Atherosclerosis is a chronic inflammatory process that initiates with accumulation of apolipoprotein B containing lipoproteins (LPs) in the subendothelium (intima), especially in areas where the laminar flow is disturbed. LP retention triggers an inflammatory response leading to activation of endothelial and vascular smooth muscle cells that culminates with recruitment of leukocytes. Atherosclerosis is the leading cause of vascular disease worldwide being its major clinical manifestations ischemic heart disease, ischemic stroke, and peripheral arterial disease. Even though a lot has been done to unravel the role of turbulent flow and mechanotransduction for atherosclerosis development, little is known about the role of plasma membrane (PM) cholesterol in this process. This chapter is going to be focused on exploring what has been done so far to decipher the role of PM cholesterol in regulating actin architecture, cellular mechanical properties, and cellular contractility in muscle and nonmuscle cells.
The role that cholesterol plays in cardiovascular diseases is widely known and studied . However, less appreciated is the importance that cholesterol has in orchestrating other important cellular functions, such as cellular contractility and cytoskeleton organization. Cellular contractility is the ability of a cell to exert mechanical work on a substrate or on a neighboring cell due to actomyosin cytoskeleton enzymatic activity . In muscle and nonmuscle cells, most of the cholesterol content is localized at the plasma membrane  where it can partition into microdomains called lipid rafts. Lipid rafts are highly dynamic regions of the plasma membrane that contain sphingolipids and cholesterol and are responsible for compartmentalizing and regulating several intracellular signaling events [4, 5, 6]. One way of studying the importance of cholesterol for a specific cellular function is to decrease its concentration by either interfering directly with its synthesis, through the mevalonate pathway, or by chelating the molecule directly through the use of cyclodextrins . However, depending on how one does the cholesterol depletion, the effects on cellular contractility can be opposite. Differences in muscle versus nonmuscle cell contractile behavior are observed upon cholesterol depletion using cyclodextrins. Muscle cells get impairment in their contractile machinery  whereas nonmuscle cells get more contractile . This book chapter gives an overview about how cholesterol is organized at the plasma membrane and how its depletion changes cellular contractile properties.
2. Lipid rafts and membrane heterogeneity
Even though the Cell Theory started to be developed in the nineteenth century , it was not until the first quarter of the twentieth century that the idea of a membrane encompassing the cell was experimentally observed. In 1924, the Dutch physiologists Gorter and Grendel elegantly demonstrated, for the first time, the existence of a lipid bilayer surrounding red blood cells of various animals . They isolated erythrocytes from humans and different mammals (rabbit, dog, guinea pig, sheep, and goat), extracted their lipids using acetone, and let those lipids spread on an air-water interface of a Langmuir-Adam apparatus. By knowing the number and area of the erythrocytes used in their experiment, they concluded that those cells were surrounded by a layer of lipids whose thickness was equivalent to two molecules , hence a lipid bilayer. The Gorter and Grendel model for cellular plasma membrane considered only the lipid nature of this cellular component and, because of that and due to other experimental and theoretical inaccuracies, it failed in explaining satisfactorily experimental results for membrane thickness , membrane tension , membrane electrical capacitance , and membrane permeability .
In order to explain those membrane properties, another model, called the paucimolecular model, was proposed by Danielli and coworkers in 1935 [12, 16, 17]. By examining the surface tension of a single drop of mackerel egg oil, Danielle and Harvey found that the value they measured was lower than the equivalent obtained for nonliving pure water-oil systems. They hypothesized that the difference observed for surface tension in living versus nonliving water-oil systems was due to the fact that the plasma membrane not only contained lipids but also proteins adsorbed in the lipid bilayer . In the same year, Danielli and Davson  extended the paucimolecular model in order to explain permeability experiments. In that model, the layer of proteins adsorbed on top of a lipid film was able to discern size of molecules and charge of ions that were penetrating the membrane. This lipid film containing adsorbed proteins was considered to be relatively stable with mosaics consisting of practically impenetrable regions and hydrated areas where anions could move through .
