Open access

Application of Differential Scanning Calorimetry to the Characterization of Biopolymers

Written By

Adriana Gregorova

Submitted: 21 November 2011 Published: 23 January 2013

DOI: 10.5772/53822

Chapter metrics overview

9,721 Chapter Downloads

View Full Metrics

1. Introduction

Generally, polymers can be classified according to their thermal and mechanical properties into thermoplastics, thermosets and elastomers. Thermoplastics are amorphous or semi-crystalline polymers that soft or melt during heating and solidify during cooling. The heating/cooling/heating process can be repeated without perceptible changes in thermal and mechanical properties of thermoplastics. Thermosets during heating undergo chemical changes and this process is irreversible. Elastomers can be vulcanized (cross-linked under assistance of heat, light, or special chemicals like sulfur, peroxides) that makes them reversibly stretchable for small deformations but vulcanization is the irreversible process.

The resulted properties of polymer materials and mixtures depend on the chemical and physical properties of neat polymers, additives as well as the used processing methodology. Differential scanning calorimetry (DSC) is a physical characterization method used to study thermal behavior of neat polymers, copolymers, polymer blends and composites. Generally, the non-isothermal DSC is used for the identification of neat basic polymers as well as the determination of their purity and stability. Amorphous polymers exhibit a glass transition temperature and semi-crystalline polymers may possess the glass transition temperature, a crystallization temperature, a melting temperature with various crystallization and melting enthalpies. However, these properties alter by both a presence of additives and applied polymer processing methodologies. Basically, a small quantity of sample (up to 10 mg) in pan from various materials (e.g. aluminum pan) and empty pan (reference) are treated under a defined temperature program (various combinations of thermal scans-heating/cooling, and isothermal cycles), a pressure (stable) and an atmosphere (inert or reactive). Principally, sample and reference are maintained at the same temperature, while any transition occurred in the sample needs an energy supply, which is recorded by the DSC as a rate dQ/dt against a temperature or a time. The DSC is the thermal analysis mainly used to determine a first-order transition (melting) and a second order endothermic transition (glass transition). The sudden change in the specific heat value, Cp corresponds with the glass transition temperature as follows (Bower, 2002):


where m is the mass of the sample.

However, the determination of the glass transition of polymers with a high crystallinity content is limited. The first-order transitions such as the crystallization of a polymer during a heating (cold crystallization) or a cooling cycle (crystallization) and a melting of polymer crystals can be described by the following formula (Bower, 2002):


where κ is a thermal conductance between a sample holder and a sample, Tis a temperature increase rate, and t0 is the start of transition.

Figure 1 shows the example of thermal transitions occurring in the injection molded sample of poly(lactic acid) (PLA) such as the glass transition, the cold crystallization and the melting. PLA is a thermoplastic aliphatic semi-crystalline biodegradable polyester. The presented molded sample had been cooled very rapidly during the processing (injection molding), so as the consequence during the second heating cycle appeared the cold crystallization peak.

Figure 1.

DSC thermogram of commercial poly(lactic acid) with Mw¯= 70 400 and PDI = 1.8 detected during 2nd heating cycle (0-180°C, 10°C/min, N2 atmosphere)

There are two types of DSC systems: 1) heat-flux (sample and reference pans are in an identical furnace block) and 2) power compensation (sample and reference pans are in two separate furnace blocks). From the practical point of view, it is important to pay attention to issues influencing an accuracy of results as follows:

  • an instrument calibration, baseline subtractions,

  • a selection of working gas (N2, He, O2),

  • a selection of pans (e.g. Al-, Pt-, Ni-, Cu-, Quartz-pans, hermetic or non-hermetic pans),

  • a proper thermal contact between sample and pans,

  • a temperature program (heating cycle usually should start about 50°C under and finish about 10-20°C above the expected measured transition temperature),

  • a sufficient slow scanning rate (to avoid the neglecting of the requested thermal transition),

  • a sufficient purity and source of sample (neat polymer, polymer blend, composite, before or after processing, kind of the processing).

