Summary of resolution performance of image plane digital holographic methods.
\\n\\n
IntechOpen Book Series will also publish a program of research-driven Thematic Edited Volumes that focus on specific areas and allow for a more in-depth overview of a particular subject.
\\n\\nIntechOpen Book Series will be launching regularly to offer our authors and editors exciting opportunities to publish their research Open Access. We will begin by relaunching some of our existing Book Series in this innovative book format, and will expand in 2022 into rapidly growing research fields that are driving and advancing society.
\\n\\nLaunching 2021
\\n\\nArtificial Intelligence, ISSN 2633-1403
\\n\\nVeterinary Medicine and Science, ISSN 2632-0517
\\n\\nBiochemistry, ISSN 2632-0983
\\n\\nBiomedical Engineering, ISSN 2631-5343
\\n\\nInfectious Diseases, ISSN 2631-6188
\\n\\nPhysiology (Coming Soon)
\\n\\nDentistry (Coming Soon)
\\n\\nWe invite you to explore our IntechOpen Book Series, find the right publishing program for you and reach your desired audience in record time.
\\n\\nNote: Edited in October 2021
\\n"}]',published:!0,mainMedia:{caption:"",originalUrl:"/media/original/132"}},components:[{type:"htmlEditorComponent",content:'With the desire to make book publishing more relevant for the digital age and offer innovative Open Access publishing options, we are thrilled to announce the launch of our new publishing format: IntechOpen Book Series.
\n\nDesigned to cover fast-moving research fields in rapidly expanding areas, our Book Series feature a Topic structure allowing us to present the most relevant sub-disciplines. Book Series are headed by Series Editors, and a team of Topic Editors supported by international Editorial Board members. Topics are always open for submissions, with an Annual Volume published each calendar year.
\n\nAfter a robust peer-review process, accepted works are published quickly, thanks to Online First, ensuring research is made available to the scientific community without delay.
\n\nOur innovative Book Series format brings you:
\n\nIntechOpen Book Series will also publish a program of research-driven Thematic Edited Volumes that focus on specific areas and allow for a more in-depth overview of a particular subject.
\n\nIntechOpen Book Series will be launching regularly to offer our authors and editors exciting opportunities to publish their research Open Access. We will begin by relaunching some of our existing Book Series in this innovative book format, and will expand in 2022 into rapidly growing research fields that are driving and advancing society.
\n\nLaunching 2021
\n\nArtificial Intelligence, ISSN 2633-1403
\n\nVeterinary Medicine and Science, ISSN 2632-0517
\n\nBiochemistry, ISSN 2632-0983
\n\nBiomedical Engineering, ISSN 2631-5343
\n\nInfectious Diseases, ISSN 2631-6188
\n\nPhysiology (Coming Soon)
\n\nDentistry (Coming Soon)
\n\nWe invite you to explore our IntechOpen Book Series, find the right publishing program for you and reach your desired audience in record time.
\n\nNote: Edited in October 2021
\n'}],latestNews:[{slug:"intechopen-supports-asapbio-s-new-initiative-publish-your-reviews-20220729",title:"IntechOpen Supports ASAPbio’s New Initiative Publish Your Reviews"},{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"}]},book:{item:{type:"book",id:"10409",leadTitle:null,fullTitle:"Soil Erosion - Current Challenges and Future Perspectives in a Changing World",title:"Soil Erosion",subtitle:"Current Challenges and Future Perspectives in a Changing World",reviewType:"peer-reviewed",abstract:"Soil erosion is a major environmental issue with a worldwide impact and direct and indirect effects on soil productivity and consequently on human survival. Although a natural process, soil erosion has increased significantly due to human intervention, especially in the last centuries, through diverse activities such as intensive agriculture, overgrazing, urban sprawl, deforestation, and industrial and mining activities. Presently, soil erosion and degradation promoted by human action have reached extreme levels, necessitating urgent measures to promote soil conservation and rehabilitation. This book presents perspectives on soil erosion occurring in different parts of the world as well as some successful initiatives and strategies for soil conservation and rehabilitation.",isbn:"978-1-83962-300-4",printIsbn:"978-1-83962-299-1",pdfIsbn:"978-1-83962-304-2",doi:"10.5772/intechopen.91595",price:119,priceEur:129,priceUsd:155,slug:"soil-erosion-current-challenges-and-future-perspectives-in-a-changing-world",numberOfPages:152,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"100d8afa798aacadca65a149a4f902b5",bookSignature:"António Vieira and Silvio Carlos Rodrigues",publishedDate:"May 12th 2021",coverURL:"https://cdn.intechopen.com/books/images_new/10409.jpg",numberOfDownloads:2749,numberOfWosCitations:0,numberOfCrossrefCitations:2,numberOfCrossrefCitationsByBook:0,numberOfDimensionsCitations:3,numberOfDimensionsCitationsByBook:0,hasAltmetrics:0,numberOfTotalCitations:5,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"June 8th 2020",dateEndSecondStepPublish:"June 29th 2020",dateEndThirdStepPublish:"August 28th 2020",dateEndFourthStepPublish:"November 16th 2020",dateEndFifthStepPublish:"January 15th 2021",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,7",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"103627",title:"Prof.",name:"António",middleName:null,surname:"Vieira",slug:"antonio-vieira",fullName:"António Vieira",profilePictureURL:"https://mts.intechopen.com/storage/users/103627/images/system/103627.png",biography:"Antonio Vieira is a geographer with a Ph.D. in Geography from the University of Coimbra, Portugal (2009). He is an assistant professor in the Department of Geography, University of Minho (UM), Portugal, and an integrated member of the Communication and Society Research Centre (CECS), UM. He is a member of several scientific organizations, including the Portuguese Association of Geomorphologists (Chair from 2017 to 2019) and the Portuguese Association of Risk, Prevention and Security (vice-president since 2015). He is also a member of FuegoRED and coordinator of the FESP International Network. Dr. Vieira’s main research includes granitic geomorphology, geomorphological heritage and changes in land use, GIS and remote sensing, and their application to land use, geomorphological heritage, and soil erosion following forest fires and mitigation measures.",institutionString:"University of Minho",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"1",institution:{name:"University of Minho",institutionURL:null,country:{name:"Portugal"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:{id:"320125",title:"Dr.",name:"Silvio Carlos",middleName:null,surname:"Rodrigues",slug:"silvio-carlos-rodrigues",fullName:"Silvio Carlos Rodrigues",profilePictureURL:"https://mts.intechopen.com/storage/users/320125/images/system/320125.png",biography:"Silvio Carlos Rodrigues obtained a Ph.D. in Physical Geography from the University of São Paulo, Brazil, in 1998 and is a full professor at the Institute of Geography, Universidade Federal de Uberlândia (UFU), Brazil. He served as chair of the UGB in 2005–2006, secretary of the COMLAND of the IGA in 2010–2012, a full member of the CNPQ Advisory Committee on Physical Geography (CA-SA) in 2008–2010, and a full member of the Chamber of Natural Resources (CRA) with FAPEMIG in 2014–2016. Dr. Rodrigues is editor-in-chief of Sociedade & Natureza and a member of UNESCO\\'s EOLSS International Editorial Council. He is also a reviewer for the Brazilian Journal of Geomorphology, Caminhos de Geografia, and Environmental Earth Sciences. He has experience in geosciences, with emphasis on geomorphology, acting mainly on the following themes: geomorphology, geomorphological cartography, soil erosion, integrated environmental analysis, and geomorphological mapping.",institutionString:"Federal University of Uberlândia,",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Federal University of Uberlândia",institutionURL:null,country:{name:"Brazil"}}},coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"676",title:"Soil Degradation",slug:"soil-degradation"}],chapters:[{id:"74451",title:"RGB Spectral Indices for the Analysis of Soil Protection by Vegetation Cover against Erosive Processes",doi:"10.5772/intechopen.95055",slug:"rgb-spectral-indices-for-the-analysis-of-soil-protection-by-vegetation-cover-against-erosive-process",totalDownloads:401,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The vegetation cover plays a fundamental role in protecting the soil from erosive processes. Many researchers have developed investigations for the calculation of the RUSLE C Factor, with the use of operating bands in the near infrared. With the current advances in Geospatial Technologies, there are a good number of RGB airborne sensors in Unmanned Aerial Vehicles (UVA). The objective of this chapter is to evaluate some RGB indexes, proposed in the literature, for the protection of the soil from erosive processes by vegetation cover, in a region with a high agricultural vocation. The methodology consisted of capturing RGB images in an area of the Ecuadorian coastal region and calculating in thematic indices, within the visible one, which offer the possibility of quickly differentiating vegetation from other types of coverage on the ground. The evaluation allowed to define which indexes present the best results and adaptation to the type of crop or plant mass mapped, and to propose their use for zoning of risk of erosion under the agro-ecological conditions of the study area.",signatures:"Henry Antonio Pacheco Gil and Argenis de Jesús Montilla Pacheco",downloadPdfUrl:"/chapter/pdf-download/74451",previewPdfUrl:"/chapter/pdf-preview/74451",authors:[{id:"326877",title:"Ph.D.",name:"Henry Antonio",surname:"Pacheco Gil",slug:"henry-antonio-pacheco-gil",fullName:"Henry Antonio Pacheco Gil"},{id:"343735",title:"Dr.",name:"Argenis",surname:"de Jesús Montilla Pacheco",slug:"argenis-de-jesus-montilla-pacheco",fullName:"Argenis de Jesús Montilla Pacheco"}],corrections:null},{id:"75239",title:"Spatial Estimation of Soil Erosion Risk Using RUSLE/GIS Techniques and Practices Conservation Suggested for Reducing Soil Erosion in Wadi Mina Catchment (Northwest, Algeria)",doi:"10.5772/intechopen.96190",slug:"spatial-estimation-of-soil-erosion-risk-using-rusle-gis-techniques-and-practices-conservation-sugges",totalDownloads:363,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"To meet the pressing water needs in Algeria, the state has put in place a strategy consisting of the creation of hydraulic infrastructure for the mobilization of surface water resources. In fact, 74 dams are currently in operation; these structures are silting up at a rapid pace, generating an estimated annual loss of 45 million m3. Sidi Mhamed Benaouda dam located in the Oranian hill, with a water capacity of respectively 241 million m3 plays a crucial economic role in this region. The protection of this dam against erosive processes is a pressing economic goal. To do this, the RUSLE/GIS approach was used to map the erosive hazard. The results obtained in the Mina catchment, following a subdivision of 1315 homogeneous land parcels, show a total annual loss of 60 million tons/year with an average loss of 11.2 t/ha/year. About 50% of the catchment area was predicted to have very low to low erosion risk, with soil loss between 0 and 7.4 t/ha/year. Erosion risk is