Overview of the sample.
\\n\\n
IntechOpen was founded by scientists, for scientists, in order to make book publishing accessible around the globe. Over the last two decades, this has driven Open Access (OA) book publishing whilst levelling the playing field for global academics. Through our innovative publishing model and the support of the research community, we have now published over 5,700 Open Access books and are visited online by over three million academics every month. These researchers are increasingly working in broad technology-based subjects, driving multidisciplinary academic endeavours into human health, environment, and technology.
\\n\\nBy listening to our community, and in order to serve these rapidly growing areas which lie at the core of IntechOpen's expertise, we are launching a portfolio of Open Science journals:
\\n\\nAll three journals will publish under an Open Access model and embrace Open Science policies to help support the changing needs of academics in these fast-moving research areas. There will be direct links to preprint servers and data repositories, allowing full reproducibility and rapid dissemination of published papers to help accelerate the pace of research. Each journal has renowned Editors in Chief who will work alongside a global Editorial Board, delivering robust single-blind peer review. Supported by our internal editorial teams, this will ensure our authors will receive a quick, user-friendly, and personalised publishing experience.
\\n\\n"By launching our journals portfolio we are introducing new, dedicated homes for interdisciplinary technology-focused researchers to publish their work, whilst embracing Open Science and creating a unique global home for academics to disseminate their work. We are taking a leap toward Open Science continuing and expanding our fundamental commitment to openly sharing scientific research across the world, making it available for the benefit of all." Dr. Sara Uhac, IntechOpen CEO
\\n\\n"Our aim is to promote and create better science for a better world by increasing access to information and the latest scientific developments to all scientists, innovators, entrepreneurs and students and give them the opportunity to learn, observe and contribute to knowledge creation. Open Science promotes a swifter path from research to innovation to produce new products and services." Alex Lazinica, IntechOpen founder
\\n\\nIn conclusion, Natalia Reinic Babic, Head of Journal Publishing and Open Science at IntechOpen adds:
\\n\\n“On behalf of the journal team I’d like to thank all our Editors in Chief, Editorial Boards, internal supporting teams, and our scientific community for their continuous support in making this portfolio a reality - we couldn’t have done it without you! With your support in place, we are confident these journals will become as impactful and successful as our book publishing program and bring us closer to a more open (science) future.”
\\n\\nWe invite you to visit the journals homepage and learn more about the journal’s Editorial Boards, scope and vision as all three journals are now open for submissions.
\\n\\nFeel free to share this news on social media and help us mark this memorable moment!
\\n\\n\\n"}]',published:!0,mainMedia:{caption:"",originalUrl:"/media/original/237"}},components:[{type:"htmlEditorComponent",content:'
After years of being acknowledged as the world's leading publisher of Open Access books, today, we are proud to announce we’ve successfully launched a portfolio of Open Science journals covering rapidly expanding areas of interdisciplinary research.
\n\n\n\nIntechOpen was founded by scientists, for scientists, in order to make book publishing accessible around the globe. Over the last two decades, this has driven Open Access (OA) book publishing whilst levelling the playing field for global academics. Through our innovative publishing model and the support of the research community, we have now published over 5,700 Open Access books and are visited online by over three million academics every month. These researchers are increasingly working in broad technology-based subjects, driving multidisciplinary academic endeavours into human health, environment, and technology.
\n\nBy listening to our community, and in order to serve these rapidly growing areas which lie at the core of IntechOpen's expertise, we are launching a portfolio of Open Science journals:
\n\nAll three journals will publish under an Open Access model and embrace Open Science policies to help support the changing needs of academics in these fast-moving research areas. There will be direct links to preprint servers and data repositories, allowing full reproducibility and rapid dissemination of published papers to help accelerate the pace of research. Each journal has renowned Editors in Chief who will work alongside a global Editorial Board, delivering robust single-blind peer review. Supported by our internal editorial teams, this will ensure our authors will receive a quick, user-friendly, and personalised publishing experience.
\n\n"By launching our journals portfolio we are introducing new, dedicated homes for interdisciplinary technology-focused researchers to publish their work, whilst embracing Open Science and creating a unique global home for academics to disseminate their work. We are taking a leap toward Open Science continuing and expanding our fundamental commitment to openly sharing scientific research across the world, making it available for the benefit of all." Dr. Sara Uhac, IntechOpen CEO
\n\n"Our aim is to promote and create better science for a better world by increasing access to information and the latest scientific developments to all scientists, innovators, entrepreneurs and students and give them the opportunity to learn, observe and contribute to knowledge creation. Open Science promotes a swifter path from research to innovation to produce new products and services." Alex Lazinica, IntechOpen founder
\n\nIn conclusion, Natalia Reinic Babic, Head of Journal Publishing and Open Science at IntechOpen adds:
\n\n“On behalf of the journal team I’d like to thank all our Editors in Chief, Editorial Boards, internal supporting teams, and our scientific community for their continuous support in making this portfolio a reality - we couldn’t have done it without you! With your support in place, we are confident these journals will become as impactful and successful as our book publishing program and bring us closer to a more open (science) future.”
\n\nWe invite you to visit the journals homepage and learn more about the journal’s Editorial Boards, scope and vision as all three journals are now open for submissions.
\n\nFeel free to share this news on social media and help us mark this memorable moment!
\n\n\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"6380",leadTitle:null,fullTitle:"Cytotoxicity",title:"Cytotoxicity",subtitle:null,reviewType:"peer-reviewed",abstract:"The book Cytotoxicity is aimed to be an essential reading to all medical students, biologists, biochemists and professionals involved in the field of toxicology. This book is a useful and ideal guide for novice researchers interested in learning research methods to study cytotoxic bioactive compounds. The parts of this book describe the replacement and different applications of the cytotoxic agents. All chapters are written by paramount experts in cytotoxicity research. This will hopefully stimulate more research initiatives, funding, and critical insight into the already increasing demand for cytotoxicity researches that have been evidenced worldwide.",isbn:"978-1-78923-431-2",printIsbn:"978-1-78923-430-5",pdfIsbn:"978-1-83881-443-4",doi:"10.5772/intechopen.69919",price:119,priceEur:129,priceUsd:155,slug:"cytotoxicity",numberOfPages:278,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"60d61573c9a66207b8bc54613cac5716",bookSignature:"Tülay Aşkin Çelik",publishedDate:"July 25th 2018",coverURL:"https://cdn.intechopen.com/books/images_new/6380.jpg",numberOfDownloads:22763,numberOfWosCitations:40,numberOfCrossrefCitations:33,numberOfCrossrefCitationsByBook:1,numberOfDimensionsCitations:64,numberOfDimensionsCitationsByBook:1,hasAltmetrics:0,numberOfTotalCitations:137,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"June 14th 2017",dateEndSecondStepPublish:"July 5th 2017",dateEndThirdStepPublish:"October 1st 2017",dateEndFourthStepPublish:"December 30th 2017",dateEndFifthStepPublish:"February 28th 2018",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"74041",title:"Dr.",name:"Tulay",middleName:null,surname:"Askin Celik",slug:"tulay-askin-celik",fullName:"Tulay Askin Celik",profilePictureURL:"https://mts.intechopen.com/storage/users/74041/images/system/74041.png",biography:"Dr. Tülay AŞKIN ÇELİK gained her PhD from Fırat University, Art and Science Faculty, Department of Biology, Elazığ/TURKEY. Presently she is an Associate Professor at Aydın Adnan Menderes University, Art and Science Faculty, Department of Biology, Aydın/TURKEY in the field of genetics. She has been a referee for more than 25 international scientific journals and she worked as a researcher and project manager in 13 projects. She is a referent for issues related to the fields of genetic toxicology and anticancer and antioxidant plants. In 2008, she became a member of the European Association for Cancer Research (EACR) and Molecular Cancer Research Association (MOKAD) and in 2018, she became a member of the Medical Biology and Genetics Association (TBGDER). Currently, her scientific interests include bioactive phytochemicals and plant extracts on their cytogenetic and gentotoxic effects on chromosomes and cancer cells and in vivo /in vitro biological activities. Furthermore, she is also investigating the genotoxic and cytotoxic effects of environmental pollutants such as pesticides. She has authored one book and two book chapters in reputed books published by IntechOpen Access Publisher.",institutionString:"Aydın Adnan Menderes University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"3",totalChapterViews:"0",totalEditedBooks:"2",institution:{name:"Adnan Menderes University",institutionURL:null,country:{name:"Turkey"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"1208",title:"Medical Toxicology",slug:"medical-toxicology"}],chapters:[{id:"61438",title:"Introductory Chapter: Cytotoxicity",doi:"10.5772/intechopen.77244",slug:"introductory-chapter-cytotoxicity",totalDownloads:1363,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:null,signatures:"Tülay Aşkin Çelik",downloadPdfUrl:"/chapter/pdf-download/61438",previewPdfUrl:"/chapter/pdf-preview/61438",authors:[{id:"74041",title:"Dr.",name:"Tulay",surname:"Askin Celik",slug:"tulay-askin-celik",fullName:"Tulay Askin Celik"}],corrections:null},{id:"57702",title:"Cytotoxicity Caused by Asbestos Fibers and Acquisition of Resistance by Continuous Exposure in Human T Cells",doi:"10.5772/intechopen.72064",slug:"cytotoxicity-caused-by-asbestos-fibers-and-acquisition-of-resistance-by-continuous-exposure-in-human",totalDownloads:991,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The cytotoxic effects of asbestos fibers on human T cells and the acquisition of resistance against asbestos-induced apoptosis have been studied. These analyses are based on the establishment of a continuous and relatively low-dose exposure model of human immune cells exposed to asbestos that resembles actual exposure in the human body. The MT-2 T cell line was selected as the candidate for the investigations. A transient and high-dose exposure to chrysotile resulted in apoptosis with production of reactive oxygen species (ROS) and activation of the mitochondrial apoptotic pathway. However, sublines continuously exposed to low dose of asbestos exhibited resistance to asbestos-induced apoptosis. The mechanism of resistance acquisition involved excess production of IL-10, activation of STAT3, and enhanced expression of Bcl-2 located downstream of STAT3. These changes were also found in a subline continuously exposed to crocidolite. Furthermore, sublines showed a marked decrease in the expression of forkhead box O1 (FoxO1) transcription factor. FoxO1 is known to regulate apoptosis and various other cellular processes. Regarding apoptosis, sublines continuously exposed to asbestos showed reduction of FoxP1-driven proapoptotic genes. This pathway is also considered one of the mechanisms that result in resistance to asbestos-induced apoptosis in sublines. These sublines also exhibited several characteristics suggesting reduction of antitumor immunity.",signatures:"Hidenori Matsuzaki, Suni Lee, Naoko Kumagai-Takei, Yu Min,\nNagisa Sada, Kei Yoshitome, Yasumitsu Nishimura, Megumi Maeda\nand Takemi