More than half of the publishers listed alongside IntechOpen (18 out of 30) are Social Science and Humanities publishers. IntechOpen is an exception to this as a leader in not only Open Access content but Open Access content across all scientific disciplines, including Physical Sciences, Engineering and Technology, Health Sciences, Life Science, and Social Sciences and Humanities.
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Our breakdown of titles published demonstrates this with 47% PET, 31% HS, 18% LS, and 4% SSH books published.
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“Even though ItechOpen has shown the potential of sci-tech books using an OA approach,” other publishers “have shown little interest in OA books.”
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Additionally, each book published by IntechOpen contains original content and research findings.
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We are honored to be among such prestigious publishers and we hope to continue to spearhead that growth in our quest to promote Open Access as a true pioneer in OA book publishing.
Simba Information has released its Open Access Book Publishing 2020 - 2024 report and has again identified IntechOpen as the world’s largest Open Access book publisher by title count.
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Simba Information is a leading provider for market intelligence and forecasts in the media and publishing industry. The report, published every year, provides an overview and financial outlook for the global professional e-book publishing market.
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IntechOpen, De Gruyter, and Frontiers are the largest OA book publishers by title count, with IntechOpen coming in at first place with 5,101 OA books published, a good 1,782 titles ahead of the nearest competitor.
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Since the first Open Access Book Publishing report published in 2016, IntechOpen has held the top stop each year.
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More than half of the publishers listed alongside IntechOpen (18 out of 30) are Social Science and Humanities publishers. IntechOpen is an exception to this as a leader in not only Open Access content but Open Access content across all scientific disciplines, including Physical Sciences, Engineering and Technology, Health Sciences, Life Science, and Social Sciences and Humanities.
\n\n
Our breakdown of titles published demonstrates this with 47% PET, 31% HS, 18% LS, and 4% SSH books published.
\n\n
“Even though ItechOpen has shown the potential of sci-tech books using an OA approach,” other publishers “have shown little interest in OA books.”
\n\n
Additionally, each book published by IntechOpen contains original content and research findings.
\n\n
We are honored to be among such prestigious publishers and we hope to continue to spearhead that growth in our quest to promote Open Access as a true pioneer in OA book publishing.
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\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"5347",leadTitle:null,fullTitle:"Nanostructured Solar Cells",title:"Nanostructured Solar Cells",subtitle:null,reviewType:"peer-reviewed",abstract:"Nanostructured solar cells are very important in renewable energy sector as well as in environmental aspects, because it is environment friendly. The nano-grating structures (such as triangular or conical shaped) have a gradual change in refractive index which acts as a multilayer antireflective coating that is leading to reduced light reflection losses over broadband ranges of wavelength and angle of incidence. There are different types of losses in solar cells that always reduce the conversion efficiency, but the light reflection loss is the most important factor that decreases the conversion efficiency of solar cells significantly. The antireflective coating is an optical coating which is applied to the surface of lenses or any optical devices to reduce the light reflection losses. This coating assists for the light trapping capturing capacity or improves the efficiency of optical devices, such as lenses or solar cells. Hence, the multilayer antireflective coatings can reduce the light reflection losses and increases the conversion efficiency of nanostructured solar cells.",isbn:"978-953-51-2936-3",printIsbn:"978-953-51-2935-6",pdfIsbn:"978-953-51-4110-5",doi:"10.5772/62516",price:139,priceEur:155,priceUsd:179,slug:"nanostructured-solar-cells",numberOfPages:314,isOpenForSubmission:!1,isInWos:1,isInBkci:!0,hash:"1856c1630d95d64906a0f1a3e597cb0a",bookSignature:"Narottam Das",publishedDate:"February 22nd 2017",coverURL:"https://cdn.intechopen.com/books/images_new/5347.jpg",numberOfDownloads:30225,numberOfWosCitations:60,numberOfCrossrefCitations:31,numberOfCrossrefCitationsByBook:1,numberOfDimensionsCitations:58,numberOfDimensionsCitationsByBook:1,hasAltmetrics:1,numberOfTotalCitations:149,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"February 17th 2016",dateEndSecondStepPublish:"March 9th 2016",dateEndThirdStepPublish:"June 13th 2016",dateEndFourthStepPublish:"September 11th 2016",dateEndFifthStepPublish:"October 11th 2016",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,7,8",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"15357",title:"Dr.",name:"Narottam",middleName:null,surname:"Das",slug:"narottam-das",fullName:"Narottam Das",profilePictureURL:"https://mts.intechopen.com/storage/users/15357/images/3100_n.jpg",biography:"Narottam Das received BSc and MSc degree in Electrical and Electronic Engineering from Chittagong University of Engineering & Technology, and Bangladesh University of Engineering & Technology, Bangladesh, respectively. He received PhD degree in Systems and Information Engineering from Yamagata University, Japan in 2000. Currently he is working as a Lecturer at the School of Mechanical and Electrical Engineering, University of Southern Queensland, Toowoomba, QLD, Australia. He is also an Adjunct Senior Research Fellow at the Department of Electrical and Computer Engineering, Curtin University, Perth, Australia. Earlier, he worked at Curtin University, Edith Cowan University and Monash University, Australia; NEC Yamagata Ltd., Japan and Bangladesh Export Import Company, Dhaka. He is the author/co-author of books, book chapters, over 135 referred international journal papers and conference proceedings, and over 20 industrial technical reports at NEC Japan. Dr. Das is a senior member of the IEEE, member of the Institute of Engineers, Australia, and life fellow of the Institute of Engineers, Bangladesh.",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"4",totalChapterViews:"0",totalEditedBooks:"4",institution:{name:"Central Queensland University",institutionURL:null,country:{name:"Australia"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"770",title:"Renewable Energy",slug:"engineering-energy-engineering-renewable-energy"}],chapters:[{id:"54030",title:"Introduction of Nano-Structured Solar Cells",doi:"10.5772/67467",slug:"introduction-of-nano-structured-solar-cells",totalDownloads:1691,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:null,signatures:"Narottam Das",downloadPdfUrl:"/chapter/pdf-download/54030",previewPdfUrl:"/chapter/pdf-preview/54030",authors:[{id:"15357",title:"Dr.",name:"Narottam",surname:"Das",slug:"narottam-das",fullName:"Narottam Das"}],corrections:null},{id:"52535",title:"Third-Generation-Sensitized Solar Cells",doi:"10.5772/65290",slug:"third-generation-sensitized-solar-cells",totalDownloads:2505,totalCrossrefCites:6,totalDimensionsCites:11,hasAltmetrics:0,abstract:"The need to produce renewable energy with low production cost is indispensable in making the dream of avoiding undue reliance on non-renewable energy a reality. The emergence of a third-generation photovoltaic technology that is still in the infant stage gives hope for such a dream. Solar cells sensitized by dyes, quantum dots and perovskites are considered to be third-generation technological devices. This research focuses on the development of suitable and reliable sensitizers to widen electromagnetic (EM) wave absorption and to ensure stability of the photovoltaic system. This article discusses the basic principles and the progress in sensitized photovoltaics.",signatures:"Muhammad Ammar Mingsukang, Mohd Hamdi Buraidah and\nAbdul Kariem Arof",downloadPdfUrl:"/chapter/pdf-download/52535",previewPdfUrl:"/chapter/pdf-preview/52535",authors:[{id:"186084",title:"Dr.",name:"Abdul Kariem",surname:"Arof",slug:"abdul-kariem-arof",fullName:"Abdul Kariem Arof"}],corrections:null},{id:"53636",title:"Optoelectronics and Bio Devices on Paper Powered by Solar Cells",doi:"10.5772/66695",slug:"optoelectronics-and-bio-devices-on-paper-powered-by-solar-cells",totalDownloads:1958,totalCrossrefCites:4,totalDimensionsCites:8,hasAltmetrics:1,abstract:"The employment of printing techniques as cost-effective methods to fabricate low cost, flexible, disposable and sustainable solar cells is intimately dependent on the substrate properties and the adequate electronic devices to be powered by them. Among such devices, there is currently a growing interest in the development of user-oriented and multipurpose systems for intelligent packaging or on-site medical diagnostics, which would greatly benefit from printable solar cells as their energy source for autonomous operation.",signatures:"António T. Vicente, Andreia Araújo, Diana Gaspar, Lídia Santos,\nAna C. Marques, Manuel J. Mendes, Luís Pereira, Elvira Fortunato\nand Rodrigo Martins",downloadPdfUrl:"/chapter/pdf-download/53636",previewPdfUrl:"/chapter/pdf-preview/53636",authors:[{id:"186314",title:"M.Sc.",name:"António",surname:"Vicente",slug:"antonio-vicente",fullName:"António Vicente"},{id:"197377",title:"Prof.",name:"Rodrigo",surname:"Martins",slug:"rodrigo-martins",fullName:"Rodrigo Martins"}],corrections:null},{id:"52161",title:"Silicon Heterojunction Solar Cells: The Key Role of Heterointerfaces and their Impact on the Performance",doi:"10.5772/65020",slug:"silicon-heterojunction-solar-cells-the-key-role-of-heterointerfaces-and-their-impact-on-the-performa",totalDownloads:3226,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"This chapter is dedicated to the processes linked with the collection of photo-generated carriers in silicon heterojunction (SHJ) solar cells with a focus on the key role of the amorphous silicon/crystalline silicon heterojunction. The intention is to explain the role of carrier inversion at the heterointerface and connect it with the properties of the SHJ to obtain deeper understanding of carrier transport properties and collection, which goes beyond amorphous silicon-based structures and will contribute to understanding the new emerging SHJ based on amorphous silicon oxide and metal oxide emitter layers. The study is extended by a simulation of the TCO/emitter interface with the aim to reveal the effect of parasitic Schottky barrier height on the performance of the SHJ solar cell. In addition, the simulation study of SHJ under concentrated light and varied temperatures is outlined to show the main limitations and prospects of SHJ structures for utilization under concentrated light.",signatures:"Miroslav Mikolášek",downloadPdfUrl:"/chapter/pdf-download/52161",previewPdfUrl:"/chapter/pdf-preview/52161",authors:[{id:"41342",title:"Mr",name:"Miroslav",surname:"Mikolasek",slug:"miroslav-mikolasek",fullName:"Miroslav Mikolasek"}],corrections:null},{id:"52139",title:"High‐Efficiency Front Junction n‐Type Crystalline Silicon Solar Cells",doi:"10.5772/65023",slug:"high-efficiency-front-junction-n-type-crystalline-silicon-solar-cells",totalDownloads:3021,totalCrossrefCites:3,totalDimensionsCites:5,hasAltmetrics:0,abstract:"This chapter aims to provide students/workers in the field of photovoltaics with the valuable information and knowledge needed to understand the physics and operation of high‐efficiency front junction n‐type crystalline silicon solar cells. The surface recombination and passivation mechanisms, and several promising passivation schemes for front and back cell surfaces, are addressed and reviewed. The advanced cell structures and their fabrication schemes to achieve higher efficiency are described and discussed, including selective emitter on the front and locally doped back surface filed or carrier selective rear contact composed of tunnel oxide and phosphorus‐doped polycrystalline silicon thin film. These advanced cell design features have become highly active areas of investigations in the photovoltaic industry for next‐generation production cells.",signatures:"Yuguo Tao and Ajeet Rohatgi",downloadPdfUrl:"/chapter/pdf-download/52139",previewPdfUrl:"/chapter/pdf-preview/52139",authors:[{id:"181116",title:"Dr.",name:"Yuguo",surname:"Tao",slug:"yuguo-tao",fullName:"Yuguo Tao"},{id:"186311",title:"Prof.",name:"Ajeet",surname:"Rohatgi",slug:"ajeet-rohatgi",fullName:"Ajeet Rohatgi"}],corrections:null},{id:"52129",title:"Ultrafast Time‐Resolved Measurements of Hybrid Solar Cells",doi:"10.5772/65022",slug:"ultrafast-time-resolved-measurements-of-hybrid-solar-cells",totalDownloads:1514,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The early time charge carrier dynamics in quantum dot‐sensitized and organo‐metal halide perovskite solar cells are presented in this chapter. Using transient spectroscopy techniques, i.e., absorption, photoluminescence, and photoconductivity, we probed the generation mechanism, charge injection, mobility, and recombination of charges in the time scales of subpicosecond (ps) to a nanosecond. In few ps, electron injection from quantum dot to n‐type metal oxide (MO) is complete while hole injection to p‐type MO required hundreds of ps. The injection process is dictated by the band alignment, density of states of MO and the charge transfer state at the interface. For organo‐metal halide perovskite material, there is a distribution of exciton binding energy brought about by the nonuniformity in the quality of the sample. As a result, varying amount of exciton and highly mobile charges may be generated depending on the morphology of the film. In the sample presented here, we found that 30% of photo‐generated charges are excitons, which then dissociates within 2–3 ps. The rest of the photons are instantaneously converted into highly mobile charges (µe = 12.5 cm2 V-1 s-1 and µh = 7.5 cm2 V-1 s-1), and at the appropriate excitation fluence, the photoconductivity remains constant up to 1 ns. The time scale and mechanism of charge injection from perovskite into organic electrodes are also presented.",signatures:"Kaibo Zheng and Carlito S. Ponseca",downloadPdfUrl:"/chapter/pdf-download/52129",previewPdfUrl:"/chapter/pdf-preview/52129",authors:[{id:"175823",title:"Dr.",name:"Carlito Jr.",surname:"Ponseca",slug:"carlito-jr.