Sisal (Agave sisalana) is one of the main sources of hard natural fibre and raw materials for the industry, medicine and handicrafts. Sisal yields a coarse and strong fibre that is increasingly being used in composite materials for automobiles, furniture, construction and plastic and paper products. Extracts of sisal contain substances with anti-inflammatory, antimicrobial and anthelmintic activities. Sisal is adapted to warm environments with low rainfall and is an excellent option for cultivation in semiarid conditions, where other crops cannot be grown. The world’s largest sisal producers are Brazil, Tanzania, China, Kenya and Madagascar. Sisal is a labour-intensive crop with great socio-economical importance as it is cultivated in poor areas employing familiar labour. Sisal bole rot is the main disease of sisal, responsible for substantial losses in producing countries. The disease is caused by certain species of the genus Aspergillus, especially the ones belonging in the section Nigri. The main symptoms are yellowing of the aerial parts and the red-coloured rot of the bole, which causes the plant to die. In this review we are going to address the taxonomy of the causal agents, disease diagnosis and epidemiology and disease management, with emphasis on biological control.
- Aspergillus welwitschiae
- Agave sisalana
- biological control
- disease management
- semiarid regions
Sisal is a monocarpic plant, and the emission of an inflorescence characterises the end of its vegetative cycle, which can occur between 8 and 30 years. The plant multiplies vegetatively through bulbils produced on the inflorescence pole or by stolons that emerge from the rhizome (subterraneous stem) of adult plants. The use of bulbils is the most common form of propagation, but stolons can also be used. The production of seeds is rare, and induction techniques are necessary when this is the objective [8, 9, 10]. Most species of
There has been a growing interest in the use of waste or by-products from
The main world producers of sisal fibre are Brazil, Tanzania, China, Kenya and Madagascar . Other countries, such as Mexico, South Africa, Mozambique, Angola, Indonesia, Thailand, Haiti and Cuba, also produce but in smaller quantities. According to FAO reports, in 2011 Brazil alone produced more than 111 thousand tons of sisal fibre .
In Brazil, the semiarid region of Bahia province (northeastern Brazil) is responsible for more than 95% of the country’s sisal production . Other provinces that produce smaller amounts of sisal in Brazil are Paraiba, Rio Grande do Norte and Ceará . It is estimated that more than 150,000 families are directly linked to the producing chain of this crop, totalling more than 700,000 small farmers, and more than half a million direct and indirect jobs are involved in activities related to the maintenance, harvesting, extraction and processing of fibre [28, 29, 30]. In this sense, sisal has an important economic and social role of the semiarid region of Brazil.
Sisal management is simple because this plant exhibits tolerance to various abiotic stresses. Even under minimal management conditions, the plant presents good development and consequently good fibre production, with low nutritional requirements . However, although it presents all these adaptive advantages to stress conditions, the main problem is of phytosanitary origin. Sisal bole rot, the main disease of sisal, has caused considerable damage to the crop . This disease causes the death of infected plants, and despite the economic and social importance of sisal, there are few government efforts to control the disease.
In this chapter we introduce the sisal bole rot disease, a neglected disease that represents the main challenge for sisal production in Brazil and other countries of the world. In addition, we discuss some aspects involved in its symptomatology, aetiology, epidemiology and management. The majority of the results that will be shown were obtained in Brazil, where most of the research on sisal bole rot was done.
2. Bole rot disease: symptoms and epidemiology
The disease was first reported in production areas of Tanzania and Brazil [31, 32]. In Brazil, since the 1990s, the commercial production of sisal has been declining due to economical crises and the occurrence of this disease . Diseased plants produce leaves that are not suitable for fibre extraction as they lose their turgescence, and although these diseased plants survive for some time, they die with the progress of the disease (Figure 1) . Plants at advanced stages of the disease are easily identified by the symptoms, which include wilting and yellowing of the aerial part (Figure 1A). The main internal symptom of the disease is rotting of the stem with reddening of the tissues, a response of the plant to fungal colonisation. It is thought that there is no relationship between the phenological stage of the plant and the establishment of the disease, since the fungus is capable of infecting both plantlets (Figure 2E) and adult plants (Figures 1 and 2).
