Anode Biofilm

Results of quantitative RT-PCR of gene transcript levels during biofilm growth were consistent with microarray results. OmcZ and the outer-surface C-type cytochrome OmcE were more abundant and OmcS was less abundant in current-harvesting cells. The role of outer-surface proteins whose genes were expressed in the current-producing biofilm versus the control biofilm which reduced fumarate were evaluated by gene deletion and its impact on current production was examined. It was found that deletion of OmcS, OmcB, or OmcE had no impact on maximum current production. Deletion of OmcZ significantly reduced power production. These results suggest that OmcZ is a key component in electron transfer in MFCs with a G. sulfurreducens anode In thin (ca. 10 mm) wild type biofilms, genes for OmcS and OmcE are more highly expressed than in planktonic cells grown with a soluble electron acceptor such as Fe(III) citrate CymA, which participates in many Shewanella anaerobic respiration processes, is a tetraheme C-cytochrome whose C-terminal is exposed to the periplasm and its N-terminal is anchored in the inner membrane. In the case of electrode reduction, a deletion of the CymA gene caused >80% decrease in current generation. CymA can interact directly with many terminal reductases in the periplasm, such as fumarate electrochemically oxidized the surface of a graphite felt electrode with a strong acid in order to stimulate current production in the MFC. FT-IR was used to examine the chemical property changes in the graphite felt surfaces which were induced by its electro‐ chemical oxidation using acid treatment. Current production in the MFC equipped with the surface-modified graphite anode was about 40% higher than that obtained from the MFC of a bare graphite anode. FT-IR spectra of surface-modified felt and bare graphite felt showed a notable broad band at 3264 cm -1 ascribed to stretching vibration of OH within –COOH, a broad peak at 1560cm -1 ascribed to C=O of ketone and carboxyl groups, and a peak at 1419 cm -1 ascribed to bending vibration of –OH. The relative intensities of the abovementioned peaks increased significantly after electrochemical oxidation treatment of graphite felt, indicating that alcohol and carboxyl functional groups formed on the surface of the graphite-felt hydrogen bonding with peptide bonds in the bacterial cytochrome. They also demonstrated that the carboxylic acid terminus of gold-modified electrodes can facilitate the binding with cytochrome on its surface, and as a result, current production increased due to the enhanced transfer of electrons from the interior of the cell [49]. In vitro and in vivo experiments led to the proposal of several models based on the redox properties of bacterial C-cytochromes. A 4-step mechanism for electron transfer in Geobacter biofilms was proposed: step 1, acetate uptake and electron


Introduction
Microbial fuel cells (MFCs) have long been considered an attractive mean for converting various carbohydrate wastes directly into electricity using electrogenic bacterial cells in the anode compartment. Most MFCs have been operated using anaerobic or facultative aerobic bacteria which oxidize various substrates including glucose, sewage sludge and petroleum hydrocarbon [1]. Power production by MFCs varies with bacterial cell species, specific substrate concentration, cathode catalysts and the MFC configuration [2].
Typically, MFCs which were operated with a mixture of bacterial cells produced higher specific power than MFCs operated by a monoculture in the anode compartment [1]. Knowledge and understanding of the anode biofilm components, morphology formation steps and electron transfer mechanism may lead to better biofilm conductivity in MFC.
This chapter focused on biofilm definition and composition. Spectroscopic methods for anode biofilm study, including advancing real-time analysis. Power-producing bacterial cells, mechanisms of electron transfer from the biofilm to the anode and the effect of medium and pH conditions on power production.

Biofilm definition
The term biofilm has been proposed for a structured community of microorganisms that adheres irreversibly to surfaces (biotic or abiotic) and is enclosed in a self-developed polymeric matrix of a primarily polysaccharide material [3].
The biofilm is enriched with specific protein adhesins that mediate known molecular binding mechanisms for irreversible attachment. In addition, membrane transport proteins such as porins and extracellular enzymes are up-regulated [15]. Biofilm formation occurs in a sequential process of: (i) transport of microbes to a surface by chemotaxis or Brownian motion; (ii) initial attachment; (iii) irreversible attachment of bacteria and formation of microcolonies; (iv) biofilm maturation; and (v) detachment [16].
