Open access

Introductory Chapter: Recent Advances in Rice Biotechnology for Abiotic Stress Tolerance

Written By

Munazza Ijaz, Roshina Shahzadi, Akmaral U. Issayeva, Shazia Anwer Bukhari and Mahmood-ur-Rahman

Submitted: 21 July 2020 Published: 03 March 2021

DOI: 10.5772/intechopen.94036

From the Edited Volume

Recent Advances in Rice Research

Edited by Mahmood-ur- Rahman Ansari

Chapter metrics overview

622 Chapter Downloads

View Full Metrics

1. Introduction

The world population is rising gradually, and it would be approximately 9.1 billion in 2050 but the production of agriculture is not escalating with this speed. The global production of agriculture should be increased up to 100-110 percent till 2050 to feed the whole population. Moreover, agricultural production and crop growth are negatively affected by abiotic stresses. So, it is a major hurdle in the way of increasing food production world-wide. The drought, heat, cold, salinity, ultra violet radiations and heavy metal toxicity are the major abiotic stresses that affect the crop yield. The significant source of calories for human beings is cereals. The maize, wheat and rice produce 10%, 17% and 23% respectively. Oryza sativa (rice) serve as a staple food and a famous cost-effective cereal. Acceptance of green revolution cultivars lead towards an essential revolution in the production of rice [1]. First green revolution came into being by producing high-yielding semi dwarf rice and wheat cultivars via plant breeding and providing high doses of nitrogen fertilizers. It helped in preventing the famine in semi-arid regions of Southern Asia [2].

The first transgenic rice was developed almost two decades before that initiated the high-throughput transformation protocols for the development of high yielding cultivars and it laid the foundation of transgenic rice biotechnology [3]. After the genome sequencing of rice, the development of transgenic rice varieties for better stress tolerance, high yield and better nutritional qualities became relatively easy and gained more importance. Similarly, the donor genes from the other species (like bacteria, fungi, viruses, animals and insects) can be inserted in the rice for obtaining the improved cultivars for making agriculture more sustainable. Improvement of some other significant traits (like photosynthetic rate, aroma and nutrition of rice grains) with the good stress tolerance has gained attention in past few years [2]. Some examples of these traits will be discussed in the next sections comprehensively.

The transgenic or genetically improved crops are an integral part of the agriculture industry in the modern world. The transgenic cereal crops have been commercialized and cultivated by more than twenty-eight countries. In 1996, only 1.7 million hectares of land have been cultivated with the transgenic crop that was increased up to 170 million hectares till 2012 and still it is increasing. Continuous efforts are made to bring the rice yield maximum for developing the large market of it globally [2]. Therefore, it is necessary to develop more cultivars of rice by using either old plant breeding techniques or new targeted genome editing techniques like zinc finger proteins (ZFNs), transcription activator-like nucleases (TALENs) and clustered regularly interspaced short palindromic repeats associated with Cas9 protein (CRISPR-Cas9) to develop cultivar of rice for improved agronomical traits.


2. Rice biotechnology for abiotic stress tolerance

Rice is an economically important crop and serious efforts are needed to increase its production. The production of rice can be increased in various ways like use of the available agricultural land at its maximum, utilization of marginal lands for growing the rice crop and using the lands effected by various abiotic stresses (e.g. drought stress, heat stress, cold stress, salt stress, UV radiation stress and metal toxicity) by growing the transgenic rice cultivars that are developed with the help of biotechnology. This is necessary because the agricultural land is decreasing and demand for food is increasing with the increasing world population and it is becoming very difficult to meet the food demands of this exponentially growing population with the same efficiency of the present day cultivars of rice [4].

The production of rice is greatly influenced by various abiotic stresses and it can be compensated by the various genes and regulatory networks present in different stress tolerant cultivars of rice and these genes and networks can be taken from the other plant species that can be used for developing new cultivars of rice (Figure 1). Abiotic stresses can induce the expression of many genes. The complex transcriptional networks are responsible for regulating the induction of stress tolerant genes. The important genes that are involved in transcriptional networks are studied by molecular techniques and these genes are useful in developing abiotic stress tolerant transgenic rice [5]. So, various biotechnology methods are used to develop rice cultivars that can combat with abiotic stresses (Table 1).

Figure 1.

Abiotic stresses (e.g. drought, cold, heat, salinity, UV radiations, heavy metals) follow a same mechanism to survive and retain their growth and development. It has four main steps that are (a) avoidance: avoiding the contact with stresses, (b) escape: changing the life-cycle, (c) recovery: vegetative growth potency and (d) tolerance: nullifying the impacts of stresses.

S. No.Transgene(s)Improved Trait(s)References
1.OsHsp101, AtHsp101Heat stress tolerance enhanced[6]
2.OsMAPK44Drought and salinity stress tolerance enhanced[7]
3.OsPIP1;3Cold stress tolerance ability enhanced[8]
4.Choline mono-oxygenaseHeat and salinity stress tolerance enhanced[9]
5.AVP1, SsNHX1Improved ROS and salinity stress tolerance[10]
6.OsSBPaseImproved photosynthetic efficiency and heat tolerance ability[11]
7.HvCBF4Salinity, drought and cold tolerance capacity enhanced[12]
8.SUB1AEnhanced submergence tolerance[13]
9.OsDREB1FSalinity, drought and cold tolerance capacity enhanced[14]
10.STAR1, STAR2Enhanced tolerance to Aluminum toxicity[15]
11.ZFP245Improved ROS, cold and drought stress tolerance[16]
12.P5CSF129ASalinity stress tolerance enhanced[17]
13.Isoflavone reductaseSalinity stress tolerance enhanced[18]
14.OsHMA3Enhanced drought and submergence stress tolerance[19]
15.OsMAPK2Tolerance to phosphate deficiency[20]
16.OsNAC5Salinity, drought and cold tolerance capacity enhanced[21]
17.OsLEA3-2Salinity and drought tolerance capacity enhanced[22]
18.OsMYB55Heat stress tolerance enhanced[23]
19.PCK, PPDKImproved ROS stress tolerance[24]
20.OsETOL1Enhanced submergence stress tolerance[25]
21.OsMYB48-1Salinity and drought tolerance capacity enhanced[26]
22.VrDREB2AIncreased tolerance to salinity and drought stress[27]
23.TaMYB3R1Enhanced drought and salt stress tolerance[28]
24.CaPUB1Enhanced cold stress tolerance[29]
25.OsLEA4Enhanced drought, salt and heavy metal stress tolerance[30]
26.OsNAC2Enhanced drought and salt stress tolerance[31]
27.OsGSImproved ROS and drought stress tolerance[32]
28.TsPIP1;1Enhanced salinity stress tolerance[33]
29.RhMYB96Enhanced salt tolerance[34]

Table 1.

