Synthesised recommendations extracted from think aloud protocol and digital content extraction.
\r\n\tNot all mixtures of particles and liquids can be considered slurries. A slurry has its character quite different from the carrying liquid (sometimes referred to as the vehicle). A Newtonian liquid has its shear stress directly proportional to its rate of deformation, but this is seldom the case for a slurry. In general, slurries are referred to as non-Newtonian liquids and ways of dealing with them are important threads in this text.
\r\n\r\n\tPipe blockages and pipe wear cause high costs to industry, in both maintenance and loss of production. This waste, and environmental damage which comes with it, can be shown to be reduced by careful application of slurry technology. This book will welcome recent research efforts to understand slurries related to the above-mentioned topics.
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For example, Ireland’s OA population increased by 19% in recent years [2]. This situation reflects that in most countries lifestyles have improved, however it does go hand in hand with impacting long-term care and welfare systems [3]. There are various definitions for what constitutes an older adult, and of course there is not a particular day in which someone moves from being a younger or middle aged adult to an older adult [4]. For the purpose of our study, however, we define OAs as someone who has reached the age of 50, basing our work on established studies e.g. TILDA2 [5]. Additional qualifications to be included in our study is that the OA is already motivated to use smartphone technology.
The quality of life of OAs can be considerably improved through active social engagement, improved healthcare, and increased mobility through access to transportation [6]. Technology offers a ray of hope in providing access to these opportunities. But, the adoption rate of technology by OAs appears low [7]. Technology comes in many forms, to include public displays, virtual reality, websites and smartphone applications. As numerous technologies may have a different set of design requirements, our focus will be on smartphone applications only – smartphones are frequently used by OAs [8]. Furthermore, smartphone applications can help to provide access to digital services with no or low-cost [9].
To develop smartphone applications for OAs, designers must recognise the individual needs of this group such as the varying degrees of physical and cognitive decline [10, 11] and privacy concerns [12]. These specific features are not always considered, and a recent study advocates for better recommendations for age-friendly design of user interfaces on mobile phones [13]. A tried and tested way to elicit such specific recommendations is through participatory or human-centred development [14]; shown as a successful way to develop such guidelines [15] and checklists [16, 17], and for interface design of mobile phones for older adults [18]. However, existing guidelines deal with visual and haptic issues and omit many elements associated with the textual interface. Additional shortcomings of these guidelines are that they are rarely tested [15, 18] and lack extensive empirical validation [19]. Also, the guidelines for designing applications for OAs are not presented to designers and developers in an accessible format [20] and are sometimes merged in the discussion section of the papers e.g., [19, 21, 22]. A recent literature review summed up existing guidelines as piecemeal, lacking in characterisation, not easily actionable, and rarely validated [23]. In summary, the conflicting and disparate advice offered, results in arguably unreliable and hard to follow guidelines.
In this chapter, we focus particularly on the Usability recommendations for smartphone applications, for designing products to be effective, efficient, and satisfying. Usability includes user experience design. In our context, usability covers general aspects that impact everyone and do not dis-proportionally impact people with disabilities [24]. Furthermore, we look for better ways to disseminate our guidelines by transferring the recommendations into a set of patterns. This form of reporting is shown to lead to increased adoption of guidelines by practitioners [23, 25, 26].
We respond to the call to create a design patterns library aimed towards practitioners and pedagogical settings [20].
In summary, a few attempts have been made by the research community to produce guidelines or checklists, but for the most part they have not been validated or applied in practice [27]. Also, their implicit nature lacks explanation on how to apply these recommendations. Henceforth, this research fills this void by providing a structured and validated set of design patterns to support the design and development of usable smartphone applications for tech-savvy OAs. Our study aims to answer:
The next sections of this study are organised as follows. Section 2 describes the four different stages within the Research Methods and how the collected data was analysed. After that, Section 3 presents the results of execution of these stages and Section 4 depicts the transformation of findings i.e. recommendations in the form of design patterns. Section 5 then highlights how some of the threats to validity were catered for whilst conducting this research. Section 6 concludes this study and points towards potential future directions.
Data collection was conducted in two phases, firstly through Think Aloud sessions, followed by Digital Content extraction.
The primary objective of the first phase i.e., Think Aloud (TA) sessions, was to understand the expectations of tech-savvy older adults regarding a set of smartphone applications. Think Aloud Protocol is a tool borrowed from cognitive science [28]. There are numerous variations of this method for Usability evaluation of technology. However, there is no consensus about the exact procedure to perform these sessions [29, 30]. These are usually performed by asking individuals to think out loud whilst interacting with the system [31]. If the user stops talking for long, then a gentle reminder is used to prompt him/her to start conversing again. The other option is to have a pair of users verbalise their thoughts retrospectively rather than concurrently [32]. For this study, the Think Aloud Process of [33] was used for conducting the sessions (as described below), because it is well established, widely used, and helps in Usability testing as shown in Figure 1. OAs in our study were given the liberty to choose two smartphone applications (already installed on their smartphones), use them, and highlight the good and bad things about them. Author 1 observed each OA and took field notes as they navigated through their chosen apps.
Think aloud process [
The second phase to further understand tech-savvy OA’s user-interface needs was digital content extraction that comprised two sub-phases:
We coded and classified the recommendations into a structured vocabulary using a deductive approach. The recommendations were mapped into seven categories provided in Peter Morville’s [37]. Usability honeycomb as shown in Figure 2. Usability is about designing products to be effective, efficient, and satisfying. Usability includes user experience design. This may include general aspects that impact everyone and do not dis-proportionally impact people with disabilities. The caveat here is that usability practice and research often does not sufficiently address the needs of people with disabilities [24].
User experience honeycomb [
We also considered two alternatives to Morville’s definition of Usability. Firstly, in 1994, Jakob Nielsen [38] provided 5 attributes that together constitute usability i.e. Learnability, Efficiency, Memorability, Errors and Satisfaction. Then in 2003, Whitney Quesenbery [39] proposed a 5E model to describe usability i.e. Effective, Efficient, Engaging, Error Tolerant and Easy to Learn. Subsequently, in 2004, Peter Morville used a ‘honeycomb’ to illustrate usability with 7 sub-categories Useful, Usable, Findable, Valuable, Desirable, Accessible, Credible. The reason we choose the latter is that it is the latest and more granular than the former ones i.e. 7 categories compared to 5, and it is well recognised and highly cited.
Different coefficients can be used for evaluating the agreement in classification of recommendations between the three raters or inspectors.
Once we identified and developed a consolidated set of recommendations, we structured them into a design pattern format which consisted of sections: Problem, Rationale, Solution, Type, Sub-type, Related Patterns, References/Evidence as shown in [36], which explains each heading and what is included in each section.
Qualitative evidence suggests that design patterns can aid maintenance, but they do not appear to help novice designers learn about design [49]. Also, Bieman particularly observes that: ‘there is very little empirical evidence of the claimed benefits of design patterns and other design practices when applied to real development projects’ [50]. This implies that simply using patterns does not ensure good design, they should be used appropriately [49]. The initial attempt to develop design patterns was made by Christopher Alexander to document and discuss building architecture design and solutions [51]. Design patterns have become popular among software developers after the publication of “Gang of Four” [52]. It is defined as:
There are subtle differences in the structure of the design patterns used in existing research. This paper will follow the format proposed by [54] as this is also used in numerous studies e.g., [53, 55, 56].
In qualitative research, the process of data collection, data analysis, and report writing is not always completed in distinct steps; they are often interrelated and occur simultaneously throughout the research process [57]. We used thematic analysis to develop themes that were emphasised during Think Aloud sessions or on the ageing forums. This is a method for identifying, analysing, organising, describing, and reporting themes found within a data set. Bruan et al. 2006 [58] presents a linear, six-phased method for thematic analysis. This involves becoming familiar with the data, generating initial codes, searching for themes, reviewing themes, defining and naming themes and producing the report. It is actually an iterative and reflective process that develops over time and involves a constant moving back and forward between phases. We have adapted the guidelines of [57, 58] to perform analysis of the qualitative data generated during the mixed methods. This was accomplished using Microsoft Excel. The snippets were matched with the theme columns and marked or annotated using zero, one, tick, cross. To facilitate code creation, we used a single ‘sentence’ as a unit of analysis for extracting and defining the themes. Author 1 developed an initial set of themes which were validated by author 3.
Ten OAs, five female and five male, who had English as their primary language, participated in Think Aloud sessions. All of them had access to basic technology such as landline, internet and smartphones. Their median age was 64.7. Eight were fully or partially retired and two were working professionals. OAs were delighted to talk about the apps they use on a regular basis. For instance, the first participant, a retired Civil Engineer, talked about his experience with SmartTools6 app. He said that SmartTools is his favourite app because he can perform basic civil engineering tasks using this simple and easy to use interface showing the tools. The tools include ruler, protractor, level, thread, range-finder, compass, metal detector, sound meter, vibrometer, flashlight, magnifier, mirror and unit converter. He was pleased about the real-world metaphor of ‘Cupboard’ used in this app. Also, he said that the labels used in the app are self-explanatory. On the other hand, he did not like using a budget airline app. He said the initial waiting time, excessive number of advertisements, hectic process to book a flight, small text size, icons without labels, and long and technical terms and conditions make him reluctant to use this app. He stated that this app does not cater for OAs and gave an example of a issue in the form completion section. The salutations, Mr, Mrs, need to be tapped for successful submission of the form, but there is no indication to select them. Also, they are represented in dull colours, reducing their importance in the eyes of the user. An array of recommendations given by this participant was collected such as “video based tutorials in the app to enable the user to become familiar with the app”. Similarly, the analysis of data collected from other participants helped to generate recommendations.
We extracted data in two ways, firstly from online forums in which OAs were engaging with developers and the wider community on issues they were experiencing with their apps (see (A) below), and secondly by testing a sub-set of apps developed especially for OAs (see (B) below).
