Neuroblastoma (NB) is a childhood neoplasm and the cause of ~15% of cancer deaths in children. The clinical behavior of NB is highly variable. While some tumors are easily treatable, nearly 50% of the tumors exhibit very aggressive behavior. The latter tumors are classified as high-risk NB and are characterized by widespread tumor dissemination and poor long-term survival. Determining the prognosis of NBs at the time of diagnosis is important because of the clinical heterogeneity of the disease. Current prognostic factors used by the COG (Children's Oncology Group) Neuroblastoma Study for patient stratification and protocol assignment include: Age (<18 months
Current treatment for high-risk NB includes high dosage cytotoxic chemotherapy or myeloablative cytotoxic therapy with autologous hematopoietic stem cell transplantation . Late relapse is often seen in patients with high-risk NB despite achieving a complete clinical remission. A subset of high-risk NBs, which is refractory to current front-line therapy designed for high-risk NB, is termed ultra high-risk NB [8, 9]. These tumors are totally unresponsive to current therapies, and thus reliable diagnostic tools to identify ultra high-risk NB prior to treatment and innovative and effective therapeutic agents against these NBs are in need of development.
In this article, we will discuss our recent study on neuroblastoma stem cells, histopathological characteristics of these cells, and why the knowledge gained would help improve diagnosis and treatment of children with the most malignant NBs. We have recently reported the establishment of phenotypically stabilized stem cell-like NB cells (refer to as iCSC, see below) by short-term treatments of conventional monolayer NB cell lines with epigenetic modifiers . The study addresses a fundamental problem that has affected a complete success in treating patients with cancers. Cancer stem cells (CSCs) are plastic in nature, a characteristic that hampers cancer therapeutics. To date, two models have been proposed to explain the existence of cancer stem cells in a tumor mass: the stochastic model and the hierarchical model. According to the stochastic model, transformed single cells develop unlimited proliferative capability to cause a tumor. Initially, a single or few transformed cells result in uncontrolled growth. Accumulations of different mutations then occur driving additional tumor growth and resulting in heterogeneous subpopulations within the tumor. These cancer cells are believed to participate in tumor growth, develop resistance, and cause recurrence. Hence, all cells are considered tumorigenic and are targets for treatment. In contrast, the hierarchical or current CSC model states that in a given tumor, there exists a population of cancer cells that have characteristics similar to stem cells. Cancer stem cells have the capacity to renew indefinitely, to initiate tumor formation, and to give rise to multiple non-tumorigenic progenies via asymmetric cell division. As a result of this phenotypic drift, an established tumor would always consist of a mixture of CSC and non-CSC. Current anti-cancer therapies are believed to target the more differentiated tumor cells, but not the CSC component, which is ultimately responsible for tumor recurrence. Based on the most current thinking, the two models are not mutually exclusive.
To create phenotypically stabilized stem cell-like NB cells, our approach includes a short-term treatment (i.e., five days) of NB monolayer cell lines (SKNAS, SKNBE(2)C, CHP134, SY5Y) with either an inhibitor of DNA methylation and/or an HDAC inhibitor followed by cell culturing in the sphere-forming medium without the epigenetic modifiers. This strategy not only significantly augments the expression of the Yamanaka reprogramming factors and stem cell markers in the NB spheres generated, but it also captures these spheres in the “totally undifferentiated status” over a long period of time
The stem cell-like NB cells that are created in our recent study are characterized by their high expression of stemness factors, stem cell markers, and their open chromatin structure. We referred to these cells as induced CSC (iCSC) . Our
As shown in Fig. 1, the SKNAS monolayer cell xenografts presented a mosaic pattern and were composed of at least two distinct components having different cellular morphologies. Tumor cells in the first component were larger cells. Tumor cells in the other component were smaller in both cellular and nuclear size, and had smaller nucleoli (Fig. 1, upper left panel). Furthermore, these small tumor cells in the second component had reduced activities of mitosis and karyorrhexis (either intermediate MKI of 100~200/5,000 cells or low MKI of <100/5,000 cells) and often produced neurites or neuropils (Fig. 1, lower left panel). In addition, these smaller cells do not express MYC (Fig. 2). The monolayer cell xenografts were thus classified as poorly differentiated NB. In contrast, the SKNAS iCSC xenografts were composed of a diffuse and solid growth of medium-sized, rather uniform cells with a large vesicular nucleus and one or few prominent nucleoli (Fig. 1 right panel). Mitotic and karyorrhectic activities were frequently encountered (either intermediate MKI of 100~200/5,000 cells or high MKI of >200/5,000 cells). The iCSC xenografts were thus classified as totally undifferentiated “large-cell” NB, according to the International Neuroblastoma Pathology Classification [2, 3, 23, 25]. In fact, as reported in our study, all of the other iCSC xenografts from SKNBE(2)C, CHP134, and SY5Y have the LCN phenotype . Fig. 3 shows a remarkable resemblance of SKNAS iCSC xenografts and human LCN histologically.
In contrast to the consistently high MYC/MYCN expression, among the NB xenografts examined, there is a differential expression of CXCR4 in the SKNAS iCSC xenografts over monolayer cell counterparts (Fig. 5). It should be mentioned that both the larger and smaller cells of the SKNAS monolayer cell xenografts described in Fig. 1 were negative for CXCR4 staining, except some rare cases where a few cells were focally positive for CXCR4 staining (Fig. 5). These observations suggest that the large cells in SKNAS iCSC xenografts had different molecular and biological characteristics from the larger cells in the monolayer cell xenografts. However, the pattern of CXCR4 expression observed among the SKNAS xenografts was not always seen among the other iCSCs. Xenografts from both iCSC and monolayer cells of SKNBE(2)C, CHP134, SY5Y were all positive for CXCR4, but the staining in these cases was not intense and uniform .
In conclusion, the xenografts established from the NB iCSCs shared two consistent and common features: the LCN phenotype and high-level MYC/MYCN expression. In addition, our observations suggest that NB cells with large and vesicular nuclei, representing their open chromatin structure, are indicative of stem cell-like tumor cells, and that epigenetic changes may have contributed to the development of these most malignant NB cells. These observations have significant clinical implications. Specifically, one may identify the most malignant and aggressive type of NBs that require immediate innovative therapeutic intervention by examining histological/cytological appearance of the tumor, namely totally undifferentiated large-cell NB with prominent nuclei and high-level expression of MYC and/or MYCN by immunohistochemical analysis. Finally, the availability of the NB iCSCs will serve as useful tools to develop effective anti-CSC agents for NB in vivo and will help improve treatment and cure for children with neuroblastoma.
Dr. Xao Tang is supported by grants from NIH CA97255, CA127571, and a grant from the St. Baldrick Foundation. We would like to acknowledge Dr. Naohiko Ikegaki for his significant contribution in the development and establishment of the iCSCs described in this study and Jonathan Harbert for his technical assistance with the immunohistochemistry analyses.
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