The photoacoustic method allows direct determination of the energy-storage efficiency of photosynthesis by relating the energy stored by it to the total light energy absorbed by the plant material (Canaani et al., 1988; Malkin & Cahen, 1979; Malkin et al., 1990). These authors applied the photoacoustic method to leaves in the gas phase, where brief pulses caused concomitant pulses of oxygen that caused a pressure transient detected by a microphone. This method is based on the conversion of absorbed light to heat. Depending on the efficiency of the photosynthetic system, a variable fraction of the absorbed light energy is stored, thereby affecting the heat evolved and the resulting photoacoustic signal. The higher the photosynthetic efficiency, the greater will be the difference between the stored energy with and without ongoing photosynthesis (Cha & Mauzerall, 1992). These authors collected microalgal cells onto a filter and studied them by an approach similar to that previously used with leaves. In both cases, the oxygen signal is combined with that of thermal expansion resulting from conversion of the fraction of the light energy in the pulse that is not stored by photochemistry.
In the case of liquid algal cultures, there is no signal due to photosynthetic oxygen evolution as gas; hence, the signal detectable by an immersed microphone is proportional to the heat generated by a laser pulse. The light absorbed by the photosynthetic pigments in the algal cells is, in part, stored by photochemistry as products of photosynthesis, while the remainder is converted to heat, causing an expansion of the culture medium. This expansion causes a pressure wave that propagates through the culture and is sensed by the hydrophone. By exposing the cells to continuous saturating background light, no storage of any of the pulse energy can take place, whereas in the absence of such light, a maximal fraction of the pulse energy is stored by photosynthesis. Thus, the maximal photosynthetic storage efficiency, PSmax, is determined from the difference between the signal obtained from a weak laser pulse under strong, continuous illumination (PAsat) and that obtained in the dark (PAdark). The above is then divided by PAsat.
[For development of equations, see Cha and Mauzerall (1992)].
The experimental setup is shown schematically in Figure 1. The sample was placed in a sample cell. The beam of the brief laser pulse (5ns) is incident upon the suspension of algae, whose pigments absorb part of the laser light (Fig. 2). Depending on the experimental conditions, a variable fraction of the absorbed light pulse is stored in the products of photosynthesis. The remainder of the absorbed light is converted to heat, which causes a transient expansion of the surrounding water, producing an acoustic wave. This is intercepted by a submerged microphone containing a pressure-sensing ceramic disc.
A small portion of the laser pulse is used to trigger the Tektronix TDS 430A oscilloscope, where the amplified (Amptek A-250 Preamp and Stanford Research 560 Amp) photoacoustic signal is recorded (computer). An amount of 10 μs of the time scale was found by us as the optimal duration for quantification of the signal, beyond which the signal to noise ratio deteriorates. This time frame allowed us to fire the laser at 10 hz, thus averaging 128-256 pulses.
2. Quantification of biomass
Biomass detection by photoacoustics is based on the proportionality of the absorbed light to the amount of pigment (Dubinsky et al., 1998; Mauzerall et al., 1998). At high energies, the pulse saturates photosynthesis, and the photosynthetically stored energy becomes a negligible fraction of the absorbed energy. Under these conditions, the photoacoustic signal was proportional to the concentration of chlorophyll over the range of 14 mg to 8.5 μg chl a m -3 (Fig. 2) (Dubinsky et al., 1998; Mauzerall et al., 1998). Figure 3 shows the photoacoustic signal of a
3. Energy storage in photosynthesis
In a photosynthetic system at a given constant light intensity, a fraction of the reaction centers is closed at any time and only part of the light energy is stored (Dubinsky et al., 1998; Mauzerall et al., 1998). Figure 4 shows the photoacoustic signal in the dark (broken line) and saturating light conditions in a suspension of
By increasing the continuous background light intensity from zero to saturation of photosynthesis, an increasing fraction of the reaction centers is closed at any time, and a decreasing fraction of the probe laser pulse energy is stored. A corresponding increase in the fraction of the pulse energy in converted into heat and sensed by the photoacoustic detector. When all reaction centers are saturated, all the probe pulse energy is converted into heat (Fig. 4).