OAs on these forums appear to be well-versed with technology. They would love to use more of the latest technology if it is inexpensive and privacy is ensured. This is in line with our previous research [59]. One of the older adults mentioned on the forum that he would prefer wearables rather than smartphones. Here is an excerpt from his comment:
OAs constantly discuss how to ensure privacy whilst using technology on these forums. Here is a solution proposed by a member on the question of ensuring privacy when using websites:
A total of 32 mainstream apps were evaluated to find recommendations that are making these apps successful and/or unsuccessful. We started by identifying keywords, joining them together to define search strings. An example search string applied to app stores is ‘apps for older adults’. Author 1 then searched Android and Apple app stores using the defined search strings. An initial search produced 177 and 18 results for the former and latter (these searches were conducted between May to August of 2019). A scrutiny was performed on these retrieved apps using an agreed inclusion and exclusion criteria [36]. This exercise reduced the number of apps, and we were left with a set of 101 android and 6 iOS apps. As the evaluation of 107 apps would have required a herculean effort, and more time than we had available, we selected a sample of apps from this set. In order to reduce the sampling error and achieve precision, we opted for a stratified sampling method. Hence, these 107 selected apps were carefully reviewed, arranged and classified into 9 different categories. These were Health & Safety, Launcher, Dating, Gaming, Shopping, Entertainment, Communication & Socialisation, Education and Tools. After stratification of the apps, a simple random sampling technique was applied on each of the nine sub groups. An equivalent percentage of 30% was selected from each type of app. This step resulted in a total of 32 finally selected apps, 30 android and 2 iOS based.
We analysed the qualitative data generated from Think Aloud Protocol and Digital Content Extraction using the strategy explained in Section 2. Likes, dislikes and recommendations for the development of smartphone applications for tech-savvy older adults were extracted.
1. Likes.
If icons are ugly, older adults get demotivated and do not want to use the application or a particular version where the icons are not attractive.
2. Dislikes.
3. Recommendations.
To reiterate, the Think Aloud Protocol and Digital Content Extraction were conducted concurrently. This produced an exhaustive list of recommendations to develop smartphone applications for tech-savvy OAs. Some of these are described earlier along with sample quotations from the participants. There were a total of 131 recommendations for Usability with some overlapping [36]. However, for the sake of preciseness, a subset of synthesised versions of these recommendations mapped against the source from which they were derived are presented in Table 1. A total of 14 design patterns were created from these recommendations, and 3 of them are presented in this study.
Synthesised recommendations extracted from think aloud protocol and digital content extraction.
Each unique recommendation was mapped by all authors against the 7 Usability categories outlined by Morville’s Usability honeycomb and then validated using Inter-rater reliability as described in Section 2. The scores for inter-rater reliability exceeded 0.61, indicating a high level of reliability. The values of Cohen Kappa’s co-efficient (
Raters | Author 1 and 2 | Author 1 and 3 |
---|---|---|
0.698 | 0.809 |
Cohen’s kappa score for mapping of recommendations.
We developed numerous design patterns for Usability after conducting Think Aloud and Digital Content Extraction. Patterns, derived from these, also include supporting literature, thus providing further information about how to develop applications for OAs. Other authors have used patterns to describe usability design recommendations, such as [20, 26]. We build on their work by applying a range of recommendations in this format. Design patterns are shown to be beneficial [60]:
Improve communication between stakeholders through providing a common vocabulary e.g., developers and maintainers.
Improve programmer productivity and program quality.
Encourage best practices even among experienced designers.
Due to limitations on the size and volume of this chapter, we present a subset of three example design patterns covering sub categories of usability, i.e. Usable, Desirable and Accessible, in Tables 3–5. The remaining set of design patterns can be accessed at [36]. Thus, these design patterns are the answer to the research question posed earlier:
Section | Description |
---|---|
Cognitive decline is one of the common issue related to ageing, making it difficult for older adults to remember complex things. In the context of technology, passwords are troublesome for older adults [1]. | |
The decrease in memory causes older adults to forget and ultimately leads to demotivation. This, in turn, acts as a major factor to stop using the system [1]. | |
1. Make the app accessible without the need for a password. Use alternative access mechanisms such as bio-metrics, finger prints or face recognition or text message to phone number [1]. | |
Usability | |
Usable | |
Incorporate one feature on interface or one question with a progress bar in the application. This is sometimes referred to as gamification. | |
[1] Boyd, K., Bond, R.R., Nugent, C.D. and Donnelly, M.P., 2018. EasiSocial: Recommendations in the development and training of social media tools for older people. In EasiSocial: Recommendations in the development and training of social media tools for older people. |
Make app more accessible with easy log-in id - e.g. fingerprint instead of password.
Section | Description |
---|---|
Older adults get confused by the fast moving objects or content on the smartphone applications. This is mainly due to difficulty associated with the level of perception [1, 2]. | |
Fast moving objects and animations make it challenging for older adults to concentrate. They feel themselves as less able to understand the complexities of the applications and eventually stop using it [1, 2] | |
1.Avoid animations and marquees (e.g., text moving from top to bottom) in the application [1, 2]. | |
Usability | |
Desirable | |
Allow the older adults to choose a preferred theme in the application. | |
[1] De Barros, A.C., Leitão, R. and Ribeiro, J., 2014. Design and evaluation of a mobile user interface for older adults: navigation, interaction and visual design recommendations. Procedia ComputerScience, 27, pp.369–378. [2] Nurgalieva, L., Laconich, J.J.J., Baez, M., Casati, F. and Marchese, M., 2019. A systematic literature review of research-derived touchscreen design guidelines for older adults. IEEE Access, 7, pp.22035–22058. |
Avoid animations and marquees (e.g. text moving from top to bottom) in the application.
Section | Description |
---|---|
Older adults suffering from auditory problems might not be able to hear important notifications or voice-enabled conversation by the application [1, 2, 3, 4]. | |
Hearing loss is one of the key problem that is associated with ageing and the applications should be designed for individuals who suffer from it. This will make the application inclusive, accessible and easy to use [1, 2, 3, 4] | |
1.Incorporate auto-captioning for disabled users in the app [1, 2, 3, 4] | |
Usability | |
Accessible | |
Make the tap sensitivity high to cater for older adults with dry and husky fingers. | |
[1] De Barros, A.C., Leitão, R. and Ribeiro, J., 2014. Design and evaluation of a mobile user interface for older adults: navigation, interaction and visual design recommendations. Procedia ComputerScience, 27, pp.369–378. [2] Nurgalieva, L., Laconich, J.J.J., Baez, M., Casati, F. and Marchese, M., 2019. A systematic literature review of research-derived touchscreen design guidelines for older adults. IEEE Access, 7, pp.22035–22058. [3] Al-Razgan, M.S., Al-Khalifa, H.S., Al-Shahrani, M.D. and AlAjmi, H.H., 2012, November. Touch-based mobile phone interface guidelines and design recommendations for elderly people: A survey of the literature. In International Conference on Neural Information Processing (pp. 568–574). Springer, Berlin, Heidelberg. [4] Leitão, R. and Silva, P.A., 2012. Target and spacing sizes for smartphone user interfaces for older adults: design patterns based on an evaluation with users. |
Incorporate auto captioning for disabled users in the app.
It is imperative to consider limitations and threats to validity of research [61]. This is particularly important for empirical studies in software engineering, where there is often a multitude of possible threats [62]. First and foremost, the targeted population of this study was tech-savvy older adults, so the findings might not be applicable as-is to age-related counterparts who experience further difficulties in learning and adopting new technology. However, additional findings which included non-tech savvy OAs are presented in [36]. Moreover, for construct validity, field notes taken during the two studies were added to the data collection form on the same day of the sessions. To improve participants’ understanding, an information sheet was provided written in English, particularly for Think Aloud sessions. Moreover, Digital Content Extraction involved older adults from across the globe, which may have enhanced the external validity of this research. A known problem with every form of research is that the experience of the researcher can influence both the data collection and analysis positively or negatively. We tried to reduce this bias by involving all three researchers in evaluating the codes and by checking the criteria for data selection. In the context of Think Aloud sessions, the setting was only an approximation of a real-life situation, not the actual day-to-day life usage of technology by older adults. The primary limitation of the examination of online ageing forums was the focus on two forums only. Also, a limited sample of applications was selected from the mainstream app stores using stratified sampling. Despite the limitations, the results follow from the data collected via empirical studies. For reliability, the evaluation of recommendations, through the use of inter-rater reliability, added confidence that the study would yield similar results if other researchers were to replicate our methods.
The empirical studies conducted with tech-savvy OAs highlighted their specific expectations from smartphone applications. Through conducting Think Aloud Sessions and Digital Content Extraction we elicited a set of requirements that we categorised according to Peter Morville’s Usability Honeycomb [37]. To provide a level of objectivity to our classification, we validated our results in a series of inter-rater reliability tests. The output of this study is a set of recommendations for developing smartphone applications for a tech-savvy ageing population. Finally, we transformed the recommendations into 14 design patterns (three of which are presented in Tables 3–5). These patterns will help make these recommendations usable for practitioners or for pedagogy. For academia, the research results aim to provide an understanding of the recommendations for developing smartphone applications for OAs. For industry, the recommendations in the design patterns aim to help the development of usable smartphone applications for tech-savvy older adults, from development to testing of applications. We do not claim that the results presented are complete, but our research study provides significant results, and the design patterns in particular, are ready to be used by both developers and educators.
This work was supported, in part, by Science Foundation Ireland grant 13/RC/2094, co-funded under the European Regional Development Fund through the Southern & Eastern Regional Operational Programme to Lero - the Science Foundation Ireland Research Centre for Software (www.lero.ie) and IBM Ireland. We also thank the participants who gave their time in our ‘think aloud’ sessions.
Precipitation and evapotranspiration are basic compounds of hydrologic cycle. Precipitation is crucial natural source of water in the soil. Precipitation amount and temporal distribution of the rainfall is important for water refilling of the environment for balanced periods. Drying of soil profile occurs during long rainless periods. Meteorological drought and subsequently soil drought occurs in the case of the sufficiently long rainless period. Therefore it is necessary to know size and statistical characteristics of rainless periods (RLP).