moderate over 13.9% of the catchment, where calculated soil loss is between 7.4 and 12 t/ha/year. Erosion risk is high to dangerous over 36.1% of the catchment, where calculated soil loss is more than 12 t/ha/year. According to this study, it appeared clearly that we must intervene quickly by using reliable and effective conservation techniques.",signatures:"Ahmed Benchettouh, Sihem Jebari and Lakhdar Kouri",downloadPdfUrl:"/chapter/pdf-download/75239",previewPdfUrl:"/chapter/pdf-preview/75239",authors:[{id:"308992",title:"Prof.",name:"Sihem",surname:"Jebari",slug:"sihem-jebari",fullName:"Sihem Jebari"},{id:"314333",title:"Dr.",name:"Ahmed",surname:"Benchettouh",slug:"ahmed-benchettouh",fullName:"Ahmed Benchettouh"},{id:"325116",title:"Prof.",name:"Lakhdar",surname:"Kouri",slug:"lakhdar-kouri",fullName:"Lakhdar Kouri"}],corrections:null},{id:"74771",title:"Remote Sensing and GIS-Based Soil Loss Estimation Using RUSLE in Bahir Dar Zuria District, Ethiopia",doi:"10.5772/intechopen.95393",slug:"remote-sensing-and-gis-based-soil-loss-estimation-using-rusle-in-bahir-dar-zuria-district-ethiopia",totalDownloads:535,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The severity of soil loss in the Ethiopian highlands has been increased from time to time. Hence, the assessment of soil erosion using models is very important for planning successful and sustainable soil management. This study was conducted in Bahir Dar Zuria district, Ethiopia with aiming to quantify the amount of soil loss using the GIS-based RUSLE (Revised Universal Soil Loss Equation) model. Based on the study, the most pronounced RUSLE factor that increases soil erosion was the slope length (L) and slope steepness (S). Compared with other land uses, bare land and cropland in the higher slopes were more vulnerable to erosion. As expected slope and soil losses have a direct relationship. About 80% of the study area experienced annual soil loss of less than 1.2 ton/ha/yr. Conversely, soil loss was very high for slopes greater than 30%. This indicated that slope has a great impact on regulating soil loss. The annual soil loss for cropland, vegetation, grassland, and degraded land was 19.05, 8.78, 8.82, and 71.16 ton/ha/yr., respectively. This is to means that land use land cover have a strong relationship with the amount of soil loss. The same land cover with different slopes have different soil loss amount. It was found that lack of vegetative cover during the critical period of rainfall, expansion of croplands, and absence of support practices increase soil erosion. Thus, the application of stone lines, contour tillage, terraces, and grass strip barriers are suggested to break the slope length into shorter distances, reducing overland flow velocity and soil erosion. Moreover, improving the awareness of society to reduce the illegal cutting of trees and apply conservation practices to reduce soil erosion in their farmland is very essential.",signatures:"Nurhussen Ahmed Mohammed and Desale Kidane Asmamaw",downloadPdfUrl:"/chapter/pdf-download/74771",previewPdfUrl:"/chapter/pdf-preview/74771",authors:[{id:"286238",title:"Dr.",name:"Desale Kidane",surname:"Asmamaw",slug:"desale-kidane-asmamaw",fullName:"Desale Kidane Asmamaw"},{id:"323673",title:"MSc.",name:"Nurhussen Ahmed",surname:"Mohammed",slug:"nurhussen-ahmed-mohammed",fullName:"Nurhussen Ahmed Mohammed"}],corrections:null},{id:"74627",title:"Determination of the Most Priority Conservation Areas Based on Population Pressure and Erosion Hazard Levels in Lesti Sub-Watershed, Malang Regency, Indonesia",doi:"10.5772/intechopen.95386",slug:"determination-of-the-most-priority-conservation-areas-based-on-population-pressure-and-erosion-hazar",totalDownloads:324,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"In a watershed, the Erosion Hazard Level (EHL) is usually associated with erosion rate and existing soil solum. In Lesti Sub-Watershed, erosion rate increases every year due to erosivity factor, erodibility, the length and slope, as well as crop factor and land conservation action. Analysis of erosion associated with population pressure has not been much discussed in the Lesti Sub-Watershed. This topic needs to be explored given that the erosion rate that affects sedimentation in the Sengguruh Reservoir, as an outlet of the Lesti Sub-Watershed, cannot be separated from the population activity therein. The population activity and the choice of use of land suppress the land so that it affects the carrying capacity of the watershed. Measuring land strength is usually based on the value of existing population pressure and its effect on vulnerability or erosion hazard level. This study seeks to assess the relationship between erosion hazard level and population pressure, as well as to determine the priority conservation areas in the Lesti Sub-watershed. The research approach uses a mixed method. The results shows that from 12 sub-districts in Lesti Sub-watershed there is 1 sub-district which has high population pressure as well as severe EHL. This sub-district is the most priority area for environmental conservation.",signatures:"Andi Setyo Pambudi",downloadPdfUrl:"/chapter/pdf-download/74627",previewPdfUrl:"/chapter/pdf-preview/74627",authors:[{id:"323619",title:"M.Sc.",name:"Andi Setyo",surname:"Pambudi",slug:"andi-setyo-pambudi",fullName:"Andi Setyo Pambudi"}],corrections:null},{id:"74327",title:"The Impacts of Soil Degradation Effects on Phytodiversity and Vegetation Structure on Atacora Mountain Chain in Benin (West Africa)",doi:"10.5772/intechopen.93899",slug:"the-impacts-of-soil-degradation-effects-on-phytodiversity-and-vegetation-structure-on-atacora-mounta",totalDownloads:363,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Atacora mountain is a particular ecosystem of West Africa where soil degradation occurs. The present study assessed the impacts of physical soil degradation on vegetation in the Beninese portion of this mountain chain. Phytosociological surveys were carried out along line transects from plain to summit within 22 plots of 30 m x 30 m. Based on indicators of physical soil degradation each plot was classified into one soil degradation class (Light, Moderate, High or Extreme). Impacts on plant diversity were assessed by comparing the floristic composition of soil degradation classes with the index of similarity of Jaccard. Variations between soil degradation classes of species richness, species chorological types, species life forms and species dispersal were also tested using a discriminant analysis combined with ANOVA. The Multi-Response Permutation Procedures analysis was used to pairwise compare the soil degradation classes based on the cover data of the species lists. All soil degradation classes were dissimilar, depending on the floristic composition. Discriminant analysis and ANOVA performed on biodiversity indicators had shown that species richness, and the number of regional species, phanerophytes and sarcochory decreased along the increasing degradation gradient in contrast to the number of species with wide distribution, therophytes and sclerochory. With regard to vegetation structure, the results had shown that only moderately and highly degraded soils presented the similar vegetation type. Physical soil degradation induced modification of floristic composition, phytodiversity loss and modification of vegetation structure. These results showed that the soil degradation gradient corresponds to a vegetation disturbance gradient.",signatures:"Farris Okou, Achille Assogbadjo and Brice Augustin Sinsin",downloadPdfUrl:"/chapter/pdf-download/74327",previewPdfUrl:"/chapter/pdf-preview/74327",authors:[{id:"209358",title:"Prof.",name:"Brice Augustin",surname:"Sinsin",slug:"brice-augustin-sinsin",fullName:"Brice Augustin Sinsin"},{id:"325174",title:"Ph.D.",name:"Farris",surname:"Okou",slug:"farris-okou",fullName:"Farris Okou"},{id:"326994",title:"Prof.",name:"Achille",surname:"Assogbadjo",slug:"achille-assogbadjo",fullName:"Achille Assogbadjo"}],corrections:null},{id:"75436",title:"Erosion Control Success Stories and Challenges in the Context of Sustainable Landscape Management, Rwanda Experience",doi:"10.5772/intechopen.96267",slug:"erosion-control-success-stories-and-challenges-in-the-context-of-sustainable-landscape-management-rw",totalDownloads:336,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"The Government of Rwanda sets up a conducive policy environment to invest in several development initiatives. Agriculture sector as the main contributor in the economic development received supports to sustainably manage Rwandan hilly landscape, dominantly ranging from 5 to 55% slope gradient. Intensive erosion control interventions confronted with different approaches have been introduced in the country such as participatory landscape management, (participatory) integrated watershed management and site-located intervention without any specified approach. This chapter intends to describe and evaluate the impacts of these previous approaches used in Rwanda in order to retrieve the success stories and encountered challenges as lessons learnt in the future interventions for optimizing land productivity in a sustainable manner. Participatory landscape approach in Gishwati area was a success story in protecting degraded lands and generating ecosystem benefits. It leads to more sustainable natural resources management from participatory planning up to implementation which addressed the frequent landslides, erosion and flooding while sustainably exploit the land to the profit of local farmers in the livelihoods. About 6,600 ha of lands have been successfully protected with full-packaged bench terraces, rangeland blocks and forest regeneration. This participatory approach also helped to relocate people from high risk zones to other safe places and build capacities of farmers through farm-livestock cooperatives. On the other side, Nyanza and Karongi sites under LWH project also emphasized strong evidences how land husbandry technologies (terraces) efficiently reduced erosion risks and improved farmers’ livelihoods. Lands were made productive with implementation of bench terraces on 3212 and 2673 hectares respectively for the two selected sites. However, challenges were observed from technical and socio-economic contexts which might have caused farmers to abandon or under-exploit the terraced lands. Finally, the chapter suggests to scale up the participatory landscape management approach which supports the involvement of farmers’ communities in the process.",signatures:"Jules Rutebuka",downloadPdfUrl:"/chapter/pdf-download/75436",previewPdfUrl:"/chapter/pdf-preview/75436",authors:[{id:"324918",title:"Dr.",name:"Jules",surname:"Rutebuka",slug:"jules-rutebuka",fullName:"Jules Rutebuka"}],corrections:null},{id:"76192",title:"Biochar: A Sustainable Approach for Improving Soil Health and Environment",doi:"10.5772/intechopen.97136",slug:"biochar-a-sustainable-approach-for-improving-soil-health-and-environment",totalDownloads:427,totalCrossrefCites:2,totalDimensionsCites:2,hasAltmetrics:0,abstract:"Current agriculture faces multiple challenges due to boom in food demand and environmental concerns. Biochar is increasingly being recognized by scientists and policy makers for its potential role in carbon sequestration, reducing greenhouse gas emissions, renewable energy, waste mitigation and as a soil amendment. The purpose of this review is to provide a balanced perspective on the agronomic and environmental impacts of biochar amendment to soil. Application of biochar to soil can play a significant role in the alteration of nutrients dynamics, soil contaminants as well as microbial functions. Therefore, strategic biochar application to soil may provide agronomic, environmental and economic benefits. Recent findings also supported that in order to enhance crop yield, improve soil quality and soil health, biochar has proven significant role as fertilizer and soil conditioner respectively.",signatures:"Shreya Das, Samanyita Mohanty, Gayatri Sahu, Mausami Rana and Kiran Pilli",downloadPdfUrl:"/chapter/pdf-download/76192",previewPdfUrl:"/chapter/pdf-preview/76192",authors:[{id:"329835",title:"Assistant Prof.",name:"Gayatri",surname:"Sahu",slug:"gayatri-sahu",fullName:"Gayatri Sahu"},{id:"340032",title:"Ms.",name:"Shreya",surname:"Das",slug:"shreya-das",fullName:"Shreya Das"},{id:"355301",title:"Dr.",name:"Samanyita",surname:"Mohanty",slug:"samanyita-mohanty",fullName:"Samanyita Mohanty"},{id:"355302",title:"Ms.",name:"Mausami",surname:"Rana",slug:"mausami-rana",fullName:"Mausami Rana"},{id:"355303",title:"Mr.",name:"Kiran",surname:"Pilli",slug:"kiran-pilli",fullName:"Kiran Pilli"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"3854",title:"Environmental Risk Assessment of Soil Contamination",subtitle:null,isOpenForSubmission:!1,hash:"88e43f7e0affb0c1ba3eccf8675e10f2",slug:"environmental-risk-assessment-of-soil-contamination",bookSignature:"Maria C. 