The aim of this chapter is to show some examples of the practical use of the DSC within the investigation of an amorphous biopolymer – lignin and semi-crystalline biodegradable polymer – poly(lactic acid) as well as to discuss the dependence of the thermal thermal properties on the value of the molecular weight of polymer, the polymer processing methodology and the presence of additives in the polymer mixtures.


2. Effect of molecular weight on glass transition temperature

Amorphous and semicrystalline polymers undergo a phase change from a glassy to rubbery stage at a glass transition temperature (Tg).

At Tg the segmental mobility of molecular chains increases and a polymer is more elastic and flexible. The value of Tg is dependent on the various factors such as a molecular weight of polymer, a presence of moisture, a presence of the crystalline phase (in the case of semicrystalline polymers). The dependence of Tg on a number-average molecular weight is described by Flory-Fox equation:


where Tgis a glass transition for polymer with the infinite number-average molecular weight, K is an empirical parameter related to the free volume in polymer and Mn is a number-average molecular weight of polymer.

2.1. Thermal properties of Kraft lignin extracted with organic solvents

In this sub-chapter, an example of the effect of various extraction solvents on molecular weight properties and thermal properties of Kraft lignin is shown.

Lignin is polydisperse amorphous natural polymer consisting of branched network phenylpropane units with phenolic, hydroxyl, methoxyl and carbonyl groups. Its molecular weight properties as well as functional groups depend on its genetic origin and used isolation method. Differential scanning calorimetry is the useful method to determine its glass transition temperature. The value of Tg depends on the molecular weight, the thermal treatment, the humidity content and the presence of various contaminants in lignin sample.

Generally, phenyl groups together with the cross-linking restrict the molecular motion of lignin as an amorphous polymer in contrast to propane chains. Moreover, the intermolecular hydrogen bonding decrease Tg in the contrast to the methoxyl groups (Hatakeyama & Hatakeyama, 2010). Lignin might be defined as a natural polymeric product produced by the enzymatic dehydrogenation polymerization of the primary methoxylated precursors such as p-coumaryl-, coniferyl- and sinapyl- alcohols (Figure 2).

Figure 2.

Lignin monomer building units

The structure of lignins depends on their natural origin and also on the external and internal conditions existing during lignin macromolecule synthesis and isolations. The large heterogeneity of lignin´s structures makes it difficult to determine the overall structure of lignin. High variability of substituents on phenyl propane unit together with auto-coupling reaction gives rise to different lignin´s structures depending on its origin and isolation method (Figure 3).

Figure 3.

Lignin isolation methods

Kraft lignin used in this study was isolated from commercial spent pulping black liquor through the acidification with 98% sulphuric acid to pH=2 (Zellstoff Pöls AG, Austria). Precipitated, filtered, washed and dried Kraft lignin was extracted at the room temperature with organic solvents with Hildebrand solubility parameters in the range of 18.5-29.7 MPa1/2 (see Table 1) and then again filtered and dried.

SolventChemical formulaHildebrand solubility parameter
Polarity index

Table 1.

Solvents used for Kraft lignin extraction

The determined thermal and molecular weight properties of Kraft lignins are shown in Table 2. The glass transition temperature (Tg) and the specific heat change (ΔCp) were assessed by the differential scanning calorimetry (DSC) under the nitrogen flow, using the second heating cycle. Molecular weight properties were determined by a gel permeation chromatography (GPC) with the using of tetrahydrofuran as an eluent.

Kraft lignin_acetone1140.086103018001.7
Kraft lignin_tetrahydrofuran1240.222117031502.7
Kraft lignin_dichlormethane590.2607509401.3
Kraft lignin_methanol1050.36891013001.4
Kraf lignin_1,4-dioxane1200.367115030702.7

Table 2.

