Factor effects on the percentage of weeds cut.
\r\n\t1. Emphasizing the unique power of the molecular docking method in new drug discovery;
\r\n\t2. Demonstration of how the molecular docking technique has led to the discovery of new molecules in cancer therapy, proteasome, and STAT3 inhibition, and the treatment of Alzheimer's disease;
\r\n\t3. Underlining the importance of molecular docking-based modeling methods in the various branches of biotechnology
\r\n\tWe hope that this book will be a common point where researchers working in the fields of life sciences and drug development will eventually meet.
",isbn:"978-1-80356-468-5",printIsbn:"978-1-80356-467-8",pdfIsbn:"978-1-80356-469-2",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!1,isSalesforceBook:!1,isNomenclature:!1,hash:"8c918a1973786c7059752b28601f1329",bookSignature:"Dr. Erman Salih Istifli",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11451.jpg",keywords:"Protein-Ligand Interaction, Lead Discovery, Molecular Recognition, Enzyme-Ligand Interaction, Mutant Enzymes, Alanine Screening, Proteasome Inhibitors, Signal Transducers, Transcription Activators (STATs), DNA Recognition Motifs, Neoplastic Cells, Amyloid-Beta Proteins",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"March 3rd 2022",dateEndSecondStepPublish:"May 4th 2022",dateEndThirdStepPublish:"July 3rd 2022",dateEndFourthStepPublish:"September 21st 2022",dateEndFifthStepPublish:"November 20th 2022",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"2 months",secondStepPassed:!0,areRegistrationsClosed:!0,currentStepOfPublishingProcess:4,editedByType:null,kuFlag:!1,biosketch:"A multidisciplinary researcher working in the fields of cytogenetics, molecular genetics, and bioinformatics-based molecular modeling (currently on the structural biology of COVID-19 and the treatment of Alzheimer’s disease). 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During his period of doctoral research, he joined the molecular cytogenetics group at the Max Planck Institute for Molecular Genetics in Berlin, Germany, and he focused there on investigating the molecular cytogenetic causes of some human rare diseases. During these studies, he contributed experimentally to the identification of four candidate genes (GRIA2, GLRB, NPY1R, and NPY5R) responsible for intelligence and obesity. He was assigned as an expert and rapporteur on eight candidate projects in the Marie-Sklodowska Curie-Actions Innovative Training Networks in 2016. In 2017, he completed the online theoretical and practical course 'Introduction to Biology - The Secret of Life', run by the Massachusetts Institute of Technology (MIT) on the edX platform. In April 2019, within the framework of Erasmus+ staff mobility program, he gave seminars on 'DNA microarrays and their use in genotoxicity' at Tirana University in Tirana, Albania. 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From chapter submission and review, to approval and revision, copy-editing and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. I maintain constant and effective communication with authors, editors and reviewers, which allows for a level of personal support that enables contributors to fully commit and concentrate on the chapters they are writing, editing, or reviewing. I assist authors in the preparation of their full chapter submissions and track important deadlines and ensure they are met. I help to coordinate internal processes such as linguistic review, and monitor the technical aspects of the process. As an ASM I am also involved in the acquisition of editors. 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Modern greenhouse bioproduction systems are required to exhibit integration of automation, biological culture practices, and control systems through the concept of Automation-Culture-Environment-oriented SYStems analysis (ACESYS) as defined in [2, 3]. The growth condition for Solanaceae vegetables in the greenhouse provides the leeway for the growth of other plants as well. In greenhouse cultivation of Cucumber (
The use of labor force that manually pulls out the weeds is still practiced by local growers. This is, however, not an efficient method since the availability of the skilled workforce that accepts repetitive tasks in the harsh greenhouse and field conditions impose uncertainties and timeliness costs [13]. It is, therefore, necessary to select a proper method for effective weed control. The trends in the agricultural robotics in the past 10 years show that automation of plant trimming with simultaneous localization and mapping techniques will change the industry in future [14]. The available time, labor, equipment, costs, and types of weeds and the areas infested need to be considered when planning a weed control program. In this regard, agricultural robotic and automation technology plays an essential role in improving the interactions between human, machine, and plants [15]. For example, the prevention of musculoskeletal disorders in manual harvesting operations in Dutch greenhouses has motivated various researchers for replacement of human labor by automatons robot for picking cucumber [16] and sweet pepper [13] fruits. Automation is a viable and sometimes necessary method to ensure maximum profits with minimum costs. In fact, one of the main purposes of agricultural automation has been always concerned with the substitution of human workforce by robots or mechanized systems that can handle the tasks more accurately and uniformly at a lower cost and higher efficiency [17, 18, 19, 20, 21, 22].
Research and development in agricultural robotics date back to 1980s, with Japan, the Netherlands, and the USA as the pioneer countries. Example of such research works included the works of [7, 23] for robotic weed control and automated harvesting of tomato. Development of an autonomous weeding machine requires a vision system capable of detecting and locating the position of the crop. Such vision system should be able to recognize the accurate position of the plant stem and protects it during the weed control [24]. A near-ground image capturing and processing technique to detect broad-leaved weeds in cereal crops under actual field conditions has been reported in the work of [25]. Here, the researchers proposed a method that uses color information to discriminate between vegetation and background, while shape analysis techniques were applied to distinguish between crop and weeds. Shape features of the radish plant and weed were investigated by [26]. They proposed a machine vision system using a charge coupled device camera for the weed detection in a radish farm resulting 92% success rate of recognition for radish and 98% for weeds.
A combined method of color and shape features for sugar beet weed segmentation was proposed by [27] with 92% success rate in classification. This rate increased to 96% by adding two shape features. Another approach extracted a correlation between the three main color components R, G and B, which constitute weeds and sugar beet color classes by means of discriminant analysis [28]. Their method resulted in different classification success rates between 77 and 98%. The segmentation of weeds and soybean seedlings by CCD images in the field was studied by [29]. Texture features of weed species have been applied for distinguishing weed species by [30] with grass and broadleaf classification accuracies of 93 and 85%, respectively. Textural image analysis was used to detect weeds in the grass [31]. Gabor wavelet features of NIR images of apples were extracted for quality inspection and used as input to kernel PCA [32]. Kernel PCA first maps the nonlinear features to linear space, and then, PCA is applied to separate the image Gabor wavelet (5 scales and 8 orientations) combined with kernel PCA that had the highest recognition rate (90.5%). Improvements in vision-based control system [13, 33, 34, 35, 36] have enabled several applications of robotic manipulators for greenhouse and orchard tasks and have contributed to the decrease in workload and labor’s fatigue, while improving the efficiency and safety of the operations. These achievements were considered a challenge in the earlier agricultural robotics works [23, 37, 38]. For example, spray equipment for weed control has been developed with vertical spray booms that increase the deposition in the canopy [39, 40, 41]. Some of these alternatives are self-propelled vehicles such as Fumimatic® (IDM S.L, Almería, Spain) and Tizona (Carretillas Amate S.L., Almería, Spain), or autonomous vehicles such as Fitorobot (Universidad de Almería, Cadia S.L., Almería, Spain) that have been designed specifically to move without difficulty over loose soils and in spaces with a large number of obstacles [41]. These vehicles rely on inductive sensors to follow metal pipes buried in the soil. Few studies have addressed the navigation problem of vehicles in greenhouses operating completely autonomously [9, 11, 15]. The main challenge of these systems is that localization approaches needed for feeding the closed-loop controllers would lead to inaccurate measurements after a few steps fail for long trajectories [42]. A stereovision system along with an image processing algorithm was used to recognize the weeds and also to estimate their location in the field. In order to experiment with vision sensors and agricultural robots, [13] created a completely simulated environment in V-REP, ROS, and MATLAB for improvement of plant/fruit scanning and visual servoing task through an easy testing and debugging of control algorithms with zero damage risk to the real robot and to the actual equipment. In another study, [43] designed a field survey mobile robot platform based for navigating inside greenhouses and open-field cultivation for automated image acquisition. A functional model shown in Figure 1 was introduced by [44] in the field test of an autonomous robot for deleafing cucumber plants grown in a high-wire cultivation system. This model was also adapted and used by [13] for the robotic harvesting of sweet pepper and on a greenhouse field survey mobile platform [43]. Artificial neural networks have also been used by many researchers to discriminate weeds [45, 46] with machine vision as shown in Figure 2. A fixed-position weed robot was presented by [47], which is interfaced to a standard belt-conveyor displacement system and provides the robot with pallets containing the crops. These reviews indicate that a commercial robotic platform for the elimination of weeds in a cucumber greenhouse has not been materialized yet. In addition, most of the research works in the area of robotic weed control are applicable prior to the plant growth or in some cases when the main plant height is between 0.2 and 0.3 m.
