants: They may promote vitality and growth of roots and shoots, enhance the resilience against abiotic stress factors and prime the plants immune system (induced systemic resistance; ISR) thereby enhancing its resistance and survival in case of prospective infections with pathogens [5]. In recent times, evidence accumulated that some secondary metabolites also act as communication molecules over species boundaries [6,7].
A great diversity of mycoparasitic species exists in the fungal kingdom, especially within the order Hypocreales [8]. In this aspect Trichoderma (teleomorph Hypocrea), a worldwide abundant, diverse fungal genus, is one of the best-studied examples [2]. Trichoderma comprises necrotrophic mycoparasitic species like Trichoderma atroviride or Trichoderma virens, which are successfully applied in agriculture as biocontrol agents against plant pathogenic fungi of crop plants. Further they are reported to promote plant growth, vitality and systemic resistance via priming the plants' immune system. The genomes of several Trichoderma species have been sequenced and analyzed revealing the ancestral mycoparasitic lifestyle of these fungi [9,10]. The second largest lineage of mycorparasites within the Hypocreales is the genus Tolypocladium. Tolypocladium comprises, besides of some entomopathogenic species like Tolypocladium inflatum, mostly mycoparasitic species like the widespread on northern hemisphere Tolypocladium ophioglossoides which mycoparasitizes with a narrow host range on truffles of the genus Elaphomyces [11,12]. In contrast to Trichoderma, the genus Tolypocladium exhibits an ancestral entomopathogenic lifestyle and developed to a mycoparasite by host jumping. The genomes of Tolypocladium species are rich in secondary metabolite gene clusters of which some, like the clusters for the production of peptaibiotics, seem to be exclusive to mycoparisitic lineages [12]. A further well-investigated mycoparasitic fungus is Escovopsis weberi. E. weberi is a contact necrotrophic mycoparasite on Leucoagaricus sp. in leaf-cutting ant agriculture [13]. As foraging for leaves causes considerable economic damages in neotropic agriculture by defoliation of a wide variety of crop plants, E. weberi would be a suitable biocontrol agent as it causes a breakdown of the fungal feeding structures, thereby starving out the ant colony leading to a collapse of the whole system [14]. Like other very specialized mycoparasites, the E. weberi genome exhibits a reduced size and content, but very unique secondary metabolite clusters for host attack, facilitating the excretion of fungicidal substances which can break down the host mycelia even without contact [15,16]. Further examples of secondary metabolite analysis on mycoparasitic species include Stachybotrys elegans, a potential biocontrol agent against plant pathogenic Rhizoctonia solani [17] as well as Coniothyrium minitans and Microsphaeropsis ochracea co-culture antagonizing the plant pathogenic Sclerotinia sclerotiorum [18].
A characteristic trait of filamentous fungi is that their secondary metabolism-associated genes are mostly situated within subtelomeric regions of the chromosomes in large biosynthetic gene clusters present in the genomes in significantly greater numbers than secondary metabolites currently identified [19]. The unique and often uncommon biosynthetic pathways are mostly characterized by signature enzymes, often also transcription factors and transporters present in the respective gene clusters, which enable the secondary metabolite synthesis starting from simple precursors gained from primary metabolism like amino acids and acetyl-CoA [20]. Most common core enzymes are non-ribosomal peptide synthases (NRPSs), polyketide synthases (PKSs) and terpene-synthases or -cyclases [4]. In necrotrophic mycoparasitic species like T. atroviride and T. virens, genes for the biosynthesis of secondary metabolites are enriched compared to the only weakly mycoparasitic relative Trichoderma reesei. Within the two mentioned strong mycoparasites nearly half of all secondary metabolism-associated genes are positioned on non-syntenic regions in the genome and do not exhibit orthologs in the respective other species [21]. Depending on the species, environmental conditions, and even the strain, a plethora of different compounds is derived by the genus Trichoderma [2,4], awakening hope on the detection of new substances. Since resistance development to the currently applied substances constitutes an increasing problem in agriculture and medicine, the need for environmentally sustainable biological control of pathogens and the discovery of novel antagonistic substances is essential. Secondary metabolites of mycoparasitic fungi could contribute to the solution.