Thermal and molecular weight properties of Kraft lignins extracted in acetone, tetrahydrofuran, dichlormethane, methanol and 1,4-dioxane

Figure 4.

DSC thermograms of Kraft lignin extracted in acetone, tetrahydrofuran, dichlormethane, methanol and 1,4-dioxane detected during second heating scan (5-180°C, 10°C/min, N2 atmosphere)

Figure 4 shows the thermograms of the individual Kraft lignins extracted with various organic solvents.

As can be seen from the results, the extraction as the last step used during the isolation process of Kraft lignin has a big effect on molecular as well as thermal properties of lignin.

2.2. Thermal properties of Poly(lactic acid) synthetized through azeotropic dehydration condensation

This sub-chapter shows the connection between PLA structure, its molecular weight properties and its thermal properties.

Poly(lactic acid) (PLA) is a biodegradable, thermoplastic, aliphatic polyester, which monomer can be derived from annually renewable resources. The glass transition temperature value is an important attribute that influences viscoelastic properties of PLA. The increase of the ambient temperature above Tg of PLA causes the sharp loss of its stiffness. The Tg values of PLA are influenced by its molecular weight, crystallinity, thermal history during processing, character of the side-chain groups and the presence of additives in the composition. The DSC analysis is one of the suitable methods to characterize the effect of the modification of PLA reactive side-chain groups on its thermal properties.

It is worth to mention that the melting temperature and the heat of fusion of polymers are influenced by thermal history applied during the polymer synthesis or processing. Therefore DSC results derived from 1st heating cycle give information concerning an actual state of polymer crystals and the application of cooling cycle erase the previous thermal history, e.g. annealing during processing. Some semi-crystalline polymers with the slow crystallization ability like poly(lactic acid) do not have time to crystallize during cooling and thus crystallize during 2nd heating cycle (cold crystallization) and consequently the melting peak may appear as double peak due to the content of different kinds of crystals. The melting behaviour of PLA is complex with regard to its multiple melting behaviour and polymorphism and has been intensively studied by several authors (Yasiniwa et al., 2004; Yasuniwa et al., 2006; Yasuniwa et al., 2007; Di Lorenzo, 2006).

PLA sample in the following example, marked as PLA 0, was synthetized by an azeotropic dehydration condensation in a refluxing boiling m-xylene from 80% L-lactic acid. During the azeotropic dehydration condensation samples PLA_1-3 were modified by succinic anhydride in the concentration 0.7, 1.3 and 2.5 mol% (Gregorova et al., 2011a). Table 3 summarizes the nomenclature and molecular properties of non-modified PLA and PLA modified with various concentration of succinic anhydride.

Figure 5 shows DSC heating/cooling/heating thermogram of non-modified PLA with the molecular weight of 35 600 g/mol.

Generally, glass transition temperature is determined from the second heating cycle to provide Tg value independent on the thermal history during processing. The modification of PLA side-chain groups by succinic anhydride influenced not just molecular weight properties of PLA but also their thermal properties such as the glass transition temperature (Tg), the melting temperature (Tm) (in this case Tm was determined as the peak temperature of the melting peak) and the crystallinity (see Figure 6. and Table 4). As an adequate indicator of the crystallinity was chosen the specific heat of fusion, calculated as follows:


where ΔHm1 and ΔHm2 are enthalpy values of the first and second melting peak, ΔHc is the enthalpy of cold crystallization.

SampleConcentration of succinic anhydride (mol%)Mn¯

Table 3.

Description of PLA samples and their molecular properties determined by GPC in chloroform

Figure 5.

DSC thermogram of PLA_0 detected during heating/cooling/heating scan (30-170°C, 170-0°C, -30-170°C, 10°C/min, N2 atmosphere)

By the comparison of the content of the crystalline phase determined from 1st heating and 2nd heating cycle, it can be seen that PLA samples during second heating cycle exhibit an amorphous character despite of the initially crystalline character determined from 1st heating scan. A thermal history is very important issue that influence the arrangement of amorphous/crystalline phase and consequently influence the physico-mechanical properties of poly(lactic acid).