For the next 30 years, the paucimolecular model was the most accepted one among the scientific community. However, with the advancement of microscopy techniques and structural studies, a new and more robust model, named the fluid mosaic model, was proposed in 1972 by Singer and Nicolson . According to that model, integral transmembrane proteins are arranged in the plasma membrane of living cells such that the polar regions are facing the aqueous phase and the hydrophobic regions are embedded on a viscous phospholipid bilayer and those proteins are able to move freely on that two-dimensional, approximately homogeneous fluid “sea” of phospholipids . One year later, in 1973, Bretscher published a Science paper in which he discusses overall membrane organization based on evidences collected from experiments performed in red blood cells . According to that paper, the plasma membrane of mammalian cells was not as simple as depicted by the fluid mosaic model. Some of the integral proteins span the membrane and their glycosylation is responsible for locking them at the membrane impeding their migration to the cytoplasm. Another important contribution from this paper is that proteins not only interact with the outer layer of the plasma membrane but also with the inner layer, and membrane proteins are a subtype of cytoplasmic proteins that are not secreted . In the same year, Yu and collaborators, also performing experiments in red blood cells, showed that when those cells are incubated with the nonionic detergent Triton X-100, there are some fractions of the cellular proteins that are resistant to the detergent extraction and seem to form oligomeric complexes with some of the lipid components, which were preferentially composed by nonglycosylated proteins and sphingolipids .
2.1. Lipid rafts
The idea of possible membrane microdomains started to be speculated in the early 1970s [20, 21] and experimentally demonstrated in 1982 by Karnovsky , who showed that there were multiple phases in the lipid environment of a membrane. One type of microdomain can be formed by cholesterol and sphingolipids . These microdomains were already shown to be present in cell membranes . In 1988, after several experimental demonstrations, Simons and van Meer called these microdomains as lipid rafts . Thus, lipid rafts are defined as small, heterogeneous, and highly dynamic microdomains enriched in cholesterol, glycosphingolipids, and proteins that are much more organized than the surrounding lipid bilayer . These membrane microdomains serve as organizing clusters capable of influencing several cellular processes such as membrane trafficking and neurotransmission .
The most striking difference between lipid rafts and the plasma membrane from which they are derived from is the lipid composition. Experiments have shown that rafts contain much more cholesterol than the surrounding bilayer [27, 28]. Cholesterol, therefore, works as a sort of “dynamic glue” that maintains the raft together , serving as a molecular spacer and filling the empty spaces between sphingolipids . One of the main challenges when studying lipid rafts in living cells is their size. They are small microdomains ranging from 10 to 200 nm, below the classical diffraction limit of the optical microscope . The first studies in the field considered methods to extract and separate rafts from the surrounding membrane. The procedure would take advantage of lipid raft resistance to nonionic detergents. When detergents are added to cells, the fluid membrane will dissolve while the lipid rafts may remain intact and could be extracted ; however, the validity of this methodology has been called into question due to ambiguities in the lipids and proteins obtained after extraction . Other methods, based on synthetic membranes, were also used, however with many drawbacks. Firstly, synthetic membranes either lack or have lower protein concentration when compared to cell membranes . Secondly, it is very difficult to simulate, in synthetic membranes, the membrane-cytoskeletal interactions that occur in cell membranes, although some recent studies have been able to overcome these limitations [33, 34, 35]. Finally, another problem includes the lack of natural asymmetry between the bilayer leaflets .
Although lipid rafts present sizes below the classical diffraction limit of the optical microscope, fluorescence microscopy has been extensively used in the field. For example, fluorophores conjugated to cholera-toxin B-subunit, which binds to the raft constituent ganglioside GM1, is used. Also, lipophilic membrane dyes (such as Laudran) that either partition between rafts and the surrounding membrane or change their fluorescent properties in response to membrane phase are used. Finally, lipid rafts can also be fluorescently labeled in cells after genetic expression of fluorescent fusion proteins .
Another methodology, which has been widely used in the study of lipid rafts, is the manipulation of cholesterol contents in membranes. Sequestration (using filipin, nystatin, or amphotericin), depletion and removal (using methyl-β-cyclodextrin, MβCD), or inhibition of cholesterol synthesis (using 3-hydroxy-3-methyl-glutaryl-coenzyme A, HMG-CoA, reductase inhibitors) are great examples of how cholesterol can be manipulated in lipid raft studies . Several questions, however, have been raised against the effectiveness of the experimental design when disrupting lipid rafts. Acute methods of cholesterol depletion, which disrupt the rafts, can also disrupt another lipid, called PI(4,5)P2, which plays an important role in cytoskeletal regulation . Thus, the loss of a particular cellular function after cholesterol depletion cannot necessarily be attributed only to raft disruption, since other processes are also being affected.