Non-ribosomal peptides
Non-ribosomal peptides (NRPs) are synthesized by NRPSs, enzymes that characteristically consist of multiple domains synthesizing the peptide in one by one steps. Characteristic for NRPSs are the core domains for adenylation, thiolation and condensation. The generated NRPs are very diverse: they mostly comprise of proteinogenic and non-proteinogenic amino acids, can be linear or branched to cyclic with a varying length. After their synthesis outside of the ribosome, they frequently pass extensive secondary modifications. Many fungal NRPs have high economic and/or ecologic value like β-lactam antibiotics, the immunosuppressant cyclosporine A but also mycotoxins like gliotoxin.
The occurrence of NRPS genes is enriched within the genome of mycoparasitic T. atroviride for 60% and in T. virens for 180% to the wood-degrading T. reesei [21]. Further, the functional involvement of NRPS and PKS in the mycoparasitic interaction was supported by deletion experiments of the T. virens 4-phosphopanteteinyl transferase-encoding gene (ppt) -which is essential for NRPs and PKSs activation -resulting in mutant strains defective in mycoparasitism and induction of systemic resistance (ISR) in plants [22]. The main NRPSs derived metabolites in Trichoderma species are peptaibiotics, epipolythiodioxypiperazines and siderophores.

Peptaibiotics
Peptaibiotics are mostly linear to rarely cyclic polypeptides, with a size of 0.5-2.1 kDa consisting of 4-21 residues. Characteristic for peptaibiotics is the inclusion of the non-proteinogenic amino acid α-aminobutyric acid (Aib). By module-skipping one NRPS is frequently capable of synthesizing a whole set of peptaibiotics [23,24]. According to their sequence alignment and structure, peptaibiotics can be divided into several sub-clades: peptaibols, lipopeptaibols, lipoaminopeptides, cyclic peptaibiotics and two very special, small categories [25]. Because of their unusual synthesis and appearance, they are not included in regular protein databases, but in the "Comprehensive Peptaibiotics Database" [25].
Peptaibols are solely described for filamentous fungi exhibiting a mycoparasitic lifestyle, with a high abundance of over 80% of all known substances being derived from Trichoderma species [25]. Peptaibols are linear peptides, which besides of Aib include a characteristic acetylation of the N-terminus and a 1.2-amino-alcohol at the C-terminus. The first peptaibols, suzukacillin and alamethicin, have been described in the 1960s [26]. As demonstrated for alamethicin, the amphipathic character of peptaibols allows the voltage-dependent formation of helical structures acting as ion channels, thus spanning and permeabilizing the cell membrane and leading to cytoplasmic leakage and cellular breakdown [27].
Whereas all Trichoderma strains produce peptaibols, some substances are synthesized in a species-or even strain-specific manner [25]. For instance, in five different biocontrol agents containing species from the Trichoderma harzianum complex, peptaibols were the dominant secondary metabolites including three new and recurrent major groups present in all formulations [28]. Peptaibols play an important role in the mycoparasitic interactions as well as in induction of ISR in plants via up-regulation of the jasmonic acid and salicylic acid synthesis [29,30]. In T. harzianum, peptaibols synergistically act together with hydrolytic, cell wall degrading enzymes on the cell wall destruction of the host fungus [31,32]. Other mycoparasites such as T. ophioglossoides and E. weberi also comprise peptaibiotics-associated gene clusters, which are absent in plant-and entomopathogenic lineages of Hypocreales, suggesting the restriction of these genes to mycoparasitic species, further indicating their importance in the mycoparasitic interaction [11,15]. The antifungal activity of the peptaibol trichokonin from Trichoderma pseudokongii caused extensive apoptosis by loss of the mitochondrial transmembrane potential resulting in apoptotic cell death in Fusarium oxysporum [33]. Similar evidences suggest a major involvement of peptaibiotics in mycoparasitism, substantiated by reports of antifungal action of peptaibols secreted by Clonostachys rosea against S. sclerotiorum [34], or Sepedonium tulasneanum against Botrytis cinerea and Phytophthora infestans [35].