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The evaluation of these patients necessitates effective imaging techniques in both diagnosis and long-term follow-up. Even though Cardiac Magnetic Resonance imaging is currently the imaging modality of choice for tissue characterization, advanced echocardiography represents a modern alternative. Speckle tracking echocardiography can be used to assess myocardial deformation at both segmental and global levels. Since distinct myocardial pathologies affect deformation differently, information about the underlying tissue can be offered by strain imaging. Echocardiography advances also show promising results in the improvement of diagnostic accuracy, management, and follow-up and a major advantage of echocardiography over other imaging modalities is the ability to use it in real-time, in the cardiac catheterization laboratory, allowing for the performance of imaging immediately before, during, and after interventional procedures. Furthermore, the prevalence of adult congenital heart disease continues to grow due to advances in surgical and diagnostic techniques. Echocardiography has proven to be a useful tool in the diagnosis and follow-up of these patients, both after percutaneous and surgical procedures, and its utility has expanded significantly due to the development of better technology. In addition, stress echocardiography could be useful in the evaluation of several cardiac diseases and should be preferred over other imaging modalities due to the lower cost, wider availability, and radiation-free nature.
\r\n\tThis book intends to provide the reader with a comprehensive overview of the current state-of-the-art novel imaging techniques by focusing on the most important evidence-based developments in this area.
Geography can be used to clarify the spatiotemporal distributions of various phenomena like climate, flora, fauna, human activities on the Earth, as well as their underlying mechanisms. In contrast, environmental engineering emphasizes the connections among individual elements such as air, water, soil, and living organisms, and attempts to link research findings to policy-making and technical development.
\nThe author wants to promote environmental engineering studies to achieve urban design that incorporates environmental factors and fosters the diversity of natural ecosystems, with a focus on Asian cities, which are experiencing the fastest urbanization rate in the world. The author will show how urban physical forms can be designed to take advantage of surrounding natural environments, while also considering specific political and social systems. Another aim is to contribute to global urban design by helping to shift the paradigm of urban planning through innovations that will make low-carbon cities a reality.
\nUrban areas receive energy, water, products, and raw materials from the outside and emit other products, wastes, sewage, exhaust gases, and waste heat. These waste products are often emitted directly into the environment. This process is called “urban metabolism” in reference to its similarity to the metabolism of a living organism [1, 2]. Mitigating the environmental burden of urban activities requires optimizing the structure of urban metabolism. For example, incineration and heat pumps make it possible to recover heat from wastes and sewage, and the effective use of such untapped energy sources will not only reduce the environmental burden by cycling the energy flow within urban areas, but will also more effectively use energy and resources.
\nIn this chapter, the author focuses on urban area and shows case studies on urban energy planning and urban climate induced by concentrated human activities like energy use based on geographic information like land use data and numerical climate model linked to GIS (Figure 1). These outputs will give some sustainable urban design in viewpoint of climate change (mitigation and adaptation).
\nConceptual structure of this chapter.
Waste incineration plants supply far more concentrated heat than can sewage plants that produce energy, but incineration plants tend to be isolated so they can naturally serve only limited areas. With sewage, heat pumps make it possible to recover heat by taking advantage of the temperature difference between air and sewage. Tokyo (meaning the 23 special wards of the city proper) is covered by a sewerage network [3] (Figure 2); although the sewage in this network provides heat distributed widely in a low quality, heat recovery and supply are expected to be possible in broad areas. For such heat utilization to work, there must be quantitative congruence between heat demand and the use of waste heat in heat supply operations. It would seem to be useful to search for such areas, and using a GIS may facilitate this process.
\nExtracted sewerage lines and sewerage treatment districts in the 23 special wards of Tokyo Metropolis [
Connolly et al. [5] and Manfren et al. [6] reviewed computer tools that can be used to analyze the integration of renewable energy, including the currently untapped (and diffuse) sources of city energy. Some of the tools are GIS-based and applicable at the local community scale [7, 8, 9, 10], but their target is mainly electricity (solar, wind, and biomass burning). Gils et al. [11] introduced a methodology for a GIS-based analysis of district heating potential. They assessed the energy demand for space heating and hot water in residential and commercial sectors in U.S. cities. The author’s interest is the spatial conformity between energy demand and supply when referring to community-scale urban structure. This point is the reason why the author think GIS analysis is applicable for urban energy planning.
\nAccordingly, in relation to established plans of district heat supply facilities that make effective reuse of heat obtained from sewage, the author developed the GIS software to perform analyses of the spatial congruence between heat demand and the use of waste heat in heat supply operations [4]. The temporal and spatial distribution structure of energy consumption [12] was used as the basic data for the GIS, as was the distribution information for land-use types, height of buildings, and other relevant items used in the approximations.
\nThe sewer system simulation model (SSSM) uses raster data on land-use types and building height (number of floors), and polygon data of sewage collection areas defined by sewerage lines, as inputs for performing calculations and mapping [4]. Main data entered into the model included floor space by type of building use for each 250 × 250 m grid cell [13], energy consumption per unit floor space according to industry type [12], and heat demand data according to type of building used [12, 14]. This model can calculate heat demand in each grid cell (Figure 3), calculate the sewage flow rate at sewerage line nodes (Figure 2), and calculate the amount of recoverable sewage heat at the nodes and surrounding grid cells (Figure 4).
\nDistribution of heat demand density in Tokyo (daytime, winter, grid size: 250 m) [
A schematic diagram of theoretical calculation results for recoverable heat at the points along a sewerage line [
In the areas with a large amount of usable heat, there are long sewerage lines and many possible locations of heat recovery in general. Owing to the many possible locations of recovery, each with large heat demand in areas such as business districts, the amount of usable heat per heat pump is large. There were also a few cases where the flow rate was a limiting condition. When sewerage lines run through residential areas in main, the heat demand along the sewerage lines is small [4]. In finding the candidate places to settle district heat supply facilities that make effective reuse of heat obtained from sewage, the usage of GIS gives the reasonable solution in scientific view point.
\nExpansion of urban areas and increase in urban activity have several impacts on urban environments. These impacts are represented by air pollution, water pollution, thermal pollution, caused by anthropogenic heat emission, and so on. Anthropogenic heat affects local climate in urban areas producing phenomena such as heat islands [12]. This process has been explored in a numerical simulation [12, 15]. It was reported that urbanization has both positive and negative impacts on thermal environment, as an increase in urban energy use and a decrease in incoming solar radiation [16]. On the other hand, evaluation of the impact of urban activity through land use change and anthropogenic heat on the urban thermal environment has been necessary in view of urban planning. Quantitative analysis of how human activities and urban structures should be changed to reduce heat island phenomena requires detailed data on anthropogenic heat sources, one of the surface boundary conditions, for use in numerical simulations of such impacts. Such analysis needs evaluation of the influence of spatial and temporal structures of anthropogenic heat on urban thermal environment. Field surveys of energy consumption over wide areas are difficult to carry out. The data for energy consumption as a source of anthropogenic heat must be in the form of grid cell data to be referenced geographically. Some studies which mapped the anthropogenic heat in Tokyo have been published [12, 15]. A time-series analysis of the distribution of urban energy consumption is necessary to evaluate its impact on the thermal environment. The author attempted a detailed analysis of the temporal variability of the distribution of energy consumption in the 23 special wards of the Tokyo Metropolis (Tokyo) [12]. He used a digital geographic land use data set containing the number of floors of building at each grid point to tabulate energy consumption (Figure 5) on an areal basis. The numerical simulation of urban climate was consequently performed to determine the effect of anthropogenic heat on urban climate in Tokyo. Annual and diurnal variability of energy consumption (Figure 6) is necessary for computer mapping of urban anthropogenic heat.