\nFor the analysis of the impacts of the length of the non-precipitation period on the soil water regime, it is necessary to analyse the second critical component of the hydrological cycle. It is a process of evaporation and quantification of the vapour. The evaporation of water in nature is physically the process of converting water to vapour. Due to the high vapour heat (2450 kJ kg−1 at the evaporating surface temperature of 20°C) it is an energy-intensive process. In nature, it is the decisive regulator of energy flows. The evaporation from the soil and plants is called evapotranspiration. The intensity of evapotranspiration over time is affected by the power consumption required for the phase conversion of water to vapour and the amount of this water in the environment. Consequently, evapotranspiration is seasonal in the course of the year. The decisive amount of water evaporates in the vegetal half of the year—from April to September. The maximum possible evapotranspiration under given meteorological conditions is potential evapotranspiration (ET0). Its size is the result of the energy balance on the active surface. Really evaporated water from the soil and plants is called the actual evapotranspiration (ETa). It depends on the positive energy balance, the turbulent exchange between the plant and the atmosphere, the water content of the soil profile, and the plant’s ability to regulate the intake and discharge of water from its own organism. If there is sufficient water in the environment, then the actual evapotranspiration is the same as the potential evapotranspiration. If ET0 > ETa, there is an evapotranspiration deficit. This in the root zone of the soil profile indicates the water supply deficit and the beginning of the soil profile drying. Current climate changes are reflected in the redistribution of precipitation over the course of the year and the increase in the air temperature. In other words, the distribution of water and energy in the hydrological system is redistributed. These changes are reflected in changes in evaporation and consequently in changes in the water balance of the area [1, 2, 3, 4, 5, 6]. The authors of the chapter have the hypothesis that not only the height of precipitation but also their time distribution during the year has the effect on the occurrence of soil drought.
\nThe aim of the chapter is to identify significant non-precipitation periods, to quantify their time duration, probability properties, development trends and impacts on changes in the critical components of the water regime in the soil environment. In the course of significant non-precipitation intervals during vegetation periods, the water reserves in the root layer of the soil up to a depth of 1 m, the actual and potential evapotranspiration, the evapotranspiration deficit, the groundwater level and the air temperature were analysed.
\nThe identification of non-precipitation periods and the process of their impact on the components of the water regime of the soil environment were examined in the Milhostov locality in the Eastern Slovak Lowlands (ESL).
\nThe analysis concerned the growing seasons from 1970 to 2015. During the analysis, the following values were analysed at 1-day calculation step and one-day input data: non-rainfall periods, soil water storage to the depth of 1 m; movement of the groundwater level; air temperature; and daily totals of actual and potential evapotranspiration, rainfall and evapotranspiration deficiency.
\nThe research was based on field measurements of hydrophysical characteristics, volume humidity monitoring over the soil profile vertical line, laboratory work and numerical simulation of the water regime using GLOBAL mathematical model [1, 2, 3, 4, 5, 6]. The part of the research works was also the verification of the mathematical model using results of the monitoring carried out in the examined locality.
\nThese hydrological processes were examined in Milhostov, the area located in the central part of the ESL, Slovakia (48°40′11.08″, N; 21°44′18.02″, E; 100 m). The selected area is characteristic of the ESL. Typical soil of the area is gleyic fluvisol, a medium-heavy soil where clay particles forms 11–39% of the content.
\nThe profile is made of clay, clay-loam and silty clay layers Figure 1.
\nTexture of the examined soil profile as defined by the USDA triangle (sand 0.05–2.0 mm, clay <0.002 mm, silt 0.002–0.05 mm).
Figure 2 shows the course of the clay, dust and sand content via the vertical line of the soil profile by 0.1 m layers to a depth of 1.0 m.
\nContent of clay, silt and sand to the soil profile vertical to 1 m depth.
The clay, silt and sand content via the vertical line of the soil profile to 1 m depth. Figure 2 shows that the most ratio of the studied profile is formed by the dusty component. The profile is up to 0.7 m relatively homogeneous. In the range between 0.7 and 1.0 m, the ratio of the individual components of the microstructure significantly changes. For this reason, two material layers were considered in the calculations. Table 1 lists their retention lines parameters according to Van Genuchten.
\nLayer | \nAlpha | \nn | \nθs | \nΘr | \n
---|---|---|---|---|
0.0–0.7 m | \n0.0084 | \n1.5202 | \n0.43 | \n0.12 | \n
0.7–1.0 m | \n0.0482 | \n1.1593 | \n0.39 | \n0.07 | \n
Parameters of retention lines of material layers of the soil profile.
In terms of climate, the examined area, as well as the rest of the ESL, is located in the transitional climate region between the maritime and the continental climate. In terms of temperature, the area is homogeneous. Long-term mean temperature in the area between the years 1961 and 2015 is 9.4°C; minimum mean daily temperature is −20.5°C, maximum mean daily temperature is +30.6°C. The absolute minimum temperature in the area during the analysed period was −29.1°C (January 01, 1987) and the absolute maximum daily temperature was +38.2°C (July 22, 2007). The warmest month is July, the coldest is January. The mean annual amount of rainfall in the area is 558 mm (years 1961–2015). Daily maximum rainfall total was 82.5 mm (June 26, 1995). Rainfall conditions in the area are heavily influenced by the air circulation. Heaviest rainfall is induced by the humid and warm air flowing from the south. Air currents from the other directions do not usually bring rainfall because it falls on the Carpathians. The maximum duration of the continuous period with no rainfall was 35 days. The maximum annual long-term mean duration of the period with no rainfall is 17 days. Long-term mean wind speed 10 m above the ground is 2.468 m s−1. Daily long-term mean pressure of water vapour is 0.999 kPa where the daily mean minimum is 0.083 kPa and the maximum is 2.760 kPa. Daily long-term mean relative humidity is 77.4%, where the daily mean minimum is 33% and the maximum is 100%. With regard to phenological characteristics and vegetation of the area, there are no noticeable temporal or local changes.
\nMethodical process of the research consists of two phases: rainless periods selection, model verification, calculation of water regime components and results interpretation. Climatic station of Milhostov (N 48°39,786′; E 21°43,298′) was chosen for selection of statistically important rainless periods. Station is located at central part of Eastern Slovak Lowland (ESL) and represents wider area of lowland. Daily precipitation amounts of the 1961–2015 period was examined for the station. 20,080 daily precipitation amounts (including zero) were analysed for the period. Length of RLP was identified separately for whole years of examined periods and separately for vegetal periods (VP). Selections of RLP were applied for VP examination in two ways. Periods with zero daily precipitation amounts were considered in first selection (s0). Daily precipitation amounts lower than 2 mm were considered as zero in the second selection (s2). These precipitation are likely to be catched by the plant cover and subsequently vaporised without influencing soil water supply. Selection of rainless periods during VP was done only of those which occurred during vegetal periods (April–September). However, periods that starts before VP and overlap to VP and those that starts during VP and finished after VP were considered as well. Rainless periods of the 1961–2015 were probability-evaluated in every selection group.
\nJust (s0) selection was applied during examination of entire year periods. Basic statistical characteristics were calculated after identification of RLP. Probability characteristics of occurrence of different RLP temporal duration were calculated by binomial distribution. The results are indicated in the form of frequency curves. Development of number of rainless days during individual years and VP was evaluated. For example, 10 longest RLP during 1961–2015 were selected for every selection.
\nThe database for needs of the experiment was collected by the field monitoring, laboratory measurements and the numerical simulation via the mathematical model ‘GLOBAL’.
\nThe field monitoring concerned the measuring of the groundwater level and volumetric moisture within a soil profile, vertically to the depth of 0.8 m and horizontally by 0.1 m thick layers. The field works included also soil sampling. Gathered soil samples were processed in the laboratory and basic characteristics of the soil profile were determined. The Climatic and Agroecology Research Institute is located in the examined area. During the years 1970–2015, the institute provided hydro-meteorological data and plant characteristics necessary for the numerical simulation via the mathematical model ‘GLOBAL’. The results of the water storage monitoring to the depth of 0.8 m are available as well. The model was verified using the data from the extremely dry growing season in 2007. The growing season of 2007 was the second driest season of the analysed period. The driest season was that of the year 2015.
\nOnce the model was verified, the development of
The simulation was conducted for the growing seasons from 1970 to 2015.
The model ‘GLOBAL’ is a mathematical model to enable simulating water movement in soil and calculating the distribution of soil moisture potential, i.e. soil moisture in real time [7, 8, 9, 10, 11, 12]. The model is based on the numerical solution of a non-linear partial differential Richards’ equation describing water movement in aerated soil zone, which is as follows:
where \n
The simulation using the model ‘GLOBAL’ can be performed with one-day calculation step. In that instance, daily values are used as the input values for creating the boundary conditions. This also applies to the meteorological inputs and the plant cover input parameters. The model ‘GLOBAL’ includes also soil hydrophysical characteristics such as: retention curves, soil saturated and unsaturated hydraulic conductivity, hydrolimits and some physical soil characteristics (porosity, specific weight and volumetric mass density and moisture of saturated soil). In the model, hydrophysical characteristics are expressed by analytical equations. Moisture retention curve is described by van Genuchten model.
\nPotential evapotranspiration
The value of empirical coefficient (m1 = 0.463) was gained by field measurements in a maize field. Actual evapotranspiration and its structure is calculated based on the values of potential evapotranspiration ET0 and the relation between relative evapotranspiration Eeo/ET0 and soil profile moisture, i.e.
The calculation method is based, inter alia, on the assumption that the median soil moisture in the root zone used for the calculation of transpiration, or the value of moisture in the upper layer of a soil profile used for the calculation of evaporation, depends on the intensity of evaporation. The higher is the intensity of evaporation, the higher is the value of
Basic characteristics of descriptive statistics are indicated in Table 2. It results that 12,185 days (60.68%) were with no precipitation in 1 year selection data series. Days without rainfall were grouped to 3409 periods. 63.87% of days without rainfall were in the case of VP and s0 selection and 85.12% days without rainfall in the case of VP and s2 selection. The longest RLP duration was 35 days for s0 selection and 53 days for s2 selection.
