Radiosensitization Strategies Through Modification of DNA Double-Strand Break Repair

of hyper‐ thermia on various repair enzymes and and its relationship to radiosentizing effects under various conditions.


Introduction
DNA double-strand break (DSB) is considered most critical type of DNA damage. In eukaryote, DSB is repaired mainly through non-homologous end-joining (NHEJ) and homologous recombination (HR). Our understanding on the molecular mechanisms of these DNA repair mechanisms has been greatly deepened in the last two decades.
In NHEJ, DSB is first recognized by Ku protein (Fig.1 (1)), heterodimer consisting of Ku70 and Ku86 (also known as Ku80), which in turn recruits DNA-PK catalytic subunit (DNA-PKcs) ( Fig.1 (2)). The comprex consisting of Ku70, Ku86 and DNA-PKcs is termed DNA-dependent protein kinase (DNA-PK). When the DSB are not readily ligatable, processing takes place prior to ligation ( Fig.1 (3)). Processing might involve a number of enzymes depending on the shape of each DNA end and compatibility of two ends to be ligated: Artemis nuclease, DNA polymerase µ/λ, polynucleotide kinase/phosphatase (PNKP), Aprataxin (APTX) and Aprataxin and PNKP-like factor (APLF, also known as PALF, C2orf13 or Xip1). DSBs are finally joined by DNA ligase IV, which is in tight association with XRCC4 ( Fig.1 (4)). XRCC4-like factor (XLF, also known as Cernunnos), is essential at this step, especially when two ends are not compatible.
In HR, a complex consisting of Mre11, Rad50 and Nbs1, termed MRN complex, is thought to play two important roles in the initial stage ( Fig.1 (1')): recruitment of ATM ( Fig.1 (2'')) and resection of one of the strands ( Fig.1 (2')). ATM is a protein kinase structurally similar to DNA-PKcs. Although ATM is thought to phosphorylate a great number of proteins as revealed by phosphoproteomic analyses, the phosphorylation of histone H2AX at Ser139 is thought one of the most important events, triggering signal transduction cascade involving mediator protein like MDC1 and ubiquitin ligases like RNF8 and RNF168. As Mre11 bears 5'-3' exonuclease activity, MRN resects one of the DNA strands to generate single-stranded DNA (ssDNA), which serves as a probe for the search for homology. Replication protein A (RPA) binds to ssDNA ( Fig.1 (2')) and facilitate the formation of Rad51 filament in cooperation with BRCA2, PALB2, Rad52 and Rad51 paralogues ( Fig.1 (3')). RPA also recruites ATRIP, which in turn recruits ATR, another protein kinase structurally related to DNA-PK and ATM ( Fig.1 (3'')). ATR phosphorylate checkpoint kinase Chk1 to initiate signal transduction pathway leading to cell cycle checkpoints. Rad51 promotes strand exchange between homologous sequences ( Fig.1 (4')). Template-dependent strand synthesis is proceeded by replication machinery including PCNA and DNA polymerase δ and ε ( Fig.1 (5')). Finally, the junction of two DNA molecules (Holliday's junction) are resolved by nucleases Mus81-Eme1, ERCC1-XPF or SLX1-SLX4 ( Fig.1 (6')). Alternatively, synthesized strand aneals with opposite end of DSB, detaches from the temprate strand, followed by synthesis and ligation of complementary strand (synthesis-dependent strand anealing; SDSA, not shown here).
Here, we will overview approaches to radiosensitization through the modification of DSB repair enzymes.