Sample1st heating cycle2nd heating cycle

Table 4.

Thermal properties of PLA synthetized through the azetropic dehydration condensation from 80% L-Lactic acid and modified by succinic anhydride

Figure 6.

DSC thermograms of PLA samples with modified side chain groups and various molecular properties detected during second heating scan (-30-170°C, 10°C/min, N2 atmosphere)


3. Effect of thermal treatment on thermal behavior of poly(lactic acid)

As was already discussed in the previous sub-chapter, PLA is the semi-crystalline polymer with the slow crystallization ability. Mechanical properties as well as gas barrier properties of PLA depend also on its gained crystallinity value. The resulting crystallinity of PLA can be modified by a thermal treatment (annealing) for some time at the crystallization temperature during the thermal processing of a sample. The change of a crystals size and a form during the annealing can be revealed by a X-Ray analysis but the change in the percentage of crystalline phase is detectable also by the DSC analysis. This section describes the progress of the PLA crystalline phase due to the applied annealing treatment. Moreover, the obtained DSC data are supported by a light microscopy study.

The followed data were obtained by the analysis of the thermal compression molded poly(lactic acid) synthetized by the azeotropic dehydration condenstation (PLA_3) (Figure 7).

Figure 7.

Structure of PLA_3 (PLA synthesized by the azeotropic dehydration condensation and modified by 2.5 mol% succinic anhydride)

The crystallinity value of PLA was modified during thermoprocessing by the thermal annealing at 110°C for 0, 5, 10, 15, 20, 30, 45, 60 and 120 min, respectively and afterwards cooled down to the room temperature. The samples are designated as PLA_3_110_X, where X indicates annealing time.

The clear effect of the thermal annealing on the PLA melting behavior is shown in Figure 8.

Figure 8.

DSC thermograms of PLA_3 annealed at 110°C for 0-120 min (1st heating, 30-160°C, 10°C/min, N2 atmosphere)

The change of the annealing time influenced the value of the specific melting enthalpy (ΣΔH) due to the enabling of a growth of crystals (Table 5).

The crystals morphology of PLA samples annealed at 110°C and various times were investigated by using of the light microscope with crossed polarizers (Figure 9). It can be seen that a shape and dimensions of the created crystals depend on the annealing time.

The DSC as well as the light microscopy analyses showed that the thermo-processed films without the annealing processing step have an amorphous character (Figure 9a), and on other side the application of the annealing processing step at 110°C during thermoforming instead of a quick direct cooling step (to the room temperature) promotes the growth of crystals. A kind, a size, a thickness, and a content of arisen crystals depend on the annealing temperature and time. DSC data displayed in Table 5 showed that the value of the specific

Sample1st heating cycle
Tm1Peak height
Tm2Peak height

Table 5.

Thermal properties of PLA_3 films, annealed at 110°C for 0-120 min

Figure 9.

Polarized optical micrographs (magnification 400×) of crystals of polylactic acid modified with succinic anhydride (PLA_3) grown from the melt and annealed at 110°C for 5-120 min

heat of fusion markedly increased up to 15 min of the annealing time, but the extension of the annealing time up to 30 min increased ΣΔH just slightly and further extension of the annealing time even decreased it. However, light micrographs of PLA_3 (see Figure 9 b-i) show clear differences of the character of crystals, arisen from the samples annealed under and above 30 min. The application of the longer annealing time caused the creation of overgrowth crystals. The difference in the character of crystals can be also detected by the change of the height of the melting peak and by their shift to the higher temperatures. The value of ΣΔH of PLA annealed for 120 min (PLA_3_110_120) is comparable to that of annealed just for 10 min, however the crystal morphology is markedly different. Furthermore, the change of the crystal morphology was indicated by the increase of the melting temperature (Tm1 and Tm2) about 10 and 3°C, respectively. Also the optical micrograph displayed in Figure 9i showed the difference in the crystal morphology in a comparison to the previous samples annealed at the lower time. As a remark can be highlighted that the crystal morphology has an essential influence on resulting physico-mechanical properties of PLA materials.


