Macrophages and neutrophils are the sentinel cells of the innate immune response of vertebrates, such as bony fish (teleosts). As phagocytic myeloid cells, they are involved in homeostatic mechanisms, wound healing, and the detection, elimination and clearance of foreign entities including tumors, virus-infected cells and invading pathogens. Furthermore, macrophages and neutrophils are responsible for producing hundreds of bioactive molecules that are important in pathogen recognition and destruction, cellular communication and activation, initiation of an adaptive immune response and later, resolution of an inflammatory response and tissue repair. Neutrophils and macrophages, while essential to survival, have a finite lifespan. Therefore, a manufacturing centre, the hematopoietic niche, is needed for the production of myeloid cells. The hematopoietic niche must maintain basal myeloid cell production levels during homeostasis, yet retain the flexibility to ramp-up cell production in response to physiological demands, such as pathogenic insult. The development of macrophages (monopoiesis) and neutrophils (granulopoiesis) is collectively known as myelopoiesis, and is regulated by the complex interaction of colony-stimulating factors (CSFs), their receptors, and intracellular transcription factor machinery that control lineage fate decisions and terminal differentiation events.
Over the past 50 years, research using the mouse model system has culminated in the identification of the site(s) of myelopoiesis, the progenitor cell types that give rise to mature myeloid cells, the extracellular and intracellular cues required, and a detailed understanding of the complex intracellular and extracellular milieu of factors that drive this tightly controlled process. From these studies we understand hematopoiesis as an exquisitely fine-tuned, highly regulated, process whereby all blood cells develop from a small number of hematopoietic stem cells (HSCs). HSCs are characterized as long-term repopulating, pluripotent, quiescent cells that undergo symmetrical self-renewal to sustain the population of HSCs within the hematopoietic niche, or asymmetrical division to give rise to hematopoietic progenitor cells (HPCs) . HPCs can develop along the lymphoid lineage, termed lymphopoiesis, to give rise to B-cells, T-cells, natural killer (NK) cells and dendritic cells (DCs). Alternatively, HPCs can develop along an erythroid lineage, termed erythropoiesis, to give rise to erythrocytes and megakaryocytes, or develop along a myeloid lineage to give rise to granulocytes (neutrophils, basophils, eosinophils, mast cells), mononuclear phagocytes (monocytes and macrophages), and DCs. The lymphoid lineage represents the adaptive arm of the immune response, while the myeloid lineage represents the innate arm of the immune response. Regardless of lineage, the decisions made to commit and develop along a given lineage are controlled by extracellular growth factors and intracellular transcription factors that act in concert to regulate gene and protein expression to achieve the desired outcome.
When compared to the mechanisms of myelopoiesis in the mouse, studies using lower vertebrates, such as teleosts, have identified both evolutionary conservation as well as divergence in the mechanisms of myelopoiesis. With over 30,000 identified species, teleosts are the most expansive class of vertebrates, and represent an excellent model system to study the evolution of vertebrate myelopoiesis as they are one of the ancient classes of vertebrates to retain the production of myeloid cells. Within the teleost system, much research surrounds the characterization of teleost cytokines and receptors involved in inflammation and their cellular targets (primarily macrophages). In comparison, little is known about the mechanisms that govern myeloid cell production. Research on teleost myelopoiesis is hampered by the lack of reagents, the difficulty in isolating appreciable numbers of relatively pure populations of HSCs/HPCs, and in identifying key growth factors important for myeloid cell development due to evolutionary selection pressures. As such, the focus of this review is to provide an overview of the current knowledge of the fish model systems used and the growth factors, receptors and transcription factors involved in teleost myelopoiesis, using information from the mammalian model systems as a scaffold to put the advances into context.
2. Teleost model systems of myelopoiesis
2.1. Zebrafish model system
The zebrafish model has been instrumental in advancing our knowledge of the sites of hematopoiesis/myelopoiesis in teleosts, the development, differentiation and migration of HSCs, and through genetic manipulation, the characterization of the early acting growth factors, receptors and transcription factors involved in hematopoiesis. By far, the major advantage provided by zebrafish is the ease of generating transgenic zebrafish, morphant zebrafish (morpholinos) and knockout zebrafish (zinc finger nucleases), as well as many others, due to genetic manipulation. In conjunction, the rapid generation of embryos, embryonic transparency, and small embryo size allows for mass screening strategies. The advantages of zebrafish as a model system, and their contributions to hematopoiesis have been extensively reviewed elsewhere [2-5], and thus will not be covered in this review. While the zebrafish is an excellent
2.2. Ginbuna crucian carp model system
The ginbuna crucian carp model system has been instrumental in serving as a close parallel to the mouse model system in terms of hematopoietic reconstitution experiments to demonstrate the existence of HSCs in teleosts. Identification of donor and recipient HSCs/HPCs and characterization of their progeny by ploidy analysis is useful for assessing the multipotency of different progenitor cell populations. Furthermore, the use of this model system has allowed for the determination of the location of HSCs within the hematopoietic organs of cyprinids. Use of the ginbuan crucian carp system will be particularly important for future work as antibodies are developed against markers on the surface of fish HSCs and HPCs to allow for the analysis of the potency of progenitor cell subpopulations.
2.3. Goldfish model system
The goldfish model system represents a unique opportunity to study myelopoiesis
The spontaneous proliferation and differentiation of PKMs suggested the production of endogenous growth factors and prompted the examination of the target cell sub-population(s) upon which they acted and their effects on cell proliferation and differentiation. The putative progenitors (R1 cells) and macrophages (R2 cells), but not monocytes, were determined to be responsible for the production of endogenous growth factors that act in an autocrine and paracrine fashion . Addition of cell-conditioned medium (CCM) to sorted cell populations demonstrated the capacity of putative progenitors and monocytes to proliferate and differentiate in response to endogenous growth factors. However, treatment of macrophages (R2 cells) with CCM demonstrated their apparent terminal differentiation, while their capacity to proliferate suggested they were capable of self-renewal [15, 17]. Clearly, different endogenous growth factors present in CCM exert distinct actions on macrophage cell sub-populations.
Two pathways of macrophage development were proposed to occur in the PKM cultures. The predominant pathway was classical macrophage development in which progenitor cells differentiated into monocytes and then macrophages . The second was an alternative pathway of macrophage development in which progenitor cells differentiated into macrophages without a prominent monocytic stage . The possible retention of the alternative pathway of macrophage production in addition to the classical pathway may provide a mechanism for rapid generation of macrophages during injury or infection
The observed kinetics of the PKM cultures suggested three phases of growth. Initially, there is a lag phase (days 1-4) where many cells die, followed by a proliferative phase (days 5-9) where cell numbers rapidly increase , and finally, a senescence phase (days 10-14) characterized by cell clumping and cell apoptosis [17, 18]. Differential cross screening of proliferative versus senescence phase PKMs identified a number of differentially expressed genes including those involved in hematopoiesis, signal transduction, transcription, translation and protein processing . The involvement of the identified transcripts in the regulation of cell development [20-22] will be discussed in the following sections.
These seminal observations from PKM cultures established three important ideas regarding goldfish monopoiesis: (1) kidney leukocytes produce their own endogenous growth factors important for driving proliferation and differentiation [14, 15]. (2) Within the population of small leukocyte R1 cells, a population of macrophage progenitor cells must exist. (3) Unlike mammalian systems, the progenitor cell population gives rise to fully differentiated macrophages
3. Site of hematopoiesis/myelopoiesis
3.1. Two waves of hematopoiesis in vertebrates
There are two waves of hematopoiesis in vertebrates. The first wave is primitive hematopoiesis and occurs during embryonic development. Definitive hematopoiesis follows primitive hematopoiesis and occurs in the post-natal or adult animal. Primitive and definitive hematopoiesis are different on a temporal scale, a spatial scale, and in the types of cellular progeny generated. With the exception of T-cells, that undergo maturation in the thymus, lymphopoiesis and myelopoiesis occur in the major hematopoietic organs. The major hematopoietic organ of teleosts is the kidney, akin to that of mammalian bone marrow.
3.2. Primitive myelopoiesis in teleosts
The development of myelopoiesis in fish has primarily been studied using the zebrafish model system. Primitive myelopoiesis is predominated by HPCs with primarily erythroid and myeloid development potential. Initially, primitive hematopoiesis is initiated in the anterior lateral mesoderm (ALM), that gives rise to the rostral blood island (RBI), and in the posterior lateral mesoderm (PLM), that gives rise to the intermediate cell mass (ICM). The RBI is the site of primitive myeloid cell development, generating primarily primitive macrophages that undergo rapid differentiation, lacking or having a very short monocytic stage  and a few neutrophils , while the ICM is the site of primitive erythroid cell development . This stage of primitive hematopoiesis occurs early during development of zebrafish, approximately 11 hours post fertilization (hpf). Following the onset of circulation, at around 24 hpf, the site of hematopoiesis then switches to the posterior blood island (PBI)  and produces multi-lineage progenitor cells capable of producing both primitive erythroid and myeloid cells . Primitive macrophages act as phagocytes during tissue remodeling throughout embryonic development and in clearance of bacterial pathogens . While primitive neutrophils also migrate to a site of infection, they were not observed to phagocytose bacteria . The temporal, spatial and transcriptional control of zebrafish primitive hematopoiesis has been reviewed by [28-30]. Differences in the initial site of hematopoiesis occur between fish species, however, the production of erythrocytes and macrophages during primitive hematopoiesis is consistent [31, 32].
3.3. Definitive myelopoiesis in teleosts
The onset of definitive myelopoiesis occurs around 36 hpf in the zebrafish. Here, HSCs seed the aorta-gonad-mesonephros (AGM) and the caudal hematopoietic tissue (CHT) [33, 34]. By 48 hpf, the HSCs seed the kidney , the final hematopoietic site equivalent to mammalian bone marrow [35-37].
The existence of teleost kidney HSCs and HPCs capable of generating all hematopoietic lineages was demonstrated using transplantation studies in zebrafish and ginbuna crucian carp. Transplantation of whole kidney marrow from
4. Commitment to the myeloid lineage
4.1. Progression of cell development
From the mouse model we know that the commitment of a pluripotent, self-renewing HSC to a common myeloid progenitor (CMP) is a progression of lineage fate decisions controlled by extracellular cues, such as growth factors, within the hematopoietic niche [46-48], as well as the modulation of intracellular transcription factors [49-52]. The process of committing to a CMP begins with long-term HSCs (LT-HSCs), capable of self-renewal and multi-lineage differentiation. LT-HSCs give rise to short-term HSCs (ST-HSCs) with limited capacity for self-renewal, which then differentiate into multipotent progenitors (MPPs) with no ability to self-renew, reviewed by . The MPPs can give rise to the CMP or the lymphoid-myeloid primed multipotent progenitors (LMPPs) [54-57]. The CMP can differentiate into megakaryocyte/erythroid progenitor (MEP) or to a granulocyte/macrophage progenitor (GMP)  (Figure 1). The LMPPs can differentiate into a common lymphoid precursor (CLP) that gives rise to T- and B-lymphocytes, or can also give rise to GMPs [54-57, 59, 60], and reviewed in .