DNA-PK
DNA-PK was initially found in the extracts of HeLa cell, rabbit reticulocyte, Xenopus egg and sea urchin egg (Walker et al., 1985) and was purified from Hela cell nuclei as a 300-350 kDa protein, which is now called DNA-PKcs (Carter et al., 1990;Lees-Miller et al., 1990). Later it was found that Ku is an essential component of DNA-PK (Dvir et al., 1992(Dvir et al., , 1993Gottlieb and Jackson, 1993). Furthermore, it was also shown that DNA-PK requires binding of DNA-PKcs to DNA ends via Ku to be activated, suggesting its possible role in sensoring DSBs (Gottlieb and Jackson, 1993). Ku86 was shown to be equivalent to XRCC5 (X-ray repair cross complementing) gene product, which is missing in X-ray sensitive rodent cell lines including xrs-5, -6, XR-V9B and XR-V15B (Taccioli et al., 1994;Smider et al., 1994). Subsequently, DNA-PKcs was found to correspond to XRCC7, which is deficient in scid mouse as well as several radiosensitive cultured human and rodent cell lines (Kirchgessner et al., 1995;Blunt et al., 1995;Peterson et al., 1995;Lees-Miller et al., 1995). DNA-PK is abundant in human cells and its activity can be measured using synthetic peptides derived from p53 (Lees-Miller et al., 1992), enabling extensive studies on its biochemical properties even before molecular cloning of DNA-PKcs.
Sequence of DNA-PKcs revealed its similarity to phosphatidylinositol 3-kinase (PI3K) (Hartley et al., 1995). This study also that fungal metabolite wortmannin ( Fig.2 B), which had been known as an inhibitor of PI3K, could inhibit DNA-PK (Hartley et al., 1995). IC 50 of wortmannin is reported to be 0.016 µM and 0.12 µM (Sarkaria et al., 1998;Izzard et al., 1999). It was also shown that wortmannin binds covalently to DNA-PKcs and functions as non-competitive, irreversible inhibitor of DNA-PK (Sarkaria et al., 1998;Izzard et al., 1999). Expectedly, a number of studies have demonstrated radiosensitizing effects of wortmannin but there is a concern whether the observed radiosensitization was really due to inhibition of DNA-PK. In this regard, some studies showed that radiosensitization by these compounds could be observed even in DNA-PKcs-deficient cells (Rosenzweig, et al., 1997;Hosoi et al., 1998), indicating that radiosensitization by these compounds was not solely due to inhibition of DNA-PK. In addition to ATM discussed next, PI3K-Akt pathway, which might be even more sensitive to wortmannin, might be important to sustain cell survival after irradiation. On the other hand, there are also studies showing that radiosensitization was not observed in DNA-PKcs deficient cells (Chernikova, et al., 1999;Hashimoto, et al., 2003). These studies argue that, even if wortmannin affect PI3K or other kinase more potently than DNA-PK, the radiosensitizing effect might be mainly due to inhibition of DNA-PK. Another PI3K inhibitor LY294002, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one ( Fig.2 C) was also shown to inhibit DNA-PK. In contrast to wortmannin, LY294002 competes with ATP (Izzard et al., 1999). IC 50 of LY294002 is reported to be 6 µM (Izzard et al., 1999). LY294002 was used as a leading compound to explore more potent and selective inhibitors of DNA-PK. NU7026, 2-(morpholin-4-yl)-benzo[h]chromen4-one ( Fig.2 D), was found as selective inhibitor of DNA-PK (Veuger et al., 2003). IC 50 of NU7026 was 0.23 µM for DNA-PK, 13 µM for PI3K and >100 µM for ATM and ATR (Veuger et al., 2003). NU7026 sensitized cultured cells toward radiation in a manner dependent on DNA-PK (Veuger et al., 2003). Synthesis and screening of chromen-4-one library resulted in identification of NU7441 ( DNA-PK can be inhibited by homopolymeric phosphorythioate oligonucleotides, suramin and heparin (Hosoi et al., 2002). Inhibitory activities of homopolymeric phosphorothioate oligonucleotides on DNA-PK were independent of base composition but were dependent on length. IC 50 decreased as length increased: 0.975 µM for 12 mer and 0.013 µM for 36 mer (Hosoi et al., 2002). IC 50 of suramin and heparin were 1.7 µM and 0.27 µg ml -1 , respectively (Hosoi et al., 2002). Suramin sensitized cultured human cancer cell toward ionizing radiation but not to ultraviolet radiation . Furthermore, suramin did not affect the radiation sensitivity of scid cells, which are deficient in DNA-PK, indicating that radiosensitizating effects of suramin were mediated through inhibition of DNA-PK .
Single chain antibody variable fragment (scFv) is another approach to achieve specific inhibition of DNA-PK. ScFv was initially generated from existing murine monoclonal antibody 18-2, expressed in E. coli and introduced into the cell by microinjection (Li et al., 2003). The epitope of scFv 18-2 was mapped within 2001-2025 region, which is outside of kinase domain and thus ScFv 18-2 inhibited DNA-PK activity only modestly (Li et al., 2003). Nevertheless, microinjection of scFv 18-2 resulted in the inhibition of NHEJ, indicated by persistence of γ-H2AX foci and sensitized cells toward ionizing radiation (Li et al., 2003). However, the use of scFv as clinical radiosensitization might be difficult without a method to deliver it efficiently into the cell nucleus. ScFv 18-2 conjugated with nuclear localization signal was developed (Xiong et al., 2009). In more recent study, scFv was conjugated with folate and introduced into the cell nucleus via folate receptor-mediated endocytosis and exhibited radiosensitization in terms of clonogenic survival (Xiong et al., 2012). Another study screened a phage-displayed library of humanized scFv and identified a new antibody against DNA-PKcs, anti-DPK3-scFv (Du et al., 2010). Transfection of cDNA of anti-DPK3-scFv into human cancer cells resulted in increased radiosensitivity with decreased repair capability (Du et al., 2010). It also sensitized transplanted tumor on mice toward radiation (Du et al., 2010).