4. Thermal stability of biopolymers determined by DSC

4.1. Effect of functional end groups on poly(lactic acid) stability

The intramolecular transesterification with the formation of cyclic oligomers and by-products like acrylic acid, carbon oxide and acetaldehyde is considered as one of the main mechanisms of the PLA thermal degradation. Above 200°C five reaction pathways have been found: intra-and intermolecular ester exchange, cis-elimination, radical and concerted nonradical reactions, radical reactions and Sn-catalyzed depolymerisation (Kopinke et al., 1996). It has been suggested that CH groups of the main chain and the character of functional end groups affect thermal and hydrolytic sensitivity of PLA (Lee et al., 2001; Ramkumar & Bhattacharya, 1998). In our previous work it was shown that thermal sensitivity of PLA might be improved by the modification of its functional end groups (Gregorova et al., 2011a). This sub-chapter shows that the DSC analysis can be used to determine the thermal stability of poly(lactic acid).

Figure 10.

DSC curves of low molecular weight PLA synthetized by azeotropic dehydration condensation (PLA_0) and modified by 2.5 mol.% succinic anhydride (PLA_3), detected by 1st heating cycle from 30 to 350°C at heating rate of 10°C/min, in nitrogen flow.

The obtained DSC data, displayed in Figure 10, showed that the modification of low molecular weight PLA with succinic anhydride caused the decrease of its melting temperature and crystallinity. Furthermore, the detected values of the onset degradation temperature, the degradation temperature in peak and the enthalpy of degradation indicate the improvement of thermal stability, caused by the modification of hydroxyl functional end group by succinic anhydride.

4.2. Stabilizing effect of lignin used as filler for natural rubber

Natural rubber (NR) is highly unsaturated polymer exhibiting poor resistance to oxidation. For the inhibition of the degradation process during thermo-oxidation can be used stabilizers such as phenol and amine derived additives. NR for the production of vulcanized products is mixed with the number of the other compounding ingredients to obtain the desired properties of vulcanizates (e.g sulfur, accelerators, and filler). Lignin is biopolymer that can be used as an active filler for rubber. It was found that some lignins can play dual role in rubber compounds, influencing their mechanical properties as well as their stability [11].

The obtained data were obtained by using of vulcanizates based on natural rubber (NR) and filled with 0, 10, 20 and 30 phr of Björkman beech lignin (Mw= 2000, PDI= 1.2) (Kosikova et al., 2007). Samples are designated as NR_Lignin_X, where X presents concentration of lignin in phr (parts per hundred rubber).

Table 6 shows values of degradation temperature determined as the onset and the peak temperature in dependence on the lignin concentration in natural rubber vulcanizates. It can be seen that lignin used as filler exhibit also the stabilizing effect, while the best stabilizing effect was reached in the case of 20 phr presence of Björkman beech lignin.


Table 6.

DSC data evaluated from 1st heating cycle analysis (30-500°C, 10°C/min, air atmosphere) of vulcanizates based on natural rubber (NR) and NR filled with Björkman beech lignin (Kosikova et al., 2007)

4.3. Stabilizing effect of lignin used as additive in polypropylene

It was already reported that the lignin in the certain circumstances can support the biodegradability of polymer samples (Kosikova et al., 1993a; Kosikova et al., 1993b; Mikulasova&Kosikova, 1999). On the other side lignin with the important functional groups and the low molecular weight with the narrow polydispersity can be used as the stabilizer for polypropylene (Gregorova et al., 2005a). This section shows that DSC is the sensitive method able to determine the stabilizing effect of lignin in polypropylene.