Some transgenes inserted for improving the abiotic stresses.

2.1 Drought stress

Drought is affecting agricultural land worldwide from few past years. Many molecular, physiological and metabolic changes occur in plants due to drought stress that damages their growth and development [35]. During drought stress, plants respond variously and express change in their physiology and morphology. Rice drought resistance is achieved by four procedures that are (a) avoidance: avoiding the contact with stress, (b) escape: changing life-cycle, (c) recovery: vegetative growth potency and (d) tolerance: nullifying the impacts of stress. These procedures are fulfilled by variable mechanisms like reduced leaf area, leaf rolling, senescence of older leaves, increased root proliferation, dense root system, scavenging reactive oxygen species (ROS), early flowering, osmotic adjustment, stomatal closure that minimize the water loss, changes in elasticity of cell wall and maximum uptake of deep water allow plants to survive during extended periods of drought and even allow them to reproduce in limited water supply by maintaining physiological activities [36]. Osmotic potential is kept lower inside the plant cells than outside the cells by an important mechanism known as osmotic adjustment. So, it allows the plants to retain its turgidity and prevents the water loss. Drought stress tolerance in plants can be achieved by the accumulation of inorganic and organic substances like proline, potassium ions, glucose and sucrose.

This mechanism participates in osmotic adjustment and maintains the turgor pressure. Plants exposed to drought stress produce high level of ROS that are extremely toxic and damages the DNA, carbohydrates, proteins and lipid. In drought tolerant plants, non-enzymatic antioxidants and antioxidant enzymes are produced that protect plants from the deleterious effects of ROS. Currently, the production system of rice depends upon the excessive supply of water and therefore, it is more susceptible to drought stress. The drought stress is the most significant restrictive factor in the production of rice and it is becoming a very severe problem. The varieties of rice favored by farmers in the tropical and sub-tropical regions are vulnerable to drought stress [35]. Rice is a significant economic crop of Asia that is mainly cultivated in lowland regions, where agriculture depends on the seasonal rainfall. Thus, rice crop in these areas is very susceptible to drought stress but this problem can be rectified by introducing some drought tolerant genes in the economically important cultivars of rice. For example, jasmine rice or ‘Khao Dawk Mali 105’ (KDML105) is a world-famous cultivar of rice exported from Thailand, had suffered from drought stress because of limited irrigation and unpredictable rainfalls. Drought tolerance can be achieved after keeping genetic background of KDML105 conserved by chromosome-segment substitution lines (CSSL) which have drought-tolerant quantitative-trait loci (DT-QTL) obtained from the back crossing between drought tolerant donor IR58586-F2-CA-143 (DH212) and KDML105. For more understanding, the drought tolerance related physiological responses consider another CSSL named as CSSL1-16. This line has properties like high proline, high water status, good membrane stability and great osmotic adjustment. Furthermore, it can recommence growth after recovery from stress. So, it can be used as a potential candidate for developing further drought tolerant cultivars while keeping their own genetic background preserved [37].

Another example of producing drought tolerant rice plants is introduction of pea DNA Helicase-47 (PDH47) from the plant Pisum sativum by using a constitutive promoter of cauliflower mosaic virus 35S- CaMV had been introduced in the ASD16 a cultivar of Indica rice via Agrobacterium tumefaciens. The transcripts of PDH47 are upregulated during drought stress that is correlated with increased water status, hydrogen peroxide accumulation and proline accumulation. It also regulates many stress responsive genes present endogenously during drought stress in transgenic rice [38]. There are many more genes that are expressed during the drought stress in different plants and are also potential candidates for producing transgenic rice that are drought tolerant.

2.2 Heat stress

Heat stress is a major limiting factor in the production of crops across the world because of global warming. A negative correlation is present between the increased temperature and crop yield especially in case of rice, wheat, barley and maize [39]. The heat stress can damage rice plants severely by inhibiting the metabolic activities, seed setting, plant growth and pollen fertility; therefore production of rice is reduced [40]. Excessive heat can also decrease the photosynthetic ability of plant, reduce water use efficiency, seed weight and grain mass and shorten the leaf area. Heat stress can damage both at vegetative and reproductive stage from sprouting to maturity. But, flowering and booting are the two more critical stages that can cause complete sterility in rice cultivars [41]. Heat tolerance belongs to plants that can lessen the effects of stress and give enough economic yields even at high temperature. Like other plant species, rice also has variations in germplasm to combat with heat stress. Tolerance is achieved by adjusting different molecular, morphological and physiological traits in rice cultivars. High temperature enhances the expression of stress tolerating genes and increases the metabolite production which is beneficial for achieving stress tolerance in plants [42]. During heat stress plants adopt multiple mechanisms like avoidance, survival and escape. These mechanisms impose avoidance for short term and develop resistance for long term survival. At the cellular level, effects of stress can be neutralized by certain factors and mechanisms; such as transcriptional control, antioxidant defense, osmolytes, late embryogenesis abundant (LEA) proteins and the factors that participate in signaling cascades. In hot environments, yield is reduced because of early maturity comes under the domain of avoidance strategies while suffering from heat stress [41].

Several morphological markers like long anthers, high pollen fertility, long basal pores and large basal dehiscence can be used for determining heat tolerant rice. As well as grain yield, number of spikelet, weight of thousand grains and seed setting percentage can be used for screening rice germplasm against high temperature stress; a great reduction is found in all these factors during heat stress. So, these parameters can be used to screen heat tolerant rice. Opening of the rice spikelet in early morning can be another beneficial criteria for the selection of heat tolerant rice cultivars [41]. A thermo-tolerant wild cultivar known as Oryza meridionalis can maintain high photosynthesis rate at high temperature. This high rate of photosynthesis is due to good stability and activity of Rubisco and it can be used as a significant physiological marker to determine the heat tolerance in rice [43]. The chlorophyll content and electrolyte leakage from leaves and roots can be increased during heat stress; it can also be used as a marker to analyze the heat tolerance. The fluid content of the membrane, lipid molecule present in membranes, cooling effect of transpiration and leaf position are some other beneficial physiological marker to determine the heat tolerance in rice.

