Main proteomic technologies applied for intracellular mechanisms investigation in skin cancer.
This chapter describes the main deregulated intracellular pathways at both genetic and proteomic levels that are found in three main skin cancers: basal cell carcinoma, squamous cell carcinoma and melanoma. In basal cell carcinoma, the main intracellular signaling pathways is the Sonic Hedgehog pathway, while in squamous cell carcinoma, it is the p53 pathway. However, in both nonmelanoma skin cancers, these major pathways trigger cross-activation with other important ones. In melanoma, mitogen-activated protein kinase pathway and PI3K/Akt pathways are deeply deregulated, and moreover due to the disease complexity, BRAF, RAS (N/H/K), NF1 and Triple-WT melanoma subtypes need additional molecular stratification. The stage in which photodynamic therapies’ clinical application is in the treatment of these diseases is another subject tackled by the chapter. Thus, if basal cell carcinoma and squamous cell carcinoma possess in their therapeutical armamentarium photodynamic therapies approach, melanoma, with its particularities, still needs thorough molecular investigations to adapt this particular therapy. Based on the accumulated knowledge on pathological intracellular pathways, the chapter describes the molecular details that reside in applying photodynamic therapy. In vivo and in vitro models of cutaneous malignancy and photodynamic therapies’ molecular events are further detailed.
- skin cancer
- intracellular signaling
- photodynamic therapy
Skin cancers, especially cutaneous melanoma, remain a complex therapeutic challenge owing to a multiangle problem such as the emergent incidence in white population, the inefficiency of classical therapies like surgery and owing to transition to the new wave treatments like targeted therapies, immunotherapy and alternative therapies like photodynamic therapy (PDT). Skin tumors are classified as melanoma and nonmelanoma type. Basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) along with actinic keratosis (AK) are considered nonmelanoma skin cancers (NMSC), being the most frequent skin cancers in comparison with melanoma which is more rare (only 4%) but highly deadly . Similarly, organ transplant recipients (OTR) record an extremely high risk of developing NMSC . All these types of cancers are generated by complex molecular events that favor tumor proliferation and invasion. At the core of this diseases’ pathology intracellular pathways deregulation dwell. Therapies that are developed in each cancer type should acknowledge the molecular events particularities. In the therapeutical armamentarium of skin cancer, PDT has already gained its place. As further presented, the intracellular molecular pattern triggered in each type of skin cancer by PDT has both common outlines and particularities.
2. Skin cancer, photodynamic therapy and signaling pathways
2.1. Biology of skin cancer in the light of new therapeutical era
Deepening the biology of skin cancer by unraveling the intracellular mechanisms that trigger the neoplastic transformation could lead to deciphering new therapy targets and new therapeutical approaches. Both cellular and molecular basis of a successful therapy still needs new explorations and additional biomedical technologies in order to manage this high morbidity and mortality group of diseases.
2.1.1. Basal cell carcinoma
As described in the early 1990 , basal cell carcinoma (BCC) is the most common malignancy in humans, and although rarely metastatic, accounts for about two-thirds of all skin cancers with a worldwide incidence steady increase . The diversity in the phenotypic appearance of BCCs relies on the fact that the majority of BCCs represent monoclonal tumors and anatomically distinct BCCs may sometimes share the same cellular origin . An extensive genetic study on BCC profiling was published in 2016 investigating around 300 tumor tissue that displayed the highest mutation rate in cancer (65 mutations per mega base). About 85% of all tumors have mutations in the Sonic Hedgehog (Hh) pathway. These genes are PTCH1, SMO, SUFU and TP53. Other mutations were found in MYCN, PPP6C, STK19, LATS1, ERBB2, PIK3CA and NRAS. Loss-of-function and deleterious missense mutations were found in PTPN14, RB1 and FBXW7 genes . Moreover, 2016 studies have shown that genetic predisposition in familial BCC has the most commonly gene mutated, PTCH1, main player in the Hh pathway. Another gene associated with familial BCC is SUFU being involved in the same pathway. This gene is loosing its function and hence inducing BCC predisposition. Understanding the deregulated genes that trigger BCCs can lead to new targeted therapy trials . In the last five years, studies on the biology of BCC have shown that Hh pathway is deeply involved in the initiation of this skin tumor. This pathway cross-talks with other main intracellular pathways involved in skin’s homeostasis. Hence, Wnt pathway was found as having increased levels of beta-catenin, a critical mediator of Wnt signaling in BCCs [8, 9]. Another important pathway involved in BCC is EGFR/MEK/ERK that modulates GLI-dependent transcription in human keratinocytes and drives their oncogenic transformation [10, 11]. As epithelial-stromal interactions are creating a protumoral microenvironment, stromal cells isolated from BCCs, have high levels of gremlin 1. This protein antagonizes with the prodifferentiation factors BMP2 and BMP4, sustaining, therefore, tumor proliferation . If Hh pathway is deregulated at gene and protein level, another deregulated pathway in BCC is the MEK-ERK pathway. Hence, acknowledging that IL-17 (IL-17A) sustains a chronic inflammatory microenvironment with protumoral consequences some important reports were published in 2015. IL-17 binding to its receptor activates the route IL-17R-Act1-TRAF4-MEKK3-ERK5 that directly stimulates keratinocyte proliferation and tumor formation. In the BCC context, this axis sustains the expression of Steap4-p63 through p63-mediated TRAF4 expression that directly enhances keratinocyte proliferation and further tumor formation .
2.1.2. Squamous cell carcinoma
Cutaneous squamous cell carcinoma (SCC) is the second most common human skin malignancy after BCC, and opposed to BCC, it can trigger metastasis. SCC originates the premalign lesions actinic keratosis (AK) and it develops from keratinocytes of the spinous layer. The progression of AK to malignancy relies on the sequential DNA mutations in oncogenes and tumor suppressor genes. This multistep process triggered by chronic UV irradiation leads to increased genomic instability and loss of cell cycle control, thus driving the malignant uncontrolled proliferation of keratinocytes. Details of the molecular pathogenesis of SCC are still a subject of intense research . Intracellular signaling deregulated pathways in SCC pinpoint to p53 pathway as mutated p53 is the most common genetic abnormality found in SCC. AK lesions can harbor mutated p53, and remain as such through the malignization process toward SCC [15, 16]. Whole transcriptome analysis published in 2016 has shown in SCC cancer cells, in comparison to normal human epidermal keratinocytes, an overexpression of long intergenic non coding RNA (LINC00162). LINC00162 is upregulated by the inhibition of the p38α and p38δ mitogen-activated protein kinases. Knockdown of LINC00162 inhibited proliferation and migration of SCC cells
2.1.3. Cutaneous melanoma
Cutaneous melanoma is one of the solid tumors that bear the highest rate of mutations. In 2015, The Cancer Genome Atlas Network has proposed a new genomic classification of these tumors divided into genomic subtypes: BRAF, RAS (N/H/K), NF1 and Triple-WT . These subtypes have significant intracellular pathways deregulations. A BRAF mutation is present in the majority of melanomas, and an NRAS isoform is present in 15–30% of melanomas . The mitogen-activated protein kinase (MAPK) pathway (Ras/Raf/MEK/ERK pathway) has several mutated points so that uncontrolled cellular proliferation may occur [23, 24]. Alike to the MAPK pathway, the PI3K/Akt pathway can also be activated by Ras. Once this pathway is activated, cell proliferation and invasion are promoted. Although PI3K mutations are believed to be rare, downstream components of the PI3K/Akt pathway steadily increase during melanoma progression, and are altered in 50–60% of melanomas . CDKN2A is another gene that encodes proteins involved in cell cycle regulation. Approximately 10% of all melanomas have a familial susceptibility linked to the CDKN2A gene. Somatic CDKN2A mutations have been reported in 30–70% of sporadic melanomas .