The polypropylene samples, stabilized with Björkman beech lignin (Mw= 2000, PDI= 1.2), used in this example were thermal processed with the injection molding (Gregorova et al., 2005a). Figure 11 shows the change of the onset oxidation temperature (Tonset) recorded for polypropylene stabilized with lignin. Generally, additives should be compatible with polymer matrix to keep physico-mechanical properties on the desired level; therefore it is necessary to know the lowest active concentration of the additive. It can be seen that the studied Björkman beech lignin increased Tonset about 15-30°C depending on the used concentration. On the base of the obtained mechanical properties of polypropylene/lignin composites, 2 wt% of Björkman beech lignin was determined as the optimal concentration to stabilize polypropylene. It was shown that the higher concentration of non-modified lignin deteriorated the mechanical properties of polypropylene (Gregorova et al., 2005a, Gregorova et al., 2005b).

Figure 11.

Thermal stability of polypropylene expressed as onset degradation temperature (Tonset) in dependence on lignin concentration, heating scan 30 to 500°C, heating rate of 1, 3, 5, 7, 10 and 15 °C/min, oxygen flow (Gregorova et al., 2005a).


5. Thermal properties of poly(lactic acid) composites

The incorporation of filler in PLA may change its crystallization behaviour and consequently its thermal properties. Some filler, such as wood flour or wood fibers, promote the transcrystallization and thus modify crystalline morphology of PLA (Mathew et al.; 2005 Pilla et al., 2008; Matthew et al., 2006; Hrabalova et al. 2010). This section describes the ability of hydrothermally pretreated beech flour to support a nucleation of PLA. Moreover, the effect of quick cooling and thermal annealing during thermal processing of PLA films is recorded.

The sample used in this section were thermoplastic processed compounds of commercial poly(lactic acid) (PLA 7000D, NatureWorks LLC, USA) plasticized with 10 vol% of polyethylene glycol 1500 and filled with 30 wt% of hydrothermally pretreated beech flour (Gregorova et al., 2011b). Composite films were prepared by thermal molding in press at 160°C, 10 MPa for 5 min and by modification of cooling process were prepared two morphologies: amorphous (quick cooling) and semi-crystalline (thermal annealing at 100°C for 45 min). The samples are designated as pPLA_X_100_Y, where X indicates filler (0-no filler, WF- hydrothermally pretreated beech flour) and Y shows annealing time.

The thermal behavior of quenched and annealed PLA composites, investigated by differential scanning calorimetry (heating cycle from 20 to 180°C, 10°C/min, 60 ml/L nitrogen flow) is summarized in Table 11 and shows that both filler incorporation of wood flour and thermal annealing influenced melting behavior and crystallinity of PLA composites. Specific melting enthalpy as an indicator of crystallinity degree of PLA in the composite was calculated as follows:


where ΔHm1 and ΔHm2 are enthalpy values of the first and second melting peak, ΔHc is the enthalpy of the cold crystallization and ν is volume fraction of PLA in the composite.

1st heating cycle2nd heating cycle
ΣΔH (J/g)ΔHm1/ ΔHm2

Table 7.

DSC thermal data of non-annealed and annealed PLA composites determined (Gregorova et al.; 2011b)

Samples that were after melting quickly cooled down to room temperature (quenched) exhibit cold crystallization and the double melting behavior that may be attributed to the melting of the original crystals and those of formed through the cold crystallization from the glassy state (Ling & Spruiell, 2006). The known slow crystallization ability of PLA and quick cooling process caused that quenched samples remained mostly amorphous that was proved by low value of specific enthalpy ΣΔH. Thermograms of annealed samples displayed a marked double melting peak showing high degree of crystallinity (Gregorova et al.; 2011b). The presence of filler marginally decreased specific enthalpy values of PLA. The presence of multiple melting peaks in thermograms of annealed samples can be explained by applied annealing that induce other crystal population, namely α´ (initially created with grain like morphology) and α (during annealing created with spherulitic morphology) crystals (Zhang et al., 2008; Pan et al., 2008). Melting temperature for unannealed neat or filled PLA samples were recorded between 134-140°C for the first melting peak and 150-151°C for the second melting peak. The growth of crystals during annealing increased the values of temperature of both melting peaks depending on the mixture composition. The change in the value of the ratio of the first and the second melting peaks indicates the modification of size of the present crystals. The Tg value after an annealing treatment can be taken as an indicator for the occurred changes in an amorphous/crystal ratio but also in PLA/filler interaction level. The increase of an interfacial compatibility between wood filler and poly(lactic acid) can be detected by an shift of a glass transition to the higher temperature (Gregorova & Wimmer, 2012).