Despite these limitations, more sophisticated methods have been applied in order to fight against the problems of small size and dynamic nature of lipid rafts. These methods include single particle and molecule tracking using very sensitive CCD cameras together with total internal reflection microscopy. These combined techniques provide information of the diffusion coefficient of particles in the membrane and also reveal membrane corrals, barriers, and sites of confinement . Finally, other optical techniques have been used to elucidate other features of lipid rafts: fluorescence correlation spectroscopy, to gain information of fluorophore mobility in the membrane ; fluorescence resonance energy transfer, to detect when fluorophores are in close proximity , and optical tweezers, to give information about the membrane mechanical parameters [8, 41]. In the future, it is expected that other super-resolution microscopy techniques, such as stimulated emission depletion microscopy  or various forms of structured illumination microscopy may overcome the problems imposed by the diffraction limit.
Apart from the different imaging methods, research over the last decades have demonstrated the existence of two types of rafts: (1) planar lipid rafts (also known as noncaveolar or glycolipid rafts) and caveolae. Planar rafts are known to be continuous with the plane of the plasma membrane (not invaginated) and contain flotillin proteins. Caveolae are flask shaped invaginations of the plasma membrane that contain caveolin proteins. Both types are enriched in cholesterol and sphingolipids. Flotillin and caveolins can either recruit or separate other molecules from lipid rafts and caveolae, respectively, thus playing an essential role in signal transduction .
Caveolae are plasma membrane invaginations with a diameter ranging from 60 to 80 nm and were first identified in the early 1950s by electron microscopy . These invaginations are expressed in various cell types such as smooth muscle, fibroblasts, endothelial cells and adipocytes, among several others. Their functions are diverse and include endocytosis, calcium signaling as well as regulation of various cell signaling pathways .
The major constituent of caveolae is caveolin1 , followed by two other isoforms: caveolin2  and the muscle-specific caveolin3 . All three caveolin proteins share a common topology with both their N and C terminal domains in the cytoplasm and a long hairpin transmembrane domain. All three types of caveolin are formed inside the cells, more precisely in the Golgi apparatus, as monomers . However, as soon as they enter in the secretory pathway, they start to be structured as oligomers . For caveolin1, for example, its exit from the Golgi apparatus is accelerated upon addition of cholesterol . The oligomerization ability of caveolin1 is crucial for caveolae formation . Caveolin2 has also been implicated in caveolae formation , and although caveolin1 null mouse shows a significant decrease in caveolae assembly, they are still present in the caveolin2 mouse . In muscle, caveolin3 is crucial for caveolae formation. Mutations or loss of caveolin3 result in dystrophic phenotypes [54, 55].
Caveolin expression at the plasma membrane is not the only inducer of caveolae formation. Cholesterol extraction has been extensively shown to disrupt caveolae at the plasma membrane  since it is required for caveolin incorporation into raft domains at the plasma membrane, a critical event for caveolae formation .
Although caveolins and cholesterol were initially thought to be necessary and sufficient for caveolae formation, several studies have shown additional molecular players called cavins. This protein family has four different members already described: cavin-1 (also called PTRF) , cavin-2 (also called SDPR) , cavin-3 (also called SRBC) , and the muscle-specific cavin-4 (also called MURC) . These four proteins are essential to caveolae formation and functions. Thus, caveolae formation is a highly complex and regulated cellular process. It has been estimated that ~150–200 caveolin monomers are necessary to associate with ~50–60 cavins in order to form a single caveola [61, 62]. Moreover, caveolae architecture was recently proposed to be a dodecahedron formed by cavins aligned with their vertices and also in the caveolin oligomers located at each of the pentagonal faces [61, 62].
