Syntheses and Applications of Titanium Compound Nanofiber Thin Films

Syntheses, characteristics, wettability, and antibacterial activity of a titanium phos‐ phate nanobelt thin film and titanate nanofiber thin films formed on titanium plates are presented in this chapter. Extremely low values of 1.0 and 0.3 were recorded for antibacterial activity during the second and third rounds of antibacterial tests conducted without exposure to ultraviolet rays. The above results revealed that (1) a high level of antibacterial activity manifested with the sodium titanate nanofiber thin film due to photocatalysis effects with exposure to ultraviolet rays and that (2) the sodium titanate nanofiber thin film has a high level of antibacterial activity immediately after synthesis, even when they are not exposed to any ultraviolet rays. Only the sodium titanate nanofiber thin film indicating a high level of antibacterial activity is extremely peculiar because the antibacterial activity of the sodium titanate nanosheet thin film and nanotube thin film described above were 2.1 [10] and 1.7 [34], of the silver nanoparticles. No sodium was detected from the thin film after the silver ion exchange process, but silver was detected when the EDS analysis was conducted. A silver‐to‐titanium molar ratio of 0.638 was detected; this is greater than the was observed with silver nanoparticles/silver titanate nanofiber thin film, 94 ppm was observed with the silver nanoparticles/silver titanate nanotube thin film, and 68 ppm was observed with the silver particles/silver titanate thin film with a porous


Introduction
Vigorous efforts have been made in recent years to control the structure and morphology of ceramic nanoparticles. The formation of peculiar nanostructures and morphologies can expose a specific crystal surface or cause a high specific surface area or characteristic pore diameter distribution, which can result in the manifestation of various peculiar physical and chemical properties. Vigorous efforts have been made to form the thin film comprised of nanoparticles, which should help peculiar physical and chemical properties of nanoparticles manifest more efficiently. The dip-coating, spin-coating, and electrophoretic deposition methods are commonly used to form the thin film, but they tend to cause nanoparticles to be randomly deposited on substrates, and the adhesion between the particles and substrate is often not good. An alternative method to form the thin film involves forming a nanostructured metal compound thin film on a metal surface where the metal itself is used as the raw material. Because the metal compound grows crystals directly on the metal surface in the nanostructured metal compound thin film/metal composites, superior adhesion between the metal and the thin film can be expected. In addition, the particle arrangement can be controlled by aligning the crystal orientations of the particles to some degree. The superior electrical conductivity and mechanical properties of the metal and the peculiar physical and chemical properties of the nanostructured metal compound thin film can potentially provide a superior functional material that combines all such properties. Vigorous research efforts have been made on this aspect, particularly with regard to the synthesis of a nanostructured titanium compound thin film/ titanium composite. This is because titanium compounds such as titanium dioxide and titanate feature superior characteristics for a broad range of applications, e.g., photocatalysts [1], dyesensitized solar cells [2], lithium-ion batteries [3,4], and biomaterials [5,6]. Titanium dioxide and titanate are also extremely attractive because they are available at relatively low prices and are safe materials with no toxicity. Titanium dioxide and titanate have reportedly been used in such syntheses as the formation of a titanium dioxide nanotube thin film on a titanium metal surface with an electrochemical method (anodic oxidation of titanium metal) [6,7], the formation of nanostructured titanate (nanotube, nanofiber, nanosheet, etc.) thin films on titanium metal surfaces by the reaction of titanium metal in an alkaline aqueous solution, and their conversions into nanostructured titanium dioxide thin film [5,[8][9][10][11]. For example, known applications of composites that utilize the superior electrical conductivity of titanium include electrode materials of dye-sensitized solar cells [12], lithium-ion batteries [13], and sodium-ion batteries [14]. Superior electrical conductivity can be secured by using titanium dioxide or titanate with one-dimensional morphologies, such as nanorods, nanotubes, or nanofibers, while electrodes can be prepared without using binders or conducting agents. Applied research on field emission materials [15] and electrochromic materials [16] of composites is also underway. On the other hand, biomaterials are a known application that utilizes the superior mechanical properties and safety of titanium metal. Titanium and titanium alloys are used as artificial joints based on their mechanical properties and safety inside the living body. Using composites adds osteoconductivity derived from titanium dioxide or titanate and antibacterial properties to titanium and titanium alloys, which facilitates the safe adhesion of bones and artificial joints in a short period of time [5,6]. Antibacterial