6. Conclusions

Differential scanning calorimetry is the method to characterize thermal behavior of polymeric materials on the base of the differences obtained in the heat flow between a sample and a reference under various temperature programs. In the addition to the quality and compositional analyses of polymers, DSC is applicable to the investigation of the thermal changes occurring in polymer systems during chemical reactions (e.g. polymerisation), oxidative degradation, vaporization, sublimation and desorptionThe selection of a proper temperature program is an important issue for the proper DSC analysis (e.g. a position and a shape of melting peak depend inherently on the nature of polymer and on the used heating scan rate). Thermal properties of biopolymers depend on many factors such as their natural origin, purity, composition, processing, thermal treatment, mechanical stressing, and aging. In this chapter, non-isothermal DSC was introduced as an method to investigate thermal properties of biopolymers, namely amorphous lignin and semi-crystalline poly(lactic acid). It can be concluded that DSC is one of the available methods to determine thermal properties of lignin with various molecular weight properties and composition.. Moreover, DSC can serve as a method to determine stabilizing effect of lignin used as an additive in polymer samples. Furthermore, DSC can be used as the quick method to measure melting behavior and the crystallinity of poly(lactic acid). The thermal history during polymer processing as well as the incorporation of some filler (e.g. wood flour) or additives can modify the crystallinity of PLA. The percentage of the crystallinity is one of the most important characteristics that influence its physico-mechanical behavior (stiffness, toughness, brittleness, barrier resistance, thermal stability and optical clarity). DSC is the valuable method for the investigation of thermal properties of biopolymers. However, it is necessary to use also the other additional physical and chemical testing methods to obtain complex data describing biopolymers, such as lignin and poly(lactic acid).