On the other hand, the “myeloid-based model” of hematopoiesis, in which myeloid potential is retained in erythroid, T, and B cell branches even after these lineages have segregated from each other, has been proposed . Notably, there is no CLP in this model [63-65]. According to this model, hematopoiesis can be understood as follows: specification toward erythroid, T, and B cell lineages proceeds on a basis of a prototypical developmental program to construct myeloid cells [66, 67]. Indeed, several findings in teleosts are supportive of the myeloid-based model [68, 69]. In the future, the myeloid-based model may bring a paradigm shift in the concept of blood cell lineage development. In the following sections the key growth factors and transcription factors studied in the teleost system will be discussed.
4.2. Receptors and growth factors
4.2.1. Mammalian stem cell factor and Kit receptor
Stem cell factor (SCF) was identified [70-72] as short-chain four-helix bundle  encoded by the
The SCF receptor, c-KIT (CD117), was first identified as the cellular oncogene (
The c-KIT protein is primarily found on hematopoietic cells and is a marker of long-term reconstituting HSCs in humans  and mice [90-92]. c-KIT is expressed on pluripotent and multipotent HSCs and myeloerythroid precursors, but not on differentiating or mature cell types [90-92], with the exception of mast cells . Approximately 2 x 104 c-KIT receptors are found on normal human HPCs , and can undergo proteolytic cleavage to release a soluble form of c-KIT [95-97]. The soluble c-KIT receptor is thought to regulate membrane bound c-KIT activity,
Binding of homodimeric SCF to c-KIT results in receptor homodimerization, conformational changes in the extracellular and intracellular domains and autophosphorylation of the intracellular tyrosines (reviewed extensively in [73, 78, 99-105]) leading to a number of down-stream signaling pathways that mediate the action of SCF through c-KIT. These signaling pathways include phosphatidylinositol-3-kinase (PI3K), phospholipase Cγ (PLCγ), members of the Janus family of protein tyrosine kinases (JAK) and signal transducers and activators of transcription (STATs), Src family members, the Ras/Raf/MAP kinase pathway, and others. The signaling pathway initiated depends on the cell type, and the strength and duration of the signal, reviewed in [106-108].
4.2.2. Biological functions of stem cell factor
SCF and its type III tyrosine kinase receptor c-KIT, are involved in hematopoiesis [81, 107, 108], spermatogenesis [109-111], and development of melanocytes [110, 112-114] and mast cells [93, 96, 115-120]. Within the hematopoietic niche, one role of SCF/c-KIT is to mediate HSC and HPC survival, important for the generation of spleen, interleukin-3 (IL-3), granulocyte/macrophage, and macrophage colony-forming units (CFU-S, CFU-IL-3, CFU-GM, and CFU-M) . Further studies have confirmed SCF/c-KIT to mediate the survival of long-term HSCs by blocking cell cycling or by inhibiting apoptosis [122, 123]. Furthermore, SCF can synergizing with other growth factors, such as granulocyte-macrophage colony-stimulating factor (GM-CSF) , granulocyte colony-stimulating factor (G-CSF), IL-1, IL-3 , IL-6, and IL-7, among others, to promote the proliferation and differentiation of HPCs [125, 126] and reviewed in . Often, the progeny of HPC differentiation depends on the particular growth factor and SCF. Lastly, SCF acts as a homing signal to HPCs, such as CFU-GEMM (granulocyte-erythrocyte-macrophage-megakaryocyte), CFU-GM, CFU-Meg (megakaryocyte) and burst forming units-erythrocyte (BFU-E) 
4.2.3. Teleost Kit and Kit ligand
Whole genome duplication has resulted in two orthologues of
4.2.4. Biological functions of teleost kit ligands and receptors
Based on the non-overlapping expression of
The role of teleost
Lastly, c-KIT plays a role in the development of primordial germ cells (PGCs) in mice. Examination of primordial germ cell development in fish revealed that
4.2.5. Interleukin-3 and Interleukin-3 receptor
Interleukin-3 (IL-3) is a multi-lineage colony-stimulating factor (multi-CSF) that acts through the IL-3 receptor alpha and common beta chain on multipotent erythro/myeloid HPCs to promote their self renewal, proliferation and differentiation [138-140]. IL-3 can also act on committed myeloid progenitors to promote their proliferation and differentiation [138-142]. Interestingly,
4.2.6. Granulocyte-macrophage colony-stimulating factor/Granulocyte-macrophage colony-stimulating factor receptor
GM-CSF shares redundancy with IL-3 in terms of its function. However, GM-CSF acts on a more mature population of HPCs and has been associated with the formation of both granulocyte and macrophage colonies from CFU-GM [147, 148]. GM-CSF is produced by activated T-lymphocytes [147, 149], endothelial cells , and lung fibroblasts  and suggests the importance of GM-CSF during emergency hematopoiesis. GM-CSF promotes the survival, proliferation and differentiation of GMPs [147, 148, 152]. Furthermore, GM-CSF is chemoattractive to immature and mature neutrophils
Similar to that of
4.3. Transcription factors
Commitment of LT-HSCs to the myeloid lineage is an intricate regulation of the transcription factors expressed, their relative levels to one another, and their expression on a temporal scale. Transcription factors (TFs) can act antagonistically or co-operatively. Thus, the presence or absence of a TF partner, or the relative levels of a TF to its antagonistic counterpart, determine lineage fate decisions. Furthermore, the expression of a transcription factor in an HSC does not exert the same effect as when it is expressed in a committed progenitor cell. The transcriptional regulation of mammalian hematopoiesis/myelopoiesis has been extensively reviewed elsewhere [159-162], and will only be briefly described here for the purpose of putting advances in the teleost model systems into context. A visual representation of which stages these transcription factors are important is shown in Figure 2.
In zebrafish, the
CCAAT/enhancer binding proteins (C/EBPs) are members of the family of transcription factors that contain a C-terminal basic leucine zipper domain (bZIP) comprised of a basic region involved in DNA binding and a leucine zipper domain involved in protein interactions . Six members of the C/EBP family have been identified in mammals: alpha, beta, gamma, delta, epsilon and zeta . Orthologues of the C/EBP family of transcription factors have been identified in teleosts [168-171], corresponding to C/EBPα, C/EBPβ, C/EBPγ, C/EBPε, and C/EBPδ.
Expressed in HSCs, CMPs and GMPs [172, 173], C/EBPα has been shown to be involved in directing granulocyte cell fate and terminal differentiation of neutrophils, along with C/EBPε. Mice deficient in C/EBPα show diminished numbers of CFU-GM, CFU-M, CFU-G, macrophages and neutrophils [174, 175]. The loss of myeloid cells in C/EBPα deficient mice is reflective of the role that CEBPα plays in determining the fate of a CMP to a GMP lineage versus an MEP lineage . C/EBPα is capable of binding to the
The zebrafish CEBPα orthologue showed 66% amino acid identity to human C/EBPα, while the bZIP domains showed 99% amino acid identity . In zebrafish,
Two studies have examined the function of CEBPα in zebrafish primitive myelopoiesis. The injection of a deletion mutant of
The orthologues of C/EBPδ, C/EBPγ and C/EBPε exist in teleosts. The
The Ets transcription family member PU.1 is well known as the master transcriptional regulator of mammalian myelopoiesis through an antagonistic relationship with GATA1, recently reviewed by . At the N-terminus, PU.1 comprises of an acidic domain and a glutamine rich domain that are involved in activation of transcription, and a PEST domain important for protein interactions . At the C-terminus, PU.1 has an Ets domain important for binding the DNA consensus sequence AAAG(A/C/G)GGAAG . Mice deficient in PU.1 (
PU.1 also plays a role at the GMP stage to regulate commitment to a granulocyte or macrophage lineage. Increased levels of PU.1 at the GMP stage, along with AP-1 association, drives a monocyte cell fate, while lower levels of PU.1 drives granulocyte cell fate [175, 177]. Furthermore, PU.1 induces
An orthologue of PU.1 has been identified in teleosts. In the Japanese flounder,
A pu.1-like gene (spi-1 like,
5. Commitment of bi-potent myeloid progenitors to the macrophage or neutrophil lineage
5.1. Macrophage development
5.1.1. Progression of cell development
In mammalian systems, the progression of macrophage development proceeds from a committed macrophage progenitor, monoblast, promonocyte, monocyte and then to a mature tissue macrophage, reviewed by [205-207] (Figure 1). While the presence of a unipotent committed macrophage progenitor has yet to be unequivocally demonstrated in the teleost systems, progenitor/precursor cells that give rise to monocytes and macrophages have been demonstrated.
5.1.2. Receptors and growth factors
18.104.22.168. Colony-stimulating factor-1
The central growth factor that regulates the survival, proliferation, and differentiation of macrophages and their precursors is colony-stimulating factor-1 (CSF-1) [220-223]. Alternative splicing of
Recently, IL-34 was identified as another growth factor involved in mediating macrophage development in mammals, in addition to CSF-1 [232-234]. The IL-34 protein does not show homology to any other human protein and or contain any known conserved structural motifs . Homodimeric IL-34 binds to CSF-1R, although with a different affinity than that of CSF-1, and to different sites on the receptor [232, 233] . The hierarchy in binding of the CSF-1R ligands may provide a mechanism for differential signaling depending on the bound ligand. To date, IL-34 has not been identified in teleosts.
22.214.171.124. Colony-stimulating factor-1 receptor
126.96.36.199. Biological functions of colony stimulating factor-1
In addition to the regulation of survival, proliferation, and differentiation of macrophages and their precursors [220-223], CSF-1 has been shown to exert pro-inflammatory effects on monocytes and macrophages. These effects include the enhancement of macrophage chemotaxis, phagocytosis of pathogens, and the production of antimicrobial agents, reviewed by [162, 238]. CSF-1 is a pleiotropic cytokine and functions in a number of other biological systems such as regulation of macrophage and osteoclast numbers, bone remodeling, tooth production and fertility and breast development [241-245]
188.8.131.52. Teleost colony stimulating factor-1
The genomic structure of the identified
Along with differing genomic organizations, trout
184.108.40.206. Teleost colony-stimulating factor-1 receptor
The duplication of
CSF-1R was also identified in goldfish as a 975 aa integral membrane bound protein (mCSF-1R) that possessed the five Ig extracellular domains with multiple N-linked glycosylation sites, a transmembrane domain, and an intracellular tyrosine kinase domain . The mRNA of mCSF-1R could be detected in progenitor, monocyte and macrophage subpopulations, and an antibody produced against the first two Ig domains of CSF-1R was able to recognize monocytes and macrophages . However, unlike mammalian neutrophils, zebrafish and goldfish neutrophils do not appear to express mRNA for
5.2. Neutrophil development
5.2.1. Progression of cell development
Following the commitment of the CFU-GM to a committed granulocyte progenitor cell, terminal differentiation through a promyelocyte, myelocyte, and metamyelocyte stages occur to give rise to a mature neutrophil, and are regulated through growth factor and transcription factor signaling, reviewed by  (Figure 1). Similar to that of mammals, the differentiation of fish neutrophils appears to occur through various stages, based on morphological and cytochemical characteristics, and include the promyelocyte, myelocyte, metamyelocyte and the mature neutrophil, which sometimes had a segmented nucleus [45, 212, 213, 215, 260]. These neutrophils were shown to migrate from the hematopoietic organ to the site of wounding, pathogen injection, or transformed cell injection [24, 45, 261], in response to a hydrogen peroxide attractant produced by cells at the site of damage . However, the responding neutrophils had low phagocytic activity , or engulfed small fragments of the pathogen .