Otsuki",downloadPdfUrl:"/chapter/pdf-download/57702",previewPdfUrl:"/chapter/pdf-preview/57702",authors:[{id:"34101",title:"Prof.",name:"Takemi",surname:"Otsuki",slug:"takemi-otsuki",fullName:"Takemi Otsuki"},{id:"48627",title:"Dr.",name:"Naoko",surname:"Kumagai-Takei",slug:"naoko-kumagai-takei",fullName:"Naoko Kumagai-Takei"},{id:"48631",title:"Dr.",name:"Yasumitsu",surname:"Nishimura",slug:"yasumitsu-nishimura",fullName:"Yasumitsu Nishimura"},{id:"104893",title:"Dr.",name:"Suni",surname:"Lee",slug:"suni-lee",fullName:"Suni Lee"},{id:"104894",title:"Dr.",name:"Hidenori",surname:"Matsuzaki",slug:"hidenori-matsuzaki",fullName:"Hidenori Matsuzaki"},{id:"219725",title:"Dr.",name:"Kei",surname:"Yoshitome",slug:"kei-yoshitome",fullName:"Kei Yoshitome"},{id:"222581",title:"MSc.",name:"Yu",surname:"Min",slug:"yu-min",fullName:"Yu Min"},{id:"222582",title:"Dr.",name:"Nagisa",surname:"Sada",slug:"nagisa-sada",fullName:"Nagisa Sada"},{id:"222583",title:"Prof.",name:"Megumi",surname:"Maeda",slug:"megumi-maeda",fullName:"Megumi Maeda"}],corrections:null},{id:"58055",title:"The Cytotoxic, Antimicrobial and Anticancer Properties of the Antimicrobial Peptide Nisin Z Alone and in Combination with Conventional Treatments",doi:"10.5772/intechopen.71927",slug:"the-cytotoxic-antimicrobial-and-anticancer-properties-of-the-antimicrobial-peptide-nisin-z-alone-and",totalDownloads:7279,totalCrossrefCites:7,totalDimensionsCites:11,hasAltmetrics:0,abstract:"Nisin is an antimicrobial peptide commonly used as a food preservative since 1969. This peptide has potent antimicrobial activity against several Gram-positive bacterial strains, including clinically important and resistant pathogens. The combination of nisin with conventional antibiotics has been shown to improve the antimicrobial activity of these antibiotic agents. Apart from the antimicrobial properties of nisin, this AMP also displays promising anticancer potential towards several types of malignancies. The nisin Z variant is able to induce selective cytotoxicity in melanoma cells compared to non-malignant cells. It was shown that nisin Z disrupts the cell membrane integrity of melanoma cells and that cytotoxicity is likely due to the activation of an apoptotic pathway. In addition, when used in combination with the conventional chemotherapeutic agents, nisin Z has the potential to enhance the cytotoxicity of these chemotherapeutic agents against cultured melanoma cells. Nisin Z has great potential for clinical application considering its low cytotoxicity to non-malignant cells and its effectiveness against Gram-positive bacterial strains and certain cancers.",signatures:"Angélique Lewies, Lissinda H. Du Plessis and Johannes F. Wentzel",downloadPdfUrl:"/chapter/pdf-download/58055",previewPdfUrl:"/chapter/pdf-preview/58055",authors:[{id:"214707",title:"Dr.",name:"Johannes",surname:"Wentzel",slug:"johannes-wentzel",fullName:"Johannes Wentzel"},{id:"216091",title:"Dr.",name:"Angélique",surname:"Lewies",slug:"angelique-lewies",fullName:"Angélique Lewies"},{id:"216092",title:"Prof.",name:"Lissinda",surname:"Du Plessis",slug:"lissinda-du-plessis",fullName:"Lissinda Du Plessis"}],corrections:null},{id:"58788",title:"Cytotoxic Colchicine Alkaloids: From Plants to Drugs",doi:"10.5772/intechopen.72622",slug:"cytotoxic-colchicine-alkaloids-from-plants-to-drugs",totalDownloads:1445,totalCrossrefCites:2,totalDimensionsCites:4,hasAltmetrics:0,abstract:"Plants produce and store many organic compounds like amino acids, proteins, carbohydrates, fats, and alkaloids, which are usually treated as secondary metabolites. Many alkaloids are biologically active for humans. For thousand years, extracts from plants containing alkaloids had medicinal use as drugs and they owe their powerful effects thanks to presence of alkaloids. Alkaloids have anti-inflammatory, antibacterial, analgesic, local anesthetic, hypnotic, psychotropic, antimitotic, and antitumor activity. Nowadays, alkaloids from plants are still of great interest to organic chemists, pharmacologists, biologists, biochemists, and pharmacists. Plants of Liliaceae family contain colchicine as the main alkaloid, which has cytotoxic activity. Colchicine has limited pharmacological application because of its toxicity, but many derivatives have been synthesized and their cytotoxic activity and tubulin-binding properties have been tested. Many of the synthetic derivatives showed good cytotoxic activity.",signatures:"Joanna Kurek",downloadPdfUrl:"/chapter/pdf-download/58788",previewPdfUrl:"/chapter/pdf-preview/58788",authors:[{id:"214632",title:"Dr.",name:"Joanna",surname:"Kurek",slug:"joanna-kurek",fullName:"Joanna Kurek"}],corrections:null},{id:"58539",title:"Natural Products as Cytotoxic Agents in Chemotherapy against Cancer",doi:"10.5772/intechopen.72744",slug:"natural-products-as-cytotoxic-agents-in-chemotherapy-against-cancer",totalDownloads:1499,totalCrossrefCites:6,totalDimensionsCites:8,hasAltmetrics:0,abstract:"Nature continues to produce a great wealth of natural molecules endowed with cytotoxic activity toward a large panel of tumor cells. Some of these molecules are used in chemotherapy, and others have shown great anti-tumor and anti-metastatic potential in preclinical trials. This review discusses some examples of these molecules that have been studied in our laboratory and others. We report a differential cytotoxic activity of some monoterpenes (carvacrol, tymol, carveol, carvone, and isopulegol) against a panel of tumor cell lines. The carvacrol was the most cytotoxic molecule both in vitro and in vivo as demonstrated by preclinical studies using the DBA2/P815 mice model. On the other hand, polyphenols were also studied with respect to their cytotoxic effects. Interestingly, these compounds showed a prominent cytotoxic activity toward a panel of cancer cells with differential molecular mechanisms. In addition, we report a very strong antitumor efficacy of artemisinin, a sesquiterpen lactone from Artemisia annua, together with an antimetastatic potential as demonstrated by preclinical experiments. Furthermore, some of the molecular mechanisms involved in these effects are described.",signatures:"Abdelmajid Zyad, Inass Leouifoudi, Mounir Tilaoui, Hassan Ait\nMouse, Mouna Khouchani and Abdeslam Jaafari",downloadPdfUrl:"/chapter/pdf-download/58539",previewPdfUrl:"/chapter/pdf-preview/58539",authors:[{id:"187827",title:"Dr.",name:"Inass",surname:"Leouifoudi",slug:"inass-leouifoudi",fullName:"Inass Leouifoudi"},{id:"204146",title:"Prof.",name:"Abdelmajid",surname:"Zyad",slug:"abdelmajid-zyad",fullName:"Abdelmajid Zyad"},{id:"216446",title:"Prof.",name:"Hassan",surname:"Ait Mouse",slug:"hassan-ait-mouse",fullName:"Hassan Ait Mouse"},{id:"216447",title:"Prof.",name:"Mouna",surname:"Khouchani",slug:"mouna-khouchani",fullName:"Mouna Khouchani"}],corrections:null},{id:"58323",title:"Cytotoxicity and Apoptosis Induction by Coumarins in CLL",doi:"10.5772/intechopen.72446",slug:"cytotoxicity-and-apoptosis-induction-by-coumarins-in-cll",totalDownloads:1175,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Chronic lymphocytic leukemia (CLL) is one of the leukemia types. Leukemia is cancer of the body's blood-forming cells. Cancer is a disease that is often characterized by too little apoptosis and uncontrolled duplicate of body cells. Apoptosis, or programmed cell death, is a normal component of the development and health of multicellular organisms. Cells die in response to a variety of stimuli during apoptosis. During cancer, pathophysiology apoptosis of the cancerous cells is disrupted, so one of the strategies for cancer chemotherapy is inducing apoptosis in cancerous cells. Myeloid cell leukemia type 1 (Mcl-1) is one of the antiapoptotic Bcl-2 family proteins. It has been shown that the expression of Mcl-1 in CLL is significantly associated with a failure to achieve complete remission following cytotoxic therapy, so regulation of Mcl-1 expression by coumarins could be one of the mechanisms of CLL chemotherapy. Coumarins consist of a large class of phenolic substances found in plants. Different pharmacologic effects of coumarins were reported. One of these effects is cytotoxicity and apoptosis induction in cancerous cells by coumarins. In this chapter, the cytotoxic activity of coumarins and their role in Mcl-1 regulation are discussed.",signatures:"Omid Gholami",downloadPdfUrl:"/chapter/pdf-download/58323",previewPdfUrl:"/chapter/pdf-preview/58323",authors:[{id:"214865",title:"Dr.",name:"Omid",surname:"Gholami",slug:"omid-gholami",fullName:"Omid Gholami"}],corrections:null},{id:"58914",title:"Cyto(Geno)Toxic Endpoints Assessed via Cell Cycle Bioassays in Plant Models",doi:"10.5772/intechopen.72997",slug:"cyto-geno-toxic-endpoints-assessed-via-cell-cycle-bioassays-in-plant-models",totalDownloads:1028,totalCrossrefCites:2,totalDimensionsCites:5,hasAltmetrics:0,abstract:"Environmental pollution is a matter of great concern. Therefore, researches that aim to access the risk of toxicity of these potential pollutants are welcome in the scientific community. The most common strategy to detect toxic agents is through chemical analysis. However, in the last years, the biological assays are often important for risk assessments. Among the bioassays using living organisms to detect toxicity of a compound, plant models have been highlighted as it is easy to be conducted, has low cost, high sensitivity and presents good correlation with other test systems, including mammals. Besides, it is in accordance with the Toxicology Guidelines for the twenty-first century, which claims for bioassays that could substitute the ones that use animals as models. At cellular level, the cytotoxicity, genotoxicity, and mutagenicity are the parameters determined by the endpoints as mitotic index, DNA fragmentation, induction of cell death, and malfunction of cellular structures leading to chromosome and cell cycle alterations. Each of these endpoints will be presented in details in this chapter.",signatures:"Larissa Fonseca Andrade Vieira and Graciele Lurdes Silveira",downloadPdfUrl:"/chapter/pdf-download/58914",previewPdfUrl:"/chapter/pdf-preview/58914",authors:[{id:"215258",title:"Prof.",name:"Larissa",surname:"Fonseca Andrade Vieira",slug:"larissa-fonseca-andrade-vieira",fullName:"Larissa Fonseca Andrade Vieira"},{id:"215260",title:"M.Sc.",name:"Graciele",surname:"Silveira",slug:"graciele-silveira",fullName:"Graciele Silveira"}],corrections:null},{id:"58235",title:"Role of Cytotoxicity Experiments in Pharmaceutical Development",doi:"10.5772/intechopen.72539",slug:"role-of-cytotoxicity-experiments-in-pharmaceutical-development",totalDownloads:1866,totalCrossrefCites:7,totalDimensionsCites:17,hasAltmetrics:0,abstract:"Through the twentieth century, the road from synthetizing a new drug molecule to become an actual product got longer than ever before. Cytotoxicity assays are a quick way to assess a certain chemical compound’s effects on a given human cell line. The most well-known techniques are the MTT- and the LDH-assays. These tests are cheap, easy to execute, but not very precise and dependent on various environmental factors and also, they show no detail about the time-dependency of the toxic effect. Cytotoxicity experiments are a crucial part of a modern pharmaceutical development process. They are a cheap and safe way to get vital information about a new molecule’s biological attributes focusing on its basic tolerability. These studies not only save human lives and test animals, but they save the time and resources to be spared on a test molecule which is a complete failure having no in vitro safety.",signatures:"Ildikó Bácskay, Dániel Nemes, Ferenc Fenyvesi, Judit Váradi, Gábor\nVasvári, Pálma Fehér, Miklós Vecsernyés and Zoltán Ujhelyi",downloadPdfUrl:"/chapter/pdf-download/58235",previewPdfUrl:"/chapter/pdf-preview/58235",authors:[{id:"215449",title:"Dr.",name:"Ildikó",surname:"Bácskay",slug:"ildiko-bacskay",fullName:"Ildikó Bácskay"},{id:"215453",title:"Dr.",name:"Dániel",surname:"Nemes",slug:"daniel-nemes",fullName:"Dániel Nemes"},{id:"215454",title:"Dr.",name:"Zoltán",surname:"Ujhelyi",slug:"zoltan-ujhelyi",fullName:"Zoltán Ujhelyi"},{id:"215456",title:"Dr.",name:"Ferenc",surname:"Fenyvesi",slug:"ferenc-fenyvesi",fullName:"Ferenc Fenyvesi"},{id:"215457",title:"Dr.",name:"Judit",surname:"Váradi",slug:"judit-varadi",fullName:"Judit Váradi"},{id:"215458",title:"Dr.",name:"Gábor",surname:"Vasvári",slug:"gabor-vasvari",fullName:"Gábor Vasvári"},{id:"215459",title:"Dr.",name:"Pálma",surname:"Fehér",slug:"palma-feher",fullName:"Pálma Fehér"},{id:"215460",title:"Dr.",name:"Miklós",surname:"Vecsernyés",slug:"miklos-vecsernyes",fullName:"Miklós Vecsernyés"}],corrections:null},{id:"61672",title:"Biocompatibility of Doped Semiconductors Nanocrystals and