The pathophysiology of each skin cancer can have different gene/protein/intracellular signaling foundation or can share the same molecular pathways. Figure 1 resumes the main intracellular pathways that trigger the three main skin cancers in humans.
2.2. PDT in the clinical management of skin cancers
Owing to a constantly increasing incidence, malignant skin tumors need a multidisciplinary approach regarding their clinical management, comprising specialists and therapeutical lines, which could be personalized for every situation . In this context, PDT is involved in the management of nonmelanoma skin cancers, primary superficial BCCs, low-risk nodular BCCs and superficial SCCs . In recent years, even for melanoma, PDT starts to be considered as an alternative treatment option.
2.2.1. PDT in BCC therapy
Predominantly located in head and neck region, there are three types of BCC: nodular, superficial and morpheic with an increased heterogeneity [28, 29]. Due to this heterogeneity, there are several therapy lines and several ongoing clinical trials that are thoroughly resumed in Ref. . Among all these therapies, PDT gains its place. Thus, topical PDT with methyl ester-based photosensitizer (PS) is currently accepted for superficial BCC. Accordingly, 16% methyl ester methyl aminolevulinate (MAL) is approved for topical PDT of BCC in Europe (Metvix®) while in the USA the corresponding approved drug is Levulan® (20% of 5-aminolevulinic acid in ethanol solution) . The clinical response for PDT in BCC is not clear-cut. Thus evaluating more than 130 patients treated with MAL-PDT the best response was obtained for superficial BCC (82%) compared to nodular BCC (only 33%). Analyzing the subtypes some predictor factors emerged, nodular infiltrative histotypes, ulceration tumor thickness and localization on limbs were the negative ones . Trying to improve the clinical outcome, two-fold illumination scheme for aminolevulinic acid-photodynamic therapy (ALA-PDT) was investigated and one-year post-treatment clinical evolution was assessed. For small BCC located outside the head and neck area, this activation scheme has proven good clinical outcome . For a three-year follow-up in patients treated with MAL-PDT, fluorouracil and MAL-PDT have proven the same efficacy in the treatment of superficial BCCs .
2.2.2. PDT in SCC therapy
SCC and AK represent the same skin disease but in different stages of evolution, as AK is superficial, SCC involves also the dermis. Local PDT is suitable for AK and for
2.2.3. PDT in melanoma therapy
There is an interesting link between melanoma patients and the subsequent appearance of BCC or SCC. In a study published in 2016, the associations between melanoma diagnosis and SCC were studied. The study showed a clear correlation between age, sex, skin characteristics, sun exposure and the existence of p.R163Q/p.D294H MC1R variants in melanoma patients, parameters that favor the risk of developing a SCC. This study has shown that melanoma patients with increased risk of developing another skin cancer should be further stratified .
Although in cutaneous melanoma targeted immune-therapies are the best clinical option, there is a therapeutical niche for PDT with second generation PS, especially as postsurgery adjuvant treatment or even as a preventive approach . PDT exerts specific effects in melanoma cells both upon melanocytic antioxidant system and multidrug resistance cellular machinery decreasing these functions essential for melanoma survival. While successful in other skin cancers, PDT is not effective in pigmented melanoma due to photophysical properties of melanin from melanocyte-transformed cells, as melanin absorbs light over the entire wavelength region used by PS for PDT (400–750 nm). Amelanotic melanoma with low melanin pigment load has been more approached in PDT studies . Still only few studies are reported in this topic, and clinically there are some promising results for PDT with chlorin e(6) in skin metastases  and ocular choroidal melanomas [40, 41]. PDT with verteporfin alone or in combination with bevacizumab may be useful as primary or preoperative procedure for ocular melanoma. There are also recent experimental studies involving amelanotic melanoma, the nonpigmented melanoma type owned to a poor differentiation of melanocytes, which produce less melanin, refractive to classic treatments and for which alternative therapies are taking into account . Thus, in a mouse C57/BL6 model bearing B78H1 amelanotic melanoma, it was tested a novel PS assembly comprising a Zn(II)-phthalocyanine, a polyethylene glycol (PEG) derivative and gold nanoparticles. The deposition of PEG on the nanoparticle surface makes the conjugate hydrosoluble prolonging the retaining in serum, improving thus the PDT efficacy. The nanoparticle conjugates were significantly accumulated and retained in the tumor 3 h postinjection followed by PDT. The experimental approach lead to 40% survival of the treated mice, without tumor relapses. These types of PS functionalized with nanoparticles have good potential in PDT for difficult to treat cancers such as amelanotic melanoma . Another strategy is to use natural compounds as PS in PDT of skin cancer; hence, positive results were obtained with hypericin in human melanoma cells, Where an inhibition of proliferation was registered . In addition, natural compounds are “back in fashion” and hypericin was tested also in NMSC treatment approaches.
2.3. Specific intracellular networks triggered by PDT in skin tumor cells
PDT is an alternative therapy for some type of cancers and several nonmalignant diseases, involving application and preferentially accumulation of a PS in the target cell/tissue, followed by PS photoactivation with a light wavelength fitting the compound’s absorption. The actual effect of PDT depends on many factors but the cell/tissue where the PS is accumulating triggers the main process (Figure 2).
Typically the structure of many PSs is based on the tetrapyrrole ring—porphyrins and phthalocyanines, including their derivatives (porphycenes)—or natural products such as hypericin, riboflavin or curcumin . Several conditions should be attained by a potential PS, namely: chemical purity, stability, high solubility in water, preferentially loading in the target cell/tissue, low “dark” toxicity and high quantum yield of singlet oxygen (1O2) generated upon photoactivation . This light activation causes the transition of PS first in a short-lived excited state then in a long-lived energetic state triggering reactive oxygen species (ROS) as main agents of tumor vasculature damage, immune response generation and further tumor eradication [46, 47]. Among relatively innovative approaches in skin tumors treatment, especially in NMSC, PDT could reveal new lines in terms of novel PSs embossing different insights in key intracellular pathways influenced by the PDT treatment. Elucidation of mechanisms in PDT has gained advance within the last decade, data regarding signaling pathways, transcription factors related to cell cycle control, inflammation and cellular death accumulated lately. Nevertheless in a living cell, all these processes are deeply overlapped so minute understanding should be proceeded step by step . Furthermore, survival mechanisms are activated in PDT-treated cells and some transcription factors have been identified to be involved in cell resistance following PDT, such as AP-1 transcription factor family members, NRF2, hypoxia-inducible factor-1 alpha (HIF-1), nuclear factor-kappa B (NF-κB), HSF1 and unfolded protein response (UPR) protein group . Involvement of a certain signaling pathway could define the response of a treatment. For instance PDT with ALA as PS has been widely used to treat SCC. However, a segment of SCC patients does not respond well to this PDT therapy, the lack of efficacy being evoked at molecular level by the MAPK signaling pathway.
Figure 3 resumes intracellular events triggered by PDT in cells that were uptaking PS.