4. Demonstration of applications
We were able to follow the effects of the key environmental parameter, nutrient status, on the photosynthetic activity of phytoplankton and macroalgae. The nutrients examined were iron (Pinchasov et al., 2005), nitrogen, and phosphorus (Pinchasov-Grinblat et al., 2012).
5. Iron limitation
Three algal species, the diatom
Each culture was diluted in the corresponding medium to chlorophyll
The photoacoustic experiments were conducted after two weeks in these media. As the iron was progressively depleted, the ability of the three species to store energy decreased (Fig. 5). As seen in Figure 5, all three algal species showed a sharp decrease in efficiency.
Three species of marine phytoplankton,
In these experiments, the photoacclimation of the three algal species,
7. Lead exposure
In our experiments, the exposure of the cyanobacterium
With an increasing lead concentration and duration of exposure, the inhibition of photosynthesis increases. Since the photoacoustic method yields photosynthetic energy storage efficiency, the results are independent of chlorophyll concentration, which means that the observed decrease in efficiency is not due to the death of a fraction of the population, but rather due to the impairment of photosynthetic function in all cells, possibly due to the progressive inactivation of an increasing fraction of the photosynthetic units.
8. The effect of nutrient enrichment on seaweeds
Samples of the macroalga
The samples were exposed to 3 treatments: nitrogen (added as NaNO3 at a concentration of 3.25 gL-1), phosphorus (added as NaH2PO4 at a concentration of 0.025 gL-1), and nitrogen and phosphorus together. Controls were kept in unenriched seawater.
Nutrient limitation, on the one hand, and anthropogenic eutrophication, on the other, are among the most important factors determining the overall ecological status of water bodies. In general in all samples, photosynthetic efficiency and chlorophyll concentration (photoacoustic signal) decreased with time.
As is evident from Figure 8, macroalgae rapidly exhausted nutrients in the water, and within 190 h, the controls declined to approximately ~50% of the initial values in
Recently, Yan et al. (Yan et al., 2011), using a photoacoustic setup, measured thermal dissipation and energy storage in the intact cells of wild type
9. Other applications of photoacoustics
The thermal expansion of tissue, liquids, and gases due to light energy converted to heat, is termed the photothermal signal. This is always generated when photosynthetic tissue or cell is exposed to a light pulse, since plant tissue never absorbs all of the light stored as products of the process. The unused fraction of the absorbed light energy is converted to heat, resulting in measurable transient pressure (Cahen et al., 1980; Malkin, 1996). In addition to this thermal expansion signal, when a leaf is illuminated by a pulse of light, the resulting photosynthetic photolysis of water causes the evolution of a burst of gaseous oxygen. This process leads to an increase in pressure, a change which is readily detected by a microphone as the photobaric signal. For detailed definitions and description, see review by Malkin (1996).
The photoacoustic technique allows an investigation of energy conversion processes by photocalorimetry and direct measurement of the enthalpy change of photosynthetic reactions (Cahen & Garty, 1979; Malkin & Cahen, 1979). Oxygen evolution by leaf tissue can be measured photoacoustically with a time resolution that is difficult to achieve by other methods (Canaani et al., 1988; Cha & Mauzerall, 1992). A microphone can sense the photoacoustic waves via thermal expansion in the gas phase, thus allowing in-vivo measurements of the photosynthetic thermal efficiency and the optical cross section of the light harvesting systems. O2 evolution in intact undetached leaves of dark adapted seedlings was measured during photosynthesis with the objective to detect genetic differences among cultivars (da Silva et al., 1995).
The rapid response of the phytoplankton populations to changes in environmental factors, such as temperature, light, nutrients, vertical mixing, and pollution, necessitates simple and frequent measurements. The photoacoustic method provides unique capabilities for ecological monitoring, photosynthesis research, and the optimization of algal mass cultures, such as those designed for the production of biofuel, aquaculture feed, and fine chemicals.
The authors wish to thank L.P.P. Ltd. Publishers for permission to use Figure 5 (The effect of different iron concentrations on the relative photosynthetic efficiency in the three algae
This research was supported by European Research Council 2009 AdG – grant 249930 to Z.D. and by EU FP7 European Research Council 2012 – grant 309646 to Z.D.
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