Effect of substrates on plant height and number of leaves of tomato during vegetation
The most innovative technology of plants growing in greenhouses is growing plants in mineral substrates such as rockwool, vermiculite, perlite, zeolite, ceramsite and others. The origin of substrates is different, some of them are of natural origin while others are produced artificially [1-3]. They also differ in their physical, chemical, and biological properties. Therefore, substrate selection is one of the most important factors affecting plant growth and development in the greenhouse and influencing vegetable quality.
Vegetable-growing rockwool is a widely used substrate for growing of tomatoes and cucumbers under commercial production system. However, one of the biggest disadvantages of this substrate is the need to utilize it. Currently for growing vegetables’ different natural substrates are also used and one of these is coconut fiber [4-7]. Substrates of coconut fiber are produced in most countries (like Polland, Netherlands, Belgium, Chezch Republic). Current recycling technologies allow to produce different products in its quality which have its advantages compared to other substrates which are used in greenhouses for growing vegetable . Ready to use coconut fiber substrate may look like dry brick, non-pressed pack as well as blocks’ shape. Blocks of coconut fiber are widely used in floriculture, especially for growing roses and gerbera [9,10]. Coconut fiber is a absolutely (100%) organic substrate which is made from recycling the shells of coconuts. It is inert substrate as it does not dissolve upon utilization, size does not change but restrains huge amount of water (more than rockwool). Coconut fiber has other properties such as it is typical to absorb warmth, do not get salty, and it has no pathogens and seeds of weeds [6,11,12]. Substrate of coconut fiber is an alternative for rockwool, no problems appears after utilization. This substrate also has its advantages over rockwool for example; structure of coconut fiber does not change for several years due to its high lignin content. The same structure lasts for 3-4 years. In this way, substrate may be used for few years . Results of last investigations showed that coconut fiber were sufficient substrates for growing of some plants especially for vegetables and grower use these materials as growing media in greenhouse cultures . Albaho and others  argued that coconut fiber and its mixes with other substrates could be used as alternative substrate for tomato growing.
Peat and their mixes with perlite, vermiculite, zeolite are the most widely used substrates in greenhouse. In most countries there are analyzed features of zeolite and possibilities to use it for growing of vegetables [15-17]. Zeolites are hydrated crystalline aluminosilicate minerals of natural occurence, structured in rigid third dimension net. This is ecologically clean, inert and non-toxic substance. It is characterized by ion exchange and adsorption features [18,19]. According to Russian scientist, one of the most promising fields of plant-growing is use of natural zeolite as a substrate for seedlings and vegetables to grow [20,21]. There are different reports related to use of zeolite as substrates in hydroponic culture. Technologies of growing cucumbers, tomatoes and green vegetables in zeolite were created in Russia [17,22]. There were also analyzed opportunities how zeolite as a substrate and its mixes with peat could be used in greenhouses [16,19]. It was found that using zeolite less nutrients is missed, efficiency of mineral fertilizer increases. There were analyzed options of using zeolite to grow seddlings of vegetables as well as potted plants [23-25]. Gül and others  concluded that the use of zeolite led to increased lettuce plant growth. Most scientific researches reveal the effect of substrates for vegetables productivity [27-29]. Gruda  states that it is possible to improve the quality of fruit if suitable substrate is chosen. Other researches show effect of substrates and its mixes for vegetable quality [23,29,31-33].
The aim of this study was to estimate of rockwool and coconut fiber substrates on productivity and quality of tomato hybrids ‘Raissa’ and ‘Admiro’. In addition to establish the optimal amount of zeolite in peat substrate and to evaluate the influence of zeolite-peat mixes on productivity and quality of tomato hybrid ‘Ronaldo’.
2. Materials and methods
2.1. Growing conditions
The investigations were carried out at the Institute of Horticulture, in the Multi Rovero 640 TR (“Rovero”, the Netherlands) greenhouse covered with a double polymer film. The tomatoes were sown at the beginning of February and the seedlings were grown in rockwool growing cubes on the shelvings in a heated nursery and lighted additionally by high pressure sodium lamps (Philips SON-T Agro). At the beginning of March the seedlings were transplanted in the greenhouse (Figure 1). The plant density in the greenhouse was 2.5 plants per m-2. The end of tomato vegetation was the middle of October. Two factors were investigated: factor A – tomato hybrids: a0 – ‘Raissa’, a1 – ‘Admiro’, factor B – substrate: b0 – rockwool, b1 – coconut fiber. Plot area – 8 m2. Four replications were done in a randomized block design.