2.3.1. Pathways in intracellular events triggered by various photosensitizers used in PDT
As a general picture, in skin cancers, the PDT is a topic procedure, especially applied for NMSC while for melanoma could be an adjuvant postsurgery alternative. As PS used in dermatopathology, only two of them are known to be approved by FDA, namely 5-ALA and MAL, which are prodrugs becoming active upon intracellular metabolization , while other PS attempts are currently made with novel (bio) structures related to porphyrin skeleton such as porphycenes [51, 52]. Once activated in tumor cell, a PS triggers different pathways depending on intracellular localization of PS, PS’s dose and type, light dose, cell genotype, affecting cell fate in terms of death and proliferation. PDT triggers the death of cellular target, occurred primarily by apoptosis, necrosis and, as recently shown, by autophagy . The PS generations evolve continuously, hence from the first generation of PS, Photofrin, a partially purified form of hematoporphyrin derivative , the second generation was developed to overcome disadvantages of the first one, such as tetrapyrrole rings, substituted derivatives of porphyrin, chlorin and bacteriochlorin . Recently the newer, third generation of PSs was put in scene in novel chemical compounds (e.g. fullerenes) or novel platforms such as PS coupled on different carriers . Nowadays, a so called targeted PDT has emerged and in this type of therapy, antibodies, peptides, proteins, liposomes, cholesterol or other ligands are coupled to PS displaying an improved selective accumulation in the tumor .
An advanced generation of PS is always defined by longer wavelengths of light (as red as possible) which corroborates with a deeper penetration of target tissues and a decreased photosensitivity . The antitumor PDT addresses two important issues: direct harmful effects on target cells and vascular injury that will limit blood and other nutrients supply to the affected region . There are mainly two ways of cell fate following PDT: cellular death or cellular survival. The border between these two opposite processes is fine-lined by specific intracellular mechanisms development.
18.104.22.168. Cellular death induced by PDT
Once inside the target cell subjected to photoactivation, a variety of intracellular pathways are initiated by the PS. Although at first sight these biochemical pathways look complex, overlapped, multiple and hard to decipher, the target cell will act in one main direction, namely survival as a response to PDT-triggered aggression.
What is the fate of ROS in photosensitized cells? Different types of ROS inflict different actions. Singlet oxygen being highly reactive but evanescent, will oxidize various many biomolecules (lipids, nucleic acids, proteins, etc.) at the level of their electron-dense regions [57, 60], while superoxide anion due to its low reactivity on direct biological target, will act mainly as a precursor for other reactive species (e.g. H2O2 or •OH) that will cause cellular fatal injuries triggering cell death through necrosis or apoptosis . Also it is important to notice that since singlet oxygen is a short-lived species, will act immediately upon intracellular targets close to the site where PS was accumulated, influencing thus the type of response upon intracellular localization . Regardless of ROS type, the outcome is tumor cell eradication by different mechanisms of cytotoxicity operated by PDT .
22.214.171.124. Activation of intracellular survival pathways in photodynamic action
Although cellular death conducted by ROS is envisaged, tumor cells subjected to PDT could encounter this stress by triggering survival mechanisms when vascular shutdown was not completed following PDT action. This type of response is primarily mediated by several pathways raising in an interconnected manner where beside classical NF-kB-mediated proinflammatory and proangiogenic activity, is raising also a NRF2-mediated antioxidant response, a HIF-1-mediated hypoxia survival, a proteotoxic stress response interceded by certain transcription factors (HSF1, XBP1, ATF6 and ATF4) corroborated with an acute stress reply where factors from MAPK pathway are being involved. As PDT means an oxidative stress upon target cells, many studies related to signaling pathways were treated through the prism of oxidative stress and therefore extrapolated to PDT. Consequently, recent works refer to signaling pathways in PDT as to signaling pathways activated in cells subjected to oxidative stress .
NRF2 is the main transcription factor protecting against the oxidative stress by restoring the intracellular redox balance in a post-PDT–treated cell, promoting the transcription of the genes encoding for antioxidant enzymes, antioxidant proteins as well as for multidrug response proteins. Moreover, NRF2 is likely constitutively active in many cancer types potentially desensitizing these cells to PDT effects, mediated by ROS. Cells from various layers of the skin benefit from NRF2 protective actions, both in abnormal differentiation, wound healing and controlling inflammation. Thus in keratinocytes and melanocytes, NRF2 protects against mutation during keratinization and melanogenesis. Also, in fibroblasts, NRF2 protects against differentiation and fibrosis processes; therefore, NRF2 activity could be modulated in the context of skin diseases pharmacotherapy and in PDT in order to improve the PDT efficacy by impairing adaptation of target cells to oxidative stress. In addition, NRF2 could be a key biomolecule in the searching for new drugs for various skin disorders including vitiligo or even cutaneous melanoma .
NF-κB is a family of transcription factors with a crucial role in inflammation, apoptosis, innate immunity and also in cancer initiation. NF-kB interferes with an ample array of signaling pathways, including HIF-1, and certain biomolecules such as ROS . The NF-κB activation following PDT could initiate the survival of tumor cells by inhibiting apoptosis and facilitating angiogenesis. Also, NF-κB pathway could display equally antitumor and protumor functions in different carcinogenesis processes, for instance in epidermal keratinocytes, NF-κB seems to exert mainly tumor growth inhibitory functions . In melanoma, the NF-κB activity in tumorigenesis was demonstrated in a mouse model where HR as-mediated tumorigenesis onset relies on IKK2-mediated NF-κB activation .
HIF-1 is the central modulating pathway for hypoxic conditions in most tumor cells, as well as skin cancers, condition in which it is constitutively activated . Thus, in a hypoxic or even anoxic milieu, HIF-1 becomes hyperactivate as a part of survival actions adopted post-PDT .
The ASK1 pathway directs the immediate early stress response, namely the rapid transcription of a set of genes encoding for stress adapting proteins. Classical ASK1 sends its signal
The proteotoxic stress response arises as well as a survival mechanism in PDT stressed cells triggering certain transcriptional level responses known as the unfolded protein response (UPR), a form of endoplasmic reticulum (ER) stress. These resulted unfolded proteins activate further HSF transcription factors by which an adaptive response comprising chaperones upregulated and protein synthesis inhibition is raising, allowing protein refolding and degradation of those protein aggregates wrongly appeared during stress .
However, this UPR process should be analyzed in report with cell, type, PS nature, PS intracellular localization and PDT regimen. It is expected to launch an UPR response for those PS which accumulate preferentially in ER, such as hypericin. For instance, such ER-related oxidative stress was reported for a PDT model with sodium-porfimer photosensitizer, which conducts to protein polyubiquitination, carbonylation and ER lumen enlargement . As a result, the UPR constitutively activated in tumors, as many other protection mechanisms , will protect tumor cells against anticancer therapies such as PDT .