ATM
ATM, ataxia-telangiectasia mutated, was identified as the gene responsible for the genetic disorder ataxia telangiectasia, showing similarity to PI3K (Savitsky et al., 1995). Subsequently similarity between ATM and DNA-PKcs, suggesting ATM might also be a protein kinase rather than a lipid kinase (Hartley et al., 1995). ATM was shown to be a protein kinase, which is activated by DNA damage and phosphorylates p53 at Ser15  Like DNA-PK, ATM was also shown to be inhibited by wortmannin with IC 50 of 0.15 µM (Sarkaria et al., 1998). It was also shown that administration of wortmannin to cultured cell phenocopies the defect of ataxia telangiectasia cell, e.g., defective accumulation of p53 (Price and Youmell, 1996) and radioresistant DNA synthesis, which is thought to reflect defective G1/S-or S-phase checkpoint (Hosoi et al., 1998;Sarkaria et al., 1998). Caffeine, which was known to abrogate cell cycle checkpoint, was shown to inhibit ATM and ATR (Sarkaria et al., 1999;Hall-Jakson et al., 1999). IC 50 for ATM and ATR was 200 µM and 1,100 µM, respectively (Sarkaria et al., 1999).
CGK733 was reported to be a dual inhibitor of ATM and ATR, but the report was retracted thereafter because of fabrication. Even after the retraction, CGK733 was marketed as an inhibitor of ATM and ATR and several studies used CGK733 to show the involvement of ATM and/or ATR in response to DNA damage caused by a variety of agents. On the other hand, however, there is a report that this compound did not affect ATM and ATR kinase as shown, respectively, by ionizing radiation-induced phosphorylation of ATM at Ser1981 and Chk2 at Thr 68 and by ultraviolet radiation-induced phosphorylation of Chk1 at Ser317 (Choi et al., 2011, and references therein).

ATR
ATR was initially identified as a molecule structurally related to human ATM and yeast Rad3 (Cimprich et al, 1996;Keegan et al., 1996). ATR was then shown to be a protein kinase, which is capable of phosphorylating itself and p53 at Ser15 (Canman et al., 1998). ATR is thought to be a sensor of single-stranded DNA (ssDNA), binding to RPA (Replication Protein A) via ATRIP (ATR-interacting protein) (Zou and Elledge, 2003).
Despite of its structural similarity to DNA-PKcs, ATM and PI3K, ATR appeared refractory to wortmannin inhibition: IC 50 of wortmannin for ATR was 1.8 µM, which was 10-to 100-fold higher than that for DNA-PKcs and ATM (Sarkaria et al., 1998). Selective inhibitors of ATR emerged recently.