Task sequence during leaf picking of cucumber, adapted from [
Mechatronic paradigm followed in this research, adapted from [
The overall objective of this study was to design and develop an affordable robotic weed control system for application in greenhouse cultivation of cucumbers where plants can reach to a height of 10 m. Our design is based on mechanical weed removal techniques without using chemical materials. The specific objectives were to determine (i) the best blade design for cutting the weeds among cultivation rows, (ii) the best blade rotation (BR) speed, and (iii) the best arm motor (AM) speed.
A flowchart of the methodology is shown in Figure 3. A prototype robot was designed using AutoCAD software 2011 v18.1 (Autodesk Inc., San Rafael, CA, USA). Schematic views of the prototype robot, as well as the corresponding dimensions and parts are shown and illustrated in Figures 4 and 5. The main mechanical components of the robotic platform consist of a monorail, main chassis, ball bearings, wheels, arms, blade, and adjusting mechanism. Major electrical components include DC motors, microswitches, a 12 V 7.2–9 amp sealed lead acid battery, SRF05 ultrasonic sensors, pic 18F4550 microcontroller, and 2 × 24 LCD monitor (Figure 6). We began with the design of a monorail that was responsible to support the robot navigations and stops between two cucumber rows inside the greenhouse. The monorail has a width of 0.06 m and was placed 0.4 m above the ground (Figure 5A and B). The algorithm for robot navigation between two consecutive stops points on the monorail is also illustrated in the flowchart of Figure 5. Right after the robot is switched on, it starts moving on the monorail that is fixed along the greenhouse from one row to another. Upon reaching the first stopper point on the rail, the robot strikes the first microswitch, which sends a deactivation signal to the first motor responsible for moving the robot. While stopped between two cucumber plant rows, the robot scans for weeds and determines the distance between the detected weed and the blade arm using the ultrasonic sensors. Subsequently, a command signal is sent to the arm motor and blade motor for activating the blade rotation as illustrated in the flowchart of Figure 5.
Flowchart of the research methodology.
The CAD model design of the weed control robot.
The mechanism and flowchart for the robot navigation and control on the monorail showing (A) the monorail layout, (B) the robot mounted on the monorail, (C) the robot main body and manipulator arm, and (D) the joint setup between the robot and the monorail.
Major electrical module and wiring connections of the weed control robot.
The moving mechanical arm consists of a chassis, a small arm, and the main arm. Two main criteria were considered in designing the robot frame including minimum weight (for increasing the motor efficiency), and strength (for standing vibrations). The frame was made from an iron band bearing with the dimensions of 0.02 × 0.18 × 0.005 m. In order to provide support for the battery, bearing bases, microswitches, and the main arm, we installed additional extensions to the frame in a way that the robot gravity center is placed on the monorail. The battery is the heaviest part of the robot and can power the robot for 2 h. It was installed on the central frame above the rails and wheels. The battery weight creates stability for the robot when the main arm is outstretched, and this weight and location for the battery can hold the spinning wheel implemented in place. We placed several holes on the frame to facilitate the installation of the motor, wheels, and the required electrical fragments (Figure 5C and D). The robot makes use of four ball bearings of diameter 0.02 m, out of which three were used to hold the robot to the rail and to facilitate a smooth movement (two bearings were placed on the right and one on the left side). The fourth bearing was used to act as the second wheel for the robot. All the ball bearings have a diameter of 0.02 m and are installed on the central frame. The diameter of the robot main wheel is 0.04 m, and the ideal speed was determined using trial and errors and time-motion studies during the conducted tests. The arm frame is made of an iron band bearing with a dimension of 0.02 × 0.2 × 0.005 m. A blade was installed on the main arm that moves forward and enables robot access to the weeds between the main plants. A shank protector in one of the holes in the arm frame makes the movement and the selection of the angle for smooth cutting.
Major electronic components of the robot are three sets of SRF05 ultrasonic sensors, a PIC18F4550 microcontroller, and a 2 × 24 LCD monitor (Figure 6). The ultrasonic sensors were placed in a row having 0.10 m distance from each other. The sensors are specially positioned in a way that they cover the space between two cucumber plants on the cultivation row. As mentioned earlier, upon receiving a signal indicating weed existence, the microcontroller program determines the distance between the weed and the sensors and whether the weed is on the left, right, or middle of the sensors. This signal activates the cutting mechanism. Finally, the information of the entire process, including the distance between weed and sensors, and the specific sensor that identified the weed are shown on the robot LCD. During the experimental phase, we considered several improvements and adjustment on the sensing part and corresponding microcontroller program. For example, we used a tube pipe cover for each of the ultrasonic sensors to change the circular waves to linear waves. This was necessary because sound waves that broadcast from transmitters of ultrasonic sensors are circular. When these sensors are close to the ground, the broadcasting waves that bounce off from the ground are misinterpreted as weeds.
The robot movements are supported by three 12 V, 0.89 A DC motors that are labeled for this paper by motor 1, 2, and 3. The first motor was fixed directly to the wheels in front of the robot and was responsible for the robot movement on the monorail. To select the optimum speed for the robot, six motor speeds of 30, 40, 50, 60, 80, and 120 rpm were tested. We found that the motor with 60 rpm, 1.358 N·m torque, 12 V, 0.89 A had the best performance in the greenhouse under study. The second motor was connected to the small arm and is responsible to rotate the big arm that moves the blade of the robot at a selected speed of 10 rpm and torque of 8.15 N·m. The third motor was fixed to the frame of the main arm for rotating the blade at a high speed of 3500 rpm for efficient weed cutting and removal. This frame can move up and down and can fix the distance between the blade and the ground level. It should be noted that the 3500 rpm blade rotation speed and the 10 rpm arm motor speed were found from the experiments.
Three types of blade, namely the S-shaped, the triangle-shaped, and the circular-shaped blade (Figure 7) were initially considered in the weed cutting experiments. We conducted several tests to find the best blade width (equal to 0.1 m) for matching the 0.4 m distance between two cucumber plants. Based on our field tests, we found that the S-shaped blade was the most efficient design for the purpose of weed cutting. The blade was built from double stainless steel material to resist the corrosion in high humidity greenhouse environment. Analysis and calculations were carried out for finding the blade tip speed and corresponding vector components according to the formulations given in [48]. The corresponding diagrams of this analysis are shown schematically in Figure 7. It can be observed from Figure 7A that the direction of the tip of the blade follows a cycloid curve on the ground level. The component of blade speed in the direction of robot forward speed vector, as well as the demonstration of vector gradient in the blade speeds, is shown in Figure 7B–D. Here, WB is the circular speed of the blade (rad/s),
Design of the cutting blade, (A): calculating the velocity of rotating blade in stickles, (B): component of blade speed in direction of moving U, (C, D): calculating U and V, (E): components of forces, and (F): vector demonstration of the blade speed. Adapted from [
The weed control robot was tested in a 5000 m2 greenhouse in Jiroft city (28°40′41″N 57°44′26″E) located to the south of Kerman province of Iran (Figure 8). We planted over 10,000 cucumber seeds in pots and placed them in the greenhouse with spaces between the two plants being 0.4 m. It should be noted that in order to manually remove the weeds from 1 ha of the greenhouse under study, four seasonal workers had to perform the task every day, for 8 months (equivalent to 832 man/hour). Three experiments were conducted at different growth stages as follows: (i) during the seedling and germination stage, 15 days after the crop was cultivated and the surrounding weeds were also 15 days old (these weeds usually have thin and very flexible stalks and are 10 cm high), (ii) during the vegetation and early fruiting stage, when the cucumber plants were 2 months old, and (iii) during the mature fruiting stage, when the plants were at their mature height. Three types of blades were selected, namely the S-shaped, triangular-shaped, and circular-shaped blade. For each blade, we assigned three blade rotation (BR) speeds of BR1 = 3500, BR2 = 2500, and BR3 = 1500 rpm with two arm motor (AM) speed of AM1 = 10 and AM2 = 30 rpm. A factorial design with two-way analysis of variance (ANOVA) was used to determine variation effects in the cutting weed performance of each blade due to BR speed, AM speed, and their interaction. For the kth blade type, under the ith level of blade speed and the jth level of arm speed factor, the two-way ANOVA model was stated as
Outside and inside views of the experimental site (top), and corresponding factorial design of experiment (bottom) for determining the best combination of blade type, blade speed, and arm speed.