\nAreal energy consumption for each category on business or land use in Tokyo in 1989 [
Diurnal variability of demand for space cooling in summer, space heating in winter and hot water supply in winter. Additionally diurnal variability of areal energy consumption in the manufacturing and transportation sectors is also shown [
The author drew detailed maps of anthropogenic heat in Tokyo with data from energy statistics and a detailed digital geographic land use data set including the number of floors of building at each grid point (Figure 7) [12]. Computer graphics animation of the diurnal and annual variability in anthropogenic heat of Tokyo was also prepared with the same data sources. These outputs characterize scenarios of anthropogenic heat emission and can be applied to a numerical simulation model of the urban climate [12]. The anthropogenic heat flux in central Tokyo exceeded 400 W m−2 in daytime, and the maximum value was 1590 W m−2 in winter. The hot water supply in offices and hotels contributed 51% of this 1590 W m−2. The anthropogenic heat flux from the household sector in the suburbs reached about 30 W m−2 at night. Numerical simulations of urban climate in Tokyo were performed by referring to these maps. A heat island appeared evident in winter because of weakness of the sea breeze from Tokyo Bay. At 8 p.m., several peaks of high-temperature appeared, around the areas with the largest anthropogenic heat fluxes (Figure 8). In winter, the shortwave radiation was weak, and the influence of anthropogenic heat was relatively large.
\nDistribution of anthropogenic heat in Tokyo at 2 p.m. (upper) and 9 p.m. (lower) in winter [
Horizontal distribution of air temperatures (°C) and wind system at 7.5 m above the ground in winter midnight [
The spatiotemporal data in high resolution on human activity like energy consumption in urban area enable us to evaluate the impact of anthropogenic heat to urban climate by a numerical simulation model of the local climate.
\nThe following progress of RS and GIS technology brought a more advanced application of the author’s approach. Hirano et al. [17] simulated local climate with surface boundary conditions based on satellite RS data. Most previous mesoscale meteorological modelling studies use land-use data instead as the surface boundary conditions. However, small patches of vegetation cover, such as garden trees and roadside trees, are excluded from the land-use data. Therefore, they made a fractional vegetation cover (FVC) data set with these small patches of vegetation cover from RS data (Figure 9), and then simulated the urban heat island in Tokyo with FVC data as new surface boundary conditions. The air temperature with the new boundary condition is up to 1.5°C lower than that with the old one (Figure 10). Furthermore, the new boundary condition led to predicted air temperatures closer to the measured temperatures than those with the old one. Therefore, it is important for urban climate simulations to include small vegetation cover.
\nEstimated FVC (Fractional Vegetation Cover) around Tokyo [
Temperature difference in °C between the case applying FVC data and the case not applying at 15:00 LST at 4.5 m above ground level [
The recent precise geographic information of urban vegetation cover derived from RS data enables us to evaluate the contribution of urban green to cool our community with higher accuracy.
\nAbout 120 years have passed since meteorological observations began in Asia; since then, researchers have used various methods to reconstruct the climate of earlier times. One such method has been to use notations about weather in ancient journals and documents. Since the early modern times, the Japanese have left many usable records, and, although these are mostly qualitative accounts, researchers have learned how hot in summer, or how cold in winter, it was in the past. Although the information collected from these documents contains many spatial and temporal gaps, recent advances in computerized meteorological modelling have made it possible to compute local distributions of climatologic parameters; for example, the distribution of surface air temperature is determinable if the surface boundary conditions, like the land-use distributions, are known [12, 15]. The author used numerical simulations with a mesoscale climate model referenced to digital land-use data (2 km grid) covering all of Japan to attempt to isolate the influence on surface air temperature of regional warming related to land-use change during a recent 135-year period [18]. During this period, areas of regional warming related to land use changes expanded around Osaka and Tokyo. However, the validation of the results of this study was difficult because the amount of available data on long-term climate fluctuations was limited.
\nTo validate the results of modelling such as the author’s [18], observed climatologic data (e.g., air temperature) are needed to provide initial values for the modelling. The weather stations’ network and other observation facilities that have been established throughout Japan since 1876 provide long-term temperature fluctuations to the present. Data for Tokyo have often been used to associate changes in local climate in conjunction with urbanization [19]. Many instances of global warming [20] and changes of air temperature due to the regional climate change have been identified over broader areas and longer periods than those caused by local land-use changes [21]; comparing these to modeled temporal changes in air temperatures may elucidate the contribution of land-use change to climate change in Japan. Comparisons like this will also help to overcome the problem of spatial and temporal discontinuity in historical climatologic information.
\nAnother reason such research is important in Japan is that urbanization in large Japanese cities has increased local air temperatures by about 1°C during the twentieth century [22]. Until now researchers have relied on statistical methods to distinguish between the influence on air temperature of localized warming caused by urbanization, and the influence of global warming and other broader scale changes of air temperature [23]. Of course, such methods cannot be applied to time periods or regions without any observed data. Much pioneering research has eliminated the influence of urbanization as noise when estimating temperature changes over large areas [24]. The use of a mesoscale model with past land-use distributions as surface boundary conditions to simulate past climatologic parameters (e.g., air temperature distributions) might help to distinguish the influence of localized warming due to urbanization from that of global warming and regional-scale (e.g., Far East Region, Eastern Asia, etc.) fluctuations of air temperature for time periods and regions without enough observed data [18]. Certainly, the urban-scale and mesoscale meteorological model have been widely used in other studies, such as Kusaka et al. [25] and Doan et al. [26], which created the weather research and forecasting model (WRF) to examine the phenomena of urban climate in different scales.
\nCase studies of the area around the upper reaches of the Rhine River as it was in 1710 [27] and of the Edo area (Tokyo was called “Edo” until the middle of the nineteenth century) during the first half of the nineteenth century [18] are examples of attempts to quantify the influence of urbanization-induced changes in the surface heat budget on surface air temperatures, as opposed to research from a historical climatology perspective. These researchers derived land-use distributions for the periods they studied from historical maps and other historical sources. Because the periods they studied predate the beginning of meteorological records, comparisons with observed meteorological data are impossible.
\nThe author is focusing on both the past ground surface temperatures (Tsfc) of 100 years ago and the current one, in order to clarify the relationship between the urban development and subsurface warming in seven Asian megacities based on the numerical simulation of local climate, considering the land-use data in these megacities for three discrete years of the twentieth century [28], because subsurface temperatures are affected by surface warming (heat of the ground level is conducted downwardly). The author used the Colorado State University Mesoscale Model (CSU-MM) [29, 12, 18] and digital land-use data (2 km grid) from seven Asian megacities (Seoul, Tokyo, Osaka, Taipei, Bangkok, Manila, and Jakarta) to simulate Tsfc for three discrete years of the twentieth century (Figure 11).
\nUrbanization around seven megacities in Asia (RIHN Research Project C-5 “Human Impacts on Urban Subsurface Environments”) [
Based on the vertical profile of subsurface temperature in boreholes, which is regarded to record the past Tsfc, Taniguchi et al. [30] identified surface warming during the twentieth century of 2.8°C in Tokyo and 1.8°C in Bangkok. The seven land-use data sets (Figure 11) revealed the major characteristics of land-use change during the twentieth century expansion of urbanized areas in the seven megacities considered here. Such large-scale surface changes would be expected to bring about localized climatic changes in response to the resultant changes to the surface heat budget. In the modelling on a calm and clear day of the hottest season, the central Bangkok showed Tsfc to be increasing by about 1.1 K 100 y−1 during the twentieth century [28] (Figures 12 and 13).
\nLand-cover/use around Bangkok in 1910 with its legend [
Computed Tsfc (K) around Bangkok in the hottest season (calm and clear day at the end of March) [
There is almost no difference between the graphs of diurnal change of Tsfc between 1960 and 2000 in the city center of Bangkok (Figure 14), whereas in the northern suburbs of Bangkok, where land use between 1960 and 2000 has changed from rice paddy to urban, there has been a rapid increase of Tsfc. In particular, Tsfc in the northern suburbs of Bangkok in 2000 was higher than in 1960, by about 15 K in the daytime and by about 5 K at dawn. Thus, it appears that subsurface temperature profiles differ according to the start time of urbanization periods.
\nDiurnal variability of computed Tsfc in the hottest season (calm and clear day) in two stages [
Thus, the author’s approach is available not only to evaluate the current urban environment but also to the past one. If a certain scenario of future’s urban land use and urban activity, this approach will be available for the future prediction.
\nIn this chapter, the author focuses on urban area and shows case studies on urban energy planning and urban climate induced by concentrated human activities like energy use based on geographic information like land use data and numerical climate model linked to GIS. The author shows the following four outcomes: (1) In finding the candidate places to settle district heat supply facilities that make effective reuse of heat obtained from sewage, the usage of GIS gives the reasonable solution in scientific view point, (2) The spatiotemporal data in high resolution on human activity like energy consumption in urban area enable us to evaluate the impact of anthropogenic heat to urban climate by a numerical simulation model of the local climate, (3) The recent precise geographic information of urban vegetation cover derived from RS data enables us to evaluate the contribution of urban green to cool our community with higher accuracy, (4) The author’s approach is available not only to evaluate the current urban environment but also to the past one. If a certain scenario of future’s urban land use and urban activity, this approach will be available for the future prediction. Thus, these outputs will give some sustainable urban design in viewpoint of climate change (mitigation and adaptation).
\nThe author sincerely thanks to Mr. Kai Liu, Research Assistant of National Institute for Environmental Studies for his great contribution to the editorial process of this manuscript. The author states that some contexts and figures of this manuscript come from his previous publications like [4, 12, 17, 28] and are permitted to use.
\nThe author declares there is no conflict of interest on this manuscript.
Cervical cancer is the fourth most prevalent cancer among women worldwide. Human Papillomavirus (HPV) is known to be the main cause of cervical cancer [1]. It is well-known that cervical cancer has long latency period. Pre-malignant abnormalities in cervical cells can take up to a decade to progress to carcinoma. Early diagnosis of pre-cancerous cervical cells and treatment help in halting the progression of this fatal cancer [2]. The five year survival rate for patients suffering from cervical cancer has been documented to be over 60% [3]. In spite of the long latency period for cervical cancer to develop, the mortality rate among cervical cancer patients is high in developing countries due to shortage of skilled clinicians and lack of effective screening tools [4, 5].
