Temperature stress is becoming the major concern for plant scientists worldwide due to the changing climate. The difficulty of climate change is further added considering its precisely projecting potential agricultural impacts [1, 2]. Temperature stress has devastating effects on plant growth and metabolism, as these processes have optimum temperature limits in every plant species. Global climate change is making high temperature (HT) a critical factor for plant growth and productivity; HT is now considered to be one of the major abiotic stresses for restricting crop production . The US Environmental Protection Agency (EPA) indicates that global temperatures have risen during the last 30 years , and it was mentioned that the decade from 2000 to 2009 was the warmest ever recorded.
High temperature stress is defined as the rise in temperature beyond a critical threshold for a period of time sufficient to cause irreversible damage to plant growth and development . The growth and development of plants involves a countless number of biochemical reactions, all of which are sensitive to some degree to temperature . Consequently, plant responses to HT vary with the extent of the temperature increase, its duration, and the plant type. Worldwide, extensive agricultural losses are attributed to heat, often in combination with drought or other stresses .
Low temperature (LT) or cold stress is another major environmental factor that often affects plant growth and crop productivity and leads to substantial crop losses [8, 9]. Chilling stress results from temperatures cool enough to produce injury without forming ice crystals in plant tissues, whereas freezing stress results in ice formation within plant tissues. Plants differ in their tolerance to chilling (0-15°C) and freezing (<0°C) temperatures. Both chilling and freezing stresses are together termed low temperature or cold stress: the damage due to cold stress can range from chilling injury and freezing injury to suffocation and heaving. In general, plants from temperate climatic regions are considered to be chilling tolerant to variable degrees, and their freezing tolerance can be increased by exposing to cold, but non-freezing, temperatures; this process is known as cold acclimation. However, generally the plants of tropical and subtropical origins are sensitive to chilling stress and lack this mechanism of cold acclimation . Low temperature may affect several aspects of crop growth; viz
The cellular changes induced by either HT or LT include responses those lead to the excess accumulation of toxic compounds, especially reactive oxygen species (ROS). The end result of ROS accumulation is oxidative stress [10-12]. In response to HT, the reaction catalyzed by ribulose-1,5-bisphosphate carboxylase oxygenase (RuBisCO) can lead to the production of H2O2 as a consequence of increases in its oxygenase reactions . On the other hand, LT conditions can create an imbalance between light absorption and light use by inhibiting the activity of the Calvin–Benson cycle. Enhanced photosynthetic electron flux to O2 and over-reduction of the respiratory electron transport chain (ETC) can also result in ROS accumulation during chilling which causes oxidative stress . Plants have evolved a variety of responses to extreme temperatures those minimize damages and ensure the maintenance of cellular homeostasis . A considerable amount of works have explored that there is a direct link between ROS scavenging and plant stress tolerance under temperature extremes . Thus, the improvement of temperature stress tolerance is often related to enhanced activities of enzymes involved in antioxidant systems of plants. Plants exposed to extreme temperatures use several non-enzymatic and enzymatic antioxidants to cope with the harmful effects of oxidative stress; higher activities of antioxidant defense enzymes are correlated with higher stress tolerance. Different plant studies have revealed that enhancing antioxidant defense confers stress tolerance to either HT or LT stress [16-19].
In this chapter, we review the recent research findings those revealed variable responses of plants to extreme temperatures. We also focus on the oxidative stress and antioxidant defenses that are invoked by plants for survival under temperature stress conditions.
2. Plant responses to high temperature
2.1. Seed germination and emergence
Seed germination and seedling vigor are important traits for obtaining a good plant stand and subsequent high yields of a crop. Seed germination is highly dependent on temperature as temperature is one of the basic requisites of this process. However, the range of temperature in which seeds perform better germination depends largely on crop species (Table 1). Soil temperature is one of the major environmental factors that influences not only the proportion of germinated seeds, but also the rate of emergence and the subsequent establishment, even under optimum soil and irrigation conditions .
|Egg plant (||15||33||20-25|
|Water melon (||15||35||25-30|
Roberts  documented three distinct physiological processes during seed germination which are affected by temperature: ‘first, temperature, together with moisture content, determines the rate of deterioration in all seeds; second, temperature affects the rate of dormancy loss in dry seeds and the pattern of dormancy change in moist seeds; and, third, in non-dormant seeds, temperature determines the rate of germination’ (for review see ).