-ponseca",fullName:"Carlito Jr. Ponseca"},{id:"193840",title:"Dr.",name:"Kaibo",surname:"Zheng",slug:"kaibo-zheng",fullName:"Kaibo Zheng"}],corrections:null},{id:"52513",title:"Plasmonic Thin Film Solar Cells",doi:"10.5772/65388",slug:"plasmonic-thin-film-solar-cells",totalDownloads:2787,totalCrossrefCites:4,totalDimensionsCites:6,hasAltmetrics:0,abstract:"Thin film solar cell technology represents an alternative way to effectively solve the world’s increasing energy shortage problem. Light trapping is of critical importance. Surface plasmons (SPs), including both localized surface plasmons (LSPs) excited in the metallic nanoparticles and surface plasmon polaritons (SPPs) propagating at the metal/semiconductor interfaces, have been so far extensively investigated with great interests in designing thin film solar cells. In this chapter, plasmonic structures to improve the performance of thin film solar cell are reviewed according to their positions of the nanostructures, which can be divided into at least three ways: directly on top of thin film solar cell, embedded at the bottom or middle of the optical absorber layer, and hybrid of metallic nanostructures with nanowire of optical absorber layer.",signatures:"Qiuping Huang, Xiang Hu, Zhengping Fu and Yalin Lu",downloadPdfUrl:"/chapter/pdf-download/52513",previewPdfUrl:"/chapter/pdf-preview/52513",authors:[{id:"185736",title:"Prof.",name:"Yalin",surname:"Lu",slug:"yalin-lu",fullName:"Yalin Lu"},{id:"186251",title:"Prof.",name:"Zhengping",surname:"Fu",slug:"zhengping-fu",fullName:"Zhengping Fu"},{id:"186252",title:"Dr.",name:"Qiuping",surname:"Huang",slug:"qiuping-huang",fullName:"Qiuping Huang"},{id:"186253",title:"Dr.",name:"Xiang",surname:"Hu",slug:"xiang-hu",fullName:"Xiang Hu"}],corrections:null},{id:"52630",title:"Interface Engineering and Electrode Engineering for Organic Solar Cells",doi:"10.5772/65312",slug:"interface-engineering-and-electrode-engineering-for-organic-solar-cells",totalDownloads:1754,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Interface engineering and electrode engineering play important roles in the performance improvement for organic solar cells (OSCs). We here would investigate the effect of various cathode modifying layers and ITO-free electrodes on the device performance. First, for inverted organic solar cells (IOSCs) with a poly (3-hexylthiophene-2,5-diyl):[6,6]-phenyl C61 butyric acid methyl ester blend, an aqueous solution method using low temperatures is adopted to deposit a ZnO interlayer in IOSCs. When the ZnO annealing temperature is above 80°C, the corresponding IOSCs show senior PCEs over 3.5%. Meanwhile the flexible devices based on poly(ethylene terephthalate) substrate display a PCE of 3.26% and good flexibility. Second, the performance of IOSCs based on AZO cathode and Ca modifier are studied. The resulted IOSCs with an ultrathin Ca modifier (~1 nm) could achieve a senior PCE above 3%, and highly efficient electron transport at AZO/Ca/organic interface, which obviously weakens the light soaking issue. Third, by introducing a 2 nm MoO3 interlayer for Ag anode deposition, the obtained OSCs show an improved PCE of 2.71%, and the flexible device also achieves a comparable PCE of 2.50%. All these investigations may be instructive for further improvement of device performance and the possible commercialization in the future.",signatures:"Dazheng Chen and Chunfu Zhang",downloadPdfUrl:"/chapter/pdf-download/52630",previewPdfUrl:"/chapter/pdf-preview/52630",authors:[{id:"185737",title:"Dr.",name:"Dazheng",surname:"Chen",slug:"dazheng-chen",fullName:"Dazheng Chen"},{id:"193641",title:"Prof.",name:"Chunfu",surname:"Zhang",slug:"chunfu-zhang",fullName:"Chunfu Zhang"}],corrections:null},{id:"52325",title:"Copper Indium Gallium Selenide Thin Film Solar Cells",doi:"10.5772/65291",slug:"copper-indium-gallium-selenide-thin-film-solar-cells",totalDownloads:2719,totalCrossrefCites:2,totalDimensionsCites:3,hasAltmetrics:0,abstract:'The solar energy as one of the new energy sources and a regenerated energy is abundant and pollution-free. Most photovoltaic devices (solar cells) sold in the market today are based on silicon wafers, the so-called "first generation" technology. The market at present is on the verge of switching to a "second generation" of thin film solar cell technology which offers prospects for a large reduction in material costs by eliminating the costs of the silicon wafers. Cadmium telluride (CdTe), amorphous silicon (a-Si) and copper indium gallium selenide (CIGS) are three thin film technologies which have achieved commercial production. This chapter gives the review of the CIGS solar cells regarding the heterostructures, materials, technology and research advances. It also states the key findings in our research and provides suggestions for future research.',signatures:"Yang Tang",downloadPdfUrl:"/chapter/pdf-download/52325",previewPdfUrl:"/chapter/pdf-preview/52325",authors:[{id:"185929",title:"Dr.",name:"Yang",surname:"Tang",slug:"yang-tang",fullName:"Yang Tang"}],corrections:null},{id:"52235",title:"ZnO-Based Electron Transporting Layer for Perovskite Solar Cells",doi:"10.5772/65056",slug:"zno-based-electron-transporting-layer-for-perovskite-solar-cells",totalDownloads:3155,totalCrossrefCites:8,totalDimensionsCites:14,hasAltmetrics:1,abstract:"Recently, organic/inorganic hybrid perovskite materials, APbX3 (A = CH3NH3 or HC(NH2)2; X = I, Br or Cl), have attracted much interest for their promising application in solar cells as the light-absorbing component to their broad spectral absorption, strong light-harvesting and long exciton diffusion length. The perovskite solar cells (PSCs) can reduce the production costs and achieve high power conversion efficiency significantly compared to standard silicon cells and other thin film cells. On the other hand, ZnO based materials have been recently investigated in the PSCs devices as electron injection layers for low-temperature, low-cost and flexible devices. This chapter aims to review PSCs using ZnO materials as electron extraction layers. We will discuss the electron transmission and effect of the electron-transporting layer in PSCs and the preparation method of the ZnO. ZnO is a potential material for many applications due to their high electron mobility, transparent and various nanostructure. The ZnO was introduced into the PSCs structure to improve electron extraction efficiency. This chapter summaries the effect and parameters of PSCs based on the ZnO layer/nanostructure prepared by several methods as electron transport layers.",signatures:"Lung-Chien Chen and Zong-Liang Tseng",downloadPdfUrl:"/chapter/pdf-download/52235",previewPdfUrl:"/chapter/pdf-preview/52235",authors:[{id:"151925",title:"Prof.",name:"Lung-Chien",surname:"Chen",slug:"lung-chien-chen",fullName:"Lung-Chien Chen"}],corrections:null},{id:"52651",title:"Fabrication and Characterization of Element-Doped Perovskite Solar Cells",doi:"10.5772/65768",slug:"fabrication-and-characterization-of-element-doped-perovskite-solar-cells",totalDownloads:1697,totalCrossrefCites:1,totalDimensionsCites:3,hasAltmetrics:0,abstract:"Perovskite solar cells were fabricated and characterized. X-ray diffraction analysis and transmission electron microscopy were used for investigation of the devices. The structure analysis by them showed structural transformation of the crystal structure of the perovskite, which indicated that a cubic-tetragonal crystal system depended on the annealing condition. The photovoltaic properties of the cells also depended on the structures. Metal doping and halogen doping to the perovskite and TiO2 were also investigated. The results showed an increase in the efficiencies of the devices, due to the structural change of the perovskite compound layers.",signatures:"Takeo Oku, Masahito Zushi, Kohei Suzuki, Yuya Ohishi, Taisuke\nMatsumoto and Atsushi Suzuki",downloadPdfUrl:"/chapter/pdf-download/52651",previewPdfUrl:"/chapter/pdf-preview/52651",authors:[{id:"25854",title:"Dr.",name:"Atsushi",surname:"Suzuki",slug:"atsushi-suzuki",fullName:"Atsushi Suzuki"},{id:"31132",title:"Prof.",name:"Takeo",surname:"Oku",slug:"takeo-oku",fullName:"Takeo Oku"},{id:"194955",title:"Mr.",name:"Masahito",surname:"Zushi",slug:"masahito-zushi",fullName:"Masahito Zushi"},{id:"194956",title:"Mr.",name:"Kohei",surname:"Suzuki",slug:"kohei-suzuki",fullName:"Kohei Suzuki"},{id:"194957",title:"MSc.",name:"Taisuke",surname:"Matsumoto",slug:"taisuke-matsumoto",fullName:"Taisuke Matsumoto"},{id:"194958",title:"Mr.",name:"Yuya",surname:"Ohishi",slug:"yuya-ohishi",fullName:"Yuya Ohishi"}],corrections:null},{id:"52879",title:"Perovskite as Light Harvester: Prospects, Efficiency, Pitfalls and Roadmap",doi:"10.5772/65052",slug:"perovskite-as-light-harvester-prospects-efficiency-pitfalls-and-roadmap",totalDownloads:1676,totalCrossrefCites:1,totalDimensionsCites:2,hasAltmetrics:0,abstract:"In the recent years, perovskite materials have attracted great attention due to their excellent light‐harvesting properties. The organic materials of these hybrid inorganic organic light harvesters are used as sensitizers and the inorganic materials have been used as light absorbers. The exceptional properties of these materials such as long diffusion length, high carrier mobility, affordable device fabrication, and adjustable adsorption range have created a new era in optoelectronic technologies. The perovskites have become promising materials due of their versatility in device architecture, flexibility in material growth, and ability to achieve the high efficiency through various processing techniques. The superior performance of silicon‐based tandems by achieving efficiency more than 40% has encouraged researchers to further expand the investigations to higher levels. The quest to transit the research curiosity to the market photovoltaic technology has given a new dimension to the remarkable ascension of perovskite solar cells. This chapter introduces the experimental and theoretical aspects, the electrical and optical properties, pitfalls, and a roadmap for the future prospects of perovskite materials.",signatures:"Ruby Srivastava",downloadPdfUrl:"/chapter/pdf-download/52879",previewPdfUrl:"/chapter/pdf-preview/52879",authors:[{id:"185788",title:"Dr.",name:"Ruby",surname:"Srivastava",slug:"ruby-srivastava",fullName:"Ruby Srivastava"}],corrections:null},{id:"52374",title:"Recent Progresses in Perovskite Solar Cells",doi:"10.5772/65019",slug:"recent-progresses-in-perovskite-solar-cells",totalDownloads:2530,totalCrossrefCites:2,totalDimensionsCites:4,hasAltmetrics:0,abstract:"Perovskite solar cell (PSC) can be regarded as a continuation of dye sensitized solar cell (DSSC) in terms of the sensitization phenomena that occurred in the functioning molecules. In 2012, a breakthrough propose has been made for the sensitization of PSCs, in which a solid‐state structure is offered as an equivalent sensitizer used in DSSC. The power conversion efficiency (PCE) of those solid‐state cells reached about twofold of its initial value during the past several years. Immediately after, the researchers followed this propose worldwide. They have introduced an improved efficiency of as much as 20%, which was originally started from its initial value of 4%, just in 4 years. Thus, the new concept, solid perovskite molecules, has eliminated the need for the liquid electrolyte in DSSC while still carrying the advantages of organic solar cells (OSCs). Therefore, the distinctive material of PSC—the organometallic halide molecules (also known as OMH or organic‐inorganic trihalides)—inclined an unexpected reputation for solar cell (SC) researches. Hence, it seems that we will witness a new age for solar conversion devices depending on the recent hopeful progresses on PSCs. The high rate of photovoltaic (PV) conversion capacity in PSC is generally expressed by the basic properties possessed by the organic‐inorganic perovskite crystal, such as better optical properties and well diffused charges along huge distances during the charge transport. In addition, a low temperature processing is applicable during its production. Moreover, the perovskite layer provides a tunable band gap. Therefore, depending on better developments on designed molecules, PSC may gain extreme performances compared to the other competitors, such as OSC or DSSC devices. This chapter starts with a general discussion on the need for an affordable clean energy conversion device that is urgent for the future of humanity, due to publicly well‐known global warming issue. In Section 2, basic properties of PSC are mentioned together with their structure and working principles. Section 3 continues with an overview on organometallic perovskite molecules after a brief introductory history is presented. The absorption and band gap properties are also discussed. Since most perovskite materials need a hole transporting material (HTMs) within the PSC, the kinds of HTMs that are designed for PSCs are described in Section 3. The rendering of long‐term stabilization has special importance for PSCs since the instability issue remained idle in spite of those recent increased efficiency values attained by various research groups. Therefore, the stability issues are discussed in a separate part in Section 4. We finally close the chapter discussing the challenges and opportunities relying on the chapter content. We note that the recent investigations on PSCs have special importance for its large‐scale realization in order to make them ready for the photovoltaic industry of the future. Hence, there are various announced meetings focusing on its mass production due to the unexpected sharp rise of the perovskite efficiency in the last 6 years. 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1. Introduction
Nowadays, to satisfy the increase of internet demands and requirement, two multiplexing techniques are used: WDM (Wavelength Division Multiplex) and TDM (Time Division Multiplex). WDM still more used than TDM. However, for practical system applications, such as optical CDMA (Code Division Multiplex Access) and OTDM (Optical Time Division Multiplex) systems, high speed optical communications require light sources with a repetition rate control. In this area, pulsed fiber lasers have become very attractive.
Fiber lasers have a number of qualities which make them very attractive for ultra short pulses generation via Q-switching, active or passive mode locking mechanisms. The gain bandwidth of rare-earth-doped fibers is large, typically tens of nanometers, which allows the generation of femtosecond pulses. The high gain efficiency of active fibers makes possible such lasers to operate with fairly low pump powers and tolerate intra cavity optical elements with relatively high optical losses. Fiber laser setups are very compact and can be done with a low cost. Furthermore, mode locked fiber lasers can rely on telecom components.
2. Q-switching mechanism
Storing ions in a higher energy level can be achieved by limiting ions flow to the bottom level. So, it’s necessary to prevent stimulated emission prevalence.