Nanocomposites",doi:"10.5772/intechopen.77197",slug:"biocompatibility-of-doped-semiconductors-nanocrystals-and-nanocomposites",totalDownloads:1108,totalCrossrefCites:6,totalDimensionsCites:9,hasAltmetrics:0,abstract:"Exposure of humans and environment to nanocrystals are inevitable, and nanotoxicological analyses are a requirement. The wide variety of nanocrystals with different applications is increasing, and characterization of their effects after exposure includes their potential toxicity and uses. This review summarizes the characterization of doped nanocrystals and nanocomposites, Ca-doped ZnO, Ag- and Eu-doped ZnO and Ni-doped ZnO NCs, their biocompatibility and applications. This review uncovers how these nanocrystals present desirable biocompatible properties, which can be useful as antitumoral and antimicrobial inducing agents, which differ markedly from toxic properties observed in other general nanocrystals.",signatures:"Anielle Christine Almeida Silva, Mariana Alves Pereira Zóia, Lucas Ian Veloso Correia,\nFernanda Van Petten Vasconcelos Azevedo, Aline Teodoro de\nPaula, Larissa Prado Maia, Layara Santana de Carvalho, Loyna\nNobile Carvalho, Maria Paula Camargo Costa, Layssa Carrilho\nGiaretta, Renata Santos Rodrigues, Veridiana de Melo Ávila, Luiz\nRicardo Goulart and Noelio Oliveira Dantas",downloadPdfUrl:"/chapter/pdf-download/61672",previewPdfUrl:"/chapter/pdf-preview/61672",authors:[{id:"13001",title:"Dr.",name:"Noelio",surname:"Oliveira Dantas",slug:"noelio-oliveira-dantas",fullName:"Noelio Oliveira Dantas"},{id:"217672",title:"Prof.",name:"Anielle",surname:"Silva",slug:"anielle-silva",fullName:"Anielle Silva"},{id:"227086",title:"MSc.",name:"Fernanda",surname:"Van Petten Vasconcelos Azevedo",slug:"fernanda-van-petten-vasconcelos-azevedo",fullName:"Fernanda Van Petten Vasconcelos Azevedo"},{id:"227087",title:"MSc.",name:"Mariana",surname:"Alves Pereira Zóia",slug:"mariana-alves-pereira-zoia",fullName:"Mariana Alves Pereira Zóia"},{id:"227089",title:"BSc.",name:"Lucas Ian",surname:"Veloso Correia",slug:"lucas-ian-veloso-correia",fullName:"Lucas Ian Veloso Correia"},{id:"227090",title:"Dr.",name:"Aline",surname:"Teodoro De Paula",slug:"aline-teodoro-de-paula",fullName:"Aline Teodoro De Paula"},{id:"227099",title:"Prof.",name:"Renata",surname:"Santos Rodrigues",slug:"renata-santos-rodrigues",fullName:"Renata Santos Rodrigues"},{id:"227100",title:"Prof.",name:"Veridiana",surname:"De Melo Ávila",slug:"veridiana-de-melo-avila",fullName:"Veridiana De Melo Ávila"},{id:"227101",title:"Prof.",name:"Luiz Ricardo",surname:"Goulart",slug:"luiz-ricardo-goulart",fullName:"Luiz Ricardo Goulart"},{id:"227368",title:"Ms.",name:"Maria Paula",surname:"Camargo Costa",slug:"maria-paula-camargo-costa",fullName:"Maria Paula Camargo Costa"},{id:"227369",title:"Ms.",name:"Layssa",surname:"Carrilho Giaretta",slug:"layssa-carrilho-giaretta",fullName:"Layssa Carrilho Giaretta"}],corrections:null},{id:"57853",title:"Toxicity of Titanate Nanosheets on Human Immune Cells",doi:"10.5772/intechopen.72234",slug:"toxicity-of-titanate-nanosheets-on-human-immune-cells",totalDownloads:951,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Titanium oxide is regarded as a bio-inert material, but studies concerning the toxic effects of titanium dioxide (TiO2), particularly nano-scaled TiO2 particles, have been accumulating that indicate nano-scaled TiO2 particles show more harm and cause greater alteration of immune functions compared with large particles. Inorganic nanosheets have been the focus of increasing interest because of their ultrathin structure, as well as diversity of compounds and structures leading to various functions. Oxide nanosheets are included in the group comprising inorganic nanosheets, and titanate nanosheets (TiNSs) represent a form of oxide nanosheets. We therefore examined the toxicity of nano-scaled 2D materials of TiNSs on human immune cells. Our study revealed that TiNSs have the potential to cause harm through caspase-dependent apoptosis of human peripheral blood mononuclear cells (PBMCs) to the same degree as asbestos. Furthermore, isolated monocytes developed marked vacuoles prior to cell death upon exposure to TiNSs, which were found in the vacuoles and indicated engulfment of TiNSs. A consideration of these findings with the co-localization of vacuoles with endocytosed fluorescence-labeled dextran indicates that TiNSs entered the endosomal pathway, leading to the formation of vacuoles in monocytes and subsequent cell death. TiNSs might therefore affect immune functions through interference of endo-lysosomal functions.",signatures:"Yasumitsu Nishimura, Daisuke Yoshioka, Naoko Kumagai-Takei,\nSuni Lee, Hidenori Matsuzaki, Kei Yoshitome and Takemi Otsuki",downloadPdfUrl:"/chapter/pdf-download/57853",previewPdfUrl:"/chapter/pdf-preview/57853",authors:[{id:"48631",title:"Dr.",name:"Yasumitsu",surname:"Nishimura",slug:"yasumitsu-nishimura",fullName:"Yasumitsu Nishimura"}],corrections:null},{id:"58365",title:"General Cytotoxicity and Its Application in Nanomaterial Analysis",doi:"10.5772/intechopen.72578",slug:"general-cytotoxicity-and-its-application-in-nanomaterial-analysis",totalDownloads:1577,totalCrossrefCites:3,totalDimensionsCites:6,hasAltmetrics:0,abstract:"The recent increasing interest in the use of different nanoparticles in biological and medical applications encouraged scientists to analyse their potential impact on biological systems. The biocompatibility analyses of novel materials for medical applications are conducted using quantitative and qualitative techniques collected by the International Standards Organization (ISO). The well-known assays, such as tetrazolium-based assays used for mitochondrial function monitoring, LDH for membrane permeability determination and neutral red uptake (NRU) describing lysosome function, need to be optimised due to specific properties of wide range of nanomaterials. Physicochemical properties of nanoparticles (NPs) such as size, composition, concentration, shape and surface (e.g., charge, coating, aspect ratio), as well as the cell type play a crucial role in determining the nanomaterial toxicity (also uptake pathway(s) of NPs). Different nanomaterials exhibit different cytotoxicity from relatively non-toxic hexagonal boron nitride to rutile TiO2 NPs that induce oxidative DNA damage in the absence of UV light. Finally, the results of the nanomedical analysis can be enriched by holographic microscopy that gives valuable information about the doubling time (DT), cell segmentation, track cell movement and changes in cell morphology. The results can be also completed by phenotype microarrays (PMs) and atomic force microscopy (AFM) techniques that fulfil experimental data.",signatures:"Magdalena Jedrzejczak-Silicka and Ewa Mijowska",downloadPdfUrl:"/chapter/pdf-download/58365",previewPdfUrl:"/chapter/pdf-preview/58365",authors:[{id:"186478",title:"Dr.",name:"Magdalena",surname:"Jedrzejczak-Silicka",slug:"magdalena-jedrzejczak-silicka",fullName:"Magdalena Jedrzejczak-Silicka"},{id:"231014",title:"Prof.",name:"Ewa",surname:"Mijowska",slug:"ewa-mijowska",fullName:"Ewa Mijowska"}],corrections:null},{id:"61587",title:"Review of In vitro Toxicity of Nanoparticles and Nanorods: Part 1",doi:"10.5772/intechopen.76365",slug:"review-of-in-vitro-toxicity-of-nanoparticles-and-nanorods-part-1",totalDownloads:1122,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"The specific use of engineered nanostructures in biomedical applications has become very attractive, due to their ability to interface and target specific cells and tissues to execute their functions. Additionally, there is continuous progress in research on new nanostructures with unique optical, magnetic, catalytic, and electrochemical properties that can be exploited for therapeutic or diagnostic methods. On the other hand, as nanostructures become widely used in many different applications, the unspecific exposure of humans to them is also unavoidable. Therefore, studying and understanding the toxicity of such materials is of increasing importance. Previously published reviews regarding the toxicological effects of nanostructures focuses mostly on the cytotoxicity of nanoparticles and their internalization, activated signaling pathways, and cellular response. Here, the most recent studies on the in vitro cytotoxicity of NPs, nanowires, and nanorods for biomedical applications are reviewed and divided into two parts. The first part considers nonmagnetic metallic and magnetic nanostructures. While part 2 covers carbon structures and semiconductors. The factors influencing the toxicity of these nanostructures are elaborated, to help elucidating the effects of these nanomaterials on cells, which is a prerequisite for their save clinical use.",signatures:"Jose Efrain Perez, Nouf Alsharif, Aldo Isaac Martínez Banderas,\nBasmah Othman, Jasmeen Merzaban, Timothy Ravasi and Jürgen\nKosel",downloadPdfUrl:"/chapter/pdf-download/61587",previewPdfUrl:"/chapter/pdf-preview/61587",authors:[{id:"141848",title:"Prof.",name:"Jurgen",surname:"Kosel",slug:"jurgen-kosel",fullName:"Jurgen Kosel"},{id:"225868",title:"Mr.",name:"Jose",surname:"Perez",slug:"jose-perez",fullName:"Jose Perez"},{id:"225869",title:"Mrs.",name:"Nouf",surname:"Alsharif",slug:"nouf-alsharif",fullName:"Nouf Alsharif"},{id:"225870",title:"Mr.",name:"Aldo Martinez",surname:"Banderas",slug:"aldo-martinez-banderas",fullName:"Aldo Martinez Banderas"},{id:"225871",title:"Prof.",name:"Jasmeen",surname:"Merzaban",slug:"jasmeen-merzaban",fullName:"Jasmeen Merzaban"},{id:"225872",title:"Prof.",name:"Timothy",surname:"Ravasi",slug:"timothy-ravasi",fullName:"Timothy Ravasi"}],corrections:null},{id:"62231",title:"Review of In Vitro Toxicity of Nanoparticles and Nanorods—Part 2",doi:"10.5772/intechopen.78616",slug:"review-of-in-vitro-toxicity-of-nanoparticles-and-nanorods-part-2",totalDownloads:1362,totalCrossrefCites:0,totalDimensionsCites:2,hasAltmetrics:0,abstract:"The specific use of engineered nanostructures in biomedical applications has become very attractive, due to their ability to interface and target specific cells and tissues to execute their functions. Additionally, there is continuous progress in research on new nanostructures with unique optical, magnetic, catalytic and electrochemical properties that can be exploited for therapeutic or diagnostic methods. On the other hand, as nanostructures become widely used in many different applications, the unspecific exposure of humans to them is also unavoidable. Therefore, studying and understanding the toxicity of such materials are of increasing importance. Previously published reviews regarding the toxicological effects of nanostructures focus mostly on the cytotoxicity of nanoparticles and their internalization, activated signaling pathways and cellular response. Here, the most recent studies on the in-vitro cytotoxicity of NPs, nanowires and nanorods for biomedical applications are reviewed and divided into two parts. The first part considers nonmagnetic metallic and magnetic nanostructures, while, the second part covers carbon structures and semiconductors. The factors influencing the toxicity of these nanostructures are elaborated to help elucidate the effects of these nanomaterials on cells, which is a prerequisite for their safe clinical use.",signatures:"Jose E. Perez, Nouf Alsharif, Aldo I. Martínez-Banderas, Basmah\nOthman, Jasmeen Merzaban, Timothy Ravasi and Jürgen Kosel",downloadPdfUrl:"/chapter/pdf-download/62231",previewPdfUrl:"/chapter/pdf-preview/62231",authors:[{id:"141848",title:"Prof.",name:"Jurgen",surname:"Kosel",slug:"jurgen-kosel",fullName:"Jurgen Kosel"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"8073",title:"Chromosomal Abnormalities",subtitle:null,isOpenForSubmission:!1,hash:"6a9d3c58434edf5e65f9849a6858edfe",slug:"chromosomal-abnormalities",bookSignature:"Tülay Aşkın Çelik and Subrata Dey",coverURL:"https://cdn.intechopen.com/books/images_new/8073.jpg",editedByType:"Edited by",editors:[{id:"74041",title:"Dr.",name:"Tulay",surname:"Askin Celik",slug:"tulay-askin-celik",fullName:"Tulay Askin 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Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],ofsBooks:[]},correction:{item:{id:"81439",slug:"corrigendum-to-the-development-biology-authentic-learning-of-mahasarakham-university-demonstration-s",title:"Corrigendum to: The Development Biology Authentic Learning of Mahasarakham University Demonstration School (Secondary), Thailand",doi:null,correctionPDFUrl:"https://cdn.intechopen.com/pdfs/81439.pdf\r\n",downloadPdfUrl:"/chapter/pdf-download/81439",previewPdfUrl:"/chapter/pdf-preview/81439",totalDownloads:null,totalCrossrefCites:null,bibtexUrl:"/chapter/bibtex/81439",risUrl:"/chapter/ris/81439",chapter:{id:"78086",slug:"the-development-biology-authentic-learning-of-mahasarakham-university-demonstration-school-secondary",signatures:"Wutthisak Bunnaen",dateSubmitted:"April 27th 2021",dateReviewed:"June 8th 2021",datePrePublished:"August 13th 2021",datePublished:"February 9th 2022",book:{id:"9558",title:"Active Learning",subtitle:"Theory and Practice",fullTitle:"Active Learning - Theory and Practice",slug:"active-learning-theory-and-practice",publishedDate:"February 9th 2022",bookSignature:"Olena Lutsenko and Gregory Lutsenko",coverURL:"https://cdn.intechopen.com/books/images_new/9558.