The effect of HT on germination was investigated in various crops and serious impacts of HT on seed germination were observed. Increasing temperature between base and optimum temperatures increased the rate of germination and total percentage germination, but temperatures above optimum decrease the germination percentage [Prasad et al. 2006]. Essemine et al.  observed that very HT (45°C) did not allow adequate rate of germination due to cell death and embryo damage in
2.2. Growth and morphology
The most observed effect of heat stress on plants is the retardation of growth. As heat stress often occurs simultaneously with drought stress, the combination of drought and heat stress induce more detrimental effect on growth and productivity of crops than when each stress was applied individually . In higher plants, heat stress significantly alters cell division and cell elongation rates which affect the leaf size and weight. However, it was reported that heat stress resulted in significant increases in leaf numbers, particularly when reproductive development was arrested without any decrease in leaf photosynthetic rates [20, 24]. Exposure of plants to severe heat stress decreased the stem growth resulting in decreased plant height . Rahman  reported that plant height of wheat plant ranges from 66.4-97.3 cm and 55.7-82.3 cm in normal and heat stress condition, respectively. While studying with
High temperature decreased shoot dry weight, relative growth rate (RGR) and net assimilation rate (NAR) in maize, millet and sugarcane [5, 37]. In their review, Wahid  mentioned that HT can cause considerable pre- and post-harvest damages, including scorching of leaves and twigs, sunburns on leaves, branches and stems, leaf senescence and abscission, shoot and root growth inhibition, fruit discoloration and damage. High temperature also alters the internal morphology (anatomy) of plants and these changes are generally similar to those under drought stress. Under HT stress, there is a general tendency towards reduced cell size, closure of stomata and curtailed water loss, increased stomatal density and trichomatous densities, and larger xylem vessels in both roots and shoots . Several lines of study indicate that exposure of plants to HT resultes in the disintegration of ultrastructural characteristics, mainly attributed to a lower stomatal density, larger stomatal chamber with a larger stomatal opening area, thinner leaves, loose arrangement of mesophyll cells, a partially developed vascular bundle and unstable organelle structure. Zhang et al.  examined the microscopic and ultrastructural characteristics of mesophyll cells in flag leaves of both HT sensitive and tolerant rice genotypes grown under heat stress (37/30°C) and reported that the membrane permeability increased in both sensitive and tolerant plants under HT stress. However, under the HT stress, the tolerant plants showed tightly arranged mesophyll cells in flag leaves, fully developed vascular bundles and some closed stomata, whereas the sensitive plants suffered from injury because of the poor structures of these organs . Recently, Johkan et al.  observed that the number of tillers in wheat plants decreased in response to HT, especially high night-time temperatures, however shoot elongation was promoted.
2.3. Physiological effects
Physiological processes of plants are largely affected by the alteration of surrounded environmental temperature. The ability of plants to cope with extreme temperature is a complex process and is determined by environmental factors and also by the genetic capability of the plant. In general, stability of life processes in most plants is comparatively wide which ranges from several degrees above zero to around 35°C . The increase of temperature up to a certain level increases plant growth, photosynthesis, respiration and enzyme activity and after that these parameters tend to decline (Fig. 1). Respiration rapidly increases with temperature and drops drastically after an extreme tolerable temperature. Photosynthesis is a comparatively less sensitive than respiration process but its declining pattern is as like as respiration. The average rate of enzymatic reactions increases twofold with every 10°C increase in temperature within the range. The optimal temperature for structural integrity and activity of most enzymes are within the range of 30–45°C; and enzymes are irreversibly denatured and inactivated at temperatures higher than 60°C with the exception of thermophilous organisms. Thus each life process has its own referred critical or lethal temperature after that it can not proceeded and causes permanent damage to cell structures and ultimately the cell, plant death as well .
Temperature plays one of the most important roles in the rate and ability of a plant to photosynthesize effectively. In general, there is a positive correlation between change in temperature and photosynthesis. But when temperatures exceed the normal growing range (15°C to 45°C) of plants heat injury takes place and HT hurts the enzymes responsible for photosynthesis. Even in the absence of heat stress injury, photosynthesis would be expected to decline as temperature increases because photorespiration increases with temperature faster than does photosynthesis .