biomaterials are also desirable for medical sites; infections occur in 1-2% of artificial joint replacement surgeries and are a major problem [17,18]. The present chapter evaluated the synthesis and antibacterial properties of titanate nanofiber thin film/ titanium metal composites as an example application for biomaterials [10]. Various crystal structures such as Ti(HPO 4 ) 2 ·H 2 O and Ti(H 2 PO 4 )PO 4 ·2H 2 O are known to exist with titanium phosphate. Morphology controls to shape nanotubes [19], nanofibers [20], elongated hexagonal nanoplates [21], and so on are also being developed. Applications of titanium phosphate include not just fundamental research on topics such as ion exchange and intercalation reactions but also catalysts [22], selective absorbents for toxic substances (e.g., Pb 2+ , Cd 2+ ) [23,24], the support and release of drugs [25], sodium batteries [26], and solar cells [27]. However, examples of reports on such efforts are primarily about particles, and there have not been many examples of studies on thin films. In particular, there are only three reports on the synthesis of titanium phosphate thin film on the surface of titanium, including the research of the present author. Lu et al. [28] immersed titanium in a phosphoric acid aqueous solution and induced a hydrothermal reaction at 250°C, while Park et al. [29] also immersed titanium in a phosphoric acid aqueous solution and induced a hydrothermal reaction at 180°C to form Ti 2 O(PO 4 ) 2 (H 2 O) 2 nanorod thin films on the titanium surface. Park et al. [29] revealed that such a thin film has superior osteoconductivity. The present author added to these prior studies by discovering that titanium phosphate featuring a variety of crystal structures and morphologies is generated on the surface of a titanium plate immersed in an aqueous solution of hydrogen peroxide and phosphoric acid to produce a hydrothermal reaction [30]. This book is focused on nanofibers; this chapter narrows the focus to the titanium phosphate nanobelt thin film/titanium metal composite by introducing the formation mechanism and wettability.

Synthesis and wettability of a titanium phosphate nanobelt thin film
A titanium plate (20 mm × 20 mm × 0.5 mm) was scoured by using an abrasive paper and then immersed in 10 mL of an aqueous solution of 2 mol/L hydrogen fluoride for 10 min at room temperature to remove the surface-oxidized phase. It was then repeatedly washed with distilled water. A synthesis experiment of titanium phosphate thin film was performed with the derived plate. This experimental manipulation was performed in order to obtain a homogeneous titanium surface. This manipulation changed the morphology of the surface from that shown in Figure 1a to that shown in Figure 1b with a homogeneous surface morphology derived for each experiment. This plate was immersed in a mixture comprising 9.2 mL of 30 wt% H 2 O 2 solution and 0.5 g of 85 wt% H 3 PO 4 solution and left to react for 24 h at 120°C. Depending on the synthesis conditions (e.g., the raw material ratio or reaction temperature), this reaction generates high pressure inside the reaction container due to the generated gas, particularly when the hydrothermal reaction takes place at 100°C or higher. A reactor vessel that can measure the internal gas pressure while a reaction occurs inside and that is equipped with a safety valve to release the gas when required is desired. The obtained product was repeatedly washed with distilled water after the reaction. Because titanium phosphate particles that were generated in the solution during reaction deposited on the upper surface (front face), it was not possible to accurately evaluate the morphology of the titanium phosphate thin film growing from the titanium plate. Thus, a detailed evaluation was performed on the lower surface (back face) on which particles did not deposit. A thin film formed evenly on the surface of the titanium plate, as shown in Figure 2a. The thin film is composed of nanobelts with approximate widths of 30-270 nm and lengths of a few microns or more formed on the surface of the titanium plate, as shown in Figure 2b and c. The TEM image (Figure 2c) confirmed that the particles constituting the thin film were nanobelts because they were thin one-dimensional in morphology. Diffraction peaks that were attributed to titanium metal and Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O were observed in the X-ray diffraction measurements (Figure 3). They revealed that the nanobelts were made of Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O. Korösi et al. [25] reported on rectangular Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O nanoparticles with dimensions of 15 ± 3 nm × 20-150 nm. The nanobelts observed during this experiment, however, had nanoparticles with significant growth in the long axis direction. The thin film thickness was about 8 µm at the reaction time of 24 h but this thin film thickness became broader and reached 20 µm when the reaction time was extended to 72 h. SEM image of the thin film cross-section at the reaction time of 72 h are shown in Figure 2d. A phase comprising nanobelts was observed from the top surface of the thin film down to a depth of about 18 µm. On the side closer to the titanium plate of the thin film, however, a phase of about 2 µm that was relatively dense was observed.