The substratum characteristics may influence biofilm formation and morphology. Most investigators have found that microorganisms attach more rapidly to hydrophobic, nonpolar surfaces such as teflon and other plastics than to hydrophilic materials such as glass or metals [17]. Furthermore, microbial colonization increases with the increase in surface roughness [18]. The biofilm architecture changes constantly, due to external and internal processes [16]. The biofilm thickness may be affected by the number and species of microorganisms. Biofilms of pure cultures of either Klebsiella. pneumoniae or P. aeruginosa in a laboratory reactor were thinner (15 μ and 30 μ, respectively), whereas a biofilm containing both species was thicker (40 μ) [19]. Mature bacterial biofilms can adopt various architectures depending on the characteristics of the surrounding environment, such as nutrients, pH, temperature, shear forces, osmolarity and composition of the microbial consortia. The common complex biofilm is a mushroom-like structure which is surrounded by highly permeable water channels that facilitate the transport of nutrients and oxygen to the interior of the biofilm [8].
Cell surface-associated proteins such as pili, flagella, curli and amyloid fibers are believed to be important factors for biofilm formation [20]. Cell-to-cell signaling (quorum sensing) has been demonstrated to play a role in biofilm formation. P. aeruginosa produces two different quorum sensing molecules, lasR-lasI and rhlR-rhlI, which were shown to be involved in biofilm formation [21]. The biofilm of a double mutant produced thinner biofilms than the wild type, its cells were more densely packed, and the typical biofilm architecture was absent. Addition of the quorum sensing molecules known as homoserine lactone to a medium that contained the mutant biofilms resulted in biofilms with a structure and thickness similar to that of the wild type [17,22].
In conclusion, knowledge and understanding of the biofilm components, morphology and formation steps may lead to better biofilm conductivity in microbial fuel cells (MFC).

Spectroscopic methods for anode biofilm study, including advancing real-time analysis
During the last decade, most efforts in microbial fuel cell (MFC) research focused on modifications of MFC design and electrode materials, with little investigation of the properties of the anode microorganisms that are essential for maximal current production. However, understanding the functions of microbial cell surfaces requires knowledge of their chemical structural, physical properties and biological processes.
Perhaps the most challenging effort to improve MFC power production lay in the fundamental understanding of the biofilm's chemical, physical and biological characteristics. Study of the local properties of an anode biofilm is an even a greater challenge, due to the low concentration of bacterial cells. In addition to conventional methods such as scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), there exist modern methods such as Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). These latter are nondestructive methods which can probe biofilms down to single-cell surfaces with high resolution, and thereby promote better understanding of biofilm formation, functionality and activity.
In this chapter we will discuss the principle, the advantages and the disadvantages of each of the methods and the application of these techniques in MFCs.

Confocal laser scanning microscopy
Confocal laser scanning microscopy (CLSM) is often used in optical imaging of sliced microfluidic velocity fields by mapping the investigated focal plane. CLSM enables obtaining a series of optical sections of intact undisturbed biological samples as thin as 0.3 μm. Commonly used analyses that rely on staining techniques are applied to determine the architecture, spatial distribution and viability profile in microbial biofilms. The most popular application of CLSM is for identification of live and dead bacteria. Simultaneous measurements of anodic biofilms during the MFC's operation may be obscured by the need to apply labeling materials. This method is therefore used more commonly as a useful ex situ method [23,24].

Scanning electron microscopy
Scanning electron microscopy (SEM) analysis provides an excellent magnification technique that uses a condensed electron beam to scan a sampled object and magnify specific regions of its surface area. Highly resolved images can be produced by SEM to provide morphological details of the surface, information on the three-dimensional topography as well as an elemental composition analysis map of the same unit area when coupled with an x-ray elemental detection sensor. SEM is therefore instrumental in a broad spectrum of scientific and industrial applications.
The main shortcomings of SEM are the relatively low resolution compared to transmission electron microscopy (TEM), which is usually higher than a few tens of nanometers and the need for a surface-conducting coating layer to avoid local charging and heating effects. Moreover, SEM is an ultra-low pressure technique. All samples must therefore withstand the low pressure inside the SEM vacuum chamber [25].