Results of statistical analysis are summarized in Tables 1–3 showing that the effects of blade type (T), blade rotation (BR) speed, and arm motor (AM) speed are significant at the 0.05 level. Moreover, it was found that the S-shaped blade with a mean (μ) of 67.8% and standard error (σ) of 3.052% had the highest effect, and triangular-shaped blade with μ = 61.38% and σ = 3.083% had the lowest effect on the percentage of the weeds cut (PWC). The BR factor was significant at P < 0.05, indicating that blade rotation speed of 3500 rpm with μ = 78.23% and σ = 1.71% had the highest effect and the 1500 rpm with μ = 50.39 and σ = 1.86% had the lowest effect. The AM speed factor was also found to be significant at P < 0.05, which indicates that the speed of 10 rpm with μ = 69.1% and σ = 2.45% had the highest effect and the speed of 30 rpm with μ = 59.8% and σ = 2.46% has had the lowest effect on the PWC. It was found that (Table 1) different blade shapes with the AM speed of 10 rpm had a significant effect on the PWC. While the mean PWC by the S-shaped blades was the highest, increasing AM speed to 30 rpm reduced the efficiency of the S-shaped blade (as well as with the other two blades), resulting a mean PWC of 59.39%. According to the P-values in Table 2, while all of the main effects of blade type, BR, and AM speeds are significant at 0.05 level, their interactions were not found to have a significant effect on the PWC. The results provided in Table 3 show that the difference between the two blades, S-shape and triangular shape is significant at the 0.05 level. In other words, the mean of weeds cut by these two blades are significantly different, and according to the mean differences column, the mean of the PWC by the S-shape blade is larger than the PWC by the triangular-shaped blade. The mean difference between the S-shaped and the circular-shaped blade with P-value of 0.036 is also significant at the 0.05 level. This implies that the average PWC by these two blades are significantly different, and according to the mean differences column, the mean PWC by the S-shaped blade is larger than the mean of the PWC by the circular-shaped blade. It was found that the difference between the means of the triangular-shaped blade and circular-shaped blade with the P-value of 0.076 is not significant at the 0.05 level, that is, the mean of the PWC by these two blade types are not significantly different.
Blade type | μ: Mean percentage of weeds cut (%) | σ: Std. error (%) |
---|---|---|
A: S shaped | 67.8 | 3.05 |
B: Triangular shape | 61.38 | 3.08 |
C: Circular shape | 64.3 | 3.38 |
Blade rotation speed (rpm) | ||
1500 | 50.3 | 1.86 |
2500 | 64.9 | 1.51 |
3500 | 78.2 | 1.71 |
Arm motor speed (rpm) | ||
10 | 69.148 | 2.457 |
30 | 59.88 | 2.461 |
Factor effects on the percentage of weeds cut.
Model | Sum of squares | Mean sum of squares | P-value |
---|---|---|---|
Blade type (T) | 110.3 | 110.3 | 0.0462 |
Blade rotation speed (BR) | 6977.1 | 3488.6 | 0.000 |
Arm motor speed (AM) | 1157.4 | 1157.4 | 0.000 |
Error | 1264.6 | 26.3 | |
Interaction types | P-value | ||
T × BR | 114.1 | 57.1 | 0.1272 |
T × AM | 5.4 | 5.4 | 0.6493 |
BR × AM | 73.6 | 36.8 | 0.2560 |
T × BR × AM | 16 | 8 | 0.7356 |
Variance analysis and effects of the robot blade type (T), blade rotation (BR) speed, and arm motor (AM) speed on the percentage of weed cutting performance.
Mean differences | P-value | |
---|---|---|
Blade type | ||
A-B | 6.4444 | 0.000 |
A-C | 3.5000 | 0.036 |
B-C | −2.9444 | 0.076 |
Blade rotation (rpm) | ||
BR1-BR2 | −14.5556 | 0.000 |
BR1-BR3 | −27.8333 | 0.000 |
BR2-BR3 | −13.2778 | 0.000 |
Comparison of significant difference between blade types (A: S-shape, B: Triangular shape, and C: Circular shape), and blades rotation speed (BR1: 1500, BR2: 2500, and BR3: 3500 rpm).
Results of analysis of variance also showed that the mean differences between the BR speeds are significant, indicating that the resulted PWC with BR1 = 1500, BR2 = 2500, and BR3 = 3500 rpm are not equal. More specifically, the PWC in 1500 rpm was found to be smaller than those of 2500 and 3500 rpm. In addition, the mean PWC in 2500 rpm was also smaller than that of 3500 rpm. This can also be observed from the bar plots of Figure 9, showing that the mean PWC in 1500 rpm is the smallest (59.39%) and that of 3500 rpm was the largest (78.23%). The bar plots in Figure 10 illustrate descriptive statistics and frequency of the PWC for the experiments with the robot using all factors (blade types A, B, C, blade rotation speeds of 1500, 2500, 3500 rpm, and arm motor speed of 10 and 30 rpm). It can be seen from Figure 10 that the average PWC by the blades was significantly different. Consequently, the highest PWC cut was related to S-shaped at the blade rotation speed of 3500 rpm. In each motor arm speed, the increase in the rotational blade speed caused an increase in the PWC. In each rotational blade speed, if the motor arm speed increases, the PWC cut will decrease. Comparing the interactions between the three different types of blades, blade speed, and the speed of the arm the following results was obtained: the highest PWC in the entire experiment was 95%, which was obtained when the S-shaped blade at the rotational speed of 3500 rpm was used and motor speed was 10 rpm. The lowest PWC was 45%, which was obtained when the blade speed was 1500 rpm, AM speed was 30 rpm, and the blade type was triangular in shape. The analysis of the interaction of the BR speed and blade type showed that (i) none of the mutual interactions was significant in the variance test, (ii) t-test showed that if the rotational speed of the blade is low, the blade type will have a significant effect on the PWC, and (iii) for all the blade types, the highest PWC cut was at BR speed of 3500 rpm.
Comparison of the effects of various blade types on (left) and various blade rotation speeds (right) on the percentage of weeds cut.
Bar plots describing percentage of weeds cut with different blade type, blade rotation speed, and robot arm speed.
In this study, we designed, developed, and fabricated a prototype robot for mechanical weed control in greenhouse cultivation of cucumber. Automatic weed cutting experiments that were carried using the robot consist of ultrasonic sensor, which senses the existence of weeds between the cucumber plants. The robot then moves between cucumber rows on a monorail in the greenhouse, with an arm that moves the blade between the plants for cutting the detected weeds. The entire process of weed detection, moving the arm and blades, and weeds cutting is carried out in 10 s. Among the three blade types tested (S-, triangular-, and circular shapes), it was concluded that the S-shape was the most efficient design. For the best blade rotation (BR) and arm motor (AM) speeds, it was concluded that as the AM speed increased, the percentage of weeds cut (PWC) reduces; therefore, the motor with 10 rpm, 8.15 N·m torque, 12 V, and 0.89 A was selected to for moving the arm. The average weeds cut at 10 and 30 rpm was 69.1 and 58.9%, respectively. Finally, it was concluded that the best robot performance corresponding to the highest percentage of weeds cut was achieved with the S-shaped blade when the BR speed was 3500 rpm, and the AM speed was 10 rpm.
The authors declare no conflict of interest.
Achieving and maintaining hemostasis is essential across all subspecialties of surgery [1, 2, 3, 4, 5, 6]. Direct application of pressure, suture ligation, and electrocautery may sufficiently control bleeding during straightforward procedures, however, such methods are often ineffective in hemodynamically unstable, coagulopathic or septic patients [6, 7, 8]. Biosurgical hemostats are increasingly important in facilitating hemostasis when standard measures prove inadequate, or in situations such as prehospital trauma [7, 9]. Biosurgical materials (BSM) are available in cotton-like, powder, patch, liquid, and glue format, and are classified based on their properties and interactions within the coagulation cascade [5, 10, 11]. The goal of this chapter is to review the many intricacies and molecular physiology of hemostasis, and to discuss the current role for biosurgicals within the overall operative strategy, focusing on applications of BSMs in acute care surgery and trauma.
The authors performed an exhaustive medical literature search utilizing PubMed and Google Scholar™ platforms. The following terms were utilized, alone or various combinations: “acute care surgery,” “biosurgical hemostat,” “biosurgical material,” “bleeding,” “coagulopathy,” “emergency surgery,” “hemorrhage,” “hemostasis,” “injury,” “management,” “non-surgical bleeding,” “surgery,” “transfusion,” “trauma.” Additional references identified during the primary literature search were subsequently reviewed and added. From more than 27,781,100 citations, we narrowed down the candidate study list to approximately 1400. The final reference list included the 90 results most relevant to the current chapter.
The multiprotein coagulation cascade consists of two separate pathways, the contact mediated (intrinsic) and tissue factor (extrinsic) pathway which together unite to activate thrombin and form the fibrin plug (Figure 1) [12, 13]. Many of the anticoagulant medications focus on altering the steps herein, and the inherited coagulopathic disorders also feature abnormalities at various steps in the pathway [13, 14]. BSMs also interact here, as the so-called
Overview of coagulation cascade. Diagram of the multistep intrinsic (left, blue) and extrinsic pathway (right, green). Whether initiated by surface contact or tissue damage, both pathways combine into the common pathway leading to activation of factor X and then subsequent thrombin-fibrin activation and finally formation of the fibrin clot. Legend demonstrates where hemostatic agents, mechanical, and adhesive hemostats exert their roles in the coagulation cascade.