Epipolythiodioxypiperazines
Epipolythiodioxypiperazines (ETPs) are characterized by the presence of an inter-or intramolecular disulfide bridge and a diketopiperazine core. The toxicity of ETPs lies in the disulfide bridging which is facilitating the inactivation of proteins by conjugation and the elicitation of reactive oxygen species (ROS) [36]. The best known substance of this class is gliotoxin derived from T. virens Q-strains [37]. T. virens P-strains antagonize Pythium ultimum and do not produce gliotoxin but the terpenoid gliovirin, whereas Q-strains affect R. solani but not vice versa [38]. C. rosea also produces ETPs like verticillin A and gliocladines involved in the antagonism on nematodes [39] and glioperazine exhibiting antibacterial properties [40]. Whereas the role of gliotoxin (cluster comprising of 12 genes) as a virulence factor in human Aspergillus fumigatus infections and the self-protection via the gliT gene product of the biosynthetic gliotoxin cluster is well investigated, there is little and partially adverse information on the role of ETPs in biocontrol [36].
The weak mycoparasitic T. reesei exhibits an incomplete gliotoxin cluster whose genes were not expressed during confrontation with R. solani [4], whereas highly mycoparasitic T. atroviride does not contain a gliotoxin cluster [2]. The gliotoxin gene cluster of T. virens Q-strains consists of eight genes encoding the core enzyme gliP -an NRPS dioxypiperazine synthase -whose expression was induced during confrontation with R. solani [4]. Deletion of gliP resulted in gliotoxin production-deficient mutants, going hand in hand with a significantly reduced induction of ISR in cotton seedlings and antagonistic action against P. ultimum and S. sclerotiorum. Adversely, the mutants' antifungal activity against R. solani remained unaltered [41]. The involvement of ETPs in mycoparasitic interactions stays unresolved and seems to depend on the combination of several -largely unknownfactors like synergistic interactions with other metabolites or enzymes [42], environmental conditions, the species, strain and even the host organism.

Siderophores
Siderophores of fungal origin are high affinity iron chelating, linear to cylic oligomeric secondary metabolites mostly characterized by a N5-acyl-N5-hydroxyornithine basic unit [3]. Several siderophores are derived by one NRPS and post-synthetic subsequent modification [43]. As bio-available iron is rare in natural habitats, but an essential trace element to most organisms, efficient chelation, uptake and storage mechanisms for iron play an important role in competition and perseverance, especially within dense microbial communities like in soil [44]. Siderophores are important metabolites in the response against oxidative stress in several fungi like Aspergillus nidulans, A. fumigatus, Cochliobolus heterostrophus, Gibberella zeae and T. virens; furthermore, they play a role in conidial germination and sexual development [4,[45][46][47].
Evidences accumulate that siderophores act in biocontrol as virulence factors against other microbes during iron competition. Further, they promote plant growth by the reduction of oxidative stress: in biocontrol of Fusarium wilt disease by Trichoderma asperellum strain T34, the tomato plants exhibited reduced numbers of infected roots and a decrease in iron-associated abiotic stress [48]. The over-representation of siderophores in Trichoderma hamatum strain GD12 was reported to beneficially influence the biocontrol of S. sclerotiorum and plant growth promotion in lettuce [49]. More direct evidence for an involvement of siderophores in mycoparasitic interactions exists in C. minitans: the expression of CmSIT1, a gene-mediating siderophore-iron transport, not only enhanced antifungal abilities but also reduced growth [50].

Polyketides (PKs)
Polyketides (PKs) are derived from simple building blocks like acetyl-CoA or malonyl-CoA via consecutive PKS-mediated decarboxylative condensation and subsequent post-synthetic modification. Fungal PKSs are complex multi-modular enzymes, which obligatory include a characteristic ketoacyl-CoA-synthase (KS), an acyltransferase (AT) and an acyl-carrier (ACP) domain [20]. The structurally diverse PKs are the main class of secondary metabolites derived from fungi. The spectrum of substances ranges from spore pigments over antibiotics to toxins [2].