In tobacco leaves HT stress (43°C for 2 h) decreased the rate of photosynthesis by 38% compared with that of the rate of photosynthesis at optimal temperature (25°C). After 1 d recovery, it reached only about 75% of its control. Under HT condition, the stomatal conductance (
It was noted that an increase in temperature of 10°C to 15°C above normal growth temperature leads to alteration of photosynthetic pigments and thus limiting photosynthesis. The reasons for decreasing in photosynthetic pigments under HT may be attributed to the inhibition of biosynthesis, changes in ultrastructure of chloroplast, especially the membrane, and photodeterioration [47, 48]. Tewari and Tripathy  observed that heat stress significantly reduces Chl content in
2.5. Water relations
Plant water status is considered as the most important variable under changing ambient temperatures . Plant water relation is more affected under the combined heat and drought stress, than the condition of heat and sufficient moisture level. High temperatures affect seedlings, first, by increasing evaporative demand and tissue damage. High temperatures-induced increased transpiration and water transportation is another necessary tool for plant survival under extreme temperatures. Death of a large number of
2.6. Dry matter partitioning
Dry matter (DM) partitioning varied widely under different temperatures and crops. Stresses like water deficit and heat slower down the assimilation process and the mineral uptake during the grain filling period. Assimilates those are transferred directly to kernels and remobilization of assimilates stored in vegetative plant parts both together contribute to grain yield . Sometimes under HT, it happens that the sink activity lost due to the earlier panicle senescence where the source activity still exists as the leaf senescence does not occurs [63, 64]. In those cases, grain filling was terminated earlier than complete leaf senescence. Kim et al.  reported that consistently HT increased the rates of grain filling, fraction of DM partitioning to panicle and leaf senescence while it reduced the durations of them under the temperature regime of 24.4 and 21.9°C in temperate variety of
2.7. Reproductive development
It is notable that reproductive development of plants is more sensitive to HT because plant fertility is considerably reduced as temperatures increase . For heat-sensitive plants such as tomato, no fruit set occurs at day/night temperatures of 35/23°C . Studies on common bean , and peach  showed that elevated temperatures during flower development can markedly reduce the fruit set. The decrease in the fruit set has generally been attributed to low pollen viability and germinability at HT in crop species such as tomato  and groundnut . Porch and Jahn  reported that
The diurnal variation of temperature is also important for reproductive growth and development. Spikelet fertility of rice is sensitive to night temperature, where the degree of sensitivity depends upon the developmental stage of the spikelet . Later, Peng et al.  observed a strong negative linear relationship between the number of fertile spikelets and increase in night temperatures. Ledesma et al.  examined the effect of two day/night temperature regimes (30/25°C and 23/18°C) on fruit set and fruit growth in two strawberry cultivars (Nyoho and Toyonoka). It was recorded that high day/night temperature of 30/25°C reduced the number of inflorescences, flowers, and fruits in both cultivars compared with control (23/18°C). The percentage of fruit set in Nyoho was not significantly different between the two temperature treatments, while in Toyonoka it was much lower at 30/25°C than at 23/18°C. Ripening time was shorter at 30/25°C than at 23/18°C in both cultivars.