Adhesive agents, whether liquid or fibrin patch, contain thrombin and fibrin thereby facilitating the final steps of the coagulation cascade [5, 11]. These added factors may help facilitate hemostasis in patients with various functional impairments within the coagulation cascade [15, 16]. Alterations within the coagulation mechanism that lead to the so-called “non-surgical bleeding” may be due to coagulopathy of massive traumatic hemorrhage, large intraoperative blood losses, or sepsis secondary to gastrointestinal perforation [8, 17, 18, 19, 20]. Within the broader context of surgical “damage control” emerges perhaps the most compelling use case for BSMs [21, 22].
Historically, adhesive preparations contained additional antifibrinolytic components such as tranexamic acid (TXA) and aprotinin, which are no longer utilized due to reported side effects in cardiac surgery, including anaphylaxis, renal failure, increased mortality (aprotinin), and neurotoxicity (tranexamic acid) [23, 24, 25]. There are several liquid formulations of adhesive hemostats and “patch” alternatives available today (Table 1) [5]. Liquid adhesives are easily applied to delicate tissue, while fibrin patch alternatives can be more bluntly applied to brisk hemorrhage thereby providing improved hemostasis for various tissue types [5]. Among available products, different brands have their own unique fibrinogen/thrombin ratio affecting clot strength and drying time [5, 9, 26]. In general, higher thrombin concentrations correlate with the rate of clot formation whereas increased fibrinogen levels are associated with overall clot strength, which is not surprising as thrombin functions more proximally to fibrinogen, acting as a limiting reagent in the coagulation cascade, whereas fibrinogen more directly affects fibrin levels within the fibrin plug (Figure 1) [27, 28, 29, 30, 31, 32, 33].
Adhesive | ||||
---|---|---|---|---|
Name | Manufacturer | Mechanism | Form | Comment |
Beriplast | CSL Behring LLC King of Prussia, PA, USA | Thrombin/fibrin | Liquid | Hemostasis on native and PTFE surfaces |
Evicel | Omrix LTD Ness Ziona, IL, USA | Thrombin/fibrin | Liquid | Hemostasis on native and PTFE anastomosis, help with dural suture lines |
Floseal | Baxter Inc. Deerfeild, IL, USA | Thrombin/fibrin+porcine gelatine | Liquid | |
Qubeil | Omrix LTD Ness Ziona, IL, USA | Thrombin/fibrin | Liquid | |
Surgiflo | J & J Healthcare Hacketstown, NJ, USA | Thrombin/fibrin+porcine gelatin | Liquid | |
Tisseal | Baxter Inc. Deerfeild, IL, USA | Thrombin/fibrin | Liquid | Hemostasis on native and PTFE surfaces |
Evarrest | Omrix LTD Ness Ziona, IL USA | Thrombin/fibrin | Patch | Patch provides added compression |
Tachosil | Baxter Inc. Deerfeild, IL USA | Thrombìn/fibrin+equine collagen patch | Patch | Patch provides added compression, hemostasis on portal and hepatic artery anastomosis |
Use when coagulation cascade impaired. Do not use in the setting of allergy to blood products. Thromboembolic risk with intravascular administration |
Adhesive hemostats.
Overview of adhesive hemostats name, manufacturer, mechanism, and form.
Clinical trials focused on controlling intrabdominal and retroperitoneal hemorrhage have demonstrated that adhesive hemostats containing fibrin/thrombin coagulation factors tend to be superior to hemostats lacking these components [5]. A number of vascular surgery studies have shown significant improvement in the rate of hemostasis on native and polytetrafluoroethylene (PTFE) arterial anastomosis with several such agents (Table 1) [25, 34, 35]. In neurosurgical applications, another thrombin/fibrin adhesive was shown effective in helping to seal dural suture line leaks [36, 37]. For abdominal applications, one type of a hemostatic honeycomb patch matrix with added fibrin and thrombin factors was shown to reduce anastomotic bleeding following portal vein repair and hepatic artery reconstruction (Table 1) [5, 38]. However, there are conflicting data on whether these hemostatic agents can reduce the incidence of “structural” (versus “hemostatic”) events, such as postoperative pancreatic fistula following distal pancreatectomy, bile leakage status post hepatectomy, and persistent air leak after thoracic surgery [5].
Mechanical hemostats typically consist of cellulose, collagen, gelatin, and other plant-based materials [39, 40, 41]. The composition of these BSMs differs from the adhesive hemostats, as they lack coagulation factors and therefore require a functioning coagulation cascade to exert their effects [5]. Their mechanism of action relies on the absorbent capacity of the material, thereby exerting mass effect on the adjacent tissue and subsequent activation of the extrinsic coagulation pathway (Figure 1) [9, 42, 43].
Oxidized cellulose based hemostats are classified according to the way they are processed (regenerated versus non-regenerated) [5]. In short, the regenerated products have a more organized structure and conform better to their surroundings in comparison to the latter. Despite this, multiple studies have demonstrated greater hemostatic capacity for the non-regenerated products [44, 45, 46]. There are numerous products based on oxidized regenerated cellulose as the primary BSM, with relatively fewer non-regenerated products (Table 2) [5, 46]. In comparison to the adhesive hemostats, mechanical products are generally easier to use, relatively less expensive, and also exhibit bacteriostatic properties due to their low pH [20, 47, 48, 49]. Even though cellulose degrades within approximately 5 weeks, one limitation to its use includes the increased formation of granulation tissue months afterwards, which may produce a mass effect on vessels with subsequent stenosis or paralysis if adjacent nerves are compressed [50, 51, 52, 53, 54]. Moreover, the postoperative course for cancer patients may become significantly more complicated if granulation tissue results in a pseudotumor following the resection of the recurrent mass [50, 51, 52, 53, 54, 55].
Mechanical | ||||
---|---|---|---|---|
Name | Manufacturer | Mechanism | Form | Comment |
Surgicel | Ethicon Somerville, NJ, USA | Oxidized celluose | Sheet | |
Surgicel Fibrillar | Ethicon Somerville, NJ, USA | Oxidized celluose | Sheet | |
Surgicel Nu-kit | J & J Healthcare Hacketstown, NJ, USA | Oxidized celluose | Sheet | |
Traumastem | Bloster Veverská Bitẏŝka, Czech Republic | Oxidized celluose | Sheet | |
Avitene | Bard Warwick, RI, USA | Collagen | Sheet/sponge/powder | Hemostasis during crainiotomy |
Gelfoarm | Pfizer NY, NY, USA | porcine gelatin | Sponge | |
Arista | Bard Warwick, RI, USA | polysaccharide spheres | Powder | Improved hemostatis in cardiothoracic surgery |
Need normal coagulation cascade. Low pH with bacteriacidal properties. Thromboembolic risk with intravascular infection. |
Mechanical hemostats.
Overview of mechanical hemostats name, manufacturer, mechanism, and form.
Collagen is a key component of extracellular membranes and, as such, a great deal of effort has been placed into bioengineering collagen-based biosurgicals (CBBs) [56]. Additionally it is thought that such hemostats would also facilitate wound healing and potentially assist in neovascularization [56]. One CBB is known to improve perioperative hemostasis during craniotomy, and similar collagen based materials were shown to improve hemostasis during gynecological procedures (Table 2) [57, 58]. A recently bioengineered product containing cellulose, chitosan, and oxidized bacterial cellulose was shown to be both bactericidal and hemostatic in an animal model of liver injury, comparing favorably to a commonly used alternative [59].
Gelatin based mechanical hemostats which are either bovine- or porcine-based, have a neutral pH permitting combination with thrombin adhesives [60]. Therefore their hemostatic function extends into two domains, including both “absorptive-mediated” activation of the extrinsic pathway in addition to facilitating the final steps of the thrombin-fibrin cascade [5, 61]. In terms of biodegradation, gelatin sponge, granule, and powder dissolves within approximately 6 weeks of placement [5]. Both gelatin- and collagen-based hemostats are effective in reducing perioperative blood loss. Additionally, neurosurgical models suggest that collagen-based products may be superior in controlling hemorrhage in spinal fusion procedures [62]. Although collagen- and gelatin-based products may be less expensive than fibrin products, one negative aspect is that when actively using blood saving techniques (e.g., cell-saver device), these products may pass through 20 micron filters leading to an inflammatory renal response [63].
Another mechanical hemostat category is derived from plant starch and is known as a microporous polysaccharide hemosphere (MPH) (Table 2) [64]. This highly absorbent product works similar to collagen and gelatin formulations through activation of the extrinsic clotting cascade. However, in comparison to collagen and gelatin, sufficient evidence exists in cardiothoracic surgery to demonstrate improved hemorrhage control, reduced chest tube output and fewer perioperative blood product transfusions [5]. From orthopedic surgery perspective, MPH may be favored as it does not inhibit bone healing compared to bone wax or microfibrillar collagen [65]. Moreover, microporous hemospheres absorb blood rapidly and have less capacity to cause infection and granuloma than other biologic hemostats [5, 66].