The cervix which is the innermost part of uterus is sub-divided between the endo-cervix and ecto-cervix regions. The endo-cervix is composed of glandular cells whereas the ecto-cervix is made up of squamous cells. Transformation zone is the place where these regions adjoin, and where most of the cervical cancer is known to originate [6]. Starting with the transformation zone the cells in the squamous region are typically classified as basal, para-basal, intermediate and superficial respectively. The majority of cells in a typical Pap-smear cell sample used for examination by clinicians are from the intermediate and superficial outer layers. The classification of precancerous cells as low grade and high grade is established through Bethesda system [7, 8] which is based on morphological changes in the cells (particularly the cell nuclei). Traditionally the detection of precancerous cervical cells is primarily performed using cytological screening. The widespread usage of liquid based cytology (LBC) in recent years has made the process of sample preparation and examination more uniform. However, screening methods based on visual inspection can suffer from both inter- and intra-observer variability. Machine learning based approaches have gained attention in this regard [9, 10, 11, 12, 13] and standard benchmark datasets of cervical cell images have been created [14, 15]. The goal of machine learning approaches is to make the process of cell classification at least semi-automated and to provide an assisting tool for cyto-pathologists.
The machine learning based studies have focused mainly on the 2D bright-field images of the cervical cells and their nuclei. Since cells are 3D objects, we believe that additional morphological information in the third (depth) dimension of cells, if available, can provide new information and help any image based cell classification. Digital Holographic Microscopy (DHM) is an interferometric imaging technology [16, 17, 18] which can fill this gap and provide quantitative phase information that may then be related to the depth dimension of the cells. When a coherent beam of light of wavelength
Here n(x,y,z) represents the refractive index distribution within the cell relative to its surroundings. It is important to understand that phase provides new non-redundant information that cannot be derived from the usual 2D bright-field images. The phase change
Traditional DHM systems are based on single-shot off-axis interference configuration or the multi-shot phase shifting con-figuration. The single-shot off-axis systems are simpler and cheaper to build but the conventional Fourier filtering approach for phase reconstruction in these systems leads to sub-optimal phase resolution. The multi-shot phase shifting configurations offer full resolution but are hardware intensive and require stringent vibration isolation thus making them difficult to employ in clinical settings. In recent years our group has developed optimization based phase reconstruction algorithms [24, 25, 26, 27] for single-shot DHM systems which offer the simplicity of hardware without compromising on resolution and quantitative phase accuracy. The full diffraction-limited resolution capability of our system allows us to treat the bright-field and phase images on par (with respect to their lateral resolution). The single-shot operation also reduces the cost of building a DHM system making it more accessible for wider deployment. Based on our imaging study we find that the phase images contain important morphological information associated with different classes of normal as well as abnormal cells. Further this phase information is seen to be robust across the samples from three clinical sites. Also the samples consisted of age group of 17–60 years of the subjects. The cell morphology captured as numerical parameters from the phase images can provide valuable additional information to clinicians over what they usually access with routine bright-field microscopy. Our results suggest that phase imaging can become an important clinical modality, and it should be possible to design phase-based software tools for clinicians to make better informed decisions with this new information. The Chapter is organized as follows. In Section 2 we explain the technique of digital holographic microscopy (DHM) and the nature of quantitative phase images along with our phase reconstruction methodology. Section 3 briefly describes the details of the cell samples used. The results are discussed in Section 4. In Section 4.1 we start by showing images of cervical cells in both bright-field and phase modalities to illustrate morphological changes in cervical cell nuclei. This is followed by PCA analysis of the cell data based on the morphological parameters derived from the cell images in Section 4.2. In Section 4.3 we describe our analysis to understand if the most important phase parameters for normal cells are consistent across different patients from same clinical site, across different clinical sites and between different age groups. Finally in Section 5 we provide concluding remarks.
DHM is an interferometric modality where the recorded image data
Here
Reconstruction of single-shot holograms is traditionally performed using the Fourier transform method. However, due to the low-pass filtering nature of this method image plane phase recoveries with full pixel resolution cannot be obtained using this approach. This poses a problem as the bright-field images available will then seem to have higher resolution even though both have been recorded using the same microscope objective. In order to have both bright-field and phase images with same diffraction-limited resolution, we reconstruct of the complex object wave
Here
The tuning parameter
and
It is important to note that the optimization procedure operates fully in the image domain making it possible to employ it over a region of interest and thus allowing full resolution reconstruction in near real time. In our study, a
Processing method | Single/Multi-shot | Resolution |
---|---|---|
Fourier filtering | Single-shot | Low resolution |
Phase shifting | Multi-shot | Full diffraction-limited |
Optimization | Single-shot | Full diffraction-limited |
Summary of resolution performance of image plane digital holographic methods.
The phase map
Since the arctangent function is defined only over the range
Steps in imaging chain (a) Pap-smear slide, (b) dual-mode DHM system, (c) illustrative example of a bright-field image and a hologram recorded using the DHM system, (d) computer used for reading image from camera, phase reconstruction and computing morphological parameters from the bright-field and phase images, (e) illustrative example of phase map of a cell nucleus rendered as a surface plot.
Modality | Parameter | Parameter descriptiona |
---|---|---|
Area | ||
Nucleus perimeter | Number of boundary pixels of M | |
Mean intensity of | R, G, B componentsAverage R, G, B values computed over mask | |
Variance of | R, G, B componentsVariance of R, G, B values computed over mask | |
N/C Ratio | Ratio of areas (Nucleus)/(Cytoplasm) Labels assigned: low =1, mid =2, high = 3 | |
Mean and maximum phase | ||
Optical volume | (Area) | |
Variance of phase | Variance | |
Roughness at scales | 1, 0.75, 0.5, 0.25 | |
Moment of inertia | of nucleus | |
Shift between geometric and phase centroid |
Morphological parameters evaluated for each cell nucleus imaged in this study. More details about these parameters are provided in Table 1 of ref. [23].
M denotes a binary (0, 1) mask for individual cell nucleus, q denotes phase map. Both are defined over ROI of 256 × 256 pixels centered on cell nucleus.
We imaged a total of 48,006 cervical cells from 291 Pap-smear slides from three different hospitals in Delhi: AIIMS (All India Institute of Medical Sciences, New Delhi), LHMC (Lady Hardinge Medical College, New Delhi) and MAMC (Maulana Azad Medical College, New Delhi) (see Table 3). The samples were upto three years old (not from current patients) and stored in the repositories at the respective sites. They were collected by following the standard protocols for the Pap-smear examination. The patients varied in age from 16–70 years and came from varied geographical locations in India. The cell samples can be prepared conventionally or with Liquid-based cytology (LBC). For each method staining is performed with Pap stain for visualization with bright-field microscopy. For our study we have used both types of samples. Two of the sites used liquid based cytology (LBC) slides prepared via ThinPrep and SurePath systems; while third site used conventional Pap-smears. In LBC method, samples are collected in liquid vials and the slide is prepared semi-automatically. The advantage of LBC is uniformity in sample preparation. On the other hand in conventional cytology the sample is applied directly to a slide for microscopic investigation. Table 3 provides details about number of cells imaged. The classification of normal vs. abnormal cells in these samples was provided based on the bright-field images by practicing cyto-pathologists (S. R. M., M. S.,K. A., S.S.). The normal cells here include the superficial and intermediate cells while the abnormal cells include LSIL, HSIL, SCC, ASC-H and ASC-US type of cells [31].
Clinical site | Preparation method | Number of patients normal / with condition a | Normal cells | Abnormal cells |
---|---|---|---|---|
AIIMS, New Delhi | Liquid based cytology | 66 / 33 | 18801 | 532 |
(ThinPrep) | ||||
LHMC, New Delhi | Liquid based cytology | 174 / 9 | 28007 | 214 |
(SurePath) | ||||
MAMC, New Delhi | Conventional | 6/ 3 | 438 | 14 |
Details about cell samples used for imaging from the three clinical sites.
As determined by clinicians.
In this section we begin by providing sample images of cervical cell nuclei that were obtained using our dual-mode DHM system. While quantitative information obtained in terms of morphological parameters is certainly important, a large number of clinical sites worldwide typically use visual examination of cells using a bright-field microscope for cell classification. The importance of changes in nucleus structure in cancer diagnosis is already well-known [32]. With the illustrative examples in this section, we wish to qualitatively describe the morphological features observed in quantitative phase images of cervical cell nuclei in various stages. The simultaneous presentation of bright-field images (that pathologists can correlate to) and the quantitative phase images as shown here is important in our opinion from the perspective of clinical users. It is important to note that our single-shot full resolution phase reconstruction technique allows us to observe the quantitative phase images with the same resolution as the bright-field images. The examples shown here also aim to illustrate that the information contained in the quantitative phase images is different in nature from that in the bright-field images. Quantitative analysis of the images using morphological parameters as described in Table 2 will be provided in the following sections. In Figure 2 we show illustrative examples of normal cells in the intraepithelial squamous layer. A progression from intermediate to superficial stages is shown in Figure 2(a)–(j) respectively. As a cell progresses from intermediate to superficial stage the chromatin in the cell nucleus is known to condense. The superficial cells are in the outermost layer of ecto-cervix and have highly condensed pyknotic nucleus. While in the bright-field images the area of the nucleus is progressively decreasing and the color of the nucleus gets darker from intermediate to superficial stages, the accompanying phase map of the nucleus is seen to get taller by approximately a factor of 2. This change in the optical height profile cannot be inferred from the 2D bright-field images and the phase map is therefore seen to provide new morphological information. Further, from the phase profiles we also see that the evolution of the cells from intermediate to superficial stage happens via a continuous change. Next in Figures 3–5 we examine the abnormal cell classes low grade squamous intraepithelial lesion (LSIL), high grade squamous intraepithelial lesion (HSIL) and squamous cell carcinoma (SCC) which progressively indicate higher grade abnormalities. The class LSIL consists of abnormal superficial and intermediate cells. Variable degrees of hyper-chromasia, nuclear size variation with coarsely granulated chromatin are identifiers of a typical LSIL cell. In the HSIL class, the degree of nuclear enlargement and hyper-chromasia is more than LSIL and the cells here are found in sheet-like aggregates. In both LSIL and HSIL cases the phase profiles of the cell nuclei are seen to have increased roughness or corrugations compared to the normal cells. In the SCC class which is considered to be a confirmed case of malignant cervical smear, the phase profile of the nucleus shows sharp narrow peaks with large phase values. It is once again important to note that the phase profile clearly provides new morphological information that is not readily available in the 2D bright-field images. Apart from the main classes above, the Bethesda system defines a class Atypical Squamous Cells ASC which include the samples that cannot be categorized as normal or abnormal typically. The nuclei in this class are typically larger in area, however, as seen in Figure 6, the average phase value in the nucleus is slightly lower compared to the LSIL, HSIL or SCC classes. In both ASC-US and ASC-H classes (Figure 6), the phase profile has undulations but the phase structure of ASC-H is flatter with lower average phase value in the nucleus as compared to that of the ASC-US cells. Further in some ASC nuclei, we observe local peaks in phase profile located near the nuclear boundary leading to a dip in the center. Finally we show rare abnormal cell cases in Figure 7 that include inflamed, reactive, moon-type, virus-infected and koliocytotic classes. Just a few examples of these rare cells were present in our sample set. The phase profile for all these types appears corrugated with lower average phase values except for the virus infected cell type. While only a few representative images of each cell type have been shown simultaneously in bright-field and phase mode, we clearly observe that the phase profile offers distinctive morphological features that are not currently utilized in the clinical practice. This new information if incorporated in cyto-pathological examination, can be potentially valuable to clinicians.