Figure 4. Structure of ATR inhibitors
Schisandrin B is an active ingredient of Fructus schisandrae, which has been used in traditional Chinese medicine to treat hepatitis and myocardial disorders (Fig.4 A). Schisandrin B was found to inhibit ATR (Nishida et al., 2009). IC 50 of Schisandrin for ATR and ATM were, respectively, 7.25 µM and 1,700 µM and DNA-PK, PI3K and mTOR were not inhibited up to ~100 µM (Nishida et al., 2009). Schisandrin B sensitized cultured human cells to ultraviolet radiation and ionizing radiation at concentrations 1 -30 µM (Nishida et al., 2009). Sensitization was not observed in cells from Seckel patient, who harbor mutation in ATR gene (Nishida et al., 2009), showing that the sensitizing effect is mediated through ATR.
Library of 623 compounds, which had exhibited some inhibitory effects on PI3K, was screened for their effects on in cellulo phosphorylaiton of H2AX stimulated by ATR-activating domain of TopBP1 . This screening identified NVP-BEZ235 (Fig.4 B) and ETP-46464 ( . This could be due to simultaneous inactivation of two checkpoint pathways mediated through ATM and ATR, respectively, the former of which involves p53. As most of cancer cells lose p53 function, inhibition of ATR might be a promising approach to achieve selective killing of cancer cells, minimizing the effects to surrounding normal cells.

MRN complex
Mirin, Z-5-(4-hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one (Fig.5), was identified in a screen for smalll molecules inhibiting MRN-ATM pathway (Dupre et al., 2009). Restriction enzyme-digested plasmid was added to cell-free extract prepared from Xenopus laevis egg in 96-well format and the phosphorylation of H2AX-mimicking peptide was quantified. Approximately 10,000 compounds, which had exhibited inhibition of p53 activity or inteference with mitosis and spindle dynamics, were subjected to screen. Mirin inhibited H2AX phosphorylation in Xenopus laevis egg cell free extract with an IC 50

DPYD as a new target
Gimeracil, 5-chloro-2,4-dihydroxypyridine (Fig.7), is an inhibitor of dihydropyrimidine dehydrogenease (DPYD) and used as a component of oral anti-cancer medicine S-1, in order to suppress degradation of 5-fluorouracil. The results of clinical trial of concurrent chemoradiotherapy using S-1 suggested possible radiosensitizing effect of S-1. Gimeracil increased radiosensitivity of cultured human cancer cells of various origin within 200 -5,000 µM, being maximal within 1,000 -5,000 µM range (Takagi et al., 2010). Cell lines deficient for DNA-PKcs or Ku86 were sensitized by gimeracil to radiation even to a greater extent than respective control cells (Takagi et al., 2010). On the other hand, radiosensitiztion was not observed in cell lines deficient for XRCC3, NBS1 or FANCD2 (Takagi et al., 2010). These observations collectively suggested that gimeracil exert radiosensitizing effects through inhibition of HRmediated DSB repair. Gimeracil reduced the frequency of homologous recombination of chromosomal substrate including the restriction site of I-SceI by approximately 15% (Takagi et al., 2010). Gimeracil reduced the formation of ionizing radiation-induced foci of Rad51 and RPA but increased that of Nbs1, Mre11, Rad50 and FancD2 (Sakata et al., 2011). This observation suggested that gimeracil might have inhibited the step after strand resection by Mre11-Rad50-Nbs1 complex but before the loading of RPA and Rad51 onto single-stranded DNA. Although the role of DPYD in HR has not been described, treatment with siRNA for DPYD sensitized cells to ionizing radiation to a similar extent to gimeracil and also diminished the radiosensitization by gimeracil (Sakata et al., 2011). These results collectively indicate that gimeracil exerts radiosensitizing effects through inhibition of DPYD, which might have a novel role in HR.