Plant breeding efficacy has been increased by using marker assisted selection (MAS). Numerous molecular markers are used in MAS, but, single nucleotide polymorphism (SNPs) and simple sequence repeats (SSRs) are extensively used. This procedure is used to gather the information about the genes involved in providing resistance against biotic and abiotic stresses [44]. Still, there are very limited numbers of rice cultivars that are engineered to tolerate heat stress. Because the SNPs are present more than the SSRs in the rice genome, so SNPs are more prone to use for developing heat tolerance. A few SNPs related with heat tolerance have been known. Due to the very complex nature of this trait, each marker can contribute very less towards the variance. Therefore, several markers that are linked with various quantitative trait loci (QTLs) are used to improve a cultivar for heat tolerance [45]. Many genes are responsible for heat tolerance at various stages; for example, ZFP is a gene that is related with heat tolerance at seedling stage [46] and OsWRKY11 is another gene that is responsible for heat tolerance in rice [47]. The heat tolerant genes are needed to be identified in rice cultivars and the genes from the other plant species can also be transferred to the rice cultivars to improve the heat tolerance. Other approaches like use of mutagens and a recent technology like CRISPR-Cas9 should be applied for further development of heat tolerant rice cultivars.

2.3 Cold stress

The cold stress is a significant environmental factor that affects the development and growth of rice crop. At the stage of seedling development, sudden decrease in temperature can influence the development of chlorophyll [48]. The damage of rice seedling due to cold stress ultimately decreases the grain yield. So, the cold stress is a major limitation which can be overcome by using cold tolerant rice varieties [49]. Because rice crop is evolved in tropical region, so it has limited adaptability to chilling stress. Improving rice varieties to make them cold tolerant, enable the cultivation of rice in northerly latitudes. Different signal transduction pathways and genetic networks are involved in controlling chilling tolerance [50]. In japonica rice, chilling tolerance is achieved by interactions between rice G-protein α-subunit 1 (RGA1) and chilling tolerance divergence 1 (COLD1) followed by the calcium signaling initiated in the response of downstream network of stress response belongs to C repeat binding factor (CBF) that is a transcription factor [51]. Still, there is limited information available about the stress response and adaptation. The plant show response to abnormal surrounding temperature by changing their gene expression and adapt the suitable architecture due to the developmental plasticity. In SAM (shoot apical meristem) intrinsic signals can be disrupted by cold stress and stress tolerance is enhanced by setting the dormancy cycling at the SAM [52]. For the survival mechanism against cold stress, protection of niche forms of root stem cell is a sacrifice [53]. The differentiated cells are properly organized, and meristematic activity is maintained by this rehabilitated development in response to the cold temperature. During cold stress, many specific genes like OsMYB3R-2 are activated by the various transcription factors to maintain the mitotic cell and their cold tolerance. The survival and growth are enhanced by keeping maintained the cellular activity and cell behavior during and after the cold stress.

The formation of axillary bud following by its outgrowth in axils of primordia of leaf controls the shoot branching. The axillary bud initiation is controlled by MOC1 (Monoclum 1) and LAX 1 (Lax Panicle 1). The signaling pathways involved in the axillary bud outgrowth formation and biosynthesis of strigolactone are important for tillering in rice crop and are controlled by the dwarf genes, that are dwarf 53 (D53), dwarf 27 (D27), dwarf 17 (D17), dwarf 14 (D14), dwarf 10 (D10) and dwarf 3 (D3) [54]. In rice the outgrowth of axillary buds is repressed by the OsTB1 that acts in the downstream region of the dwarf genes. The interaction between the OsMADS57 and OsTB1 is responsible for reducing the inhibitory effect on the D14, a gene responsible for the organogenesis of rice tillers by producing a receptor for the hormone strigolactone [55], and it enables D14 to regulate the initiation of axillary bud [56]. The plant endogenous conditions and environmental situations control the development of axillary buds that are basically indeterminate structures [57]. To adapt the cold environment a network has been identified having core gene OsMADS57. The rice tiller growth is maintained by the overexpression of OsMADS57 during cold stress. OsMADS57 directly binds with the promoter of the OsWRKY94 gene and enhances its expression in response to chilling stress while retarding its expression during normal temperature. Moreover, during normal temperature and cold stress, OsTB1 directly target and suppress the OsWRKY94 gene and under the cold stress OsMADS57 is responsible for promoting the transcription of D14 and suppress the tillering while during normal conditions expression of D14 was repressed to enhance the tillering. It shows that OsTB1 and OsMADS57 equally contribute to develop the cold tolerance in rice by targeting OsWRKY94 [52]. So, they can be used as potential candidates for developing cold tolerant rice cultivars.

Cold tolerance can also be achieved by the biogenesis of chloroplast in rice crop. In plant development and growth, chloroplast plays an important role and its development is affected by the chilling temperature. The genes and regulators that are involved in the biogenesis of chloroplast are identified and characterized. The mutant of WSL5 (white strip leaf 5) in rice has been characterized. The white stripped leaves are developed by this mutant during early leaf development stage and show albino phenotype during chilling stress. A unique chloroplast-targeted-pentatricopeptide repeat protein is encoded by the WSL5; the molecular and genetic analysis has revealed it. The RNA sequence analysis revealed that in the mutant, expression of the nuclear-encoded-photosynthetic genes was significantly repressed and the genes responsible for the chloroplast formation were also changed significantly. WSL5 gene is required for the development of chloroplast during cold stress [58]. A transcription factor OsMYB3R-2 from rice having a DNA binding domain is involved in enhancing the cold tolerance [59]. Another transcription factor MYBS3 of rice having a DNA binding repeat also play a significant role in adapting the cold environment [60].

The progress in developing the genetically modified plants by introducing and overexpressing the novel genes appears to be a very good practically possible option to speed up the process of breeding in plants. Instinctively, the faster way of inserting the genes with beneficial traits is genetic engineering instead of molecular or conventional breeding; and it would be the only possible option if the gene of interest belongs to some other species of plants, distant relatives, or from any non-plant source. The utilization of gene knockout strategies and genomic approaches are developing to enhance the efforts to measure thoroughly and make it easy to completely understand the complicated quantitative traits like tolerance to different temperature fluctuations and extremes. Many relevant genes have been identified by using molecular and genetic techniques to understand the plant response for the cold stress and these genes can be used further for developing cold tolerant varieties [61].

2.4 Salt stress

Rice crop is susceptible to salt stress and 1/3 of total world agricultural land is affected with it. The salinity of both water and soil has negative impact on the production of rice. The increasing level of sodium ions in the agricultural lands is becoming a severe threat for the agriculture worldwide. The plants suffer an osmotic stress due to the accumulation of salts at the outer side of the roots and suffer an ionic stress due to the accumulation of salts at the inner side of the plants [4]. The increase in food supply must be equal to the rate of increasing population and this demand can only be satisfied via utilizing all the available resources of land at their maximum potential. So, it is also necessary to use the saline soils at their fullest production potential. Different methods like agronomic adjustments, reclamation and different biological amendments are carried out in combination for increasing the production of saline soils. The use of salinity tolerant genetically improved crop varieties is the most suitable option for the sustainable crop production in these areas [62]. The genetic diversity of crops regarding salinity tolerance must be evaluated for developing the salt tolerant crop varieties. The molecular mapping approaches have made it possible to identify the genomic regions responsible for the salt tolerance and assessment of the genetic diversity of different crops and varieties is becoming very easy [63]. The chromosomal regions (QTLs) responsible for the tolerance of salt stress in rice crop can be identified through various molecular mapping approaches.