Raman spectroscopy
The Raman spectroscopy method is based on inelastic scattering of monochromatic photons, usually from a laser source. In this process, the frequency of the back-scattered photons changes their frequency due to interaction with a sample. The process begins when photons of the laser light are absorbed by the sample and are then re-emitted at a longer wavelength compared with the laser's original monochromatic frequency. This phenomenon is called the Raman frequency shift effect. This shift provides information on vibrational, rotational and other low frequency transitions at the molecular level. Raman spectroscopy is typically applied in the study of solid, liquid and gaseous samples.
The main strength of the Raman technique is its high sensitivity, the fact that it does not require any staining and its ability to generate detailed chemical and spatial information with a resolution below the diffraction limit. A combination of Raman spectroscopy with optical microscopy provides a powerful source of information which is both detailed and sensitive and can be obtained with a spatial resolution below the diffraction limit [26,27]. The main disadvantages of the Raman technique is the weak Raman effect, which requires sensitive and highly optimized instrumentation for detection. Furthermore, fluorescence of the sample or impurities within it can mask the Raman spectrum. Sample heating through the intense laser radiation can damage the sample and distort the Raman spectrum [25].

Atomic force microscopy
Atomic force microscopy (AFM) provides one of the highest topographical profile imaging of a sampled surface, sometimes even on a nanometric scale. It does so by measuring interaction or repulsion forces between a sharp nano-size probe and a surface. The nitration distances between the tip and the sample can be as small as a few tenths of a nanometer. The AFM tip can be used either in a contact mode where the tip actually touches the surface, or in a noncontact mode where van der Waals interactions produce forces across the short distance between the tip and the surface. The forces produced by each mode of operation can be recorded to give topographic, conductive, magnetic, mechanical (friction forces) or potential images of the surface.
In fact, AFM imaging does not require any surface modification that may damage or change the sample. Most importantly, AFM techniques are well suited to work in ambient air or in a liquid environment, including physiological conditions [25]. Therefore, the study of electrodes, biological samples, biomolecules and even living organisms can be measured rather easily by AFM. Moreover, advanced surface-imaging tools offer more than just force measurements. It can be applied to probe physical properties such as molecular interactions, surface hydrophobicity, surface charges, mechanical properties and local electrochemical properties [28]. For example, AFM enables imagining of biofilms and the EPS distribution within the biofilm [29].
The disadvantage of AFM is that the scanned area of the AFM image is rather small (few tens of microns), and it is very sensitive to externally generated electrical and mechanical noises [25].

Power-producing bacterial cells
Pure cultures are suitable for basic study of MFCs, since they allow analysis of electrochemical and biochemical processes as well as high reproducibility. However, mixed cultures are more suitable for industrial applications because no sterilization is required and it is not necessary to maintain anaerobic conditions via an external N 2 flow. Mixed bacteria usually produce higher power densities in MFCs than pure bacterial strains. It is important to note that the power density depends on the specific MFC configuration, electrode spacing, bacterial cell species, growth medium and the cathode catalysts [1,2]. Thus, power densities produced in a MFC using a specific bacterium cannot be directly compared with another bacterium unless all other parameters are identical.
Exoelectrogenic bacteria transfer electrons to anodes either directly or via self-produced mediators. In the direct mechanism, electron transfer occurs via membrane-associated Ccytochrome or through conductive pili or appendages [33]. In mediated mechanisms, electron transfer between the bacterial cell and the anode surface occurs through self-produced soluble redox compounds such as flavins or pyocyanin [34]. Well-known exoelectrogens include Geobacter sp. and Shewanella sp. [35] which are known to have outer-membrane cytochromes and conductive pili, and Pseudomonas sp. that produce mediators that shuttle electrons from the bacteria through the biofilm matrix to the anode [36].
6. Mechanisms of electron transfer from the biofilm to the anode 6.1. C-cytochrome C-type cytochromes have been considered as one of the most important electron transfer strategies in current generation by exoelectrogens. C-cytochromes are heme-containing proteins which are widespread in most bacteria. S. oneidensis MR-1 has 42 putative C-cytochromes and 80% of them are located in the outer membrane, covering 8-34% of the cell surface. It was reported that in Geobacter, C-cytochromes are bound to the matrices of extracellular polymeric substrates [37]. Appendages termed bacterial nanowires that were identified in both Shewanella and Geobacter also contain surface-located C-cytochromes which are postulated to transport electrons from distant cells to electrodes [38,39]. However, these studies are based on in vitro experiments and more research needs to be done to understand this mechanism [40]. Geobacter biofilm respiration was found to continue after the interruption of electrode polarization, since these bacteria can store electrons in the heme of the exocytoplasmic cytochromes. When the electrode was connected again, stored electrons were recovered as a current superimposed on the basal steady-state current [41].
