Chemical structures of major polyphenols (flavonoids: flavanols and flavonols) in Green tea and Black tea.
This book chapter describes about the phytochemicals in tea which are significantly useful in preparing nutraceutical products. The polyphenols along with other bioactive compounds present in tea have many pharmacological properties which attribute to the development of various food products where tea constitutes as an active ingredient. This chapter also discusses the potential uses of tea and their bioactive constituents in treatment and prevention of diseases in human which infer the potentiality of developing and popularizing nutraceuticals of tea.
- therapeutic properties
Nutraceuticals are promising class of natural products that encompasses the combined terms “nutrition” and pharmaceuticals” which incorporates the characteristics of both nutritional and pharmaceutical and thus exhibit several health benefits. The food or food products having nutraceuticals properties can be used as medicine in addition to nutritional values and have been used to support proper functioning of the body, treat and prevention of diseases, and increase the life expectancy of human beings [1, 2, 3, 4]. Currently nutraceuticals have been explored in several diseases in prevention and cure such cancer, diabetics, cardiovascular, diseases etc.
The black tea is fully fermented and known for its characteristics brown liquor whereas green tea is unfermented and known for its light greenish yellow liquor. The oolong is semi-fermented and liquor characteristic lies in between the black and green tea. All the three types differ in their taste and flavor and accordingly their chemical profiling also varies.
Tea contains a variety of bioactive compounds such as polyphenols, polysaccharides, vitamins, amino acids etc. having medicinal properties which can be used as food additives in preparation of nutraceuticals [5, 6, 7, 8]. Tea is mainly chemically characterized by their polyphenolic compounds and their polymeriszed products along other bioactive compounds in minor quantities.
The polyphenols which are produced by the plant as secondary metabolites are the major constituents in tea. Other secondary metabolites present in tea are phenolic acids, purine alkaloids, tannins, flavonols and their glycosides. The polyphenols in tea are catechins (C) and their conjugated products viz. epigallocatechingallate (EGCG), epigallocatechins (EGC), epicatechingallate (ECG) and epicatechins (EC) and present in higher quantities in green tea. The other two class of polyphenols found in black tea are theaflavins (TFs) and thearubigins (TRs) which are present in significant quantities in black tea and less quantity in oolong tea. The catechin polyphenols in tea are health-promising due to their antioxidant properties and are very useful in preparation of nutraceuticals from tea [9, 10, 11, 12, 13, 14].
In addition, tea contains other beneficiary substances to our health such as fluoride, caffeine, minerals, trace elements e.g. manganese, chromium etc. which also added nutraceutical value of tea and its food additive which also add nutraceutical value of tea or its food additives . Looking to the therapeutic properties of these phytochemicals in tea, different food products have been prepared with tea or its extract which are rich in these active ingredients. These products include ready to drink beverages, instant tea, confections, cereal bars, pet foods, candy etc. So, there is a huge possibility of using bioactive constituents in tea as food additive which provide medical or health benefit in prevention and treatment of disease and thus tea can be potentially used for the development of nutraceuticals.
In this chapter, various bioactive compounds in tea (green, black, oolong and white teas) and their properties which can be employed as nutraceuticals in the form of food or part of food products has been discussed. Further, we have addressed the role of these tea nutraceuticals and their application in prevention and treatment of various diseases.
2. Phytochemicals in tea
There are a variety of chemicals in tea which are therapeutically important and they also vary in different types of commercially available teas viz. green, black and oolong tea. The chemical characteristics of tea are also varying with variation of plant species, geographic locations, climatic condition, horticultural practices as well as plucking parameters of the tea leaves [16, 17]. Polyphenols comprises of a large group of phytochemicals, the major polyphenolic constituents in tea which account for maximum 30% total dry weight of tea leaves are the flavanols, also known as catechins. Of these polyphenolic catechins, eight catechins are appeared in significant quantities which include (+)-catechin, (-)- epicatechin, (-)-gallocatechin, (-)-epigallocatechin, (-)-catechin gallate, (-)-epicatechin gallate, (-)-gallocatechin gallate, (-)-epigallocatechin gallate.
Tea beverage is chemically characterized mainly by polyphenolic constituents (mostly flavonoids) and their polymerized compounds. Four polyphenolic catechins which are in large quantities in green tea are (–)–epigallocatechin- 3-gallate (EGCG), (–)–epigallocatechin (EGC), (–)–epicatechin-3- gallate (ECG), and (–)–epicatechin (EC) (Table 1). These catechins are highest in green tea (30-40% in dry weight) and least in black tea (10% in dry weight) in which catechins undergo condensation and polymerization into other phenolic compounds theaflavins and thearubigins during enzymatic oxidation of catechins during fermentation process of black tea manufacturing [18, 19]. The oolong tea has substantial amount of catechins with other oligomeric polyphenols .