As HT negatively affected plant establishment, growth, DM partitioning, reproductive growth and photosynthesis, it ultimately poses serious consequence on crop yield. Several lines of study indicated the reduction of crop yield under HT which greatly varies with the degree and duration of temperature as well as genotypes of the crop (Table 2). Mendham and Salsbury  reported that HT can reduce crop yield by affecting both source and sink for assimilates. The decrease in grain length and width of cereals was found to be associated with a reduction in the average endosperm cell area observed under high night temperature . In addition, cereals generally respond to HT through an increase in the rate of kernel growth, which lead to a decrease in the duration of DM accumulation . Kernel dry weight reduced from 79 to 95% in field conditions in B-73 inbred line of maize under heat stress . Shah and Paulsen  demonstrated that photosynthesis and leaf area, shoot, grain biomass and sugar contents of kernels rapidly decreased under HT. High temperature affected the endosperm development in maize and reduced grain yield during endosperm cell division . Prasad et al.  observed that an increase in temperature from 32/22°C to 36/26°C and 40/30°C decreased seed yield of sorghum by 10 and 99%, respectively. Djanaguiraman et al.  grew sorghum plants both under normal (32/22°C) and HT (40/30°C) and observed significant differences in yield attributes and yield. After 45 d of treatment, plant height, leaf dry weight, seed weight and total dry weight decreased by 22, 14, 53 and 36%, respectively compared to optimum temperature. Compared to other crops, wheat is the most sensitive to HT as the reproductive growth, especially grain filling is greatly facilitated by LT. Mohammed and Tarpley  found almost 90% less grain in the plants grown in high night temperature (32°C) compared to normal temperature (27°C). Plants grown under high night temperature showed 20% decrease in grain weight compared to plants grown under normal temperature. Johkan et al.  reported that HT resulted in more immature grains and decreased yields in
|35/16°C (day/night), 10 d; during flower and pod development||Pod plant–1: 53%|
Seed yield: 48%
|35/18°C, 10 d; during bud formation, flowering, and pod development||Main stem pods: 75%|
Seeds pod–1: 25%
Seed weight: 22%
|35/16°C (day/night), 10 d during early flowering and pod development||Seed weight: 40%|
Harvest index: 7%
|Increase in temperature from 32/22 to 36/26 and 40/30°C (day/night); from emergence to maturity||Seed yield: 10 and 99%, respectively|||
|5°C higher than optimum; from sowing to 60 DAS, 61-80 DAS and 81 DAS to maturity||Grain spike–1: 18%|
Grain weight: 19%
Grain yield plant–1: 46%
|40/30°C (day/night), 63 d||Seed weight plant–1: 53%|||
|35/30°C (day/night); at heading stage||Panicle weight: 7%|
Spikelet weight: 16%
|32°C (10 h night temperature; stating from 20 DAE until harvest)||Yield per plant–1: 90%|||
|34°C, 7 d; during grain filling stage||Panicles plant–1: 10%|
Grain yield plant–1: 39%
Harvest index: 30%
|29/23°C (day/night); from 7 DAT to edible maturity||No. of fruits plant–1: 28%|
Yield plant–1: 62%
Fruit length: 18%
Fruit diameter: 20%
|31/18°C (day/night); from heading to harvest maturity||Grain number spike–1: 50%|
Grain yield plant–1: 39%
|27°C during grain filling period||Grain weight: 4%|||
3. Plant responses to low temperature
Cold or LT stress comprises of chilling (<20oC) and freezing temperatures (<0oC) those hamper the plant growth and development in many ways. Chilling-sensitive plants exposed to LTs usually show water-stress symptoms due to decreased root hydraulic conductance and leaf water and turgor potentials . Cold stress effects on crop plant have been studied since long time in many economically important crops [97-100] among which some of are chilling sensitive and unable to survive cold temperatures. Low temperature affects the plants in every stage of life starting from germination up to maturity.
Chilling injury is a serious problem during germination and early seedling growth in many plant species. For instance, optimum temperature range for germination of rice seed lies between 20 and 35°C, and the temperature of 10°C is cited as the minimum critical value below which rice does not germinate . There are many reports on positive correlation between germination at LT and root development at an early stage; and between the germination and seedling establishment . Angadi et al.  observed that the number of days to 50% germination in
3.2. Growth and morphology
Low temperature stress inhibits various metabolic reactions thus preventing the expression of full genetic potential of plants which is expressed by different phenotypic symptoms . Some of the common LT injury in plants are reduced seedling growth, seedling discoloration, leaf yellowing, leaf whitening, white specks, white bands, withering after transplanting, a reduced rate of tillering, stunting and so on . According to Angadi et al. , temperatures below 10°C result in slower and reduced growth and premature stem elongation in
Generally, exposure to cold temperature affects crop growth and development in two ways concurrently. First, developmental events in the shoot apex are affected which directly determine the differentiation of the panicle and hence potential yield and spikelet fertility resulting in fewer grains. Second, photosynthesis is impaired which reduces growth and results in indirect yield loss because there is less carbohydrate available for grain production . The cellular structures and components are also damaged due to the cold which have been studied for a long time. The development process and ontogeny of the organelles may be disrupted by chilling stress . Nahar et al. [98, 99] observed cold stress induced morphological symptoms like stunted plant, bushy plants, early maturity, yellowing of leaves. Modifications of cellular components include swelling of mitochondria, plastids and thylakoid lamellae, vesiculation of thylakoid, accumulation of lipid droplets and eventually the disorganization of the entire plastid , reduction in ribosomal numbers, dilation of endoplasmic reticulum, vesiculation of cytoplasmic membranes, condensation of nuclear chromatin, invagination of plasmalemma and vacuolation of membranous vesicles [111, 113] were noticed during chilling stress. Without these in extreme cases, chilling results in accelerated senescence and eventually plant death .