As already mentioned, caveolae represent a subdomain of lipid rafts . Confocal microscopy has shown that the distribution of GM1, a well characterized raft marker, do not merge with caveolin1 . Another raft marker, flotillin, defines noncaveolar rafts and merges with GM1 . Thus, rafts exhibit a heterogeneous distribution over the plasma membrane changing between caveolar (invaginated) and noncaveolar (planar) regions.
3. Actomyosin cytoskeleton: the contractile machinery of muscle and nonmuscle cells
The cytoskeleton constitutes a dynamic network of filaments that exists in the inner space of a cell. This network not only provides scaffolding but is also responsible for transporting organelles, generating and transducting mechanical forces. The cytoskeleton maintains cellular organization by linking together several cellular components in such a way that it mediates communication across the entire cell and, therefore, has a tremendous impact on cellular functions . Three main filaments constitute the cytoskeleton, each one with its distinct protein composition and function: the microtubules, intermediate filaments, and microfilaments.
Microfilaments, also known as actin filaments, are ~7 nm in width. They are primarily composed of actin, the most abundant protein in cells. Actin filaments can create a huge number of arrays, such as bundles, two-dimensional networks, and three-dimensional gels. These different structural organizations are controlled by several actin-binding proteins and are found, for example, at the leading edge of a moving cell, particularly in filopodia and lamellipodia (Figure 1B), which causes the actin filaments to be the primary cytoskeletal component to drive cell motility . Actin filaments also allow the cell to probe or sense its microenvironment. More stable networks of actin filaments, known as stress fibers, allow cells to brace against the underlying surface . Thus, microfilaments can either be alone, as simple filaments, or together with the myosin filaments, which are part of the actomyosin contractile apparatus, in muscle and nonmuscle cells. Myosin filaments, associated with actin filaments, use ATP hydrolysis to exert forces against stress fibers during cytoskeletal contractility .
The actomyosin contractile machinery is relatively well conserved, despite some differences in organization and dynamics among different cell types. Actin filaments are polarized as barbed ends (fast-growing end) and pointed ends (slow-growing ends) and serve as scaffold for myosin filaments, which vary in size ranging from a few heads, in nonmuscle cells, to hundreds of heads in thick filaments of striated muscle cells. The myosin filaments drive the translocation of actin filaments toward their barbed ends. This event can trigger either the contraction or extension of two actin filaments .
The most well understood example of a contractile actomyosin apparatus is found in striated muscles and is called sarcomere. The sarcomeres are what give the striated muscles their appearance. It is known that a sarcomere is defined as a segment between two neighboring Z-lines. The Z-lines (from the German “Zwischenscheibe”) are “dark” lines that appear in both extremities of a sarcomere (Figure 1A). They act as anchoring points for actin filaments. Surrounding two Z-lines, there are two regions called I-bands, regions of actin filaments that are not superimposed by myosin filaments (Figure 1A). Between the two I-bands is an A-band, which contains the entire length of myosin filaments and part of actin filaments that extend from I-bands (Figure 1A). The barbed ends of actin filaments are localized at the Z-line. The myosin filaments are segregated toward the pointed ends of actin filaments. Several other proteins are present and allow the stability of a sarcomere. The interaction between actin and myosin filaments in the A-band of a sarcomere is responsible for muscle contraction (Figure 1A) .
The contraction starts when a motor neuron releases acetylcholine, a neurotransmitter that binds to a postsynaptic nicotinic acetylcholine receptor on the muscle fiber, causing a change in receptor conformation and allowing an influx of sodium ions followed by postsynaptic action potential initiation. The action potential travels through T-tubules until it reaches the sarcoplasmic reticulum, where it activates voltage-gated L-type calcium channels. The initial inward flow of calcium from the L-type calcium channels activates ryanodine receptors, which releases a huge amount of calcium ions from the sarcoplasmic reticulum toward the cytoplasm of muscle cells. This mechanism is called calcium-induced calcium-release . Inside muscle cells, the protein tropomyosin covers the myosin binding sites of actin filaments in the sarcomere. In order to allow contraction, tropomyosin must be moved from its original place. Initially, tropomyosin is attached to the actin filaments, covering myosin binding sites. When calcium ions enter in the muscle cell cytoplasm, they immediately bind to troponin-C and trigger a change in the structure of tropomyosin. This change in conformation forces tropomyosin to reveal the myosin-binding sites on actin filaments and allows myosin filaments to pull antiparallel actin filaments together. Muscle contraction ends when calcium ions are pumped back from the muscle cell cytoplasm into the sarcoplasmic reticulum, allowing the contractile machinery to relax .