The investigations were carried out at the Institute of Horticulture, in the Multispan 9.60 SR (“Richel”, France) greenhouse covered with a double polymer film. The tomato seedlings were grown in polymer pots filled with peat substrate (Profi 1, Durpeta, Lithuania) (pH 5-6) on the shelvings in a heated nursery and lighted additionally by high pressure sodium lamps (Philips SON-T Agro). In the greenhouse the plants were grown in 25 l peat bags (1 bag – 2 plants) (Figure 2). The plant density was 2.5 plants per m-2. The start of tomato vegetation was the beginning of February and the end was the middle of October. The investigation object was hybrid ‘Ronaldo’. Different substrates were investigated: a0 – peat, a1 – peat + zeolite (15%), a2 – peat + zeolite (30%). Plot area – 9.6 m2. Three replications were done in a randomized block design.
2.2. Cultivation procedure
In both greenhouses the tomatoes were grown using drip irrigation and fertilized with “Nutrifol” (green, NPK 8-11-35 plus microelements - S, MgO, Mn, B, Zn, Cu, Co, Mo, Fe) (first half of the vegetation), “Nutrifol” (brown, NPK 14-10-25 plus microelements - S, MgO, Mn, B, Zn, Cu, Co, Mo, Fe) (second half of vegetation), magnesium sulphate, calcium and ammonium nitrate fertilizers. There was prepared solution, which was diluted with water in a ratio of 1: 100, and plants were fertilized taking into the account the growth stage (4-15 times a day). Nitric acid was used for water acidification. The concentration of salts in the nutrient solution was EC 2.6–3.0, acidity – pH 5.5–5.8.
2.3. Biometric measurements
During the investigation the plant height was measured at three times during vegetative growth each 10 days after transplanting the seedlings in the greenhouse and the leaves were also counted.
2.4. Determination of photosynthetic pigments and dry matter
For sample preparation of photosynthetic pigment 0.2 g of fresh weight were ground with 0.5 g CaCO3 (Sigma-Aldrich, Germany) and extracted in 100% acetone (Merck, Germany), according to Vetsthtein . Spectrophotometric analysis (spectrophotometer Genesys 6, USA) and quantification of total chlorophylls
To determine dry weight tomato leaves and fruits were dried in a drying oven (Venticell,MBT, Czech Republik) at 105 °C for 24 h. The content of dry matter and photosynthetic pigments in leaves were established at three times during entire growth phase, such as measurement I - at the beginning of flowering, measurement II – at the start of yielding, measurement III – at full yielding.
2.5. Phytomonitoring investigations
The phytomonitoring investigations were carried out on the tomatoes grown in different substrates. The physiological processes of tomato ‘Raissa‘ F1 were investigated using a phytometric system “LPS-03” created by “PthyTech Ltd.”(Figure 3). The following sensors were used for the investigations such as sap water flow, stem diameter evolution, fruit diameter evolution and leaf-air temperature differences (Figure 4). The data of these sensors reflect the plant response to various growing conditions best. In addition, microclimate parameter sensors (those of air temperature and total irradiance) were used. The sensors were fixed according to “PhyTech Ltd.” recommendations [35,36]. The sensors of stem diameter evolution, stem flux rate and leaf-air temperature were used as indirect indicators of transpiration. The plants were measured for five days.
2.6. Yielding of plants
The tomato yield was recorded at every harvest. Tomato fruits were harvested three times a week, next they were separated into marketable and non-marketable ones. Total yield were calculated by aggregating each harvest.