Maturation of cells in intra-epithelial squamous layer from intermediate to superficial is shown from (a)-(j) in both bright-field and phase modes. The phase maps correspond to the ROIs marked in the bright-field images. As the nuclei progress to superficial stage, the nucleus area gets smaller and the phase profile is seen to get taller by approximately a factor of 2.
Illustration of LSIL cells, the three columns show the bright-field image, selected ROI and the phase image of the ROI.
Illustration of HSIL cells, the three columns show the bright-field image, selected ROI and the phase image of the ROI.
Illustration of SCC cells, the three columns show the bright-field image, selected ROI and the phase image of the ROI.
Illustration of (a), (b): ASC-US cells and (c), (d): ASC-H cells, the three columns show the bright-field image, selected nucleus ROI and the phase image of the ROI.
Illustration of rare abnormal cell types: (a) inflamed, (b) reactive, (c) moon-type, (d) virus-infected and (e) koliocytotic; the three columns show the bright-field image, selected nucleus ROI and the phase image of the ROI.
A MATLAB based software was designed to compute a number of morphological parameters associated with cell nuclei that are listed in Table 2 from each of the cell nuclei imaged in bright-field as well as quantitative phase mode. The cell nucleus measurement data was therefore consisted of an (N × p) matrix with N = 48,006 and p = 20. Note that one parameter in the measurement set is the N/C ratio which was given three labels (low =1, mid =2, high =3) based on visual inspection of nuclei. This was done so that a number of cells of interest that appeared in clusters for which determining the cytoplasm boundary was difficult could be used in the analysis. All the other parameters were measured over the nucleus region in an automated fashion. For the present analysis, the cells were nominally labeled as superficial, intermediate and abnormal (including LSIL, HSIL, ASC-US, ASC-H, SCC) by practicing cyto-pathologists (S. R. M., M. S., K. A., S.S.). Since the number of abnormal cells was much smaller (1.6%) compared to the normal (superficial and intermediate) cells, a truncated data-set with 450 randomly selected cells from each of the three types (superficial, intermediate and abnormal) as per prior labelling was used to train the PCA. Denoting the truncated data matrix with 1350 rows and 20 columns (representing the measurements) with each column in standard form (zero mean and standard deviation 1) by
Here the superscript “T” stands for the transpose of the matrix
Data corresponding to 48006 cells projected onto the first two PCA vectors. The color coding of black, blue, and red corresponds to cells that were labeled by cyto-pathologists as superficial, intermediate and abnormal (all classes) respectively.
Examples of intermediate and abnormal cells falling well within the abnormal and intermediate regions of the PCA plot in
We further performed a leave-one-out analysis of the PCA for the cell data to determine which of the 20 measurements influenced the PCA scores the most [33]. If the PCA eigenvectors are arranged as columns of a matrix
The plot in Figure 8 thus corresponds to the first two columns of the score matrix
Morphological parameter | Modality | Relative Procrustes Distance |
---|---|---|
Moment of inertia | Phase | 1.0 |
Optical volume | Phase | 0.85 |
N/C Ratio | Bright-field | 0.83 |
Perimeter of nucleus | Bright-field | 0.79 |
Mean phase of nucleus | Phase | 0.76 |
Relative importance of numerical parameters using leave-one-out analysis applied to PCA.
Quantitative phase is not a standard clinical methodology for cell classification, however, as we showed in Section 4.2, quantitative phase may become an important modality to consider for future clinical use. It is therefore important to understand if the phase parameters for cervical nuclei are consistent across different subjects from same clinical site, age group of subjects or clinical sites with different sample preparation methodologies. Since our leave-one-out PCA analysis suggested that optical volume and moment of inertia are the most important phase parameters as explained in the previous section, we have plotted a few hundred randomly selected normal cells (superficial and intermediate) with respect to these phase parameters in Figure 10. In Figure 10(a) we show the plot for 200 normal cells each for five different patients from a single clinical site. Figure 10(b) shows the same plot for 200 cells each from three different clinical sites with different sample preparation protocols. In Figure 10(c) we show the plot once again for 500 normal cells for two different age groups (below and above 30 years). From these plots we observe that the normal cells from different categories as above show highly overlapping distributions for the most important phase parameters. We believe that this observation is very important for standardization and usage of quantitative phase imaging methodology in future clinical practice.
Verification of consistency of the two most important phase parameters (moment of inertia and optical volume) decided based on the leave-one-out analysis; (a) plot of 200 cells each for 5 patients from the same clinical site (AIIMS), (b) plot of 200 cells each from three clinical sites with different sample preparation protocols, (c) plot of 500 cells each for patients below and above 30 years of age. The numerical values of moment of inertia and optical volume are normalized to standard form (zero mean and standard deviation one).
In this section we briefly describe an interesting possibility of quantitative phase imaging of unstained cervical cell samples with two typical images of normal cells as shown in Figure 11. For unstained cervical cell samples, the Pap smear was prepared using the conventional method and cells were fixed with ethyl alcohol. While the staining protocols used in Pap-smear sample is a gold standard for diagnosis by cyto-pathologists, we note here that compared to stained cells, the phase signal observed from nuclei of unstained unprocessed cell samples is almost three times higher in magnitude. While interpretation of images of the unstained cells and their phase may require one to go through a learning process, the possibility of using unstained cell samples for diagnostic practice may offer an attractive alternative as the cell sample preparations will not involve any wet-lab processing and recurring costs associated with reagents.
Illustration of bright-field and phase imaging of unstained cervical cells: (a), (d): Bright-field images of unstained cells, (b), (e): Nucleus ROI selected from the bright-field image, (c), (f): Phase map of the unstained nuclei.
In conclusion we have reported an image based study of cervical cells at various stages using bright-field as well as quantitative phase microscopy. Over 48000 cells have been imaged individually. The phase images of the cell nuclei were reconstructed using an optimization approach that provided same resolution as the bright-field images. This image data-set may be valuable for future application development using advance machine learning methods. The visual inspection of images shows interesting features in the phase images as the cells evolve from intermediate to superficial stages with distinct features associated with abnormal cells. This finding based on visual inspection is confirmed in the PCA analysis of the morphological parameters of cells derived from both the bright-field and phase images of cell nuclei. A leave-one-out analysis applied to the PCA scores suggests that apart from the N/C ratio that has been used for identifying abnormal cells for decades, the other two parameters that influence the PCA the most are optical volume and moment of inertia of nucleus - both of which are derived from phase images. A consistency study suggests that the phase parameters associated with normal cells show highly overlapping distributions for multiple patients from same clinical site, for three clinical sites with different sample preparation protocols and for patients in two age groups. The consistency of phase parameter distributions for these cases further suggest that phase is a robust modality that can certainly be used in a standardized manner in clinical practice. We believe that quantitative phase may become an important imaging modality in addition to the bright-field imaging that is solely used in the current clinical practice. While this study has been performed for cervical cells we believe that our conclusions regarding importance of quantitative phase may possibly have a wider applicability.
The authors acknowledge financial support from Department of Science and Technology India (Award: IDP/MED/34/2016) and Biotechnology Industry Research Assistance Council India (Awards: FT/12/02(110)/15/0237 and BT/SIBRI45/733/17). The unstained sample shown in Figure 11 of this chapter was shared by Dr. R. Sankaranarayanan (RTI International India). Incubation support from FITT-IIT Delhi is gratefully acknowledged.
The authors declare no conflict of interest.