By means of light modulators able to generate high optical powers when transiting between the off and on states, we prevent light propagate within the laser cavity. For a radiative transition, the only possible drain to the bottom level is caused by spontaneous emission (see Fig. 1). The E2 level population very significant, the cavity losses are suddenly reduced and the oscillation becomes possible. The stimulated emission becomes prevalent and the laser starts emitting abruptly. All ions stored up go down emitting stimulated photons (see Fig. 2).
Figure 1.
Q-switching first step.
Figure 2.
Q-switching second step.
At a given time, there is no way for stimulated emission to happen and the cavity is emptied by resulting losses of the output mirror (see Fig. 3).
The abrupt variation of the number of photons into the cavity results in emitting a high peak power optical pulse. Generally, several journeys between the two mirrors are necessary to completely depopulate the up-level and empty the cavity. So, the pulse width would be higher than the time of a coming and going through the cavity. With lengths lower than one meter, it is possible to generate nanosecond pulses. The repetition rate varies between few hundreds of Hz and few hundreds of KHz.
The Q quality factor of a laser cavity describes its capacity to store the energy light in standing waves. The factor Q is the ratio between the stored and the lost energies after each round trip through the cavity. In fact a Q-switch device is an optical modulatorable to control the energy losses of the cavity with generally a repetition rate varying between 1 and 100 KHz [1].
Figure 3.
Q-switching third step.
3. Mode locking mechanism
In a laser cavity, frequencies circulating into the resonator and having more gain than losses are called longitudinal modes. They can be considered as an assembly of independent oscillators. These modes gain increases after each round trip through the cavity. These modes are separated by ΔF = 1/TF = v/2L for a linear cavity case of Fabry Perrot cavity or v/L for a loop cavity case of fiber laser.L is the cavity length and v is the light speed. When these modes oscillate independently of each other, the laser emits continuously. Fig. 4 illustrates a laser cavity output signal resulting on the propagation of three independent longitudinal modes. However, when a fixed phase shift exists between the various modes, the cavity emits a pulses train and becomes phase locked. Fig. 5 shows a mode locked laser cavity output signal resulting on the propagation of three phase dependent longitudinal modes. In fact,the mode locking technique consists in creating a certain phase relationship between the different modes oscillating into the cavity.
Figure 4.
Output signal from laser operating without mode locking mechanism.
Figure 5.
Mode locked laser output signal.
If we consider M=2S+1 optical modes with S an integer and Aqthe complex envelope of mode q, the complex wave of the q mode and the total signal propagating into the cavity are respectively:
Fig. 6 shows the resulting output pulses sequence of a mode locked laser cavity allowing the oscillation of M longitudinal modes. The mode locking mechanism allows having pulses train with peak power M-times more significant than the average power.
Figure 6.
Mode locked laser output I(t,z) [2].
4. Pulsed fiber laser
In case of fiber laser, the 100% reflective mirror is replaced by the optical fiber loop, the output mirror by an output coupler and the active laser medium by an optical amplifier such as Erbium Doped Fiber Amplifier. Many sophisticated resonator setups have been used particularly for mode-locked fiber lasers, generating picosecond or femtosecond pulses. A fiber laser can contain an electro-optic modulator, an acousto-optic modulator or a saturable absorber to actively or passively mode lock the different longitudinal modes oscillating in the cavity.
Figure 7.
Different types of pulsed fiber lasers.
Passively mode locked fiber lasers have the advantage of being entirely consisted of optical components. They do not require external electrical components and the mode locking mechanism in the cavity is carried out automatically [3-4-5]. However, these lasers can’t reach high pulses repetition rates. In fact, the repetition rate of generated pulses depends mainly on the cavity length [6-7]. The laser resonator may contain a saturable absorber such as SESAM (Semiconductor Saturable Absorber Mirror) to passively mode lock the cavity (see Fig. 8).
The effect of NLPR(Non Linear Polarization Rotation), as illustrated in Fig. 9, or a nonlinear fiber loop mirror, as illustrated in Fig. 10, can be used as artificial saturable absorbers [8].
Figure 9.
NLPR mechanism.
A nonlinear loop mirror is used in a “figure-of-eight laser”. A schematic diagram of the 8FL (Eight Fiber Laser) is shown in Fig. 10. The 8FL overall design is that of a ring cavity with a Sagnac interferometer with a gain medium placed asymmetrically in the loop. By addition of pulses through the central coupler, the NALM (Non linear Amplifying Loop Mirror) transmits highest intensities of pulse and reflects the lowest ones [9-10]. The nonlinear fiber loop amplifies, shapes and stabilizes the circulating ultra short pulse [11]. With the P-APM (Polarization-Additive Pulse Mode-Locking), the polarization state of a pulse propagating through an optical fiber differs from the peak to the wings and the transmission through a polarizer can be adjusted to eliminate the wings [12-13]. The SAs act as intensity dependent elements. The wings of the pulse exhibit more losses than the peak [14].
Figure 10.
Figure of eight fiber laser.
The PCs (Polarization Controllers) set the input signal in an arbitrary polarization state. The azimuth and elliptical parameters define the polarization state of the output signal. Considering Einx and Einyas the polarization components of the input signal, the output signal is:
Where k is the power splitting ratio parameter and δyx(t) is the phase difference between the x and y components. The optical isolator is inserted into the loop to allow light circulate only in one direction. The major disadvantage of 8FL is that it requires a special management of the various parameters of the cavity [15]. In the steady state, the various linear and non linear effects are in balance and the pulse output power and width are unchanged or often even nearly constant after each completed round trip. Assuming a single circulating pulse, the pulse repetition rate corresponds to the resonator round-trip time.
In actively mode locked fiber lasers, as shown in Fig. 12, the pulses frequency depends on the electro-optic or the acousto-optic modulator inserted in the cavity [16-17-18]. Generally, these types of laser cavities provide typically pulses larger than those provided by a passively locked laser. This can be explained by the fact that no compression techniques are applied [19]. The most used optical modulator to actively mode lock the different modes oscillating into a fiber laser cavity is the MZM (Mach Zehnder modulator). It’s an intensity modulator based on an interferometer principle. It consists of two 3dB couplers which are connected by two waveguides of equal length (see Fig. 11). By means of electro-optic effects, an externally applied voltage can be used to vary the refractive indices in the waveguide branches. The different paths can lead to constructive and destructive interference at the output, depending on the applied voltage. Then the output intensity can be modulated according to the voltage. A Mach Zehnder Modulator has often only one optical exit, the second one is hidden.
Aiming to profit at the same of the two configurations advantages: a rather low width and a sufficiently high repetition rate of pulses, new prospects and configurations of fiber lasers, using both the passive and active mode locking techniques, have been proposed. This new generation of pulses generator is called hybrid type mode locked fiber laser [20]. Fig. 13 shows hybrid type mode locked fiber laser using both a machZehnder modulator to actively mode lock the cavity and a non linear amplifying loop mirror to passively mode lock the cavity.
Figure 13.
Hybrid type mode locked 8FL.
Being 90% made of fiber; light propagation through a fiber laser can be modeled by the Split Step Fourier Method.
5. Split step fourier method
Light propagation within optical fiber may be expressed by the Generalized Non Linear Schrödinger Equation (GNLSE) as follow:
β2 and β3 are the second and the third order dispersion terms, α is the attenuation coefficient of the fiber, T is the related time given by T=t – z/vg where z and vg are the longitudinal coordinate and the group velocity corresponding to the central wavelength λ and γ is the nonlinear parameter of the fiber given by γ=2πn2/ λAeff. n2 is the non linear refractive index and Aeff is the effective area of the fiber. When studying the propagation into an EDFA, the GNLSE become:
The SSFM relies on that propagation in each segment of the optical fiber is divided in three steps: two linear and one non linearsteps (see Fig. 14). The nonlinear step is inserted between the two linear steps [21-22].
Figure 14.
Principle of Split Step Fourier Method SSFM.
So, linear and nonlinear effects are supposed to be applied in the whole segment of the fiber. The linear operator is used in the frequency area and the non linear one is used in time area.
The EDFA is based on a two-level Er3+ system assumption that is usually adapted to model erbium-doped fiber amplifiers. The lifetime transition from level 4I11/2 is of the order of microseconds for silicate hosts. Therefore, it is reasonable to neglect the population density N3 in the rate equations description. A two-level system approximation is used in this case. Under the assumption of the normalized population densities N1 and N2 at the ground and metastable energy level, 4I15/2and 4I13/2 populations are calculated by numerically solving the rate and propagation equations [23]:
Where the optical powers are expressed in units of number of photons per unit time, τ is the metastable spontaneous emission lifetime, N is the number of channels taken into account in the simulation (including signals, pumps, and ASE bins), ρ is the number density of the active erbium ions, α is the attenuation coefficient (which takes into account the background loss of the fiber), Δν is the frequency step used in the simulation to resolve the ASE spectrum, and Aeff is the effective doped area given by πb2, where b is the Er doping radius (it is considered a uniform distribution of erbium ions in the area given by the Er doping radius region). The nth channel of wavelength λn has optical power Pn(z,t) at location z and time t, with emission and absorption cross-section σne and σna respectively, and confinement factor Γn. The superscript symbols + and – are used respectively to indicate channels travelling in forward (from 0 to LEDFA) and backward (from LEDFA to 0) directions. For beams travelling in the forward direction un=1 and for beams in the opposite direction un=-1. The overlap integrals Γn between the LP01 mode intensity distributions doped region areas are given by:
Γn(ν)=∫0b|E(r,ν)|2rdr∫0∞|E(r,ν)|2rdrE10
7. Interaction between mode locking mechanism and non linear effects in fiber laser
Normally, when designing extremely high output average and peak power fiber laser generating ultra short pulses, the best solution that can be adopted is to enhance the non linear effects in the cavity. This can be achieved either by pumping the piece of doped fiber amplifier with a high input power rate or enhancing the SPM, XPM and FWM effects by reducing the average dispersion of the cavity and the effective area of the different fibers used. In this section, managing the pumping input powers level, the dispersion and the effective area of different microstructured optical fibers inserted into a passively and an hybrid type mode locked 8FLs, we prove that enhancing non linear effects does not lead necessarily to better results. It depends also on the type of mode locking mechanism used. The highest peak powers and the narrowest pulse widths are obtained only for specific parameters.
In spite of their singularities and particularities in managing linear and non linear effects, the exploitation of MOFs in laser cavities has remained a subject of research bit addressed. In fact, MOFs offer many degrees of freedom in the management of dispersion and effective area
A schematic diagram of the first passively mode locked 8FL is shown in Fig.15. It consists of two loops: a ring cavity and a non linear amplifying loop mirror NALM connected to each other through a 50% central coupler. The linear cavity is made up of 10m of PDF (Positively Dispersive Fiber: β2=20ps2/km) having 85µm2 as effective area and aiming to maintain balance between anomalous and normal dispersion within the 8FL, a 10% output coupler and a polarization insensitive optical isolator to ensure the circulation of light only on the clockwise direction. The NALM includes a MOF (Microstructured Optical Fiber) and a 10m EDFA (Erbium Doped Fiber Amplifier) having 0.24 as numerical aperture forward and backward pumped by two 980nm pump laser diodes coupled to the loop through two 980/1550nm WDM couplers. The Er3+ ions density is 700ppm.
Figure 15.
Configuration of passively mode locked 8FL.
The second configuration, shown in Fig.16, is a hybrid type mode locked 8FL. It differs from the first one by the presence of a MZM (Mach Zehnder Modulator) as an electro-optical modulator into the linear ring cavity.
Figure 16.
Configuration of hybrid type mode locked 8FL.
By modelling the light propagation through the various components by the SSFM (Split Step Fourier Method), we studied the influence of varying nonlinear parameters of the cavity on the output pulses shape. Light pulse propagation in the 8FL may be expressed by the NLGSE (Non Linear Generalised Schrödinger Equation) and the transfer function of the different components used [12]. The central coupler is a cross-coupler for combining or splitting the optical signal. It is bidirectional, with wavelength independent coupling, insertion loss and return loss. If we consider Ein, Eout, E3 and E4 respectively the input, transmitted, NALM clockwise and counter clockwise circulating light powers, after propagating into an L length loop made of EDFA and MOF, considering only the non linear effects, E3L and E4L are expressed as follow:
Where k is the power splitting ratio parameter, G is the EDFA gain, λ is the signal wavelength and Aeffis the MOF effective area. For each round trip through the fiber laser, the transmitted power circulating into the ring linear cavity is:
A single secant hyperbolic input pulse with 1mW of peak power and 200ps FWHM (Full Width at Half Maximum) is launched in the first configuration through the WDM coupler. At the beginning, we studied the output pulses shape for different EDFA pumping power levels and differ4ent MOF effective area’s values. The pumping threshold is about 300mW. In fact, as illustrated in Fig.17 and Fig.18 below, when increasing the pump power of the EDFA, the pulses peak power increases whereas the width decreases. However for very small effective areas like 5µm2 and 10µm2, the pulse width reaches a minimum value at a specified pump power level before growing up proportionally to the laser diodes pump powers. In these cases the lowest values of the pulse width are reached respectively for 400mW and 700mW of pump powers.
A second approach to study the non linear effects impact in a fiber laser cavity is to use longer portion of the non linear optical fiber used. Fig.19 and Fig.20 show the output pulses peak power and width for different lengths and effective areas of MOF. The pump power delivered by each laser diode is equal to 700mW.
As shown in Fig.19 and Fig.20, enhancing dramatically the non linear effects, by increasing the MOF length and decreasing its effective area, does not lead necessarily to optimal results. In fact, for each length of one selected fiber there are two optimal effective areas. The first corresponds to the one leading to the highest peak power and the second corresponds to the one leading to the lowest pulse width and conversely. However, there is always an intermediate value of the effective area leading to a high peak and a low pulse width. For 10m of MOF, the intermediate effective area is 7.5µm2. The peak power is equal to 16W and the pulse width to 39.7ps. However, the highest peak power 18.25W and the lowest pulse width 39ps are obtained respectively for 5µm2 and 10µm2 effective areas. For 20m of MOF, the intermediate effective area is 15µm2. The peak power is equal to 17.25W and the pulse width to 38.5ps. However, the highest peak power 20W and the lowest pulse width 37.5ps are obtained respectively for 10µm2and 17.5µm2effective areas. For 30m of MOF, the adequate effective area is 15µm2.