The actomyosin cytoskeleton in nonmuscle and smooth muscle cells is organized in similar ways, both different from sarcomeres of striated muscles. Nonmuscle and smooth muscle cells use myosin to generate contractility during migration, cytokinesis, as well as cell-cell and cell-matrix junctions, for example . Nonaligned actomyosin networks, with actin filaments and clusters of bipolar myosin filaments interacting with each other at their ends, represent the simplest contractile machinery in nonmuscle and smooth muscle cells, especially in cytoskeletal regions that do not have stress fibers [67, 68]. Smooth muscle and nonmuscle cells also contain more organized actomyosin bundles, such as transverse arcs, radial stress fibers, peripheral bundles, and ventral stress fibers (Figure 1B) . Transverse stress fibers or arcs (Figure 1B) are formed after reorganization of lamellipodial actin filaments  during lamellipodium retraction . This process is driven by myosin filaments, which become co-aligned with actin filaments and form stacks separated by alfa-actinin . Radial stress fibers (Figure 1B), on the other hand, are anchored to focal adhesions in one end. Myosin molecules are recruited to the tips of focal adhesions, where nascent radial stress fibers start to form . Contractility of the radial-transverse-stress fiber network leads to the formation of ventral stress fibers (Figure 1B) attached to focal adhesions on both ends . Also, actomyosin filaments from nonmuscle and smooth muscle cells are highly dynamic when compared to striated muscle cells. Both actin and myosin can frequently undergo turnover or cycles of assembly/disassembly [73, 74].
Based on all above-described features, the most striking differences between stress fibers and sarcomeres are: (1) the molecular composition is cell-specific , (2) stress fiber contraction is regulated by phosphorylation of myosin light chain (pMLC) (see pMLC regulation in Section 4), while sarcomere contraction is regulated by troponin switching , (3) stress fibers are approximately one order of magnitude thinner, less organized (with different directions and lengths) and with less coordinated contraction when compared to sarcomeres , (4) the magnitude of the force they produce is different, while the stress fiber contractile forces come from individual cells and are applied, through focal adhesions, to the extracellular environment in which these cells are located , the sarcomeres from striated muscles can transmit contractile forces over macroscopic lengths. Finally, (5) striated muscles can rapidly contract and relax based on action potentials and Ca2+ release [64, 78] while stress fibers from smooth muscle and nonmuscle cells respond much slower and do not depend solely on electrical pulses .
While striated muscle cells present different actomyosin organization and features when compared to nonmuscle and smooth muscle cells, these three cell types share a common actomyosin structure: the actin cortex, also known as cell cortex or actomyosin cortex. This is a thin and highly disordered contractile actomyosin network underling the plasma membrane of cells . It was first discovered in large cells, like amoeba and animal eggs and subsequently, extrapolated to all animal cells . Non-adherent cells , cells during mitosis , or cells performing amoeboid-like migration  present a well distributed and uniform actin cortex. Cells spread over flat surfaces, although more difficult to be observed, also present a cortical layer of actomyosin, as shown by electron microscopy . The actomyosin appears as an isotropic network parallel (and some perpendicular) to the plasma membrane with a width of 20–250 nm [83, 84]. Numerous actin-binding proteins have already been described to be part of the actin cortex , most of them are classical actin-binding proteins; however, little is known about how actin cortex is assembled.
The actin cortex plays a major role in cell mechanics as the main determinant of cell surface tension . Biophysical methods like micropipette aspiration and membrane tether pulling assays (using either optical tweezers or atomic force microscopy) have been used to measure cell surface mechanics . Micropipette aspiration is a suitable technique to measure the overall cellular tension, which is a combination of the tension in the plasma membrane together with the tension in the underlying actin cytoskeleton . Moreover, membrane tether pulling assays also bring information about the membrane itself and its attachment with the cortical cytoskeleton [86, 87]. During bleb formation, for example, a momentary separation between the plasma membrane and the actin cortex occurs .