The salt stress badly effects the physiological, morphological and biochemical features of the rice. It has the negative impact on plant height, shoot dry weight, total tillers, total dry matter and root dry weight. The various physiological attributes that are affected by the salt stress are senescence, calcium ion uptake, sodium ion uptake potassium ion uptake, total cations uptake, osmotic potential, transcription efficiency and relative growth rate [64]. The biochemical features of rice that are effected by the salt stress are proline content, anthocyanins, peroxidase (POX) activity, calcium content, sodium content, potassium content, chlorophyll content and hydrogen peroxide content [4]. Various levels of salinity tolerance are observed at the whole plant level and leaves of rice [65]. Likewise, the rice plant behavior against the salinity stress may vary at reproductive and vegetative phase and this may not be related to the net relative tolerance of the plant. It is mandatory to know the stage that is more susceptible to the salt stress because it is important for comparing the performance of different cultivars during stress. The process of photosynthesis is necessary for the good vegetative and reproductive growth. In the leaf tissues, increased sodium concentration adversely affects the essential cellular metabolism and net photosynthesis. In the process of photosynthesis, chlorophyll content is significantly important but during salinity stress, there is no relationship found between the photosynthesis and chlorophyll content because net photosynthesis rate is decreased by the sodium ion concentration which does not have any connection with the chlorophyll content. It shows disturbance in some other cellular processes of photosynthesis due to salinity stress. The carbon dioxide fixation and stomatal aperture are affected by the sodium ion accumulation in the leaf at the same time, so it can be a reason for the decrease in photosynthesis during salt stress [4]. Different mechanisms have been evolved in rice plant to cope with salt stress conditions. An example of this type of mechanism is compartmenting of salts within the plant.

Numerous genes and QTLs are activated during the salt stress, which could be determined by the different molecular mapping approaches. Many types of molecular marker are present to identify different QTLs. SNP (Single nucleotide polymorphism), SSLP (simple sequence length polymorphism), RFLP (restriction fragment length polymorphism), AFLP (Amplified fragment length polymorphism), STS (sequence tagged sites) and SSRs (simple sequence repeats) are various markers of DNA that are used for genotyping in the studies of molecular mapping [66]. For example: the QTLs of pollen fertility, sodium ion concentration and calcium, sodium and potassium accumulation have been identified in F2 population of rice by using SSR marker [67]. The QTLs for yield related traits and morphological traits are identified by F2 population of rice as plant material and SSR as DNA marker [66] and the QTLs of potassium and sodium uptake for increasing the salinity tolerance in rice are determined by the AFLP, RFLP and SSR [68]. QTLs of salt stress responsive genes have been identified by the SNP in the rice crop [69]. The rice germplasm and these identified QTLs are found salt stress tolerant and are useful for three main reasons: (1) salt tolerance can be understood by the molecular genetics in rice, (2) rice germplasm that is tolerant to salt stress can be introduced to make them salt tolerant and (3) for the screening of rice germplasm, identified QTLs are used against salinity stress [4]. Moreover, new genes responsible for the salt tolerance can be identified and incorporated in the rice cultivars to make them salt tolerant.

2.5 UV radiation stress

The ultraviolet (UV) radiations are present in the region of solar electromagnetic spectrum that has the wavelength (λ) from 200 nm to 400 nm. The UV radiations have shorter wavelength as compared to photosynthetically active radiation that has the wavelength ranges from 700 nm to 400 nm. The UV radiations are composed of three different types that are UV-A, UV-B and UV-C. The UV-C radiations have a very smaller wavelength that is 200-280 nm and it emits photons with high energy which are absorbed totally by ozone layer and are not be able to reach the Earth surface [70]. The UV-A radiations ranges from 315 nm to 415 nm and are more constant. The UV-A radiations causes a little harm to the plants, so the major source of damage are UV-B radiations that have wavelength between 280 and 315 nm [71]. UV-B can cause mutations, reduction in photosynthetic activity, reduction in chlorophyll content, lower electron transfer rate, damage genetic material, decrease the biomass, reduce leaf size, lessen leaf number and eventually decrease the plant productivity. At the cell level, it initiates the oxidative stress through enhancing the level of ROS that ultimately damages the DNA, lipids and proteins; so, the integrity and functionality of cell membrane and enzymes is compromised. The light for photosynthesis can be maximally captured in higher plant by the exposure with UV-B. It is important for increasing the secondary metabolites, enzymatic and non-enzymatic antioxidants, bioactive compounds and cyto-solutes (sugars, glycine, betane, proline) for the survival of plants. The adverse effects of the radiation can be mitigated by the growth regulators present in the plants. The specific signaling pathways are present in plants that are involved in regulating protective gene expression responses against UV-B are vital for survival of plants in sunlight. The identification of the genes responsible for the UV-B radiation, survival is necessary to develop the crop varieties resistant to UV radiation stress [72].

2.6 Heavy metal toxicity

Many solutes are present in the rhizosphere required for the growth and development of plants. Plants uptake these solutes by roots and distribute them in the whole plant body. The successful plant life is ensured by up taking the other components with water from the rhizospheric soil by roots. The developmental plasticity and physiological activity in the plant roots is carried out by the water uptake with soluble elements. The distribution and uptake of these inorganic materials inside the plants is an intrinsic property of energy and material fluidity. In plant cells, a plethora of physiological and structural functions is supported by these essential ions but if these essential ions are present in the non-physiological concentrations, they can turn out as limiting factors. The cellular homeostasis is affected by their availability to plants, Inequalities in comparative abundance of these elements in soil and their rate of uptake. The defense system and adaption of plants is dependent on the developmental and physiological changes triggered by ion toxicity; but it can cause the permanent damage to the plant. In the rhizospheric soil, the ions of heavy metals are also present that can be absorbed by the roots with the water and nutrients and can be incorporated into the plant tissues. The zinc, iron, manganese, copper, aluminum, chromium, cadmium, cobalt, lead, arsenic, nickel and molybdenum are some toxic metals for plants [73].