2.3.2. Different effects triggered by in vitro PDT experimental models
Subtle changes triggered by photodynamic treatment are incompletely revealed, as signaling pathways regulate a myriad of cellular processes starting with genetic ones, such as transcription and translation, and ending with complex cellular behavior such as proliferation, apoptosis, differentiation, metabolism and overall cell survival upon a certain therapeutic regimen. Such finest inquiries are needed to be explored with various techniques and experimental models that deliver data regarding the best way of PS delivery, the best PDT regimen, cellular and molecular characteristics imprinted by a certain PS, therapeutical outcome and so on. The “practical” history of experimental PDT begun in the mid-1970s when it was discovered that a hematoporphyrin derivative activated with red light “cured” a mammary tumor in a mouse model . The studies are in continuous development as an arsenal of in vitro cellular models were settled for studying PDT effects on various cancers including skin cancers. Experimental
126.96.36.199. In vitro models for nonmelanoma skin cancer PDT therapy
Among PS tested for NMSC, hypericin a natural quinone extracted from
188.8.131.52. In vitro models for melanoma PDT therapy
The main stream of the studies regarding PDT applied in melanoma encounter a major issue raised by this type of cancer: an evident resistance to this therapy owing to melanin that will compete with the PS for photons in the detriment of molecular oxygen, leading to an impaired phototoxicity upon target cell . Therefore, the attempts regarding PDT in malignant melanoma have tried to combine PDT with a complementary procedure such as magneto-hyperthermia , or use an improved PS delivery system, namely a liposome formula including a second generation PS such as metallated phthalocyanines. This
Although PDT seems not to be an option in melanoma, recent publication has shown that in a B16-melanoma cell line and also in a B16 ectopic tumor model, ALA-SDT had been more efficacious when compared to ALA-PDT. SDT is sonodynamic therapy in which the activation of a nontoxic sensitizer drug is performed using low-intensity ultrasound to produce cytotoxic ROS. SDT can activate sensitizers at a greater depth within human tissue because of the low tissue attenuation of ultrasound. In PDT for melanoma the low efficacy was attributed to the dark pigmentation of the melanoma that filters the excitation light. In SDT, the sensitizer is activated by ultrasound and it is not hindered by pigmentation. These results suggest SDT as a better approach in comparison to PDT when treating highly pigmented cancerous skin lesions .
Nanocarriers for delivering one or even simultaneously two PSs  seem to be a good instrument to outcome the recognized melanoma resistance, and have been tested further in a mouse model of xenograft melanoma proving an increased efficacy of treatment and an enhanced accumulation in melanoma cell .
2.4. Proteomics technologies in intracellular signaling events triggered by PDT
The complex and intermingled intracellular mechanisms triggered by PDT claim high-throughput proteomic tools to thoroughly quest the signaling events occurred in cancer cell followed PS activation. The cellular signaling events are first triggered by the activation of plasma membrane events . Recently emerged, the proteomics branch focusing on these events is plasma membrane proteomics. From antibody-based techniques to large-scale “precision proteomics” centered on mass spectrometry, posttranslational modifications, protein-protein interactions and changes in protein expression could be analyzed by large scale proteomics. Proteomics in this domain is important as it conveys accurate information concerning (patho) physiological changes in terms of qualitative and quantitative terms of thousands of proteins as response to a certain antitumor therapy.
Skin cancers alterations affect specific genes and thus specific protein mediators from different signaling pathways including the Sonic Hedgehog and NF-kB, targeting these proteins being the trigger for new approaches in skin cancers therapy .
Before tackling the proteomic involvement in PDT pattern, it must be underlined that genomic technologies’ advancements paved the road to molecular insights in skin cancers and related signaling pathways. In skin tumors, advances in sequencing techniques were driven at the beginning of 2000s in receptor tyrosine kinases studies, and were the tools that identified and indicated the presence of BRAF mutations in 50% of skin melanomas . This imprints a major impact in development of selective BRAF inhibitors (vemurafenib and dabrafenib) triggering impressive remissions in melanoma patients who benefit now from an improved treatment, leading to a new era in targeted therapy . Starting from this crossing-point, it could be further underlined that proteomics is continuing to make major progresses in biological processes discoveries as well as in establishing an “universal” assay platform for measuring proteins status and levels in any biological system subjected to different physiological milieu. A strength of proteomic approach is that it can translate almost in “real-time” fundamental science achievements into clinical practice helping in outlining personalized medicine and precision medicine .
2.4.1. Proteomic technologies in skin cancers at a glance
Proteomics includes a number of methods that could be classified depending on several criteria, such as scope (for identification—quality
It is not the chapters’ intend to go in depth with proteomic techniques but one should keep in mind that there are several main stream approaches currently in use in different experimental sets in cancer research [97, 99] as resumed in Table 1.
|Two-dimensional gel electrophoresis (2-DE)||Quantitative method allowing extraction and separation in two dimensions (isoelectric point, molecular mass) of proteins from sample of interest|
|Mass spectroscopy (MS)||Generates peptide mass fingerprints for protein detected on 2-DE; MS has multiple variants—electrospray ionization-liquid chromatography tandem mass spectroscopy (ESI-LC-MS); matrix-assisted laser desorption ionization time of flight (MALDI-TOF); surface-enhanced laser desorption ionization time of flight (SELDI-TOF); MALDI MS imaging (MALDI-MSI); laser capture microdissection-MS (LCM-MS)—optimum for extracting cells from biological specimens preserving the morphologies of captured cells and the nearby tissues|
|Protein microarray (PM) technology||Proteins/antibodies/other biomolecules covalently attached to a solid support like glass are used to detect various interactions such as protein-protein, enzymatic targets, protein-small molecule (peptide, DNA, etc.), based on the antigen-antibody reaction; it has also many recent variants—cell arrays—it can analyze particular molecular targets expression triggered in in vivo experimental models; tissues array—it can analyze the molecular targets in situ across a panel of primary tissues in order to evaluate their clinical significance|
The future in the proteomic domain relies in multianalyte investigation with different congruent methods based on molecular characteristic evaluation such as 2-DE, MS and protein microarrays. As the proteomic approach is complex, so the future therapeutical approaches in skin cancer need the same multitargeted approach. In this aspect, photo-immune-theranostics reagents are the future compounds that will enter the PDT scene. This future to be therapeutical method combines molecular optical imaging, photodynamic therapy and immunotherapy using SNAP-tag technology which is a derivative of the O(6)-alkylguanine-DNA alkyltransferase (AGT) with the ability to efficiently conjugate to O(6)-benzylguanine (BG) molecules under physiological conditions depending on its folding pattern. An approach like this could simultaneously monitor and suppress the growth of skin squamous carcinoma and melanoma cells expressing EGFR .
2.4.2. Proteomic data for skin cancers
Data for cutaneous tumors were obtained from proteomic studies’ involving MS. Referring strictly to cutaneous cancers, ESI-LC-MS was used for investigating in paraffin-embedded metastatic melanomas for comparative proteomic study . This method quantifies peptide spectra that have been sequenced by the MS and can be used for biomarkers discovery through comparing the peak intensities derived from multiple LC-MS data set .
Biomarker identification in melanoma along with other type of cancers was also subjected of SELDI-TOF-MS analysis . In SELDI, the protein sample mixture is spotted on a specific solid surface with chemical functionality such as binding affinity where some analytes in the samples would attach while the others will be washed off. The spotted samples on an SELDI surface are analyzed with TOF mass spectrometry .
Nevertheless, proteomic studies concerning PDT in skin tumor are still missing and are limited to
PM methodology is not yet a routine approach in PDT topic, although possess all strengths to become a robust tool in deciphering the protein-pattern of this domain. Many formats have been developed with whole proteomes, peptides, nucleic acids and lectins, although antibodies platforms remain the most popular PM surfaces. High-throughput tools in a miniaturized format, the arrays could perform parallel analysis, interactions and protein function on a large scale for benefit of both basic research and clinical applications .