Apart from polyphenols, there are a number of phytochemicals which are significantly important in medicinal use. These phytochemicals are oxyaromatic acids (gallic, caffeic, quinine, chlorogenic and n-coumaric acids, flavonols (Quercetin, kaempferol, myricetin), pigments (carotenoids, chlorophyll), alkaloids (caffeine, theophylline, theobromine), amino acids, lipids, polysaccharides, vitamins, lignans and saponins which also attribute to the widely explored medicinal value of tea [21, 22, 23].
The major polyphenolic compounds in different types of tea which differ due to their manufacturing processes are highlighted in Table 2. The white tea is least processed of all the tea types and thus it intakes maximum polyphenols whereas the black tea is fully fermented through enzyme mediated oxidation of polyphenols into oligomeric and polymeric flavanols (theaflavins, thearubigins and other oligomers) with characteristics flavor and color. In black tea processing, tea leaves (standard plucking two and a bud) are crushed to undergo enzymatic oxidation (polyphenol oxidase) and subsequent condensation of tea catechins in to the formation of theaflavins (TFs) (oligomeric) and thearubigins (TRs) (polymeric) products. This fermentation process is being limited in case of oolong tea. In green tea this enzymatic oxidation is prevented by steaming (or pan-frying) of fresh tea leaves. Therefore, the green tea infusion contains major quantities of polyphenol catechins (30-40% dry weight of four major catechins viz. (–)-Epigallocatechin-3-gallate (EGCG), (–)-epigallocatechin (EGC), (–)-epicatechin-3-gallate (ECG), and (–)-epicatechin (EC)). Since all major polyphenols are oxidized in black tea, it contains less amount of catechins (3-10%) along with theaflavins (2-6%), thearubigins (>20%) and gallic acids in significant quantities. The oolong contains green tea catechins and less amount of black tea theaflavins and thearubigins due to its partial fermentation process.
|Type of tea||Processing technique||Physio-chemical changes||Major polyphenols|
|Green||Withering, steaming (Japan) or roasting (China), rolling and drying||Deactivation of enzymes to prevent fermentation (oxidation of polyphenol)||Catechins (Flavan-3-ols)|
|Black (red tea)||Withering, crushing, rolling, drying||Fermented (in presence of enzymes (polyphenol oxidase and peroxidase enzymes)||Catechins, theaflavins, thearubigins|
|Oolong||Withering, rolling, drying||Partially fermented (partial oxidation)||Catechins, theaflavins, procyanidins|
In water extract, all types of tea also contain caffeine in quantity of 2-5%.
3. Nutraceutical properties of tea phytochemicals
The therapeutic properties of tea extract are associated with the polyphenolic contents which have the highest antioxidant capacity amongst other bio-active compounds. The green tea extract has higher antioxidant capacity than that of black tea or oolong tea due the higher quantities of catechin polyphenols viz. EGCG, EGC, ECG, EC . The EGCG has the highest anti-oxidant activity followed by ECG and EC whereas EGC has the lowest activity . The EGCG can inhibit the production of hydrogen peroxide and superoxide radicals by tumor promoter-activated neutrophils in our body. Among the three major types of tea (green, black, oolong), green tea has the highest antioxidant activity and black tea has the lowest one. The antioxidant activity of green tea and its polyphenols have been studied in a number of
Recent researches have gained attention in therapeutic use of tea polyphenols of green and black tea in diseases associated to metabolic syndromes. Green tea catechins have been explored to antioxidant, anticarcinogenic, anti-inflammatory, probiotic, anti-inflammatory, thermogenic, and antimicrobial activities. These have been found to inhibit carcinogenesis of the skin, lung, esophagus, stomach, liver, small intestine, colon, bladder, prostate, and mammary gland in animal studies . Black and green tea can protect oxidative damage of red blood cells .