The concentration of metal ions is excessive in polluted areas and plants growing in those areas suffer from metal toxicity. Some soils have high level of heavy metals naturally like serpentine soils and the mining areas also have high heavy metal content due to activities of human beings. The environmental pollutants including high concentrations of heavy metals are becoming a major challenge for all organisms (plants, animals, microbes, etc.) across the globe. The ionic homeostasis is achieved in plants by maintaining the levels of essential and non-essential ions. Furthermore, this homeostasis in cells and tissues must be maintained in an organ specific manner. The risk of heavy metals can be mitigated by minimizing their uptake, avoiding their exposure and intracellular sequestering in the plants. The cellular structures, membranes structures, basic metabolism and transport processes are affected, if plant is not able to combat with the harmful effects and internalization of heavy metals. The stress reactions are triggered by the high levels of non-toxic and toxic metal ions that disturb the development at molecular, structural and physiological level. The defense mechanism is started in the plants upon the beginning of heavy metal toxicity [74]. The oxidative stress is caused by the heavy metal toxicity, so cells and sub-cellular compartments are protected by plant defense system. The plant growth and development are continued if the heavy metal ions are present in low or moderate concentrations. But, plants cannot survive during the long-term exposure of high level of heavy metal toxicity. The metal tolerance is a unique feature of some plant species in stress biology. The compartmentalization and of heavy metals, restriction of heavy metals in apoplast, extracellular attenuation and chelation of heavy metals and exclusion strategies are some mechanisms of achieving stress tolerance in the plants.

The most practical approach adopted by the plants to combat with the heavy metal toxicity is hyper accumulation and hyper tolerance of heavy metals inside the plant without effecting its viability [75]. The hyper accumulation of metal ions protects the plants from pathogens and herbivores in some conditions (Hörger et al. 2013). The contamination of heavy metals is present in the wide range of terrestrial habitats. In food chain, plants have vital importance, so heavy metal accumulation in plants causes severe contamination in the food chain. So, it is significantly important to know that how plants combat with the heavy metal exposure. The knowledge of these mechanisms is used in developing the practical biological methods to develop the tolerance against heavy metal toxicity [74]. Many promising and existing biotechnological approaches are used are used for this purpose [75]. Before concluding the major efforts used for making plant to heavy metal stress, it is necessary to understand the key factors involved in adjusting the heavy metal tolerance. The total cell protein study is called proteomics; it is a most valuable tool for understanding the basic procedures of development and stress responses in plants [73]. The sensing, transport and uptake of heavy metal, their sequestration in the vacuole and formation of phyto-chelating-heavy metal complex are some main procedures for influencing the plant susceptibility for the metal ions. These processes are controlled at transcriptional and post-transcriptional levels and the secondary process responsible for maintaining the plant during metal toxicity includes the defense reaction derived by the production of antioxidants to preserve the membrane lipid integrity. Diverse genes are responsible for controlling these processes. The heavy metal tolerance of the plants is achieved by the various biotechnological, molecular and genetic tools [74].


3. Rice biotechnology under climate change conditions

Due to climate change, the food availability is affected in 21st century to feed the population which is increasing day by day. Globally, climate change have bad effects on the human health, food security and water resources [76]. About 30-60% of rice income is decreased per year because of different types of stresses [77]. The rice fields are susceptible due to climate change, expansion of urban area and ruined of cultivation field. Due to some cases, 100% rice production is destroy [78]. The rice yield is reduced due to increase in temperature by accumulation of carbon dioxide in environment. The production and quality of rice (Oryza sativa L.) is badly affected by climate change which is mainly abiotic stresses (heat, flood, salinity, cold and drought) and biotic stresses (pest, weeds and pathogens). Climate change cause the formation of unripe grains, white grains, and fractured grains. The grain damage is reduced by use of thermo-stable verities which are formed through conventional breeding and genetic engineering. Thermo tolerant 1 (TT1) is the most important (QTL) present in Oryza glaberrima, it codes α2 which is the subunit of 26S proteasome and is involved in destruction of ubiquitinated protein.

The removal of cytotoxic degraded protein is done by OgTT1 (Oryza glaberrima thermo tolerant 1) maintain the cell from high temperature. Over expression of TT1 increase the ability of heat tolerant stress in rice. It helps to grow the rice in global warming [79]. To protect the food, production of heat tolerant varieties which can be grown in high temperature is a major challenge. In Arabidopsis thaliana, Dehydration responsive element binding protein 2A (DREB2A) is regulated by DNA polymerase subunit B3-1 (DPB3-1), is a transcriptional controller which regulate the growth of plant and increase the thermostability [80]. If temperature is round about 35°C in chamber trial at the time of flowering, then spikelet infertility occurs due to heat stress. When the temperature in Japan cities (Tokai, Kanto) was reached at 40°C in 2007, it induced extensive unproductiveness. The increase in world temperature is expected, that is why we need to develop techniques to protect the grain quality and production from heat damage [81]. During the ripening of rice, the high temperature causes the poor-quality grain production. Late transplanting and thermo-stable varieties are used in preventive measure. Deep-flood irrigation is a process which falling the rate of milky white seeds when the temperature is high and low. Adequate quantity of nitrogen is necessary to protect the grain from disease at reproductive stage [81]. In future, if water availability is less, yield of rice will badly be affected.

Due to climate change, the crop rotation time can be reduced. There are many positive and negative effects of climate change on crop, so scientist try to develop the verities which can be cultivated in harsh conditions to provide enough food to global population [82]. Galactinol synthase 2 gene from Arabidopsis is introduced in two rice verities and have ability to produce the good quality in waterless field environment. The biotechnologically advanced verities have greater intensity of galactinol as compared to non-transgenic verities. The drought resistance rice has greater ability of photosynthesis, recovering and plant growth. It is clear that AtGolS2 gene is beneficial which provide higher quantity of rice in drought stress [83]. Rice varieties which are better grown in anaerobic environment produce less yield in aerobic situation. To accurately measure the expression of different genes in a condition, it is necessary to find out the genes which have same output in aerobic and anaerobic systems [84]. Transcriptional reaction and ecological variation is affected by macroclimate and meadow type, then we identify the genes which are involved in production and photosynthesis [82].


4. Future prospects

In modern-day agriculture, biotechnology has a wide range of applications. It helps in understanding the genetics of important traits as well as in developing new cultivars. The transgenic crop deployment and the marker assisted breeding are the two main areas. The remarkable progress has been made by the marker assisted rice breeding. Modern tools of biotechnology help in studying the functions of genes and mechanism of resistance through improved varieties via MAS. Exploitation of tissue culture techniques used as a vital mean or work as an assistant to other procedures together with recombinant DNA methods is at its frontline in plant improvement and modification for agriculture. The targeted genome editing methods like ZFNs, TALENs, CRISPR/Cas9 are implanted for the modification of non-operational alleles of the useful genes or to repress or knock out the non-useful alleles in plants without making the plant transgenic. The techniques and tools of biotechnology are a very sophisticated option for the rice breeders to develop the useful crop varieties.