Cytochrome genes were examined during extracellular electron transport in MEC. A total of 21 cytochrome genes were detected. Four bacterial genera contain the cytochrome genes: Geobacter, Desulfovibrio, Rhodopseudomonas and Shewanella, all of which increased over three months of the MEC reactor's performance [32]. Microarray analysis of Geobacter sulfurreducens thick anode biofilms (>50 μm) in MFCs revealed 13 genes in current-harvesting biofilms. Up-regulated genes included two outer C-type membrane cytochromes, OmcB and OmcZ. Down-regulated genes included the genes for the outer-membrane C-cytochromes OmcS and OmcT. Results of quantitative RT-PCR of gene transcript levels during biofilm growth were consistent with microarray results. OmcZ and the outer-surface C-type cytochrome OmcE were more abundant and OmcS was less abundant in current-harvesting cells. The role of outer-surface proteins whose genes were expressed in the current-producing biofilm versus the control biofilm which reduced fumarate were evaluated by gene deletion and its impact on current production was examined. It was found that deletion of OmcS, OmcB, or OmcE had no impact on maximum current production. Deletion of OmcZ significantly reduced power production. These results suggest that OmcZ is a key component in electron transfer in MFCs with a G. sulfurreducens anode [42]. In thin (ca. 10 mm) wild type biofilms, genes for OmcS and OmcE are more highly expressed than in planktonic cells grown with a soluble electron acceptor such as Fe(III) citrate [43]. CymA, which participates in many Shewanella anaerobic respiration processes, is a tetraheme C-cytochrome whose C-terminal is exposed to the periplasm and its N-terminal is anchored in the inner membrane. In the case of electrode reduction, a deletion of the CymA gene caused >80% decrease in current generation. CymA can interact directly with many terminal reductases in the periplasm, such as fumarate reductase and nitrate reductase. Bacterial two-hybrid showed pair-wise interactions among CymA, MtrA and some other periplasmic redox proteins, indicating that CymA is the major electron conduit to the periplasmic space and can interact directly with periplasmic redox proteins by forming a transient protein complex [44].
Okamoto et al identified voltammetry signals of outer membrane C-cytochrome in monolayer biofilms of S. oneidensis [45]. Okamoto et al analyzed monolayer and multilayer biofilms of S. oneidensis on tin-doped indium oxide electrodes and compared the respective amounts of electroactive C-cytochrome using voltammetry techniques. The scan-rate dependence of cyclic voltammograms was used to investigate the role of C-cytochrome in the transfer of respiratory electrons to distant tin-doped indium oxide electrodes. Electron conduction in S. oneidensis MR-1 multilayer biofilms was demonstrated to be mediated by sequential redox cycling of outer membrane C-cytochrome under normal physiological conditions. It was also demonstrated that the electron transport outer membrane C-cytochrome conduit across the biofilms contributed to the anodic current generation [40].
Immunogold labeling of the outer-surface C-type cytochrome OmcZ showed that when G. sulfurreducens grew as a biofilm on a graphite electrode that served as an anode and sole electron acceptor for growth, OmcZ was highly concentrated at the biofilm-electrode interface. Control biofilms which were grown on the same graphite material but with fumarate as the electron acceptor did not have OmcZ accumulations at the biofilm-electrode interface. The researchers suggested that OmcZ may serve as an electrochemical gate facilitating electron transfer from G. sulfurreducens biofilms to the anode surface [46]. Direct current surfaceenhanced infrared (IR) absorption spectroscopy and FTIR demonstrated a linear correlation between the increasing presence of Geobacter sulfurreduces protein and current production. This result may confirm that the extracellular cytochromes [47] are responsible for the electron transfer to the gold electrode [48].