Looking in to the various health benefits of tea phytochemicals, tea extracts in liquid or powder form can be effectively used in food products such as sweets, biscuits, bread, cake, candies, ready-to-drink beverages as well as other polyphenol rich food supplements. The tea nutraceuticals are also available in various form of tablets, capsules and health drinks. In addition, tea also contains other nutritional substances such as dietary fiber and proteins after polyphenols extraction in tea for use in nutraceuticals which also give health benefits . Consumption of tea with lemon juice (ascorbic acid) has manifold benefits in making more accessible of tea antioxidants (catechins) to get absorbed as well as enhance the iron absorption in our body . Further, compounds in tea can inhibit iron absorption from foods; drinking green tea with lemon reduces that effect. The combination of honey with green tea has been used since very past as they are very effective in healing wounds. The health-promoting activities of tea also vary cultivar to cultivar and thus it is important to identify the cultivars having specific bioactivity so as to use the nutraceutical properties properly. The phytochemicals in tea and their nutraceutical properties are depicted in Figure 1.
4. Tea phytochemicals as Nutraceuticals in different diseases
Tea is beneficial in protection and prevention of numerous critical diseases such as different cancers, diabetes, neurodegenerative and cardiovascular diseases [29, 30, 31]. The polyphenols, the major phytochemicals present in tea are known for their antioxidant properties due to their abilities to scavenge free radical species such as hydroxyl radical (OH), hydrogen peroxide (H2O2) and superoxide anion (O2.−) and thus their consumption confer them as potential cancer chemopreventive agents as well as other free radical induced diseases. The polyphenol extract of tea has been effectively used in cancer prevention, protection cardiovascular diseases, reduce weight-loss, anti-allergic, prebiotics, osteoarthritis protection etc. [32, 33]. Green tea extract comprises of nearly 40% of polyphenols of which nearly 25% accounts for different catechins and their gallates of which EGCG is the highest in quantity (about 11%), the most studied polyphenols in therapeutic properties. Black tea polyphenols theaflavins, theaflavin gallates (which are dimers of two different catechins and their gallates) and thearubigins with 2-6% in quantity in addition to 3-10% of catechins gain attention in different diseases .
Herein, we wish to discuss the health benefits of tea in common and major diseases such as diabetes, cardiovascular diseases, obesity, cancers, as well as others minor diseases related to microbial and inflammatory types. The role of major phtochemicals of tea in different diseases is outlined in Table 3.
4.1 Tea phytochemicals in diabetics
Diabetes mellitus, a highly alarming disease worldwide is associated with high levels of blood glucose other than normal which is associated with insufficient insulin production or its ineffectiveness.
Polyphenols in green and black tea are very effective in reducing hyperglycemia and insulin resistance which can manage the blood glucose in diabetes [31, 35, 36].
Thus, tea polyphenols present potential nutraceuticals for various facets of type 2 diabetes mellitus. In type 2 diabetes, insulin tolerance is developed following major metabolic disorder which led to numerous health complications reducing the quality of life and increasing the mortality rate . It has become evident from various
It has been reported that polyphenols have the hypoglycemic effects which are associated in reducing intestinal absorption of dietary carbohydrates affecting glucose metabolism, improvement of β-cell function and insulin action, inducing insulin secretion, and anti-inflammatory and anti-oxidative activity .
4.2 Tea photochemicals in cardiovascular diseases
The cardiovascular diseases such as hypertension (high blood pressure), coronary heart diseases (heart attack), cerebrovascular disease (stroke), heart failure, peripheral vascular diseases, etc. have been increasing day by day. The major causes of these are foods habits, chemicals used in food and food products, chemicals used in cultivation as well as lack of awareness.
Green tea consumption has been found to be effective in reduction of cardiovascular related disorders through decreased serum cholesterol and triglyceride. It has been reported in a study carried out in Japan that regular green tea consumption (≥500 mL) reduced the mortality rate in women in cardio vascular diseases by 31% as compared to those who consume non-regularly .
The green consumption has the effect of reducing the risk of coronary heart diseases by lowering the hyperlipidemia and total body cholesterol in body . Moreover, EGCG the major catechin in green tea has been shown its usefulness in cardioprotective effects (inhibiting the formation of cardiac hypertrophy), antithrombotic and antiplatelet activities which are associated with cardiovascular diseases . The theaflavin and thearubigins in black tea functional drinks are valuable against lipid and glucose related abnormalities especially high cholesterol and LDL levels .
4.3 Tea phytochemicals in obesity
Obesity has been considered as a serious concern globally effecting large number of peoples. It is associated with accumulation of excess body fats which stimulate various disorders in body such as hypertension, osteoarthritis, hyperlipidemia, heart attack, etc.  It is identified as a major factor in many metabolic syndrome such as stroke, heart failure, hypertension, diabetics, arthritis, etc.. The oolong tea food products also were also found to be very effective in decrease of in weight of obese people . The green tea catechin, EGCG has been found to be beneficial on obesity . Catechins in tea helps in weight management and consumption of green tea catechins results significant reduction in body weight in human .