Recent Development of Nanofibers
The role of hydrogen peroxide was examined in order to consider the formation mechanism of nanobelts. This is because past studies that did not involve the use of hydrogen peroxide did not generate Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O, as described earlier [28,29]. In order to investigate the impact of hydrogen peroxide on the formation of the thin film, synthesis was performed with a fixed reaction temperature, reaction time, and phosphoric acid quantity of 120°C, 24 h, and 0.5 g, respectively, with varying quantities of hydrogen peroxide and water. When 9.2 mL of water was used for synthesis instead of hydrogen peroxide, the thin film did not form, and the formation of TiH 2 was observed in the XRD measurement. This confirmed that hydrogen peroxide plays an important role in the formation of the Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O nanobelt thin film. Figure 4 shows a schematic representation of the formation mechanism for nanostructured titanium phosphate thin films based on the above results. Hydrogen peroxide has two primary roles. First, it acts as an oxidizer. Hydrogen peroxide acts as an oxidizer in acid solutions according to the following reaction formula: Therefore, hydrogen peroxide promotes the generation of Ti 4+ from the titanium plate, as described by the following equation: ) are known to be generated when pH >1 [31]. These are referred to as peroxotitanic acid complexes and are indicated by a yellow color in the aqueous solution. The color of the solution after synthesis of the titanium phosphate nanobelt thin film was actually yellow, as shown in Figure 5, which indicates that a peroxotitanic acid complex was formed. Therefore, the second role of hydrogen peroxide is the formation of chemical species that contain Ti-O bonds. When such chemical species react with H 2 PO 4 -ions, a relatively dense phase is formed first as an amorphous or low-crystalline titanium phosphate phase precipitates on the titanium plate. The nanobelt thin film forms next as high-crystalline Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O, which is the most stable phase, grows on the surface of the dense phase. The formation of such a dense amorphous phase is important to link the titanium metal with Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O, which has a completely different crystal structure. The phenomenon of a peculiar nanostructured thin film forming via the formation of a relatively dense phase on a metal surface has also been observed for the formation of sodium titanate thin films on titanium plate surfaces [9], which is described later. Synthesis using a mixture comprising 2.3 mL of hydrogen peroxide and 6.9 mL of water instead of 9.2 mL of hydrogen peroxide led to a flower-like aggregate of semispherical morphologies having a diameter of about a few tens of microns while the composition remained the same as Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O. This means that a thin film comprising microflowers was generated (Figure 6a). Observation of the cross-section revealed that the thickness of the thin film was about 30 µm, and the microflower comprised particles with a nanoplate morphology about 400-700 nm wide and about 30-40 µm long growing in a radial pattern. A thin film of Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O having an intermediate morphology between the nanobelt thin film (Figure 2b) and microflower thin film (Figure 6a) was formed by using a mixture comprising 4.6 mL of hydrogen peroxide and 4.6 mL of water instead of 9.2 mL of hydrogen peroxide, as shown in Figure 6b. These results indicate the following formation process. The number of nuclei of Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O formed on the titanium plate decreased significantly once the amount of hydrogen peroxide in the reaction solution decreased. The microflower thin film was believed to have been formed as a large number of Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O crystals slowly grew radially from these nuclei, which were just a few in number. Therefore, the morphology of the thin film can clearly be controlled in a variety of ways while the composition of Ti 2 O 3 (H 2 PO 4 ) 2 ·2H 2 O is maintained by controlling the synthesis conditions as described above.  Thin films with compositions other than Ti 2 O 3 (H 2 PO 4 ) 2 2H 2 O or of various morphologies other than nanobelts can be generated by controlling the raw material ratios and reaction temperatures, although no detailed descriptions are provided here because this chapter is focused on nanobelts. For instance, a thin film composed of anatase-type titanium dioxide and about 2 µm thick, a few tens of nanometers wide, and about 2 µm long-similar to that reported by Wu et al. [32]-was generated when synthesis was performed with a reaction temperature of 120°C, 9.  