The T. virens and T. atroviride genomes are enriched for about 60% in PKS genes compared to T. reesei [21]. The C. rosea genome even exceeds this number with a total of 31 PKS genes [51], whereas the T. ophioglossoides genome comprises 15 PKSs [11]. The TMC-151 type PKs derived from C. rosea exhibits antibacterial properties [52], whereas T. ophioglossoides produces two antifungal and antibacterial substances: the polyketide ophiocordin and the NRPS-PKS hybrid enzyme-derived ophiosetin [11,53,54]. The deletion of pks4 -encoding an orthologue of the aurofusarin and bikerfusarin forming PKSs of Fusarium spp. -in T. reesei caused extensive changes in morphology as well as physiology and metabolism. In ∆pks4 mutants, the pigmentation of conidia and the generation of teleomorph structures were inhibited, and the stability of the conidial cell wall was reduced. Pks4 deletion decreased T. reesei's antagonistic abilities in confrontation assays, lowered its antifungal effect mediated by water soluble and volatile metabolites and altered the expression pattern of other PKSs [55]. It seems that also within this metabolite class, the effects are more diverse and global, than hitherto expected.

Terpenoids
Terpenoids are synthetized from the acetyl CoA-derived C-5-isopentenyl-diphosphate intermediates isopentenyl-and dimethylallyl-diphosphate. The C-5 units are subsequently processed via head-to-tail condensation by prenyl synthases and are post-synthetically modified by various enzymes resulting in different terpenoids originating from very few C-5 precursors [56]. Terpenoid biosynthetic clusters are characterized by the presence of a terpene cyclase gene [4]. Terpenoids are volatile to non-volatile substances constituting the highest abundant natural products on earth [37]. Terpenoids of fungal origin comprise phytohormones, mycotoxins as well as antibiotics and antitumor substances.
The C. rosea genome contains eight terpene synthase genes [51] and E. weberi comprises an expansion of six genes for terpene synthases in its genome, of which three lie within unique biosynthetic secondary metabolite clusters [15]. The majority of secondary metabolites of S. elegans secreted during mycoparasitizing R. solani are trichothezenes and atranones belong to the terpenoid class of secondary metabolites [17]. The T. virens genome comprises an enrichment of terpene cyclase genes compared to T. atroviride and T. reesei [57]. The production of several terpenoids was proven for Trichoderma species [58,59], whereas their biosynthetic pathways mostly still remain obscure. The putative terpene cyclase vir4 is well-researched and only present in T. virens, but not in T. reesei or T. atroviride. Analysis of a mutant which exhibited defects in antibiotic production, a lack of viridin and viridiol synthesis and an under-expression of most of the genes of the vir4 cluster evidenced that the cluster is involved in viridin biosynthesis [60]. Generation of a vir4 deletion mutant and metabolic screening validated its involvement in terpene biosynthesis; the terpene cyclase gene vir4, however, turned out not to be involved in viridin or viridiol biosynthesis but in the synthesis of more than 20 volatile sesquiterpenes [61]. The involvement of terpenes in mycoparasitism relies unresolved, but there are hints: it is probable that genes underlying the mevalonate pathway also influence terpene synthesis. The hmgR gene codes for the glycoprotein 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, which processes HMG-CoA to mevalonic acid. Accordingly hmgR-silenced mutants of T. harzianum exhibited decreased antifungal abilities [62]. Deletion of the trichodiene synthase genes tri4 and tri5 in Trichoderma arundinaceum resulted in a loss of harzianum A production, a reduced antagonism against host fungi and a decreased ISR in tomato plants [63,64]. Expression of the T. arundinaceum tri4 and/or tri5 genes in T. harzianum mainly influenced plant wealth and defense by induced production of trichodiene and 12, 13-epoxytrichothec-9-ene (EPT) [65,66], whereas tri5 overexpression in Trichoderma brevicompactum boosted the excretion of antifungal trichodermin [67,68].