4.4 Tea phytochemicals in oral diseases
Tea phytochemicals are useful in treatment and prevention of various types oral diseases which include tooth decay and oral cancers [47, 48], dental plaque and dental caries . The tea extract and fluoride in tea can prevent the carcinogenic activity of oral bacteria such as
4.5 Tea phytochemicals in cancers
Tea nutraceutical acts as powerful antioxidants are very much promising in prevention of cancers. Experimental studies have demonstrated the use of tea bioactive compounds in prevention of cancers in different organs . The tea catechins has been found to act as anticarcinogenic and chemopreventive agents. Several animal model studies in mice showed the inhibiting of chemical induced 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) tumorigenesis, tumor cell proliferation, progression of adenoma to carcinoma, as well as lung carcinogenesis. . It has been conferred from several experimental evidences that tea can be effectively used in inhibition of carcinogenesis in different parts of our body such as skin, lung, stomach, esophagus, liver, small intestine, pancreas, colon, bladder, etc. . In these experimental studies it was found that polyphenols in green tea acted on cell apoptosis, cell proliferation, cell cycle in tumour growth and migration. All these activities resulted in reduced the risk of certain types cancers such as skin, colorectal, prostate, breast etc. through their activity in cell apoptosis, cell proliferation, cell cycle in tumour growth and cell migration. Among four major catechins in green tea, EGCG has been extensively studied and found to be most effective polyphenols . Theaflavins in black tea inhibit the DNA damage which is a major cause of induction of cancer  The anti-carcinogenic activity of tea polyphenols is associated with their capacity to bind with carcinogens and thereby enabled the metabolism. Cancer preventive activity of black tea is known from the report of induction of apoptosis in human leukemia cells by black tea and its polyphenols . Black tea is also effective in protecting immunocytes from tumor-induced apoptosis .
4.6 Other applications of tea phytochemicals
Consumption of tea has other several promising-health benefits such as it decrease the risk of hypertension , lowers the risk of osteoporosis, protects against the risk of hip fractures [59, 60], improves the metabolic activity of the bacteria in intestinal tract .
The bioactive compounds in oolong tea such as polyphenol, caffeine have the antistress and antioxidant activities which can prevent the diseases related to stress . The green tea catechin, EGCG is useful in inhibition of HIV infection and HIV-1 replication and multi-drug resistant
Some other beneficial effects of the constituents are antiaging, stress control, ant-bacterial, anti-viral, and useful in Parkinson’s disease, Alzheimer’s disease etc. Recently, Tencomnao et al.  discussed the results of some
|Phytochemicals of tea||Health benefits|
|Polyphenol extract of green tea||Cancer prevention, protection cardiovascular diseases, reduce weight-loss, anti-allergic, prebiotics, osteoarthritis protection etc.|
|Epigallocatechin-3-Gallate (EGCG), Epigallocatechin (EGC), epicatechingallate (ECG)||Anticancer, cardioprotection, Neuroprotection, Obesity management, osteoprotection, Anti-inflammation, Diabetes control and renal protection, Antimicrobial and skin care etc.|
|Theaflavins, Thearubigins, bisflavonols||Antioxidative, antithrombogenic, and anti-inflammatory, anti-carcinogenic, anti-mutagenic, neuroprotective etc.|
|Green tea supplements||Observed effects||
|Polyphenol extract||Increase in hydroxyproline content and catalase activity, Decrease in protein carbonyl content, Inhibit protein oxidation induced by UV radiations.||Mice|
|Aqueous extracts||Increase the level of collagen and elastin fibers.
Reduced expression of MMP-3 enzymes.