The wettability of these thin films was investigated since titanium phosphate thin films having a diverse range of surface morphologies were derived, as described above. The wettability of a surface is generally determined by the surface energy and uneven structure of the surface [33]. In cases where the surface energy is high, the thin film tends to be hydrophilic; when the surface energy is low, it tends to be hydrophobic. Furthermore, the wettability is enhanced when an uneven structure is formed on a surface. When a water droplet was dropped on a synthesized titanium phosphate thin film, the droplet spread instantaneously, which indicates super-hydrophilicity. When the thin film was immersed in a 0.1 mol/L n-dodecyl decylamine hydrochloride solution of 2-propanol, on the other hand, the hydrophilicity decreased because the surface energy decreases when dodecylamine is adsorbed on the surface of titanium phosphate. The contact angle was 25° with the nanobelt thin film, 98° with the microflower thin film, 151° with the nanorod thin film, 151° with the nanoplate thin film, and 128° with the nanosheet thin film. Thus, the contact angle varies significantly depending on the composition and morphology of the thin film. Common features of test specimens with larger contact angles (nanorod thin film, nanoplate thin film and nanosheet thin film) were that the gap between protruding parts was narrow, and the spacing was uniform. With the nanobelt thin film, on the other hand, there were portions where the spacing between protruding parts was narrow and others where they were wide since the nanobelts were bent. The wider sections in such instances decreased the contact angle. Furthermore, the contact angle may have been reduced because there were portions on the microflower thin film where titanium phosphate microflowers were not generated and water was absorbed.

Synthesis and antibacterial activity of titanate nanofiber thin films
A titanium plate (20 mm × 20 mm × 2 mm) was immersed in 20 mL of a NaOH aqueous solution in a Teflon container; a hydrothermal reaction occurred at 160°C for 20 h. The derived product was then washed repeatedly with water to produce a titanium plate covered by a sodium titanate nanofiber thin film on the surface, i.e., a sodium titanate nanofiber thin film/titanium metal composite. A digital camera photograph and a SEM image (Figure 8a) confirmed that nanofibers were formed uniformly on the surface of the titanium metal. SEM observation of the cross-section revealed that the thin film thickness was about 5 µm. Furthermore, the TEM images revealed that the nanofibers were a few tens to a hundred nanometers in width and a few microns in length (Figure 8b). The diffraction peaks attributed to titanate were observed in the X-ray diffraction pattern of the thin film. A Na:Ti:O molar ratio of 1:3.04:6.08 was determined from the EDS analysis of the thin film. This approximates the molar ratio for Na 2 Ti 6 O 13 . Thus, the thin film was considered to be a Na 2 Ti 6 O 13 nanofiber thin film. The morphology of the sodium titanate thin film can be controlled according to the concentration and reaction temperature of the aqueous solution of NaOH. Using 1 mol/L NaOH aqueous solution instead of 4 mol/L NaOH aqueous solution resulted in the generation of a nanosheet thin film (Figure 9a) [10]. Using 10 mol/L NaOH aqueous solution resulted in the generation of a nanotube thin film (Figure 9b) [9]. Using 5 mol/L NaOH aqueous solution for a reaction at 60°C for 24 h resulted in the generation of a thin film with a porous network structure [5,34].   [34], and the sodium titanate thin film with a porous network structure (c) [34] formed on titanium plates.
The antibacterial activity of sodium titanate nanofiber thin film against methicillin-resistant staphylococcus aureus (MRSA) was investigated. MRSA is one of principal causative agents for nosocomial infection. Two types of tests were performed for the antibacterial test: the test specimen was irradiated and not irradiated with ultraviolet rays with the expectation of photocatalytic activity. Refer to the authors' previous paper for details on the methods used in the antibacterial tests [34]. The antibacterial activity value was used as an index for evaluation. The antibacterial activity value R is defined by the following equation; the antibacterial activity is considered to exist when R ≥ 2.
Here, A is the viable bacterial count immediately after inoculation, B is the viable bacterial count of a blank test specimen 24 h later, and C is the viable bacterial count of the nanostructured titanate thin film 24 h later.