5.2.2. Receptors and growth factors
220.127.116.11. Granulocyte colony-stimulating factor
Neutrophils contribute to both innate and adaptive immune responses. They are capable of chemotaxis, phagocytosis, antimicrobial molecule production, and formation of extracellular traps [262-267]. Upon activation, neutrophils produce a number of chemokines, pro-inflammatory and anti-inflammatory cytokines, as well as the colony-stimulating factors G-CSF, CSF-1, GM-CSF, IL3 and SCF, reviewed by [268, 269]. However, neutrophils are short lived, 6-90 hrs, and need to be continuously replaced.
GCSF, a member of the class I cytokine family, is the primary CSF that mediates the proliferation, differentiation, survival and activation of neutrophils and their progenitors, and has been reviewed extensively by [144, 270]. The transcription of
18.104.22.168. Granulocyte colony-stimulating factor receptor
The protein structure of GCSFR is comprised of a signal peptide, an immunoglobulin-like domain, a cytokine receptor homology (CRH) domain containing the class I cytokine receptor superfamily motif W-S-X-W-S, three fibronectin domains, a transmembrane domain, and an intracellular cytoplasmic signaling domain containing three motifs termed Box 1, Box 2, and Box 3, important for signal transduction [270, 275]. Based on their protein structure and conserved motifs, the human and mouse integral membrane GCSFR proteins were placed in the type I cytokine receptor family.
While there are reports of GCSFR on other hematopoietic cells such as monocytes  and lymphocytes, as well as some non-hematopoietic cells, GCSFR is primarily found on neutrophils and their precursors [270, 277]. Neutrophils up-regulate their levels of GCSFR as they differentiate from progenitor cell to mature neutrophil, with 50-500 GCSF receptors per cell . Structural analysis showed GCSF forms a homodimer, binds two GCSFRs, and leads to receptor homodimerization in a 2:2 complex [279-281]. Binding of a homodimeric GCSF to two GCSF receptors triggers intracellular signaling through the JAK/STAT, Ras/Raf/Erk, or PI3K pathways [275, 277, 282]. These signaling pathways ultimately lead to the migration, survival, proliferation, and differentiation of neutrophils. Control of GCSFR signaling in neutrophils is modulated through (1) transcriptional activation of the
22.214.171.124. Biological activity of granulocyte colony stimulating factor
The targeted gene disruption of
126.96.36.199. Teleost granulocyte colony-stimulating factor
Functional studies on fish GCSF-1 are limited. Only two manuscripts report on the function of GCSF-1 and both utilize the zebrafish model system.
188.8.131.52. Teleost granulocyte colony-stimulating factor receptor
The predicted protein structure of zebrafish and goldfish GCSFRs is conserved across vertebrates. The teleost GCSFR extracellular domain is comprised of a signal peptide, an Ig-like domain, a cytokine homology domain containing the WSXWS motif and four cysteine residues, and three fibronectin domains. Following the transmembrane region, the intracellular region contains predicted Box1, Box2, and Box 3 signaling motifs and 6 tyrosine residues [292, 293], shown to be involved in receptor activation and internalization in higher vertebrates.
5.3. Transcription factors
In addition to the transcription factors described in section 4.3, there are a number of transcription factors downstream that participate in determining GMP fate decisions and that play a role in macrophage and neutrophil cell development, reviewed by [51, 294]. A visual representation of the stage(s) in which these transcription factors are important are shown in Figure 2.
5.3.1. Early growth response (Egr)
The four Egr proteins, EGR1 [295, 296], EGR2 , EGR3  and EGR4 , are members of the zinc finger transcription factor family and have an N-terminus activation domain, a repressor domain capable of binding to NAB1/2, and a DNA binding domain comprised of three zinc fingers that bind to the GC rich sequence, 5’-GCGGGGGC’3’ . EGR1 promotes commitment to the macrophage lineage at the expense of granulocytic lineage [301, 302] and has been shown to be essential for myeloblast differentiation into monocytes/macrophages [303, 304]. Treatment of mouse bone marrow cells with CSF-1 has been shown to induce
5.3.2. Growth factor independence 1 (Gfi1)
Growth factor independence 1 (GFI1) is a zinc finger transcription factor comprised of an N-terminal Snail/Gfi1 (SNAG) domain that is involved in recruiting proteins to modify histones, and a C-terminal domain containing six zinc fingers involved in DNA recognition .
In zebrafish, two
5.3.3. Interferon response factor-8 (IRF-8)
Interferon response factor-8 (IRF-8, also known as ICSBP) is one out of nine members of the IRF transcription factor family and is characterized by an N-terminal DNA binding domain and a C-terminus IRF association domain that can associate with other IRF or Ets transcription family members [316, 317]. IRF8-/- mice and BXH-2 mice with a mutation in their IRF association domain show a drastic expansion of granulocytes at the expense of macrophages [318, 319]. Enforced expression of
The homologue of
In addition to the previously described role of MAFB in HSCs and CMPs (see section 4.3.1),
Studies examining the role of MAFB in teleost myelopoiesis are limited. In the goldfish PKM system, a
Myelopoiesis is an orchestration of a multitude of growth factors and transcription factors that control cell fate decisions and differentiation along a chosen cell lineage. It is evident that there exists some functional redundancy in the action of myelopoietic growth factors, most likely put in place to ensure the production of these critical innate immune cells. Studies have focused on examining the regulation of myelopoiesis in the mouse model system, and have only just begun in the teleost model system.
The divergence of teleosts and mammals occurred approximately 400-450 Mya, thus teleosts represent one of the most basal groups of vertebrates . Comparison of soluble factors and their receptors in teleosts and mammals show retention of many of important hematopoietic growth factors and receptors, including PDGFR [250, 251, 327], c-KIT [128-130], FLT3 (accession number DQ317446), CSF-1R [20, 250-254], GCSFR [292, 293], and their ligands PDGF , KIT ligand [128, 130, 131], CSF-1 [246, 249], and GCSF [290-292], although FLK2, the ligand to FLT3, has not yet been reported. However, it appears that teleosts do not possess the key myeloid growth factors IL-3 and GM-CSF, and their cognate receptors. In addition, teleosts possess all of the TF families required for hematopoiesis in higher vertebrates, reviewed in [28, 30]. Based on studies performed to date, the regulation of hematopoiesis is largely similar between mammals and teleosts. However, teleosts often possess a number of gene duplications for many of the soluble factors, receptors, and to some extent, transcription factors as a result of a teleost-specific whole genome duplication predicted to have occurred approximately 350 Mya, and is believed to be responsible for the radiation of teleosts [329, 330]. Many of these teleost genes are rapidly evolving, often undergoing sub-functionalization or neo-functionalization making the identification of teleost orthologues difficult. By developing an understanding of the soluble mediators, receptors, and the intracellular machinery that govern teleost myelopoiesis, we may be better equipped to develop strategies to promote host defense against pathogens, particularly in aquaculture in which fish are predisposed to infection.
This work was supported by a grant from Natural Sciences and Engineering Research Council of Canada (NSERC) to MB. BAK was supported by NSERC and Alberta Ingenuity Fund (AIF) doctoral scholarships, FK was supported by a Japan Society for the Promotion of Science (JSPS) research fellowship.
List of abbreviations:
Martinez-Agosto JA, Mikkola HK, Hartenstein V, Banerjee U. The hematopoietic stem cell and its niche: a comparative view. Genes and Development 2007;21(23) 3044-60.
Yoder JA, Nielsen ME, Amemiya CT, Litman GW. Zebrafish as an immunological model system. Microbes and Infection 2002;4(14) 1469-78.
Zhang C, Patient R, Liu F. Hematopoietic stem cell development and regulatory signaling in zebrafish. Biochimica et Biophysica Acta 2012.
Renshaw SA, Trede NS. A model 450 million years in the making: zebrafish and vertebrate immunity. Disease Models and Mechanisms 2012;5(1) 38-47.
Jing L, Zon LI. Zebrafish as a model for normal and malignant hematopoiesis. Disease Models and Mechanisms 2011;4(4) 433-8.
Zhong R, Astle CM, Harrison DE. Distinct developmental patterns of short-term and long-term functioning lymphoid and myeloid precursors defined by competitive limiting dilution analysis in vivo. Journal of Immunology 1996;157(1) 138-45.
Osawa M, Hanada K, Hamada H, Nakauchi H. Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell. Science 1996;273(5272) 242-5.
Wognum AW, Eaves AC, Thomas TE. Identification and isolation of hematopoietic stem cells. Archives of medical research 2003;34(6) 461-75.
Szilvassy SJ. The biology of hematopoietic stem cells. Archives of medical research 2003;34(6) 446-60.
Nakanishi T. Histocompatibility analyses in tetraploids induced from clonal triploid crucian carp and in gynogenetic diploid goldfish. Journal of Fish Biology 1987;3135-40.
Nakanishi T, Ototake M. The graft-versus-host reaction (GVHR) in the ginbuna crucian carp, Carassius auratus langsdorfii. Developmental and Comparative Immunology 1999;23(1) 15-26.
Nakanishi T, Okamoto N. Cell-mediated cytotoxicity in isogeneic ginbuna crucian carp. Fish and Shellfish Immunology 1999;9(4) 259-67.
Fischer U, Ototake M, Nakanishi T. Life span of circulating blood cells in ginbuna crucian carp ( Carassius auratus langsdorfii). Fish and Shellfish Immunology 1998;8(5) 339-49.
Neumann NF, Barreda D, Belosevic M. Production of a macrophage growth factor(s) by a goldfish macrophage cell line and macrophages derived from goldfish kidney leukocytes. Developmental and Comparative Immunology 1998;22(4) 417-32.
Neumann NF, Barreda DR, Belosevic M. Generation and functional analysis of distinct macrophage sub-populations from goldfish ( Carassius auratus L.) kidney leukocyte cultures. Fish and Shellfish Immunology 2000;10(1) 1-20.