This work was supported by funds from Higher Education Commission of Pakistan.


  1. 1. Wani SH, Sah SK. Biotechnology and abiotic stress tolerance in rice. J. Rice Res. 2014;2:e105
  2. 2. Clement WK, Wong MY, Jugah K, Maziah M. Producing Transgenic Rice with Improved Traits and Yield–How Far Have We Come? Pertanika J. Schol. Res. Rev. 2017;3(3)
  3. 3. Hiei Y, Ohta S, Komari T, Kumashiro T. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal. 1994;6(2):271-282
  4. 4. Saeed M. Abiotic stress tolerance in Rice (Oryza sativa L.): a genomics perspective of salinity tolerance. Developments. 2018;5:181
  5. 5. Todaka D, Shinozaki K, Yamaguchi-Shinozaki K. Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants. Frontiers in Plant Science. 2015;6:84
  6. 6. Agarwal M, Sahi C, Katiyar-Agarwal S, Agarwal S, Young T, Gallie DR, et al. Molecular characterization of rice hsp101: Complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types. Plant Molecular Biology. 2003;51(4):543-553
  7. 7. Jeong MJ, Lee SK, Kim BG, Kwon TR, Cho WS, Park YT, et al. A rice (Oryza sativa L.) MAP kinase gene, OsMAPK44, is involved in response to abiotic stresses. Plant Cell, Tiss Org Cult. 2006;85(2):151-160
  8. 8. Lian HL, Yu X, Ye Q , Ding XS, Kitagawa Y, Kwak SS, et al. The role of aquaporin RWC3 in drought avoidance in rice. Plant & Cell Physiology. 2004;45(4):481-489
  9. 9. Shirasawa K, Takabe T, Takabe T, Kishitani S. Accumulation of glycinebetaine in rice plants that overexpress choline monooxygenase from spinach and evaluation of their tolerance to abiotic stress. Annals of Botany. 2006;98(3):565-571
  10. 10. Zhao FY, Zhang XJ, Li PH, Zhao YX, Zhang H. Co-expression of the Suaeda salsa SsNHX1 and Arabidopsis AVP1 confer greater salt tolerance to transgenic rice than the single SsNHX1. Molecular Breeding. 2006;17(4):341-353
  11. 11. Feng L, Wang K, Li Y, Tan Y, Kong J, Li H, et al. Overexpression of SBPase enhances photosynthesis against high temperature stress in transgenic rice plants. Plant Cell Reports. 2007;26(9):1635-1646
  12. 12. Oh SJ, Kwon CW, Choi DW, Song SI, Kim JK. Expression of barley HvCBF4 enhances tolerance to abiotic stress in transgenic rice. Plant Biotechnology Journal. 2007;5(5):646-656
  13. 13. Fukao T, Bailey-Serres J. Submergence tolerance conferred by Sub1A is mediated by SLR1 and SLRL1 restriction of gibberellin responses in rice. Proc. Natl. Acad. Sci. USA. 2008;105(43):16814-16819
  14. 14. Wang Q , Guan Y, Wu Y, Chen H, Chen F, Chu C. Overexpression of a rice OsDREB1F gene increases salt, drought, and low temperature tolerance in both Arabidopsis and rice. Plant Molecular Biology. 2008;67(6):589-602
  15. 15. Huang CF, Yamaji N, Mitani N, Yano M, Nagamura Y, Ma JF. A bacterial-type ABC transporter is involved in aluminum tolerance in rice. The Plant Cell. 2009;21(2):655-667
  16. 16. Huang J, Sun SJ, Xu DQ , Yang X, Bao YM, Wang ZF, et al. Increased tolerance of rice to cold, drought and oxidative stresses mediated by the overexpression of a gene that encodes the zinc finger protein ZFP245. Biochemical and Biophysical Research Communications. 2009;389(3):556-561
  17. 17. Kumar V, Shriram V, Kishor PK, Jawali N, Shitole MG. Enhanced proline accumulation and salt stress tolerance of transgenic indica rice by over-expressing P5CSF129A gene. Plant Biotechnol. Rep. 2010;4(1):37-48
  18. 18. Kim SG, Kim ST, Wang Y, Kim SK, Lee CH, Kim KK, et al. Overexpression of rice isoflavone reductase-like gene (OsIRL) confers tolerance to reactive oxygen species. Physiologia Plantarum. 2010;138(1):1-9
  19. 19. Ueno D, Yamaji N, Kono I, Huang CF, Ando T, Yano M, et al. Gene limiting cadmium accumulation in rice. Proc. Natl. Acad. Sci. USA. 2010;107(38):16500-16505
  20. 20. Gaxiola RA, Edwards M, Elser JJ. A transgenic approach to enhance phosphorus use efficiency in crops as part of a comprehensive strategy for sustainable agriculture. Chemosphere. 2011;84(6):840-845
  21. 21. Song SY, Chen Y, Chen J, Dai XY, Zhang WH. Physiological mechanisms underlying OsNAC5-dependent tolerance of rice plants to abiotic stress. Planta. 2011;234(2):331-345
  22. 22. Duan J, Cai W. OsLEA3-2, an abiotic stress induced gene of rice plays a key role in salt and drought tolerance. PLoS One. 2012;7(9):e45117
  23. 23. El-Kereamy A, Bi YM, Ranathunge K, Beatty PH, Good AG, Rothstein SJ. The rice R2R3-MYB transcription factor OsMYB55 is involved in the tolerance to high temperature and modulates amino acid metabolism. PLoS One. 2012;7(12):e52030
  24. 24. Gu JF, Qiu M, Yang JC. Enhanced tolerance to drought in transgenic rice plants overexpressing C4 photosynthesis enzymes. Crop J. 2013;1(2):105-114
  25. 25. Du H, Wu N, Cui F, You L, Li X, Xiong L. A homolog of ETHYLENE OVERPRODUCER, O s ETOL 1, differentially modulates drought and submergence tolerance in rice. The Plant Journal. 2014;78(5):834-849
  26. 26. Xiong H, Li J, Liu P, Duan J, Zhao Y, Guo X, et al. Overexpression of OsMYB48-1, a novel MYB-related transcription factor, enhances drought and salinity tolerance in rice. PLoS One. 2014;9(3):e92913
  27. 27. Chen H, Liu L, Wang L, Wang S, Cheng X. VrDREB2A, a DREB-binding transcription factor from Vigna radiata, increased drought and high-salt tolerance in transgenic Arabidopsis thaliana. Journal of Plant Research. 2016;129(2):263-273
  28. 28. Cai H, Tian S, Dong H, Guo C. Pleiotropic effects of TaMYB3R1 on plant development and response to osmotic stress in transgenic Arabidopsis. Gene. 2015;558(2):227-234