3.3. Reproductive development
Reproductive phases of the life cycle of plants are more vulnerable to cold stress . The reproductive phase faced to LT stress is influenced diversely in its different sub-phases. During the development of male gametophyte LT causes disruption of meiosis, tapetal hypertrophy, stunted development of pollen grain, anther protein degradation, pollen sterility, pollen tube deformation. In female gametophyte development its effects are characterized by reduced style and ovary length, disruption of meiosis, reduced stigma receptivity, callose deposition in style, damage to embryo sac components, and arrest of the fertilization process. At flowering LT may cause delayed flowering, bud abscission, sterile or distorted flowers, while at grain filling the source-sink relation is altered, kernel filling rate is reduced and ultimately small sized, unfilled or aborted seeds are produced [109, 116]. Flower buds of
3.4. Cell membrane damage
Extreme temperature injuries caused either by cold or by HT first attack on the cell membrane. There are many studies where cellular membranes have been shown as the primary site of freezing injury in plants [121, 122]. Cell membrane is damaged in two ways viz. disruption of protein lipid structure, protein denaturation and precipitation of solutes that indulges the membrane permeability. Due to LT stress the fatty acids become unsaturated and the lipid protein ratios of the membrane become altered which ultimately affect the membrane fluidity and structure as well . The flexible liquid-crystalline phase is converted in to a solid gel phase, thereby affecting the cellular function in different ways, viz. increased membrane permeability increases ion leakage, allows the entrance of undesirable anions and cataions into the cell, obstructs the exchange of essential ions, hampers the osmosis and diffusion processes, etc. All the phenomena are responsible for disrupting cellular homeostasis . Conversion of cellular water into ice is a major reason for cell rupture in cold stress. At first the ice formation occurs in apoplast having low solute concentration, this creates a vapor pressure between cytoplasm and apoplast and results in the migration of unfrozen cytoplasmic or cytosol water to the apoplast. This water gives a pressure which is the cause of enlargement of existing ice crystals and the pressure towards the cell wall and cell membrane which leads to cell rupture [123, 124].
Low temperatures may disturb the key organs of photosynthesis, including chloroplast and thylakoid membranes, causes swelling of plastids and thylakoid lamellae, vesiculation of thylakoid, accumulation of lipid drops and ultimately disorganization of entire plastid [111, 113]. Low temperature also disrupts the systems including electron transport, carbon cycle metabolism and
3.6. Water and nutrients movements and uptakes
Cell membrane plays major roles in water and nutrient movements within and outwards the cell. Intra- and extracellular water and nutrient movement are inhibited due to LT due to memebrane damage under LT. There can be of two types of abnormalities during LT stress. Cold damages the membrane that makes the membrane permeable to undesired nutrients and ions and causes ion leakage; another is cell membrane and cell wall can be ruptured by the cold which is also responsible for disrupting cellular homeostasis by destroying both intra and extracellular nutrient and water movements [132, 133]. Severe dehydration may also occur due to freezing of cell constituents, solutes and water . Available literature states that when temperatures drop below 0°C, the ice formation generally begins in the intracellular spaces because the intracellular fluid has a higher freezing point as compared to the other suborganales of cell [134, 135]. Low and freezing temperatures also lead to cellular dehydration, reduce water and nutrient uptake and conduction by the roots in some plants, thus causing osmotic stress . Yadav  stated that dehydration during cold occurs mainly due to reduction in water uptake by roots and a hindrance to closure of stomata. The success or failure of a seedling in the field is strongly related to the development of its root system under cold stress . In root of cucumber it was found that chilling caused injury to the cortical cells and further long time exposure increased the density of cytoplasm and damage the endoplasmic reticulum . Chilling-sensitive plants exposed to LT usually show water-stress symptoms due to decreased root hydraulic conductance; and decreased leaf water and turgor potentials . Freezing-induced increase in water viscosity is partly accounted for an initial decrease in root hydraulic conductance . During cold stress another phenomenon is common with the imbalanced water movement. The metabolic functions are altered those include production of more enzymes, isozymes though they help in maintaining more catalytic activity to cope with the LT stress .