Biophysical methods show that the membrane-cortex attachment is the major determinant in cell surface tension [89, 90] and that different cells have different surface tension values, indicating that there may have different mechanisms to maintain surface tension homeostasis among cells . Modifications of specific cross-linking proteins, whose function is to link the plasma membrane to the actin cortex, can induce changes in cell surface tension [91, 92, 93, 94]. Also, actin filament disruption or myosin inhibition can reduce cell surface tension [90, 95, 96, 97]. Changes in membrane composition, particularly in cholesterol content, have also been shown to influence cell surface tension. MβCD causes an increase in tension in embryonic kidney cells , fibroblasts  and cardiomyocytes . This increase is not only due to changes in membrane composition, but it also affects the actomyosin cytoskeleton. In fibroblasts, MβCD treatment shows an increase in stress fiber formation  whereas cardiomyocytes show sarcomeric disorganization together with contraction abnormalities .
4. Cellular contractility in nonmuscle cells: the role of Rho and pleiotropic effects of statins
In order to divide, migrate, and undergo tissue morphogenesis, cells change shape and exert forces either on the substrate that they are attached to or on the neighboring cells. Nonmuscle cells generate contractile stresses via molecular motors, such as myosin, that are able to convert chemical energy into mechanical work . Myosin activity is controlled through phosphorylation of its light chain via myosin light chain kinase (MLCK) [100, 101] which, in turn, is activated by Rho kinase ROCK and the small GTPase Rho A upstream. In 1985, the Rho gene was first isolated, from abdominal ganglia of the Aplysia, and identified as a member of the Ras family . In 1990, after injecting a constitutively active form of Rho (Vall4rho), Paterson and collaborators verified that active Rho is able to cause changes in cellular morphology inducing formation of stress fibers . In 1992, Ridley and Hall showed that active RhoA induces formation of stress fibers and focal adhesions upon growth factor stimulation . Being a GTPase, Rho can switch back and forth between its active state, when bound to GTP, and its inactive state, when bound to GDP. The switching process is finely regulated by guanine nucleotide exchange factors (GEFs), which promote activation, and GTPase-activating proteins (GAPs), which promote inactivation. There are approximately 60 GEFs and 70 GAPs that were already identified in the human genome . In order to be activated, Rho goes through some posttranslational modifications that are essential to induce Rho migration toward the plasma membrane, where it gets activated. Prenylation is a posttranslational modification that is known to be pivotal for Rho translocation to the membrane . Protein prenylation is essentially an insertion of a prenyl group, which is a hydrophobic group, to the c-terminal of a protein. That way, the protein has a lipid anchor that allows it to stay membrane bound. There are two types of prenylation: farnesylation and geranyl-geranylation, which are regulated by farnesyltransferase and geranylgeranyltransferase I, respectively . In the case of RhoA, the protein gets geranyl-geranylated before it goes to the membrane to get activated and trigger downstream effectors [108, 109] (Figure 2).
Both isoprenoids, farnesyl pyrophosphate and geranylgeranyl pyrophosphate, are synthetized by the mevalonate pathway. Interestingly, cholesterol is the end product of this pathway  (Figure 3). Therefore, by manipulating the mevalonate pathway, one can perturb both cholesterol synthesis and prenylation of important target proteins such as RhoA, and, as a consequence, cellular contractility (Figure 3).
The rate-limiting step of the mevalonate pathway is regulated by the enzyme HMG-CoA reductase. The activity of HMG-CoA reductase is precisely governed by the amount of cholesterol available. There are basically two different sources of cholesterol in the body: the exogenous one (obtained through intestinal absorption of cholesterol from the diet) and the endogenous one (through the
Interestingly, when cholesterol is directly depleted by MβCD, an opposite trend is observed regarding nonmuscle cellular contractility. Human skin fibroblasts, after MβCD treatment, showed a reduction in the mobility of plasma membrane proteins being that reduction in motion a direct result of cytoskeleton reorganization . It was also shown, for bovine aortic endothelial cells, that MβCD-dependent cholesterol depletion increased cortical stiffness  as well as adhesion energy between membrane and cytoskeleton, which decreased the lipid diffusion coefficient . Serum starvation followed by cyclodextrin-mediated cholesterol depletion increased stress fiber formation and RhoA activation in an osteoblast cell line . Later on, those results were also corroborated by our group in a murine fibroblast cell line . Similar features can also be observed in an osteosarcoma cell line U2OS (Figure 4) in which cholesterol depletion led to stress fiber formation and reorganization of actin cytoskeleton. More studies need to be performed in order to understand why different manipulations in cholesterol content trigger opposite results regarding cellular contractility.