Sealant hemostats (SH) are a class of hemostatic polymers which form a matrix by crosslinking and interlocking protein-rich adjacent tissues. Interestingly, sealants neither require the presence of blood products nor an intact coagulation cascade to exert their effects [5]. Natural sealants are derived from proteins and polysaccharides, while synthetic and semisynthetic products are composed of polyethylene glycol cyanoacrylate (PEG), polyurethane, dendrimer, and glutaraldehyde albumin biomaterials [67, 68]. Composite sealants contain both natural and synthetic components. All have been exploited for hemostasis, wound healing, fistula repair and implant fixation in fields such as cardiothoracic, biliary, urologic, plastic, neuro, and endoscopic surgery [68].
Natural fibrin based sealants are a mixture of fibrinogen and thrombin and come in either dry or foam preparations. They are mainly used to decrease suture and staple line seepage [56, 68]. Other proteins utilized in sealant applications include collagen, keratin, albumin, as well as muscle-derived products [68]. Polysaccharide compounds include chitosan, alginates, and chondroitin sulfates [69, 70]. A recent phase III clinical trial revealed that a fibrin-based sealant can provide excellent hemostasis, potentially superior to manual compression in patients undergoing open arterial surgery [71]. Interestingly, some of the well-known fibrin-based hemostats which leverage the coagulation cascade as the primary mechanism of action also exhibit a sealant function via covalent interactions with the surrounding tissues [56]. Even though fibrin sealants may be less effective in blood-saturated tissues, the potential benefit of an added “sealant feature” provided insight into research on new hemostatic sealants characterized by increased overall strength, elasticity, and stronger covalent interactions with surrounding tissues [72]. Other limitations of fibrin sealants include increased price compared to synthetic products, as well as high reliance on human plasma products for manufacturing purposes thereby increasing the risk, albeit very low, of transmissible disease [56].
PEG based products can be prepared in both patch and liquid formats to function as both hemostatic sealants and fluid barriers (Table 3) [5, 56]. There are patch products infused with collagen or cellulose, with superior hemostatic properties (Table 3) [5, 73]. Studies show less bleeding around anastomotic suture lines in nephrectomy and coronary artery bypass procedures with the use of bovine collagen and pentaerythritol polyethylene glycol ether tetra-succinimidyl glutarate patch [44, 46]. Similarly, a combined PEG and oxidized cellulose product significantly reduced oozing in hepatectomy procedures [74]. Another related product is effective as a fluid barrier and significantly improves closure of the dura during spinal surgery [75, 76, 77]. Finally, PEG-albumin sealants appear to be able to similarly reduce air leaks following pulmonary lobectomy procedures [44, 78, 79].
Sealants | ||||
---|---|---|---|---|
Name | Manufacturer | Mechanism | Form | Other |
Hemopatch | Baxter Deerfeild, IL, USA | PEG/collagen | Patch | Data to support use in nephrectomles and CABG |
Duraseal | Integra Palinsboro township, NJ, uSa | PEG | Liquid | Improves dural closure |
Veriset | Covidien Walpole, MA, USA | PEG/cellulose | Patch | Decreased bleeding after hepatectomy |
FocalSeal | Focal Inc. Lexington, ma, USA | Eosin primer/PEG | Liquid | Decreased air leak after lobectomy |
TissuGlu | Cohera Medical Oplotnica, Solvania | Polyurethane | Liquid | |
Dermabond | Ethicon Somerville, NJ, USA | Cryanoacylate | Liquid | Exothermic reaction |
Bioglue | CryoLife Kennesaw, GA, USA | glutaraldehyde-bovine albumin | Liquid | Safe for vascular anastomosis |
Do not use with renal disease patient’s |
Hemostatic sealants.
Overview of hemostatic sealants name, manufacturer, mechanism, and form.
Polyurethane sealants have great potential and promise due to their highly elastic properties. Of importance, in an animal abdominoplasty model, this type of sealant was found to reduce seroma formation [56]. However there are safety concerns due to the potential thrombotic risk associated with similar products [56].
It is important to note that hemostatic sealants are not without risks, and their limitations include some concerns regarding the strength of the deployed material as well as some degree of interference with wound healing [68]. Although synthetic sealants have increased elasticity compared to natural counterparts, their limitations include longer curing time, the potential for chronic inflammation and the risk of cytotoxicity [56]. Moreover, PEG-based sealants should not be used in renal insufficiency as they are dependent on renal clearance following their breakdown and absorption [5]. Of note, PEG-sealants also tend to swell prior to degrading, which does provide tamponade assisting in hemostasis, but may be detrimental if applied within closed spaces that contain neurovascular structures or ureters [56]. Cyanoacrylates similar to those used in skin closure for improved cosmetic outcome, when used in large quantities, can produce significant amounts of heat and potentially lead to tissue damage [5]. Additionally, these products cannot be used intravascularly, or directly on vascular anastomoses, due to risk of embolization (in fact, the material can be utilized in therapeutic embolization and other hemostatic maneuvers) [80, 81, 82]. The glutaraldehyde-bovine albumin, on the other hand, is safe and effective to directly apply on vascular and cardiac anastomotic suture sites [5]. Moreover, the use of this type of substance was shown to reduce blood transfusion requirements postoperatively, appears to be safe on adjacent nerves, and carries only a limited risk of vascular anastomotic stenosis secondary to an inflammatory response if the product is applied circumferentially [5].
Mineral-based and polysaccharide containing hemostatic dressings are of great value for pre-hospital exsanguinating patients, especially when protective measures such as tourniquet placement are not feasible due to the anatomical location of the wound [83, 84]. Such dressings are classified based on their mechanism of action, and include factor concentrators, muco-adhesives, or procoagulants [5]. Factor concentrators composed of volcanic or clay minerals such as zeolite and smectite act by concentrating protein components of blood thereby facilitating clot formation. The most commonly used factor concentrator is composed of zeolite beads which can be poured directly into a wound to expedite hemostasis (Table 4). One downside of this product is that it releases significant amounts of heat in a highly exothermic reaction. Consequently, care must be taken to use this preparation externally only, with applications limited to quantities “as little as necessary” to stop external bleeding [5]. Muco-adhesive products consist of the polymer chitosan, a chitin based product, which is derived from crustacean exoskeleton material [85]. Interestingly, chitosan has no intrinsic hemostatic ability, but its chemical charge is opposite to erythrocytes, thus promoting hemostasis [86, 87].
Hemostatic | ||||
---|---|---|---|---|
Name | Manufacturer | Mechanism | Form | Other |
Quick clot | Z-Medica LLC Wallingford, CT, USA | Zeolite beads | Guaze | Exothermic reaction. Needs to be removed from wound. |
HemCon | Tricol Biomedical Portland, OR, USA | Chitin | Gauze | Exothermic reaction. Needs to be removed from wound. |
Quick clot Combat Guaze | Z-Medica LLC Wallingford, CT, USA | Kaolin | Gauze | Exothermic reaction. Needs to be removed from wound. |
Use externally for significant bleeding |
Hemostatic agents.
Overview of hemostatic agents separated by name, manufacturer, mechanism, and formulation.
Procoagulant hemostats work by actively facilitating the coagulation cascade. One product, known as “combat gauze,” consists of surgical dressing coated with the mineral kaolin (Table 4) [88]. Kaolin is a nonreactive mineral that activates the intrinsic, contact-mediated clotting pathway when it comes in direct contact with damaged tissue [5]. The product is non-absorbable and must be removed after hemostasis is achieved, but does not create the exothermic reaction observed in factor concentrators. Baker and colleagues demonstrated that a novel fibrin-based sealant was equally effective when compared to “combat gauze” in a porcine model of both hepatic and femoral artery injury [89]. Similarly, this novel fibrin sealant was also shown to be superior other formulations in a similar swine model of splenic laceration [90]. Further studies are ongoing in this important and rapidly developing area of clinical investigation.
The ideal surgical hemostat should be biocompatible with its target tissues, safe, easy-to-use, and capable of providing rapid hemostasis. Importantly, BSMs should be able to operate under a broad range of conditions, possibly even when applied to actively bleeding or oozing surfaces, without being washed away or immediately losing their potency. Additionally, the optimal hemostat should have sufficient strength to hold the tissues in place to accomplish tamponade and assist in further healing. In the field of trauma and acute care surgery BSMs should maintain high levels of effectiveness for at least several hours, only act on the intended target tissues, should preferably be biodegradable, and should not impair wound healing. Affordability and ease of application are also important considerations. Future product development should also aim to incorporate, whenever possible, bactericidal properties. Moreover, novel materials may perform better if components like collagen are added to also assist in subsequent wound healing and maintenance of tissue integrity. Additionally, future research should aim to further understand the various tissue-specific effects of surgical hemostats, as well as their molecular and cellular interactions in order to individualize and optimize the resultant clinical outcomes.