A high antibacterial activity value of 5.9 was recorded during the first round of antibacterial tests conducted under exposure to ultraviolet irradiation. Surprisingly, an extremely high antibacterial activity value of 5.8 was recorded even when a similar antibacterial test was conducted with no exposure to ultraviolet rays. This means that a high level of antibacterial activity was recorded for the nanofiber thin film during the first round of antibacterial tests regardless of whether or not the test specimen was exposed to ultraviolet rays. The antibacterial test was repeatedly performed using the same test specimen with and without (i.e., in a dark place) exposure to ultraviolet rays. Extremely high values of 7.7 and 6.6 were recorded for antibacterial activity during the second and third rounds of tests conducted under exposure to ultraviolet rays. Extremely low values of 1.0 and 0.3 were recorded for antibacterial activity during the second and third rounds of antibacterial tests conducted without exposure to ultraviolet rays. The above results revealed that (1) a high level of antibacterial activity manifested with the sodium titanate nanofiber thin film due to photocatalysis effects with exposure to ultraviolet rays and that (2) the sodium titanate nanofiber thin film has a high level of antibacterial activity immediately after synthesis, even when they are not exposed to any ultraviolet rays. Only the sodium titanate nanofiber thin film indicating a high level of antibacterial activity is extremely peculiar because the antibacterial activity of the sodium titanate nanosheet thin film and nanotube thin film described above were 2.1 [10] and 1.7 [34], respectively. Treating such sodium titanate nanofiber thin film with a hydrochloric acid aqueous solution or heat-treating it at 600°C reduced the antibacterial activity value to 0.9 and 1.3, respectively, which eliminated the antibacterial activity. Because bacteria are known to have different properties that classify them as gram-positive or gram-negative depending on their structures, antibacterial tests regarding such individual bacteria types were also conducted. The antibacterial activity value of the sodium titanate nanofiber thin film was 5.5 against Escherichia coli, which is a gram-positive bacteria, and 4.7 against Staphylococcus aureus, which is a gram-negative bacteria. This reveals that sodium titanate nanofibers have a high level of antibacterial activity against bacteria of both structures. Because the reaction time was extended from 1 to 20 h, the formation of nanofibers was clarified according to the SEM images depicted in Figure 10. The intensities of the respective diffraction peaks were greater in the X-ray diffraction pattern, as shown in Figure 11. The antibacterial activity values of the thin films generated with reaction times of 1 and 5 h were 1.2 and 0.3, respectively, which indicates no antibacterial activity. The antibacterial activity value for the thin film generated with a reaction time of 12 h was 3.1; thus, the antibacterial activity manifested. The value was 6.5 for the thin film generated with a reaction time of 20 h; thus, a high level of antibacterial activity occurred. The above results indicate that the antibacterial activity increases with the growth of nanofibers and enhanced crystallinity. While it is not possible to offer any clear reason for such high levels of antibacterial activity at present, the following assumptions were made. All nanostructure morphologies (i.e., nanofiber, nanosheet, and nanotube) involve layering and rolling of titanate sheets, and the surface and the edge sections of titanate sheets can be considered separately because of their different chemical properties [35]. Nanofiber structures are characterized by having the highest proportion of the edge section to the overall area compared with other nanostructures. This may mean that the edges of titanate sheets become active sites and affect bacteria in some way, which manifests as a high level of antibacterial activity.  Sodium titanate nanofiber thin film is considered to have poor practicality at the current point in time; while superior antibacterial activity was indicated during the first round of tests, the results from the second and subsequent rounds were not good, and there are many unknown aspects about the antibacterial mechanism. Silver, which is already being used in many studies on antibacterial materials, was therefore considered as an antibacterial component based on three considerations: (1) the superior antibacterial activity, (2) some degree of safety for living organisms, and (3) the relative lack of reports regarding the manifestation of drug-resistant bacteria [36]. Sodium titanate is a cation exchanger with a layered structure and can support silver in the titanate by exchanging Na + in sodium titanate with Ag + . A composite treated with silver ion exchange was derived by immersing the sodium titanate nanofiber thin film/titanium metal composite generated with a reaction time of 20 h in 12 mL of a 0.05 mol/L silver acetate aqueous solution at 40°C for 3 h, which was then washed repeatedly with distilled water and dried in a cool and dark location. The thin film derived after the silver ion exchange process remained firmly attached to the titanium plate, as shown in the digital camera photographs and SEM images in Figure 8c. There were no changes in the morphology, which had dimensions on the scale of microns. The TEM images, however, revealed precipitation of nanoparticles with dimensions of a few nanometers to a few tens of nanometers on the surface of the