Using antibody microarray, we have assessed the probable intracellular pathways by which PDT with aluminum-substituted disulfonated phthalocyanine trigger apoptosis in dysplastic oral keratinocytes cells (DOK cell line), leading to the tumoral cells eradication. Among the analyzed apoptotic factors, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins were the biomolecules whose expression changes with the greatest amplitude. Until now, the intimate apoptotic mechanisms activated by PDT with metallated phthalocyanine in this type of keratinocytes are still to be deciphered as well as PDT-related signaling events per se . This complex methodology is a versatile tool allowing investigation in detail of molecular events related to cellular death induced by PDT.
PDT procedures have several lines of improvement in skin cancers treatment. For example, BCC superficial lesions, preferentially located on the trunk, have the best therapeutic response when treated with PDT. In SCC, PDT should be combined with immune modulators and chemotherapeutic agents. For melanoma, there is still a huge array of improvement due to its particularities and probably with the prospect of advances in gene discovery and translation, multidisciplinary team has to solve all the emerging issues for introducing PDT in melanoma.
The therapeutic future relies in the homogeneous photo-immune-theranostics reagents combining molecular imaging, PDT and immunotherapy. Using “next generation” proteomic technologies (SNAP-tag) it would be possible to simultaneously monitor and suppress the growth of skin squamous carcinoma and melanoma cells expressing specific markers, like EGFR.
Concluding at a glance, large-scale proteomics-based signaling research will be one of the leaders in future photomedicine by enlarging the basic knowledge regarding (photo)therapy-targeted networks and molecules, and by deciphering new intracellular avenues for future precision medicine in skin tumors. A deeper knowledge regarding signaling mechanisms in PDT could furnish new molecular targets and increase its clinical efficacy.
This work was financed by national grants PNII-ID-PCE-2011-3-0918 and PNII-PT-PCCA-2013-4-1407.
Neagu M. The immune system—a hidden treasure for biomarker discovery in cutaneous melanoma. Adv Clin Chem. 2012;58:89–140. DOI: 10.1016/B978-0-12-394383-5.00011-4
Bouwes Bavinck JN, Euvrard S, Naldi L, Nindl I, Proby CM, Neale R, et al. Keratotic skin lesions and other risk factors are associated with skin cancer in organ-transplant recipients: a case-control study in The Netherlands, United Kingdom, Germany, France, and Italy. J Invest Dermatol. 2007;127(7):1647–1656. DOI: 10.1038/sj.jid.5700776
Miller SJ. Biology of basal cell carcinoma (Part I). J Am Acad Dermatol. 1991;24(1):1–13002E
Wu L, Jemal A, Siegel R eds. American Cancer Society. Cancer Facts and Figures. 2011. Atlanta, Georgia, USA: American Cancer Society, Inc.; 60 p.
Shulman O, Laitman Y, Vilan A, Leviav A, Friedman E. Monoclonal origin of anatomically distinct basal cell carcinomas. J Invest Dermatol. 2006;126(3):676–679.
Bonilla X, Parmentier L, King B, Bezrukov F, Kaya G, Zoete V, et al. Genomic analysis identifies new drivers and progression pathways in skin basal cell carcinoma. Nat Genet. 2016;48(4):398–406. DOI: 10.1038/ng.3525
Shanley S, McCormack C. Diagnosis and management of hereditary basal cell skin cancer.Recent Results Cancer Res. 2016;205:191–212. DOI: 10.1007/978-3-319-29998-3_11
Adolphe C, Hetherington R, Ellis T, Wainwright B. Patched1 functions as a gatekeeper by promoting cell cycle progression. Cancer Res. 2006;66(4):2081–2088. DOI: 10.1158/0008-5472.CAN-05-2146
Yang SH, Andl T, Grachtchouk V, Wang A, Liu J, Syu LJ, et al. Pathological responses to oncogenic Hedgehog signaling in skin are dependent on canonical Wnt/beta3-catenin signaling. Nat Genet. 2008;40(9):1130–1135. DOI: 10.1038/ng.192
Kasper M, Schnidar H, Neill GW, Hanneder M, Klingler S, Blaas L et al. Selective modulation of Hedgehog/GLI target gene expression by epidermal growth factor signaling in human keratinocytes. Mol Cell Biol. 2006;26(16):6283–6298. DOI: 10.1128/MCB.02317-05
Schnidar H, Eberl M, Klingler S, Mangelberger D, Kasper M, Hauser-Kronberger C, et al. Epidermal growth factor receptor signaling synergizes with Hedgehog/GLI in oncogenic transformation via activation of the MEK/ERK/JUN pathway. Cancer Res. 2009;69(4):1284–1292. DOI: 10.1158/0008-5472.CAN-08-2331
Sneddon JB, Zhen HH, Montgomery K, van de Rijn M, Tward AD, West R, et al. Bone morphogenetic protein antagonist gremlin 1 is widely expressed by cancer associated stromal cells and can promote tumor cell proliferation. Proc Natl Acad Sci USA. 2006; 103(40):14842–14847. DOI: 10.1073/pnas.0606857103
Chen X, Zhao J, Martin B, Zepp JA, Ko JS, Gu C, et al.A novel IL-17 signaling pathway controlling keratinocyte proliferation and tumorigenesis via the TRAF4-ERK5 axis. J Exp Med. 2015;212(10):1571–1587. DOI: 10.1083/jcb.2106OIA178
Missero C. The genetic evolution of skin squamous cell carcinoma: tumor suppressor identity matters. Exp Dermatol. 2016. DOI: 10.1111/exd.13075. [Epub ahead of print]
Wood GS, Gunkel J, Stewart D, et al. Nonmelanoma skin cancers: basal cell and squamous cell carcinomas. In: Abeloff MD, Armitage JO, Niederhuber JE, Kastan MB, McKenna WG eds. Abeloff ’s Clinical Oncology. 4th ed. Philadelphia, PA: Churchill Livingston Elsevier; 2008:1253–1270.
Shulstad RM, Proper S. Squamous cell carcinoma: a review of etiology, pathogenesis, treatment and variants .J Dermatol Nurse Assoc. 2010;2(1):12–16. DOI: 10.1097/JDN.0b013e3181cecc51
Piipponen M, Nissinen L, Farshchian M, Riihilä P, Kivisaari A, Kallajoki M, et al. Long noncoding RNA PICSAR promotes growth of cutaneous squamous cell carcinoma by regulating ERK1/2 activity.J Invest Dermatol. 2016;136(8):1701–1710. DOI: 10.1016/j.jid.2016.03.028. Epub 2016 Apr 2.