Katzenback BA, Belosevic M. Isolation and functional characterization of neutrophil-like cells, from goldfish ( Carassius auratusL.) kidney. Developmental and Comparative Immunology 2009;33(4) 601-11.
Barreda DR, Neumann NF, Belosevic M. Flow cytometric analysis of PKH26-labeled goldfish kidney-derived macrophages. Developmental and Comparative Immunology 2000;24(4) 395-406.
Barreda DR, Belosevic M. Characterisation of growth enhancing factor production in different phases of in vitrofish macrophage development. Fish and Shellfish Immunology 2001;11(2) 169-85.
Barreda DR, Hanington PC, Walsh CK, Wong P, Belosevic M. Differentially expressed genes that encode potential markers of goldfish macrophage development in vitro. Developmental and Comparative Immunology 2004;28(7-8) 727-46.
Barreda DR, Hanington PC, Stafford JL, Belosevic M. A novel soluble form of the CSF-1 receptor inhibits proliferation of self-renewing macrophages of goldfish ( Carassius auratusL.). Developmental and Comparative Immunology 2005;29(10) 879-94.
Walsh JG, Barreda DR, Belosevic M. Cloning and expression analysis of goldfish ( Carassius auratusL.) prominin. Fish and Shellfish Immunology 2007;22(4) 308-17.
Hanington PC, Barreda DR, Belosevic M. A novel hematopoietic granulin induces proliferation of goldfish ( Carassius auratusL.) macrophages. Journal of Biological Chemistry 2006;281(15) 9963-70.
Herbomel P, Thisse B, Thisse C. Ontogeny and behaviour of early macrophages in the zebrafish embryo. Development 1999;126(17) 3735-45.
Le Guyader D, Redd MJ, Colucci-Guyon E, Murayama E, Kissa K, Briolat V, et al. Origins and unconventional behavior of neutrophils in developing zebrafish. Blood 2008;111(1) 132-41.
Detrich HW, 3rd, Kieran MW, Chan FY, Barone LM, Yee K, Rundstadler JA, et al. Intraembryonic hematopoietic cell migration during vertebrate development. Proceedings of the National Academy of Science U S A 1995;92(23) 10713-7.
Jin H, Xu J, Wen Z. Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development. Blood 2007;109(12) 5208-14.
Bertrand JY, Kim AD, Violette EP, Stachura DL, Cisson JL, Traver D. Definitive hematopoiesis initiates through a committed erythromyeloid progenitor in the zebrafish embryo. Development 2007;134(23) 4147-56.
Davidson AJ, Zon LI. The 'definitive' (and 'primitive') guide to zebrafish hematopoiesis. Oncogene 2004;23(43) 7233-46.
Chen AT, Zon LI. Zebrafish blood stem cells. J Cell Biochem 2009;108(1) 35-42.
de Jong JL, Zon LI. Use of the zebrafish system to study primitive and definitive hematopoiesis. Annual Review of Genetics 2005;39481-501.
Zapata A, Diez B, Cejalvo T, Gutierrez-de Frias C, Cortes A. Ontogeny of the immune system of fish. Fish and Shellfish Immunology 2006;20(2) 126-36.
Rombout JH, Huttenhuis HB, Picchietti S, Scapigliati G. Phylogeny and ontogeny of fish leucocytes. Fish and Shellfish Immunology 2005;19(5) 441-55.
Bertrand JY, Kim AD, Teng S, Traver D. CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis. Development 2008;135(10) 1853-62.
Murayama E, Kissa K, Zapata A, Mordelet E, Briolat V, Lin HF, et al. Tracing hematopoietic precursor migration to successive hematopoietic organs during zebrafish development. Immunity 2006;25(6) 963-75.
Willett CE, Cortes A, Zuasti A, Zapata AG. Early hematopoiesis and developing lymphoid organs in the zebrafish. Dev Dyn 1999;214(4) 323-36.
Zapata A. Ultrastructural study of the teleost fish kidney. Developmental and Comparative Immunology 1979;3(1) 55-65.
Traver D, Paw BH, Poss KD, Penberthy WT, Lin S, Zon LI. Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants. Nature Immunology 2003;4(12) 1238-46.
Traver D, Winzeler A, Stern HM, Mayhall EA, Langenau DM, Kutok JL, et al. Effects of lethal irradiation in zebrafish and rescue by hematopoietic cell transplantation. Blood 2004;104(5) 1298-305.
Kobayashi I, Saito K, Moritomo T, Araki K, Takizawa F, Nakanishi T. Characterization and localization of side population (SP) cells in zebrafish kidney hematopoietic tissue. Blood 2008;111(3) 1131-7.
Kobayashi I, Sekiya M, Moritomo T, Ototake M, Nakanishi T. Demonstration of hematopoietic stem cells in ginbuna carp ( Carassius auratus langsdorfii) kidney. Developmental and Comparative Immunology 2006;30(11) 1034-46.
Kobayashi I, Moritomo T, Ototake M, Nakanishi T. Isolation of side population cells from ginbuna carp ( Carassius auratus langsdorfii) kidney hematopoietic tissues. Developmental and Comparative Immunology 2007;31(7) 696-707.
Kobayashi I, Kusakabe H, Toda H, Moritomo T, Takahashi T, Nakanishi T. In vivocharacterization of primitive hematopoietic cells in clonal ginbuna crucian carp ( Carassius auratus langsdorfii). Veterinary Immunology and Immunopathology 2008;126(1-2) 74-82.
Kobayashi I, Kuniyoshi S, Saito K, Moritomo T, Takahashi T, Nakanishi T. Long-term hematopoietic reconstitution by transplantation of kidney hematopoietic stem cells in lethally irradiated clonal ginbuna crucian carp ( Carassius auratus langsdorfii). Developmental and Comparative Immunology 2008;32(8) 957-65.
Moritomo T, Asakura N, Sekiya M, Ototake M, Inoue Y, Nakanishi T. Cell culture of clonal ginbuna crucian carp hematopoietic cells: differentiation of cultured cells into erythrocytes in vivo. Developmental and Comparative Immunology 2004;28(9) 863-9.
Lieschke GJ, Oates AC, Crowhurst MO, Ward AC, Layton JE. Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish. Blood 2001;98(10) 3087-96.
Rieger MA, Hoppe PS, Smejkal BM, Eitelhuber AC, Schroeder T. Hematopoietic cytokines can instruct lineage choice. Science 2009;325(5937) 217-8.
Metcalf D. Lineage commitment and maturation in hematopoietic cells: the case for extrinsic regulation. Blood 1998;92(2) 345-7; discussion 52.
Metcalf D. Hematopoietic cytokines. Blood 2008;111(2) 485-91.
Zhu J, Emerson SG. Hematopoietic cytokines, transcription factors and lineage commitment. Oncogene 2002;21(21) 3295-313.
Cantor AB, Orkin SH. Transcriptional regulation of erythropoiesis: an affair involving multiple partners. Oncogene 2002;21(21) 3368-76.
Friedman AD. Transcriptional regulation of granulocyte and monocyte development. Oncogene 2002;21(21) 3377-90.
Ye M, Graf T. Early decisions in lymphoid development. Current Opinion in Immunology 2007;19(2) 123-8.
Wang LD, Wagers AJ. Dynamic niches in the origination and differentiation of haematopoietic stem cells. Nature Reviews Molecular Cell Biology 2011;12(10) 643-55.
Lai AY, Kondo M. Asymmetrical lymphoid and myeloid lineage commitment in multipotent hematopoietic progenitors. Journal of Experimental Medicine 2006;203(8) 1867-73.
Adolfsson J, Mansson R, Buza-Vidas N, Hultquist A, Liuba K, Jensen CT, et al. Identification of Flt3+ lympho-myeloid stem cells lacking erythro-megakaryocytic potential a revised road map for adult blood lineage commitment. Cell 2005;121(2) 295-306.
Chi AW, Chavez A, Xu L, Weber BN, Shestova O, Schaffer A, et al. Identification of Flt3CD150 myeloid progenitors in adult mouse bone marrow that harbor T lymphoid developmental potential. Blood 2011;118(10) 2723-32.
Ng SY, Yoshida T, Zhang J, Georgopoulos K. Genome-wide lineage-specific transcriptional networks underscore Ikaros-dependent lymphoid priming in hematopoietic stem cells. Immunity 2009;30(4) 493-507.
Pronk CJ, Rossi DJ, Mansson R, Attema JL, Norddahl GL, Chan CK, et al. Elucidation of the phenotypic, functional, and molecular topography of a myeloerythroid progenitor cell hierarchy. Cell Stem Cell 2007;1(4) 428-42.
Montecino-Rodriguez E, Leathers H, Dorshkind K. Bipotential B-macrophage progenitors are present in adult bone marrow. Nature Immunology 2001;2(1) 83-8.
Kondo M, Weissman IL, Akashi K. Identification of clonogenic common lymphoid progenitors in mouse bone marrow. Cell 1997;91(5) 661-72.
Luc S, Buza-Vidas N, Jacobsen SE. Biological and molecular evidence for existence of lymphoid-primed multipotent progenitors. Annals of the New York Academy of Sciences 2007;110689-94.
Katsura Y, Kawamoto H. Stepwise lineage restriction of progenitors in lympho-myelopoiesis. International Reviews of Immunology 2001;20(1) 1-20.
Kawamoto H, Ohmura K, Katsura Y. Direct evidence for the commitment of hematopoietic stem cells to T, B and myeloid lineages in murine fetal liver. International Immunology 1997;9(7) 1011-9.
Lu M, Kawamoto H, Katsube Y, Ikawa T, Katsura Y. The common myelolymphoid progenitor: a key intermediate stage in hemopoiesis generating T and B cells. Journal of Immunology 2002;169(7) 3519-25.
Wada H, Masuda K, Satoh R, Kakugawa K, Ikawa T, Katsura Y, et al. Adult T-cell progenitors retain myeloid potential. Nature 2008;452(7188) 768-72.
Kawamoto H, Katsura Y. A new paradigm for hematopoietic cell lineages: revision of the classical concept of the myeloid-lymphoid dichotomy. Trends in Immunology 2009;30(5) 193-200.
Kawamoto H, Ikawa T, Masuda K, Wada H, Katsura Y. A map for lineage restriction of progenitors during hematopoiesis: the essence of the myeloid-based model. Immunological reviews 2010;238(1) 23-36.
Li J, Barreda DR, Zhang YA, Boshra H, Gelman AE, LaPatra S, et al. B lymphocytes from early vertebrates have potent phagocytic and microbicidal abilities. Nature Immunology 2006;7(10) 1116-24.
Katakura F, Yamaguchi T, Yoshida M, Moritomo T, Nakanishi T. Demonstration of T cell and macrophage progenitors in carp ( Cyprinus carpio) kidney hematopoietic tissues. Development of clonal assay system for carp hematopoietic cells. Developmental and Comparative Immunology 2010;34(6) 685-9.