Radiosensitization by hyperthermia
Hyperthermia, heating parts of body at 40 -45 o C, has been used to treat cancer mostly combined with ionizing radiation. Hyperthemia is known to sensitize cells to ionizing radiation, inhibiting the repair of DNA damages including DSBs, but the molecular mechanism of radiosensitization by hyperthermia has remaind to be clarified.
Effects of hyperthermia on DNA polymerases α and β have been studied for a long time. These studies suggested that DNA polymerase β was sensitive to hyperthermia and its inactivation was correlated to radiosensitization as well as to cell killing (Spiro et al., 1982). Later it was reported that DNA polymerase β knocked out cells or overexpressed cells exhibited radiosensitization by hyperthermia indifferent from control cells . Elucidation of DSB repair mechanisms through NHEJ and HR provided clues to the mechanisms of radiosensitization by hyperthermia.
Among essential factors in NHEJ, Ku is shown to be affected by hypethermia. Purified DNA-PK lost its activity upon incubation at 44°C for 5 -30 min (Matsumoto et al., 1997). When DNA-PKcs and Ku were heated separately, heating of Ku, but not DNA-PKcs, lead to decrease in DNA-PK activity, suggesting that Ku, rather than DNA-PKcs, is heat sensitive component (Matsumoto et al., 1997). Inactivation of DNA-PK activity by hyperthermia was observed also in cellulo, i.e., when culture cells were heated at 44 -47°C (Burgman et al., 1997;Ihara et al., 1999;Umeda et al., 2003). It might be noted, however, that the extent of the loss of DNA-PK was greatly different between mouse, hamster and human cell, being greatest in mouse and least in human (Umeda et al. 2003). In murin cells, significant loss of DNA-PK activity was observed at lower tempertures, i.e., 41°C or 42°C (our unpublished observations). DNA-PK activity could be restored by mixing the lysate of heated cells with the lysate of DNA-PKcsdeficient cells, but not with Ku86-deficient cells, indicating that in cellulo inactivation of DNA-PK by hyperthermia might be also due to the property of Ku rather than DNA-PKcs (Ihara et al., 1999). Moreover, Ku was identified as constitutive heat shock element-binding factor, CHBF, whose activity was lost by hyperthermia, allowing the binding of HSF1 (Kim et al., 1995). DNA binding activity of Ku correlated with extent of radiosensitization by hyperthermia (Burgman et al., 1997). Reduced solubility of Ku in aquaous buffer after hyperthermia was also reported, which might reflect aggregation . However, the hypothesis that radiosensitization by hyperthermia is due to inactivation of Ku or DNA-PK has been challenged by genetic studies, showing that cells deficient for Ku or DNA-PKcs could be radiosensitized by hyperthermia to a similar extent or even to a greater extent than control cells (Kampinga et al., 1993;Raaphorst et al., 1993;Woudstra, et al, 1999, although there are studies, in contrast, showing no or reduced radiosensitization in Kuor DNA-PKcs-deficient cells (Iliakis and Seaner, 1990;O'Hara et al., 1995). Moreover, chicken lymphocyte DT40 derivative lacking Ku70 and Rad54, therefore, deficient in both of NHEJ and HR, still showed radiosensitization by hyperthermia Yin et al., 2004).
There is also accumulating studies on the effects of hyperthermia on MRN complex. It was initially found that Mre11, Rad50 and Nbs1 exported from nucleus to cytoplasm upon hyperthemia at 42.5°C or 45.5°C (Zhu et al., 2001;Seno and Dynlacht, 2004). This nuclear export of MRN complex increased when cells were irradiated prior to hyperthermia (Zhu et al., 2001;Seno and Dynlacht, 2004). Similar phonomenon was observed in mild hyperthermia at 41.1°C (Xu et al., 2002). However, in a recent study, inhibition of nuclear export of MRN complex by leptomycin B did not diminish radiosensitization by hyperthermia at 45.5°C for 10 min (Dynlacht et al., 2011). It was also shown, nevertheless, that ATLD cells, which have mutated in Mre11, did not show radiosensitization by hyperthermia at 41.5°C for 2 hrs or at 45.5°C for 10 min (Dynlacht et al., 2011). On the other hand, radiosensitization by hyperthermia was observed in NBS cells and Rad50knocked down cells (Dynlacht et al., 2011). Exonuclease activity of Mre11 was decreased to ~10% by 42.5°C treatment for 15 min (Dynlacht et al., 2011). These results collectively indicate Mre11 as target for radiosensitization by hyperthermia.
Hyperthermia is shown to affect BRCA1 and BRCA2. Heating cultured human cancer cells at 42°C for 1 -2 hrs or more decreased the amount of BRCA1 (Ma et al., 2003). It might be caused by protein degradation, but various inhibitors of proteases, so far as tested, failed to suppress the decrease of BRCA1 (Ma et al., 2003). Alternatively, it might be caused by protein aggregation and reduced solubility in aqueous buffers. It was also shown that BRCA1 deficient cells were sensitive to hyperthermia (Ma et al., 2003). Recent study reported the degradation of BRCA2 induced by mild hyperthermia at 41°C to 42.5°C (Krawczyk et al., 2011). Rad54deficient ES cells and cells treated with XRCC3 siRNA were not radiosensitized by mild hyperthermia (Krawczyk et al., 2011). Furthermore, mild hyperthermia showed synthetic lethality with PARP-1 inhibitor oraparib, like BRCA2 deficient cancer cells (Krawczyk et al., 2011). These data collectively indicated BRCA2 as a major target of mild hyperthermia.
Obviously, hyperthermia inactivates many enzymes and induces aggregation of many proteins. In this regard, hyperthermia is not specific on certain enzyme, unlike inhibitors described above. However, susceptiblity to inactivation by hyperthermia might be greatly different among proteins. The extent of radiosensitization by hyperthermia can be greatly infuluenced by many factors, e.g., cell type, genetic background, physiological conditions, heating temperature, duration of heating, sequence of heating and radiation and the interval between them. Further studies would be required to examine the effects of hyperthermia on various repair enzymes and and its relationship to radiosentizing effects under various conditions.