\nParameters | \nYear | \nVP | \nVP | \n
---|---|---|---|
Selection | \nf(0) | \nf(0) | \nf(2) | \n
Mean | \n3.57 | \n3.67 | \n6.15 | \n
Standard error | \n0.06 | \n0.08 | \n0.17 | \n
Median | \n2 | \n2 | \n4 | \n
Mode | \n1 | \n1 | \n1 | \n
Standard deviation | \n3.52 | \n3.51 | \n6.31 | \n
Sample variance | \n12.41 | \n12.30 | \n39.84 | \n
Kurtosis | \n10.98 | \n9.66 | \n8.47 | \n
Skewness | \n2.64 | \n2.47 | \n2.45 | \n
Range | \n34 | \n34 | \n52 | \n
Minimum | \n1 | \n1 | \n1 | \n
Maximum | \n35 | \n35 | \n53 | \n
Sum | \n12,185 | \n6429 | \n8567 | \n
Count | \n3409 | \n1751 | \n1393 | \n
Confidence level (95.0%) | \n0.12 | \n0.16 | \n0.33 | \n
Basic statistical characteristics of rainless periods.
Eloquent image of probability of occurrence of maximal year durations of RLP gives probability of exceedance curves. Curves are indicated in Figure 3. There of results that only little differences are between course of yearly and vegetal selections of s0. Length of RLP with p = 50% probability of exceedance is for this selection of VP 13 days and for the year 15.5 days. This value is 23.3 days for selection s2 during the vegetal period. The course of probability of exceedance curve is in case of s2 selection moved and more steep. This indicates greater variability and longer duration of RLP.
\nEmpirical and theoretical probability of exceedance curves of maximal year durations of rainless periods.
Numbers of days without rainfall are indicated in Figure 4 for individual years and their vegetal periods for selections s0 and s2. Developmental trends are for all cases parallel with timeline. It is testified by the fact that number of rainy and rainless days is for individual years and their vegetal periods balanced in the long term view. Only duration of rainless events of non-vegetal periods changes in examined periods. It is confirmed by the results that are graphically shown in Figure 5. There are indicated numbers of days of rainless periods that lasts 10 days or more for VP of individual years. From the figure results that trend development of both selections s0 and s2 is stable without change (parallel to the timeline) during vegetal periods. Elongated trend of rainless periods with duration of 9 days or more was identified in case of s0 yearly selection. These results show that this trend is caused by rainless events during non-vegetal periods. Stated fact has influence on creation of water storage during before-spring periods and enlarges risk of drought.
\nNumber of days without precipitation in individual years 1961–2015.
Number of days during rainless periods with duration of 9 days or more.
As an example, 10 longest rainless periods with date of its occurrence, theoretical probability and periodicity of incidence is indicated in Table 3 for every selection series (year, s0, VP, s0 and VP, s2). The absolutely longest continuous period without rainfall for s0 selection (year, VP) had 35 days and occurred between 25.9.1962 and 29.10.1962. Maximal continuous rainless event extends to 53 days in case of s2 selection for the vegetal period. This event occurred in time interval 19.7.1967–9.9.1967. Precipitation amount was only 1.6 mm in this time interval. This time interval can be considered as the rainless period. From Table 3 results that the most important rainless periods predominantly occurred in 1960s and after the year 2000.
\nTen longest rainless periods in every selection series, their theoretical probability of incidence and periodicity.
Extremely dry year 2015 does not appear in s0 selection, however, it was identified in s2 selection. 35 day continuous period during which was total precipitation amount of 2.5 mm occurred. This year was unique in that 91 rainless days between 28.5.2015 and 2.9.2015 were associated in 4 seasons by 18, 22, 16 and 35 days in selection s2.
\nDuring the verification of the model GLOBAL the assessment of the weekly values of integral soil water content to the depth of 0.8 m expressed in millimetres of water column was conducted. The selection of the layer for the calculation depended on the depth to which the soil water storage was monitored in field. The results of the simulation and the measurements are shown in Figure 6. The diagram shows a very high concordance between the calculated and the measured values which is proven by the results in Figure 7. It is a quantile-quantile plot which shows the linear trend and the correlation coefficient between the measured and the modelled values. The R-squared statistic indicates that the model as fitted explains 86.37% of the variability in measurement. The correlation coefficient equals 0.93, indicating a relatively strong relationship between the variables. Other basic characteristics of the descriptive statistics are listed in Table 4.
\nComparison between the monitored and the modelled soil water storage up to the depth of 0.80 m during the growing season of 2007.
Representation of the linear dependence via the correlation coefficients between the measured and the modelled daily values of integral soil water content to the depth of 0.8 m in Milhostov.
Statistics | \nMeasurement | \nSimulation | \n
---|---|---|
Count | \n22 | \n22 | \n
Average | \n202.12 | \n204.43 | \n
Standard deviation | \n37.89 | \n47.84 | \n
Variance | \n1435.57 | \n2288.79 | \n
Coefficient of variation | \n18.75% | \n23.40% | \n
Minimum | \n153.32 | \n157.09 | \n
Maximum | \n287.38 | \n303.43 | \n
Range | \n134.07 | \n146.34 | \n
Standard skewness | \n1.455 | \n1.877 | \n
Standard kurtosis | \n−0.284 | \n−0.584 | \n
Summary statistics.
The results show that, especially in terms of moisture, the model GLOBAL \n
Null hypothesis | \nMean measurement = Mean simulation | \n
Alternative hypothesis | \nMean measurement ≠ Mean simulation | \n
t-statistic = −0.177316 | \nTwo-sided P-value = 0.860112 | \n
Conclusion | \nDo not reject the null hypothesis for alpha = 0.05 | \n
Null hypothesis | \nSigma measurement = Sigma simulation | \n
Alternative hypothesis | \nSigma measurement ≠ Sigma simulation | \n
F-statistic = 0.627217 | \nTwo-sided P-value = 0.293035 | \n
Conclusion | \nDo not reject the null hypothesis for alpha = 0.05 | \n
Results of the t-test and F-test.
Figure 8 shows the contour lines representing the volume moisture and it was made based on the moisture values monitored up to the depth of 0.8 m by 0.10 m thick layers in Milhostov during the growing season of 2007. The picture shows that the whole profile was dry to the subsoil layers.
\nContour lines of the volumetric moisture up to 0.80 m during the growing season of 2007.
Table 6 lists the basic characteristics of the descriptive statistics applied to the following: seasonal, monthly and daily totals of ET0, D, P; average soil water storage during the growing season to the depth of 1.0 m and the mean location of GWL under the surface during the growing season. Table 6 shows that the long-term mean evaporation during the growing season in the form of ETa is 315.17 mm (59.4% of ET0) while evaporation ET0 is 530.63 mm. This leads to the long-term evaporation deficiency of 215.45 mm, which is 40.6% of ET0. Long-term mean rainfall total during the growing season is 70.0% (371.44 mm) of ET0. Long-term mean monthly total of ETa is 88.44 mm. Long-term mean daily total of ETa is 1.73 mm while ET0 is 2.91 mm. Mean depth of the GWL under the surface is 135.09 cm. Table 6 shows statistical characteristics of the seasonal, monthly and daily totals of ET0, ETa, D, P; mean soil water storage during the growing season to the depth of 1.0 m and mean location of the GWL under the surface during the growing season.
\nStatistic | \nVO | \nMonth | \nDay | \n|||||||
---|---|---|---|---|---|---|---|---|---|---|
ET0 | \nETa | \nD | \nWS | \nP | \nGWL | \nET0 | \nETa | \nET0 | \nETa | \n|
[mm] | \n[cm] | \n[mm] | \n[mm] | \n|||||||
Mean | \n530.6 | \n315.2 | \n215.5 | \n278.4 | \n371.4 | \n135.1 | \n88.4 | \n52.5 | \n2.9 | \n1.7 | \n
St. er. | \n12.0 | \n11.7 | \n16.1 | \n5.5 | \n12.7 | \n3.6 | \n1.5 | \n1.3 | \n0.0 | \n0.0 | \n
Median | \n533.5 | \n290.7 | \n209.0 | \n270.2 | \n360.9 | \n136.2 | \n87.3 | \n51.1 | \n2.8 | \n1.6 | \n
St. dev. | \n81.1 | \n79.5 | \n109.4 | \n37.2 | \n85.9 | \n24.1 | \n25.1 | \n21.9 | \n1.4 | \n1.1 | \n
S. var. | \n6580.3 | \n6316.8 | \n11958.3 | \n1384.9 | \n7373.9 | \n580.3 | \n631.8 | \n477.5 | \n1.9 | \n1.1 | \n
Kurtosis | \n0.5 | \n0.1 | \n1.3 | \n1.2 | \n2.03 | \n3.8 | \n−0.3 | \n0.5 | \n0.1 | \n0.3 | \n
Skew. | \n−0.2 | \n0.7 | \n0.8 | \n0.9 | \n0.93 | \n0.2 | \n0.3 | \n0.5 | \n0.3 | \n0.6 | \n
Range | \n409.9 | \n370.2 | \n556.4 | \n187.0 | \n444.4 | \n159.6 | \n131.9 | \n121.3 | \n9.3 | \n6.0 | \n
Min | \n302.7 | \n156.1 | \n0.2 | \n213.6 | \n226.8 | \n61.1 | \n28.3 | \n3.3 | \n0.0 | \n0.0 | \n
Max | \n712.6 | \n526.2 | \n556.6 | \n400.6 | \n671.2 | \n220.7 | \n160.2 | \n124.5 | \n9.3 | \n6.0 | \n
Count | \n46 | \n46 | \n46 | \n46 | \n46 | \n46 | \n282 | \n282 | \n8235 | \n8235 | \n
Statistical characteristics of the seasonal, monthly and daily totals of ET0, ETa, D, WS, P and GWL.
Figure 9 shows the long-term mean values of the monthly total of ET0, ETa and D. The results indicate that the highest long-term mean monthly total of evaporation deficiency is in July and August. It is caused by the fact that the mean monthly soil water storage gradually diminishes during the growing season until July when it stops at the minimum value. To the contrary, ETa increases and reaches the top value in July. The maximum value of long-term mean total of ETa occurs in June when soil profile contains enough water. From June until the end of the growing season ETa continuously drops.
\nLong-term mean monthly totals of ET0, ETa and D.