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Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. 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He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. 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He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. 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Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. 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He also obtained an MSc in Molecular and Genetic Medicine, and a Ph.D. in Clinical Immunology and Human Genetics from the University of Sheffield, UK. He also completed a short-term fellowship in Pediatric Clinical Immunology and Bone Marrow Transplantation at Newcastle General Hospital, England. Dr. Rezaei is a Full Professor of Immunology and Vice Dean of International Affairs and Research, at the School of Medicine, Tehran University of Medical Sciences, and the co-founder and head of the Research Center for Immunodeficiencies. He is also the founding president of the Universal Scientific Education and Research Network (USERN). Dr. Rezaei has directed more than 100 research projects and has designed and participated in several international collaborative projects. He is an editor, editorial assistant, or editorial board member of more than forty international journals. He has edited more than 50 international books, presented more than 500 lectures/posters in congresses/meetings, and published more than 1,100 scientific papers in international journals.",institutionString:"Tehran University of Medical Sciences",institution:{name:"Tehran University of Medical Sciences",country:{name:"Iran"}}},{id:"180733",title:"Dr.",name:"Jean",middleName:null,surname:"Engohang-Ndong",slug:"jean-engohang-ndong",fullName:"Jean Engohang-Ndong",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180733/images/system/180733.png",biography:"Dr. Jean Engohang-Ndong was born and raised in Gabon. After obtaining his Associate Degree of Science at the University of Science and Technology of Masuku, Gabon, he continued his education in France where he obtained his BS, MS, and Ph.D. in Medical Microbiology. He worked as a post-doctoral fellow at the Public Health Research Institute (PHRI), Newark, NJ for four years before accepting a three-year faculty position at Brigham Young University-Hawaii. Dr. Engohang-Ndong is a tenured faculty member with the academic rank of Full Professor at Kent State University, Ohio, where he teaches a wide range of biological science courses and pursues his research in medical and environmental microbiology. Recently, he expanded his research interest to epidemiology and biostatistics of chronic diseases in Gabon.",institutionString:"Kent State University",institution:{name:"Kent State University",country:{name:"United States of America"}}},{id:"188773",title:"Prof.",name:"Emmanuel",middleName:null,surname:"Drouet",slug:"emmanuel-drouet",fullName:"Emmanuel Drouet",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/188773/images/system/188773.png",biography:"Emmanuel Drouet, PharmD, is a Professor of Virology at the Faculty of Pharmacy, the University Grenoble-Alpes, France. As a head scientist at the Institute of Structural Biology in Grenoble, Dr. Drouet’s research investigates persisting viruses in humans (RNA and DNA viruses) and the balance with our host immune system. He focuses on these viruses’ effects on humans (both their impact on pathology and their symbiotic relationships in humans). He has an excellent track record in the herpesvirus field, and his group is engaged in clinical research in the field of Epstein-Barr virus diseases. He is the editor of the online Encyclopedia of Environment and he coordinates the Universal Health Coverage education program for the BioHealth Computing Schools of the European Institute of Science.",institutionString:null,institution:{name:"Grenoble Alpes University",country:{name:"France"}}},{id:"131400",title:"Prof.",name:"Alfonso J.",middleName:null,surname:"Rodriguez-Morales",slug:"alfonso-j.-rodriguez-morales",fullName:"Alfonso J. Rodriguez-Morales",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/131400/images/system/131400.png",biography:"Dr. Rodriguez-Morales is an expert in tropical and emerging diseases, particularly zoonotic and vector-borne diseases (especially arboviral diseases). He is the president of the Travel Medicine Committee of the Pan-American Infectious Diseases Association (API), as well as the president of the Colombian Association of Infectious Diseases (ACIN). He is a member of the Committee on Tropical Medicine, Zoonoses, and Travel Medicine of ACIN. He is a vice-president of the Latin American Society for Travel Medicine (SLAMVI) and a Member of the Council of the International Society for Infectious Diseases (ISID). Since 2014, he has been recognized as a Senior Researcher, at the Ministry of Science of Colombia. He is a professor at the Faculty of Medicine of the Fundacion Universitaria Autonoma de las Americas, in Pereira, Risaralda, Colombia. He is an External Professor, Master in Research on Tropical Medicine and International Health, Universitat de Barcelona, Spain. He is also a professor at the Master in Clinical Epidemiology and Biostatistics, Universidad Científica del Sur, Lima, Peru. In 2021 he has been awarded the “Raul Isturiz Award” Medal of the API. Also, in 2021, he was awarded with the “Jose Felix Patiño” Asclepius Staff Medal of the Colombian Medical College, due to his scientific contributions to COVID-19 during the pandemic. He is currently the Editor in Chief of the journal Travel Medicine and Infectious Diseases. His Scopus H index is 47 (Google Scholar H index, 68).",institutionString:"Institución Universitaria Visión de las Américas, Colombia",institution:null},{id:"332819",title:"Dr.",name:"Chukwudi Michael",middleName:"Michael",surname:"Egbuche",slug:"chukwudi-michael-egbuche",fullName:"Chukwudi Michael Egbuche",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/332819/images/14624_n.jpg",biography:"I an Dr. Chukwudi Michael Egbuche. I am a Senior Lecturer in the Department of Parasitology and Entomology, Nnamdi Azikiwe University, Awka.",institutionString:null,institution:{name:"Nnamdi Azikiwe University",country:{name:"Nigeria"}}},{id:"284232",title:"Mr.",name:"Nikunj",middleName:"U",surname:"Tandel",slug:"nikunj-tandel",fullName:"Nikunj Tandel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/284232/images/8275_n.jpg",biography:'Mr. Nikunj Tandel has completed his Master\'s degree in Biotechnology from VIT University, India in the year of 2012. He is having 8 years of research experience especially in the field of malaria epidemiology, immunology, and nanoparticle-based drug delivery system against the infectious diseases, autoimmune disorders and cancer. He has worked for the NIH funded-International Center of Excellence in Malaria Research project "Center for the study of complex malaria in India (CSCMi)" in collaboration with New York University. The preliminary objectives of the study are to understand and develop the evidence-based tools and interventions for the control and prevention of malaria in different sites of the INDIA. Alongside, with the help of next-generation genomics study, the team has studied the antimalarial drug resistance in India. Further, he has extended his research in the development of Humanized mice for the study of liver-stage malaria and identification of molecular marker(s) for the Artemisinin resistance. At present, his research focuses on understanding the role of B cells in the activation of CD8+ T cells in malaria. Received the CSIR-SRF (Senior Research Fellow) award-2018, FIMSA (Federation of Immunological Societies of Asia-Oceania) Travel Bursary award to attend the IUIS-IIS-FIMSA Immunology course-2019',institutionString:"Nirma University",institution:{name:"Nirma University",country:{name:"India"}}},{id:"334383",title:"Ph.D.",name:"Simone",middleName:"Ulrich",surname:"Ulrich Picoli",slug:"simone-ulrich-picoli",fullName:"Simone Ulrich Picoli",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/334383/images/15919_n.jpg",biography:"Graduated in Pharmacy from Universidade Luterana do Brasil (1999), Master in Agricultural and Environmental Microbiology from Federal University of Rio Grande do Sul (2002), Specialization in Clinical Microbiology from Universidade de São Paulo, USP (2007) and PhD in Sciences in Gastroenterology and Hepatology (2012). She is currently an Adjunct Professor at Feevale University in Medicine and Biomedicine courses and a permanent professor of the Academic Master\\'s Degree in Virology. She has experience in the field of Microbiology, with an emphasis on Bacteriology, working mainly on the following topics: bacteriophages, bacterial resistance, clinical microbiology and food microbiology.",institutionString:null,institution:{name:"Universidade Feevale",country:{name:"Brazil"}}},{id:"229220",title:"Dr.",name:"Amjad",middleName:"Islam",surname:"Aqib",slug:"amjad-aqib",fullName:"Amjad Aqib",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/229220/images/system/229220.png",biography:"Dr. Amjad Islam Aqib obtained a DVM and MSc (Hons) from University of Agriculture Faisalabad (UAF), Pakistan, and a PhD from the University of Veterinary and Animal Sciences Lahore, Pakistan. Dr. Aqib joined the Department of Clinical Medicine and Surgery at UAF for one year as an assistant professor where he developed a research laboratory designated for pathogenic bacteria. Since 2018, he has been Assistant Professor/Officer in-charge, Department of Medicine, Manager Research Operations and Development-ORIC, and President One Health Club at Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan. He has nearly 100 publications to his credit. His research interests include epidemiological patterns and molecular analysis of antimicrobial resistance and modulation and vaccine development against animal pathogens of public health concern.",institutionString:"Cholistan University of Veterinary and Animal Sciences",institution:{name:"University of Agriculture Faisalabad",country:{name:"Pakistan"}}},{id:"333753",title:"Dr.",name:"Rais",middleName:null,surname:"Ahmed",slug:"rais-ahmed",fullName:"Rais Ahmed",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/333753/images/20168_n.jpg",biography:null,institutionString:null,institution:{name:"University of Agriculture Faisalabad",country:{name:"Pakistan"}}},{id:"62900",title:"Prof.",name:"Fethi",middleName:null,surname:"Derbel",slug:"fethi-derbel",fullName:"Fethi Derbel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/62900/images/system/62900.jpeg",biography:"Professor Fethi Derbel was born in 1960 in Tunisia. He received his medical degree from the Sousse Faculty of Medicine at Sousse, University of Sousse, Tunisia. He completed his surgical residency in General Surgery at the University Hospital Farhat Hached of Sousse and was a member of the Unit of Liver Transplantation in the University of Rennes, France. He then worked in the Department of Surgery at the Sahloul University Hospital in Sousse. Professor Derbel is presently working at the Clinique les Oliviers, Sousse, Tunisia. His hospital activities are mostly concerned with laparoscopic, colorectal, pancreatic, hepatobiliary, and gastric surgery. He is also very interested in hernia surgery and performs ventral hernia repairs and inguinal hernia repairs. He has been a member of the GREPA and Tunisian Hernia Society (THS). During his residency, he managed patients suffering from diabetic foot, and he was very interested in this pathology. For this reason, he decided to coordinate a book project dealing with the diabetic foot. Professor Derbel has published many articles in journals and collaborates intensively with IntechOpen Access Publisher as an editor.",institutionString:"Clinique les Oliviers",institution:null},{id:"300144",title:"Dr.",name:"Meriem",middleName:null,surname:"Braiki",slug:"meriem-braiki",fullName:"Meriem Braiki",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/300144/images/system/300144.jpg",biography:"Dr. Meriem Braiki is a specialist in pediatric surgeon from Tunisia. She was born in 1985. She received her medical degree from the University of Medicine at Sousse, Tunisia. She achieved her surgical residency training periods in Pediatric Surgery departments at University Hospitals in Monastir, Tunis and France.