|Green tea extract||Reduce muscle atrophy and mediate insulin resistance, Reduce fat accumulation and lipid droplets.||Mice,
|Epigallocatechin gallate (EGCG)||Reduce the number of cells affected by sunburn, Maintain equilibrium during redox reaction.|
|Cholinergic acid||Delay age-related decline in body movements||
|Theanine||Stress resistance and lifespan extension.||C. elegans|
|Crude green tea extract||Reduction in total body iron.||Fruit flies|
|Green tea supplements||Observed effects||
|Polyphenol extract||Conjugate metabolites in plasma, blister fluid, and skin biopsy samples||Consume capsules of green tea polyphenols|
|Green tea extract||Reduction in the level of cells with sunburn; Lesser DNA damage when compared to vehicle control; Reduced the prevalence of Alzheimer’s disease and cognitive impairment; Free radical scavenging and anti-wrinkle effects||Topical application|
|EGCG, EC, and EGC||Decreased sunburn cells (Lesser activity when compared to the crude extracts).||Topical application|
|Polyphenol extract||Improvement in facial skin and in controlling erythema||Oral supplements of green tea polyphenols|
This chapter discusses the nutraceutical properties of bioactive compounds in tea in particular polyphenols and their role in health benefits. The catechins in tea viz. EGCG, EGC, ECG and EC have therapeutic properties such as antioxidant, anti-diabetic, anti-microbial, anti-inflammatory as well as anti-cancer properties. The black tea polyphenols the flavins and thearubigins also show many profound therapeutic values. In addition, oolong tea and white tea are also pharmacologically important. Therefore, consumption of tea in its various form viz. green tea, black tea, oolong tea and other food products with tea as active ingredient have been increased now a days. There are many more food products with tea and or its extracted components as additive have been developed which includes instant tea beverage, confections, cereal bars, pet foods etc. Considering the growing interest in nutraceuticals due to their several health values, the aim of the present chapter is to outline the nutraceutical potential of phytochemicals of tea with their known therapeutic properties. Due to their ready bioavailability, the possibilities of using nutraceuticals from tea as natural healers in treatment and prevention of diseases have been explored.
Author specially thanks to the Tocklai Tea Research Institute, India for giving support during the tenure of working on tea research as scientist.
Eskin M, Tamir S. Dictionary of Nutraceuticals and Functional Foods. Boca Raton, USA: CRC Press. 2019.ISBN:9780367391508
Jemimah G, Keerthana M, Reddy TS, Bonagiri Y, Gupta AVSSS, Sharma JVC. Nutraceuticals for prevention of various disease conditions. Indo Am. J. P. Sci, 2019; 06(11). DOI:10.5281/zenodo.3533809
Brower V. Nutraceuticals: poised for a healthy slice of the healthcare market? Nat Biotechnol, 1998; 16(8):728-731. DOI:10.1038/nbt0898-728
Kalra EK. Nutraceutical – definition and introduction. AAPS Pharmsci, 2003; 5:12081212. DOI:10.1208/ps050325
Stagg GV, Millin DJ. The nutritional and therapeutic value of tea: A Review. Journal of Science of Food and Agriculture.1975; 26:1439-1459. DOI:10.1002/jsfa.2740261002
Xing L, Zhang H, Qi R, Tsao R, Mine Y. Recent Advances in the Understanding of the Health Benefits and Molecular Mechanisms Associated with Green Tea Polyphenols. Journal of Agricultural and Food Chemistry. 2019; 67(4):1029-1043. doi:10.1021/acs.jafc.8b06146
Harbowy ME, Balestine DA, Davies PA, Cai Y. Tea Chemistry. Critical Reviews in Plant Sciences.1997; 16(5):415-480. DOI: 10.1080/07352689709701956
Senanayake SN. Green tea extract: Chemistry, antioxidant properties and food applications−A review. Journal of Functional Foods.2013, 5(4):1529−1541. DOI: 10.1016/j.jff.2013.08.011
Wang Y, Ho CT. Polyphenolic chemistry of tea and coffee: a century of progress. Journal of Agricultural Food Chemistry. 2009; 57(18):8109-8114. DOI:10.1021/jf804025c
Lee KW, Lee HJ, Lee CY. Antioxidant activity of black tea vs. green tea. The Journal of Nutrition. 2002; 132(4):785-786. DOI:10.1093/jn/132.4.785
Higdon JV, Frei B. Tea catechins and polyphenols: health effects, metabolism, and antioxidant functions. Critical Reviews in Food Science and Nutrition.2003; 43(1), 89–143. DOI:10.1080/10408690390826464
Xing L, Zhang H, Qi R, Tsao R, Mine Y. Recent Advances in the Understanding of the Health Benefits and Molecular Mechanisms Associated with Green Tea Polyphenols. Journal of Agricultural and Food Chemistry. 2019; 67(4): 1029-1043. DOI:10.1021/acs.jafc.8b06146
Dufresne CJ, Farnworth ER. A review of latest research findings on the health promotion properties of tea. Journal of Nutritional Biochemistry. 2001; 12(7):404-421. DOI:10.1016/s0955-2863(01)00155-3
Sajilata MG, Bajaj PR, Singhal RS. Tea Polyphenols as Nutraceuticals. Comprehensive Reviews in Food Science and Food Safety.2008: 7:229-254. DOI: 10.1111/j.1541-4337.2008.00043.x
Ahmad M, Baba WN, Shah U, Gani A, Gani A, et al. Nutraceutical Properties of the Green Tea Polyphenols. Journal of Food Processing & Technology.2014; 5: 390. doi:10.4172/2157-7110.1000390
Robertson A. The chemistry and biochemistry of black tea production—the non-volatiles. In: Willson KC, Clifford MN, editors. Tea: Cultivation to Consumption. London, UK: Chapman & Hall. 1992:pp. 555–601
Sharma V, Gulati A, Ravindranath SD. Extractability of tea catechins as a function of manufacture procedure and temperature of infusion. Food Chemistry. 2005; 93(1): 141-148. DOI:10.1016/j.foodchem.2004.10.016
Yanishlieva-Maslarowa, NV, Heinonen, IM. Sources of natural antioxidants: vegetables, fruits, herbs, spices and teas. In: Antioxidants in food - practical applications. Eds. Pokorny J, Yanishlieva N, Gordon M. CRC Press, Woodhead Publishing, Cambridge, England. 2001:210- 249.