Thus, by reducing the mean dispersion of the cavity with an appropriate choice of the MOF optimal length and effective area, generated ultra short pulses would have the highest peak power and the lowest width.
Unlike the passively mode locked 8FL carried out above, in case of hybrid type 8FL shown in Fig.16, no input pulse is inserted in the cavity to release the cavity oscillation. The first handling aimed to study the average pulses output power fluctuation according to the pump powers of the two lasers diode for different MOF’s effective areas. The MOF length and dispersion are respectively 30m and -10ps2/km. The PDF length and dispersion are respectively 10m and 20ps2/km with an effective area of 85µm2. The electrical signal frequency injected into the MZM is 20GHz. As shown in Fig.23, more the effective area is small and the pumping powers are high more the mean power of output signal is high. So, by increasing non linear effects, we increase the output pulses power.
Figure 17.
Peak power vs launched pump powers (LMOF=10m, β2MOF=-10ps2/km).
Figure 18.
Width vs launched pump powers (LMOF=10m, β2MOF=-10ps2/km).
Figure 19.
Peak power vs MOF’s effective area and length.
Figure 20.
Width vs MOF’s effective area and length.
About pulses shape depending on group velocity dispersion, Fig.21 and Fig.22 show that the best results correspond to MOF having negative chromatic dispersions.
Figure 21.
Peak power vs MOF chromatic dispersion.
Figure 22.
Width vs MOF chromatic dispersion.
Figure 23.
Mean power vs launched pump powers.
The repetition rate and the width of output pulses are fixed by the electro-optical modulator characteristics.
The repetition rate of pulses depends directly on the frequency of the electrical signal injected into the MZM. Fig.24 illustrates the variation of the width of output pulses according to the electrical signal frequency.
Figure 24.
Width vs Repetition rate.
Fig.25 shows hybrid type output pulses with a repetition rate of 20GHz.The second handling aimed to study the average pulses output power fluctuation from a hybrid type 8FL according to non linear effects by varying the length and the effective area of the MOF.
Curves shown in Fig.26 illustrate that more the MOF is long and its effective area is small more the exit power of the laser is significant. However, a significant increase of the MOF length and the effective area leads to a fast power fall. We can also notice that for all different MOF’s lengths there is a particular value of the effective area leading always to the same result. In this case, it corresponds to 12µm2.At the end, we studied the hybrid type 8FL behaviour when decreasing the average chromatic dispersion of the cavity. Contrary to passively mode locked 8FL, the maximum values of exit power, for a hybrid type 8FL, are reached for normal dispersion of the MOF β2MOF>0 (see Fig.27).
Figure 25.
GHz hybrid type 8FL output pulses.
Figure 26.
Mean power vs MOF length and effective area (β2MOF=-10ps2/km).
Thus, increasing the average exit power of hybrid type 8FL, operating at any pulses repetition rate, can be reached by choosing a rather long MOF having small effective area and normal dispersion.
Figure 27.
Mean power vs MOF chromatic dispersion.
8. Conclusion
We summarized different techniques used to generate ultra short pulses from a fiber laser. Using the Split Step Fourier Method algorithm to model light propagation within a loop cavity, we described some operating process of different kind of mode locked fiber lasers. We also focused on some optical components operating process used in fiber laser to passively or actively mode lock the different modes oscillating within a laser cavity. In addition, we focused on Erbium Doped Fiber Amplifier operating process. We highlighted the improvement of fiber laser performances does not depend only on the management of the non linear parameters of the cavity. In fact, it depends tightly on the mode locking mechanism used. A passively mode locked 8FL and a hybrid type 8FL do not respond the same way to non linear effects increase. In fact, in case of passively mode locked 8FL, for each length of the high non linear fiber, correspond two associated optimal effective areas: one leading to the highest peak power and one leading to the lowest pulse width. Whereas, increasing the non linear effects by using a rather long high non linear fiber having a reduced effective area leads to the best output results in case of hybrid type 8FL. Moreover, contrarily to hybrid type 8FL, reducing the average dispersion of the cavity leads necessarily to better output passively mode locked 8FL pulses shape. In fact, this work aims to illustrate the existing interaction between non linear effects and mode locking mechanism in fiber laser.
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Introduction",level:"1"},{id:"sec_2",title:"2. Q-switching mechanism",level:"1"},{id:"sec_3",title:"3. Mode locking mechanism",level:"1"},{id:"sec_4",title:"4. Pulsed fiber laser",level:"1"},{id:"sec_5",title:"5. Split step fourier method",level:"1"},{id:"sec_6",title:"6. Erbium doped fiber amplifier",level:"1"},{id:"sec_7",title:"7. Interaction between mode locking mechanism and non linear effects in fiber laser",level:"1"},{id:"sec_8",title:"8. Conclusion",level:"1"}],chapterReferences:[{id:"B1",body:'LiuJ.ShenD.TamS. C.LamY. L.Modelling“.pulseshape.ofQ-switched.lasers”I. E. E. E.Journalof.QuantumElectronics.vol.3p.888 EOFJuly 2001'},{id:"B2",body:'K. Merzouk, “Etude d’un système bas coût de transmission optique par multiplexagetemporel”, Thèse de doctorat, InstitutPolytechnique de Grenoble, France, Avril 2008.'},{id:"B3",body:'SalhiM.HabouchaA.LeblondH.SanchezF.Theoretical.studyof.figure-eightall.fiberlaser. .PhysRev. A, 77, 033828, (2008'},{id:"B4",body:'ZhengZ.IqbalM.YuT.Cavity“.Dynamicsof. a.Figureof.EightFiber.Laser”International.Journalof.Communications2007'},{id:"B5",body:'TarekEnnejah.FaouziBahloul.RabahAttia. “.Generationof.NonUniform.Pulsesby.anEight.MicrostructuredOptical.FiberLaser”. J.Optical. Communications, 321072011'},{id:"B6",body:'TheimerJ.HaustJ. W.Figure“.eightfibre.laserstable.operatingregimes”.Journalof.modernOptics.919 EOF928 EOF1997\n\t\t\t'},{id:"B7",body:'HoferM.OberM. H.HaberlF.M. E.Fermann “Characterization of ultrashort pulse formation in passively mode-locked fiber lasersIEEE J. Quantum Electron. 28 (3), 720 EOF728 EOF1992'},{id:"B8",body:'[7]M. E.Fermann“.Passivemode.lockingby.usingnonlinear.polarizationevolution.ina.polarizationmaintaining.erbium-dopedfiber”.OpticsLetters.894 EOF1993'},{id:"B9",body:'RichardsonD. J.LamingR. I.PayneD. N.MatsasV.PhillipsM. W.Self“.startingpassively.modelocked.erbiumfiber.ringlaser.basedon.theamplifying.Sagnacswitch”.ElectronLett. 27 (6), 542 (1991'},{id:"B10",body:'YoshidaE.KimuraY.NakazawaM.Femtosecond“.ErbiumDoped.FiberLaser.withNon.linearPolarization.Rotation”Jpn.J. Appl. Phys. 33 (10), 5779 (1994\n\t\t\t'},{id:"B11",body:'DulingI. N.All-fiberI. I. I. “.ringsoliton.lasermode.lockedwith. a.nonlinearmirror”.OpticsLetters.539 EOF1991\n\t\t\t'},{id:"B12",body:'TheimerJ.HaustJ. W.Figure“.eightfibre.laserstable.operatingregimes”.Journalof.modernOptics.919 EOF928 EOF1997\n\t\t\t'},{id:"B13",body:'ZhengZ.IqbalM.YuT.Cavity“.Dynamicsof. a.Figureof.EightFiber.Laser”International.JournalOf.Communications2007\n\t\t\t'},{id:"B14",body:'KuzinE. A.Andrade-LucioJ. 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Jeon, S.Y. Park, H.K. Lee, and E.H. Lee, “Gain Dependent Optimum Pulse Generation Rates of a Hybrid-Type Actively and Passively Mode-Locked Fiber Lase”, ETRI Journal, vol.n°. 1, 1April 1996'},{id:"B21",body:'G.P. Agrawal, Nonlinear Fiber Optics, 2nded, 1995'},{id:"B22",body:'G.P. Agrawal, Applications of Nonlinear Fiber Optics, 2001.'},{id:"B23",body:'GilesC. R.DesurvireE.Modeling“.erbium-dopedfiber.amplifiers,”Journal.ofLight.waveTechnology.VolN. 2, 2712831991'}],footnotes:[],contributors:[{corresp:null,contributorFullName:"Tarek Ennejah",address:null,affiliation:'
Unité de Recherche Composants et Systèmes Electroniques UR-CSE, Ecole Polytechnique de Tunisie, EPT, La Marsa, Tunis, Tunisie
Unité de Recherche Composants et Systèmes Electroniques UR-CSE, Ecole Polytechnique de Tunisie, EPT, La Marsa, Tunis, Tunisie
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1. Introduction
The phylum Nematoda consists of non-segmented invertebrates commonly known as roundworms that occur in a wide range of habitats around the globe and lack jointed appendages. The causative agent of trichinellosis is Trichinella species including Trichinella spiralis (T. spiralis) which belongs to the superfamily Trichinelloidea of the phylum Nematoda. At present, the recognized number of species and genotypes in the genus Trichinella are nine and three respectively based on the larvae appearance in muscle cells [1, 2]. The most pathogenic and prevalent pathogen in this genus is T. spiralis [3]. This is a most serious zoonotic food-borne parasite which can infect a wide range of hosts, is liable for trichinellosis disease in humans, and can infect more than 150 species of animals such as carnivores and omnivores including human beings worldwide [4, 5]. Trichinellosis occurs around many parts of the world and infects a huge number of human beings. Its ranges from Europe, North America, China, Japan, and Tropical Africa. However, China is the most affected country [1, 6].
The definitive hosts of nematode Trichinella include many domestic animals such as pigs, horses, and wild animals like bears, rats, and wild pigs. Each intermediate host of Trichinella spiralis is also its definitive host and serves as a source of infection for any other definitive host species by carnivorism [2]. Humans can acquire infection by the ingestion of undercooked, or raw meat of these animals contaminated with the larvae of T. spiralis [7]. The important preventive measure (to limit people from getting trichinellosis) is to disrupt the transmission of infective Trichinella larvae encapsulated in meat to human beings [8, 9]. However, in some countries, this disease is also transmitted by wild animals [10]. Since an enormous amount of pork and its by-products are consumed in China, that results in increased issues in the country [5, 11]. Unlike many other helminth parasites, the survival of T. spiralis nematodes is only direct host-to-host transmission adapting normal cellular functions and host immunity at all the stages of infection [12, 13].
The life cycle of T. spiralis starts when an adult female and male worms reproduce sexually in the small intestine of the host. The unique characteristic of T. spiralis among other parasites is that it passes all the stages of its life cycle within a single host including all phases of adult worm, newborn larvae, and muscle larvae [10, 14, 15]. Humans acquire the infection when they ingest Trichinella larvae that are encapsulated in the striated muscles of domestic or wild animals [5]. After the consumption of infected meat, parasitic larvae which are encysted in the meat are then released into the host stomach by acid-pepsin digestion [16]. Columnar epithelial cells of the intestine at the base of the villus are invaded by muscular larvae. Previously released into the stomach from meat and each molts four times to reach sexual maturity [7, 9, 17]. Approximately 1500 newborn larvae are produced by each fertilized female T. spiralis within 2–3 weeks and up to 10,000 over 4 months period. These larvae penetrate the intestinal lining with the help of unique sword-like stylet they possess and migrate to the striated (skeletal) muscles via the circulatory and lymphatic system [15]. These larvae can enter any type of cells but only survive in skeletal muscles. In the striated muscles, these previously migrated newborn larvae develop into infective muscle larvae and transform the skeletal muscle cells into a new type of cells known as nurse cells which maintain the larval life and for many species of Trichinella changes into capsules or cysts made up of hyaline and collagen fiber [8]. These capsules containing the muscle larvae can persist for many years and calcification occurs in most of the cysts and dies within a few months (see Figure 1). The life span of live adult worms in the mucosa of the intestine is 4–6 weeks in human beings while the muscle larvae encapsulated in the striated muscle fibers persist for months to years [1].
Figure 1.
Life cycle of T. spiralis [10].
Immunity is the defensive mechanism against any pathogenic organisms that invade the victimized host. When the host consumes contaminated meat containing nurse cells, an immune-mediated inflammatory response starts due to the development of the adult worms in the epithelium of the intestine to expel the parasites. The level of antibody IgE which defends the body against parasitic organisms starts to increase. The inflammatory infiltrates containing mast cells and eosinophils present there pathologically. Both these immune cells are involved in the clearance of parasites. Toxic oxygen molecules and major basic proteins are elaborated by eosinophil to kill invaded organisms but also cause to damage the host body tissues. While mast cell protease-1 (MCP-1) is produced by mast cells that are also lethal to worms. There is widespread inflammation, edema if worm load is high and cells death occurs frequently during the parenteral stage of infection [18, 19].
Trichinellosis infection is classified into three stages depending upon the life cycle of the pathogenic worm; (1) as an invasive stage, in which larvae grow into adult worms and after fertilization females begin to release newborn larvae which then migrate to blood circulation via the Lymphatic system [9]. This stage is characterized by nausea, diarrhea, abdominal cramps, and seldom vomiting. Constipation is also seen in some of the individuals instead of diarrhea. All these symptoms appear within 2–30 h of post-eating infected food. (2) As migratory phase; characterized by the encapsulation of larvae in the muscles of the host. The main symptoms observed in this stage include fever, face edema, swelling, muscle pain, and weakness of the infected muscles [14, 16]. (3) As encystment stage; characterized by the calcification of cysts in the striated muscles only and results in everlasting injury [1]. As this parasite shows nonspecific signs and symptoms of the disease, its clinical diagnosis is difficult [15]. For the diagnosis of Trichinellosis, the digestion method is the best method reported by World Organization for Animal Health (OIE) but to better detect the Trichinella parasites molecular biology and serologic methods have been developed [5]. Currently, Trichinellosis diagnosis is based on larvae detection in muscle biopsy or immunodiagnostic tests which are highly specific. Many antigens are expressed during the developmental stages of the T. spiralis and are useful for the serodiagnosis of trichinellosis. However, due to limited T. spiralis antigens availability testing is not extensively available [15, 16].