2.7. Biochemical analysis
The biochemical composition of tomato fruits was investigated at the Laboratory of Biochemistry and Technology, Institute of Horticulture. The following methods were applied in establishing the composition: sugars – by AOAC method , carotenoids – spectrophotometrically by Genesys10 UV/VIS spectrophotometer (Thermo Spectronic, Rochester, USA) , nitrates – by potentiometrical method using an ion selective electrode . The total soluble solids were determined by a digital refractometer (ATAGO PR-32, Atago Company, Japan). The dry matter content was determined by the air oven method after drying at 105 °C in a Universal Oven ULE 500 (Memmert GmbH+Co. KG, Schwabach, Germany) to a constant weight . Ascorbic acid content was measured by titration with 2.6-dichlorphenolindophenol sodium salt using chloroform for intensely coloured extracts . Titrable acidity was measured by titrating 10 g of pulp that had been homogenised with 100 ml distilled water. The initial pH of the sample was recorded before titration with 0.1 N NaOH to final pH 8.2. The acidity was expressed as the percentage of citric acid equivalent to the quantity of NaOH used for the titration.
2.8. Statistical analysis
The data were analysed by ANOVA statistical package . The Fisher’s LSD was used to determine significant treatment effects. Statistical significance was evaluated at p≤0.05.
3.1. Effect of rockwool and coconut fiber substrates on productivity, physiological processes and quality of tomato
During vegetation the tomato hybrids grown in different substrates grew and developed differently. The height and leaf number depended both on the substrate used and on the hybrid itself (Table 1). Tomatoes ‘Raissa‘ F1 grown in a coconut fiber substrate were 8.1–9.2% higher (insignificant difference) compared with the plants grown in rockwool. Moreover, they had a larger number of leaves. The plants of hybrid ‘Admiro’ grown in the coconut fiber substrate were slightly lower during the first and the second measures taken (insignificant difference) compared with those grown in rockwool. During the third measure taking the height of this hybrid was equal in both substrates. The ‘Admiro‘ plants grown in rockwool and coconut fiber had the same number of leaves.
The content of dry matter in the tomato leaves depended on the substrate (Figure 5). Both hybrids grown in rockwool accumulated a higher content of dry matter in their leaves during vegetation compared with those grown in coconut fiber. The content of dry matter in the leaves of tomatoes ‘Raissa’ F1 grown in rockwool was 2.6–8.1% higher (depending on measuring) compared with those grown in the coconut fiber substrate (Figure 5 a). The content of dry matter in the leaves of tomato hybrid ‘Admiro’ grown in rockwool was higher in all measures taken (Figure 5 b) (insignificant differences).
The photosynthetic pigment content in the leaves of tomatoes depended on the substrate as well (Table 2). A higher amount of photosynthetic pigments was accumulated in the leaves of both hybrids grown in rockwool. The chlorophyll a + b amount in the leaves of tomato hybrid ‘Raissa’ was higher by 3.4%; in the case of tomato hybrid ‘Admiro’ it was 7.0% higher compared with the leaves of the tomatoes grown in the coconut fiber substrate. The chlorophyll a to b ratio in the leaves of both tomato hybrids grown in different substrates was almost similar. The content of carotenoids in the leaves of the tomatoes grown both in rockwool and coconut fiber was more or less the same during vegetation. A slightly lower content was accumulated in the tomatoes grown in the coconut fiber substrate.
The intensity of photosynthesis depended on the hybrid of tomato (Figure 6). The photosynthesis intensity of tomato hybrid ‘Admiro’ was slightly higher compared with those grown in the coconut fiber substrate. The highest intensity of photosynthesis was established with tomato hybrid ‘Raissa’, when grown in the coconut fiber substrate.
The phytomonitoring investigations were carried out for five days. During the investigations the air temperature within the plant growing zone was about 25 °C and judging from the total irradiance fluctuations the days were overcast with gaps in the clouds (Figure 7). According to the stem flux rate, stem diameter evolution and the difference in leaf-air temperature it is possible to assess the intensity of transpiration and the overall turnover of water in a plant (Figures 8 and 9). The variation of these indicators during 24 hours was similar both in the tomatoes grown in rockwool and in the coconut fiber substrate. In the middle of the day the stem flux rate increased, the stem diameter decreased and the leaf temperature was practically always lower than that of the air. Therefore, it can be proposed that the transpiration in tomatoes was very intensive and a low stem gain per 24 hours indicates that the plants were not supplied with water sufficiently. The tomatoes grown in coconut fiber demonstrated a higher leaf-air temperature difference compared with the tomatoes grown in rockwool. It can be proposed that the transpiration of the latter was less intensive. The more intensive transpiration in the tomatoes grown in coconut fiber had negative influence on fruit growth. Typically, fruits have to grow in a uniform fashion and this substrate practically stopped the daily growth and the growth returned to normal only in the second half of the night (Figure 10). The size increase of the tomatoes grown in rockwool was more uniform. Their growth slowed down in the middle of the day but it returned to normal again in the evening. It can be proposed that the tomatoes grown in coconut fiber substrate demonstrated a higher water demand compared with the tomatoes grown in rockwool.