  1. 1. BowerB. I2002An Introduction to Polymer Physics. Cambridge University Press, New York
  2. 2. Di LorenzoM.L. (2006The Crystallization and Melting Processes of Poly(L-lactic acid). Macromol. Symp., 234176183
  3. 3. GregorovaACibulkovaZKosikovaBSimonP2005aStabilization effect of lignin in polypropylene and recycled polypropylene. Polymer Degradation and Stability, 8935535580141-3910
  4. 4. GregorovaAKosikovaBOsvaldA2005bThe study of lignin influence on properties of polypropylene composites. Wood Research, 50241481336-4561
  5. 5. GregorovaASchalliMStelzerF2011aFunctionalization of polylactic acid through azeotropic dehydrative condensation. 19th Annual Meeting of the BioEnvironmental Polymer Society BEPS, Book of Abstracts, PO-4, 978-3-95029-923-6Vienna Austria, September 2011
  6. 6. GregorovaASedlarikVPastorekMJachandraHStelzerF2011bEffect of compatibilizing agent on the properties of highly crystalline composites based on poly(lactic acid) and wood flour and/or mica. Journal of Polymers and the Environment, 1923723811566-2543
  7. 7. GregorovaA& Wimmer R. (2012Filler-Matrix Compatibility of Poly(lactic acid) Based Composites. In: Piemonte V., Editor. Polylactic Acid: Synthesis, Properties and Applications, Piemonte, V., 97119Nova Science Publishers NY, 978-1-62100-348-9
  8. 8. HatakeyamaHHatakeyamaT2010Lignin Structure, Properties and Applications. In: Biopolymers Lignin, Proteins, Bioactive Nanocomposites, Abe A., Dusek K., Kobayashi S., 1112Springer-Verlag Berlin Heidelberg
  9. 9. HrabalovaMGregorovaAWimmerRSedlarikVMachovskyMMundiglerN2010Effect of Wood Flour Loading and Thermal Annealing on Viscoelastic Properties of Poly(lactic acid) Composite Films. Journal of Applied Polymer Science, 1183153415401097-4628
  10. 10. KopinkeF. DRemmlerMMackenzieKMöderMWachsenO1996Thermal decomposition of biodegradable polyesters-II. Poly(lactic acid). Polymer Degradation and Stabilility, 5333293420141-3910
  11. 11. KosikovaBKacurakovaMDemianovaV1993aPhotooxidation of the composite lignin/polypropylene films. Chemical Papers, 471321360366-6352
  12. 12. KosikovaBDemianovaVKacurakovaM1993bSulphur-freelignins as composites of polypropylene films. Journal of Applied Polymer Science, 476106510731097-4628
  13. 13. KosikovaBGregorovaAOsvaldAKrajcovicovaJ2007Role of Lignin Filler in Stabilization of Natural Rubber-Based Composites. Journal of Applied Polymer Science, 1032122612311097-4628
  14. 14. LeeS-HKimS. HHanY-KKimY. H2001Synthesis and degradation of end-group-functionalized polylactide. Journal of Polymer Science Part A: Polymer Chemistry, 3979739851099-0518
  15. 15. LingXSpruiellJ. E2006Analysis of the complex thermal behaviour of poly(L-lactic acid) film. I. Samples crystallized from the glassy state. Journal of Polymer Science Part B: Polymer Physics, 4422320032141099-0488
  16. 16. MathewA. POksmanKSainM2005Mechanical properties of biodegradable composites from poly lactic acid (PLA) and microcrystalline cellulose (MCC). Journal of Applied Polymer Science, 975201420151097-4628
  17. 17. MathewA. POksmanKSainM2006The effect of morphology and chemical characteristics of cellulose reinforcements on the crystallinity of polylactic acid. Journal of Applied Polymer Science, 10113003101097-4628
  18. 18. MikulasovaMKosikovaB1999Biodegradability of lignin-polypropylene composite films. Folia Microbiologica, 446696720015-5632
  19. 19. PanPZhuBKaiWDongTInoueY2008Polymorphic Transition in Disordered Poly(L-lactide) Crystals Induced by Annealing at Elevated Temperatures. Macromolecules, 4112429643041520-5835
  20. 20. PillaSGongSONeilERowellMKrzysikA. M2008Polylactide-pinewood flour composites. Polymer Engineering Science, 4835785871548-2634
  21. 21. RamkumarD. H. SBhattacharyaM1998Steady shear and dynamic properties of biodegradable polyesters. Polymer Engineering Science, 389142614351548-2634
  22. 22. YasuniwaMTsubakiharaSSugimotoYNakafukuC2004Thermal analysis of the double-melting behavior of poly(L-Lactic acid). Journal of Polymer Science Part B: Polymer Physics, 42125321099-0488
  23. 23. YasuniwaMTsubakiharaSIuraKOnoYDanYTakashashiK2006Crystallization behavior of Poly(L-lactic acid). Polymer, 4721755475630032-3861
  24. 24. YasuniwaMIuraKDanY2007Melting behavior of poly(L-lactic acid): Effects of crystallization temperature and time. Polymer, 4818539854070032-3861
  25. 25. ZhangJTashiroKTsujiHDombA. J2008Disorder-to-Order Phase Transition and Multiple Melting Behavior of Poly(L-lactide) Investigated by Simultaneous Measurements of WAXD and DSC. Macromolecules, 414135213571520-5835

Written By

Adriana Gregorova

Submitted: 21 November 2011 Published: 23 January 2013