Concluding remarks and future perspectives
Because of great advances in our understanding of the moleuclar mechanisms of DSB repair in past two decades, extensive studies have been done to achieve radiosensitization by modification of DSB repair molecules. Especially, a number of inhibitors have been developed for DNA-PK, ATM and ATR protein kinases. We saw here that preceeding studies on DNA-PK and on PI3K greatly facilitated the studies on ATM and ATR. It might be underscored that LY294002, preexisting inhibitor of PI3K, served as a leading compound and enabled the finding of potent and specific inhibitors like NU7441 and KU-55933.
Studies toward the clinical application of these compounds are underway. Preclinical studies of pharmacokinetics and metabolism in mice were conducted for NU7026 and NU7441. In the case of NU7026, the radiosensitizing effect on cultured cancer cell was marginal upon the treatment at 10 µM for 2 hrs and could be increased by extending the treatment time up to 24 hrs (Nutley et al., 2005). On the other hand, however, NU7026 underwent rapid plasma clearance in mice, presumably because of oxidation and ring opening of morpholino group (Nutley et al., 2005). It was estimated that NU7026 should be administered four times per day at 100 mg/kg intraperitoneally in order to obtain radiosensitization (Nutley et al., 2005). In the case of NU7441, the radiosensitizating effect on cultured cancer cell could be obtained by treatment at 1 µM for 1 hr (Zhao et al., 2006). The concentration of NU7441 required for radiosensitization could be maintained within tumor tissues for more than 4 hrs at nontoxic dose (Zhao et al., 2006). The administration of etoposide and NU7441 to mice bearing human tumor xenografts synergistically delayed tumor growth, indicating the chemosensitizing effect of NU7441 in vivo (Zhao et al., 2006). Studies are still going on to obtain compounds with better characteristics, e.g., higher aquaous solubility (Cano et al., 2010).
Search for inhibitors of enzymes other than protein kinases has been difficult due to the absence of assay system suitable for highthroughput screening. However, inhibitors of other enzymes, i.e., Mre11 nuclease and DNA ligase IV have been developed, although few at present. Now these compuonds are obtained, more potent and specific inhibitors can be obtained by molecular evolution as in the case of DNA-PK, ATM and ATR protein kinases. Additionally, search for other inhibitors will be greatly facilitated by an aid of computer-based structural prediction and drug designing.
In addition to use of each chemicals alone, use of two or more chemicals together to inhibit two pathways of DSB repair or one of them with other repair mechanisms, which is called synthetic lethality approach, will be promising. Successful example is shown in the treatment of cancers arisen in the carriers of BRCA2 mutation with PARP-1 inhibitors. When PARP-1, which is essential for single-strand break (SSB) repair, is inactivated, SSB is converted to DSB, which requires BRCA2 to be repaired. As BRCA2 mutation is heterozygotic, normal cells retain BRCA2 function. On the other hand cancer cells have lost BRCA2 function and, therefore, shows extreme sensitivity to increased sensitivity to converted DSBs. This is instructive also to find a means to discriminate cancer cells and normal cells. As described in the previous section, although hyperthemia is not an approach to target a certain molecule specifically, it did show synthetic lethal effects with PARP-1 inhibitor. These examples underscores the importance of the choice of agents based on the thorough consideration of biological characteristics and genetic background of each cancer and patient. In addition to continuing persuit for the new radiosensitizing agents, extensive studies would be necessary regarding combinatorial approach and personalized medicine.

Note
We apologize for not citing many important literatures because of space limitation. kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell, 80: 813-823.