Balentine DA, Wiseman SA, Bouwens LCM. The chemistry of tea flavonoids. Critical Reviews in Food Science and Nutrition. 1997; 3:693-704. DOI: 10.1080/10408399709527797
Wang K, Liu F, Liu Z, Huang J, Xu Z, Li Y, Chen J, Gong Y &Yang X. Analysis of chemical components in oolong tea in relation to perceived quality. International Journal of Food Science and Technology 2010; 45: 913–920. DOI:10.1111/j.1365-2621.2010.02224.x
Lakenbrink C, Lapczynski S, Maiwald B, Engelhardt UH. Flavonoids and otherpolyphenols in consumer brews of tea and other caffeinated beverages. Journal of Agricultural and Food Chemistry. 2000, 48, 2848–2852. DOI: 10.1021/jf9908042
Lin JK, Lin CL, Liang, YC, Lin-Shiau SY, Juan IM. Survey of catechins, gallicacid, and methylxanthines in green, oolong, pu’erh, and black teas. Journal of Agricultural and Food Chemistry. 1998; 46, 3635-3642. DOI: 10.1021/jf980223x
Weisburger JH. Tea antioxidants and health. Handbook of antioxidants. New York. 1996.
Cabrera C, Giménez R, López MC. Determination of tea components with antioxidant activity. Journal of Agricultural and Food Chemistry. 2003 ;51: 4427-4435. DOI:10.1021/jf0300801
Namal Senanayake SPJ. Green tea extract: Chemistry, antioxidant properties, and food applications- A Review. Journal of Functional Foods.2013; 5(4):1529-1541. DOI:10.1016/j.jff.2013.08.011
Yang CS, Maliakal P, Meng X. Inhibition of carcinogenesis by tea. Annual Review of Pharmacology and Toxicology.2002; 42:25-54. DOI:10.1146/annurev.pharmtox.42.082101.154309
Grinberg LN, Newmark H, Kitrossky N, Rahamim E, Chevion M, Rachmilewitz EA. Protective effects of tea polyphenols against oxidative damage to red blood cells. Biochemical Pharmacology.1997; 54(9):973-978. DOI:10.1016/s0006-2952(97)00155-x
Lu TM, Lee CC, Maud JL, Lin SD. Quality and antioxidant property of green tea sponge cake. Food Chemistry.2010; 119: 1090-1095. DOI: 10.1016/j.foodchem.2009.08.015.
Butt M, Ahmad R, Sultan M, Nasir QM, Naz A. Green tea and anticancer perspectives: Updates from last decade. Critical Reviews in Food Science and Nutrition.2015; 55:6, 792-805. DOI: 10.1080/10408398.2012.680205
Mandel S, Yodim M. (2004). Catechin polyphenols: Neurodegeneration and neuroprotection in neurodegenerative diseases. Free Radical Biology and Medicine.2004; 37: 304-307. DOI: 10.1016/j.freeradbiomed.2004.04.012
Yang T, Koo M. Inhibitory effect of Chinese green tea on endothelial cell-induced LDL oxidation. Atherosclerosis.2000; 148: 67-73. DOI: 10.1016/S0021-9150(99)00239-7
Imran A, Butt MS, Arshad MS, et al. Exploring the potential of black tea based flavonoids against hyperlipidemia related disorders. Lipids in Health and Disease. 2018; 17(1):57. DOI:10.1186/s12944-018-0688-6
Alipoor B, Rad AH. A review on the therapeutical effects of tea. Asian J Clin Nutri. 2012; 4(1):1–15.DOI: 10.3923/ajcn.2012.1.15
Butt MS, Imran A, Sharif MK, et al. Black tea polyphenols: a mechanistic treatise. Critical Reviews in Food Science and Nutrition. 2014; 54(8):1002-1011. DOI:10.1080/10408398.2011.623198
Tachebele B, Abebe M, Birhan W, Addis Z. Health Benefits of Green Tea with Emphasis on Diabetes Mellitus. American Journal of Food and Nutrition. 2014; 2(5):85-91. DOI: 10.12691/ajfn-2-5-2.