Figure 10 shows the development of D, ET0, ETa, P totals, mean soil water storage to the depth of 1 m (WS), mean temperatures (T) and mean GWL during the growing seasons between 1970 and 2015.
\nEvapotranspiration deficiency and the water regime elements during the growing seasons 1970–2015.
It is obvious that during the examined period, the difference between ET0 and ETa raised. Evaporation deficiency ‘D’ therefore increases. Variability has also grown during the last 15 years. As for standard deviation ‘D’, from 1970 to 1985 it was 63 mm, from 1986 to 2000 it was 69 mm and during 2001–2015 it reached 158 mm. The variability has raised in all examined parameters during the last 15 years (e.g. WS raised by 50%). With the exception of evaporation, the trends are balanced.
\nTable 7 correlates the examined parameters. The most significant and closest relation is between soil water storage and D (Figure 11) and GWL which are inversely proportional. On the other hand, water storage in the root zone of a soil profile statistically depends on GWL and ETa.
\nParameters | \nP [mm] | \nET0 [mm] | \nETa [mm] | \nT [°C] | \nGWL [cm] | \nWS [mm] | \nD [mm] | \n
---|---|---|---|---|---|---|---|
P [mm] | \n1.0 | \n\n | \n | \n | \n | \n | \n |
ET0 [mm] | \n−0.3 | \n1.0 | \n\n | \n | \n | \n | \n |
ETa [mm] | \n0.6 | \n0.1 | \n1.0 | \n\n | \n | \n | \n |
T [°C] | \n−0.2 | \n0.6 | \n0.0 | \n1.0 | \n\n | \n | \n |
GWL [cm] | \n0.6 | \n−0.3 | \n0.8 | \n−0.2 | \n1.0 | \n\n | \n |
WS [mm] | \n0.6 | \n−0.2 | \n0.9 | \n−0.1 | \n0.9 | \n1.0 | \n\n |
D [mm] | \n−0.7 | \n0.7 | \n−0.7 | \n0.4 | \n−0.8 | \n−0.8 | \n1.0 | \n
Correlation table of the examined parameters.
P—precipitation, ET0—potential evapotranspiration, ETa—actual evapotranspiration deficiency, T—mean temperatures, GWL—groundwater level, WS—water storage, D—evaporation.
Graphical representation of the exponential dependence between evapotranspiration deficiency (D) and water storage (WS) in soil to the depth of 1 m; R = 0.8.
The processes can be explained by the fact that GWL is a lower boundary of the unsaturated soil zone and for the purpose of soil water regime, it is defined as the lower boundary condition. The lower boundary of the unsaturated soil zone is thus dynamic and, depending on the interaction with the groundwater, it can change in space and time. When GWL is high, groundwater can reach the soil profile. In depressed lowland areas, such as ESL, groundwater often reaches the surface of the ground and the unsaturated soil zone is lost. Due to its fluctuation in time, groundwater can reach the soil profile even when the average GWL is lower. In this way, the interaction processes influence on the soil water storage and its availability for the plant cover. It is especially observable during periods of meteorological drought. During longer periods with no rainfall, precipitation cannot cover the actual evapotranspiration ETa and the drying process begins. There is a slight retardation in time in terms of the impact of the drying process on GWL and the unsaturated soil zone. In lowland areas, first layers to be dried are the upper layers of a soil profile. Drying then spreads to the lower layers towards GWL. Time retardation lies in that groundwater supplies the unsaturated soil zone with water and thus ameliorates its availability for the root zone of the plant cover. In consequence, GWL drops and the unsaturated soil zone becomes thicker. When GWL drops under the critical (threshold) point, water transfer from GWL to the root zone ceases. Moisture conditions in the balanced layer of the root zone depend solely on rainfall and evaporation. When there is the longer period with no rainfall, water supply towards the roots stops. The upper soil horizons and subsequently the whole root zone get into the state of drought. In Figure 12 the examined years are ordered by mean soil water storage to the depth of 1 m during the growing season.
\nGrowing seasons 1970–2015 ordered by soil water storage in a soil profile to the depth of 1 m.
The scheme shows that the driest year in terms of soil water storage during a growing season was the year 2015. In consequence, water regime elements in 2015 were analysed. The results of the analysis were calculated with 1-day step and they are shown in Figure 13. Evaporation deficiency ‘D’ is 257% of the long-term mean (1971–2015). ET0 is 134% and ETa is 49% of the corresponding long-term mean. The mean temperature is relatively stable, 110% of the corresponding long-term mean. Rainfall ‘P’ formed 61% of the corresponding long-term mean and water storage in soil to the depth of 1 m ‘WS’ were 77% of the corresponding long-term mean. The results shown in Figure 13 indicate that in the other half of the growing season soil water storage dropped under the point of decreased availability.
\nEvapotranspiration deficiency and water regime elements in the extremely dry year of 2015.
This corresponds to the fact that the value of ‘D’ was high above the average. Groundwater continuously drops during the whole growing season. Figure 13 also shows the development and correlation between ET0, ETa and D.
\nPrecipitation amount and temporal distribution of the rainfall is important for water refilling of the environment for balanced periods. Drying of soil profile occurs during long rainless periods. Meteorological drought and subsequently soil drought occurs in the case of the sufficiently long rainless period. Therefore it is necessary to know size and statistical characteristics of rainless periods (RLP). The aim of the contribution is to identify important rainless periods, quantify temporal lengths, probability characteristics and trends of RLP. Climatic station of Milhostov (N 48°39,786′; E 21°43,298′) was chosen for selection of statistically important rainless periods. Station represents wider area of lowland. Daily precipitation amounts of the 1961–2015 period was examined for the station. 20,080 daily precipitation amounts (including zero) were analysed for the period. Length of RLP was identified in two ways. Periods with zero daily precipitation amounts were considered in first selection (s0). Daily precipitation amounts lower than 2 mm were considered as zero in second selection (s2). The absolutely longest continuous period without rainfall for s0 selection (year, VP) had 35 days and occurred between 25.9.1962 and 29.10.1962. Maximal continuous rainless event extends to 53 days in case of s2 selection for the vegetal period. This event occurred in time interval 19.7.1967–9.9.1967. Number of rainy and rainless days is for individual years and their vegetal periods balanced in the long term view. Only duration of rainless events of non-vegetal periods changes in examined periods. Growth of time length of rainless days duration was identified in non-vegetal half-year.
\nThe study analysed the development of ET0, ETa, D, WS, P, GWL location and T during the growing seasons during the years 1970–2015 on the basis of the measured data and the data gained via numerical simulation. The interaction processes in the root zone of a soil profile during the creation of soil water regime were quantified. The quantification is crucial for understanding the processes occurring during drought creation, duration and termination. It has been demonstrated that soil water storage depends heavily on evaporation, i.e. actual evapotranspiration ETa. Subsequently, actual evapotranspiration influences on evapotranspiration deficiency ‘D’ and on the location of GWL. For that reason, evapotranspiration deficiency ‘D’ can be considered an indicator of the drying of a soil profile. Drying starts when water inflow towards plant roots is reduced. During the state of evapotranspiration deficiency, groundwater supplies the root zone of a soil profile with water. When there is the long period with no rainfall, water transfer from GWL towards plant roots ceases. Moisture conditions in the balanced layer of the root zone depend solely on rainfall and evaporation. The processes are demonstrated on the driest growing season of 2015. From the point of view of soil water storage to the depth of 1 m this season was absolutely driest growing season in the examined period from 1970 to 2015.
\nThis work was created thanks to financial support of VEGA project 2/0062/16.