Eaktasang et al. electrochemically oxidized the surface of a graphite felt electrode with a strong acid in order to stimulate current production in the MFC. FT-IR was used to examine the chemical property changes in the graphite felt surfaces which were induced by its electrochemical oxidation using acid treatment. Current production in the MFC equipped with the surface-modified graphite anode was about 40% higher than that obtained from the MFC of a bare graphite anode. FT-IR spectra of surface-modified felt and bare graphite felt showed a notable broad band at 3264 cm -1 ascribed to stretching vibration of OH within -COOH, a broad peak at 1560cm -1 ascribed to C=O of ketone and carboxyl groups, and a peak at 1419 cm -1 ascribed to bending vibration of -OH. The relative intensities of the abovementioned peaks increased significantly after electrochemical oxidation treatment of graphite felt, indicating that alcohol and carboxyl functional groups formed on the surface of the graphite-felt hydrogen bonding with peptide bonds in the bacterial cytochrome. They also demonstrated that the carboxylic acid terminus of gold-modified electrodes can facilitate the binding with cytochrome on its surface, and as a result, current production increased due to the enhanced transfer of electrons from the interior of the cell [49]. In vitro and in vivo experiments led to the proposal of several models based on the redox properties of bacterial C-cytochromes. A 4-step mechanism for electron transfer in Geobacter biofilms was proposed: step 1, acetate uptake and electron transfer to periplasmic cytochromes; step 2, subsequent electron transfer to the exocytoplasmic cytochromes; step 3, electron transport along the biofilm matrix cytochromes; and step 4, transfer between the interfacial cytochromes and the electrode [41].

Pili
Genes encoding proteins with a PilZ domain were deleted from the G. sulfurreducens genome in an attempt to study the importance of pili to biofilm conductivity. The mutant strain designated as strain CL-1 produced more pili than the wild type strain and formed 6-fold more conductive biofilms than the wild type strain. Heme-staining revealed a higher abundance of cytochrome with a molecular weight consistent with OmcS in CL-1 and Western blot analysis with OmcS-specific antiserum confirmed higher production of OmcS in CL-1. Immunogold labeling coupled with TEM demonstrated that OmcS was localized on the pili of CL-1 [50]. Multilayer biofilms of Geobacter sulfurreducens on the anode surface of a MFC remained viable even at a distance from the anode. There was no decrease in the efficiency of current production with an increase in the thickness of the biofilm. Genetic studies demonstrated that efficient electron transfer through the biofilm required the presence of electrically conductive pili which represent an electronic network promoting long-range electrical transfer in an energy-efficient manner in the MFC [38].
G. sulfurreducens KN400 bacterial cells which were selected after 5 months of incubation at-400 mV produced higher current and power densities than the original inoculum (strain DL1). The enhanced capacity for current production in KN400 was associated with a greater abundance of electrically conductive microbial nanowires than DL1 and lower internal resistance in KN400 fuel cells. KN400 produced flagella, whereas DL1 does not. The changes in outer surface components were associated with a greater propensity of strain KN400 to stick to surfaces than strain DL1. KN400 cells grown with fumarate as the electron acceptor were clumpy and strongly adhered to the glass surface of the culture tubes. This research showed that microorganisms' ability to electrochemically interact with electrodes can be enhanced with the appropriate selective pressure and that improved current production is associated with clear differences in the properties of the outer surface of the cell that may provide better microbeelectrode interactions [51].
The AFM technique, together with electrochemical measurements by scanning tunneling microscopy (STM), allowed mapping of conducting substrates and microbial pili in MFCs studies. These two techniques enabled identification of the pili on Geobacter species. The existence of such pili raises the possibility of long-range direct electron transfer from each microbe to the electrode [36,48]. El-Naggar et al. evaluated transport along the bacterial nanowires by conducting probe AFM at several points alongside a single nanowire using a metallic electrode and a conductive AFM tip. S. oneidensis MR-1 nanowires were electrically conductive along micrometer-length scales, yielding a corresponding electron transport rate, at 100 mV, of about 109 electrons per second, with an estimated resistance of 1 Ω (Fig 1). It was also found that mutants deficient in genes for the C-type decaheme-cytochromes MtrC and OmcA produced supplements that are morphologically consistent with bacterial nanowires, but were found to be nonconductive [52].