The coconut fiber substrate had positive effect on the tomato yield (Figure 11). The yield of tomato hybrids ‘Raissa’ and ‘Admiro’ grown in coconut fiber was higher compared with those grown in rockwool (insignificant differences). The yield of tomato hybrid ‘Admiro’ was significantly higher compared with the yield of tomato hybrid ‘Raissa’ as there were more trusses on the plants formed and the number of fruits in a truss was higher. Somewhat higher early yield was obtained from the tomatoes grown in rockwool. The yield of non-marketable fruits from the tomatoes grown in different substrates was the same: it was 0.24 kg m-2 from tomato hybrid ‘Raissa' in both substrates and 0.4 kg m-2 from tomato hybrid ‘Admiro‘.
Tomato hybrid ‘Raissa’ formed 15 trusses both in rockwool and coconut fiber, however the number of fruits in a truss was different: the number was slightly higher in rockwool compared with coconut fiber (Table 3). Tomato hybrid ‘Admiro’ formed 15.5 trusses both in rockwool and coconut fiber and the number of fruits was the same. The substrate had no great influence on the average mass of a fruit. The fruit mass of the tomatoes grown in rockwool was slightly higher compared with those grown in coconut fiber. The fruits of tomato hybrid ‘Raissa’ were somewhat larger – the average mass of a fruit ranged from 137.1 g to 140.1 g, and the average mass of ‘Admiro’ fruit was between 131.0 g and 135.4 g.
Growing of tomatoes in different substrates had influence on the biochemical composition of fruits (Table 4). Tomato hybrid ‘Raissa’ grown in rockwool accumulated a higher amount of sugars, dry soluble solids and dry matter (insignificant difference). The amount of ascorbic acid in the fruits of the tomatoes grown in coconut fiber was 1.1 times higher compared with the fruits of the tomatoes grown in rockwool (insignificant difference). Different substrates had influence on the amount of nitrates in tomato fruits: the amount was higher in the tomato fruits grown in rockwool (insignificant difference).
3.2. Effect of peat and peat-zeolite substrates on productivity and quality of tomato
The admixture of zeolite into a peat substrate had effect on the height of plants. The tomatoes grown in peat-zeolite substrates were lower compared with those grown in peat (Table 5). The tomatoes grown in the peat + zeolite (30%) substrate were 3.1–5.9% lower (depending on measuring) compared with the plants grown in the peat substrate. The tomatoes grown in the peat + zeolite (15%) substrate were the lowest. A lower concentration of zeolite in peat had a greater effect on vegetative plant growth, i. e., the overground mass developed better. An asumption can be made that a greater concentration of zeolite had an effect of better root development but not on the overground plant section.
|Peat + zeolite (15%)||51.1||69.9||100.0|
|Peat + zeolite (30%)||48.1||67.0||96.9|
At the beginning of vegetation the amount of dry matter in the leaves of the tomatoes grown in peat was higher compared with those grown in peat and zeolite substrates (Table 6). It was 5.3–8.2% more (Measurement I and II, respectively) compared with the leaves of the tomatoes grown in the peat + zeolite (15%) substrate and 1.1–11.6 % more (Measurement I and II, respectively) compared with the leaves of the tomatoes grown in the peat + zeolite (30%) substrate (insignificant differences). During the Measurement III it was established that the highest amount of dry matter in leaves was accumulated by the tomatoes grown in the peat + zeolite (30%) substrate. The amount was 7.5% higher compared with the leaves of the tomatoes grown in peat and 11.1% higher compared with the leaves of the tomatoes grown in the peat + zeolite (15%) substrate (significant difference). During the entire vegetation the lowest amount of dry matter was accumulated in leaves of the tomatoes grown in the peat + zeolite (15%) substrate.