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In particular, many neuroimaging studies find that the amygdala fails to activate in response to negative stimuli in individuals with PTSD. Several technical and design issues may explain disparate results regarding amygdala reactivity in PTSD. However, biological and symptom-based factors emerge as possible mediators of amygdala function in PTSD, leading to the conclusion that symptoms of emotional disengagement and dissociation are associated with amygdala hyporeactivity, and symptoms of hypervigilance/hyperarousal and problems with fear conditioning and extinction are reflected by amygdala hyperactivity. Therefore, treatment of PTSD should take into account the nature of amygdala dysfunction in the individual to optimize treatment outcomes.",book:{id:"5485",slug:"the-amygdala-where-emotions-shape-perception-learning-and-memories",title:"The Amygdala",fullTitle:"The Amygdala - Where Emotions Shape Perception, Learning and Memories"},signatures:"Gina L. Forster, Raluca M. Simons and Lee A. Baugh",authors:[{id:"145620",title:"Dr.",name:"Gina",middleName:null,surname:"Forster",slug:"gina-forster",fullName:"Gina Forster"},{id:"195109",title:"Dr.",name:"Raluca",middleName:null,surname:"Simons",slug:"raluca-simons",fullName:"Raluca Simons"},{id:"195110",title:"Dr.",name:"Lee",middleName:null,surname:"Baugh",slug:"lee-baugh",fullName:"Lee Baugh"}]},{id:"55211",doi:"10.5772/intechopen.68618",title:"The Amygdala and Anxiety",slug:"the-amygdala-and-anxiety",totalDownloads:2992,totalCrossrefCites:4,totalDimensionsCites:8,abstract:"The amygdala has a central role in anxiety responses to stressful and arousing situations. Pharmacological and lesion studies of the basolateral, central, and medial subdivisions of the amygdala have shown that their activation induces anxiogenic effects, while their inactivation produces anxiolytic effects. Many neurotransmitters and stress mediators acting at these amygdalar nuclei can modulate the behavioral expression of anxiety. These mediators may be released from different brain regions in response to different types of stressors. The amygdala is in close relationship with several brain regions within the brain circuitry that orchestrates the expression of anxiety. Recent developments in optogenetics have begun to unveil details on how these areas interact.",book:{id:"5485",slug:"the-amygdala-where-emotions-shape-perception-learning-and-memories",title:"The Amygdala",fullTitle:"The Amygdala - Where Emotions Shape Perception, Learning and Memories"},signatures:"Sergio Linsambarth, Rodrigo Moraga-Amaro, Daisy Quintana-\nDonoso, Sebastian Rojas and Jimmy Stehberg",authors:[{id:"144923",title:"Dr.",name:"Jimmy",middleName:null,surname:"Stehberg",slug:"jimmy-stehberg",fullName:"Jimmy Stehberg"},{id:"194182",title:"Ph.D. Student",name:"Rodrigo",middleName:null,surname:"Moraga-Amaro",slug:"rodrigo-moraga-amaro",fullName:"Rodrigo Moraga-Amaro"},{id:"194183",title:"M.Sc.",name:"Sergio",middleName:null,surname:"Linsambarth",slug:"sergio-linsambarth",fullName:"Sergio Linsambarth"}]}],mostDownloadedChaptersLast30Days:[{id:"54675",title:"The Key Role of the Amygdala in Stress",slug:"the-key-role-of-the-amygdala-in-stress",totalDownloads:2939,totalCrossrefCites:3,totalDimensionsCites:4,abstract:"Several data highlighted that stress exposure is strongly associated with several psychiatric disorders. The amygdala, an area of the brain that contributes to emotional processing, has a pivotal role in psychiatric disorders and it has been demonstrated to be highly responsive to stressful events. Here we will review evidences indicating how the amygdala changes its functionality following exposure to stress and how this contributes to the onset of anxiety disorders.",book:{id:"5485",slug:"the-amygdala-where-emotions-shape-perception-learning-and-memories",title:"The Amygdala",fullTitle:"The Amygdala - Where Emotions Shape Perception, Learning and Memories"},signatures:"Diego Andolina and Antonella Borreca",authors:[{id:"190318",title:"Dr.",name:"Diego",middleName:null,surname:"Andolina",slug:"diego-andolina",fullName:"Diego Andolina"},{id:"192832",title:"Dr.",name:"Antonella",middleName:null,surname:"Borreca",slug:"antonella-borreca",fullName:"Antonella Borreca"}]},{id:"55211",title:"The Amygdala and Anxiety",slug:"the-amygdala-and-anxiety",totalDownloads:2984,totalCrossrefCites:4,totalDimensionsCites:8,abstract:"The amygdala has a central role in anxiety responses to stressful and arousing situations. Pharmacological and lesion studies of the basolateral, central, and medial subdivisions of the amygdala have shown that their activation induces anxiogenic effects, while their inactivation produces anxiolytic effects. Many neurotransmitters and stress mediators acting at these amygdalar nuclei can modulate the behavioral expression of anxiety. These mediators may be released from different brain regions in response to different types of stressors. The amygdala is in close relationship with several brain regions within the brain circuitry that orchestrates the expression of anxiety. Recent developments in optogenetics have begun to unveil details on how these areas interact.",book:{id:"5485",slug:"the-amygdala-where-emotions-shape-perception-learning-and-memories",title:"The Amygdala",fullTitle:"The Amygdala - Where Emotions Shape Perception, Learning and Memories"},signatures:"Sergio Linsambarth, Rodrigo Moraga-Amaro, Daisy Quintana-\nDonoso, Sebastian Rojas and Jimmy Stehberg",authors:[{id:"144923",title:"Dr.",name:"Jimmy",middleName:null,surname:"Stehberg",slug:"jimmy-stehberg",fullName:"Jimmy Stehberg"},{id:"194182",title:"Ph.D. Student",name:"Rodrigo",middleName:null,surname:"Moraga-Amaro",slug:"rodrigo-moraga-amaro",fullName:"Rodrigo Moraga-Amaro"},{id:"194183",title:"M.Sc.",name:"Sergio",middleName:null,surname:"Linsambarth",slug:"sergio-linsambarth",fullName:"Sergio Linsambarth"}]},{id:"32387",title:"The Mystery of P2X7 Ionotropic Receptor: From a Small Conductance Channel to a Large Conductance Channel",slug:"the-mystery-of-p2x7-receptor-from-a-small-channel-to-a-big-pore",totalDownloads:2418,totalCrossrefCites:0,totalDimensionsCites:0,abstract:null,book:{id:"1592",slug:"neuroscience-dealing-with-frontiers",title:"Neuroscience",fullTitle:"Neuroscience - Dealing With Frontiers"},signatures:"R.X. Faria, L.G.B. Ferreira and L.A. Alves",authors:[{id:"76663",title:"Prof.",name:"Luiz A.",middleName:null,surname:"Alves",slug:"luiz-a.-alves",fullName:"Luiz A. Alves"},{id:"76674",title:"Mr.",name:"Leonardo",middleName:null,surname:"Braga",slug:"leonardo-braga",fullName:"Leonardo Braga"},{id:"79615",title:"Dr.",name:"Robson",middleName:null,surname:"Faria",slug:"robson-faria",fullName:"Robson Faria"}]},{id:"32399",title:"Brain Energy Metabolism in Health and Disease",slug:"brain-energy-metabolism-in-health-and-disease",totalDownloads:9132,totalCrossrefCites:1,totalDimensionsCites:10,abstract:null,book:{id:"1592",slug:"neuroscience-dealing-with-frontiers",title:"Neuroscience",fullTitle:"Neuroscience - Dealing With Frontiers"},signatures:"Felipe A. Beltrán, Aníbal I. Acuña, María Paz Miró and Maite A. Castro",authors:[{id:"107041",title:"Dr.",name:"Maite A",middleName:null,surname:"Castro",slug:"maite-a-castro",fullName:"Maite A Castro"},{id:"109692",title:"Mr.",name:"Felipe A",middleName:null,surname:"Beltran",slug:"felipe-a-beltran",fullName:"Felipe A Beltran"},{id:"109695",title:"Mr.",name:"Aníbal",middleName:"I.",surname:"Acuña",slug:"anibal-acuna",fullName:"Aníbal Acuña"},{id:"109696",title:"Ms.",name:"Maria Paz",middleName:null,surname:"Miro",slug:"maria-paz-miro",fullName:"Maria Paz Miro"}]},{id:"54509",title:"The Contribution of the Amygdala to Reward-Related Learning and Extinction",slug:"the-contribution-of-the-amygdala-to-reward-related-learning-and-extinction",totalDownloads:1742,totalCrossrefCites:3,totalDimensionsCites:3,abstract:"There has been substantial research into the role of the amygdala in fear conditioning and extinction of conditioned fear. The role of the amygdala in appetitive conditioning is relatively less explored. Here, we will review research into the role of the amygdala in reward‐related learning. Research to date suggests that the basolateral and central amygdala are responsible for learning about distinct aspects of a reinforcing event. For example, the basolateral amygdala is essential for distinguishing and choosing between specific rewards based on the specific‐sensory properties of those rewards as well as updating the relative value of specific rewarding events. In contrast, the central amygdala is involved in encoding reinforcement more generally and for regulating motivational influences on responding. We will also review what is known about the role of the amygdala in extinction of reward‐related behaviours and highlight areas for future research.",book:{id:"5485",slug:"the-amygdala-where-emotions-shape-perception-learning-and-memories",title:"The Amygdala",fullTitle:"The Amygdala - Where Emotions Shape Perception, Learning and Memories"},signatures:"Rose Chesworth and Laura Corbit",authors:[{id:"193670",title:"Dr.",name:"Laura",middleName:null,surname:"Corbit",slug:"laura-corbit",fullName:"Laura Corbit"},{id:"194020",title:"Dr.",name:"Rose",middleName:null,surname:"Chesworth",slug:"rose-chesworth",fullName:"Rose Chesworth"}]}],onlineFirstChaptersFilter:{topicId:"1176",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:90,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:32,numberOfPublishedChapters:319,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:141,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:133,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:107,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:5,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:16,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. 