nanofiber (Figure 8d). These particles were considered to be silver nanoparticles, but no diffraction peaks attributed to silver were observed in the X-ray diffraction pattern (Figure 12). This may have been due to the small amount of precipitation that occurred because of the small diameters of the silver nanoparticles. No sodium was detected from the thin film after the silver ion exchange process, but silver was detected when the EDS analysis was conducted. A silver-to-titanium molar ratio of 0.638 was detected; this is greater than the sodium-to-titanium molar ratio of 0.329 before the silver ion exchange process. Such results of the EDS analysis correspond to the precipitation of silver particles on the surface of nanofiber when Na + in the sodium titanate nanofiber was exchanged with Ag + . The X-ray diffraction pattern after the silver ion exchange process indicates the disappearance of the diffraction peak of 2θ = 8.78°, which was caused by the layered structure observed with sodium titanate (Figure 12). Significant changes in the position and strength of the diffraction peaks at 2θ ≥ 15°owing to the crystal structure of titanate were observed compared to before the silver ion exchange process (Figure 12). Such results indicate that the crystal structure of titanate changed from a two-dimensional layered structure to a three-dimensional crystal structure. The above results reveal that silver nanoparticles and silver titanate nanofiber thin film are generated by the silver ion exchange process of the sodium titanate nanofiber thin film. Such precipitation of silver nanoparticles on the surface of titanate and change in crystal structure caused by the silver ion exchange process have also been observed with the sodium titanate nanotube thin film and the sodium titanate thin film with a porous network structure [34]. This suggests that these are reaction behaviors common to layered titanates. The antibacterial activity value of the silver nanoparticles/silver titanate nanofiber thin film with respect to MRSA is 7.9, which is extremely high. This value is greater than the antibacterial activity value of 6.3 for a silver nanoparticles/silver titanate nanotube thin film or 7.8 for a silver nanoparticles/silver titanate thin film with a porous network structure [34]. The elution behavior of silver was investigated in fetal bovine serum (FBS) to simulate the environment of a living body because it was presumed to impact the antibacterial activity in a significant manner. Please refer to the authors' previous paper for details on the experimentation methods. Elution of 110 ppm was observed with silver nanoparticles/silver titanate nanofiber thin film, 94 ppm was observed with the silver nanoparticles/silver titanate nanotube thin film, and 68 ppm was observed with the silver particles/silver titanate thin film with a porous network structure on the first day. The greatest amount of silver was clearly eluted by silver nanoparticles/silver titanate nanofiber thin film with the highest level of antibacterial activity. The orders of antibacterial activity and elution quantity for silver did not match completely. Therefore, antibacterial activity cannot be explained solely by the elution amount of silver.
Although a clear examination is not possible at the present point in time, the surface morphology of the thin film is considered to be somehow related to the antibacterial activity. Chronologically, the amount of silver elution was 110 ppm on the first day, decreased to 77 ppm by the second day, and gradually decreased with subsequent days to 20 ppm by the tenth day. The elution of silver from silver nanoparticles and from silver titanate nanofiber due to the ion exchange reaction were considered as sources of silver elution from the thin film. FBS is known to contain a large amount of proteins and cations that readily bond with Ag + , and the ion exchange reaction is known to be extremely rapid. This means that it is extremely likely that the large amount of silver elution on the first day was due to the silver elution from the ion exchange reaction of silver titanate nanofibers. The contribution to silver elution by the silver titanate nanofibers decreased with time; instead, the silver elution from silver nanoparticles increased in their place. The antibacterial activity can be expected to be sustained over a long period since the silver nanoparticles/silver titanate nanofiber thin film can continue to elute silver in this manner for over 10 days.

Conclusion
This chapter introduces the generation of titanium compounds with various nanostructures, including titanium phosphate nanobelts and titanate nanofibers on the surface of titanium metal plate, from the treatment of a titanium metal plate in aqueous solutions of various compositions. Because aqueous solutions of various compositions and concentrations and different reaction temperatures and times can be combined for chemical processes involving titanium metal, novel titanium compounds/titanium metal composites with various compositions and nanostructures are expected to be synthesized in the future. It is important to continue with applied research on developing composites that feature the characteristics of both nanostructured titanium compounds and titanium metal, as described in Section "Introduction." In this manner, characteristics that surpass those of the nanostructured titanium compound particles can be derived. Research is expected to continue with applications including biomaterials and electrode materials intended for dye-sensitized solar cells, lithium-ion batteries, and sodium-ion batteries.