It was reported that the pathogen depends on mechanical injuries and natural openings, mainly on physiologically stressed plants, to start the infection process . In this sense, it is possible that wounds made by insects or by tools used in crop management, such as harvest of the leaves and cultural practices, are ways of pathogen penetration [32, 35, 36]. The histopathology of diseased plants showed that the pathogen penetrates the tissues of the host from the outside, that is, from the epidermis to the parenchyma and later to the central cylinder of the plant .
Abreu  studied the spatiotemporal distribution of sisal bole rot in producing areas of Bahia Province, Brazil, and found that the disease was present in all the studied farms (prevalence of 100%) and, on average, 35% of the plants were infected by the pathogen. This study also showed that the distribution of the disease occurs randomly in the cultivated areas . In the case of sisal bole rot, incidence evaluations are more important than severity, as there are no measures that slow down the progress of the disease.
The lack of more studies on epidemiological aspects of sisal bole rot in different areas where the disease occurs directly impacts the establishment of phytosanitary management practices. More information on these aspects could contribute to the development of strategies to reduce the incidence of the disease. For the moment, what is known is that preventive measures should be employed to avoid the establishment of the pathogen in the area.
3. Causal agents
The disease was first observed in areas of sisal production in Tanzania in the 1930s but was only reported in the 1950s . The causal agent was isolated from diseased plant parts and identified as
In Tanzania and in Brazil, the disease was initially associated with the species
The polyphasic taxonomy integrates molecular, physiological, metabolite production and morphological data for the identification and description of new species of the section
The initial studies implicated only
4. Disease management
There are no effective control methods available for bole rot disease . Mechanical lesions are used by the pathogen as penetration sites, and this has direct implications for crop management since leaf harvest causes wounds in the plant [32, 36]. Additionally, the pathogen may be spread through the use of tools contaminated in diseased plants.
Most farmers use plantlets from stolons to establish new plantations, and infected plant material contributes to the spread of the disease to new areas. Therefore, the establishment of new areas using healthy plant material is thought to be one of the most effective ways to prevent the introduction of the pathogen. Removal and destruction of diseased plants from the plantations, balanced fertilisation to prevent stresses and disinfestation of the tools used in diseased plants are other measures recommended to decrease the incidence and avoid the spread of the disease to new areas .
Another method investigated to manage the disease is the use of antagonistic microorganisms [53, 34]. Chemical control was never investigated probably because the causal agents are soilborne fungi and farmers have little financial resources. Biological control is an environmentally friendly and viable method to control plant pathogens [54, 45]. Antagonistic bacteria were shown to have potential to control the bole rot disease [53, 34]. Several strains of an undescribed species of
Little is known about the mechanisms used by the pathogen to infect the plant, although
Sisal bole rot cannot be controlled by any single method, and therefore, the integration of control measures must be adopted. Resistant cultivars are not available for this crop, and unfortunately there are no breeding programmes focusing on sisal bole rot . Breeding programmes are limited by the low genetic diversity of natural populations out of Mexico.
Preventive measures are thought to be the most effective ways to control bole rot, and these include (i) the use of healthy planting material, (ii) balanced fertilisation to avoid nutritional stresses and (iii) maintaining adequate soil humidity levels to avoid physiological imbalances . When these measures are not able to contain the pathogen, removal of diseased plants is recommended to decrease the source of inoculum of the pathogen . One challenge in this regard is the development strategies to identify diseased plants before the dispersal of pathogen propagules.
Sisal residues are commonly used to fertilise plants in the field , but only the fermented residue is suitable for this purpose as fresh residues stimulate the spread of the pathogen . Information such as these could be disseminated to farmers to contribute to the management of the disease. Sisal farmers in many parts of the world do not have access to information on the technical aspects of sisal, depend on familiar labour and have little financial resources to invest in the crop. The information generated so far on the management of the disease through the use of antagonistic bacteria are promising, but it is still necessary to develop it into products that can be used by the farmers. New studies aiming at formulating and distributing biological products should be encouraged to contribute to the sustainability of this crop in the long run. The general lack of research on bole rot classifies it as a neglected disease that deserves more attention from research institutes and the government.
The authors thank the Brazilian agencies CNPq and CAPES for the financial support.