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"225667",title:"Mrs.",name:"Olena",middleName:null,surname:"Lutsenko",slug:"olena-lutsenko",fullName:"Olena Lutsenko"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"342787",title:"Dr.",name:"Wutthisak",middleName:null,surname:"Bunnaen",fullName:"Wutthisak Bunnaen",slug:"wutthisak-bunnaen",email:"wutthisakcomplete@gmail.com",position:null,institution:null}]}},chapter:{id:"78086",slug:"the-development-biology-authentic-learning-of-mahasarakham-university-demonstration-school-secondary",signatures:"Wutthisak Bunnaen",dateSubmitted:"April 27th 2021",dateReviewed:"June 8th 2021",datePrePublished:"August 13th 2021",datePublished:"February 9th 2022",book:{id:"9558",title:"Active Learning",subtitle:"Theory and Practice",fullTitle:"Active Learning - Theory and Practice",slug:"active-learning-theory-and-practice",publishedDate:"February 9th 2022",bookSignature:"Olena Lutsenko and Gregory Lutsenko",coverURL:"https://cdn.intechopen.com/books/images_new/9558.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"225667",title:"Mrs.",name:"Olena",middleName:null,surname:"Lutsenko",slug:"olena-lutsenko",fullName:"Olena Lutsenko"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"342787",title:"Dr.",name:"Wutthisak",middleName:null,surname:"Bunnaen",fullName:"Wutthisak Bunnaen",slug:"wutthisak-bunnaen",email:"wutthisakcomplete@gmail.com",position:null,institution:null}]},book:{id:"9558",title:"Active Learning",subtitle:"Theory and Practice",fullTitle:"Active Learning - Theory and Practice",slug:"active-learning-theory-and-practice",publishedDate:"February 9th 2022",bookSignature:"Olena Lutsenko and Gregory Lutsenko",coverURL:"https://cdn.intechopen.com/books/images_new/9558.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"225667",title:"Mrs.",name:"Olena",middleName:null,surname:"Lutsenko",slug:"olena-lutsenko",fullName:"Olena Lutsenko"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}}},ofsBook:{item:{type:"book",id:"9823",leadTitle:null,title:"Umbilical Cord Blood and Umbilical Cord Tissue - New Advances",subtitle:null,reviewType:"peer-reviewed",abstract:"
\r\n\tUmbilical cord blood (UCB) and umbilical cord tissue (UCT) have been worldwide stored cryopreserved in private and public cord blood and tissue banks in order to obtain hematopoietic (HSCs) and mesenchymal stem cells (MSCs) to be used in several clinical applications, most of them still under study in pre-clinical and clinical trials or even in in vitro testing. Deep knowledge of the regulatory issues is crucial for the implementation and development of umbilical cord blood and umbilical cord tissue banks where the quality of the samples is of the most importance. The UCT and UCB have been used in several clinical trials concerning a wide range of pathologies and diseases that can be daily consulted.
\r\n\r\n\tFurthermore, Advanced Therapy Medicinal Products (ATMPs) derived from UCB and UCT, from clinical translation, will bring exciting studies and new data for stem cells therapies, gene therapy, and gene editing, and immunotherapy promoting the Regenerative Medicine. UCB and UCT will probably become important in Regenerative Medicine therapies, presenting unique challenges related to product testing, scientific protocols, product quality and specifications, performance characteristics, and compliance criteria. This field of knowledge implies a multidisciplinary team where pre-clinical trials using appropriate animal models can also allow the application of cell-based therapies in Veterinary Medicine working in a One Health approach.
",isbn:null,printIsbn:"979-953-307-X-X",pdfIsbn:null,doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!1,isSalesforceBook:!1,isNomenclature:!1,hash:"f27ca50afb10a506c369451107433e90",bookSignature:"Prof. Ana Colette Maurício",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/9823.jpg",keywords:"Umbilical Cord Blood, Umbilical Cord Tissue, Cryopreservation, GMP, ATMPs, Cell-based Therapy Commecialization, Gene Editing, Gene Therapy, Regenerative Medicine, Tissue Engineering, Stem cells, Biomaterials",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:0,numberOfDimensionsCitations:0,numberOfTotalCitations:0,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"October 2nd 2019",dateEndSecondStepPublish:"March 11th 2020",dateEndThirdStepPublish:"May 10th 2020",dateEndFourthStepPublish:"July 29th 2020",dateEndFifthStepPublish:"September 27th 2020",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"2 years",secondStepPassed:!0,areRegistrationsClosed:!0,currentStepOfPublishingProcess:5,editedByType:null,kuFlag:!1,biosketch:null,coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"56285",title:"Prof.",name:"Ana Colette",middleName:null,surname:"Maurício",slug:"ana-colette-mauricio",fullName:"Ana Colette Maurício",profilePictureURL:"https://mts.intechopen.com/storage/users/56285/images/system/56285.jpeg",biography:"Ana Colette Pereira de Castro Osório Maurício has a degree on Veterinary Medicine since 1995, a PhD on Veterinary Sciences since 1999 from Faculdade de Medicina Veterinária (FMV) - Universidade de Lisboa (ULisboa) and Habilitation in Veterinary Sciences (ICBAS-UP) since 2011. The PhD experimental work was developed at Instituto Gulbenkian Ciência (IGC) in Oeiras, Portugal, at Freiburg Medicine Faculty in Germany and at Faculdade de Ciências e Tecnologia (FCT) from Universidade Nova de Lisboa (UNL). At the present, she is an Associated Professor with Habilitation, from the Veterinary Clinics Department of Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), she is the vice-President of the Pedagogic Council of ICBAS – UP. She is a Member of the Scientific Council and Member of the Representatives Council of ICBAS-UP. She is the Director of the Veterinary Sciences Doctoral Program at ICBAS – UP. She is the Scientific Coordinator of Regenerative Medicine and Experimental Surgery sub-unit from Centro de Estudos de Ciência Animal (CECA) of Instituto Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA). For the past 12 years she coordinates a multidisciplinary research group of Experimental Surgery and Regenerative Medicine, working with several biomaterials and cellular therapies. She started working with embryonic stem cells obtained by somatic nuclear transfer for therapeutic use; with Ian Wilmut´s group (Dolly’s cloned sheep). Several relevant publications had been produced and conducted to a PhD thesis that she co-supervised together with Ian Wilmut and to the first Portuguese cloned animal (R Ribas, B Oback, W Ritchie, T Chebotareva, J Taylor, AC Maurício, M Sousa, I Wilmut, 2006. Cloning and Stem Cells 8(1): 10; R Ribas, J Taylor, C McCorquodale, AC Maurício, M Sousa, I Wilmut, 2006. Biology of Reproduction 74: 307; R Ribas, B Oback, W Ritchie, T Chebotareva, T Ferrier, C Clarke, J Taylor, E Gallagher, AC Maurício, M Sousa, I Wilmut, 2005. Cloning and Stem Cells 7(2): 126). But ethical issues related to the collection and manipulation of human embryonic stem cells, even for therapeutic use is very controversial and understandable. So, more recently the potential of fetal stem cells derived from extra-embryonic tissues has been deeply investigated by her research group. Therefore, a continued effort to identify and characterize novel stem cell populations appears critical for widespread clinical success. This effort implies in vitro studies, experimental surgery and in vivo testing, before the clinical trials and the compassive treatment in such clinical cases where the traditional and standard treatments failed. Her research groups works exactly in this direction, so she created a multidisciplinary team, including Veterinaries, Engineers, Medical Doctors that through Experimental Surgery have a crucial role in the development of biomaterials and cellular therapies, allowing a close share of knowledge between biomaterials design, development of cellular systems, and surgeons needs when related to specific clinical cases. This group has several recent relevant publications in the research areas of nerve, bone, musculoskeletal and vascular tissue regeneration. In her laboratory have been working several PhD and Post-Doctoral students from various countries who have acquired a high level of competence in the study of tissue regeneration. She is the supervisor of several PhD, Post-Doctoral and Master students (16 PhD thesis already concluded with success and 14 PhD thesis on going), she is the co-author of a large number of scientific articles published in Indexed Journals (she publishes as Maurício AC) and of several scientific book chapters. She was the principal researcher of several national and international scientific projects. Editor of three international scientific books, inventor of three international patents.",institutionString:"University of Porto",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"10",totalChapterViews:"0",totalEditedBooks:"2",institution:{name:"University of Porto",institutionURL:null,country:{name:"Portugal"}}}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"16",title:"Medicine",slug:"medicine"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"280415",firstName:"Josip",lastName:"Knapic",middleName:null,title:"Mr.",imageUrl:"https://mts.intechopen.com/storage/users/280415/images/8050_n.jpg",email:"josip@intechopen.com",biography:"As an Author Service Manager my responsibilities include monitoring and facilitating all publishing activities for authors and editors. From chapter submission and review, to approval and revision, copy-editing and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. I maintain constant and effective communication with authors, editors and reviewers, which allows for a level of personal support that enables contributors to fully commit and concentrate on the chapters they are writing, editing, or reviewing. I assist authors in the preparation of their full chapter submissions and track important deadlines and ensure they are met. I help to coordinate internal processes such as linguistic review, and monitor the technical aspects of the process. As an ASM I am also involved in the acquisition of editors. Whether that be identifying an exceptional author and proposing an editorship collaboration, or contacting researchers who would like the opportunity to work with IntechOpen, I establish and help manage author and editor acquisition and contact."}},relatedBooks:[{type:"book",id:"5369",title:"Umbilical Cord Blood Banking for Clinical Application and Regenerative Medicine",subtitle:null,isOpenForSubmission:!1,hash:"711421bf3bdb0e540fc84267b82b1995",slug:"umbilical-cord-blood-banking-for-clinical-application-and-regenerative-medicine",bookSignature:"Ana Colette Mauricio",coverURL:"https://cdn.intechopen.com/books/images_new/5369.jpg",editedByType:"Edited by",editors:[{id:"56285",title:"Prof.",name:"Ana Colette",surname:"Maurício",slug:"ana-colette-mauricio",fullName:"Ana Colette Maurício"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"5852",title:"Peripheral Nerve Regeneration",subtitle:"From Surgery to New Therapeutic Approaches Including Biomaterials and Cell-Based Therapies Development",isOpenForSubmission:!1,hash:"d1cd2e797f008dcee9dd0c1010145eb8",slug:"peripheral-nerve-regeneration-from-surgery-to-new-therapeutic-approaches-including-biomaterials-and-cell-based-therapies-development",bookSignature:"Ana Colette Mauricio",coverURL:"https://cdn.intechopen.com/books/images_new/5852.jpg",editedByType:"Edited by",editors:[{id:"56285",title:"Prof.",name:"Ana Colette",surname:"Maurício",slug:"ana-colette-mauricio",fullName:"Ana Colette Maurício"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6550",title:"Cohort Studies in Health Sciences",subtitle:null,isOpenForSubmission:!1,hash:"01df5aba4fff1a84b37a2fdafa809660",slug:"cohort-studies-in-health-sciences",bookSignature:"R. 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Conventional way of quality initiatives such as ISO series, quality assurance, statistical quality control, zero defects, and total quality management has been the key initiatives for many years. The objective is to overcome challenges and survive in global competitive market [1]. The ability of business to adapt to new customer requirements in a globalized market is of vital importance for long-term success.