Huang E, Nocka K, Beier DR, Chu TY, Buck J, Lahm HW, et al. The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus. Cell 1990;63(1) 225-33.
Copeland NG, Gilbert DJ, Cho BC, Donovan PJ, Jenkins NA, Cosman D, et al. Mast cell growth factor maps near the steel locus on mouse chromosome 10 and is deleted in a number of steel alleles. Cell 1990;63(1) 175-83.
Witte ON. Steel locus defines new multipotent growth factor. Cell 1990;63(1) 5-6.
Zhang Z, Zhang R, Joachimiak A, Schlessinger J, Kong XP. Crystal structure of human stem cell factor: implication for stem cell factor receptor dimerization and activation. Proceedings of the National Academy of Science U S A 2000;97(14) 7732-7.
Russell ES. Hereditary anemias of the mouse: a review for geneticists. Advances in Genetics 1979;20357-459.
Dexter TM, Moore MA. In vitroduplication and "cure" of haemopoietic defects in genetically anaemic mice. Nature 1977;269(5627) 412-4.
Anderson DM, Lyman SD, Baird A, Wignall JM, Eisenman J, Rauch C, et al. Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms. Cell 1990;63(1) 235-43.
Langley KE, Bennett LG, Wypych J, Yancik SA, Liu XD, Westcott KR, et al. Soluble stem cell factor in human serum. Blood 1993;81(3) 656-60.
Arakawa T, Yphantis DA, Lary JW, Narhi LO, Lu HS, Prestrelski SJ, et al. Glycosylated and unglycosylated recombinant-derived human stem cell factors are dimeric and have extensive regular secondary structure. Journal of Biological Chemistry 1991;266(28) 18942-8.
Pandiella A, Bosenberg MW, Huang EJ, Besmer P, Massague J. Cleavage of membrane-anchored growth factors involves distinct protease activities regulated through common mechanisms. Journal of Biological Chemistry 1992;267(33) 24028-33.
Tajima Y, Moore MA, Soares V, Ono M, Kissel H, Besmer P. Consequences of exclusive expression in vivo of Kit-ligand lacking the major proteolytic cleavage site. Proceedings of the National Academy of Science U S A 1998;95(20) 11903-8.
Ashman LK. The biology of stem cell factor and its receptor C-kit. International Journal of Biochemistry and Cell Biology 1999;31(10) 1037-51.
Tajima Y, Huang EJ, Vosseller K, Ono M, Moore MA, Besmer P. Role of dimerization of the membrane-associated growth factor kit ligand in juxtacrine signaling: the Sl17H mutation affects dimerization and stability-phenotypes in hematopoiesis. Journal of Experimental Medicine 1998;187(9) 1451-61.
Besmer P, Murphy JE, George PC, Qiu FH, Bergold PJ, Lederman L, et al. A new acute transforming feline retrovirus and relationship of its oncogene v-kit with the protein kinase gene family. Nature 1986;320(6061) 415-21.
Yarden Y, Kuang WJ, Yang-Feng T, Coussens L, Munemitsu S, Dull TJ, et al. Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO Journal 1987;6(11) 3341-51.
Qiu FH, Ray P, Brown K, Barker PE, Jhanwar S, Ruddle FH, et al. Primary structure of c-kit: relationship with the CSF-1/PDGF receptor kinase family--oncogenic activation of v-kit involves deletion of extracellular domain and C terminus. EMBO Journal 1988;7(4) 1003-11.
Ullrich A, Schlessinger J. Signal transduction by receptors with tyrosine kinase activity. Cell 1990;61(2) 203-12.
Rosnet O, Schiff C, Pebusque MJ, Marchetto S, Tonnelle C, Toiron Y, et al. Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells. Blood 1993;82(4) 1110-9.
Galli SJ, Zsebo KM, Geissler EN. The kit ligand, stem cell factor. Advances in Immunology 1994;551-96.
Ratajczak J, Machalinski B, Majka M, Kijowski J, Marlicz W, Rozmyslowicz T, et al. Evidence that human haematopoietic stem cells (HSC) do not reside within the CD34+KIT- cell population. Annnals of Transplantation 1999;4(1) 22-30.
Cairns LA, Moroni E, Levantini E, Giorgetti A, Klinger FG, Ronzoni S, et al. Kit regulatory elements required for expression in developing hematopoietic and germ cell lineages. Blood 2003;102(12) 3954-62.
Ikuta K, Weissman IL. Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. Proceedings of the National Academy of Science U S A 1992;89(4) 1502-6.
Cerisoli F, Cassinelli L, Lamorte G, Citterio S, Bertolotti F, Magli MC, et al. Green fluorescent protein transgene driven by Kit regulatory sequences is expressed in hematopoietic stem cells. Haematologica 2009;94(3) 318-25.
Okayama Y, Kawakami T. Development, migration, and survival of mast cells. Immunologic Research 2006;34(2) 97-115.
Cole SR, Aylett GW, Harvey NL, Cambareri AC, Ashman LK. Increased expression of c-Kit or its ligand Steel Factor is not a common feature of adult acute myeloid leukaemia. Leukemia 1996;10(2) 288-96.
Turner AM, Bennett LG, Lin NL, Wypych J, Bartley TD, Hunt RW, et al. Identification and characterization of a soluble c-kit receptor produced by human hematopoietic cell lines. Blood 1995;85(8) 2052-8.
Yee NS, Langen H, Besmer P. Mechanism of kit ligand, phorbol ester, and calcium-induced down-regulation of c-kit receptors in mast cells. Journal of Biological Chemistry 1993;268(19) 14189-201.
Broudy VC, Kovach NL, Bennett LG, Lin N, Jacobsen FW, Kidd PG. Human umbilical vein endothelial cells display high-affinity c-kit receptors and produce a soluble form of the c-kit receptor. Blood 1994;83(8) 2145-52.
Dahlen DD, Lin NL, Liu YC, Broudy VC. Soluble Kit receptor blocks stem cell factor bioactivity in vitro. Leukemia Research 2001;25(5) 413-21.
Jiang X, Gurel O, Mendiaz EA, Stearns GW, Clogston CL, Lu HS, et al. Structure of the active core of human stem cell factor and analysis of binding to its receptor kit. EMBO Journal 2000;19(13) 3192-203.
Philo JS, Wen J, Wypych J, Schwartz MG, Mendiaz EA, Langley KE. Human stem cell factor dimer forms a complex with two molecules of the extracellular domain of its receptor, Kit. Journal of Biological Chemistry 1996;271(12) 6895-902.
Broudy VC. Stem cell factor and hematopoiesis. Blood 1997;90(4) 1345-64.
Blechman JM, Lev S, Brizzi MF, Leitner O, Pegoraro L, Givol D, et al. Soluble c-kit proteins and antireceptor monoclonal antibodies confine the binding site of the stem cell factor. Journal of Biological Chemistry 1993;268(6) 4399-406.
Lev S, Blechman J, Nishikawa S, Givol D, Yarden Y. Interspecies molecular chimeras of kit help define the binding site of the stem cell factor. Molecular and Cellular Biology 1993;13(4) 2224-34.
Yuzawa S, Opatowsky Y, Zhang Z, Mandiyan V, Lax I, Schlessinger J. Structural basis for activation of the receptor tyrosine kinase KIT by stem cell factor. Cell 2007;130(2) 323-34.
Narhi LO, Wypych J, Li T, Langley KE, Arakawa T. Changes in conformation and stability upon SCF/sKit complex formation. Journal of Protein Chemistry 1998;17(5) 387-96.
Linnekin D. Early signaling pathways activated by c-Kit in hematopoietic cells. International Journal of Biochemistry and Cell Biology 1999;31(10) 1053-74.
Roskoski R, Jr. Signaling by Kit protein-tyrosine kinase--the stem cell factor receptor. Biochemical and Biophysical Research Communications 2005;337(1) 1-13.
Kent D, Copley M, Benz C, Dykstra B, Bowie M, Eaves C. Regulation of hematopoietic stem cells by the steel factor/KIT signaling pathway. Clinical Cancer Research 2008;14(7) 1926-30.
Mauduit C, Hamamah S, Benahmed M. Stem cell factor/c-kit system in spermatogenesis. Humum Reproduction Update 1999;5(5) 535-45.
Besmer P, Manova K, Duttlinger R, Huang EJ, Packer A, Gyssler C, et al. The kit-ligand (steel factor) and its receptor c-kit/W: pleiotropic roles in gametogenesis and melanogenesis. Journal of Reproduction and Development Supplement 1993125-37.
Bedell MA, Mahakali Zama A. Genetic analysis of Kit ligand functions during mouse spermatogenesis. Journal of Adrology 2004;25(2) 188-99.
Reid K, Nishikawa S, Bartlett PF, Murphy M. Steel factor directs melanocyte development in vitro through selective regulation of the number of c-kit+ progenitors. Developmental Biology 1995;169(2) 568-79.
Wehrle-Haller B, Weston JA. Soluble and cell-bound forms of steel factor activity play distinct roles in melanocyte precursor dispersal and survival on the lateral neural crest migration pathway. Development 1995;121(3) 731-42.
Wehrle-Haller B. The role of Kit-ligand in melanocyte development and epidermal homeostasis. Pigment Cell Research 2003;16(3) 287-96.
Yee NS, Paek I, Besmer P. Role of kit-ligand in proliferation and suppression of apoptosis in mast cells: basis for radiosensitivity of white spotting and steel mutant mice. Journal of Experimental Medicine 1994;179(6) 1777-87.
Serve H, Yee NS, Stella G, Sepp-Lorenzino L, Tan JC, Besmer P. Differential roles of PI3-kinase and Kit tyrosine 821 in Kit receptor-mediated proliferation, survival and cell adhesion in mast cells. EMBO Journal 1995;14(3) 473-83.
Wershil BK, Tsai M, Geissler EN, Zsebo KM, Galli SJ. The rat c-kit ligand, stem cell factor, induces c-kit receptor-dependent mouse mast cell activation in vivo. Evidence that signaling through the c-kit receptor can induce expression of cellular function. Journal of Experimental Medicine 1992;175(1) 245-55.
Tanaka A, Arai K, Kitamura Y, Matsuda H. Matrix metalloproteinase-9 production, a newly identified function of mast cell progenitors, is downregulated by c-kit receptor activation. Blood 1999;94(7) 2390-5.
Lukacs NW, Kunkel SL, Strieter RM, Evanoff HL, Kunkel RG, Key ML, et al. The role of stem cell factor (c-kit ligand) and inflammatory cytokines in pulmonary mast cell activation. Blood 1996;87(6) 2262-8.
Reber L, Da Silva CA, Frossard N. Stem cell factor and its receptor c-Kit as targets for inflammatory diseases. European Journal of Pharmacology 2006;533(1-3) 327-40.