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conducts his research at the Hamidiye Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Division of Biochemistry, University of Health Sciences, Turkey. He is also a faculty member in the Molecular Oncology Program. He obtained his MSc and Ph.D. at Oregon State University and Texas Tech University, respectively. He pursued his postdoctoral studies at Rutgers University Medical School and the National Institutes of Health (NIH/NIDDK), USA. His research focuses on biochemistry, biophysics, genetics, molecular biology, and molecular medicine with specialization in the fields of drug design, protein structure-function, protein folding, prions, microRNA, pseudogenes, molecular cancer, epigenetics, metabolites, proteomics, genomics, protein expression, and characterization by spectroscopic and calorimetric methods.",institutionString:"University of Health Sciences",institution:null},{id:"180528",title:"Dr.",name:"Hiroyuki",middleName:null,surname:"Kagechika",slug:"hiroyuki-kagechika",fullName:"Hiroyuki Kagechika",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180528/images/system/180528.jpg",biography:"Hiroyuki Kagechika received his bachelor’s degree and Ph.D. in Pharmaceutical Sciences from the University of Tokyo, Japan, where he served as an associate professor until 2004. He is currently a professor at the Institute of Biomaterials and Bioengineering (IBB), Tokyo Medical and Dental University (TMDU). From 2010 to 2012, he was the dean of the Graduate School of Biomedical Science. Since 2012, he has served as the vice dean of the Graduate School of Medical and Dental Sciences. He has been the director of the IBB since 2020. Dr. Kagechika’s major research interests are the medicinal chemistry of retinoids, vitamins D/K, and nuclear receptors. He has developed various compounds including a drug for acute promyelocytic leukemia.",institutionString:"Tokyo Medical and Dental University",institution:{name:"Tokyo Medical and Dental University",country:{name:"Japan"}}},{id:"40482",title:null,name:"Rizwan",middleName:null,surname:"Ahmad",slug:"rizwan-ahmad",fullName:"Rizwan Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/40482/images/system/40482.jpeg",biography:"Dr. Rizwan Ahmad is a University Professor and Coordinator, Quality and Development, College of Medicine, Imam Abdulrahman bin Faisal University, Saudi Arabia. Previously, he was Associate Professor of Human Function, Oman Medical College, Oman, and SBS University, Dehradun. Dr. Ahmad completed his education at Aligarh Muslim University, Aligarh. He has published several articles in peer-reviewed journals, chapters, and edited books. His area of specialization is free radical biochemistry and autoimmune diseases.",institutionString:"Imam Abdulrahman Bin Faisal University",institution:{name:"Imam Abdulrahman Bin Faisal University",country:{name:"Saudi Arabia"}}},{id:"41865",title:"Prof.",name:"Farid A.",middleName:null,surname:"Badria",slug:"farid-a.-badria",fullName:"Farid A. Badria",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/41865/images/system/41865.jpg",biography:"Farid A. Badria, Ph.D., is the recipient of several awards, including The World Academy of Sciences (TWAS) Prize for Public Understanding of Science; the World Intellectual Property Organization (WIPO) Gold Medal for best invention; Outstanding Arab Scholar, Kuwait; and the Khwarizmi International Award, Iran. He has 250 publications, 12 books, 20 patents, and several marketed pharmaceutical products to his credit. He continues to lead research projects on developing new therapies for liver, skin disorders, and cancer. Dr. Badria was listed among the world’s top 2% of scientists in medicinal and biomolecular chemistry in 2019 and 2020. He is a member of the Arab Development Fund, Kuwait; International Cell Research Organization–United Nations Educational, Scientific and Cultural Organization (ICRO–UNESCO), Chile; and UNESCO Biotechnology France",institutionString:"Mansoura University",institution:{name:"Mansoura University",country:{name:"Egypt"}}},{id:"329385",title:"Dr.",name:"Rajesh K.",middleName:"Kumar",surname:"Singh",slug:"rajesh-k.-singh",fullName:"Rajesh K. Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329385/images/system/329385.png",biography:"Dr. Singh received a BPharm (2003) and MPharm (2005) from Panjab University, Chandigarh, India, and a Ph.D. (2013) from Punjab Technical University (PTU), Jalandhar, India. He has more than sixteen years of teaching experience and has supervised numerous postgraduate and Ph.D. students. He has to his credit more than seventy papers in SCI- and SCOPUS-indexed journals, fifty-five conference proceedings, four books, six Best Paper Awards, and five projects from different government agencies. He is currently an editorial board member of eight international journals and a reviewer for more than fifty scientific journals. He received Top Reviewer and Excellent Peer Reviewer Awards from Publons in 2016 and 2017, respectively. He is also on the panel of The International Reviewer for reviewing research proposals for grants from the Royal Society. He also serves as a Publons Academy mentor and Bentham brand ambassador.",institutionString:"Punjab Technical University",institution:{name:"Punjab Technical University",country:{name:"India"}}},{id:"142388",title:"Dr.",name:"Thiago",middleName:"Gomes",surname:"Gomes Heck",slug:"thiago-gomes-heck",fullName:"Thiago Gomes Heck",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/142388/images/7259_n.jpg",biography:null,institutionString:null,institution:{name:"Universidade Regional do Noroeste do Estado do Rio Grande do Sul",country:{name:"Brazil"}}},{id:"336273",title:"Assistant Prof.",name:"Janja",middleName:null,surname:"Zupan",slug:"janja-zupan",fullName:"Janja Zupan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/336273/images/14853_n.jpeg",biography:"Janja Zupan graduated in 2005 at the Department of Clinical Biochemistry (superviser prof. dr. Janja Marc) in the field of genetics of osteoporosis. Since November 2009 she is working as a Teaching Assistant at the Faculty of Pharmacy, Department of Clinical Biochemistry. In 2011 she completed part of her research and PhD work at Institute of Genetics and Molecular Medicine, University of Edinburgh. She finished her PhD entitled The influence of the proinflammatory cytokines on the RANK/RANKL/OPG in bone tissue of osteoporotic and osteoarthritic patients in 2012. From 2014-2016 she worked at the Institute of Biomedical Sciences, University of Aberdeen as a postdoctoral research fellow on UK Arthritis research project where she gained knowledge in mesenchymal stem cells and regenerative medicine. She returned back to University of Ljubljana, Faculty of Pharmacy in 2016. She is currently leading project entitled Mesenchymal stem cells-the keepers of tissue endogenous regenerative capacity facing up to aging of the musculoskeletal system funded by Slovenian Research Agency.",institutionString:null,institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"357453",title:"Dr.",name:"Radheshyam",middleName:null,surname:"Maurya",slug:"radheshyam-maurya",fullName:"Radheshyam Maurya",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/357453/images/16535_n.jpg",biography:null,institutionString:null,institution:{name:"University of Hyderabad",country:{name:"India"}}},{id:"311457",title:"Dr.",name:"Júlia",middleName:null,surname:"Scherer Santos",slug:"julia-scherer-santos",fullName:"Júlia Scherer Santos",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311457/images/system/311457.jpg",biography:"Dr. Júlia Scherer Santos works in the areas of cosmetology, nanotechnology, pharmaceutical technology, beauty, and aesthetics. Dr. Santos also has experience as a professor of graduate courses. Graduated in Pharmacy, specialization in Cosmetology and Cosmeceuticals applied to aesthetics, specialization in Aesthetic and Cosmetic Health, and a doctorate in Pharmaceutical Nanotechnology. Teaching experience in Pharmacy and Aesthetics and Cosmetics courses. She works mainly on the following subjects: nanotechnology, cosmetology, pharmaceutical technology, aesthetics.",institutionString:"Universidade Federal de Juiz de Fora",institution:{name:"Universidade Federal de Juiz de Fora",country:{name:"Brazil"}}},{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",slug:"abdulsamed-kukurt",fullName:"Abdulsamed Kükürt",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRNVJQA4/Profile_Picture_2022-03-07T13:23:04.png",biography:"Dr. Kükürt graduated from Uludağ University in Turkey. He started his academic career as a Research Assistant in the Department of Biochemistry at Kafkas University. In 2019, he completed his Ph.D. program in the Department of Biochemistry at the Institute of Health Sciences. He is currently working at the Department of Biochemistry, Kafkas University. He has 27 published research articles in academic journals, 11 book chapters, and 37 papers. He took part in 10 academic projects. He served as a reviewer for many articles. He still serves as a member of the review board in many academic journals.",institutionString:null,institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"178366",title:"Associate Prof.",name:"Volkan",middleName:null,surname:"Gelen",slug:"volkan-gelen",fullName:"Volkan Gelen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178366/images/system/178366.jpg",biography:"Volkan Gelen is a Physiology specialist who received his veterinary degree from Kafkas University in 2011. Between 2011-2015, he worked as an assistant at Atatürk University, Faculty of Veterinary Medicine, Department of Physiology. In 2016, he joined Kafkas University, Faculty of Veterinary Medicine, Department of Physiology as an assistant professor. Dr. Gelen has been engaged in various academic activities at Kafkas University since 2016. There he completed 5 projects and has 3 ongoing projects. He has 60 articles published in scientific journals and 20 poster presentations in scientific congresses. His research interests include physiology, endocrine system, cancer, diabetes, cardiovascular system diseases, and isolated organ bath system studies.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"418963",title:"Dr.",name:"Augustine Ododo",middleName:"Augustine",surname:"Osagie",slug:"augustine-ododo-osagie",fullName:"Augustine Ododo Osagie",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/418963/images/16900_n.jpg",biography:"Born into the family of Osagie, a prince of the Benin Kingdom. I am currently an academic in the Department of Medical Biochemistry, University of Benin. Part of the duties are to teach undergraduate students and conduct academic research.",institutionString:null,institution:{name:"University of Benin",country:{name:"Nigeria"}}},{id:"192992",title:"Prof.",name:"Shagufta",middleName:null,surname:"Perveen",slug:"shagufta-perveen",fullName:"Shagufta Perveen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192992/images/system/192992.png",biography:"Prof. Shagufta Perveen is a Distinguish Professor in the Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Dr. Perveen has acted as the principal investigator of major research projects funded by the research unit of King Saud University. She has more than ninety original research papers in peer-reviewed journals of international repute to her credit. She is a fellow member of the Royal Society of Chemistry UK and the American Chemical Society of the United States.",institutionString:"King Saud University",institution:{name:"King Saud University",country:{name:"Saudi Arabia"}}},{id:"49848",title:"Dr.",name:"Wen-Long",middleName:null,surname:"Hu",slug:"wen-long-hu",fullName:"Wen-Long Hu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49848/images/system/49848.jpg",biography:"Wen-Long Hu is Chief of the Division of Acupuncture, Department of Chinese Medicine at Kaohsiung Chang Gung Memorial Hospital, as well as an adjunct associate professor at Fooyin University and Kaohsiung Medical University. Wen-Long is President of Taiwan Traditional Chinese Medicine Medical Association. He has 28 years of experience in clinical practice in laser acupuncture therapy and 34 years in acupuncture. He is an invited speaker for lectures and workshops in laser acupuncture at many symposiums held by medical associations. He owns the patent for herbal preparation and producing, and for the supercritical fluid-treated needle. Dr. Hu has published three books, 12 book chapters, and more than 30 papers in reputed journals, besides serving as an editorial board member of repute.",institutionString:"Kaohsiung Chang Gung Memorial Hospital",institution:{name:"Kaohsiung Chang Gung Memorial Hospital",country:{name:"Taiwan"}}},{id:"298472",title:"Prof.",name:"Andrey V.",middleName:null,surname:"Grechko",slug:"andrey-v.