The content of dry matter in the fruits of the tomatoes grown in different substrates during vegetation was different. The lowest amount of dry matter in the fruits was demonstrated by the tomatoes grown in the peat + zeolite (15 %) substrate. The average data of three measures revealed that the highest amount of dry matter in fruits was accumulated by the tomatoes grown in the peat + zeolite (30 %) substrate and it amounted to 6.4%.
|Peat + zeolite (15%)||10.24||5.41||9.79||4.49||9.74||6.10|
|Peat + zeolite (30%)||9.93||5.97||10.20||6.77||10.82||6.47|
The tomatoes grown in peat and peat + zeolite (30%) substrates accumulated a higher content of chlorophyll compared with the tomatoes grown in the peat + zeolite (15%) substrate (Table 7). The content of chlorophyll was 10.5 %, chlorophyll b – 11.9% and chlorophyll a + b – 10.9% higher compared with the leaves of the tomatoes grown in the peat + zeolite (15%) substrate. The highest chlorophyll a to b ratio was established in the leaves of the tomatoes grown in peat. The carotenoid content was almost the same in the leaves of the tomatoes grown in all substrates.
|Peat + zeolite (15%)||1.05||0.42||1.47||2.50||0.30|
|Peat + zeolite (30%)||1.16||0.47||1.63||2.47||0.32|
Zeolite had effect on yield earliness (Figure 12). During the first month of fruiting it ranged from 7.0 kg m–2 to 8.0 kg m–2 (depending on the substrate). The highest early yield was obtained while growing tomatoes in the peat + zeolite (15%) substrate. It was 11.1% higher compared with the tomatoes grown in peat alone and 14.3% higher than that obtained from the tomatoes grown in the peat + zeolite (30%) substrate (insignificant differences). The total yield was higher in the plants grown in peat and zeolite substrates. The extra yield depended on the amount of zeolite in peat. The yield of the tomatoes grown in the peat + zeolite (15%) substrate was 10.3% (significant difference) higher than that of the tomatoes grown in the peat substrate alone. This effect was related with zeolite’s property to accumulate and retain and then release the nutrients to the plants in due time. However, the admixture of higher amounts of zeolite to the substrate had practically no effect on the tomato yield.
The tomatoes grown in different substrates formed the same number of fruits in a truss. In all treatments the number of fruits per truss was between 4.40 and 4.44 u. However, the average fruit mass was slightly different between the treatments and ranged from 133.2 g to 138.1 g (Figure 13). The largest were the fruits of the tomatoes grown in the peat + zeolite (15%) substrate: their mass was 1.9 % higher compared with the tomatoes grown in peat only and 3.7% higher than tomato fruits in the peat + zeolite (30%) substrate (insignificant differences).
The admixture of zeolite into the peat substrate had influence on the biochemical composition of the tomato fruits (Table 8). The fruits of the tomatoes grown in peat-zeolite substrates accumulated less sugars, ascorbic acid and soluble solids. The admixture of zeolite into the peat substrate resulted in a 17.8 -19.6% higher titratable acidity amount (significant difference) compared with the tomatoes grown in peat. In addition, they accumulated a slightly higher amount of carotenoids.
4.1. Effect of rockwool and coconut fiber substrates on productivity, physiological processes and quality of tomato
Results of most scientists researches showed that substrates had a significant effect on the plant growth, composition of leaf, total yield and fruit quality [27,31,42,43]. Researchers from different countries analysed the suitability of coco substrates (coconut dust, coco fiber and its mixtures) for growing of vegetables in greenhouses. Data of Lopez et al.  showed substrate of coconut dust is proper for growing of tomatoes in greenhouses. It is also characterised as substrate with higher qualities than Canadian peat. Researches of other scientists proved substrates of coconut fiber may be used as organic substrate for growing of plants . In other researches coco substrates was compared to other substrates used in greenhouse vegetable-growing. There was analysed yield of plants while growing vegetables in coco substrate, perlite, rockwool, sawdust, rise husks [12,44-47]]. Coconut fiber was compared to substrate which is produced from waste composting 48]. Alifar et al.  investigated the effect of five different growing media for cucumbers’ growing. Results showed that the largest stem diameter, the highest biomass were obtained in cocopeat and perlite-cocopeat media. Fecondini and others  data reveal hybrid more than substrate where plants were grown in had more influence for phenological observations and biometric parameters of tomatoes. Our data showed the tomatoes grown in rockwool and coconut fiber grow evenly, height of the plants did not differ a lot.