3.7. Yield components and yield
Reproductive phase products are the key components of economic yield and hence LT stress during the reproductive phase has significant economic and social consequences. All the adverse effects of cold stress ultimately lower the yield of crop. Low temperature-induced yield reduction is a common phenomenon in many crops [98, 99, 140, 141]. Low temperature often causes flower abortion, pollen and ovule infertility, breakdown of fertilization, poor seed filling, decreases in seed setting which ultimately reduce the grain yield . In
4. Responses of perennial crops to extreme temperature
Like annual crops, perennial crops are also sensitive to extreme temperature. Fruits and nut trees are important crop plants which often face extreme temperature stress induced damages. Every fruit tree species has a range of optimum temperatures (Table 3) above or below which the growth and yield markedly reduced. The mean temperatures range for optimum growth of most tropical fruits are about 24-30°C [144, 145]. For instance, mango (
5. Oxidative stress under extreme temperature
Oxidative stress has been mentioned as a common metabolic route of different stresses , and the regulation of oxidative stress has been mentioned as an indication of abiotic stress tolerance of plants by different studies [3, 161-163]. Several recent reports indicated that under abiotic stress production of free radicles or ROS markedly increased [3, 164-166]. Temperature stress accelerates the generation of ROS including singlet oxygen (1O2), superoxide radical (O2•–), hydrogen peroxide (H2O2) and hydroxyl radical (OH•), thereby induced oxidative stress [10, 167]. In plant cells, ROS are continuously produced as a consequence of aerobic metabolism in all the intracellular organelles, particularly in the chloroplast, mitochondria and peroxisomes [160, 168; Fig. 4]. But the chloroplast is considered as the main source of ROS in plants.
Superoxide radical (O2●–) is formed in many photooxidation reactions (flavoprotein, redox cycling), Mehler reaction in chloroplasts, mitochondrial ETCs reactions, glyoxisomal photorespiration, NADPH oxidase in plasma membranes and xanthine oxidase and membrane polypeptides. Hydroxyl radical (OH●) is formed due to the reaction of H2O2 with O2●– (Haber-Weiss reaction), reactions of H2O2 with Fe2+ (Fenton reaction) and decomposition of O3 in apoplastic space [169, 170]. Hydroxyl radicals (OH●) can potentially react with all biomolecules like, pigments, proteins, lipids and DNA, and almost with all constituent of cells. Hydroxyl radical is not considered to have signaling function although the products of its reactions can elicit signaling responses, and cells sequester the catalytic metals to metallochaperones efficiently avoiding OH● [169, 170]. Singlet oxygen (1O2) is formed during photoinhibition, and PS II electron transfer reactions in chloroplasts. This radical directly oxidizes protein, polyunsaturated fatty acids, and DNA [171, 172].
The main effects of ROS include autocatalytic peroxidation of membrane lipids and pigments, modification of membrane permeability and functions [3, 173]. During the time of temperature stress, ROS level can increase dramatically which can result in significant damage to cell structure . Vallelian-Bindschedler et al.  reported that even very short heat pulses can result in oxidative bursts of O2•– and/or H2O2. Heat stress may disturb the homeostatic balance of cell and promote lipid peroxidation, either by increasing the production of reactive oxygen species or by decreasing the O2 radical scavenging ability in the cell . The drastic increase in lipid peroxidation due to HT stress was reported by many researchers [162, 177]. Several lines of study indicated that under heat-stress conditions, malondialdehyde (MDA), a product of peroxidation of unsaturated fatty acids, has been used as a good indicator of free radical damage to cell membranes [12, 178, 179]. In wheat seedlings (8-d old) gradual increase in the accumulation of H2O2 was observed (0.5, 0.58, 0.78 and 1.1 μmol g–1 FW) in response to differential heat shock treatment of 22, 30, 35 and 40°C for 2 h . The effect of a long-term (24 h) HT (42°C) shock on oxidative damages in
Low temperature is also responsible for the production of ROS in plant cell [183, 184]. In extreme cold beyond the plants tolerant level or in chilling sensitive plants the activities of antioxidant enzymes are reduced which accelerate the accumulation of ROS in higher amount. Production of ROS severely affects electron transfer and biochemical reactions [12, 108]. Low temperature-induced oxidative stress decreases phospholipid content, increases lipid peroxidation, free and saturated fatty acid content [185-187]. This stress damages lipid, protein, carbohydrate and DNA [Gill SS and Tuteja 2010], thus it alters the enzyme activities, biochemical reactions and plant physiological processes including photosynthesis, respiration, nutrient movements, transpiration which negatively affect plants survival. In extreme cases ROS induced oxidative stress causes cell death .