Yang H, Schramek D, Adam RC, Keyes BE, Wang P, Zheng D, et al. ETS family transcriptional regulators drive chromatin dynamics and malignancy in squamous cell carcinomas. Elife. 2015;4:e10870. DOI: 10.7554/eLife.10870
Lotti R, Palazzo E, Petrachi T, Dallaglio K, Saltari A, Truzzi F, et al. Survivin modulates squamous cell carcinoma-derived stem-like cell proliferation, viability and tumor formation in vivo. Int J Mol Sci. 2016;17(1). pii: E89. DOI: 10.3390/ijms17010089
Shin JM, Chang IK, Lee YH, Yeo MK, Kim JM, Sohn KC, et al. Potential role of S100A8 in cutaneous squamous cell carcinoma differentiation. Ann Dermatol. 2016;28(2):179–185. DOI: 10.5021/ad.2016.28.2.179
Cancer Genome Atlas Network. Genomic classification of cutaneous melanoma. Cell. 2015;161(7):1681–1696. DOI: 10.1016/j.cell.2015.05.044
Ancuceanu R, Neagu M. Immune based therapy for melanoma. Indian Journal of Medical Research. 2016; 143(2):135–144. DOI: 10.4103/0971-5916.180197
Huang PH, Marais R. Melanoma troops massed. Nature. 2009;459(21):336–337. DOI: 10.1038/459336a
Russo AE, Torrisi E, Bevelacqua Y, Perrotta R, Libra M, McCubrey JA et al. Melanoma: molecular pathogenesis and emerging target therapies. Int J Oncol. 2009;34(6):1481–1489. DOI: 10.3892/ijo_00000277
Hocker TL, Singh MK, Tsao H. Melanoma genetics and therapeutic approaches in the 21st century: moving from the benchside to the bedside. J Invest Dermatol. 2008;128(11): 2575–2595. DOI: 10.1038/jid.2008.226
Chummun S, McLean NR. The management of malignant skin cancers. Surgery. 2014; 32(9):484–490. DOI: 10.1016/j.mpsur.2014.06.008
Neville Julie A, Welch E, Leffell David J. Management of nonmelanoma skin cancer in 2007. Nat Clin Pract Oncol. 2007;4:462e9. DOI: 10.1038/ncponc0883
Ericson MB, Wennberg A-M, Larkö O. Review of photodynamic therapy in actinic keratosis and basal cell carcinoma. Ther Clin Risk Manag. 2008;4(1):1–9. PMCID: PMC2503644
Kasper M, Jaks V, Hohl D, Toftgård R. Basal cell carcinoma—molecular biology and potential new therapies. J Clin Invest. 2012;122(2):455–463. DOI: 10.1172/JCI58779
Fantini F, Greco A, Del Giovane C, Cesinaro AM, Venturini M, Zane C, et al. Photodynamic therapy for basal cell carcinoma: clinical and pathological determinants of response. J Eur Acad Dermatol Venereol. 2011;25:896–901. DOI: 10.1111/j.1468-3083.2010.03877.x
Kessels J, Hendriks J, Nelemans P, Mosterd K, Kelleners-Smeets N. Two-fold illumination in topical 5-aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) for superficial basal cell carcinoma (sBCC): a retrospective case series and cohort study. J Am Acad Dermatol. 2016;74(5):899–906. DOI: 10.1016/j.jaad.2015.12.009
Roozeboom MH, Arits AH, Mosterd K, Sommer A, Essers BA, de Rooij MJ, et al.Three-year follow-up results of photodynamic therapy vs. imiquimod vs. fluorouracil for treatment of superficial basal cell carcinoma: a single-blind, non inferiority, randomized controlled trial. J Invest Dermatol. 2016;136(8):1568–1574. DOI: 10.1016/j.jid.2016.03.043
Sidoroff A, Thaler P. Taking treatment decisions in non-melanoma skin cancer—the place for topical photodynamic therapy (PDT). Photodiagnosis Photodyn Ther. 2010;7(1):24–32. DOI: 10.1016/j.pdpdt.2009.12.004
Ferrándiz C, Fonseca-Capdevila E, García-Diez A, Guillén-Barona C, Belinchón-Romero I, Redondo-Bellón P, et al. Spanish adaptation of the European guidelines for the evaluation and treatment of actinic keratosis. Actas Dermosifiliogr. 2014;105:378–393. DOI: 10.1016/j.adengl.2013.11.004
Zelickson B, Counters J, Coles C, Selim M. Light patch: preliminary report of a novel form of blue light delivery for the treatment of actinic keratosis. Dermatol Surg. 2005;31(3):375–378
Lucena SR, Salazar N, Gracia-Cazaña T, Zamarrón A, González S, Juarranz Á, et al. Combined treatments with photodynamic therapy for non-melanoma skin cancer. Int J Mol Sci. 2015;16(10):25912–25933. DOI: 10.3390/ijms161025912
Espinosa P, Pfeiffer RM, García-Casado Z, Requena C, Landi MT, Kumar R, et al. Risk factors for keratinocyte skin cancer in patients diagnosed with melanoma, a large retrospective study. Eur J Cancer. 2016;53:115–124. DOI: 10.1016/j.ejca.2015.10.058
Choromańska A, Kulbacka J, Chwiłkowska A, Skołucka N, Gamian A and Saczko J. Can photodynamic therapy be an alternative method in melanoma treatment? In: Ms. Morton R, editor. Treatment of Metastatic Melanoma. InTech; 2011. p. 271–294. DOI: 10.5772/20168
Sheleg SV, Zhavrid EA, Khodina TV, Kochubeev GA, Istomin YP, Chalov VN, et al. Photodynamic therapy with chlorin e(6) for skin metastases of melanoma. Photodermatol Photoimmunol Photomed. 2004;20(1):21–26.
Donaldson MJ, Lim L, Harper CA, Mackenzie J, Campbell GW. Primary treatment of choroidal amelanotic melanoma with photodynamic therapy. Clin Experiment Ophthalmol. 2005;33(5):548–549.DOI: 10.1111/j.1442-9071.2005.01083.x
Canal-Fontcuberta I, Salomão DR, Robertson D, Cantrill HL, Koozekanani D, Rath PP, et al. Clinical and histopathologic findings after photodynamic therapy of choroidal melanoma. Retina. 2012;32(5):942–948. DOI: 10.1097/IAE.0b013e31825097c1
Huang YY, Vecchio D, Avci P, Yin R, Garcia-Diaz M, Hamblin MR. Melanoma resistance to photodynamic therapy: new insights. Biol Chem. 2013;394(2):239–250. DOI: 10.1515/hsz-2012-0228
Camerin M, Moreno M, Marín MJ, Schofield CL, Chambrier I, Cook MJ, et al. Delivery of a hydrophobic phthalocyanine photosensitizer using PEGylated gold nanoparticle conjugates for the in vivo photodynamic therapy of amelanotic melanoma. Photochem Photobiol Sci. 2016;15(5):618–625. DOI: 10.1039/c5pp00463b
Menichini G, Alfano C, Marrelli M, Toniolo C, Provenzano E, Statti GA, et al. Hypericum perforatumL. subsp. perforatum induces inhibition of free radicals and enhanced phototoxicity in human melanoma cells under ultraviolet light. Cell Prolif. 2013; 46:193–202. DOI: 10.1111/cpr.12020.
Abrahamse H, Hamblin MR. New photosensitizers for photodynamic therapy. Biochem J. 2016;473(4):347–364. DOI: 10.1042/BJ20150942.