Trichinellosis is not only responsible for public health casualties but also cause the economic problem in food safety and swine animal production. Due to a large number of people infected with T. spiralis, this disease is regarded as re-emerging in many regions of the world [2, 15]. If transmission of this disease is not under control, it can lead to serious public health problems [10].
2. Status of anti-T. spiralis vaccine
It is a promising method for the control of parasites in pigs to develop a vaccine against T. spiralis infection. However, most of the studies for the development of vaccines against Trichinella have been performed in lab animals (Mouse models) so far. Only a few studies are performed on pigs for the development of vaccines against Trichinella infection. To prevent and control the transmission of T. spiralis infection from pigs to humans vaccine exploitation is an important step [20]. Trichinella is a tissue-lodging, enteral, and multicellular parasite. Its life cycle is complex and has a diverse developmental phase. Trichinella worms have stage-specific antigens [21]. It is necessary to develop an effective vaccine against Trichinella to interrupt the transmission of parasites among animals and the cycle of pathogen transmission from swine to humans [5].
Till now, various practices and strategies have been used in the prevention and eradication of parasites including the application of chemicals. The chemical methods are not well signed and have certain limitations such as continuous use of antiparasitic drugs resulting in rising resistance, have risks related to the environment, health, and their potential effects on the host or non-target organisms. Chemotherapy and antiparasitic drugs are used to prevent T. spiralis infection, but when we compare the vaccination of animals with chemotherapy treatment, we found that it has several advantages. A single dose of vaccine can provide lifelong prevention of T. spiralis infection, reduce the risk of drug residues in meat and other by-products, and decline the emergence of T. spiralis drug-resistant parasites [2].
Anti-Trichinella vaccines will provide a substantial contribution to the control, prevention, and elimination of Trichinellosis. The eradication of Trichinella spp. infections in animals is a difficult task as vaccines against Trichinella that act as a preventive weapon are not currently available except for rats and pig models [6]. For the past three decades, significant improvements have been made for the recognition of several antigens from T. spiralis. It will lead toward a better understanding of the formulation of novel vaccine developments. A variety of vaccines such as subunit vaccines, recombinant proteins vaccines, inactivated vaccines, synthesized epitope vaccines, DNA vaccines, viral or bacterial vector vaccines can elicit an immune response against Trichinella and provide effective protection. Scientists have used different antigens to formulate recombinant protein vaccines and many of them have provided some effective protection against Trichinella infection [4, 10].
Preventive vaccine development against Trichinella infection in domestic pigs is valuable to control and prevent this parasite [21]. The diseases can also be controlled in animals through a veterinary vaccine. To induce long-term intestinal immunity the appropriate route for immunization against Trichinellosis is oral as the infection occurs due to the ingestion of poorly cooked meat containing encapsulated infective larvae [20]. Proteases (enzymes) are widely distributed in viruses, prokaryotes, and eukaryotes participate in different events of the parasite’s life cycle. In the process of causing infection, parasites serine proteases are thought to be a key factor and exist in the T. spiralis excretory-secretory (ES) products [20, 22]. The hydrolyzing enzyme Elastase (trypsin-like serine protease) helps the parasites in the penetration of host tissue through the hydrolysis of laminin, fibronectin, elastin, and type IV collagen. Elastases also participate in the immune evasion process. This enzyme is also involved in the digestion and molting of parasites and has an important role in parasitic worm intrusion of victimized hosts. It might be a target for a novel vaccine [21]. Recently, many anti-T. spiralis vaccines have been developed to interrupt the transmission of parasites from animals to humans. Many vaccine candidates which are effective against T. spiralis were selected from ES products and recombinant proteins. Serine protease enzymes (from T. spiralis) provide partial protection against T. spiralis larvae challenges [20].
T. spiralis exerts an immunomodulatory effect through ES products on the immune response of the host [22]. T. spiralis Nudix hydrolase (TsND) is a protein that binds to intestinal epithelial cells of normal mice (up-regulated gene). The size of this gene is about 1248 bp. A partial protective immunity against Trichinella infection was observed when mice were vaccinated with recombinant TsND protein [22, 23]. Vehicle (delivery system) and antigen are important elements that are responsible for the protection level induced by the candidate vaccine [24].
3. T. spiralis -associated immune mechanism
T. spiralis (Helminth) establishes infection which is long-lasting in the striated muscles of the host. Depending on the longevity of the host. It can persist successfully until the end of life in rodents and in higher species including humans can persist over several months to years following infection. They do not kill the host striated muscles cells during their stay, unlike some other intracellular parasites. This characteristic makes them one of the most successful symbiotic parasites [25]. Parasitic nematode T. spiralis completes its entire life cycle in one host and each stage of its life cycle provokes the immune system of the host differently [14, 15]. For the establishment of the life cycle successfully, this parasite with the help of its defensive mechanism manages to escape the host immune system responses. The excretory-secretory products of T. spiralis play a crucial role in the establishment of parasitism and modulation of host immune response to protect both host and the parasite [22]. When the host acquired the infection of T. spiralis, at early-stage cellular immunity of the host is inhibited but later recovery of host cellular immune function occurs, and humoral immunity starts its role in resisting the infection of T. spiralis. During the infection, both the cells Th1 (T helper cell) and Th2 play a major role in maintaining the immune system function. They are involved in the eradication of pathogens. When the maintenance of the host immune system disrupts it gets infected [18]. Nitric oxide (NO) is a molecule in the immune system which acts as an immunomodulator and immunotoxin. It is a gaseous molecule and has appropriate lipid membranes solubility. Without binding to any specific receptor of viruses and bacteria, it exerts lethal effects on them. NO is involved in the selective killing of parasites including infected cells and has a major role in the adult worm expulsion during Trichinella spiralis infection in mice (see Figure 2) [12].
Figure 2.
Immune response against T. spiralis.
Infection of T. spirilis has an immunosuppressive effect on the innate immune system of the host. Larvae release secretory antigens that elicit a protective strong immune response which is specific to invading parasites [20]. ES products reduce inflammation when parasites invade the muscle cells, modulate the host immune system response in a way to protective for both the host and the parasites. For survival inside the organism, T. spiralis build a unique place for their living and their niche contains a cyst or capsule composed of nurse cell (cellular components) and collagenous wall [22, 25, 26]. Both the wall and nurse cell are originated from the host, provide protection and maintenance of the parasite’s metabolism respectively [14].
Macrophages play a major role in the immune response of the host against various pathogens [22]. In vitro, ES products from different phases of the life cycle of T. spiralis can modulate macrophage’s function by inhibiting cytokine production. In chronic helminth infections, macrophages are activated by Th2 cytokines such as interleukin-4 (IL-4) and interleukin-13 (IL-13). Many immune mediator molecules are released such as IL-6, IL-12, nitric oxide (NO), and tumor necrosis factor (TNF-α) when in macrophages the signaling pathways are triggered by Th2 cytokines [22, 25, 27].
4. Genomic and proteomic profile of T. spiralis
There are 12 species and genotypes of Trichinella which are distributed worldwide and cause serious disease Trichinellosis in humans which leads to morbidity and mortality [1, 2, 12]. Based on larvae appearance in the muscle cells of the host only encapsulated and non-encapsulated clades (morphological distinct) Trichinella is recognized. Based on molecular studies, nine species and three genotypes of Trichinella show a wide biological diversity. Based on genetic data, only Trichinella encapsulated clade infects mammals includes Trichinella spiralis (T1), Trichinella nativa (T2), Trichinella britovi (T3), Trichinella murrelli (T5), Trichinella nelson (T7), and Trichinella patagoniensis(T12). The three Trichinella genotypes includes T6, T8, and T9. The Trichinella non-encapsulated clade includes Trichinella pseudospiralis (T4), which infects birds and mammals only, Trichinella papuae (T10), and Trichinella zimbabwensis (T11), they infect reptiles and mammals [1].
Proteomics (because of bioinformatics and mass spectrometry) is an effective technique to examine the modifications after the translation of genes such as proteolysis or glycosylation. These are powerful techniques to examine the samples obtained from pathogens to find the possible proteins involved in the pathogenesis of the disease [12]. Trichinella is substantially different in molecular and biological characteristics from other crown groups. The assembly of Trichinella is 64 million bp in length and about 15,808 proteins are encoded by this genome assembly. In T. spiralis genome, the estimation of repeat content is about 18% having low GC content (about 27%) relative to the overall genome (34%) and protein-coding region (43%) of Trichinella spiralis[14]. Microsatellites are present in the entire genome and many are distributed in the non-coding sequence of the genome. It leads to genetic diversity due to mutation [28]. During the early stage of Trichinella infection, Trichinella spiralis 14-3-3 protein is a strong immunogenic antigen [29]. Ts14-3-3 is an immunodominant antigen and this protein is also used to detect the whole period of infection with Trichinella. During the early phase of Trichinella infection, HSP70, cysteine protease, and Ts14-3-3 play a crucial role in balancing the host–parasite relationship. Therefore, these proteins are a good target for the development of vaccines and early immunodiagnostic measures [15].
4.1 DNA based vaccine
DNA vaccines got a glare in the early 1990s and evoked both humoral and cellular responses, when tested and identified, particularly induced cytotoxic T cell response, and abolished the safety concerns associated with the live vaccine [17]. Such vaccines tend to sustain host immune system stimulation in comparison to the Recombinant protein-based vaccines [6]. DNA vaccines emerged as a strong way of eliciting a humoral and cellular immune response against many parasitological antigens in small animal models. Moreover, DNA vaccines produce a concurrent Th1 and Th2 immune response against T. spiralis [30, 31].
The TspE1gene encoding a 31 kDa antigen of T. spiralis has been cloned to an expression vector pcDNA3 and administered in a mouse as a DNA vaccine [31]. Naturally, T. spiralis challenge suppresses the type 2 immune system response which inhibits them [17]. The mice immunized with the TspE1-pcDNA3 presented a significant larval reduction rate and an increased serum anti-Trichinella antibody level, hence this DNA vaccine proved to be partially protective against T. spiralis challenges [31]. Spleen cells after stimulation with the TspE1 recombinant protein exhibited a lymphoproliferative response, which is an indication of cellular response elicited by the DNA vaccine. Sequence of a serine protease (Ts-NBLsp) cDNA from newborn larvae of T. spiralis, cDNA sequence of recombinant TsNd (Trichinella spiralis nudix hydrolases) has been cloned to the plasmid pcDNA3.1 [17, 31, 32]. The antibody response against the serine protease of T. spiralis inhibits the protease activity thus hindering invasion of the parasite. The DNA vaccines Ts-NBLsp-pcDNA3.1 and pcDNA3.1-TsNd presented a balanced systemic Th1\\Th2 immune response. The immunization with recombinant TsNd DNA vaccine resulted in an increased intestinal IgA and total IgG response with an exalted IgG1 than that of IgG2a [31]. To compare the recombinant nudix hydrolase DNA vaccine, the Ts-NBLsp-pcDNA3.1 vaccine showed a dominant IgG2a anti trichinella antibody and a predominantly Th1 immune response [17]. DNA vaccines elevated IFN gamma, IL-2, IL-4, and IL-10 levels [31]. Secretory IgA causes a significant reduction in the female worm fecundity and this response is enhanced by cytokine IL-10 specifically. The intestinal mucosa of the infected animals produces a specific antibody response against T. spiralis. Ts-NBLsp-pcDNA3.1 and reduces the muscle larvae burden (77.93%) greater than that of the TsNd vaccine (53.9%).
In another study of the TsDNase II, the complementary DNA sequence of T. spiralis serine protease 2.1 has been cloned to the eukaryotic expression vector pcDNA3.1 and administered as a DNA vaccine through an attenuated Salmonella typhimurium to avoid degradation [30]. To elicit a persistent systemic and mucosal immune response against T. spiralis, attenuated salmonella is an effective live carrier that gives an efficient mode of vaccination. T. spiralis DNase II is an excretory-secretory product associated with adult worms and IIL which is expressed in the cuticle of IIL. T. spiralis serine protease appeared to be present in the spliceosome and cuticle of adult worms and intestinal infective larvae. Both of these vaccine candidates against T. spiralis resulted in the significant rise of specific IgG responses. IgG1 titer after the first dose of vaccination and then an increased level of IgG2a after the second dose of vaccination, furthermore they produced mixed Th1\\Th2 response which can be described through elicited cytokines response as Th1(IFN gamma) and Th2 cytokines (IL-4, IL10) [30]. TsSP 1.2-pcDNA3.1 vaccine resulted in a 71.84% reduction in the muscle larvae in comparison to the TsDNase II DNA vaccine which caused a 59.26% reduction in the muscle larvae [30].