However, recent studies have shown that ROS could also play a key role in mediating important signal transduction events. The rates of ROS production during temperature stress could play a central role in stress perception and protection .
6. Antioxidant defense under temperature stress
Plants have various enzymatic and non-enzymatic defense systems to minimize the deleterious effects of ROS which include the enzymes: catalase (CAT), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione
Under HT stress, plants are found to accumulate enhanced amount of non-enzymatic antioxidant and upregulate the activities of antioxidant enzymes. However, in most of the cases these enhanced activities are not sufficient for stress tolerance in plants, especially in susceptible genotypes [3, 18]. Almeselmani et al.  observed that the activities of SOD, APX, CAT, GR and POX were increased significantly at all stages of growth in heat-tolerant cultivars (C 306) in response to heat stress while the susceptible cultivar (PBW 343) showed a significant reduction in CAT, GR and POX activities. While investigating the induction of antioxidant enzymes (SOD, APX, GR and CAT) in wheat shoot under HT stresses, Badawi et al.  observed that three wheat genotypes (Fang, Siete Cerros and Imam) showed differences in their antioxidant enzyme activities. Importantly, Fang, the heat tolerant genotype, showed higher SOD, APX, GR and CAT activities under HT stress compared to the other two genotypes which indicated the role of the antioxidant defense system in conferring heat stress tolerance. Djanaguiraman et al.  observed that HT stress decreased antioxidant enzyme activities and increased oxidant production in sorghum. In their study, SOD, CAT and POX activities were decreased in heat stress (22, 15 and 25% lower than control plants) and the greater inhibition of all antioxidant enzymes in heat-stressed plants relative to control plants indicates greater inactivation of all antioxidant enzymes by heat stress. On the other hand, the application of selenium (Se) decreased oxidative damages by enhancing antioxidant defense resulting in higher grain yield. In addition, the increase in antioxidant enzyme activities and decrease in ROS content by Se was greater in HT than in optimum temperature. In
A higher AsA content was found to associate with higher antioxidative capacity and higher cold tolerance in rice . Streb et al.  found to increase the contents of AsA and α-tocopherol in chilling-tolerant cereal leaves which helped to maintain better photosynthesis levels as compared to the chilling sensitive varieties. Fortunato et al.  stated that the elevated ROS production indicated by H2O2 and OH• was reduced by the over production of AsA and α-tocopherol contents under LT stress in
7. Conclusion and future perspectives
The extreme temperatures those are consequences of present-day global climate changes are considered as major abiotic stresses for crop plants. Different plant studies clearly show that temperatures exceeding the limits of adaptation substantially influence the metabolism, viability, physiology, and yield of many plants. Plants exposed to extreme temperatures often show a common response in the form of oxidative stress. However, the extent of damage caused by extreme temperatures depends greatly on the duration of the adverse temperature, the genotypes of the exposed plants, and their stage of growth. There is ample need to develop temperature tolerance in crop plants by exploring suitable strategies. Numerous research findings support the notion that induction and regulation of antioxidant defenses are necessary for obtaining substantial tolerance against environmental stress. The development of genetically engineered plants, by the introduction and/or overexpression of selected genes, would to be one feasible strategy. However, plant adaptation to either HT or LT is a multigenic response which is very complex in nature. Thus the task of identifying the traits those correlate with stress tolerance is incredibly difficult for researchers.
At present, number of genes have been identified in different studies but the knowledge of the transcriptional control of extreme temperature responses is limited. In addition, the regulation of these transcriptional responses is far more complex than previously believed. In recent years, a number of exogenous protectants, such as proline, glycinebetaine, nitric oxide, silicon, selenium, salicylic acid, and polyamines have been tested and found to be beneficial in protecting plants against damage from temperature extremes. Therefore, more advanced research should conduct focusing on the development of plants those restrain genes which promote the accumulation/synthesis of these beneficial elements and compounds. Considering these facts, a well organized approach should combine to investigate the molecular, physiological, and metabolic aspects of temperature stress tolerance both at the cellular and the whole plant level.