\nLiterature suggests that quality management practices are critical for small and medium-sized enterprises (SMEs) to gain a competitive advantage. Intriguingly, the systematic application of quality management practices in SMEs, despite the wide-ranging effort that has been invested and the long-term benefits that may be achieved, remains mostly uncommon [2]. Some possible causes which may account for the low usage of quality initiatives in SMEs have been publicized in previous studies. According to quality management literature [3, 4], the reasons vary from difficulty in differentiating between different initiatives such as International Organization for Standardization (ISO), Total Quality Management (TQM), Six Sigma, and Lean to lack of clarity about the advantages of one initiative over other. SMEs also feel that ISO as an initiative is sufficient enough to meet their business needs. However, most of the studies have focused on factors related to cultural, organizational, and project differences [5, 6]. A few have examined the influence of those factors pertinent to the tool itself such as worth and user friendliness. The comparative importance of these factors on the application of quality management practices has never been discussed. In addition, these studies tend to examine only the diffusion of quality management tools and techniques in industrial countries such as the US, Britain, and Japan [7], with no prior study in developing economies such as India.
\nAccording to Small and Medium Business Development Chamber of India:
\n“SMEs play a vital role in the growth of Indian economy by contributing 45% of the industrial output, 40% of exports, 42 million in employment, create one million jobs every year and produces more than 8000 quality products for the Indian and international markets.”
\nFurther Indian industries such as Manufacturing, Precision Engineering, Food Processing, Pharmaceuticals, Textile and Garments, Retail, IT, Agro, and Service sectors are growing rapidly, there are increasing opportunities for SMEs to enhance their business activities. This phenomenon is not unique to India but has been observed worldwide [8].
\nLiterature also suggests that SMEs should view quality improvement as a journey and not as a destination. The SMEs should learn from large organizations and continuously strive for performance excellence by implementing initiatives such as Lean and Six Sigma [7]. Irrespective of growing importance of SMEs in India, there are limited studies (such as [9, 10]) about the extent of quality initiatives implementation in SMEs. To address this issue, the chapter analyses the context of Indian SMEs and provides empirical evidences related to quality initiatives. The main aim of this chapter is to empirically investigate the current state and extent of quality initiatives implementation in Indian SMEs.
\nThe research contributes significantly to academia and practice. First, the chapter explores through literature the current state of quality initiatives implementation in SMEs throughout the world. Second, it provides empirical evidence of quality practices in Indian SMEs. As the quality practices in Indian SMEs is still at nascent stages (except in automobile or electronic sectors, [9]), this type of study could enhance the thinking of government policy makers and top management to adopt quality initiatives for better management style and preparing SMEs for both local and global competition [1].
\nThe application of QM practices in SMEs has attracted significant interest among practitioners and researchers in quality and process improvement [11, 12]. Since late 1990s, Lean and Six Sigma have been the most widely accepted methodologies for operations improvement and quality management [11, 12, 13, 14]. Recently, several practitioners have reported appreciable cost savings, profit maximization, and waste reduction [15, 16]. Lean is a collection of tools aimed at reducing cost while improving the operational speed of businesses processes by eliminating seven types of wastes. This is achieved through company-wide involvement, applying tools such as continuous flow, Kaizen, root cause analysis, value stream mapping, Just-in-Time (JIT), Total Productive Maintenance (TPM), Kanban, and bottleneck analysis [17, 18]. On the contrary, Six Sigma is centered on the application of a well-structured data-driven problem-solving methodology defined by the acronym DMAIC, that is, Define, Measure, Analyze, Improve, and Control. The methodology has a strong focus on meeting customer needs and reducing variation in the process performance [15].
\nUnderstanding the critical success factors (CSFs) and the barriers to QM implementation is crucial for significant and sustainable benefits [8]. Among others, customer focus, particularly the voice of the customer, is the most crucial QM principle [11]. While conducting a survey of manufacturing SMEs in the UK, [4] found that, among 11 critical success factors (CSFs), the most important factors for the successful implementation of Six Sigma are management involvement and participation, linking Six Sigma to customers and linking Six Sigma to the business strategy of the organization.
\nRelated empirical studies exist in the literature. Kumar [7] conducted a comparative study of Six Sigma implementation in the UK manufacturing SMEs and found that lack of knowledge and limited resource availability are the main reasons for not implementing quality initiatives in the companies. Recent investigations by [19] show that TQM practices have significant positive effects on competitive advantage and organizational performance. On the other hand, [20] investigated the effect of QM practices on the performance of banks in Jordan. Nulty [21] studied the impact of Lean Six Sigma tools on the profitability of SMEs in Nigeria.
\nGiven the widespread awareness of the importance of SMEs in the world economy, it is necessary to reinvestigate the level of implementation of known quality initiatives in SMEs. These practices have been known to assist SMEs in achieving incremental process and product innovation [22, 23]. A significant number of studies have been done in the developed world, including the UK [5, 22], Australia [7, 11, 24, 25], and other industrialized nations such as USA and Japan [7, 11].
\nPast research tends to focus on investigating only the diffusion of quality initiatives in industrial countries. Very limited studies have paid attention on developing economies, for instance, from the African and Asian regions [21, 26]. Prajogo [26] studied the critical success factors of QM practices among SMEs in the food processing industry in Malaysia. Shafer and Moeller [27] investigated quality management and the performance of SMEs in the Indian industries. Majumdar and Manohar [2] carried a survey of quality initiatives among manufacturing SMEs in Pakistan. Nulty [21] investigated the effects of Lean Six Sigma methodologies on the profitability of SMEs in the Nigerian context. Preliminary studies on the application of quality management tools in the Namibian context have shown that most SMEs are not aware of high-level quality initiatives such as Six Sigma and Lean [28]. However, a considerable number of SMEs in the country have realized the need for responding to the voice of the customer.
\nAddressing this gap, this study attempts to investigate the adoption of quality management tools and the barriers behind their implementation in Indian SMEs. It is hoped that the study will provide a number of insights and strategies for effective implementation of quality initiatives for SMEs, enabling the SMEs to realize benefits from the use of the quality initiatives (basic and advanced). Further, the study may help to reveal the interesting common and contrasting characteristics between SMEs and to draw important lessons for incremental process innovation and SME performance.
\nAn exploratory survey of small and medium enterprises in and around Tiruchirappalli (Southern part of India) was conducted. The purpose was to understand the extent of implementation of quality management practices in these enterprises. According to [29], this kind of exploratory survey provides preliminary evidence of association among concepts as well as exploring valid boundary of a theory. According to [30], survey research is about collecting data from a population or some samples drawn from a population with a focus “to assess the relative incidence, distribution and interrelationships of naturally occurring phenomena” [7]. The researchers in quality management area focus more on data collection through survey to validate hypotheses and research questions [7].
\nThe questionnaire had five parts. The first part of the questionnaire was intended to get some general information of the respondent organization, such as
size and type of organization (local, multinational, or joint venture),
whether they have quality department,
there is a proper quality system in place,
whether they have implemented quality initiatives.
It is also designed as a filter to segregate the data based on organizations that have or have not implemented quality initiatives.
\nThe second part of the questionnaire attempted to identify the critical success factors that are important while implementing quality initiatives in organizations. The third part consisted of two questions. First question was directed at identifying business performance indicators that are to be improved through quality initiatives. Second question explored the tools and techniques used in implementation of quality initiatives. The fourth part was for those SMEs that have not implemented quality initiatives. There was one question in this part to explore about the reasons behind not implementing quality management practices. The last part was designed to obtain background information on respondents including their name, job title, company, mailing address, phone/fax number, and e-mail.
\nOne of the main concerns while designing questionnaire is to have a proper response format. This helps in safeguarding against alteration in the type and wording of the question, as well as the type of analysis researcher wants to perform [31]. We used a closed-ended questionnaire format to collect quantifiable data, in order to perform statistical analysis. Further, this kind of format makes it easy to complete, facilitates faster data entry, and thus enabling better data analysis and summarizing the findings [32]. The questionnaire included questions on critical success factors, business performance indicators, and quality initiatives grounded in literature. A 5-point Likert-type scale was used to measure critical success factors and quality initiatives (Critical success factors: 1 = no influence, 5 = very high influence; Quality initiatives: 1 = never, 5 = always). Mutingi [33] suggests the use of Likert-type scale as it provides precise measure in comparison to a yes/no or true/false items and is also faster and easier to complete. The rating type scale facilitates researcher’s understanding about critical issues or factors as the format allows respondents to indicate relative importance of choices [31].
\nThe dependent variables for this research are:
Critical success factors (CSFs)
The literature on quality management discusses about CSFs important for implementing quality initiatives in SMEs. These CSFs are mentioned without any rigorous proof [34, 35]. The CSFs specific to SMEs are mentioned in a few literatures, with support coming from surveys [7, 11]. In our questionnaire design, we follow these previous studies and included CSFs that are important from quality management point of view.