Ogawa M, Matsuzaki Y, Nishikawa S, Hayashi S, Kunisada T, Sudo T, et al. Expression and function of c-kit in hemopoietic progenitor cells. Journal of Experimental Medicine 1991;174(1) 63-71.
Keller JR, Ortiz M, Ruscetti FW. Steel factor (c-kit ligand) promotes the survival of hematopoietic stem/progenitor cells in the absence of cell division. Blood 1995;86(5) 1757-64.
Thoren LA, Liuba K, Bryder D, Nygren JM, Jensen CT, Qian H, et al. Kit regulates maintenance of quiescent hematopoietic stem cells. J Immunol 2008;180(4) 2045-53.
Mantel C, Hendrie P, Broxmeyer HE. Steel factor regulates cell cycle asymmetry. Stem Cells 2001;19(6) 483-91.
Zsebo KM, Wypych J, McNiece IK, Lu HS, Smith KA, Karkare SB, et al. Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver--conditioned medium. Cell 1990;63(1) 195-201.
Bernstein ID, Andrews RG, Zsebo KM. Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin- cells, and the generation of colony-forming cell progeny from CD34+lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor. Blood 1991;77(11) 2316-21.
Okumura N, Tsuji K, Ebihara Y, Tanaka I, Sawai N, Koike K, et al. Chemotactic and chemokinetic activities of stem cell factor on murine hematopoietic progenitor cells. Blood 1996;87(10) 4100-8.
Parichy DM, Rawls JF, Pratt SJ, Whitfield TT, Johnson SL. Zebrafish sparse corresponds to an orthologue of c-kit and is required for the morphogenesis of a subpopulation of melanocytes, but is not essential for hematopoiesis or primordial germ cell development. Development 1999;126(15) 3425-36.
Mellgren EM, Johnson SL. kitb, a second zebrafish ortholog of mouse Kit. Developmental Genes and Evolution 2005;215(9) 470-77.
Katzenback BA, Belosevic M. Molecular and functional characterization of kita and kitla of the goldfish ( Carassius auratusL.). Developmental and Comparative Immunology 2009;33(11) 1165-75.
Hultman KA, Bahary N, Zon LI, Johnson SL. Gene Duplication of the zebrafish kit ligand and partitioning of melanocyte development functions to kit ligand a. PLoS Genetics 2007;3(1) e17.
Yao K, Ge W. Kit system in the zebrafish ovary: evidence for functional divergence of two isoforms of kit (kita and kitb) and kit ligand (kitlga and kitlgb) during folliculogenesis. Biology of Reproduction 2010;82(6) 1216-26.
Cooper CD, Linbo TH, Raible DW. Kit and foxd3 genetically interact to regulate melanophore survival in zebrafish. Developmental Dynamics 2009;238(4) 875-86.
Mellgren EM, Johnson SL. A requirement for kit in embryonic zebrafish melanocyte differentiation is revealed by melanoblast delay. Developmental Genes and Evolution 2004;214(10) 493-502.
Rawls JF, Johnson SL. Requirements for the kit receptor tyrosine kinase during regeneration of zebrafish fin melanocytes. Development 2001;128(11) 1943-9.
Yamada T, Okauchi M, Araki K. Origin of adult-type pigment cells forming the asymmetric pigment pattern, in Japanese flounder ( Paralichthys olivaceus). Developmental Dynamics 2010;239(12) 3147-62.
Mills MG, Nuckels RJ, Parichy DM. Deconstructing evolution of adult phenotypes: genetic analyses of kit reveal homology and evolutionary novelty during adult pigment pattern development of Danio fishes. Development 2007;134(6) 1081-90.
Ihle JN, Keller J, Oroszlan S, Henderson LE, Copeland TD, Fitch F, et al. Biologic properties of homogeneous interleukin 3. I. Demonstration of WEHI-3 growth factor activity, mast cell growth factor activity, p cell-stimulating factor activity, colony-stimulating factor activity, and histamine-producing cell-stimulating factor activity. Journal of Immunology 1983;131(1) 282-7.
Yang YC, Ciarletta AB, Temple PA, Chung MP, Kovacic S, Witek-Giannotti JS, et al. Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3. Cell 1986;47(1) 3-10.
Rennick DM, Lee FD, Yokota T, Arai KI, Cantor H, Nabel GJ. A cloned MCGF cDNA encodes a multilineage hematopoietic growth factor: multiple activities of interleukin 3. Journal of Immunology 1985;134(2) 910-4.
Spivak JL, Smith RR, Ihle JN. Interleukin 3 promotes the in vitroproliferation of murine pluripotent hematopoietic stem cells. Journal of Clinical Investigation 1985;76(4) 1613-21.
Quesenberry PJ, Ihle JN, McGrath E. The effect of interleukin 3 and GM-CSA-2 on megakaryocyte and myeloid clonal colony formation. Blood 1985;65(1) 214-7.
van Leeuwen BH, Martinson ME, Webb GC, Young IG. Molecular organization of the cytokine gene cluster, involving the human IL-3, IL-4, IL-5, and GM-CSF genes, on human chromosome 5. Blood 1989;73(5) 1142-8.
Barreda DR, Hanington PC, Belosevic M. Regulation of myeloid development and function by colony stimulating factors. Developmental and Comparative Immunology 2004;28(5) 509-54.
Martinez-Moczygemba M, Huston DP. Biology of common beta receptor-signaling cytokines: IL-3, IL-5, and GM-CSF. Journal of Allergy and Clinical Immunology 2003;112(4) 653-65; quiz 66.
Scott CL, Begley CG. The beta common chain (beta c) of the granulocyte macrophage-colony stimulating factor, interleukin-3 and interleukin-5 receptors. International Journal of Biochemistry and Cell Biology 1999;31(10) 1011-5.
Gasson JC, Weisbart RH, Kaufman SE, Clark SC, Hewick RM, Wong GG, et al. Purified human granulocyte-macrophage colony-stimulating factor: direct action on neutrophils. Science 1984;226(4680) 1339-42.
Cantrell MA, Anderson D, Cerretti DP, Price V, McKereghan K, Tushinski RJ, et al. Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor. Proceedings of the National Academy of Science U S A 1985;82(18) 6250-4.
Otsuka T, Miyajima A, Brown N, Otsu K, Abrams J, Saeland S, et al. Isolation and characterization of an expressible cDNA encoding human IL-3. Induction of IL-3 mRNA in human T cell clones. Journal of Immunology 1988;140(7) 2288-95.
Broudy VC, Kaushansky K, Segal GM, Harlan JM, Adamson JW. Tumor necrosis factor type alpha stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor. Proceedings of the National Academy of Science U S A 1986;83(19) 7467-71.
Munker R, Gasson J, Ogawa M, Koeffler HP. Recombinant human TNF induces production of granulocyte-monocyte colony-stimulating factor. Nature 1986;323(6083) 79-82.
Kimura A, Rieger MA, Simone JM, Chen W, Wickre MC, Zhu BM, et al. The transcription factors STAT5A/B regulate GM-CSF-mediated granulopoiesis. Blood 2009;114(21) 4721-8.
Gomez-Cambronero J, Horn J, Paul CC, Baumann MA. Granulocyte-macrophage colony-stimulating factor is a chemoattractant cytokine for human neutrophils: involvement of the ribosomal p70 S6 kinase signaling pathway. Journal of Immunology 2003;171(12) 6846-55.
Khajah M, Millen B, Cara DC, Waterhouse C, McCafferty DM. Granulocyte-macrophage colony-stimulating factor (GM-CSF): a chemoattractive agent for murine leukocytes in vivo. Journal of Leukocyte Biology 2011;89(6) 945-53.
Fossati G, Mazzucchelli I, Gritti D, Ricevuti G, Edwards SW, Moulding DA, et al. In vitro effects of GM-CSF on mature peripheral blood neutrophils. International Journal of Molecular Medicine 1998;1(6) 943-51.
Watowich SS, Liu YJ. Mechanisms regulating dendritic cell specification and development. Immunological Reviews 2010;238(1) 76-92.
Schmid MA, Kingston D, Boddupalli S, Manz MG. Instructive cytokine signals in dendritic cell lineage commitment. Immunological Reviews 2010;234(1) 32-44.
Hercus TR, Thomas D, Guthridge MA, Ekert PG, King-Scott J, Parker MW, et al. The granulocyte-macrophage colony-stimulating factor receptor: linking its structure to cell signaling and its role in disease. Blood 2009;114(7) 1289-98.
Laiosa CV, Stadtfeld M, Graf T. Determinants of lymphoid-myeloid lineage diversification. Annual Review of Immunology 2006;24705-38.
Iwasaki H, Akashi K. Myeloid lineage commitment from the hematopoietic stem cell. Immunity 2007;26(6) 726-40.
Rosenbauer F, Tenen DG. Transcription factors in myeloid development: balancing differentiation with transformation. Nature Reviews Immunology 2007;7(2) 105-17.
Hanington PC, Tam J, Katzenback BA, Hitchen SJ, Barreda DR, Belosevic M. Development of macrophages of cyprinid fish. Developmental and Comparative Immunology 2009;33(4) 411-29.
Sarrazin S, Mossadegh-Keller N, Fukao T, Aziz A, Mourcin F, Vanhille L, et al. MafB restricts M-CSF-dependent myeloid commitment divisions of hematopoietic stem cells. Cell 2009;138(2) 300-13.
Sieweke MH, Tekotte H, Frampton J, Graf T. MafB is an interaction partner and repressor of Ets-1 that inhibits erythroid differentiation. Cell 1996;85(1) 49-60.
Kajihara M, Kawauchi S, Kobayashi M, Ogino H, Takahashi S, Yasuda K. Isolation, characterization, and expression analysis of zebrafish large Mafs. Journal of Biochemistry 2001;129(1) 139-46.
Landschulz WH, Johnson PF, McKnight SL. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science 1988;240(4860) 1759-64.
Lekstrom-Himes J, Xanthopoulos KG. Biological role of the CCAAT/enhancer-binding protein family of transcription factors. Journal of Biological Chemistry 1998;273(44) 28545-8.
Lyons SE, Shue BC, Lei L, Oates AC, Zon LI, Liu PP. Molecular cloning, genetic mapping, and expression analysis of four zebrafish c/ebp genes. Gene 2001;281(1-2) 43-51.
Fujiki K, Gerwick L, Bayne CJ, Mitchell L, Gauley J, Bols N, et al. Molecular cloning and characterization of rainbow trout ( Oncorhynchus mykiss) CCAAT/enhancer binding protein beta. Immunogenetics 2003;55(4) 253-61.
Tucker CS, Hirono I, Aoki T. Molecular cloning and expression of CCAAT/enhancer binding proteins in Japanese flounder Paralichthys olivaceus. Developmental and Comparative Immunology 2002;26(3) 271-82.