The effect of medium and pH conditions on power production
The effects of anodic pH on electricity production in two-chamber MFCs inoculated with anaerobic activated sludge were examined. The maximum power density was 1170±58 mWm 2 with a current density of 0.18 mA×cm -2 at pH 9.0, which was 29% and 89% higher than in MFCs operated at pH 7.0 and 5.0, respectively. Electrochemical measurements demonstrated that the environmental pH may influence the electron transfer kinetics of the anodic biofilms. At pH 9.0, the apparent electron transfer rate constant and exchange current density were greater, whereas charge transfer resistance was smaller than under other pH conditions. SEM analysis reveled better biofilm formation at pH 9.0 compared to pH 7.0 and 5.0. The biofilm at pH 9.0 showed increased electron transfer efficiency with respect to the electrocatalytic current, electron transfer rate, exchange current density, and charge transfer resistances, compared with biofilms at pH 7.0 and 5.0. These results demonstrate that electrochemical interactions between bacteria and electrodes in MFCs are greatly enhanced under alkaline conditions, which can an important reason for the improved current production in MFCs [53]. It should be noted that the cathodic potentials were almost identical under all pH conditions, whereas the anodic potentials varied. The anode that operated at pH 9.0 had the most negative individual potentials in the entire current range, whereas the anode at pH 5.0 had the most positive individual potentials. The variations in the power outputs thus resulted from the anodes under different pH conditions [53].
The performance of two dual-chambered mediators-less MFCs was evaluated at different sludge loading rates and pH environments. A maximum volumetric power of 15.51 W/m 3 and 36.72 W/m 3 was obtained in MFC-1 (feed pH 6.0) and MFC-2 (feed pH 8.0), respectively. These results indicate that higher feed pH (8.0) led to higher power production [54]. Zhuang et al. discovered that an alkaline anode (pH 10) led to higher power production during wastewater treatment compared to a MFC which worked at neutral pH. Their assumption is that there methanogenesis is suppressed at higher pHs, and this contributes to a significant enhancement of coulombic efficiency [55]. In contradistinction to the abovementioned studies, there are several studies which described that higher power production was obtained in natural or acidic environments. An air-cathode MFC which operated with a mixed bacterial culture at different pHs showed that the anodic microbial process preferred a neutral pH and that microbial activities decreased at higher or lower pHs [56]. A MFC with a pure culture of Enterobacter cloacae was evaluated as a function of variations in the pH microenvironment. Operation under pH 6.5 and 7.4 led to maximum current generation of 0.40 mA and 0.42 mA, respectively. However, MFC operation under higher pH environments of 8.5 and 9.5 led to a maximum current generation of only 0.38 mA and 0.27 mA, respectively [57].
In conclusion, we assume that MFC power production is dependent on the pH environment and may correlate with the bacterial cell anode species.

Anode biofilm thickness, morphology, viability and conductivity
CSLM analyses revealed a correlation between current and increasing coverage of the anode surface with cells. The average height of the biofilm pillars at currents nearing the maximum current outputs in batch mode was 40 μm (±6 μm), and some cell clusters were as high as 50 μm. Protein measurements of the biofilm biomass at different points during current production indicated a direct linear increase in the amount of biomass on the anodes as the current increased and the biofilms developed. Viability staining indicated that during biofilm development, cells at a distance from the electrode surface remained viable, metabolically active and involved in electron transfer to the anode [38].
Geobacter can form multi-microbe thick, more than 20-cell length, persistent biofilms when the anode serves as their terminal electron acceptor. This phenomenon was not observed in the presence of insoluble oxidants for respiration. The combination of robust direct electron transfer and high cell surface density enables Geobacter biofilms to achieve higher anodic current densities than any other species [58].