  29. 29. Min HJ, Jung YJ, Kang BG, Kim WT. CaPUB1, a hot pepper U-box E3 ubiquitin ligase, confers enhanced cold stress tolerance and decreased drought stress tolerance in transgenic rice (Oryza sativa L.). Mol. Cells. 2016;39(3):250
  30. 30. Hu T, Zhu S, Tan L, Qi W, He S, Wang G. Overexpression of OsLEA4 enhances drought, high salt and heavy metal stress tolerance in transgenic rice (Oryza sativa L.). Environ. Exp. Bot. 2016;123:68-77
  31. 31. Shen J, Lv B, Luo L, He J, Mao C, Xi D, et al. The NAC-type transcription factor OsNAC2 regulates ABA-dependent genes and abiotic stress tolerance in rice. Scientific Reports. 2017;7:40641
  32. 32. Park SI, Kim YS, Kim JJ, Mok JE, Kim YH, Park HM, et al. Improved stress tolerance and productivity in transgenic rice plants constitutively expressing the Oryza sativa glutathione synthetase OsGS under paddy field conditions. Journal of Plant Physiology. 2017;215:39-47
  33. 33. Li W, Qiang XJ, Han XR, Jiang LL, Zhang SH, Han J, et al. Ectopic expression of a Thellungiella salsuginea aquaporin gene, TsPIP1; 1, increased the salt tolerance of Rice. International Journal of Molecular Sciences. 2018;19(8):2229
  34. 34. Jiang X, Li S, Ding A, Zhang Z, Hao Q , Wang K, et al. The novel rose MYB transcription factor RhMYB96 enhances salt tolerance in transgenic Arabidopsis. Plant Mol. Biol. Rep. 2018;36(3):406-417
  35. 35. Zu X, Lu Y, Wang Q , Chu P, Miao W, Wang H, et al. A new method for evaluating the drought tolerance of upland rice cultivars. Crop J. 2017;5(6):488-498
  36. 36. Saha P, Sade N, Arzani A, Wilhelmi MD, Coe KM, Li B, et al. Effects of abiotic stress on physiological plasticity and water use of Setaria viridis L. Plant Science. 2016;251:128-138
  37. 37. Larkunthod P, Nounjan N, Siangliw JL, Toojinda T, Sanitchon J, Jongdee B, et al. Physiological responses under drought stress of improved drought-tolerant rice lines and their parents. Not. Bot. Horti. Agrobo. 2018;46(2):679-687
  38. 38. Singha DL, Tuteja N, Boro D, Hazarika GN, Singh S. Heterologous expression of PDH47 confers drought tolerance in indica rice. Plant Cell, Tiss. Org. Cult. 2017;130(3):577-589
  39. 39. Zhang CX, Feng BH, Chen TT, Zhang XF, Tao LX, Fu GF. Sugars, antioxidant enzymes and IAA mediate salicylic acid to prevent rice spikelet degeneration caused by heat stress. Plant Growth Regulation. 2017;83(2):313-323
  40. 40. Zafar SA, Hameed A, Khan AS, Ashraf M. Heat shock induced morpho-physiological response in indica rice (Oryza sativa L.) at early seedling stage. Pak. J. Bot. 2017;49(2):453-463
  41. 41. Zafar SA, Hameed A, Nawaz MA, Wei MA, Noor MA, Hussain M. Mechanisms and molecular approaches for heat tolerance in rice (Oryza sativa L.) under climate change scenario. Journal of Integrative Agriculture. 2018;17(4):726-738
  42. 42. Hasanuzzaman M, Nahar K, Alam M, Roychowdhury R, Fujita M. Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. International Journal of Molecular Sciences. 2013;14(5):9643-9684
  43. 43. Scafaro AP, Yamori W, Carmo-Silva AE, Salvucci ME, von Caemmerer S, Atwell BJ. Rubisco activity is associated with photosynthetic thermotolerance in a wild rice (Oryza meridionalis). Physiologia Plantarum. 2012;146(1):99-109
  44. 44. Shamsudin NA, Swamy BM, Ratnam W, Cruz MT, Raman A, Kumar A. Marker assisted pyramiding of drought yield QTLs into a popular Malaysian rice cultivar, MR219. BMC Genetics. 2016;17(1):30
  45. 45. Ye C, Tenorio FA, Argayoso MA, Laza MA, Koh HJ, Redoña ED, et al. Identifying and confirming quantitative trait loci associated with heat tolerance at flowering stage in different rice populations. BMC Genetics. 2015;16(1):41
  46. 46. Wei H, Liu J, Wang Y, Huang N, Zhang X, Wang L, et al. A dominant major locus in chromosome 9 of rice (Oryza sativa L.) confers tolerance to 48 C high temperature at seedling stage. J. Hered. 2013;104(2):287-294
  47. 47. Wu X, Shiroto Y, Kishitani S, Ito Y, Toriyama K. Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter. Plant Cell Reports. 2009;28(1):21-30
  48. 48. Kusumi K, Iba K. Establishment of the chloroplast genetic system in rice during early leaf development and at low temperatures. Frontiers in Plant Science. 2014;5:386
  49. 49. Zhao J, Zhang S, Dong J, Yang T, Mao X, Liu Q , et al. A novel functional gene associated with cold tolerance at the seedling stage in rice. Plant Biotechnology Journal. 2017;15(9):1141-1148
  50. 50. Zhao C, Lang Z, Zhu JK. Cold responsive gene transcription becomes more complex. Trends in Plant Science. 2015;20(8):466-468
  51. 51. Zhu JK. Abiotic stress signaling and responses in plants. Cell. 2016;167(2):313-324
  52. 52. Chen L, Zhao Y, Xu S, Zhang Z, Xu Y, Zhang J, et al. Os MADS 57 together with Os TB 1 coordinates transcription of its target Os WRKY 94 and D14 to switch its organogenesis to defense for cold adaptation in rice. The New Phytologist. 2018;218(1):219-231
  53. 53. Hong JH, Savina M, Du J, Devendran A, Ramakanth KK, Tian X, et al. A sacrifice-for-survival mechanism protects root stem cell niche from chilling stress. Cell. 2017;170(1):102-113
  54. 54. Jiang L, Liu X, Xiong G, Liu H, Chen F, Wang L, et al. DWARF 53 acts as a repressor of strigolactone signalling in rice. Nature. 2013;504(7480):401-405
  55. 55. Yao R, Ming Z, Yan L, Li S, Wang F, Ma S, et al. DWARF14 is a non-canonical hormone receptor for strigolactone. Nature. 2016;536(7617):469-473