Hikima J, Ohtani M, Kondo H, Hirono I, Jung TS, Aoki T. Characterization and gene expression of transcription factors, PU.1 and C/EBPalpha driving transcription from the tumor necrosis factor alpha promoter in Japanese flounder, Paralichthys olivaceus. Developmental and Comparative Immunology 2011;35(3) 304-13.
Traver D, Miyamoto T, Christensen J, Iwasaki-Arai J, Akashi K, Weissman IL. Fetal liver myelopoiesis occurs through distinct, prospectively isolatable progenitor subsets. Blood 2001;98(3) 627-35.
Hohaus S, Petrovick MS, Voso MT, Sun Z, Zhang DE, Tenen DG. PU.1 (Spi-1) and C/EBP alpha regulate expression of the granulocyte-macrophage colony-stimulating factor receptor alpha gene. Molecular and Cellular Biology 1995;15(10) 5830-45.
Heath V, Suh HC, Holman M, Renn K, Gooya JM, Parkin S, et al. C/EBPalpha deficiency results in hyperproliferation of hematopoietic progenitor cells and disrupts macrophage development in vitroand in vivo. Blood 2004;104(6) 1639-47.
Friedman AD. C/EBPalpha induces PU.1 and interacts with AP-1 and NF-kappaB to regulate myeloid development. Blood Cells, Molecules and Diseases 2007;39(3) 340-3.
Zhang P, Iwasaki-Arai J, Iwasaki H, Fenyus ML, Dayaram T, Owens BM, et al. Enhancement of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBP alpha. Immunity 2004;21(6) 853-63.
Laslo P, Spooner CJ, Warmflash A, Lancki DW, Lee HJ, Sciammas R, et al. Multilineage transcriptional priming and determination of alternate hematopoietic cell fates. Cell 2006;126(4) 755-66.
Radomska HS, Huettner CS, Zhang P, Cheng T, Scadden DT, Tenen DG. CCAAT/enhancer binding protein alpha is a regulatory switch sufficient for induction of granulocytic development from bipotential myeloid progenitors. Molecular and Cellular Biology 1998;18(7) 4301-14.
Ford AM, Bennett CA, Healy LE, Towatari M, Greaves MF, Enver T. Regulation of the myeloperoxidase enhancer binding proteins Pu1, C-EBP alpha, -beta, and -delta during granulocyte-lineage specification. Proceedings of the National Academy of Science U S A 1996;93(20) 10838-43.
Oelgeschlager M, Nuchprayoon I, Luscher B, Friedman AD. C/EBP, c-Myb, and PU.1 cooperate to regulate the neutrophil elastase promoter. Molecular and Cellular Biology 1996;16(9) 4717-25.
Smith LT, Hohaus S, Gonzalez DA, Dziennis SE, Tenen DG. PU.1 (Spi-1) and C/EBP alpha regulate the granulocyte colony-stimulating factor receptor promoter in myeloid cells. Blood 1996;88(4) 1234-47.
Liu TX, Rhodes J, Deng M, Hsu K, Radomska HS, Kanki JP, et al. Dominant-interfering C/EBPalpha stimulates primitive erythropoiesis in zebrafish. Experimental Hematology 2007;35(2) 230-9.
Yuan H, Zhou J, Deng M, Zhang Y, Chen Y, Jin Y, et al. Sumoylation of CCAAT/enhancer-binding protein alpha promotes the biased primitive hematopoiesis of zebrafish. Blood 2011;117(26) 7014-20.
Burda P, Laslo P, Stopka T. The role of PU.1 and GATA-1 transcription factors during normal and leukemogenic hematopoiesis. Leukemia 2010;24(7) 1249-57.
Klemsz MJ, McKercher SR, Celada A, Van Beveren C, Maki RA. The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. Cell 1990;61(1) 113-24.
Kim HG, de Guzman CG, Swindle CS, Cotta CV, Gartland L, Scott EW, et al. The ETS family transcription factor PU.1 is necessary for the maintenance of fetal liver hematopoietic stem cells. Blood 2004;104(13) 3894-900.
Iwasaki H, Somoza C, Shigematsu H, Duprez EA, Iwasaki-Arai J, Mizuno S, et al. Distinctive and indispensable roles of PU.1 in maintenance of hematopoietic stem cells and their differentiation. Blood 2005;106(5) 1590-600.
Dakic A, Metcalf D, Di Rago L, Mifsud S, Wu L, Nutt SL. PU.1 regulates the commitment of adult hematopoietic progenitors and restricts granulopoiesis. Journal of Experimental Medicine 2005;201(9) 1487-502.
McKercher SR, Torbett BE, Anderson KL, Henkel GW, Vestal DJ, Baribault H, et al. Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities. EMBO Journal 1996;15(20) 5647-58.
Scott EW, Simon MC, Anastasi J, Singh H. Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages. Science 1994;265(5178) 1573-7.
Nutt SL, Metcalf D, D'Amico A, Polli M, Wu L. Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors. Journal of Experimental Medicine 2005;201(2) 221-31.
Zhang P, Zhang X, Iwama A, Yu C, Smith KA, Mueller BU, et al. PU.1 inhibits GATA-1 function and erythroid differentiation by blocking GATA-1 DNA binding. Blood 2000;96(8) 2641-8.
Rekhtman N, Radparvar F, Evans T, Skoultchi AI. Direct interaction of hematopoietic transcription factors PU.1 and GATA-1: functional antagonism in erythroid cells. Genes and Development 1999;13(11) 1398-411.
Xie Y, Chen C, Hume DA. Transcriptional regulation of c-fms gene expression. Cell Biochemistry and Biophysics 2001;34(1) 1-16.
Tagoh H, Himes R, Clarke D, Leenen PJ, Riggs AD, Hume D, et al. Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells. Genes and Development 2002;16(13) 1721-37.
Gangenahalli GU, Gupta P, Saluja D, Verma YK, Kishore V, Chandra R, et al. Stem cell fate specification: role of master regulatory switch transcription factor PU.1 in differential hematopoiesis. Stem Cells and Devevelopment 2005;14(2) 140-52.
Ito Y, Seto Y, Brannan CI, Copeland NG, Jenkins NA, Fukunaga R, et al. Structural analysis of the functional gene and pseudogene encoding the murine granulocyte colony-stimulating-factor receptor. European Journal of Biochemistry 1994;220(3) 881-91.
Lieschke GJ, Oates AC, Paw BH, Thompson MA, Hall NE, Ward AC, et al. Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: implications for axial patterning. Developmental Bioliology 2002;246(2) 274-95.
Ward AC, McPhee DO, Condron MM, Varma S, Cody SH, Onnebo SM, et al. The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish. Blood 2003;102(9) 3238-40.
Hsu K, Traver D, Kutok JL, Hagen A, Liu TX, Paw BH, et al. The pu.1 promoter drives myeloid gene expression in zebrafish. Blood 2004;104(5) 1291-7.
Rhodes J, Hagen A, Hsu K, Deng M, Liu TX, Look AT, et al. Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish. Developmental Cell 2005;8(1) 97-108.
Galloway JL, Wingert RA, Thisse C, Thisse B, Zon LI. Loss of gata1 but not gata2 converts erythropoiesis to myelopoiesis in zebrafish embryos. Developmental Cell 2005;8(1) 109-16.
Zakrzewska A, Cui C, Stockhammer OW, Benard EL, Spaink HP, Meijer AH. Macrophage-specific gene functions in Spi1-directed innate immunity. Blood 2010;116(3) e1-11.
Bukrinsky A, Griffin KJ, Zhao Y, Lin S, Banerjee U. Essential role of spi-1-like (spi-1l) in zebrafish myeloid cell differentiation. Blood 2009;113(9) 2038-46.
Takahashi K. Development and differentiation of macrophages and related cells: historical review and current concepts. Journal of Clinical and Experimental Hematopathology 2001;411-33.
Auffray C, Sieweke MH, Geissmann F. Blood monocytes: development, heterogeneity, and relationship with dendritic cells. Annual Review of Immunology 2009;27669-92.
Geissmann F, Manz MG, Jung S, Sieweke MH, Merad M, Ley K. Development of monocytes, macrophages, and dendritic cells. Science 2010;327(5966) 656-61.
Ganassin RC, Bols NC. Development of a monocyte/macrophage-like cell line, RTS11, from rainbow trout spleen. Fish and Shellfish Immunology 1998;8457-76.
Stafford JL, McLauchlan PE, Secombes CJ, Ellis AE, Belosevic M. Generation of primary monocyte-like cultures from rainbow trout head kidney leukocytes. Developmental and Comparative Immunology 2001;25(5-6) 447-59.
Stachura DL, Reyes JR, Bartunek P, Paw BH, Zon LI, Traver D. Zebrafish kidney stromal cell lines support multilineage hematopoiesis. Blood 2009;114(2) 279-89.
Stachura DL, Svoboda O, Lau RP, Balla KM, Zon LI, Bartunek P, et al. Clonal analysis of hematopoietic progenitor cells in the zebrafish. Blood 2011;118(5) 1274-82.
Bennett CM, Kanki JP, Rhodes J, Liu TX, Paw BH, Kieran MW, et al. Myelopoiesis in the zebrafish, Danio rerio. Blood 2001;98(3) 643-51.
Crowhurst MO, Layton JE, Lieschke GJ. Developmental biology of zebrafish myeloid cells. International Journal of Developmental Biology 2002;46(4) 483-92.
Zuasti A, Ferrer C. Haemopoiesis in the head kidney of Sparus auratus. Archives of Histology and Cytology 1989;52(3) 249-55.
Abdel-Aziz el SH, Abdu SB, Ali Tel S, Fouad HF. Haemopoiesis in the head kidney of tilapia, Oreochromis niloticus(Teleostei: Cichlidae): a morphological (optical and ultrastructural) study. Fish Physiology and Biochemistry 2010;36(3) 323-36.
Wittamer V, Bertrand JY, Gutschow PW, Traver D. Characterization of the mononuclear phagocyte system in zebrafish. Blood 2011;117(26) 7126-35.
Feng Y, Santoriello C, Mione M, Hurlstone A, Martin P. Live imaging of innate immune cell sensing of transformed cells in zebrafish larvae: parallels between tumor initiation and wound inflammation. PLoS Biology 2010;8(12) e1000562.
Grabher C, Cliffe A, Miura K, Hayflick J, Pepperkock R, Rorth P, et al. Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka. Journal of Leukocyte Biology 2007;81263-71.
Mathias JR, Dodd ME, Walters KB, Yoo SK, Ranheim EA, Huttenlocher A. Characterization of zebrafish larval inflammatory macrophages. Developmental and Comparative Immunology 2009;33(11) 1212-7.
Fixe P, Praloran V. Macrophage colony-stimulating-factor (M-CSF or CSF-1) and its receptor: structure-function relationships. European Cytokine Network 1997;8(2) 125-36.