Vinson JA, Zhang J. 2005. Black and green teas equally inhibit diabetic cataracts in a streptozotocin-induced rat model of diabetes. Journal of Agricultural and Food Chemistry. 2005; 53(9):3710–3713.DOI:10.1021/jf0480521
Santaguida PL, Balion C, Hunt D, et al. Diagnosis, prognosis, and treatment of impaired glucose tolerance and impaired fasting glucose. Evidence Report/Technology Assessment (Summary).2005; 128:1-11.PMCID:PMC4780988
Thielecke F, Boschmann M. The potential role of green tea catechins in the prevention of the metabolic syndrome - a review. Phytochemistry. 2009; 70(1):11-24. DOI:10.1016/j.phytochem.2008.11.011
Bahadoran Z, Mirmiran P, Azizi F. Dietary polyphenols as potential nutraceuticals in management of diabetes: a review. Journal of Diabetes and Metabolic Disorders. 2013; 12(1):43. DOI:10.1186/2251-6581-12-43
Kuriyama S. The relation between green tea consumption and cardiovascular disease as evidenced by epidemiological studies. The Journal of Nutrition.2008; 138(8):1548S-1553S. DOI:10.1093/jn/138.8.1548S
Pang J, Zhang Z, Zheng T, Yang YJ, Li N, Bai M, Peng Y, Zhang J, Li Q, Zhang B. Association of green tea consumption with risk of coronary heart disease in Chinese population. International Journal of Cardiology. 2015; 179:275-278. DOI: 10.1016/j.ijcard.2014.11.093. Epub 2014 Nov 11. PMID: 25464464; PMCID: PMC4843789.
Zheng J, Lee HC, Bin Sattar MM, Huang Y, Bian JS. Cardioprotective effects of epigallocatechin-3-gallate against doxorubicin-induced cardiomyocyte injury. European Journal of Pharmacology. 2011; 652(1-3):82-88. DOI:10.1016/j.ejphar.2010.10.082
Rossner S. Obesity: the disease of the twenty-first century. International journal of obesity and related metabolic disorders. 2002;26(Suppl. 4), S2–S4. DOI: 10.1038/sj.ijo.0802209]10.1038/sj.ijo.0802209].
Sae-tan S., Grove K. A., Lambert J. D. (2011). Weight control and prevention of metabolic syndrome by green tea. Pharmacological Research. 2011 ; 64:146–154. DOI:10.1016/j.phrs.2010.12.013
Suzuki T, Pervin M, Goto S, Isemura M, Nakamura Y. Beneficial Effects of Tea and the Green Tea Catechin Epigallocatechin-3-gallate on Obesity.Molecules. 2016;21(10):1305.DOI:10.3390/molecules21101305
Yang CS, Zhang J, Zhang L, Huang J, Wang Y. Mechanisms of body weight reduction and metabolic syndrome alleviation by tea. Molecular Nutrition and Food Research. 2016;60(1):160-174. DOI:10.1002/mnfr.201500428
Lee MJ, Lambert JD, Prabhu S, Meng X, Lu H, Maliakal P, Ho CT, Yang CS. 2004. Delivery of tea polyphenols to the oral cavity by green tea leaves and black tea extract. Cancer Epidemiology, Biomarkers & Prevention. 2004; 13:132–137: DOI: 10.1158/1055-9965.epi-03-0040
Hamilton-Miller JMT. Anti-cariogenic properties of tea (Camellia sinensis). Journal of Medical Microbiology.2001; 50(4):299-302. DOI:10.1099/0022-1317-50-4-299
Rasheed A, Haider M. Antibacterial activity of Camellia sinensis extracts against dental caries. Archives of Pharmacal Research. 1998; 21(3):348-352. DOI:10.1007/BF02975300
McKay DL, Blumberg JB. The role of tea in human health: an update. Journal of the American College of Nutrition. 2002; 21(1):1-13. DOI: 10.1080/07315724.2002.10719187.