The behavior of MFCs during initial biofilm growth and characterization of anodic biofilm were studied using two-chamber MFCs with activated sludge as inoculum. When the biofilms were well developed, a maximum closed circuit potential of 0.41 V and 0.37 V (1000 Ω resistor) was achieved using acetate and glucose, respectively. SEM analysis revealed rod-shaped cells, 0.2-0.3 mm wide by 1.5-2.5 mm long, in the anode biofilm in the acetate-fed MFC, mainly arranged in a monolayer. The biofilm in the glucose-fed MFC was made of cocci-shaped cells in chains and a thick matrix [60. A MFC was operated with a pure culture of Cupriavidus basilensis bacterial cells grown in a defined medium containing acetate or phenol. Operating this mediator-less MFC under a constant external resistor of 1 kΩ with acetate or phenol led to a current generation of 902 and 310 mA m -2 , respectively. SEM and confocal microscopy analyses demonstrated a developed biofilm with pili/appendages and a live anode C. basilensis population which was stained with a LIVE/DEAD viability kit and further analyzed by CSLM (Fig 3) [31].
Electron transfer between bacterial cells and the electrode is one of the major blockages to a desired power density. A direct electron transfer process between P. aeruginosa bacterial cells and the electrode was investigated using cationic reagents which are known to "perforate" the bacterial membrane. Three reagents, chitosan, ethylenediaminetetraacetic acid (EDTA) and polyethyleneimine (PEI), were explored. The surface morphologies of chemically treated P. aeruginosa cells were examined with SEM and AFM images. The results showed that the chemical treatment caused changes in the cell shape and perforated bacterial cells, contrary to the original P. aeruginosa cells that were plump and smooth. The cells were flattened with pores and clusters on the surface. The chemical treatment disorganized the outer membrane of P. aeruginosa which resulted in higher permeability, like "drilling", which significantly improved electron transfer for high power density MFCs. Cyclic voltammograms (CV) of the anaerobically activated control and perforated P. aeruginosa cells were measured in glucose-free medium and showed two pairs of redox peaks at-0.59 and-0.47 V. The two redox waves represented different electron transfer mechanisms of this bio-anode. The output current profiles at a constant load of chitosan, EDTA-and PEI-treated cells were much higher than in control cells, and the PEI-treated cell catalysts produced the highest discharge (oxidation) current density, which parallels the result of the CV experiment. The polarization curves revealed that the PEI-treated cell has much lower polarization for better energy conversion efficiency than the control cells. It was explained that the increased permeability of the bacterial outer membrane by the large pores and channels facilitates the transport of redox mediators and catabolic enzymes through the cell membrane and thus provides faster electron transfer [61].
Introducing a pH-sensitive fluoroprobe into the anode chamber revealed a strong pH gradient within the G. sulfurreducens anode biofilm. The pH decreased with increased proximity to the anode surface and from the exterior to the interior of the biofilm pillars. pH levels near the anode surface were as low as 6.1 compared to pH 7 near the cathode. Various controls demonstrated that proton accumulation was associated with current production. Decreasing the pH of the culture medium from 7 to 6 limited the growth of G. sulfurreducens. The results demonstrated that it is feasible to non-destructively monitor the activity of anode biofilms in real time. It was also suggested that the accumulation of protons that are released from organic matter oxidation within anode biofilms can limit current production [62].
Millo et al. employed surface-enhanced resonance Raman (SERR) spectroscopy in combination with CV analysis. This technique, performed under strict electrochemical control, reveals the redox, coordination and spin states of the heme iron in addition to the nature of its axial ligand. Furthermore, by applying in situ SERR spectroscopy electrochemical analysis on a catalytically active biofilm grown on silver electrodes, it was revealed that two bis (histidine) coordinated heme cytochrome redox couples are involved in the mediated electron transfer between the bacteria and the electrode [63].
Liu et al. showed that graphene modification improved power density and energy conversion efficiency by almost 3 times that of a P. aeruginosa mediator-less MFC. Raman analysis revealed that the improvement was credited to the high biocompatibility of graphene which promotes bacterial growth on the electrode surface and results in the creation of more direct electron transfer activation centers and stimulates excretion of mediating molecules for higher electron transfer [65]. Raman spectroscopy analysis at different growth stages revealed changes in the vibrational properties of Geobacter Cyt c resulting from shifts in the anodic potential between different redox conditions [65].
In conclusion, the biofilm strain composition, components, morphology and thickness play a major role in the electrochemical process in MFCs, and show marked influence on bioelectricity production. Changes in biofilm parameters may influence the anode electrochemical characteristics and performance in a MFC. It is therefore important to understand the characteristics, structure and composition of the biofilm in order to ensure optimized operation of MFCs.