T. spiralis adult-specific DNase II-1 (TsDNase II-1) and DNase II-7 recognized in the excretory-secretory proteins of the AW [30] has been analyzed for their immune response against the worm. Antibody-dependent cell-mediated cytotoxicity assay (ADCC) revealed that both recombinant anti-TsDNase II-1 and anti-TsDNase II-7 sera mediated the attachment of mouse peritoneal exudate cells (PECs) to NBL and finally killing of the NBL. Paramyosin is a thick myofibrillar protein [6, 30, 33], which is an immunomodulatory protein that evades host immune response by inhibiting complement C1q and C8\\C9. TsPmy and Ts87 both are efficient vaccine candidates against T. spiralis. The DNA encoding TsPmy and Ts87 have been cloned in a eukaryotic vector pVAX1 and the recombinant DNA was transformed in the S. typhimurium strain SL7207. The resulting DNA vaccines produced protective immunity against T. spiralis when administered in mice, both resulted in mucosal sIgA response in the intestine and systemic anti TsPmyIgG response. The antibody-secreting cells from the spleen and mesenteric lymph nodes of the mice immunized with TsPmy vaccine expressed the intestinal homing receptors CCR9 and CCR10. [30] determined that SL7207\\ pVAX1-TsPmy vaccine came out with a 44.8% reduction in muscle larvae and a 46.6% reduction in adult worms. While SL7207\\ pVAX1-Ts87 caused a 34.2% reduction in muscle larvae and a 29.8% reduction in adult worm burden. By using B and T cell epitopes from TsPmy a novel multi-epitope vaccine has been designed which elicits an immune response more efficiently as compared to traditional vaccines, TsPmy MEP vaccine reduced the muscle larvae up to 55.4% [33].
DNA vaccines have many advantages as they are inexpensive, focused immune response against the antigen of interest, heat stable and a broad-spectrum vaccine can be developed by mixing plasmids.
4.2 Protein-based vaccine
In recent studies, it is reported that specific protein molecules from numerous T. spiralis life cycle stages have been considered and expressed properly, so that their immune protection was also estimated, such as paramyosin (Ts-Pmy) obtained from an adult cDNA library [21], TspGST and fructose-1,6-bisphosphate aldolase (Ts-FBPA) taken from the T. spiralis draft genome utilizing high expression at the ML stage, Ts31 from the ML ES proteins, serine protease (TsSP) from IIL (intestinal offensive stage) and ML surface proteins and cathepsin B (TsCB) from the T. spiralis draft genome [8]. On the other hand, when these recombinant proteins were used for vaccinating mice, they showed only 36.2–53.50% ML reduction following the T. spiralis challenge. In the current study, we determine the protective immunity persuaded by vaccination through a novel TsE protein. TsE is highly expressed and acts as a secretory protein at the T. spiralis intestinal offensive stage (IIL), TsE shows potency to be exposed first to the host’s intestinal mucosa and then produce the local immune response through its working. It is observed that vaccination with rTsE persuaded significantly high levels of TsE-specific sIgA, which can simplify adult worm removal from the intestine. TE immune protection having 64.06% ML reduction, with this novel TsE vaccination was considered superior to those of the above-mentioned other T. spiralis proteins act as candidate vaccine target molecules. This study also recognized a foundation to develop polyvalent anti-T. spiralis vaccines in the upcoming period.
The immune response stimulated by a vaccine based on an exclusive antigen and multi-epitope (that work more efficiently than the large protein molecules) vaccines against T. spiralis has now been proposed. Therefore, the amalgamation of three selected epitopes from Ts-Pmy and Ts87 from T. spiralis adult produced in immunized mice IgG and IgG1 production and higher protection of about 35% in contrast to the parasite challenge in comparison to that encouraged by individual epitope peptides [8]. To achieve higher shielding immune responses counter to T. spiralis, it will be essential to propose a vaccine with multi-epitopes from different parasite stages focusing on NBL and adult stages (Table 1).
Antigen
Database ID
Strain
Developmental stage
Function
Reference
Cathepsin B [T. spiralis]
XP_003373289
ISS 195
Muscle larvae(ML) and adult worm (AW)
Has important function in worm invading, migrating, molting and immune escape
Immunoregulatory kinetics of different T. spiralis based protein after binding with host immune cells.
5. Role of progesterone receptor in trichinella spiralis
Progesterone (P4) is a sex steroid hormone that plays roles in the physiology of the reproductive system such as corpus luteum of the ovary and placenta in females, while testes and adrenal cortex in males also participate in many other functions such as brain activity, immune modulation, metabolism of bones heart and lungs physiology. P4 is also responsible for the maintenance of pregnancy and shows an immunosuppressive effect [35]. when a high level of progesterone is present during the luteal phase of the estrus/menstrual cycle in females. Recent studies showed that these hormones also influence the course of parasites infections and also restrict the invasion of parasites when a high level of P4 in female animals is produced. Restricts the invasion of parasites [11]. P4 has an immunomodulatory effect on fetal antigens during pregnancy by suppressing the maternal immune response. However, progesterone can be either an inhibitory or stimulatory effect on the immune response mechanism depending upon cell type, concentration, and exposure time to steroids. It has nematotoxicity against newborn larvae of T. spiralis. Progesterone is responsible for decreased parasite load during pregnancy [11].
Sex steroids are known as immune response modulators and play a major role in T. spiralis susceptibility at two levels viz. (1) protective immune response and (2) direct effect on the development of worms. Besides, P4 up-regulates many molecules expressions from major histocompatibility complex class I and it also participates in the down-regulation of genes that are responsible for the fecundity and oviposition of the worms and inhibits the nuclear factor kappa B (NFƙB) activation in innate immunity [11].
6. Role of progesterone and mifepristone against T. spiralis
Progesterone is a gonadal hormone primarily involved in the preparation of the endometrium for implantation of an embryo and necessary for the maintenance of pregnancy, while mifepristone is a drug that works as an antagonist of progesterone and glucocorticoid. It has an abortifacient effect and terminates early pregnancy by binding to intracellular progesterone receptors. Mifepristone has an antagonistic effect on the T. spiralis (Ts) membrane-associated progesterone receptor component-2 (Ts-MAPRC2). It also down-regulates the expression of the Ts-MAPRC2 gene and results in the abortion of the pregnant adult female worms [11].
Mifepristone (RU486) can be taken as an example that works as an antagonist in contrast to the progesterone receptor (PR) and glucocorticoid receptor (GR) with some lethal properties such as aborting agent and anticancer activities in the body. In the case of helminths, several research studies are concentrated on PGRMC receptors. Similarly, RU486 was one of the first medications accepted for surgical abortion and is frequently used to terminate an early or midterm pregnancy. Hereafter, PR and binding of P4 molecules (agonist) and RU486 (antagonist) can be helpful to elaborate T. spiralis species regarding differentiation and reproductive development as well as creating potential pharmacological targets that might be used as a drug therapy against Trichinellosis.
Progesterone is known for its immune-modulatory effects, which happen during pregnancy that is done by suppressing the response from the mother toward paternal antigens expressed in the fetus [11]. Taking into the description, we can conclude that progesterone is an adequate inducing activation of the effector cell populations responsible for cell death in an antibody-independent cytotoxic mechanism. This cytotoxicity should also be activated by soluble antigens released by the parasite because at constant self-aggression of tissues by these activated cells 0% NBL mortality 10 10 100 Progesterone (ng/ml) cells [35] .
7. Challenges to developing an efficient vaccine against T. spiralis
The control of helminths in animals is usually through anthelmintics. Vaccine development against T. spiralis infection in pigs is an alternate method for the prevention of parasite T. spiralis from zoonosis. Effective vaccine development against Trichinellosis is conducted in mice instead of pigs. Effective development of a vaccine, is not only due to high price of experimental pigs but also due to poorly satisfied antigens detected from the mice. Moreover, the immune response induced by the same antigen in swine and mice is extremely different. So, [2]. concluded that in mice, poor immunogenic vaccine candidates are not capable to induce a strong protective immune response against T. spiralis infection in pigs.
TsT was a T. spiralis somatic antigen and at adult-stage with specific surface antigen it had a good antigenicity. If vaccination of mice is done with TsT, it will induce a systemic mixed Th1/Th2 response and an intestinal local sIgA response, which can produce partial protection against T. spiralis larval challenge. Then these results suggested that TsT plays a role in T. spiralis growth and survival in the host, and it might be deliberated as a potential target antigen for anti-T. spiralis vaccines. However [9], revealed that oral anti-Trichinella vaccines comprised of multiple antigenic epitopes of various T. spiralis life cycle phases should be recognized.
7.1 Diversity within T. spiralis parasites
T. spiralis is a nematode parasite that is prevalent throughout the world and translocated by humans and their animals. They occupy well-defined geographic ranges [36]. There is a big diversity among the T. spiralis parasites present in different geographic locations [24]. T. spiralis nematode belongs to the clade that diverged early in the phylum Nematoda evolution [14]. T. britovi parasitizes many sylvatic mammals such as Felidae, Canidae, Ursidae, Mustelidae, Suidae, Viverridae and is endemic to Northern-western Africa and Eurasia while T. murrelli is the only present in wild animals in North America. Millions of years ago, Trichinella could infect human beings evidenced by the ingestion of other parasites in meat [36].
The nematode T. spiralis is involved in the most common cause of human trichinellosis, which is considered a zoonotic disease worldwide. The heredity of T. spiralis giving rise to the genus Trichinella and reported that the last shared common ancestor was approximately 275 million years ago (Lower Permian Period) identified, however the modification of extant Trichinella species happened about 16–20 million years ago [14].
We compare the molecular physiognomies of nematodes and former metazoans by using the T. spiralis genome as standard. This comparative approach by using the T. spiralis permitted us to categorize conserved protein and gene sequences through the superficial model, particularly for the phylum Nematoda. We bring an approach that intrachromosomal modifications were common all over the phylum. However, this was in divergence to other features such as births and deaths of a protein family, which exhibited clear discrimination among the parasitic and non-parasitic nematodes. The identification of well-maintained physiognomies predicated based on this work will advance the more accurate research on pathogens from a phylum embracing thousands of pathogens that are mainly to infect humans, animals, and plants and behaves like infectious agent. The advances possibly will one day be responsible for complete strategies to prevent and control diseases that are caused by pathogens from across the Nematoda family around the globe [14, 36, 37].
Commencing from the time of the discovery of Trichinella which is in 1835 in anticipation of the middle of the next century. During the last decade, the use of molecular and biochemical methods in combination with experimental studies on biology, have resulted in the identification of seven Trichinella species that have different epidemiological and topographical distributions. Even though these species are very difficult to differentiate morphologically, this can be done with the molecular and positive biological characters for further identification [16].
7.2 Genetic diversity related to multiple hosts
A total of 30 species of T. spiralis having mtDNA genomes has 20 unique haplotypes that were observed containing 86 isolating sites. So, with four out of five shared haplotypes taking place in European and North American samples. Samples from North America had one haplotype, which is present in each geographic sampling site [38]. Out of the total, mostly the variations were limited to the Asian T. spiralis samples. There are about 7 Asian samples, and from these 8 haplotypes were identified; these differed on an ordinary by 24.9 nucleotides. In comparison to this, western samples are averaged with only 3.2 nucleotide differences per haplotype with only 13 haplotypes in 23 samples; the most different pair of western haplotypes differed by only 6 nucleotide differences between any two isolates. Similarly, nucleotide diversity (pi) was 0.00016 in western samples while Asian nucleotide diversity was 10-fold greater (0.00179). As a result, we can say that all Asian samples are different from the western samples by at least 45 bp and averaged 49 bp differences [24].
The most noticeable properties of this parasite’s epidemiology are its requisite transmission by mode of meat ingestion in consumers. There is another important feature, which is present in two normally isolated ecological systems, which are sylvatic and domestic. In certain situations, the two biotopes are connected from end to end man’s activities, which results in the revelation of humans to Trichinella species [38]. Usually, it is restricted to sylvatic animals. The species furthermost often associated with human infection is T spiralis, which is the reported species that is usually found in the meat of domestic pigs. The life cycle of T spiralis includes a multipart set of possible routes. Farm transmission can be the result of predation on or hunting other animals for food purposes (rodents), hog anthropophagy, and the feeding of uncooked meat leftovers [16].
Most outbreaks resulting from ingesting of T spiralis infected pigs can lead to its outbreak through local single-source but, progressively, the mass marketing of meat can distribute the disease-causing parasite in the entire population. There has been a great increase in the reported cases due to Trichinella species, just because of having so many species that are involved in the food chain. The reason for having genetic diversity is also stated that we are lacking in vaccines to eradicate it. The main source is considered as the meat from the game and domestic animals. From recent reports, we can conclude that it also specifies that infected herbivores including horses, sheep, goats, and cattle have been the source of the outbreak [14, 16].
7.3 Multiple stage complexity of T. spiralis
T. spiralisalso has several stages of the complex life cycle that completes in two niches viz. intra-multicellular niche occurs in the intestine epithelium of host where adult male and female worms are involved with the help of (proteolytic digestive enzymes and become mature adult worms). Whereas intracellular niche occurs in the striated muscles of the organism where muscle larvae participate in the development of nurse cells [16], T. spiralis life cycle represents different antigens specific for a particular stage, where these antigens elicit immune responses and facilitate the developmental cycle of the parasites by modifying the host immune responses. To complete their life cycle, they skip the defensive mechanism of the host against invading the foreign body.
Once newborn larvae invade the lymphatic or circulatory system, they can drive anywhere in the organism and survive only in the skeletal muscles of the host. Humans can acquire T. spiralis infection only if they consume undercooked or raw meat containing muscle larvae [36]. Several genes are differentially expressed among the life cycle stages and up-regulated genes in the newborn larvae. The genome of T. spiralis is regulated in the developmental stages [34].
8. Future perspective
Trichinella infection is an emerging zoonosis in many countries and where it become the reason for trichinellosis disease. Due to its widespread prevalence and high amount of pork meat consumption more clinical awareness is required. The acute infection is characterized by two phases viz. enteral which disrupt intestinal functions and parenteral phases are associated with the inflammatory and allergic reaction. The diagnosis of this disease contains new specific serological tests such as immunoblot or ELISA. Anthelmintics and anti-inflammatory drugs are the drug of choice for Trichinella infection [16]. Vaccines formulated for veterinary purposes have made a great impact not only on animal welfare, production, and health but also on human health. Vaccines are considered reliable, efficient, and sustainable for the control and prevention of parasitic infection.