Business performance indicators
The business performance indicators are not much explored in the literature, and there is no specific study exploring it specifically for SMEs. In our survey, we included business performance indicators from previous limited studies [7] and explored further. The survey helped us in preparing a list of business performance indicators related to SMEs, which we want to explore further.
Tools and techniques
There is much literature mentioning tools and techniques used in different organizational process improvement initiatives. Literature focusing on tools and techniques specific to quality initiatives such as Six Sigma in SMEs is limited barring a few studies [31]. Through the survey, we explored the importance placed by SMEs on tools and techniques.
Reasons
The literature focused on SMEs, in describing the difficulties or reasons behind limited use of quality initiatives. The studies though lack academic rigor, they are mainly theoretical in nature. The survey questionnaire included differences and reasons based on previous studies.
\nThe independent variables are:
Demographic information
Type of organization—multinational, local, and joint venture
Type of SME
Size of SME
Employee size—size or range of full-time employees in the organization
Whether the organization has implemented quality management practices or not
Yes
How long has your company been using the quality initiative
Percentage of your business process that you are applying quality initiatives
Your skills in quality initiatives
No
Reasons behind not implementing quality initiatives
The survey was conducted in and around Tiruchirappalli or Trichy based in South India. The city is around 320 km south of Chennai. It is famous for its temples, educational institutes, and public sector units such as Bharat Heavy Electricals Limited (BHEL) and Ordinance Factory. BHEL came into existence in 1960s and has initiated development of industrial estates. This lead to rapid growth in SMEs that increased from 36 to more than 300 in a short span of time.
\nIn recent times, introduction of new products such as windmills, rice husk boilers, and improved government funding in power sector; turnover for SMEs has grown in rapid proportions. The total reported turnover of SMEs was around US$ 500 million, out of which medium scale enterprises contributed around US$ 375 million, while the balance is contributed by small-scale enterprises [36].
\nThe enterprises are also the major employers, employing around 20,000 workers. Though the major buyer is BHEL that accounts for almost 70% of the total production, recent emergence of new clients such as GE, Suzlon, Caterpillar, etc. has increased the competitiveness and array of products among SMEs [36].
\nThere are mostly fabrication enterprises, followed by machine shops, and lastly small units. These small units are engaged in the activities such as shot blasting, galvanizing, drilling, etc., while some of them are manufacturers of electrodes, grinding wheels, paints, etc. In total, there are 250 fabricators, 75 machine shops, and less than 75 small units [36].
\nGiven our proximity to such a large cluster of SMEs, the survey was directed toward this population. This helped us sometime in visiting the organizations, in case they were nonresponsive to our surveys.
\nFor the purpose of this survey, the sample units to be surveyed were divided into two categories on the basis of their turnover (refer Table 1). They are:
\nS. No. | \nDescription | \nAnnual turnover (in Rs million) | \nTotal number of units | \nSample number of units | \n
---|---|---|---|---|
1 | \nMedium scale units | \n50 or higher | \n20 | \n16 | \n
2 | \nSmall scale units | \n5–50 | \n250 | \n36 | \n
Incomplete (not usable) | \n13 | \n|||
Total sample | \n65 | \n
Overview of the sample.
A total of 16 medium-scale units and 36 out of 250 small-scale units responded to our survey. Responses from 13 units (medium- and small-scale) were incomplete and were excluded, thus leaving 52 usable questionnaires and a response rate of 19.52%.
\nThe survey was conducted by mailing the questionnaire along with a cover letter on the institute letterhead to all the SMEs in the targeted population. The purpose of the letter is to make the enterprises familiarize with our research by clearly stating the objectives and benefits of this exercise. Following [7], we designed the survey in a manner to improve the response rate. Therefore, a follow-up letter reminding to send the responses was mailed to those who have not replied. This is done simultaneously with multiple visits to those enterprises by the researchers and research assistants that are in proximity to the institute. Follow-up letter and visits helped in increased response rate (around 30%) from small units. The respondents were offered no incentives except a summary of research findings if they have shown interest by checking the box provided in the questionnaire.
\nThe questionnaire was posted to 270 SMEs who were in the mailing list (refer Table 2). The mailing list was based on the information provided by the District Industries Center (DIC) of Trichy. DICs keep database of Micro, Small, and Medium Enterprises and formulate schemes for the development of the sector. A total of 52 were completed, 30 undelivered due to incorrect address, and 10 enterprises declined to participate. Based on the completed responses received, we observed that around 60% of the organizations have some form of quality initiatives, while 25% have not implemented any quality initiatives; remaining enterprises still need to be educated about different quality initiatives. As we focus on both types of enterprises which have or have not implemented quality initiatives, the usable responses for our study are 52. This is similar to the previous studies conducted elsewhere in the world (for e.g. [8]).
\nStatus | \nNumber | \nResponse rate (out of all organizations) (%) | \n
---|---|---|
Total sent | \n270 | \n\n |
Undelivered | \n30 | \n11 | \n
Declined invitation | \n10 | \n4 | \n
Returned (usable) | \n52 | \n19.52 | \n
Overview of the sample.
There are various reasons for low response rate in our study. One is no clear indication of the SMEs that have implemented quality initiatives. The second limitation is inaccuracy in the list in terms of name and position of the respondents, or targeted organizations’ mailing address is not properly updated. The list also failed to highlight the enterprises that have closed down. Given the limitations and reviewing the literature on similar studies, we found the response rate for our study acceptable to make meaningful conclusions [7, 11].
\nConcerns about bias emerging from low response rate are frequently expressed in survey research literature. A study of the literature about survey response rate in quality management shows that the variation is from 11.5 to 25.2% (refer Table 3).
\nSl. No. | \nAuthors | \nManagement system | \nCountry | \nSample targeted | \nOrganizations responded | \nResponse (%) | \n
---|---|---|---|---|---|---|
1 | \nBeaumont and Sohal [39] | \nISO 9001 | \nAustralia | \n252 | \n59 | \n23 | \n
2 | \nAntony [40] | \nSix Sigma | \nUK | \n200 | \n28 | \n14 | \n
3 | \nBhuiyan and Alam [41] | \nISO 9001 | \nCanada | \n138 | \n30 | \n22 | \n
4 | \nChan [42] | \nISO 9001 | \nHong Kong | \n330 | \n83 | \n25.2 | \n
5 | \nKumar and Antony [43] | \nIMS | \nUK | \n500 | \n64 | \n12.8 | \n
6 | \nBernardo et al., [44] | \nIMS | \nSpain | \n1615 | \n435 | \n27 | \n
7 | \nKumar et al., [45] | \nIMS | \nUK and Australia | \n500 and 800 | \n64 and 92 | \n12.4 and 11.5 | \n
Questionnaire surveys and response rates (adapted from Khanna et al. [46]).
Thus there is a growing body of knowledge which seems to suggest that low response rate does not necessarily means that there is a high nonresponse bias (please see [37, 38]).
\n“Assembly of [survey-related] methodological studies whose designs permit estimation of nonresponse bias shows that empirically there is no simple relationship between nonresponse rates and nonresponse biases. That is, research results comport with the assertion that covariance between survey variables and response propensities are highly variable across items within a survey, survey conditions, and populations. Hence, there is little empirical support for the notion that low response rate surveys de facto produce estimates with high nonresponse bias (pg. 670)”. (As observed from [36])
\nAnother factor which connects with low response rates is representativeness. But studies such as those in Table 3 and also by [7] suggest that as long as the samples include different types of companies in the region, representativeness of the respondent balance the bias due to low response rate. Accordingly, this study is able to gather information from SMEs having diverse businesses and thus is representative of the population within the given region.
\nIn summary, the current literature suggests shift in view about nonresponse bias and representativeness arising from low response rates in surveys. New research seems to suggest that the influence of low response rate on bias and representativeness is less straightforward in contrary to the assumption among researchers [36].
\nThe types of SMEs which participated in the survey include manufacturers of boiler parts and boiler components, cement plant equipment, steel plant equipment, fuel firing equipment such as burners, valve, and valve manufacturing, camshafts manufacturing, automotive parts manufacturers, and others. Others involve furniture manufacturers, waste and wastewater treatment product manufacturers, and some service providers such as computer-aided design services to product development companies. It is observed that the core manufacturing companies such as boiler components, cement and steel plant equipment, and automotive components manufacturers mainly use quality initiatives, and they have turnover of more than 50 million. On the other hand, smaller enterprises such as furniture manufacturers or wastewater treatment plant component manufacturers do not use any quality initiatives.
\nAs the present study is to explore about quality initiatives implementation in small and medium enterprises, we did not explore further behind to understand why small enterprises are not implementing quality initiatives. But we like to understand the reasons further through qualitative study of these organizations and develop a framework to spread implementation of quality initiatives implementation in Indian SMEs.
\nSuccess factors as concept was introduced by [38] and later popularized by [47]. Prajogo [47], extending ideas from [38], defines CSFs as “the limited number of areas in which results, if they are satisfactory, will ensure successful competitive performance for the organization.”
\nThe above definition proposes to “identify an ideal match between environmental conditions and business characteristics for a particular company” [48].
\nThe CSFs (along with their average scores) identified from this study are shown in Table 4.
\nCSFs | \nAverage score | \n
---|---|
Projects with small team | \n4.25 | \n
Management involvement and commitment | \n4.00 | \n
Communication | \n3.94 | \n
Cultural change | \n3.94 | \n
Team members with great motivation | \n3.89 | \n
Project management skills | \n3.81 | \n
Frequent feedback and measurement | \n3.75 | \n
Organizational infrastructure and culture | \n3.69 | \n
Projects with multiple independent team | \n3.64 | \n
Right amount of documentation | \n3.61 | \n
Critical success factors.
The study included 18 CSFs after going through the literature. These CSFs were identified from studies that focused on quality initiatives in SMEs in general or from studies on specific practices such as Lean and Six Sigma implemented in the enterprises. All the CSFs have been rated from medium to high influence. CSFs such as Projects with small teams and Management involvement and commitment were rated as high influence. This is understandable given SMEs have less number of employees, and this prevents forming large teams for quality initiatives. Also for any quality management practices to be initiated and implemented need continuous support from top management, which in most cases also involves the owner of the enterprise [7].
\nOur study further identified other CSFs having near high influence include communication and culture change. These are very important given the context of our study. As BHEL (Bharat Heavy Electricals Limited) is the major customer for most of the surveyed SMEs, it requires major shift in the culture of these SMEs to focus on quality initiatives and not being complacent to satisfy a single customer.
\nLiterature suggests business performance indicators as customer requirements translated by organizations from Voice of Customer (VoC) [34]. Prior studies have not much focused on business performance indicators from an SME perspective. We identified 10 business performance indicators relevant to SMEs and found from the responses that all of them fall into the category of moderate to high relevance. Business performance indicators such as proximity to consumer and warranty returns are rated to have high relevance (refer Table 5). This can again be understood based on the context. BHEL and Ordinance Factory (both Public Sector Units (PSUs)) being major customers for most of the SMEs make the above-mentioned business performance indicators very relevant. As discussed in earlier section, the PSUs contribute toward 70% sales of the products for the SMEs in and around Tiruchirappalli.
\nCTQs | \nAverage score | \n
---|---|
Proximity to customer | \n4.49 | \n
Warranty returns | \n4.29 | \n
Relationship management | \n4.18 | \n
Correct invoice | \n4.12 | \n
Percentage of customer order | \n4.11 | \n
New product development | \n4.06 | \n
Price satisfaction | \n4.03 | \n
Brand image | \n3.96 | \n
On-time delivery | \n3.35 | \n
Special order lead time | \n2.92 | \n
Business performance indicators.