Dolmans DE, Fukumura D, Jain RK. Photodynamic therapy for cancer. Nat Rev Cancer. 2003;3(5):380–387. DOI: 10.1038/nrc1071
Piette J. Signalling pathway activation by photodynamic therapy: NF-κB at the crossroad between oncology and immunology. Photochem Photobiol Sci. 2015; 14:1510–1517. DOI: 10.1039/c4pp00465e
Castano AP, Demidova TN, Hamblin MR. Mechanisms in photodynamic therapy: part two-cellular signaling, cell metabolism and modes of cell death. Photodiagnosis Photodyn Ther. 2005;2(1):1–23. DOI: 10.1016/S1572-1000(05)00030-X
Ge X, Liu J, Shi Z, Jing L, Yu N, Zhang X, et al. Inhibition of MAPK signaling pathways enhances cell death induced by 5-Aminolevulinic acid-photodynamic therapy in skin squamous carcinoma cells. Eur J Dermatol. 2016; 26(2):164–172. DOI: 10.1684/ejd.2015.2725
Wan MT, Lin JY. Current evidence and applications of photodynamic therapy in dermatology. Clin Cosmet Investig Dermatol. 2014;7:145–163. DOI: 10.2147/CCID.S35334
Stockert JC, Cañete M, Juarranz A, Villanueva A, Horobin RW, Borrell JI, et al. Porphycenes: facts and prospects in photodynamic therapy of cancer. Curr Med Chem. 2007;14(9):997–1026. DOI: 10.2174/092986707780362934
Davids LM, Kleemann B. The menace of melanoma: a photodynamic approach to adjunctive cancer therapy. In: Guy Huynh Thien Duc, editor. Melanoma-from early detection to treatment. InTech; 2013. p. 583–628. DOI: 10.5772/53676
Ormond AB, Freeman HS. Dye sensitizers for photodynamic therapy. Materials. 2013;6:817–840. DOI: 10.3390/ma6030817
Il Y, Jia Zhu L, Young Key S. Advance in photosensitizers and light delivery for photodynamic therapy. Clin Endosc. 2013;46(1):7–23. DOI: 10.5946/ce.2013.46.1.7
Allison RR, Sibata CH. Oncologic photodynamic therapy photosensitizers: a clinical review. Photodiagnosis Photodyn Ther. 2010;7:61–75. DOI: 10.1016/j.pdpdt.2010.02.001
Anand S, Ortel BJ, Pereira SP, Hasan T, Maytin EV. Biomodulatory approaches to photodynamic therapy for solid tumors. Cancer Lett. 2012; 326(1):8–16. DOI: 10.1016/j.canlet.2012.07.026
Almeida RD, Manadas BJ, Carvalho AP, Duarte CB. Intracellular signaling mechanisms in photodynamic therapy. Biochimicaet Biophysica Acta. 2004;1704(2):59–86. DOI: 10.1016/j.bbcan.2004.05.003
Gołab J, Olszewska D, Mróz P, Kozar K, Kamiński R, Jalili A, et al. Erythropoietin restores the antitumor effectiveness of photodynamic therapy in mice with chemotherapy-induced anemia. Clin Cancer Res. 2002;8:1265–1270.
Mroz P, Yaroslavsky A, Kharkwal GB, Hamblin MR. Cell death pathways in photodynamic therapy of cancer. Cancer. 2011;3:2516–2539. DOI: 10.3390/cancers3022516
O’Connor AE, Gallagher WM, Byrne AT. Porphyrin and nonporphyrin photosensitizers in oncology: preclinical and clinical advances in photodynamic therapy. Photochem Photobiol. 2009;85:1053–1074. DOI: 10.1111/j.1751-1097.2009.00585.x
Debele TA, Peng S, Tsai HC. Drug carrier for photodynamic cancer therapy. Int J Mol Sci. 2015;16(9):22094–22136. DOI: 10.3390/ijms160922094
Cadet J, Douki T, Ravanat JL. Oxidatively generated damage to the guanine moiety of DNA: mechanisticaspects and formation in cells. Acc Chem Res. 2008;41(8):1075–1083. DOI: 10.1021/ar700245e
Shan X, Chang Y, Lin CG. Messenger RNA oxidation is an early event preceding cell death and causes reduced protein expression. FASEB J. 2007;21:2753–2764. DOI: 10.1096/fj.07-8200com
Sakharov DV, Elstak EDR, Chernyak B,Wirtz KWA. Prolonged lipid oxidation after photodynamic treatment. Study with oxidation-sensitive probe C11-BODIPY581/591. FEBS Lett. 2005;579:1255–1260. DOI: 10.1016/j.febslet.2005.01.024
Szokalska A, Makowski M, Nowis D, Wilczynski GM, Kujawa M, Wójcik C, et al. Proteasome inhibition potentiates antitumor effects ofphotodynamic therapy in mice through induction of endoplasmic reticulum stress and unfolded protein response. Cancer Res. 2009;69:4235–4243. DOI: 10.1158/0008-5472.CAN-08-3439
Catalá A. Lipid peroxidation of membrane phospholipids generates hydroxy-alkenals and oxidized phospholipids active in physiological and/or pathological conditions. Chem Phys Lipids. 2009;157:1–11. DOI: 10.1016/j.chemphyslip.2008.09.004
Yukawa O, Nagatsuka S, Nakazawa T. Reconstitution studies on the involvement of radiation-induced lipid peroxidation in damage to membrane enzymes. Int J Radiat Biol Relat Stud Phys Chem Med. 1983;43(4):391–398. DOI: 10.1080/09553008314550451
Hilf R. Mitochondria are targets of photodynamic therapy. J Bioenerg Biomembr. 2007;39(1):85–89. DOI: 10.1007/s10863-006-9064-8
Wachowska M, Muchowicz A, Demkow U.Immunological aspects of antitumor photodynamic therapy outcome. Cent Eur J Immunol. 2015;40(4): 481–485. DOI: 10.5114/ceji.2015.56974
Coupienne I, Fettweis G, Rubio N, Agostinis P, Piette J. 5-ALA-PDT induces RIP3-dependent necrosis in glioblastoma. Photochem Photobiol Sci. 2011;10(12):1868–1878. DOI: 10.1039/c1pp05213f
Reiners JJ, Agostinis P, Berg K, Oleinick NL,Kessel DH. Assessing autophagy in the context of photodynamic therapy. Autophagy. 2010;6(1):7–18.
Garg AD, Krysko DV, Vandenabeele P, Agostinis P. DAMPs and PDT-mediated photo-oxidative stress: exploring the unknown. Photochem Photobiol Sci. 2011;10(5):670–680. DOI: 10.1039/c0pp00294a.
Mroz P, Hashmi JT, Huang YY, Lange N, Hamblin MR.Stimulation of anti-tumor immunity by photodynamic therapy. Expert Rev Clin Immunol. 2011;7(1):75–91. DOI: 10.1586/eci.10.81.
Broekgaarden M, Weijer R, van Gulik TM, Hamblin MR, Heger M. Tumor cell survival pathways activated by photodynamictherapy: a molecular basis for pharmacologicalinhibition strategies. Cancer Metastasis Rev 2015;34:643–690. DOI: 10.1007/s10555-015-9588-7
Gęgotek A, Skrzydlewska E. The role of transcription factor Nrf2 in skin cells metabolism. Arch Dermatol Res. 2015;307(5):385–396. DOI: 10.1007/s00403-015-1554-2
Hoesel B, Schmid JA. The complexity of NF-κB signaling in inflammation and cancer. Mol Cancer. 2013;12:86. DOI: 10.1186/1476-4598-12-86
Kim C, Pasparakis M. Epidermal p65/NF-κB signalling is essential for skin carcinogenesis. EMBO Mol Med. 2014; 6(7):970–983. DOI: 10.15252/emmm.201303541
Yang J, Splittgerber R, Yull FE, Kantrow S, Ayers GD, Karin M, et al. Conditional ablation of Ikkb inhibits melanoma tumor development in mice. J Clin Invest. 2010;120:2563–2574. DOI: 10.1172/JCI42358.