In Thrichinellosis, induction of protective and therapeutic responses should evoke both innate and adaptive immune systems to prevent the establishment of parasites in the organism. The life cycle of T. spiralis is complex, and the immune response is not strong that induced by a vaccine containing specific antigen to overcome the challenging infection. Therefore, a vaccine containing multiple epitopes against T. spiralis induces higher immunity [24]. Probiotics such as Lactobacillus keep the environment of the intestine healthy and prevent enteric infections. Probiotic Lactobacillus casei is most commonly used for protection against Trichinellosis. L. casei is involved in the production of IL-4, IgA, and IgG (anti-T. spiralis antibodies) and has a preventive role against high infection of T. spiralis. Some strains of L. casei include L. casei ATCC 469, L. casei ATCC 7469, and L. casei Shirota have proven efficacy against T. spiralis infection. For the control of Trichinellosis, Probiotics and plants-based veterinary vaccines are a new approach and can be used as treatment and edible vaccines for various parasitic diseases in animals. Due to the low cost of plants production, sterile delivery, and transportation at a suitable temperature, plants are considered as a suitable vehicle for veterinary vaccines [1].
Antigens in the vaccines administered orally are subject to proteolysis by the proteolytic enzymes present in the digestive tract of the organism. It will decrease the bioavailability of the vaccines and will induce a low immune response [1]. On the other hand, in plant-based vaccines antigens are protected from proteolytic enzymes by the cell wall of the plant cells and enable antigens to reach their desired destination (gut-associated lymphoid tissue). Various plants and vegetable species such as potato, tomato, tobacco, alfalfa, rice, spinach, beans, maize, strawberries, and carrots can be used in the biotechnology of plants for the expression and production of recombinant proteins.
For the prevention and control of diseases in animals and their transmission from animals to humans, plant-based vaccines seem to be an excellent tool. More research is required to thoroughly understand the applications of medical plant extracts, probiotics, and other biological agents [24].
9. Conclusion
At least twelve species and genotypes of Trichinella genus can cause veterinary or medical health hazards in a wide geographical range throughout the world. The main etiological agent of Trichinellosis in humans is only T. spiralis parasite and can result in mild to severe clinical signs and symptoms. Numerous antigens are used as candidate vaccines from different stages of T. spiralis and can be used as DNA vaccines or recombinant protein vaccines. The role of progesterone and mifepristone against T. spiralis is also very helpful as they penetrate the vaccine into the target of T. spiralis. Altogether, we can get different strains for specific vaccines with molecular physiognomies of different Trichinella species.
Acknowledgments
The author wishes to thank all other co-authors for providing guidance and support.
Conflict of interest
The authors declare that they have no conflict of interest.
\n',keywords:"Trichinella spiralis, immune response, progesterone receptor, hormones, vaccine",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/81718.pdf",chapterXML:"https://mts.intechopen.com/source/xml/81718.xml",downloadPdfUrl:"/chapter/pdf-download/81718",previewPdfUrl:"/chapter/pdf-preview/81718",totalDownloads:14,totalViews:0,totalCrossrefCites:0,dateSubmitted:"December 19th 2021",dateReviewed:"February 2nd 2022",datePrePublished:"May 11th 2022",datePublished:null,dateFinished:"May 11th 2022",readingETA:"0",abstract:"Trichinellosis is a food-borne, zoonotic disease that causes infection by a nematode parasite belonging to the genus Trichinella. This is an important disease, and its causative agent is prevalent throughout the world (cosmopolitan). More clinical awareness of trichinellosis is required due to its many outbreaks, increase in the consumption of pork meat and its by-products. Trichinellosis is an epizootic in nature and its economic burden is associated with the prevention of this disease from the human food chain. This disease is transmitted from animals to humans through the consumption of raw or undercooked meat containing encapsulated muscle larvae of Trichinella spiralis. This paper demonstrates the direct effect of progesterone (P4) and mifepristone (RU486) on the progesterone receptors of T. spiralis. Also, studied the challenges in the preparation of DNA and recombinant protein vaccination to control trichinellosis. It is simply done this study at different life cycle developmental stages of T. spiralis. Vaccines development against T. spiralis infection is the new paradime shift from prevention of trichinellosis to fulfilling the food safety requirements.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/81718",risUrl:"/chapter/ris/81718",signatures:"Muhammad Tahir Aleem, Ruofeng Yan, Asad Khan, Rida Asrar, Amna Shakoor, Areej Asif, Zhaohai Wen, Zhengqing Yu, Muhammad Abdullah Malik, Tauseef-ur-Rehman, Rao Zahid Abbas, Muhammad Mohsin, Xiaokai Song, Lixin Xu and Xiangrui Li",book:{id:"11380",type:"book",title:"Parasitic Helminths and Zoonoses - From Basic to Applied Research",subtitle:null,fullTitle:"Parasitic Helminths and Zoonoses - From Basic to Applied Research",slug:null,publishedDate:null,bookSignature:"Prof. Jorge Morales-Montor, Dr. Víctor Hugo Del Río-Araiza and Dr. Romel Hernández Bello",coverURL:"https://cdn.intechopen.com/books/images_new/11380.jpg",licenceType:"CC BY 3.0",editedByType:null,isbn:"978-1-80355-568-3",printIsbn:"978-1-80355-567-6",pdfIsbn:"978-1-80355-569-0",isAvailableForWebshopOrdering:!0,editors:[{id:"63810",title:"Prof.",name:"Jorge",middleName:null,surname:"Morales-Montor",slug:"jorge-morales-montor",fullName:"Jorge Morales-Montor"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:null,sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Status of anti-T. spiralis vaccine",level:"1"},{id:"sec_3",title:"3. T. spiralis -associated immune mechanism",level:"1"},{id:"sec_4",title:"4. Genomic and proteomic profile of T. spiralis",level:"1"},{id:"sec_4_2",title:"4.1 DNA based vaccine",level:"2"},{id:"sec_5_2",title:"4.2 Protein-based vaccine",level:"2"},{id:"sec_7",title:"5. Role of progesterone receptor in trichinella spiralis",level:"1"},{id:"sec_8",title:"6. Role of progesterone and mifepristone against T. spiralis",level:"1"},{id:"sec_9",title:"7. Challenges to developing an efficient vaccine against T. spiralis",level:"1"},{id:"sec_9_2",title:"7.1 Diversity within T. spiralis parasites",level:"2"},{id:"sec_10_2",title:"7.2 Genetic diversity related to multiple hosts",level:"2"},{id:"sec_11_2",title:"7.3 Multiple stage complexity of T. spiralis",level:"2"},{id:"sec_13",title:"8. Future perspective",level:"1"},{id:"sec_14",title:"9. 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Parasites & Vectors. 2018 Dec;11(1):625. DOI: 10.1186/s13071-018-3198-5'}],footnotes:[],contributors:[{corresp:null,contributorFullName:"Muhammad Tahir Aleem",address:null,affiliation:'
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, China
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, China
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This chapter aims to bring the reader a first-meeting introduction for quickly knowing about MREs, instead of a very deep understanding of MREs.",book:{id:"7685",slug:"smart-and-functional-soft-materials",title:"Smart and Functional Soft Materials",fullTitle:"Smart and Functional Soft Materials"},signatures:"Taixiang Liu and Yangguang Xu",authors:[{id:"283475",title:"Associate Prof.",name:"Yangguang",middleName:null,surname:"Xu",slug:"yangguang-xu",fullName:"Yangguang Xu"}]},{id:"67279",title:"Development, Characterization and Properties of Silk Fibre and Grafted Silk Fibre Reinforced Polymer Composite Films",slug:"development-characterization-and-properties-of-silk-fibre-and-grafted-silk-fibre-reinforced-polymer-",totalDownloads:1107,totalCrossrefCites:1,totalDimensionsCites:1,abstract:"The use of natural fibres over synthetic fibres is gaining widespread importance due to its availability; renewability, low density and satisfactory mechanical properties making them an ecological alternative to synthetic fibres. The innumerable properties of silk fibre have made it superior to be used by researchers both in the plastic and biomedical sector. Silk fibre reinforced PVA (polyvinylalcohol) and PVA/PVP (polyvinyl pyrrolidone) films were prepared via solution casting technique. The effect of silk fibre concentration, on the structural, thermal, mechanical, bio-degradable and the morphological properties of the composite films was assessed. The results indicated that the addition of silk fibres improved the thermal, morphological, mechanical and biodegradable properties of the films. The extensive use of silk fibroin in the biomedical field, due to its robust properties has made it a promising material, suitable in tissue engineering applications. Keeping this in view, the current study also focuses on re-tailoring the properties of silk fibres by grafting a natural polysaccharide like chitosan and thereby fabricate composite films of PVA reinforced with this grafted fibre. The films were tested for their potential applications in tissue engineering, by subjecting them to in vitro biocompatibility tests. The films were also tested for their antibacterial properties. The results thus obtained indicated that the films were non-toxic in all concentrations and were found to be suitable for biomaterial applications.",book:{id:"8162",slug:"generation-development-and-modifications-of-natural-fibers",title:"Generation, Development and Modifications of Natural Fibers",fullTitle:"Generation, Development and Modifications of Natural Fibers"},signatures:"Sareen Sheik and Gundibasappa Karikannar Nagaraja",authors:[{id:"285152",title:"Prof.",name:"G.K.",middleName:null,surname:"Nagaraja",slug:"g.k.-nagaraja",fullName:"G.K. Nagaraja"},{id:"285153",title:"Ms.",name:"Sareen",middleName:null,surname:"Sheik",slug:"sareen-sheik",fullName:"Sareen Sheik"}]},{id:"38395",title:"Structural Health Monitoring for Composite Materials",slug:"structural-health-monitoring-for-composite-materials",totalDownloads:7746,totalCrossrefCites:8,totalDimensionsCites:27,abstract:null,book:{id:"3052",slug:"composites-and-their-applications",title:"Composites and Their Applications",fullTitle:"Composites and Their Applications"},signatures:"Jian Cai, Lei Qiu, Shenfang Yuan, Lihua Shi, PeiPei Liu and Dong Liang",authors:[{id:"140597",title:"Dr.",name:"Jian",middleName:null,surname:"Cai",slug:"jian-cai",fullName:"Jian Cai"},{id:"140715",title:"Prof.",name:"Shenfang",middleName:null,surname:"Yuan",slug:"shenfang-yuan",fullName:"Shenfang Yuan"}]},{id:"69714",title:"Natural Fibers: Applications",slug:"natural-fibers-applications",totalDownloads:1668,totalCrossrefCites:4,totalDimensionsCites:7,abstract:"Fibers derived from bio-based sources such as vegetables and animal origin are termed as natural fibers. This definition includes all natural cellulosic fibers (cotton, jute, sisal, coir, flax, hemp, abaca, ramie, etc.) and protein-based fibers such as wool and silk. There are also man-made cellulose fibers (e.g., viscose rayon and cellulose acetate) that are produced with chemical procedures from pulped wood or other sources (cotton, bamboo). Natural fibers being cost effective and abundantly available yields high potential in various industrial and commercial applications such as in the interior applications of the passenger cars, panels for partition and false ceiling, partition boards, roof tiles, coir fibers in packaging, furniture applications, as insulating materials in low energy houses, geo-textiles for soil protection and erosion control, enhancing barrier properties, composites etc. Due to research and developmental work in modification and treatment methods of natural fibers, utilization of natural fibers has observed a significant growth in various applications. The chapter addresses the potential applications of natural fibers in various commercial sectors for the development of environment-friendly products with an aim to replace synthetic fibers or inorganic fillers with cost-effective and efficient products.",book:{id:"8162",slug:"generation-development-and-modifications-of-natural-fibers",title:"Generation, Development and Modifications of Natural Fibers",fullTitle:"Generation, Development and Modifications of Natural Fibers"},signatures:"Jatinder Singh Dhaliwal",authors:[{id:"272683",title:"Mr.",name:"Jatinder Singh",middleName:null,surname:"Dhaliwal",slug:"jatinder-singh-dhaliwal",fullName:"Jatinder Singh Dhaliwal"}]}],onlineFirstChaptersFilter:{topicId:"934",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:89,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:32,numberOfPublishedChapters:318,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:141,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:106,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:5,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:15,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}}]},series:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"June 29th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:32,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:4,paginationItems:[{id:"14",title:"Cell and Molecular Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",isOpenForSubmission:!0,editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",slug:"rosa-maria-martinez-espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",biography:"Dr. Rosa María Martínez-Espinosa has been a Spanish Full Professor since 2020 (Biochemistry and Molecular Biology) and is currently Vice-President of International Relations and Cooperation development and leader of the research group 'Applied Biochemistry” (University of Alicante, Spain). Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. He performed post-doctoral studies at Max-Planck Institute, Germany, and University of Florence, Italy in addition to making several scientific visits abroad. He currently works as a Full Professor of Biochemistry in the Faculty of Pharmacy, Anadolu University, Turkey. Dr. Beydemir has published over a hundred scientific papers spanning protein biochemistry, enzymology and medicinal chemistry, reviews, book chapters and presented several conferences to scientists worldwide. He has received numerous publication awards from various international scientific councils. He serves in the Editorial Board of several international journals. Dr. Beydemir is also Rector of Bilecik Şeyh Edebali University, Turkey.",institutionString:null,institution:{name:"Anadolu University",institutionURL:null,country:{name:"Turkey"}}},editorTwo:{id:"13652",title:"Prof.",name:"Deniz",middleName:null,surname:"Ekinci",slug:"deniz-ekinci",fullName:"Deniz Ekinci",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYLT1QAO/Profile_Picture_1634557223079",biography:"Dr. Deniz Ekinci obtained a BSc in Chemistry in 2004, MSc in Biochemistry in 2006, and PhD in Biochemistry in 2009 from Atatürk University, Turkey. He studied at Stetson University, USA, in 2007-2008 and at the Max Planck Institute of Molecular Cell Biology and Genetics, Germany, in 2009-2010. Dr. Ekinci currently works as a Full Professor of Biochemistry in the Faculty of Agriculture and is the Head of the Enzyme and Microbial Biotechnology Division, Ondokuz Mayıs University, Turkey. He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. Dr. Ekinci serves as the Editor in Chief of four international books and is involved in the Editorial Board of several international journals.",institutionString:null,institution:{name:"Ondokuz Mayıs University",institutionURL:null,country:{name:"Turkey"}}},editorThree:null},{id:"17",title:"Metabolism",coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",isOpenForSubmission:!0,editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",slug:"yannis-karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",biography:"Yannis Karamanos, born in Greece in 1953, completed his pre-graduate studies at the Université Pierre et Marie Curie, Paris, then his Masters and Doctoral degree at the Université de Lille (1983). He was associate professor at the University of Limoges (1987) before becoming full professor of biochemistry at the Université d’Artois (1996). He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. His teaching areas are energy metabolism and regulation, integration and organ specialization and metabolic adaptation.",institutionString:null,institution:{name:"Artois University",institutionURL:null,country:{name:"France"}}},editorTwo:null,editorThree:null},{id:"18",title:"Proteomics",coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",isOpenForSubmission:!0,editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",slug:"paolo-iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",biography:"Paolo Iadarola graduated with a degree in Chemistry from the University of Pavia (Italy) in July 1972. He then worked as an Assistant Professor at the Faculty of Science of the same University until 1984. In 1985, Prof. Iadarola became Associate Professor at the Department of Biology and Biotechnologies of the University of Pavia and retired in October 2017. Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. He is a Consultant Reviewer for several journals, including the Journal of Chromatography A, Journal of Chromatography B, Plos ONE, Proteomes, International Journal of Molecular Science, Biotech, Electrophoresis, and others. He is also Associate Editor of Biotech.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",slug:"simona-viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",biography:"Simona Viglio is an Associate Professor of Biochemistry at the Department of Molecular Medicine at the University of Pavia. She has been working since 1995 on the determination of proteolytic enzymes involved in the degradation process of connective tissue matrix and on the identification of biological markers of lung diseases. She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. She is an author of about 90 publications (According to Scopus: H-Index: 23; According to WOS: H-Index: 20) on peer-reviewed journals, a member of the “Società Italiana di Biochimica e Biologia Molecolare,“ and a Consultant Reviewer for International Journal of Molecular Science, Journal of Chromatography A, COPD, Plos ONE and Nutritional Neuroscience.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null}]},overviewPageOFChapters:{paginationCount:36,paginationItems:[{id:"82195",title:"Endoplasmic Reticulum: A Hub in Lipid Homeostasis",doi:"10.5772/intechopen.105450",signatures:"Raúl Ventura and María Isabel Hernández-Alvarez",slug:"endoplasmic-reticulum-a-hub-in-lipid-homeostasis",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Endoplasmic Reticulum",coverURL:"https://cdn.intechopen.com/books/images_new/11674.jpg",subseries:{id:"14",title:"Cell and Molecular Biology"}}},{id:"82409",title:"Purinergic Signaling in Covid-19 Disease",doi:"10.5772/intechopen.105008",signatures:"Hailian Shen",slug:"purinergic-signaling-in-covid-19-disease",totalDownloads:5,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Purinergic System",coverURL:"https://cdn.intechopen.com/books/images_new/10801.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"82374",title:"The Potential of the Purinergic System as a Therapeutic Target of Natural Compounds in Cutaneous Melanoma",doi:"10.5772/intechopen.105457",signatures:"Gilnei Bruno da Silva, Daiane Manica, Marcelo Moreno and Margarete Dulce Bagatini",slug:"the-potential-of-the-purinergic-system-as-a-therapeutic-target-of-natural-compounds-in-cutaneous-mel",totalDownloads:10,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Purinergic System",coverURL:"https://cdn.intechopen.com/books/images_new/10801.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"82103",title:"The Role of Endoplasmic Reticulum Stress and Its Regulation in the Progression of Neurological and Infectious Diseases",doi:"10.5772/intechopen.105543",signatures:"Mary Dover, Michael Kishek, Miranda Eddins, Naneeta Desar, Ketema Paul and Milan Fiala",slug:"the-role-of-endoplasmic-reticulum-stress-and-its-regulation-in-the-progression-of-neurological-and-i",totalDownloads:6,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Endoplasmic Reticulum",coverURL:"https://cdn.intechopen.com/books/images_new/11674.jpg",subseries:{id:"14",title:"Cell and Molecular Biology"}}}]},overviewPagePublishedBooks:{paginationCount:32,paginationItems:[{type:"book",id:"7006",title:"Biochemistry and Health Benefits of Fatty Acids",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7006.jpg",slug:"biochemistry-and-health-benefits-of-fatty-acids",publishedDate:"December 19th 2018",editedByType:"Edited by",bookSignature:"Viduranga Waisundara",hash:"c93a00abd68b5eba67e5e719f67fd20b",volumeInSeries:1,fullTitle:"Biochemistry and Health Benefits of Fatty Acids",editors:[{id:"194281",title:"Dr.",name:"Viduranga Y.",middleName:null,surname:"Waisundara",slug:"viduranga-y.-waisundara",fullName:"Viduranga Y. Waisundara",profilePictureURL:"https://mts.intechopen.com/storage/users/194281/images/system/194281.jpg",biography:"Dr. Viduranga Waisundara obtained her Ph.D. in Food Science\nand Technology from the Department of Chemistry, National\nUniversity of Singapore, in 2010. She was a lecturer at Temasek Polytechnic, Singapore from July 2009 to March 2013.\nShe relocated to her motherland of Sri Lanka and spearheaded the Functional Food Product Development Project at the\nNational Institute of Fundamental Studies from April 2013 to\nOctober 2016. She was a senior lecturer on a temporary basis at the Department of\nFood Technology, Faculty of Technology, Rajarata University of Sri Lanka. She is\ncurrently Deputy Principal of the Australian College of Business and Technology –\nKandy Campus, Sri Lanka. She is also the Global Harmonization Initiative (GHI)",institutionString:"Australian College of Business & Technology",institution:null}]},{type:"book",id:"6820",title:"Keratin",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/6820.jpg",slug:"keratin",publishedDate:"December 19th 2018",editedByType:"Edited by",bookSignature:"Miroslav Blumenberg",hash:"6def75cd4b6b5324a02b6dc0359896d0",volumeInSeries:2,fullTitle:"Keratin",editors:[{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. 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Dr. Şentürk currently works as an professor of Biochemistry in the Department of Basic Pharmacy Sciences, Faculty of Pharmacy, Ağri Ibrahim Cecen University, Turkey. \nDr. Şentürk published over 120 scientific papers, reviews, and book chapters and presented several conferences to scientists. \nHis research interests span enzyme inhibitor or activator, protein expression, purification and characterization, drug design and synthesis, toxicology, and pharmacology. \nHis research work has focused on neurodegenerative diseases and cancer treatment. 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He then worked as an Assistant Professor at the Faculty of Science of the same University until 1984. In 1985, Prof. Iadarola became Associate Professor at the Department of Biology and Biotechnologies of the University of Pavia and retired in October 2017. Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. 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She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. She is an author of about 90 publications (According to Scopus: H-Index: 23; According to WOS: H-Index: 20) on peer-reviewed journals, a member of the “Società Italiana di Biochimica e Biologia Molecolare,“ and a Consultant Reviewer for International Journal of Molecular Science, Journal of Chromatography A, COPD, Plos ONE and Nutritional Neuroscience.",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"2",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null,series:{id:"11",title:"Biochemistry"}}},seriesLanding:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"June 29th, 2022",hasOnlineFirst:!0,numberOfOpenTopics:4,numberOfPublishedChapters:318,numberOfPublishedBooks:32,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},subseries:[{id:"14",title:"Cell and Molecular Biology",keywords:"Omics (Transcriptomics; Proteomics; Metabolomics), Molecular Biology, Cell Biology, Signal Transduction and Regulation, Cell Growth and Differentiation, Apoptosis, Necroptosis, Ferroptosis, Autophagy, Cell Cycle, Macromolecules and Complexes, Gene Expression",scope:"The Cell and Molecular Biology topic within the IntechOpen Biochemistry Series aims to rapidly publish contributions on all aspects of cell and molecular biology, including aspects related to biochemical and genetic research (not only in humans but all living beings). We encourage the submission of manuscripts that provide novel and mechanistic insights that report significant advances in the fields. Topics include, but are not limited to: Advanced techniques of cellular and molecular biology (Molecular methodologies, imaging techniques, and bioinformatics); Biological activities at the molecular level; Biological processes of cell functions, cell division, senescence, maintenance, and cell death; Biomolecules interactions; Cancer; Cell biology; Chemical biology; Computational biology; Cytochemistry; Developmental biology; Disease mechanisms and therapeutics; DNA, and RNA metabolism; Gene functions, genetics, and genomics; Genetics; Immunology; Medical microbiology; Molecular biology; Molecular genetics; Molecular processes of cell and organelle dynamics; Neuroscience; Protein biosynthesis, degradation, and functions; Regulation of molecular interactions in a cell; Signalling networks and system biology; Structural biology; Virology and microbiology.",annualVolume:11410,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"79367",title:"Dr.",name:"Ana Isabel",middleName:null,surname:"Flores",fullName:"Ana Isabel Flores",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRpIOQA0/Profile_Picture_1632418099564",institutionString:null,institution:{name:"Hospital Universitario 12 De Octubre",institutionURL:null,country:{name:"Spain"}}},{id:"328234",title:"Ph.D.",name:"Christian",middleName:null,surname:"Palavecino",fullName:"Christian Palavecino",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000030DhEhQAK/Profile_Picture_1628835318625",institutionString:null,institution:{name:"Central University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"186585",title:"Dr.",name:"Francisco Javier",middleName:null,surname:"Martin-Romero",fullName:"Francisco Javier Martin-Romero",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB3HQAW/Profile_Picture_1631258137641",institutionString:null,institution:{name:"University of Extremadura",institutionURL:null,country:{name:"Spain"}}}]},{id:"15",title:"Chemical Biology",keywords:"Phenolic Compounds, Essential Oils, Modification of Biomolecules, Glycobiology, Combinatorial Chemistry, Therapeutic peptides, Enzyme Inhibitors",scope:"Chemical biology spans the fields of chemistry and biology involving the application of biological and chemical molecules and techniques. In recent years, the application of chemistry to biological molecules has gained significant interest in medicinal and pharmacological studies. This topic will be devoted to understanding the interplay between biomolecules and chemical compounds, their structure and function, and their potential applications in related fields. Being a part of the biochemistry discipline, the ideas and concepts that have emerged from Chemical Biology have affected other related areas. 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Behind these definitions are hidden all the aspects of normal and pathological functioning of all processes that the topic ‘Metabolism’ will cover within the Biochemistry Series. Thus all studies on metabolism will be considered for publication.",annualVolume:11413,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",institutionString:null,institution:{name:"Artois University",institutionURL:null,country:{name:"France"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"243049",title:"Dr.",name:"Anca",middleName:null,surname:"Pantea Stoian",fullName:"Anca Pantea Stoian",profilePictureURL:"https://mts.intechopen.com/storage/users/243049/images/system/243049.jpg",institutionString:null,institution:{name:"Carol Davila University of Medicine and Pharmacy",institutionURL:null,country:{name:"Romania"}}},{id:"203824",title:"Dr.",name:"Attilio",middleName:null,surname:"Rigotti",fullName:"Attilio Rigotti",profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institutionString:null,institution:{name:"Pontifical Catholic University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"300470",title:"Dr.",name:"Yanfei (Jacob)",middleName:null,surname:"Qi",fullName:"Yanfei (Jacob) Qi",profilePictureURL:"https://mts.intechopen.com/storage/users/300470/images/system/300470.jpg",institutionString:null,institution:{name:"Centenary Institute of Cancer Medicine and Cell Biology",institutionURL:null,country:{name:"Australia"}}}]},{id:"18",title:"Proteomics",keywords:"Mono- and Two-Dimensional Gel Electrophoresis (1-and 2-DE), Liquid Chromatography (LC), Mass Spectrometry/Tandem Mass Spectrometry (MS; MS/MS), Proteins",scope:"With the recognition that the human genome cannot provide answers to the etiology of a disorder, changes in the proteins expressed by a genome became a focus in research. Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. Currently, proteomics relies mainly on mass spectrometry (MS) combined with electrophoretic (1 or 2-DE-MS) and/or chromatographic techniques (LC-MS/MS). MS is an excellent tool that has gained popularity in proteomics because of its ability to gather a complex body of information such as cataloging protein expression, identifying protein modification sites, and defining protein interactions. The Proteomics topic aims to attract contributions on all aspects of MS-based proteomics that, by pushing the boundaries of MS capabilities, may address biological problems that have not been resolved yet.",annualVolume:11414,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null,editorialBoard:[{id:"72288",title:"Dr.",name:"Arli Aditya",middleName:null,surname:"Parikesit",fullName:"Arli Aditya Parikesit",profilePictureURL:"https://mts.intechopen.com/storage/users/72288/images/system/72288.jpg",institutionString:null,institution:{name:"Indonesia International Institute for Life Sciences",institutionURL:null,country:{name:"Indonesia"}}},{id:"40928",title:"Dr.",name:"Cesar",middleName:null,surname:"Lopez-Camarillo",fullName:"Cesar Lopez-Camarillo",profilePictureURL:"https://mts.intechopen.com/storage/users/40928/images/3884_n.png",institutionString:null,institution:{name:"Universidad Autónoma de la Ciudad de México",institutionURL:null,country:{name:"Mexico"}}},{id:"81926",title:"Dr.",name:"Shymaa",middleName:null,surname:"Enany",fullName:"Shymaa Enany",profilePictureURL:"https://mts.intechopen.com/storage/users/81926/images/system/81926.png",institutionString:"Suez Canal University",institution:{name:"Suez Canal University",institutionURL:null,country:{name:"Egypt"}}}]}]}},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"chapter.detail",path:"/chapters/41896",hash:"",query:{},params:{id:"41896"},fullPath:"/chapters/41896",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()