In our research, physiological processes in tomatoes grown in coconut fiber substrate were similar to that were grown in rockwool. Increasing sap water flow, decreased diameter of stem and bigger difference of leaves and air temperatures showed that more intense transpiration was in tomatoes grown in coconut fiber substrate than in plants grown in rockwool (Figure 8 and 9). It may be assumed this had an influence for non-uniform fruit growth in this substrate compared to rockwool (Figure 10). According to physiological researches’ data it may be stated that absorption of substrates of coconut fiber and rockwool for water is different.
The content of carotenoid and chlorophyll pigments in the vegetables depend on growing conditions as well as on variety of vegetable [51,52]. According to Islam at al.  data there was no difference between content of chlorophyll and dry matter in the leafs of tomatoes grown in coconut fiber and rockwool substrates. There also was no difference on the amount of ascorbic acid in the fruits of tomatoes in these substrates. Our data showed the tomatoes grown in rockwool accumulated more dry matters and pigments of photosynthesis than those grown in coconut fiber substrate.
Various data show substrate has an influence on the yield of tomatoes. Some researches showed yield of tomatoes grown in coconut fiber substrates was higher than grown in other substrates, another researches did not show any difference in the yield [12,48,54]. Kobryn  stated bigger yield was got growing tomatoes in rockwool than in substrate Cocovita which is made of coconut palms straw. Jensen’s  research show there are no fundamental differences between the yield of tomatoes grown in different substrates (perlite, rockwool, coconut, etc.). Carrijo et al.  researches state tomatoes grown in coconut substrate were more fertile than those grown in sawdust. Halman  data show yield of cherry tomatoes grown in coconut and rockwool was similar. The tomatoes were grown in rockwool, peat, coconut fiber with a different admixture of chips. According to research data, the significantly highest total yield of plants was found in the case of plants grown in peat and in coconut fiber with a higher content of chips in relation to rockwool . Our data showed the yield of tomatoes grown in coconut fiber was little bit higher than those grown in rockwool.
Most scientists found the type of substrate affected the quality of tomato fruit [28,48,58]. Selection of substrate has an influence not only on the yield of plants but on quality of the fruits as well as its beginning of yielding . Growth, yield and fruit quality of tomato grown in coconut fiber were not different from those grown in rockwool . Hallman  states the tomatoes grown in cocos substrate had more sugar, acids, there also was less ascorbic acid and licopene. Our data showed growing of tomatoes in rockwool and coconut substrate had an influence on the biochemical composition of fruits (Table 4). Tomato hybrid ‘Raissa‘ grown in rockwool accumulated more sugar, dry soluble solids and dry matter in the fruits but tomatoes grown in coconut fiber accumulated more ascorbic acid. The substrate had no influence on the average mass of a fruit. Our data showed the average mass of fruits was pretty similar growing tomatoes both in coconut fiber and rockwool.
4.2. Effect of peat and peat-zeolite substrates on productivity and quality of tomato
Zeolite and coco substrates may be used in two ways: first, it may be used as a part of mixture of substrates, second, it may be used as only substrate for vegetable growing. There were researches done analyzing growth of tomatoes in mixed perlite and zeolite substrates, mixing both in different ratios [60,61]. According to data, better results were achieved growing tomatoes in zeolite and perlite substrates (ratio 1:1) than growing in cocos and perlite substrates . There also was analyzed the influence of zeolite mixed with other substrates for peppers’, lettuce and various flowers yield as well as productivity [15,26,62]. Russian scientists results show lettuce grown in peat and zeolite substrates had smaller content of nitrates, there was bigger yield of cucumbers. Growing vegetable in zeolite substrate there was lower use of fertilizers [20,63]. Our data showed the admixture of zeolite into a peat substrate did not have significant influence for growth of plants. The tomatoes grown in peat and zeolite substrates were a little bit lower than those plants grown in peat.