Singh M, Suman S, Shukla Y. New enlightenment of skin cancer chemoprevention through phytochemicals: in vitro and in vivo studies and the underlying mechanisms. Biomed Res Int. 2014;2014:243452. DOI: 10.1155/2014/243452
Mitra S, Cassar SE, Niles DJ, Puskas JA, Frelinger JG, et al. Photodynamic therapy mediates the oxygen-independent activation of hypoxia-inducible factor 1α. Mol Cancer Ther. 2006;5:3268–3274. DOI: 10.1158/1535-7163.MCT-06-0421
Tao JS, Sanghera JS, Pelech SL, Wong G, Levy JG. Stimulation of stress-activated protein kinase and p38HOG1 kinase in murine keratinocytes following photodynamic therapy with benzoporphyrin derivative. J Biol Chem. 1996;271(43):27107–27115.
Hetz C. The unfolded protein response: controlling cell fate decisions under ER stress and beyond. Nat Rev Mol Cell Biol. 2012; 13(2):89–102. DOI: 10.1038/nrm3270
Koumenis C. ER stress, hypoxia tolerance and tumor progression. Curr Mol Med. 2006;6:55–69.
Dougherty TJ, Grindey GB, Fiel R, Weishaupt KR, Boyle DG. Photoradiation therapy. II. Cure of animal tumors with hematoporphyrin and light. J Natl Cancer Inst. 1975;55(1):115–121.
Evans CL. Three-dimensional in vitro cancer spheroid models for photodynamic therapy: strengths and opportunities. Front Phys. 2015;3:15. DOI: 10.3389/fphy.2015.00015
Chinembiri TN, du Plessis LH, Gerber M, Hamman JH, du Plessis J. Review of natural compounds for potential skin cancer treatment. Molecules. 2014;19:11679–11721. DOI: 10.3390/molecules190811679
Popovic A, Wiggins T, Davids LM. Differential susceptibility of primary cultured human skin cells to hypericin PDT in an in vitro model. J Photochem Photobiol B. 2015;1(49):249–256. DOI: 10.1016/j.jphotobiol.2015.06.009
Park SI, Hwang YH, Lim JH, Kim JH, Yun HI, Kim CO. Biological and thermic effects of magnetic fluids for photodynamic therapy and hyperthermia. J Magn Magn Mater. 2006; 304:e403–e405. DOI: 10.1016/j.jmmm.2006.01.204
Bolfarini GC, Siqueira-Moura MP, Demets GJF, Morais PC, Tedesco AC. In vitroevaluation of combined hyperthermia and photodynamic effects using magnetoliposomes loaded with cucurbit uril zinc phthalocyanine complex on melanoma. J Photochem Photobiol B. 2012;115:1–4. DOI: 10.1016/j.jphotobiol.2012.05.009
McEwan C, Nesbitt H, Nicholas D, Kavanagh ON, McKenna K, Loan P, et al.. Comparing the efficacy of photodynamic and sonodynamic therapy in non-melanoma and melanoma skin cancer. Bioorg Med Chem. 2016;24(13):3023–3028. DOI: 10.1016/j.bmc.2016.05.015
Idris NM, Gnanasammandhan MK, Zhang J, Ho PC, Mahendran R, Zhang Y. In vivo photodynamic therapy using up conversion nanoparticles as remote-controlled nanotransducers. Nat Med. 2012;18(10):1580–1585. DOI: 10.1038/nm.2933
Chen J, Shao R, Zhang XD, Chen C. Applications of nanotechnology for melanoma treatment, diagnosis, and theranostics. Int J Nanomedicine. 2013;8:2677–2688. DOI: 10.2147/IJN.S45429
Cordwell SJ, Thingholm TE. Technologies for plasma membrane proteomics. Proteomics. 2010;10:611–627. DOI: 10.1002/pmic.200900521
Franssen ME, Zeeuwen PL, Vierwinden G, Van De Kerkhof PC, Schalkwijk J, Van Erp PE. Phenotypical and functional differences in germinative subpopulations derived from normal and psoriatic epidermis. J Invest Dermatol. 2005;124(2):373–383. DOI: 10.1111/j.0022-202X.2004.23612.x
Davies H, Bignell GR, Cox C, Stephens P, Edkins S, Clegg S, et al. Mutations of the BRAF gene in human cancer. Nature. 2002;417(6892):949–954. DOI: 10.1038/nature00766
Medina T, Amaria MN, Jimeno A. Dabrafenib in the treatment of advanced melanoma. Drugs Today (Barc). 2013;49(6):377–385. DOI: 10.1358/dot.2013.49.6.1968669
Mesri M. Advances in proteomic technologies and its contribution to the field of cancer. Adv Med. 2014;2014:238045. DOI: 10.1155/2014/238045
Boja E, Hiltke T, Rivers R, Kinsinger C, Rahbar A, Mesri M, et al. Evolution of clinical proteomics and its role in medicine. J Proteome Res. 2011;10(1):66–84. DOI: 10.1021/pr100532g
Roy P, Shukla Y. Applications of proteomic techniques in cancer research. Cancer Ther. 2008;6:841–856.
von Felbert V, Bauerschlag D, Maass N, Bräutigam K, Meinhold-Heerlein I, Woitok M, et al. A specific photoimmunotheranostics agent to detect and eliminate skin cancer cells expressing EGFR. J Cancer Res Clin Oncol. 2016;142(5):1003–1011. DOI: 10.1007/s00432-016-2122-7
Huang SK, Darfler MM, Nicholl MB, You J, Bemis KG, Tegeler TJ, et al. LC/MS-based quantitative proteomic analysis of paraffin-embedded archival melanomas reveals potential proteomic biomarkers associated with metastasis. PLoS One. 2009;4(2):e4430. DOI: 10.1371/journal.pone.0004430
Griffin NM, Yu J, Long F, Oh P, Shore S, Li Y, et al. Label-free, normalized quantification of complex mass spectrometry data for proteomic analysis. Nat Biotechnol. 2010;28(1):83–89. DOI: 10.1038/nbt.1592
Wilson LL, Tran L, Morton DL, Hoon DS. Detection of differentially expressed proteins in early-stage melanoma patients using SELDI-TOF mass spectrometry. Ann N Y Acad Sci. 2004;1022:317–322. DOI: 10.1196/annals.1318.047
Zhou M, Veenstra TD. Mass spectrometry: m/z 1983–2008. Biotechniques. 2008;44(5):667–668, 670. DOI: 10.2144/000112791
Helander L, Sharma A, Krokan HE, Plaetzer K, Krammer B, Tortik N, et al. Photodynamic treatment with hexyl-aminolevulinate mediates reversible thiol oxidation in core oxidative stress signaling proteins. Mol Biosyst. 2016;12(3):796–805. DOI: 10.1039/c5mb00744e
Tu S, Jiang HW, Liu CX, Zhou SM, Tao SC. Protein microarrays for studies of drug mechanisms and biomarker discovery in the era of systems biology. Curr Pharm Des. 2014;20(1):49–55.
Matei C, Tampa M, Caruntu C, Ion RM, Georgescu SR, Dumitrascu GR, et al. Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy. Biol Res. 2014;47:33. DOI: 10.1186/0717-6287-47-33