  56. 56. Guo S, Xu Y, Liu H, Mao Z, Zhang C, Ma Y, et al. The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14. Nature Communications. 2013;4(1):1-2
  57. 57. Janssen BJ, Drummond RS, Snowden KC. Regulation of axillary shoot development. Current Opinion in Plant Biology. 2014;17:28-35
  58. 58. Liu X, Lan J, Huang Y, Cao P, Zhou C, Ren Y, et al. WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress. Journal of Experimental Botany. 2018;69(16):3949-3961
  59. 59. Ma Q , Dai X, Xu Y, Guo J, Liu Y, Chen N, et al. Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes. Plant Physiology. 2009;150(1):244-256
  60. 60. Su CF, Wang YC, Hsieh TH, Lu CA, Tseng TH, Yu SM. A novel MYBS3-dependent pathway confers cold tolerance in rice. Plant Physiology. 2010;153(1):145-158
  61. 61. Sanghera GS, Wani SH, Hussain W, Singh NB. Engineering cold stress tolerance in crop plants. Current Genomics. 2011;12(1):30
  62. 62. Singh R, Singh Y, Xalaxo S, Verulkar S, Yadav N, Singh S, et al. From QTL to variety-harnessing the benefits of QTLs for drought, flood and salt tolerance in mega rice varieties of India through a multi-institutional network. Plant Science. 2016;242:278-287
  63. 63. Khan MS, Saeed M, Iqbal J. Association mapping validates previously identified quantitative trait loci for salt tolerance in rice (Oryza sativa L.). Mol. Breed. 2016;36(12):172
  64. 64. Negrão S, Schmöckel SM, Tester M. Evaluating physiological responses of plants to salinity stress. Annals of Botany. 2017;119(1):1-1
  65. 65. Sytar O, Brestic M, Zivcak M, Olsovska K, Kovar M, Shao H, et al. Applying hyperspectral imaging to explore natural plant diversity towards improving salt stress tolerance. Sci. Total Environ. 2017;578:90-99
  66. 66. Khan MS, Saeed M, Iqbal J. Quantitative trait locus mapping for salt tolerance at maturity stage in indica rice using replicated F 2 population. Braz. J. Bot. 2016;39(2):641-650
  67. 67. Khan MS, Saeed M, Iqbal J. Identification of quantitative trait loci for Na+, K+ and Ca++ accumulation traits in rice grown under saline conditions using F 2 mapping population. Braz. J. Bot. 2015;38(3):555-565
  68. 68. Koyama ML, Levesley A, Koebner RM, Flowers TJ, Yeo AR. Quantitative trait loci for component physiological traits determining salt tolerance in rice. Plant Physiology. 2001;125(1):406-422
  69. 69. Kumar V, Singh A, Mithra SA, Krishnamurthy SL, Parida SK, Jain S, et al. Genome-wide association mapping of salinity tolerance in rice (Oryza sativa). DNA Research. 2015;22(2):133-145
  70. 70. Vishwakarma K, Upadhyay N, Kumar N, Yadav G, Singh J, Mishra RK, et al. Abscisic acid signaling and abiotic stress tolerance in plants: A review on current knowledge and future prospects. Frontiers in Plant Science. 2017;8:161
  71. 71. Singh SS, KUMAR P, RA AK. Ultraviolet radiation stress: Molecular and physiological adaptations in trees. In: Abiotic stress tolerance in plants 2006 (pp. 91-110). Springer. Dordrecht
  72. 72. Gadi BR. Effect of UV - B radiation on plants. IJSRST. 2018;4:255-260
  73. 73. Hossain Z, Komatsu S. Contribution of proteomic studies towards understanding plant heavy metal stress response. Front. Plant Sc. 2013;3:310
  74. 74. Ovečka M, Takáč T. Managing heavy metal toxicity stress in plants: Biological and biotechnological tools. Biotechnology Advances. 2014;32(1):73-86
  75. 75. DalCorso G, Manara A, Furini A. An overview of heavy metal challenge in plants: From roots to shoots. Metallomics. 2013;5(9):1117-1132
  76. 76. Kang Y, Khan S, Ma X. Climate change impacts on crop yield, crop water productivity and food security–A review. Progress in Natural Science. 2009;19(12):1665-1674
  77. 77. Sharma I, Kaur N, Pati PK. Brassinosteroids: A promising option in deciphering remedial strategies for abiotic stress tolerance in rice. Frontiers in Plant Science. 2017;8:2151
  78. 78. Mottaleb KA, Rejesus RM, Murty MV, Mohanty S, Li T. Benefits of the development and dissemination of climate-smart rice: Ex ante impact assessment of drought-tolerant rice in South Asia. Mitig. Adapt. Strat. Gl. 2017;22(6):879-901
  79. 79. Li XM, Chao DY, Wu Y, Huang X, Chen K, Cui LG, et al. Natural alleles of a proteasome α2 subunit gene contribute to thermotolerance and adaptation of African rice. Nature Genetics. 2015;47(7):827-833
  80. 80. Sato H, Todaka D, Kudo M, Mizoi J, Kidokoro S, Zhao Y, et al. The A rabidopsis transcriptional regulator DPB 3-1 enhances heat stress tolerance without growth retardation in rice. Plant Biotechnology Journal. 2016;14(8):1756-1767
  81. 81. Morita S, Wada H, Matsue Y. Countermeasures for heat damage in rice grain quality under climate change. Plant Prod. Sci. 2016;19(1):1-1
  82. 82. Plessis A, Hafemeister C, Wilkins O, Gonzaga ZJ, Meyer RS, Pires I, et al. Multiple abiotic stimuli are integrated in the regulation of rice gene expression under field conditions. eLife. 2015;4:e08411
  83. 83. Abdullah E, Idris A, Saparon A. Papr reduction using scs-slm technique in stfbc mimo-ofdm. ARPN J. Eng. Appl. Sci. 2017;12(10):3218-3221
  84. 84. Phule AS, Barbadikar KM, Madhav MS, Senguttuvel P, Babu MP, Kumar PA. Genes encoding membrane proteins showed stable expression in rice under aerobic condition: novel set of reference genes for expression studies. 3 Biotech. 2018;8(9):383

Written By

Munazza Ijaz, Roshina Shahzadi, Akmaral U. Issayeva, Shazia Anwer Bukhari and Mahmood-ur-Rahman

Submitted: 21 July 2020 Published: 03 March 2021