\r\nShe is currently working at the Pediatric surgery department, Sidi Bouzid Hospital, Tunisia. Her hospital activities are mostly concerned with laparoscopic, parietal, urological and digestive surgery. She has published several articles in diffrent journals.",institutionString:"Sidi Bouzid Regional Hospital",institution:null},{id:"229481",title:"Dr.",name:"Erika M.",middleName:"Martins",surname:"de Carvalho",slug:"erika-m.-de-carvalho",fullName:"Erika M. de Carvalho",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/229481/images/6397_n.jpg",biography:null,institutionString:null,institution:{name:"Oswaldo Cruz Foundation",country:{name:"Brazil"}}},{id:"186537",title:"Prof.",name:"Tonay",middleName:null,surname:"Inceboz",slug:"tonay-inceboz",fullName:"Tonay Inceboz",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/186537/images/system/186537.jfif",biography:"I was graduated from Ege University of Medical Faculty (Turkey) in 1988 and completed his Med. PhD degree in Medical Parasitology at the same university. I became an Associate Professor in 2008 and Professor in 2014. I am currently working as a Professor at the Department of Medical Parasitology at Dokuz Eylul University, Izmir, Turkey.\n\nI have given many lectures, presentations in different academic meetings. I have more than 60 articles in peer-reviewed journals, 18 book chapters, 1 book editorship.\n\nMy research interests are Echinococcus granulosus, Echinococcus multilocularis (diagnosis, life cycle, in vitro and in vivo cultivation), and Trichomonas vaginalis (diagnosis, PCR, and in vitro cultivation).",institutionString:"Dokuz Eylül University",institution:{name:"Dokuz Eylül University",country:{name:"Turkey"}}},{id:"71812",title:"Prof.",name:"Hanem Fathy",middleName:"Fathy",surname:"Khater",slug:"hanem-fathy-khater",fullName:"Hanem Fathy Khater",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/71812/images/1167_n.jpg",biography:"Prof. Khater is a Professor of Parasitology at Benha University, Egypt. She studied for her doctoral degree, at the Department of Entomology, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, USA. She has completed her Ph.D. degrees in Parasitology in Egypt, from where she got the award for “the best scientific Ph.D. dissertation”. She worked at the School of Biological Sciences, Bristol, England, the UK in controlling insects of medical and veterinary importance as a grant from Newton Mosharafa, the British Council. Her research is focused on searching of pesticides against mosquitoes, house flies, lice, green bottle fly, camel nasal botfly, soft and hard ticks, mites, and the diamondback moth as well as control of several parasites using safe and natural materials to avoid drug resistances and environmental contamination.",institutionString:null,institution:{name:"Banha University",country:{name:"Egypt"}}},{id:"99780",title:"Prof.",name:"Omolade",middleName:"Olayinka",surname:"Okwa",slug:"omolade-okwa",fullName:"Omolade Okwa",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/99780/images/system/99780.jpg",biography:"Omolade Olayinka Okwa is presently a Professor of Parasitology at Lagos State University, Nigeria. She has a PhD in Parasitology (1997), an MSc in Cellular Parasitology (1992), and a BSc (Hons) Zoology (1990) all from the University of Ibadan, Nigeria. She teaches parasitology at the undergraduate and postgraduate levels. She was a recipient of a Commonwealth fellowship supported by British Council tenable at the Centre for Entomology and Parasitology (CAEP), Keele University, United Kingdom between 2004 and 2005. She was awarded an Honorary Visiting Research Fellow at the same university from 2005 to 2007. \nShe has been an external examiner to the Department of Veterinary Microbiology and Parasitology, University of Ibadan, MSc programme between 2010 and 2012. She is a member of the Nigerian Society of Experimental Biology (NISEB), Parasitology and Public Health Society of Nigeria (PPSN), Science Association of Nigeria (SAN), Zoological Society of Nigeria (ZSN), and is Vice Chairperson of the Organisation of Women in Science (OWSG), LASU chapter. She served as Head of Department of Zoology and Environmental Biology, Lagos State University from 2007 to 2010 and 2014 to 2016. She is a reviewer for several local and international journals such as Unilag Journal of Science, Libyan Journal of Medicine, Journal of Medicine and Medical Sciences, and Annual Research and Review in Science. \nShe has authored 45 scientific research publications in local and international journals, 8 scientific reviews, 4 books, and 3 book chapters, which includes the books “Malaria Parasites” and “Malaria” which are IntechOpen access publications.",institutionString:"Lagos State University",institution:{name:"Lagos State University",country:{name:"Nigeria"}}},{id:"273100",title:"Dr.",name:"Vijay",middleName:null,surname:"Gayam",slug:"vijay-gayam",fullName:"Vijay Gayam",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/273100/images/system/273100.jpeg",biography:"Dr. Vijay Bhaskar Reddy Gayam is currently practicing as an internist at Interfaith Medical Center in Brooklyn, New York, USA. He is also a Clinical Assistant Professor at the SUNY Downstate University Hospital and Adjunct Professor of Medicine at the American University of Antigua. He is a holder of an M.B.B.S. degree bestowed to him by Osmania Medical College and received his M.D. at Interfaith Medical Center. His career goals thus far have heavily focused on direct patient care, medical education, and clinical research. He currently serves in two leadership capacities; Assistant Program Director of Medicine at Interfaith Medical Center and as a Councilor for the American\r\nFederation for Medical Research. As a true academician and researcher, he has more than 50 papers indexed in international peer-reviewed journals. He has also presented numerous papers in multiple national and international scientific conferences. His areas of research interest include general internal medicine, gastroenterology and hepatology. He serves as an editor, editorial board member and reviewer for multiple international journals. His research on Hepatitis C has been very successful and has led to multiple research awards, including the 'Equity in Prevention and Treatment Award” from the New York Department of Health Viral Hepatitis Symposium (2018) and the 'Presidential Poster Award” awarded to him by the American College of Gastroenterology (2018). He was also awarded 'Outstanding Clinician in General Medicine” by Venus International Foundation for his extensive research expertise and services, perform over and above the standard expected in the advancement of healthcare, patient safety and quality of care.",institutionString:"Interfaith Medical Center",institution:{name:"Interfaith Medical Center",country:{name:"United States of America"}}},{id:"93517",title:"Dr.",name:"Clement",middleName:"Adebajo",surname:"Meseko",slug:"clement-meseko",fullName:"Clement Meseko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/93517/images/system/93517.jpg",biography:"Dr. Clement Meseko obtained DVM and PhD degree in Veterinary Medicine and Virology respectively. He has worked for over 20 years in both private and public sectors including the academia, contributing to knowledge and control of infectious disease. Through the application of epidemiological skill, classical and molecular virological skills, he investigates viruses of economic and public health importance for the mitigation of the negative impact on people, animal and the environment in the context of Onehealth. \r\nDr. Meseko’s field experience on animal and zoonotic diseases and pathogen dynamics at the human-animal interface over the years shaped his carrier in research and scientific inquiries. He has been part of the investigation of Highly Pathogenic Avian Influenza incursions in sub Saharan Africa and monitors swine Influenza (Pandemic influenza Virus) agro-ecology and potential for interspecies transmission. He has authored and reviewed a number of journal articles and book chapters.",institutionString:"National Veterinary Research Institute",institution:{name:"National Veterinary Research Institute",country:{name:"Nigeria"}}},{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",country:{name:"India"}}},{id:"94928",title:"Dr.",name:"Takuo",middleName:null,surname:"Mizukami",slug:"takuo-mizukami",fullName:"Takuo Mizukami",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94928/images/6402_n.jpg",biography:null,institutionString:null,institution:{name:"National Institute of Infectious Diseases",country:{name:"Japan"}}},{id:"233433",title:"Dr.",name:"Yulia",middleName:null,surname:"Desheva",slug:"yulia-desheva",fullName:"Yulia Desheva",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/233433/images/system/233433.png",biography:"Dr. Yulia Desheva is a leading researcher at the Institute of Experimental Medicine, St. Petersburg, Russia. 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We encourage the submission of manuscripts that provide novel and mechanistic insights that report significant advances in the fields. Topics can include but are not limited to: Biotechnology such as biotechnological products and process engineering; Biotechnologically relevant enzymes and proteins; Bioenergy and biofuels; Applied genetics and molecular biotechnology; Genomics, transcriptomics, proteomics; Applied microbial and cell physiology; Environmental biotechnology; Methods and protocols. Moreover, topics in biosensor technology, like sensors that incorporate enzymes, antibodies, nucleic acids, whole cells, tissues and organelles, and other biological or biologically inspired components will be considered, and topics exploring transducers, including those based on electrochemical and optical piezoelectric, thermal, magnetic, and micromechanical elements. Chapters exploring biomaterial approaches such as polymer synthesis and characterization, drug and gene vector design, biocompatibility, immunology and toxicology, and self-assembly at the nanoscale, are welcome. Finally, the tissue engineering subcategory will support topics such as the fundamentals of stem cells and progenitor cells and their proliferation, differentiation, bioreactors for three-dimensional culture and studies of phenotypic changes, stem and progenitor cells, both short and long term, ex vivo and in vivo implantation both in preclinical models and also in clinical trials.",annualVolume:11405,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/9.jpg",editor:{id:"126286",title:"Dr.",name:"Luis",middleName:"Jesús",surname:"Villarreal-Gómez",fullName:"Luis Villarreal-Gómez",profilePictureURL:"https://mts.intechopen.com/storage/users/126286/images/system/126286.jpg",institutionString:null,institution:{name:"Autonomous University of Baja California",institutionURL:null,country:{name:"Mexico"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"35539",title:"Dr.",name:"Cecilia",middleName:null,surname:"Cristea",fullName:"Cecilia Cristea",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYQ65QAG/Profile_Picture_1621007741527",institutionString:null,institution:{name:"Iuliu Hațieganu University of Medicine and Pharmacy",institutionURL:null,country:{name:"Romania"}}},{id:"40735",title:"Dr.",name:"Gil",middleName:"Alberto Batista",surname:"Gonçalves",fullName:"Gil Gonçalves",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYRLGQA4/Profile_Picture_1628492612759",institutionString:null,institution:{name:"University of Aveiro",institutionURL:null,country:{name:"Portugal"}}},{id:"211725",title:"Associate Prof.",name:"Johann F.",middleName:null,surname:"Osma",fullName:"Johann F. 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