5. Cellular contractility in muscle cells: interplay among Ca2+, sarcomeres and cholesterol
Even though statins had been shown to be relatively safe and to promote health benefits to patients with high risks of cardiac diseases, there are some side effects and risks associated with statin therapy. Myotoxicity is one of the most adverse side effects, being the most common clinical outcomes: myosite, myalgia, and rhabdomyolysis . In vitro studies performed on single muscle fibers isolated from rat skeletal muscle showed that fluvastatin and pravastatin led to contractility impairment and vacuolization of the muscle after 72 h of treatment and cell death after 120 h. Those changes in cellular morphology and contraction were proven to be dependent on geranyl-geranylation of GTPases since concomitant incubation of fluvastatin and geranylgeranyl pyrophosphate attenuated the deleterious effects of statins . In vivo and in vitro treatment with simvastatin also led to contractile dysfunction, actin cytoskeleton disruption and apoptosis of smooth muscle cells .
Regarding the effects of direct cholesterol depletion mediated by MβCD on muscle cells, our group demonstrated, using primary cell culture of neonatal rat cardiomyocytes, that a lower cholesterol content increased the contraction rate of those cells and also led to defects in cell relaxation . Moreover, cholesterol depletion increased the Ca2+ cytoplasmic concentration and Ca2+ sparks during contraction. This phenotype can be attributed to changes in caveolin3 and L-type Ca2+ channels distribution across the plasmalemma and hyperactivation of cAMP-dependent PKA activity. Cholesterol-depleted cardiomyocytes also present aberrant myofibrils due to calpain (a Ca2+ sensitive protease) activation. By using high-quality confocal microscopy and quantitative data analysis, this work has set in stone the role of cholesterol in regulating cardiomyocyte contractile behavior . Other groups have also shown, for adult rat cardiomyocytes, that cholesterol depletion due to MβCD incubation changed localization of caveolin-3 from a raft to a nonraft membrane fraction changing MAPK signaling and increasing contractility and intracellular Ca2+ concentration . Adult murine cardiomyocytes treated with MβCD also presented impairment in the T-tubule system and intercalated discs, which reinforces the role of cholesterol in regulating cardiac contractility . More studies need to be performed in order to understand why MβCD-driven cholesterol depletion in nonmuscle cells increase contractile behavior whereas in muscle cells the same treatment tend to abrogate cellular contractility in several levels.
Cholesterol is a very important lipid that controls several cellular processes. This chapter describes how cholesterol is organized in cellular membranes and how it regulates and orchestrates the contractile machinery in muscle and nonmuscle cells. Cholesterol and RhoA protein prenylation share the same synthetic route: the mevalonate pathway. By lowering cholesterol concentration, using either chelating agents, such as MβCD, or inhibitors of HMG-CoA reductase, such as statins, one can observe opposite effects on actin cytoskeleton organization and contractile behavior. Cellular treatments with statins lead to a less-contractile profile, since this drug depletes the amount of prenylated RhoA, which, in turn, is the main upstream regulator of contractility in nonmuscle cells. On the other hand, MβCD-mediated cholesterol depletion induces RhoA activation, stress fiber formation, and increase in cortical stiffness pointing toward a more contractile behavior. In muscle cells, the results are even more intriguing: treatments with either statins or MβCD lead to myofibril disorganization, increase of contraction rate and defects in cell relaxation and in the ability of cells to handle intracellular Ca2+. The reason why muscle and nonmuscle cells behave differently regarding cholesterol depletion is not completely understood and further investigation needs to be performed in order to elucidate this paradigm.
This work was supported by the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ).
Conflict of interest
The authors declare that they have no conflict of interest.
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