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",coverUrl:"https://cdn.intechopen.com/series/covers/23.jpg",latestPublicationDate:"June 25th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:0,editor:{id:"280770",title:"Dr.",name:"Katherine K.M.",middleName:null,surname:"Stavropoulos",slug:"katherine-k.m.-stavropoulos",fullName:"Katherine K.M. Stavropoulos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRdFuQAK/Profile_Picture_2022-05-24T09:03:48.jpg",biography:"Katherine Stavropoulos received her BA in Psychology from Trinity College, in Connecticut, USA. Dr. Stavropoulos received her Ph.D. in Experimental Psychology from the University of California, San Diego. She completed her postdoctoral work at the Yale Child Study Center with Dr. James McPartland. Dr. Stavropoulos’ doctoral dissertation explored neural correlates of reward anticipation to social versus nonsocial stimuli in children with and without autism spectrum disorders (ASD). She has been a faculty member at the University of California, Riverside in the School of Education since 2016. Her research focuses on translational studies to explore the reward system in ASD, as well as how anxiety contributes to social challenges in ASD. She also investigates how behavioral interventions affect neural activity, behavior, and school performance in children with ASD. She is also involved in the diagnosis of children with ASD and is a licensed clinical psychologist in California. She is the Assistant Director of the SEARCH Center at UCR and is a Faculty member in the Graduate Program in Neuroscience.",institutionString:null,institution:{name:"University of California, Riverside",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:2,paginationItems:[{id:"90",title:"Human Development",coverUrl:"https://cdn.intechopen.com/series_topics/covers/90.jpg",editor:{id:"191040",title:"Dr.",name:"Tal",middleName:null,surname:"Dotan Ben-Soussan",slug:"tal-dotan-ben-soussan",fullName:"Tal Dotan Ben-Soussan",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSBf1QAG/Profile_Picture_2022-03-18T07:56:11.jpg",biography:"Tal Dotan Ben-Soussan, Ph.D., is the director of the Research Institute for Neuroscience, Education and Didactics (RINED) – Paoletti Foundation. Ben-Soussan leads international studies on training and neuroplasticity from neurophysiological and psychobiological perspectives. As a neuroscientist and bio-psychologist, she has published numerous articles on neuroplasticity, movement and meditation. She acts as an editor and reviewer in several renowned journals and coordinates international conferences integrating theoretical, methodological and practical approaches on various topics, such as silence, logics and neuro-education. She lives in Assisi, Italy.",institutionString:"Research Institute for Neuroscience, Education and Didactics, Patrizio Paoletti Foundation",institution:null},editorTwo:null,editorThree:null,editorialBoard:[{id:"337845",title:"Prof.",name:"Anke",middleName:null,surname:"Koenig",slug:"anke-koenig",fullName:"Anke Koenig",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000032KEmKQAW/Profile_Picture_2022-03-28T08:12:49.jpg",institutionString:null,institution:{name:"University of Vechta",institutionURL:null,country:{name:"Germany"}}},{id:"28286",title:"Dr.",name:"Fernanda Dreux Miranda",middleName:null,surname:"Fernandes",slug:"fernanda-dreux-miranda-fernandes",fullName:"Fernanda Dreux Miranda Fernandes",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYOLpQAO/Profile_Picture_1643350340880",institutionString:null,institution:{name:"University of Sao Paulo",institutionURL:null,country:{name:"Brazil"}}},{id:"289526",title:"Dr.",name:"Michael John",middleName:null,surname:"Stones",slug:"michael-john-stones",fullName:"Michael John Stones",profilePictureURL:"https://mts.intechopen.com/storage/users/289526/images/system/289526.png",institutionString:null,institution:{name:"Lakehead University",institutionURL:null,country:{name:"Canada"}}}]}]},overviewPageOFChapters:{paginationCount:23,paginationItems:[{id:"82392",title:"Nanomaterials as Novel Biomarkers for Cancer Nanotheranostics: State of the Art",doi:"10.5772/intechopen.105700",signatures:"Hao Yu, Zhihai Han, Cunrong Chen and Leisheng Zhang",slug:"nanomaterials-as-novel-biomarkers-for-cancer-nanotheranostics-state-of-the-art",totalDownloads:22,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering - Annual Volume 2022",coverURL:"https://cdn.intechopen.com/books/images_new/11405.jpg",subseries:{id:"9",title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering"}}},{id:"82184",title:"Biological Sensing Using Infrared SPR Devices Based on ZnO",doi:"10.5772/intechopen.104562",signatures:"Hiroaki Matsui",slug:"biological-sensing-using-infrared-spr-devices-based-on-zno",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:[{name:"Hiroaki",surname:"Matsui"}],book:{title:"Biosignal Processing",coverURL:"https://cdn.intechopen.com/books/images_new/11153.jpg",subseries:{id:"7",title:"Bioinformatics and Medical Informatics"}}},{id:"82122",title:"Recent Advances in Biosensing in Tissue Engineering and Regenerative Medicine",doi:"10.5772/intechopen.104922",signatures:"Alma T. Banigo, Chigozie A. Nnadiekwe and Emmanuel M. 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For 20 years, he has studied the analysis and processing of biomedical images, emphasizing the full automation of measurement for a large inter-individual variability of patients. Dr. Koprowski has authored more than a hundred research papers with dozens in impact factor (IF) journals and has authored or co-authored six books. Additionally, he is the author of several national and international patents in the field of biomedical devices and imaging. Since 2011, he has been a reviewer of grants and projects (including EU projects) in biomedical engineering.",institutionString:null,institution:{name:"University of Silesia",institutionURL:null,country:{name:"Poland"}}}]},{type:"book",id:"7218",title:"OCT",subtitle:"Applications in Ophthalmology",coverURL:"https://cdn.intechopen.com/books/images_new/7218.jpg",slug:"oct-applications-in-ophthalmology",publishedDate:"September 19th 2018",editedByType:"Edited by",bookSignature:"Michele Lanza",hash:"e3a3430cdfd6999caccac933e4613885",volumeInSeries:2,fullTitle:"OCT - Applications in Ophthalmology",editors:[{id:"240088",title:"Prof.",name:"Michele",middleName:null,surname:"Lanza",slug:"michele-lanza",fullName:"Michele Lanza",profilePictureURL:"https://mts.intechopen.com/storage/users/240088/images/system/240088.png",biography:"Michele Lanza is Associate Professor of Ophthalmology at Università della Campania, Luigi Vanvitelli, Napoli, Italy. His fields of interest are anterior segment disease, keratoconus, glaucoma, corneal dystrophies, and cataracts. His research topics include\nintraocular lens power calculation, eye modification induced by refractive surgery, glaucoma progression, and validation of new diagnostic devices in ophthalmology. \nHe has published more than 100 papers in international and Italian scientific journals, more than 60 in journals with impact factors, and chapters in international and Italian books. He has also edited two international books and authored more than 150 communications or posters for the most important international and Italian ophthalmology conferences.",institutionString:'University of Campania "Luigi Vanvitelli"',institution:{name:'University of Campania "Luigi Vanvitelli"',institutionURL:null,country:{name:"Italy"}}}]},{type:"book",id:"7560",title:"Non-Invasive Diagnostic Methods",subtitle:"Image Processing",coverURL:"https://cdn.intechopen.com/books/images_new/7560.jpg",slug:"non-invasive-diagnostic-methods-image-processing",publishedDate:"December 19th 2018",editedByType:"Edited by",bookSignature:"Mariusz Marzec and Robert Koprowski",hash:"d92fd8cf5a90a47f2b8a310837a5600e",volumeInSeries:3,fullTitle:"Non-Invasive Diagnostic Methods - Image Processing",editors:[{id:"253468",title:"Dr.",name:"Mariusz",middleName:null,surname:"Marzec",slug:"mariusz-marzec",fullName:"Mariusz Marzec",profilePictureURL:"https://mts.intechopen.com/storage/users/253468/images/system/253468.png",biography:"An assistant professor at Department of Biomedical Computer Systems, at Institute of Computer Science, Silesian University in Katowice. Scientific interests: computer analysis and processing of images, biomedical images, databases and programming languages. 