This argument can further be strengthened as the other relevant business performance indicators highlighted by the respondents include special order lead time, on-time delivery, and price satisfaction all pointing toward the importance of PSUs as customers for these SMEs.
\nPrior studies on SMEs have focused on the application of various tools and techniques. Examples include studies carried out by [4, 8, 11]. Based on the literature, we have identified tools and techniques which are commonly used in SMEs for our study. The responses showed (refer Table 6) that most of these tools and techniques are used occasionally by the SMEs.
\nTools | \nAverage | \n
---|---|
Overall Equipment Effectiveness | \n4.00 | \n
Root Cause Analysis | \n4.00 | \n
Bottleneck Analysis | \n3.86 | \n
PDCA (Plan, Do, Check, Act) | \n3.79 | \n
Single Minute Exchange of Die (SMED) | \n3.79 | \n
Poka – Yoke (Error proofing) | \n3.43 | \n
Inspection | \n3.36 | \n
Value Stream Mapping | \n3.00 | \n
Frequency of usage of quality management practices by SMEs.
This can be attributed to availability of major customers for these SMEs, providing them steady order and thus keeping them in business. It also highlights the need for education to these SMEs about various quality initiatives along with tools and techniques that can be beneficial in improving their bottom line.
\nThe reasons for not implementing or widely using quality initiatives by the respondents are presented in Figure 1. There are two types of respondents in our study. One set of SMEs has some quality initiatives in place, remaining are still to decide on implementing the initiatives. High cost of training emerged as one of the major reasons the SMEs are shying away from quality initiatives. Complexity in learning, time consuming efforts, difficulty in identifying process parameters, and difficulty in data collection further restrict the usage of quality initiatives by the SMEs. High percentage of respondents also mentioned about limited or no knowledge about quality initiatives. This shows the need for better education and exposure on various quality initiatives to these SMEs by academic and practitioner community.
\nReasons for not implementing quality initiatives.
In this study, we focused on understanding the extent of quality initiatives implementation in Indian SMEs. First objective of the study was to observe the critical success factors (CSFs), which are important for the SMEs. The results show projects with small team as one of the important factors. This factor identified in our study is different from existing studies (e.g., [1, 7, 11]). Other factors such as management commitment, communication, and cultural change relate to the “softer side” (or “human side”) of the quality initiatives implementation [11, 25, 49].
\nBusiness performance indicators are important criteria that help the firm to win customer loyalty [7]. The objective here is to understand the importance given to customers by the SMEs in order to gain customer loyalty. Proximity to customer, warranty returns, and relationship management featured highly influential for the units to gain customer loyalty.
\nThird objective of our study was to identify tools and techniques usage by the SMEs while implementing different quality initiatives. The literature talks about TQM, Six Sigma, and Lean implementation in Australian and UK SMEs [1, 11]. We found most of the SMEs were ISO 9001:2015 [38] certified based on the ISO 9000 [50] principles. These SMEs uses techniques such as overall equipment effectiveness, root cause analysis, and PDCA cycle. High adoption of ISO 9001 by SMEs is in consensus with the literature [51, 52]. High adoption of ISO 9000 by SMEs is in consensus with the literature [51, 52]. Further findings of low adoption of Six Sigma and Lean are also in agreement with literature [11].
\nFinally, we wanted also to identify the reasons behind SMEs not adopting quality initiatives. High cost of training and unknown to us emerged as major barriers to quality initiatives implementation. SMEs not knowing about quality initiatives are an area which calls for attention. This shows there is limited knowledge about different initiatives irrespective of wide spread success associated with different quality initiatives. This calls for systematic education and training required in these organizations to spread the implementation of quality initiatives. Literature suggests lack of resources and lack of top management commitment as impeding factors in introducing quality initiatives in SMEs [4, 11, 16].
\nThe study showed the importance of team and management commitment as important success factors. Therefore, managers of Indian SMEs need to play an important role in promoting quality initiatives. As most of the SMEs are near to the customer in this study, the managers need to focus on warranty returns and building long-term relationship with the customer. Further, there is a need for managers to understand the importance of education and training of employees in different quality tools and techniques, problem-solving skills, data analysis, and statistical techniques [1]. According to [53], managers need to understand and satisfy the individual employee needs and also improve the quality of life for employees. This will help in successful adoption of quality initiatives. Also, government policy makers can coordinate with senior management in SMEs and focus on developing training programs to enhance employee skill and thus prepare the organizations to adopt quality initiatives such as TQM, Lean, and Six Sigma, which will enable them to compete locally and globally.
\nMethodological fit is a well-developed field in management research. Edmondson and McManus [54] in their paper have discussed extensively about methodological fit at different phases of study based on the existence of relevant theory. According to their research, they suggest at exploratory stage:
\n“The research questions are more open-ended than those used to further knowledge in mature areas of the literature. In studies where theory is nascent or immature, researchers do not know what issues may emerge from the data and so avoid hypothesizing specific relationships between variables” (p. 1162).
\nFurther, [32] suggests to use exploratory surveys as basis for developing concepts and methods for future descriptive and explanatory surveys. In short, as suggested by [55], the whole purpose of an exploratory survey is to elicit a wide variety of responses from individuals with varying viewpoints in a loosely structured manner as the basis for design of a more careful survey.
\nFinally, we like to state that limited sample size has restricted rigorous statistical analysis as well as generalizability. But this can be overcome by using multiple stage study as followed by [56] or progressing through different stages of theory as explained by [54]. Our study response rate is acceptable based on prior similar studies such as by [56] and [2], where the response rate was between 23 and 25%. As discussed by [57], low response rate is acceptable as long as the sample is representative of the population. In our study scenario, we feel the representativeness of our sample and thus we are able to identify the required variables of our study through exploration.
\nThe purpose of our study was to explore and understand the extent of quality initiatives usage in SMEs. The study was carried out in Tiruchirappalli, which has a cluster of SMEs catering to the two big PSUs in the region. The findings lead to insights related to quality initiatives in these SMEs. We were able to see the effect of nearness of major customers in the area, as evidenced from the high relevance of business performance indicators such as proximity to customers, relationship management, percentage of customer orders, etc.
\nTools and techniques usage by SMEs vary from seldom to occasionally, and it is a cause for concern. The reason can be inferred back to known customers for these SMEs. Further probing into the reasons of not applying quality initiatives reveals high cost of training and no knowledge of tools and techniques as main deterrent for the SMEs. This needs to be further explored through in-depth case studies with some of these SMEs.
\nFinally, the study is an attempt to understand and evaluate the quality management practices in SMEs from a specific geographic location. This lead to some differences in the preferences from existing studies which is both a strength and limitation of this chapter. The strength lies in bringing a different perspective from the present studies. The study is relevant for the SMEs because they have very limited focus. The findings from this study will help them in looking beyond and extending the customer base. By avoiding the problems highlighted in our study, the SMEs can learn to educate themselves in quality management practices.
\nAs mentioned earlier, sample distribution may be regarded as a limitation of the study, because all respondents were from a single geographic location, limiting the generalizability of the findings to SMEs of a specific region (Southern India). Low response rate is another limitation, which we plan to overcome by conducting multilevel multiple exploratory case studies with SMEs identified from the survey response. The case study research (with cross-case analyses) will help us understanding different quality management practices across SMEs [30]. We also plan to conduct a national level survey of SMEs. Future research can also focus on a global survey and understand the cultural effect on successful implementation of quality initiatives in SMEs.
\nHysterectomy is one of the most commonly performed surgeries in the United States. In fact, Merrill et al. reported a 45% lifetime risk of hysterectomy [1] with an overall rate of 5.4 per 1000 women per year. The majority of hysterectomies are performed for benign gynecologic conditions—that is, the presence of fibroids. Other indications include abnormal uterine bleeding, uterovaginal prolapse, and pelvic pain. Hysterectomy can be performed via multiple routes—abdominally, laparoscopically (including robotic approach), or vaginally. Vaginal and laparoscopic procedures are considered minimally invasive surgical approaches based on the ability to avoid a large abdominal incision. These routes of hysterectomy are associated with shortened hospitalization and postoperative recovery when compared to the abdominal approach. As a result, analysis of U.S. surgical data demonstrates evolving practice patterns with an increase in minimally invasive hysterectomies and a decrease in abdominal hysterectomies [2, 3].
The Centers for Disease Control and Prevention defines surgical site infection (SSI) as an infection that occurs after surgery near the surgical site within 30 days following surgery or 90 days where an implant is involved. They can range from superficial infections involving skin, or more serious infections involving tissues underneath the skin, organs, or implanted materials. As such, SSI is classified as superficial, deep, or organ/space. The CDC monitors SSI via the National Healthcare Safety Network with reported SSI rates of 1.7% and 0.9% after abdominal and vaginal hysterectomy respectively [4].
In a retrospective cohort study of 23,366 patients undergoing laparoscopic and abdominal hysterectomy between the years 2005 and 2011, 783 (3%) developed a surgical site infection. The majority of these were wound infections with approximately ¼ of cases being infections of the organ space which represents 0.7% of the entire cohort [5]. A more recent large cohort study examining patients between the years 2012 and 2015 demonstrated a 2% incidence of postoperative infection after hysterectomy [6]. When stratified between abdominal versus minimally invasive approaches, the incidence of SSI in the abdominal hysterectomy group exceeded 1%, while the incidences in the other groups were 0.2–0.3% [7, 8, 9].
It is well known that postoperative infections are associated with increased patient morbidity and mortality, and may result in additional costs, extended hospital stays, and prolonged antibiotic use. On average, patients who had an SSI following hysterectomy incur twice the cost of care of their counterparts who did not have an SSI. In a study examining the clinical and economic burden of surgical site infection following hysterectomy, the highest cost owing to SSI ($19,203; 95% CI 17,260–21,365) was for abdominal hysterectomy. In addition, those who had SSI had a mean length of stay (LOS) that was between three and fivefold the LOS of those who did not have an SSI irrespective of surgical approach [10]. SSI following index surgery is also associated with a significantly greater percentage of hospital readmissions. Surgical site infections after hysterectomy have serious implications on patient care and healthcare as a whole. This chapter will review the current literature on surgical site infection (SSI) after hysterectomy for benign indications and address various methods of prevention and treatment.
There are a variety of factors that influence the route of hysterectomy including informed patient preference, accessibility of the uterus, extent of extrauterine disease, size and shape of the vagina and uterus, concurrent procedures, available hospital technology and support, the nature of the case
Evidence supports that the vaginal approach is associated with better outcomes when compared with other approaches to hysterectomy. A Cochrane review analyzing 47 randomized control trials with a total of 5,102 women determined that vaginal hysterectomy resulted in quicker return to normal activity when compared to abdominal hysterectomy. There was no difference in satisfaction, quality of life, and surgical complications. Similarly, laparoscopic hysterectomy also resulted in more rapid recovery, fewer febrile episodes, and lower incidence of SSI when compared to the abdominal approach [12]. In this systematic review, there were no advantages of laparoscopic over vaginal hysterectomy. In addition, the laparoscopic approach was associated with longer operating times and increased rates of urinary tract injuries [13]. As a result, a vaginal approach continues to be the preferred route of hysterectomy.
When it is not feasible to perform a vaginal hysterectomy, a surgeon must choose between a laparoscopic or an open abdominal approach. A Cochrane review demonstrated faster return to normal activity, shorter hospital stay, fewer infections, and improved quality of life in patients undergoing laparoscopic versus abdominal hysterectomy. However, operating times were longer with higher rates of lower urinary tract (bladder and ureter) injuries in the laparoscopy group [13].