Seeking for better evaluation of the influence of mixtures of substrates on the plants growth, there was found content of photosynthetic pigments in the leafs of tomatoes. The content of chlorophyll in the leafs of the plant is one of potential productivity indicators. It is often used aiming to find how any of the growing ways or environmental conditions affect the photosynthesis system of the plants. If growing conditions are inadequate, concentration of chlorophylls and ratio of chlorophylls a to b decreases. For the process of photosynthesis chlorophyll a is more important as it reacts to changing conditions of the environment rather [64,65]. Our data found differences between a chlorophyll content in the leafs ot tomatoes grown in different substrates. The smallest content was in the leaves of tomatoes grown in peat + zeolite (15%) substrate. Tomatoes grown in peat + zeolite (30%) substrate and peat only accumulated the same content of chlorophylls. Highest ratio of chlorophylls a to b was in the leaves of tomatoes grown in peat substrate. To sum up, positive impact of zeolite for synthesis of pigments of photosynthesis was not found.
Most researches found mixing of zeolite into other substrate has an influence on the quality of fruits. Aghdak et al.  in a study based on the effect of various substrates on qualitative properties of sweet pepper found that addition of zeolite to substrate improves quality of sweet pepper fruits. According to Angelis and other  results tomato fruit quality was affected only by tomato variety and not by substrate. Other data showed that no significant differences were found between type of substrate on the amount of total soluble solids, sugars and ascorbic acid in fruits of tomatoes [43,55]. Fecondini et al.  data showed hybrid but not the substrate plants were grown in had bigger impact for the yield of tomatoes. Our analysis showed that the content of titratable acidity was bigger in the fruits of tomatoes grown in peat-zeolite substrates.
According to different researchers, the admixture of zeolite into a peat substrate has positive influence on the yield of vegetables [14,68]. Ashraf  states, after admixture of zeolite into perlite and pumice not only the features of substrate improves but also the yield of tomatoes increases. Jankauskienė at al.  data show, growing of seedlings in peat-zeolite substrate had an influence on the quality of the seedlings though it did not have positive influence on the yield of these vegetables. It is important ratio of zeolite and other substrates, its size of fractions [23,71]. Berar et al.  data show after admixturing 25% zeolite into substrate there was found the biggest yield of tomatoes. Živković and others  reported admixturing zeolite into peat there was found 35% bigger yield of tomatoes, though the yield in the trials after admixturing 20% and 30% zeolite was the same. Cativelo  found more suitable substrate for growing of plants is admixtured substrate with 3-7% of zeolite compared to substrate which has 15% zeolite. Roses were grown in zeolite and perlite substrates mixed in different ratio. The biggest number of roses was picked and its quality was the best when roses were grown in zeolite and perlite substrate when ratio was 25 : 75 . Our data showed smaller amount of zeolite in peat (15%) had bigger impact for yield of tomatoes and average mass of fruit. After admixturing bigger amount of zeolite into peat substrate (30%), the yield of tomatoes was not bigger but in the leafs of tomatoes there were accumulated more dry matters.
The content of dry matter and photosynthetic pigments in tomato leaves depended on the substrate: the tomatoes grown in rockwool accumulated higher dry matter and chlorophylls in leaves than those of tomatoes grown in coconut fiber substrate. The intensity of photosynthesis depended on the hybrid of tomato. Photosynthesis intensity of tomato hybrid ‘Admiro’ was more intensive in rockwool and photosynthesis intensity of hybrid ‘Raissa’ – in coconut fiber substrate. Tomato transpiration was intensive in both substrates, but small stem increase in 24 hours showed insufficient supply of water for plants. This delayed fruit growth in day, especially of these tomatoes, which were grown in coconut fiber substrate. The yield of tomatoes grown in coconut fiber substrate was bigger than this one of tomatoes grown in rockwool. The ascorbic acid content in tomatoes fruit which were grown in coconut fiber was higher than in fruit of tomatoes grown in rockwool.
Plants grown in peat-zeolite substrates were lower. The admixture of zeolite into a peat substrate did not influence significantly dry matter accumulation in tomato leaves. The highest chlorophyll a to b ratio was in the leaves of tomatoes grown in peat substrate. Thus, the positive effect the admixtures of zeolite into peat substrate on synthesis of photosynthetic pigments were observed.
Admixture of zeolite into peat substrate affected the volume of the yield and the average fruit mass. The yield of tomatoes grown in peat + zeolite (15%) substrate was the highest. The tomatoes grown in peat + zeolite (15%) substrate recorded the highest average fruit mass. The tomatoes grown in peat-zeolite substrates accumulated less sugar, ascorbic acid, soluble solids, however, higher amount of titratable acidity and carotenoids.
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