-grechko",fullName:"Andrey V. Grechko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/298472/images/system/298472.png",biography:"Andrey Vyacheslavovich Grechko, Ph.D., Professor, is a Corresponding Member of the Russian Academy of Sciences. He graduated from the Semashko Moscow Medical Institute (Semashko National Research Institute of Public Health) with a degree in Medicine (1998), the Clinical Department of Dermatovenerology (2000), and received a second higher education in Psychology (2009). Professor A.V. Grechko held the position of Сhief Physician of the Central Clinical Hospital in Moscow. He worked as a professor at the faculty and was engaged in scientific research at the Medical University. Starting in 2013, he has been the initiator of the creation of the Federal Scientific and Clinical Center for Intensive Care and Rehabilitology, Moscow, Russian Federation, where he also serves as Director since 2015. He has many years of experience in research and teaching in various fields of medicine, is an author/co-author of more than 200 scientific publications, 13 patents, 15 medical books/chapters, including Chapter in Book «Metabolomics», IntechOpen, 2020 «Metabolomic Discovery of Microbiota Dysfunction as the Cause of Pathology».",institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"199461",title:"Prof.",name:"Natalia V.",middleName:null,surname:"Beloborodova",slug:"natalia-v.-beloborodova",fullName:"Natalia V. Beloborodova",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/199461/images/system/199461.jpg",biography:'Natalia Vladimirovna Beloborodova was educated at the Pirogov Russian National Research Medical University, with a degree in pediatrics in 1980, a Ph.D. in 1987, and a specialization in Clinical Microbiology from First Moscow State Medical University in 2004. She has been a Professor since 1996. Currently, she is the Head of the Laboratory of Metabolism, a division of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russian Federation. N.V. Beloborodova has many years of clinical experience in the field of intensive care and surgery. She studies infectious complications and sepsis. She initiated a series of interdisciplinary clinical and experimental studies based on the concept of integrating human metabolism and its microbiota. Her scientific achievements are widely known: she is the recipient of the Marie E. Coates Award \\"Best lecturer-scientist\\" Gustafsson Fund, Karolinska Institutes, Stockholm, Sweden, and the International Sepsis Forum Award, Pasteur Institute, Paris, France (2014), etc. Professor N.V. Beloborodova wrote 210 papers, five books, 10 chapters and has edited four books.',institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"354260",title:"Ph.D.",name:"Tércio Elyan",middleName:"Azevedo",surname:"Azevedo Martins",slug:"tercio-elyan-azevedo-martins",fullName:"Tércio Elyan Azevedo Martins",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/354260/images/16241_n.jpg",biography:"Graduated in Pharmacy from the Federal University of Ceará with the modality in Industrial Pharmacy, Specialist in Production and Control of Medicines from the University of São Paulo (USP), Master in Pharmaceuticals and Medicines from the University of São Paulo (USP) and Doctor of Science in the program of Pharmaceuticals and Medicines by the University of São Paulo. Professor at Universidade Paulista (UNIP) in the areas of chemistry, cosmetology and trichology. Assistant Coordinator of the Higher Course in Aesthetic and Cosmetic Technology at Universidade Paulista Campus Chácara Santo Antônio. Experience in the Pharmacy area, with emphasis on Pharmacotechnics, Pharmaceutical Technology, Research and Development of Cosmetics, acting mainly on topics such as cosmetology, antioxidant activity, aesthetics, photoprotection, cyclodextrin and thermal analysis.",institutionString:null,institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"334285",title:"Ph.D. Student",name:"Sameer",middleName:"Kumar",surname:"Jagirdar",slug:"sameer-jagirdar",fullName:"Sameer Jagirdar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/334285/images/14691_n.jpg",biography:"I\\'m a graduate student at the center for biosystems science and engineering at the Indian Institute of Science, Bangalore, India. I am interested in studying host-pathogen interactions at the biomaterial interface.",institutionString:null,institution:{name:"Indian Institute of Science Bangalore",country:{name:"India"}}},{id:"329795",title:"Dr.",name:"Mohd Aftab",middleName:"Aftab",surname:"Siddiqui",slug:"mohd-aftab-siddiqui",fullName:"Mohd Aftab Siddiqui",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329795/images/15648_n.jpg",biography:"Dr. Mohd Aftab Siddiqui is currently working as Assistant Professor in the Faculty of Pharmacy, Integral University, Lucknow for the last 6 years. He has completed his Doctor in Philosophy (Pharmacology) in 2020 from Integral University, Lucknow. He completed his Bachelor in Pharmacy in 2013 and Master in Pharmacy (Pharmacology) in 2015 from Integral University, Lucknow. He is the gold medalist in Bachelor and Master degree. He qualified GPAT -2013, GPAT -2014, and GPAT 2015. His area of research is Pharmacological screening of herbal drugs/ natural products in liver and cardiac diseases. He has guided many M. Pharm. research projects. He has many national and international publications.",institutionString:"Integral University",institution:null},{id:"255360",title:"Dr.",name:"Usama",middleName:null,surname:"Ahmad",slug:"usama-ahmad",fullName:"Usama Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255360/images/system/255360.png",biography:"Dr. Usama Ahmad holds a specialization in Pharmaceutics from Amity University, Lucknow, India. He received his Ph.D. degree from Integral University. Currently, he’s working as an Assistant Professor of Pharmaceutics in the Faculty of Pharmacy, Integral University. From 2013 to 2014 he worked on a research project funded by SERB-DST, Government of India. He has a rich publication record with more than 32 original articles published in reputed journals, 3 edited books, 5 book chapters, and a number of scientific articles published in ‘Ingredients South Asia Magazine’ and ‘QualPharma Magazine’. He is a member of the American Association for Cancer Research, International Association for the Study of Lung Cancer, and the British Society for Nanomedicine. Dr. Ahmad’s research focus is on the development of nanoformulations to facilitate the delivery of drugs that aim to provide practical solutions to current healthcare problems.",institutionString:"Integral University",institution:{name:"Integral University",country:{name:"India"}}},{id:"30568",title:"Prof.",name:"Madhu",middleName:null,surname:"Khullar",slug:"madhu-khullar",fullName:"Madhu Khullar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/30568/images/system/30568.jpg",biography:"Dr. Madhu Khullar is a Professor of Experimental Medicine and Biotechnology at the Post Graduate Institute of Medical Education and Research, Chandigarh, India. She completed her Post Doctorate in hypertension research at the Henry Ford Hospital, Detroit, USA in 1985. She is an editor and reviewer of several international journals, and a fellow and member of several cardiovascular research societies. Dr. Khullar has a keen research interest in genetics of hypertension, and is currently studying pharmacogenetics of hypertension.",institutionString:"Post Graduate Institute of Medical Education and Research",institution:{name:"Post Graduate Institute of Medical Education and Research",country:{name:"India"}}},{id:"223233",title:"Prof.",name:"Xianquan",middleName:null,surname:"Zhan",slug:"xianquan-zhan",fullName:"Xianquan Zhan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/223233/images/system/223233.png",biography:"Xianquan Zhan received his MD and Ph.D. in Preventive Medicine at West China University of Medical Sciences. He received his post-doctoral training in oncology and cancer proteomics at the Central South University, China, and the University of Tennessee Health Science Center (UTHSC), USA. He worked at UTHSC and the Cleveland Clinic in 2001–2012 and achieved the rank of associate professor at UTHSC. Currently, he is a full professor at Central South University and Shandong First Medical University, and an advisor to MS/PhD students and postdoctoral fellows. He is also a fellow of the Royal Society of Medicine and European Association for Predictive Preventive Personalized Medicine (EPMA), a national representative of EPMA, and a member of the American Society of Clinical Oncology (ASCO) and the American Association for the Advancement of Sciences (AAAS). He is also the editor in chief of International Journal of Chronic Diseases & Therapy, an associate editor of EPMA Journal, Frontiers in Endocrinology, and BMC Medical Genomics, and a guest editor of Mass Spectrometry Reviews, Frontiers in Endocrinology, EPMA Journal, and Oxidative Medicine and Cellular Longevity. He has published more than 148 articles, 28 book chapters, 6 books, and 2 US patents in the field of clinical proteomics and biomarkers.",institutionString:"Shandong First Medical University",institution:{name:"Affiliated Hospital of Shandong Academy of Medical Sciences",country:{name:"China"}}},{id:"297507",title:"Dr.",name:"Charles",middleName:"Elias",surname:"Assmann",slug:"charles-assmann",fullName:"Charles Assmann",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/297507/images/system/297507.jpg",biography:"Charles Elias Assmann is a biologist from Federal University of Santa Maria (UFSM, Brazil), who spent some time abroad at the Ludwig-Maximilians-Universität München (LMU, Germany). He has Masters Degree in Biochemistry (UFSM), and is currently a PhD student at Biochemistry at the Department of Biochemistry and Molecular Biology of the UFSM. His areas of expertise include: Biochemistry, Molecular Biology, Enzymology, Genetics and Toxicology. He is currently working on the following subjects: Aluminium toxicity, Neuroinflammation, Oxidative stress and Purinergic system. Since 2011 he has presented more than 80 abstracts in scientific proceedings of national and international meetings. Since 2014, he has published more than 20 peer reviewed papers (including 4 reviews, 3 in Portuguese) and 2 book chapters. He has also been a reviewer of international journals and ad hoc reviewer of scientific committees from Brazilian Universities.",institutionString:"Universidade Federal de Santa Maria",institution:{name:"Universidade Federal de Santa Maria",country:{name:"Brazil"}}},{id:"217850",title:"Dr.",name:"Margarete Dulce",middleName:null,surname:"Bagatini",slug:"margarete-dulce-bagatini",fullName:"Margarete Dulce Bagatini",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/217850/images/system/217850.jpeg",biography:"Dr. Margarete Dulce Bagatini is an associate professor at the Federal University of Fronteira Sul/Brazil. She has a degree in Pharmacy and a PhD in Biological Sciences: Toxicological Biochemistry. She is a member of the UFFS Research Advisory Committee\nand a member of the Biovitta Research Institute. She is currently:\nthe leader of the research group: Biological and Clinical Studies\nin Human Pathologies, professor of postgraduate program in\nBiochemistry at UFSC and postgraduate program in Science and Food Technology at\nUFFS. She has