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Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},subseries:[{id:"14",title:"Cell and Molecular Biology",keywords:"Omics (Transcriptomics; Proteomics; Metabolomics), Molecular Biology, Cell Biology, Signal Transduction and Regulation, Cell Growth and Differentiation, Apoptosis, Necroptosis, Ferroptosis, Autophagy, Cell Cycle, Macromolecules and Complexes, Gene Expression",scope:"The Cell and Molecular Biology topic within the IntechOpen Biochemistry Series aims to rapidly publish contributions on all aspects of cell and molecular biology, including aspects related to biochemical and genetic research (not only in humans but all living beings). We encourage the submission of manuscripts that provide novel and mechanistic insights that report significant advances in the fields. Topics include, but are not limited to: Advanced techniques of cellular and molecular biology (Molecular methodologies, imaging techniques, and bioinformatics); Biological activities at the molecular level; Biological processes of cell functions, cell division, senescence, maintenance, and cell death; Biomolecules interactions; Cancer; Cell biology; Chemical biology; Computational biology; Cytochemistry; Developmental biology; Disease mechanisms and therapeutics; DNA, and RNA metabolism; Gene functions, genetics, and genomics; Genetics; Immunology; Medical microbiology; Molecular biology; Molecular genetics; Molecular processes of cell and organelle dynamics; Neuroscience; Protein biosynthesis, degradation, and functions; Regulation of molecular interactions in a cell; Signalling networks and system biology; Structural biology; Virology and microbiology.",annualVolume:11410,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"79367",title:"Dr.",name:"Ana Isabel",middleName:null,surname:"Flores",fullName:"Ana Isabel Flores",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRpIOQA0/Profile_Picture_1632418099564",institutionString:null,institution:{name:"Hospital Universitario 12 De Octubre",institutionURL:null,country:{name:"Spain"}}},{id:"328234",title:"Ph.D.",name:"Christian",middleName:null,surname:"Palavecino",fullName:"Christian Palavecino",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000030DhEhQAK/Profile_Picture_1628835318625",institutionString:null,institution:{name:"Central University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"186585",title:"Dr.",name:"Francisco Javier",middleName:null,surname:"Martin-Romero",fullName:"Francisco Javier Martin-Romero",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB3HQAW/Profile_Picture_1631258137641",institutionString:null,institution:{name:"University of Extremadura",institutionURL:null,country:{name:"Spain"}}}]},{id:"15",title:"Chemical Biology",keywords:"Phenolic Compounds, Essential Oils, Modification of Biomolecules, Glycobiology, Combinatorial Chemistry, Therapeutic peptides, Enzyme Inhibitors",scope:"Chemical biology spans the fields of chemistry and biology involving the application of biological and chemical molecules and techniques. In recent years, the application of chemistry to biological molecules has gained significant interest in medicinal and pharmacological studies. This topic will be devoted to understanding the interplay between biomolecules and chemical compounds, their structure and function, and their potential applications in related fields. Being a part of the biochemistry discipline, the ideas and concepts that have emerged from Chemical Biology have affected other related areas. This topic will closely deal with all emerging trends in this discipline.",annualVolume:11411,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",institutionString:null,institution:{name:"Anadolu University",institutionURL:null,country:{name:"Turkey"}}},editorTwo:{id:"13652",title:"Prof.",name:"Deniz",middleName:null,surname:"Ekinci",fullName:"Deniz Ekinci",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYLT1QAO/Profile_Picture_1634557223079",institutionString:null,institution:{name:"Ondokuz Mayıs University",institutionURL:null,country:{name:"Turkey"}}},editorThree:null,editorialBoard:[{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",fullName:"Abdulsamed Kükürt",profilePictureURL:"https://mts.intechopen.com/storage/users/219081/images/system/219081.png",institutionString:null,institution:{name:"Kafkas University",institutionURL:null,country:{name:"Turkey"}}},{id:"241413",title:"Dr.",name:"Azhar",middleName:null,surname:"Rasul",fullName:"Azhar Rasul",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRT1oQAG/Profile_Picture_1635251978933",institutionString:null,institution:{name:"Government College University, Faisalabad",institutionURL:null,country:{name:"Pakistan"}}},{id:"178316",title:"Ph.D.",name:"Sergey",middleName:null,surname:"Sedykh",fullName:"Sergey Sedykh",profilePictureURL:"https://mts.intechopen.com/storage/users/178316/images/system/178316.jfif",institutionString:null,institution:{name:"Novosibirsk State University",institutionURL:null,country:{name:"Russia"}}}]},{id:"17",title:"Metabolism",keywords:"Biomolecules Metabolism, Energy Metabolism, Metabolic Pathways, Key Metabolic Enzymes, Metabolic Adaptation",scope:"Metabolism is frequently defined in biochemistry textbooks as the overall process that allows living systems to acquire and use the free energy they need for their vital functions or the chemical processes that occur within a living organism to maintain life. Behind these definitions are hidden all the aspects of normal and pathological functioning of all processes that the topic ‘Metabolism’ will cover within the Biochemistry Series. Thus all studies on metabolism will be considered for publication.",annualVolume:11413,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",institutionString:null,institution:{name:"Artois University",institutionURL:null,country:{name:"France"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"243049",title:"Dr.",name:"Anca",middleName:null,surname:"Pantea Stoian",fullName:"Anca Pantea Stoian",profilePictureURL:"https://mts.intechopen.com/storage/users/243049/images/system/243049.jpg",institutionString:null,institution:{name:"Carol Davila University of Medicine and Pharmacy",institutionURL:null,country:{name:"Romania"}}},{id:"203824",title:"Dr.",name:"Attilio",middleName:null,surname:"Rigotti",fullName:"Attilio Rigotti",profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institutionString:null,institution:{name:"Pontifical Catholic University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"300470",title:"Dr.",name:"Yanfei (Jacob)",middleName:null,surname:"Qi",fullName:"Yanfei (Jacob) Qi",profilePictureURL:"https://mts.intechopen.com/storage/users/300470/images/system/300470.jpg",institutionString:null,institution:{name:"Centenary Institute of Cancer Medicine and Cell Biology",institutionURL:null,country:{name:"Australia"}}}]},{id:"18",title:"Proteomics",keywords:"Mono- and Two-Dimensional Gel Electrophoresis (1-and 2-DE), Liquid Chromatography (LC), Mass Spectrometry/Tandem Mass Spectrometry (MS; MS/MS), Proteins",scope:"With the recognition that the human genome cannot provide answers to the etiology of a disorder, changes in the proteins expressed by a genome became a focus in research. Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. Currently, proteomics relies mainly on mass spectrometry (MS) combined with electrophoretic (1 or 2-DE-MS) and/or chromatographic techniques (LC-MS/MS). MS is an excellent tool that has gained popularity in proteomics because of its ability to gather a complex body of information such as cataloging protein expression, identifying protein modification sites, and defining protein interactions. The Proteomics topic aims to attract contributions on all aspects of MS-based proteomics that, by pushing the boundaries of MS capabilities, may address biological problems that have not been resolved yet.",annualVolume:11414,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null,editorialBoard:[{id:"72288",title:"Dr.",name:"Arli Aditya",middleName:null,surname:"Parikesit",fullName:"Arli Aditya Parikesit",profilePictureURL:"https://mts.intechopen.com/storage/users/72288/images/system/72288.jpg",institutionString:null,institution:{name:"Indonesia International Institute for Life Sciences",institutionURL:null,country:{name:"Indonesia"}}},{id:"40928",title:"Dr.",name:"Cesar",middleName:null,surname:"Lopez-Camarillo",fullName:"Cesar Lopez-Camarillo",profilePictureURL:"https://mts.intechopen.com/storage/users/40928/images/3884_n.png",institutionString:null,institution:{name:"Universidad Autónoma de la Ciudad de México",institutionURL:null,country:{name:"Mexico"}}},{id:"81926",title:"Dr.",name:"Shymaa",middleName:null,surname:"Enany",fullName:"Shymaa Enany",profilePictureURL:"https://mts.intechopen.com/storage/users/81926/images/system/81926.png",institutionString:"Suez Canal University",institution:{name:"Suez Canal University",institutionURL:null,country:{name:"Egypt"}}}]}]}},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"chapter.detail",path:"/chapters/63478",hash:"",query:{},params:{id:"63478"},fullPath:"/chapters/63478",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()