Ecology and regulation of secondary metabolism in mycoparasitic fungi
Ancestral and recent lifestyles fundamentally influence the existence as well as the expression of secondary metabolite genes and clusters up to the species or even strain level. The transcriptional responses of T. reesei, T. atroviride and T. virens -which all share an ancestral mycoparasitic lifestyle -to the confrontation with R. solani illustrates this fact very well. All three species exhibit few common metabolic responses but autonomous and specific strategies in defeating their opponent. Both potent mycoparasites, T. atroviride and T. virens, attack their hosts in the stage before physical contact, but with distinct strategies of antibiosis. T. virens offends mostly via the NRP biosynthetic pathway for gliotoxin synthesis, whereas T. atroviride combats mainly via the PKS biosynthetic pathway, as well as the excretion of 6-pentyl-α-pyrone (6-PP) -a volatile organic compound (VOC) with antifungal and plant growth-promoting properties [2]. Conversely, the transcriptional response of the only slightly mycoparasitic T. reesei is more defense-related and targeted on the excretion of cellulases, hemicellulases and ribosomal proteins before hyphal contact [69]. The long-term specialized lifestyle and co-evolution of E. weberi with its host in its relatively demarcated ecological niche facilitated the loss of a manifold of genes leading to a more or less obligatory mycoparasitism with limited growth and viability without the presence of the host. Hence, the E. weberi genome demonstrates a high degree of specialization, with a unique secretome containing an unusually high content of over 50% of proteins with unknown function. Further, the genome contains only 20% homologs with the closely related T. atroviride, T. virens and T. reesei and only 12% of the 1066 unique genes exhibit homology with proteins in the whole subdivision of Pezizomycotina [15]. Several environmental cues like temperature, light, carbon, nitrogen, pH and competing or synergistic organisms are known to influence the transcriptional regulation of secondary metabolism-associated gene clusters (Figure 1). Suboptimal environmental conditions thereby often facilitate and promote transcriptional activation or transcriptional reprogramming events [70]. In media containing chitin or B. cinerea cell walls, the predicted cutinase transcription factor 1 encoding gene of T. harzianum (Thctf1) was up-regulated. Thctf1 deletion mutants exhibited reduced antagonistic and antifungal ability, and the mutant strain did not synthesize two 6-PP derivatives, indicating a role of Thctf1 in secondary metabolism of T. harzianum [71]. Furthermore, the overexpression of the gene encoding multiprotein bridging factor 1 (Thmbf1) of T. harzianum -a transcriptional co-activator of Thctf1 -negatively regulated the antifungal abilities, as well as the expression of VOCs [72].
Like known for the production of mycotoxins in non-mycoparasitic species [73], secondary metabolite production in mycoparasitic fungi is governed by heterotrimeric G protein signaling and the associated cAMP-pathway, as well as mitogen-activated protein kinase (MAPK) cascades [74,75]. T. atroviride mutants, lacking the MAPK-encoding gene tmk1 showed an enhanced production of peptaibols and of 6-PP [74]. First evidence for a positive regulation of the secondary metabolism by cAMP signaling came from T. virens ∆tac1 mutants bearing a deletion of the adenylate cyclase-encoding gene. The mutants were unable to offend Sclerotium rolfsii and R. solani, but showed a clear inhibition zone in direct confrontation with Pythium sp., pointing to a host-dependent expression of secondary metabolism-associated genes. Further, the mutant exhibited a diminished production of secondary metabolites like viridiol and a reduced expression of secondary metabolism-associated genes [75]. Prior to that, similar results were obtained for T. atroviride ∆tga1 mutants missing the subfamily I Gα protein-encoding gene. Deletion of tga1 led to a complete loss of overgrowth and mycoparasitism of different preys during direct confrontation and a decrease of 6-PP and sesquiterpene production as well as chitinase gene transcription. Despite the reduction in chitinase and 6-PP accumulation, the ∆tga1 mutant caused a strong growth inhibition of prey fungi in the interaction zone mediated by yet unidentified low molecular weight antifungal metabolites, thereby evidencing opposite roles of tga1 in regulating the biosynthesis of different antifungal substances in T. atroviride [76]. Similar to ∆tga1 mutants, transformants bearing a deletion of the subfamily III Gα protein-encoding gene tga3 were unable to overgrow and lyse prey fungi. However, absence of the adenylyl cyclase-stimulating Tga3 protein led to significantly reduced antifungal activity [77]. The global regulation of secondary metabolism and morphogenesis by the heterotrimeric VELVET protein complex, consisting of the S-adenosylmethionine-dependent methyltransferase LaeA and the velvet proteins VeA and VelB, was first described in A. nidulans [78]. Deletion of the laeA orthologue lae1 in T. atroviride led to a loss of mycoparasitic abilities in direct confrontation and a major reduction in the synthesis of 6-PP and water-soluble secondary metabolites. Further, the expression of eight mycoparasitism-related genes was decreased in the mutant. The deletion of vel1 -the veA orthologue -in T. virens caused defects in overgrowth and offense against the host in direct confrontation as well as in bioprotective plant interaction, accompanied by a decrease in the expression of several secondary metabolism-associated genes [79,80].