Yang CS, Wang X, Lu G, Picinich SC. (2009) Cancer prevention by tea: animal studies, molecular mechanisms and human relevance. Nature Reviews Cancer. 2009; 9 ( 6 ): 429-439. DOI: 10.1038/nrc2641
Lambert JD, Hong J, Yang GY, Liao J, Yang CS. Inhibition of carcinogenesis by polyphenols: evidence from laboratory investigations. American Journal of Clinical Nutrition. 2005; 81(1 Suppl):284S-291S. DOI:10.1093/ajcn/81.1.284S
Das L, Bhaumik E, Raychaudhuri U, Chakraborty R. Role of nutraceuticals in human health. Journal of Food Science and Technology. 2012; 49(2):173-183. DOI:10.1007/s13197-011-0269-4
Du GJ, Zhang Z, Wen XD, et al. Epigallocatechin Gallate (EGCG) is the most effective cancer chemopreventive polyphenol in green tea. Nutrients. 2012; 4(11):1679-1691. DOI:10.3390/nu4111679
Feng Q, Torii Y, Uchida K, Nakamura Y, Hara Y, Osawa T. Black tea polyphenols, theaflavins, prevent cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P450 1A1 in cell cultures. Journal of Agricultural and Food Chemistry. 2002; 50(1):213-220. DOI:10.1021/jf010875c
Kundu T, Dey S, Roy M, Siddiqi M, Bhattacharya RK. Induction of apoptosis in human leukemia cells by black tea and its polyphenol theaflavin. Cancer Letters. 2005; 230(1):111-121. DOI:10.1016/j.canlet.2004.12.035
Bhattacharyya A, Mandal D, Lahiry L, Sa G, Das T. Black tea protects immunocytes from tumor-induced apoptosis by changing Bcl-2/Bax ratio. Cancer Letters. 2004; 209(2):147-154. DOI: 10.1016/j.canlet.2003.12.025.
Hodgson JM, Puddey IB, Burke V, Beilin LJ, Jordan N. Effects on blood pressure of drinking green and black tea. Journal of Hypertension. 1999; 17(4): 457–463. DOI:10.1097/00004872-199917040-00002
Gardner EJ, Ruxton CHS, Leeds AR. Black tea – helpful or harmful? A review of the evidence. European Journal of Clinical Nutrition.2007; 61(1) 3-18. DOI:10.1038/sj.ejcn.1602489
Kanis J, Johnell O, Gullberg B, et al. Risk factors for hip fracture in men from southern Europe: the MEDOS study. Mediterranean Osteoporosis Study. Osteoporosis International. 1999; 9(1):45-54. DOI:10.1007/s001980050115
Weisburger JH. 2003. Prevention of coronary heart disease and cancer by tea, a review. Environmental Health and Preventive Medicine. 2003; 7:283–288. DOI: 10.1007/BF02908887
Kurihara H, Chen L, Zhu B, He Z, Shibata H, Kiso Y, Tanaka T, Yao X. Anti-stress effect of oolong tea in women loaded with vigil. Journal of Health Science.2003; 49(6):436–43.DOI:
Williamson MP, McCormick TG, Nance CL, Shearer WT. Epigallocatechin gallate, the main polyphenol in green tea, binds to the T-cell receptor, CD4: potential for HIV-1 therapy. Journal of Allergy and Clinical Immunology. 2006; 118 ( 6 ):1369–1374.DOI:10.1016/j.jaci.2006.08.016
Camargo LEA, Pedroso LS, Vendrame SC, Mainardes RM, & Khalil NM. Antioxidant and antifungal activities of Camellia sinensis (L.) Kuntze leaves obtained by different forms of production. Brazilian Journal of Biology, 2016;76(2), 428–434. DOI:10.1590/1519-6984. 18814.
Fortunato LR, Alves CDF, Teixeira MM, Rogerio AP, Quercetin: a flavonoid with the potential to treat asthma, Brazilian Journal of Pharmaceutical Science.2012; 48(4):589–599.
Toyoda M, Tanaka K, Hoshino K, Akiyama H, Tanimura A, Saito Y, Profiles of potentially antiallergic flavonoids in 27 kinds of health tea and green infusions, Journal of Agricultural and Food Chemistry 199745 (7), 2561-2564. DOI:10.1021/jf970024y
Prasanth MI, Sivamaruthi BS, Chaiyasut C,Tencomnao T, A Review of the Role of Green Tea ( Camellia sinensis) in Antiphotoaging, Stress Resistance, Neuroprotection, and Autophagy, Nutrients 201911(2), 474. DOI:10.3390/nu11020474