experience in the area of pharmacy and clinical analysis, acting mainly\non the following topics: oxidative stress, the purinergic system and human pathologies, being a reviewer of several international journals and books.",institutionString:"Universidade Federal da Fronteira Sul",institution:{name:"Universidade Federal da Fronteira Sul",country:{name:"Brazil"}}},{id:"226275",title:"Ph.D.",name:"Metin",middleName:null,surname:"Budak",slug:"metin-budak",fullName:"Metin Budak",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/226275/images/system/226275.jfif",biography:"Metin Budak, MSc, PhD is an Assistant Professor at Trakya University, Faculty of Medicine. He has been Head of the Molecular Research Lab at Prof. Mirko Tos Ear and Hearing Research Center since 2018. His specializations are biophysics, epigenetics, genetics, and methylation mechanisms. He has published around 25 peer-reviewed papers, 2 book chapters, and 28 abstracts. He is a member of the Clinical Research Ethics Committee and Quantification and Consideration Committee of Medicine Faculty. His research area is the role of methylation during gene transcription, chromatin packages DNA within the cell and DNA repair, replication, recombination, and gene transcription. His research focuses on how the cell overcomes chromatin structure and methylation to allow access to the underlying DNA and enable normal cellular function.",institutionString:"Trakya University",institution:{name:"Trakya University",country:{name:"Turkey"}}},{id:"243049",title:"Dr.",name:"Anca",middleName:null,surname:"Pantea Stoian",slug:"anca-pantea-stoian",fullName:"Anca Pantea Stoian",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243049/images/system/243049.jpg",biography:"Anca Pantea Stoian is a specialist in diabetes, nutrition, and metabolic diseases as well as health food hygiene. She also has competency in general ultrasonography.\n\nShe is an associate professor in the Diabetes, Nutrition and Metabolic Diseases Department, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. She has been chief of the Hygiene Department, Faculty of Dentistry, at the same university since 2019. Her interests include micro and macrovascular complications in diabetes and new therapies. Her research activities focus on nutritional intervention in chronic pathology, as well as cardio-renal-metabolic risk assessment, and diabetes in cancer. She is currently engaged in developing new therapies and technological tools for screening, prevention, and patient education in diabetes. \n\nShe is a member of the European Association for the Study of Diabetes, Cardiometabolic Academy, CEDA, Romanian Society of Diabetes, Nutrition and Metabolic Diseases, Romanian Diabetes Federation, and Association for Renal Metabolic and Nutrition studies. She has authored or co-authored 160 papers in national and international peer-reviewed journals.",institutionString:null,institution:{name:"Carol Davila University of Medicine and Pharmacy",country:{name:"Romania"}}},{id:"279792",title:"Dr.",name:"João",middleName:null,surname:"Cotas",slug:"joao-cotas",fullName:"João Cotas",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/279792/images/system/279792.jpg",biography:"Graduate and master in Biology from the University of Coimbra.\n\nI am a research fellow at the Macroalgae Laboratory Unit, in the MARE-UC – Marine and Environmental Sciences Centre of the University of Coimbra. My principal function is the collection, extraction and purification of macroalgae compounds, chemical and bioactive characterization of the compounds and algae extracts and development of new methodologies in marine biotechnology area. \nI am associated in two projects: one consists on discovery of natural compounds for oncobiology. The other project is the about the natural compounds/products for agricultural area.\n\nPublications:\nCotas, J.; Figueirinha, A.; Pereira, L.; Batista, T. 2018. An analysis of the effects of salinity on Fucus ceranoides (Ochrophyta, Phaeophyceae), in the Mondego River (Portugal). Journal of Oceanology and Limnology. in press. DOI: 10.1007/s00343-019-8111-3",institutionString:"Faculty of Sciences and Technology of University of Coimbra",institution:null},{id:"279788",title:"Dr.",name:"Leonel",middleName:null,surname:"Pereira",slug:"leonel-pereira",fullName:"Leonel Pereira",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/279788/images/system/279788.jpg",biography:"Leonel Pereira has an undergraduate degree in Biology, a Ph.D. in Biology (specialty in Cell Biology), and a Habilitation degree in Biosciences (specialization in Biotechnology) from the Faculty of Science and Technology, University of Coimbra, Portugal, where he is currently a professor. In addition to teaching at this university, he is an integrated researcher at the Marine and Environmental Sciences Center (MARE), Portugal. His interests include marine biodiversity (algae), marine biotechnology (algae bioactive compounds), and marine ecology (environmental assessment). Since 2008, he has been the author and editor of the electronic publication MACOI – Portuguese Seaweeds Website (www.seaweeds.uc.pt). He is also a member of the editorial boards of several scientific journals. Dr. Pereira has edited or authored more than 20 books, 100 journal articles, and 45 book chapters. He has given more than 100 lectures and oral communications at various national and international scientific events. He is the coordinator of several national and international research projects. In 1998, he received the Francisco de Holanda Award (Honorable Mention) and, more recently, the Mar Rei D. Carlos award (18th edition). He is also a winner of the 2016 CHOICE Award for an outstanding academic title for his book Edible Seaweeds of the World. In 2020, Dr. Pereira received an Honorable Mention for the Impact of International Publications from the Web of Science",institutionString:"University of Coimbra",institution:{name:"University of Coimbra",country:{name:"Portugal"}}},{id:"61946",title:"Dr.",name:"Carol",middleName:null,surname:"Bernstein",slug:"carol-bernstein",fullName:"Carol Bernstein",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/61946/images/system/61946.jpg",biography:"Carol Bernstein received her PhD in Genetics from the University of California (Davis). She was a faculty member at the University of Arizona College of Medicine for 43 years, retiring in 2011. Her research interests focus on DNA damage and its underlying role in sex, aging and in the early steps of initiation and progression to cancer. In her research, she had used organisms including bacteriophage T4, Neurospora crassa, Schizosaccharomyces pombe and mice, as well as human cells and tissues. She authored or co-authored more than 140 scientific publications, including articles in major peer reviewed journals, book chapters, invited reviews and one book.",institutionString:"University of Arizona",institution:{name:"University of Arizona",country:{name:"United States of America"}}},{id:"182258",title:"Dr.",name:"Ademar",middleName:"Pereira",surname:"Serra",slug:"ademar-serra",fullName:"Ademar Serra",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/182258/images/system/182258.jpeg",biography:"Dr. Serra studied Agronomy on Universidade Federal de Mato Grosso do Sul (UFMS) (2005). He received master degree in Agronomy, Crop Science (Soil fertility and plant nutrition) (2007) by Universidade Federal da Grande Dourados (UFGD), and PhD in agronomy (Soil fertility and plant nutrition) (2011) from Universidade Federal da Grande Dourados / Escola Superior de Agricultura Luiz de Queiroz (UFGD/ESALQ-USP). Dr. Serra is currently working at Brazilian Agricultural Research Corporation (EMBRAPA). His research focus is on mineral nutrition of plants, crop science and soil science. Dr. Serra\\'s current projects are soil organic matter, soil phosphorus fractions, compositional nutrient diagnosis (CND) and isometric log ratio (ilr) transformation in compositional data analysis.",institutionString:"Brazilian Agricultural Research Corporation",institution:{name:"Brazilian Agricultural Research Corporation",country:{name:"Brazil"}}}]}},subseries:{item:{id:"28",type:"subseries",title:"Animal Reproductive Biology and Technology",keywords:"Animal Reproduction, Artificial Insemination, Embryos, Cryopreservation, Conservation, Breeding, Epigenetics",scope:"The advances of knowledge on animal reproductive biology and technologies revolutionized livestock production. Artificial insemination, for example, was the first technology applied on a large scale, initially in dairy cattle and afterward applied to other species. Nowadays, embryo production and transfer are used commercially along with other technologies to modulate epigenetic regulation. Gene editing is also emerging as an innovative tool. This topic will discuss the potential use of these techniques, novel strategies, and lines of research in progress in the fields mentioned above.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/28.jpg",hasOnlineFirst:!0,hasPublishedBooks:!0,annualVolume:11417,editor:{id:"177225",title:"Prof.",name:"Rosa Maria Lino Neto",middleName:null,surname:"Pereira",slug:"rosa-maria-lino-neto-pereira",fullName:"Rosa Maria Lino Neto Pereira",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bS9wkQAC/Profile_Picture_1624519982291",biography:"Rosa Maria Lino Neto Pereira (DVM, MsC, PhD and) is currently a researcher at the Genetic Resources and Biotechnology Unit of the National Institute of Agrarian and Veterinarian Research (INIAV, Portugal). She is the head of the Reproduction and Embryology Laboratories and was lecturer of Reproduction and Reproductive Biotechnologies at Veterinary Medicine Faculty. She has over 25 years of experience working in reproductive biology and biotechnology areas with a special emphasis on embryo and gamete cryopreservation, for research and animal genetic resources conservation, leading research projects with several peer-reviewed papers. Rosa Pereira is member of the ERFP-FAO Ex situ Working Group and of the Management Commission of the Portuguese Animal Germplasm Bank.",institutionString:"The National Institute for Agricultural and Veterinary Research. Portugal",institution:null},editorTwo:null,editorThree:null,series:{id:"13",title:"Veterinary Medicine and Science",doi:"10.5772/intechopen.73681",issn:"2632-0517"},editorialBoard:[{id:"90066",title:"Dr.",name:"Alexandre",middleName:"Rodrigues",surname:"Silva",slug:"alexandre-silva",fullName:"Alexandre Silva",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRt8pQAC/Profile_Picture_1622531020756",institutionString:null,institution:{name:"Universidade Federal Rural do Semi-Árido",institutionURL:null,country:{name:"Brazil"}}},{id:"176987",title:"Ph.D.",name:"María-José",middleName:"Carrascosa",surname:"Argente",slug:"maria-jose-argente",fullName:"María-José Argente",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bS9vOQAS/Profile_Picture_1630330499537",institutionString:null,institution:{name:"Miguel Hernandez University",institutionURL:null,country:{name:"Spain"}}},{id:"321396",title:"Prof.",name:"Muhammad Subhan",middleName:null,surname:"Qureshi",slug:"muhammad-subhan-qureshi",fullName:"Muhammad Subhan Qureshi",profilePictureURL:"https://mts.intechopen.com/storage/users/321396/images/system/321396.jpg",institutionString:null,institution:{name:"University of Agriculture",institutionURL:null,country:{name:"Pakistan"}}},{id:"183723",title:"Dr.",name:"Xiaojun",middleName:null,surname:"Liu",slug:"xiaojun-liu",fullName:"Xiaojun Liu",profilePictureURL:"https://mts.intechopen.com/storage/users/183723/images/system/183723.jpg",institutionString:null,institution:null}]},onlineFirstChapters:{},publishedBooks:{paginationCount:3,paginationItems:[{type:"book",id:"8545",title:"Animal Reproduction in Veterinary Medicine",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/8545.jpg",slug:"animal-reproduction-in-veterinary-medicine",publishedDate:"January 20th 2021",editedByType:"Edited by",bookSignature:"Faruk Aral, Rita Payan-Carreira and Miguel 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