Cross-talk by and response to secondary metabolites in mycoparasitic interactions
In bacteria, it has been shown that at sub-inhibitory concentrations antibiotics serve as mediators of microbial communication and interaction with one of the outcomes being the production of cryptic metabolites [81]. Accordingly, the interaction with other fungi may shape the secondary metabolite profile of a specific fungus, making co-cultures a valuable tool for eliciting the activation of silent secondary metabolism-associated gene clusters.
Studies on the mutual effects of secondary metabolites produced during mycoparasitic interactions are rare however. Trichoderma-derived 6-PP was shown to suppress the synthesis of the Fusarium mycotoxins fusaric acid and deoxynivalenol (DON) [82][83][84][85], suggesting that 6-PP acts as communication molecule that elicits biological responses in the interaction partners. On the other hand, fusaric acid and DON modulate 6-PP production as well as chitinase gene expression in T. atroviride and recent studies provided evidence that Fusarium mycotoxins induce defense mechanisms in mycoparasites such as T. atroviride and C. rosea which results in mycotoxin detoxification [59,86]. C. rosea was shown to open the ring structure of zearalenone (ZEN), while Trichoderma spp. seem to convert ZEN into its reduced and sulfated forms and metabolize DON to deoxynivalenol-3-glucoside, a detoxification product of DON previously identified in plants [87,88]. In the interaction of the mycoparasite T. arundinaceum with B. cinerea, Botrytis-derived mycotoxins botrydial and botcinins attenuated trichothecene biosynthesis gene expression in Trichoderma while botrydial production was repressed by Trichoderma-derived harzianum A and aspinolide [89][90][91].
Co-culturing of mycoparasites with prey fungi simulates the conditions occurring during the mycoparasitic interaction in natural or agricultural systems and could hence encourage the production of secondary metabolites via communication and signaling molecules. Accordingly, pairwise interactions of Aspergillus niger, Fusarium verticilliodes and C. rosea led to metabolites that occurred in single cultures but were suppressed in dual cultures, and many new metabolites not present in single cultures were found in dual cultures [92]. Similar results were obtained in co-culturing experiments of T. harzianum and Talaromyces pinophilus with the accumulation of siderophores being induced in both interaction partners and the production of Talaromyces-derived 3-O-methylfunicone and herquline B being reduced. In addition, the novel substance harziaphilic acid was detected in the co-culture only [93].
Based on these studies, it is evident that secondary metabolites contribute to mycoparasitic interactions in various ways including inhibition of the activity or synthesis of mycoparasitism-relevant enzymes and other substances, by eliciting defense and detoxification responses or by triggering the production of cryptic metabolites. In most cases, however, information on the spatial distribution of the secreted substances is lacking and it is hence difficult to assign novel secondary metabolites specifically induced during the co-cultivation to its actual producer. Recently, mass spectrometry-based imaging (MSI) turned out as a valuable tool for in situ visualizing the dynamics and localization of small molecules released during microbial interactions [94]; however, reports on its application to mycoparasitic fungus-fungus interactions are still rare. By applying matrix-assisted laser desorption/ionization (MALDI)-based MSI for visualization and identification of secondary metabolites being exchanged during the mycoparasitic interaction of T. atroviride with R. solani [95], the diffusion of Trichodermaderived peptaibols toward the prey and the accumulation of Rhizoctonia-derived substances at the borders of fungal interaction was tracked. Monitoring of the T. harzianum interaction with the fungal phytopathogen of cacao plants Moniliophthora roreri by MSI lead to the detection of T39 butenolide, harzianolide, sorbicillinol and an unknown substance specifically produced in the co-culture with a spatial localization in the interaction and overgrowth zones [96].