When stratified by the type of hysterectomy
When stratified into various forms of laparoscopic hysterectomy including robotic hysterectomy, laparoscopic-assisted vaginal hysterectomy, and single-port hysterectomy, the authors concluded that more research was needed to determine if there is in fact, a benefit over conventional laparoscopic approaches. The largest study available on single port laparoscopy in gynecology was a retrospective study from Cleveland Clinic reviewing a total of 908 cases. The authors concluded that single port access was safe and feasible in gynecologic surgery inclusive of both malignant and premalignant conditions with a low rate of adverse outcomes. Perhaps the most prevalent adverse outcome is an increased risk of incisional hernia with a rate of 5.5% [15, 16]. Well-designed studies that compare outcomes of alternative hysterectomy routes (robotic, laparoscopic assisted vaginal, and single-port) are needed to determine if patients may benefit from these other approaches.
Although minimally invasive routes to hysterectomy remain the preferred approach, open abdominal hysterectomy is still an important surgical option for some patients. Open abdominal hysterectomy may become necessary in a variety of clinical scenarios including failure of to maintain a minimally invasive approach.
Preoperative medical optimization is critically important in risk reduction for SSI prior to hysterectomy. Eliminating particular risk factors for SSI contributes vastly to perioperative care. This includes taking an in-depth medical history, performing a comprehensive physical exam, and addressing the patient’s medical comorbidities. Patients should be counseled on modifiable and nonmodifiable risk factors such as smoking status, diabetes stabilization, anatomic anomalies, renal comorbidities, hydrosalpinx, endometrioma, prior laparotomy, and untreated pelvic inflammatory disease (PID) or bacterial vaginosis [17, 18, 19, 20]. Optimal diabetes control is critical in preventing postoperative SSI with both spot glucose levels ≤200 mg/dl and hemoglobin A1C levels below 8.5–9.0% [21, 22].
Preoperative screening for genital tract infections is generally not necessary; however, certain types of infections are clinically important prior to hysterectomy. It has been well established that bacterial vaginosis (BV) is associated with an increased risk of postoperative cuff cellulitis and subsequent pelvic abscess formation after hysterectomy [23]. Treatment of BV prior to scheduled hysterectomy will decrease this risk.
Practicing safe, high-quality, evidence-based operating room care begins first with accurate identification of the patient, surgical site, and procedure.
In an AAGL white paper, “Enhanced Recovery and Surgical Optimization Protocol for Minimally Invasive Gynecologic Surgery”, infection prophylaxis can be achieved via the implementation of SSI prevention bundles [24]. Quality or safety bundles provide a framework for the implementation of evidence-based practices. They have been validated across multiple disciplines to actually decrease SSI [25, 26, 27, 28]. The ACOG Council on Patient Safety in Women’s Health Care has published a consensus bundle on prevention of SSI prior to gynecologic surgery. This provides a framework for hospitals to develop, implement, and practice evidence-based prevention of SSIs [29].
An example of a hysterectomy bundle is as follows:
The degree of contamination at the time of surgery is classified using the National Healthcare Safety Network (NHSN) wound class. Hysterectomy is a clean-contaminated procedure and as a result, is unavoidably associated with a relatively higher risk of infection as the procedure breaches the genital tract. Common sites of infection after hysterectomy include the abdominal wall, the vaginal cuff, bladder, and pelvic floor. Related complications include pelvic abscess or infected hematoma and sepsis. A patient’s individual susceptibility to infection depends on a variety of factors including bacterial virulence, extent of surgery-related tissue trauma and fluid collection, the effectiveness of the patient’s immune system, age, nutritional status, presence of diabetes, smoking, coexistent infection or colonization with microorganisms. Perhaps the most important factors in SSI prevention in hysterectomy are timely administration of appropriate preoperative antibiotics and meticulous surgical technique. Use of β-lactam alternatives in patients who do not report an anaphylactic reaction can lead to increased antimicrobial resistance. In fact, a retrospective cohort study involving over 21,000 women undergoing hysterectomy demonstrated that the use of standard β-lactam antibiotics had a lower risk of SSI compared to those who received an alternative regimen [23]. Thus, we advise judicious use of β-lactam alternatives for patients with a history of IgE-mediated penicillin hypersensitivity. The most common organisms isolated from vaginal cuff infections are anaerobes. In a large retrospective cohort study with over 18,000 patients undergoing hysterectomy of any type, those receiving cefazolin or a second-generation cephalosporin have more than double the SSI risk compared with those receiving combined treatment with cefazolin and metronidazole [25]. This is likely related to enhanced anaerobic coverage with the addition of metronidazole. We recommend that all patients undergoing hysterectomy receive metronidazole in addition to the standard intraoperative antibiotics.
The CDC also advises that the entire body be cleansed with either soap or antiseptic the night prior to the procedure. Intraoperatively, alcohol-based chlorhexidine is more effective for skin preparation when compared to iodine solutions [30, 31]. With regards to vaginal preparation, either povidone-iodine or chlorhexidine gluconate (4%) with a low concentration of isopropyl alcohol is acceptable, as both significantly reduce rates of postoperative infectious morbidity [32].
In general, our practice will have patients return for short-term postoperative evaluation within 2 weeks following their hysterectomy. Patients are counseled to maintain pelvic rest for a minimum of 8 weeks. Postoperative blood and other secretions from the vaginal cuff may raise the vaginal pH and as a result, increase the risk of bacterial vaginosis. Many patients with vaginal cuff infections present more than 2 weeks following hysterectomy, which suggests a late ascending spread of vaginal microorganisms. As a result, our patients return for a second postoperative appointment and vaginal cuff check approximately 4–6 weeks after their hysterectomy.
Gynecological surgical site infections are polymicrobial with a mix of both anaerobic and aerobic infections. Common pathogens contain gram-negative bacilli, enterococci, streptococci, and anaerobes
Wound exploration and debridement are pillars in the management of superficial and deep-incisional SSIs. This includes not only opening the wound, debridement of necrotic and devitalized tissue, but also involves the culture of the wound to allow for speciation of potential pathogens to assist in antibiotic therapy.
The mortality and morbidity of organ/space SSI tend to be higher than superficial or deep SSI. The primary objective in management is to achieve source control. Computed tomography and ultrasound are employed to guide placement of closed suction percutaneous drains into abscess collections when feasible. The initial approach in treatment of post-hysterectomy pelvic abscess depends on three factors: (1) hemodynamic stability, (2) abscess size, and (3) abscess location. Hemodynamically unstable patients require prompt surgical intervention and intensive care monitoring.
Patients who are hemodynamically stable with a post-hysterectomy pelvic abscess should be treated empirically with parenteral broad-spectrum antibiotics. Initial antimicrobial regimens can be tailored to subsequent culture and sensitivity results. If the patient does not respond within 48–72 hours, percutaneous drainage or infectious disease consultation may be warranted. An argument can be made for earlier percutaneous drainage. In fact, a systematic review comparing the success rates of 3 modalities of minimally invasive management of tubo-ovarian abscesses—laparoscopy, ultrasound-guided drainage and computed tomography-guided drainage
Treatment failure is defined as persistent fever, leukocytosis, pain or lack of abscess resolution. Risk factors include residual fluid collection after drainage and increasing patient age. Surgical management is recommended at this time.
The most common reason for unplanned readmission after surgery is surgical site infection. SSIs are associated with increased morbidity, mortality, transfer to an intensive care setting, prolonged hospitalization, hospital readmission, and increased healthcare costs. In addition, the development of SSI negatively impacts patient experience.
The majority of postoperative issues can be anticipated and prevented preoperatively. Systematically addressing these issues at the preoperative evaluation may result in greater patient satisfaction and fewer complications. Thus, prevention of SSI after hysterectomy begins with a calculation of perioperative risk followed by addressing those risk factors prior to the procedure. Intraoperative measures aimed at SSI prevention include the implementation of evidence-based SSI prevention bundles, proper administration of intraoperative antibiotic prophylaxis, and proper skin/vaginal preparation. Postoperatively, hysterectomy patients should be followed closely.
Thanks to the faculty, residents, fellows, and medical students of the Zucker School of Medicine.
Our business values are based on those any scientist applies to their research. The values of our business are based on the same ones that all good scientists apply to their research. We have created a culture of respect and collaboration within a relaxed, friendly, and progressive atmosphere, while maintaining academic rigour.
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Integrity - We are consistent and dependable, always striving for precision and accuracy in the true spirit of science.
\n\nOpenness - We communicate honestly and transparently. We are open to constructive criticism and committed to learning from it.
\n\nDisruptiveness - We are eager for discovery, for new ideas and for progression. We approach our work with creativity and determination, with a clear vision that drives us forward. We look beyond today and strive for a better tomorrow.
\n\nIntechOpen is a dynamic, vibrant company, where exceptional people are achieving great things. We offer a creative, dedicated, committed, and passionate environment but never lose sight of the fact that science and discovery is exciting and rewarding. We constantly strive to ensure that members of our community can work, travel, meet world-renowned researchers and grow their own career and develop their own experiences.
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The results of the study are addressed to the Ministry of Economic Development of the Russian Federation in order to justify the amount of federal targeted investment programs financing in Russian regions.",book:{id:"6703",slug:"statistics-growing-data-sets-and-growing-demand-for-statistics",title:"Statistics",fullTitle:"Statistics - Growing Data Sets and Growing Demand for Statistics"},signatures:"Burtseva Tatiana Alexsandrovna",authors:null},{id:"56460",title:"Application of Taguchi-Based Design of Experiments for Industrial Chemical Processes",slug:"application-of-taguchi-based-design-of-experiments-for-industrial-chemical-processes",totalDownloads:3190,totalCrossrefCites:24,totalDimensionsCites:48,abstract:"Design of experiment is the method, which is used at a very large scale to study the experimentations of industrial processes. It is a statically approach where we develop the mathematical models through experimental trial runs to predict the possible output on the basis of the given input data or parameters. The aim of this chapter is to stimulate the engineering community to apply Taguchi technique to experimentation, the design of experiments, and to tackle quality problems in industrial chemical processes that they deal with. Based on years of research and applications, Dr. G. Taguchi has standardized the methods for each of these DOE application steps. Thus, DOE using Taguchi approach has become a much more attractive tool to practicing engineers and scientists. And since the last four decades, there were limitations when conventional experimental design techniques were applied to industrial experimentation. And Taguchi, also known as orthogonal array design, adds a new dimension to conventional experimental design. 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The area covers many techniques that offer solutions to emerging problems in robotics and enterprise-level software systems. Collaborative intelligence is highly and effectively achieved with multi-agent systems. Areas of application include swarms of robots, flocks of UAVs, collaborative software management. Given the level of technological enhancements, the popularity of machine learning in use has opened a new chapter in multi-agent studies alongside the practical challenges and long-lasting collaboration issues in the field. It has increased the urgency and the need for further studies in this field. We welcome chapters presenting research on the many applications of multi-agent studies including, but not limited to, the following key areas: machine learning for multi-agent systems; modeling swarms robots and flocks of UAVs with multi-agent systems; decision science and multi-agent systems; software engineering for and with multi-agent systems; tools and technologies of multi-agent systems.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/27.jpg",hasOnlineFirst:!1,hasPublishedBooks:!1,annualVolume:11423,editor:{id:"148497",title:"Dr.",name:"Mehmet",middleName:"Emin",surname:"Aydin",slug:"mehmet-aydin",fullName:"Mehmet Aydin",profilePictureURL:"https://mts.intechopen.com/storage/users/148497/images/system/148497.jpg",biography:"Dr. Mehmet Emin Aydin is a Senior Lecturer with the Department of Computer Science and Creative Technology, the University of the West of England, Bristol, UK. 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Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. 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Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. Currently, proteomics relies mainly on mass spectrometry (MS) combined with electrophoretic (1 or 2-DE-MS) and/or chromatographic techniques (LC-MS/MS). MS is an excellent tool that has gained popularity in proteomics because of its ability to gather a complex body of information such as cataloging protein expression, identifying protein modification sites, and defining protein interactions. 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