Stanley ER, Berg KL, Einstein DB, Lee PS, Pixley FJ, Wang Y, et al. Biology and action of colony--stimulating factor-1. Molecular Reproduction and Development 1997;46(1) 4-10.
Guilbert LJ, Stanley ER. Specific interaction of murine colony-stimulating factor with mononuclear phagocytic cells. Journal of Cell Biology 1980;85(1) 153-9.
Tushinski RJ, Oliver IT, Guilbert LJ, Tynan PW, Warner JR, Stanley ER. Survival of mononuclear phagocytes depends on a lineage-specific growth factor that the differentiated cells selectively destroy. Cell 1982;28(1) 71-81.
Ladner MB, Martin GA, Noble JA, Nikoloff DM, Tal R, Kawasaki ES, et al. Human CSF-1: gene structure and alternative splicing of mRNA precursors. EMBO Journal 1987;6(9) 2693-8.
Kawasaki ES, Ladner MB. Molecular biology of macrophage colony-stimulating factor. Immunology Series 1990;49155-76.
Pandit J, Bohm A, Jancarik J, Halenbeck R, Koths K, Kim SH. Three-dimensional structure of dimeric human recombinant macrophage colony-stimulating factor. Science 1992;258(5086) 1358-62.
Praloran V, Gascan H, Papin S, Chevalier S, Trossaert M, Boursier MC. Inducible production of macrophage colony-stimulating factor (CSF-1) by malignant and normal human T cells. Leukemia 1990;4(6) 411-4.
Fretier S, Besse A, Delwail A, Garcia M, Morel F, Leprivey-Lorgeot V, et al. Cyclosporin A inhibition of macrophage colony-stimulating factor (M-CSF) production by activated human T lymphocytes. Journal of Leukocyte Biology 2002;71(2) 289-94.
Hallet MM, Praloran V, Vie H, Peyrat MA, Wong G, Witek-Giannotti J, et al. Macrophage colony-stimulating factor (CSF-1) gene expression in human T-lymphocyte clones. Blood 1991;77(4) 780-6.
Horiguchi J, Warren MK, Kufe D. Expression of the macrophage-specific colony-stimulating factor in human monocytes treated with granulocyte-macrophage colony-stimulating factor. Blood 1987;69(4) 1259-61.
Oster W, Lindemann A, Horn S, Mertelsmann R, Herrmann F. Tumor necrosis factor (TNF)-alpha but not TNF-beta induces secretion of colony stimulating factor for macrophages (CSF-1) by human monocytes. Blood 1987;70(5) 1700-3.
Lin H, Lee E, Hestir K, Leo C, Huang M, Bosch E, et al. Discovery of a cytokine and its receptor by functional screening of the extracellular proteome. Science 2008;320(5877) 807-11.
Wei S, Nandi S, Chitu V, Yeung YG, Yu W, Huang M, et al. Functional overlap but differential expression of CSF-1 and IL-34 in their CSF-1 receptor-mediated regulation of myeloid cells. Journal of Leukocyte Biology 2010;88(3) 495-505.
Chihara T, Suzu S, Hassan R, Chutiwitoonchai N, Hiyoshi M, Motoyoshi K, et al. IL-34 and M-CSF share the receptor Fms but are not identical in biological activity and signal activation. Cell Death and Differentiation 2010;17(12) 1917-27.
Garceau V, Smith J, Paton IR, Davey M, Fares MA, Sester DP, et al. Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products. Journal of Leukocyte Biology 2010;87(5) 753-64.
Rettenmier CW, Chen JH, Roussel MF, Sherr CJ. The product of the c-fms proto-oncogene: a glycoprotein with associated tyrosine kinase activity. Science 1985;228(4697) 320-2.
Sherr CJ, Rettenmier CW, Sacca R, Roussel MF, Look AT, Stanley ER. The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF-1. Cell 1985;41(3) 665-76.
Pixley FJ, Stanley ER. CSF-1 regulation of the wandering macrophage: complexity in action. Trends in Cell Biology 2004;14(11) 628-38.
Chen X, Liu H, Focia PJ, Shim AH, He X. Structure of macrophage colony stimulating factor bound to FMS: diverse signaling assemblies of class III receptor tyrosine kinases. Proceedings of the National Academy of Science U S A 2008;105(47) 18267-72.
Hamilton JA. CSF-1 signal transduction. Journal of Leukocyte Biology 1997;62(2) 145-55.
Yoshida H, Hayashi S, Kunisada T, Ogawa M, Nishikawa S, Okamura H, et al. The murine mutation osteopetrosis is in the coding region of the macrophage colony stimulating factor gene. Letters to Nature 1990;345442-4.
Marks SC, Jr., Lane PW. Osteopetrosis, a new recessive skeletal mutation on chromosome 12 of the mouse. Journal of Heredity 1976;67(1) 11-8.
Wiktor-Jedrzejczak W, Bartocci A, Ferrante AW, Jr., Ahmed-Ansari A, Sell KW, Pollard JW, et al. Total absence of colony-stimulating factor 1 in the macrophage-deficient osteopetrotic (op/op) mouse. Proceedings of the National Academy of Science U S A 1990;87(12) 4828-32.
Pollard JW, Stanley ER. Pleiotropic roles for CSF-1 in development defined by the mouse mutation osteopetrotic. Advances in Developmental Biochemistry 1996;4153-93.
Dai XM, Ryan GR, Hapel AJ, Dominguez MG, Russell RG, Kapp S, et al. Targeted disruption of the mouse colony-stimulating factor 1 receptor gene results in osteopetrosis, mononuclear phagocyte deficiency, increased primitive progenitor cell frequencies, and reproductive defects. Blood 2002;99(1) 111-20.
Hanington PC, Wang T, Secombes CJ, Belosevic M. Growth factors of lower vertebrates: characterization of goldfish ( Carassius auratusL.) macrophage colony-stimulating factor-1. Journal of Biological Chemistry 2007;282(44) 31865-72.
Hanington PC, Hitchen SJ, Beamish LA, Belosevic M. Macrophage colony stimulating factor (CSF-1) is a central growth factor of goldfish macrophages. Fish and Shellfish Immunology 2009;26(1) 1-9.
Grayfer L, Hanington PC, Belosevic M. Macrophage colony-stimulating factor (CSF-1) induces pro-inflammatory gene expression and enhances antimicrobial responses of goldfish ( Carassius auratusL.) macrophages. Fish and Shellfish Immunology 2009;26(3) 406-13.
Wang T, Hanington PC, Belosevic M, Secombes CJ. Two macrophage colony-stimulating factor genes exist in fish that differ in gene organization and are differentially expressed. Journal of Immunology 2008;181(5) 3310-22.
How GF, Venkatesh B, Brenner S. Conserved linkage between the puffer fish ( Fugu rubripes) and human genes for platelet-derived growth factor receptor and macrophage colony-stimulating factor receptor. Genome Research 1996;6(12) 1185-91.
Williams H, Brenner S, Venkatesh B. Identification and analysis of additional copies of the platelet-derived growth factor receptor and colony stimulating factor 1 receptor genes in fugu. Gene 2002;295(2) 255-64.
Herbomel P, Thisse B, Thisse C. Zebrafish early macrophages colonize cephalic mesenchyme and developing brain, retina, and epidermis through a M-CSF receptor-dependent invasive process. Developmental Biology 2001;238(2) 274-88.
Honda T, Nishizawa T, Uenobe M, Kohchi C, Kuroda A, Ototake M, et al. Molecular cloning and expression analysis of a macrophage-colony stimulating factor receptor-like gene from rainbow trout, Oncorhynchus mykiss. Molecular Immunology 2005;42(1) 1-8.
Roca FJ, Sepulcre MA, Lopez-Castejon G, Meseguer J, Mulero V. The colony-stimulating factor-1 receptor is a specific marker of macrophages from the bony fish gilthead seabream. Molecular Immunology 2006;43(9) 1418-23.
Mulero I, Pilar Sepulcre M, Roca FJ, Meseguer J, Garcia-Ayala A, Mulero V. Characterization of macrophages from the bony fish gilthead seabream using an antibody against the macrophage colony-stimulating factor receptor. Developmental and Comparative Immunology 2008;32(10) 1151-9.
Braasch I, Salzburger W, Meyer A. Asymmetric evolution in two fish-specifically duplicated receptor tyrosine kinase paralogons involved in teleost coloration. Molecular Biology and Evolution 2006;23(6) 1192-202.
Parichy DM, Ransom DG, Paw B, Zon LI, Johnson SL. An orthologue of the kit-related gene fms is required for development of neural crest-derived xanthophores and a subpopulation of adult melanocytes in the zebrafish, Danio rerio. Development 2000;127(14) 3031-44.
Parichy DM, Turner JM. Temporal and cellular requirements for Fms signaling during zebrafish adult pigment pattern development. Development 2003;130(5) 817-33.
Theilgaard-Monch K, Jacobsen LC, Borup R, Rasmussen T, Bjerregaard MD, Nielsen FC, et al. The transcriptional program of terminal granulocytic differentiation. Blood 2005;105(4) 1785-96.
Meseguer J, Esteban MA, Garcia Ayala A, Lopez Ruiz A, Agulleiro B. Granulopoiesis in the head-kidney of the sea bass ( Dicentrarchus labraxL.): an ultrastructural study. Archives of Histology and Cytology 1990;53(3) 287-96.
Yoo SK, Huttenlocher A. Spatiotemporal photolabeling of neutrophil trafficking during inflammation in live zebrafish. Journal of Leukocyte Biology 2011;89(5) 661-7.
Harun NO, Zou J, Zhang YA, Nie P, Secombes CJ. The biological effects of rainbow trout ( Oncorhynchus mykiss) recombinant interleukin-8. Developmental and Comparative Immunology 2008;32(6) 673-81.
Overland HS, Pettersen EF, Ronneseth A, Wergeland HI. Phagocytosis by B-cells and neutrophils in Atlantic salmon ( Salmo salar L.) and Atlantic cod ( Gadus morhuaL.). Fish and Shellfish Immunology 2010;28(1) 193-204.
Mayumi M, Takeda Y, Hoshiko M, Serada K, Murata M, Moritomo T, et al. Characterization of teleost phagocyte NADPH oxidase: molecular cloning and expression analysis of carp ( Cyprinus carpio) phagocyte NADPH oxidase. Molecular Immunology 2008;45(6) 1720-31.
Palic D, Andreasen CB, Menzel BW, Roth JA. A rapid, direct assay to measure degranulation of primary granules in neutrophils from kidney of fathead minnow ( Pimephales promelasRafinesque, 1820). Fish and Shellfish Immunology 2005;19(3) 217-27.
Palic D, Ostojic J, Andreasen CB, Roth JA. Fish cast NETs: neutrophil extracellular traps are released from fish neutrophils. Developmental and Comparative Immunology 2007;31(8) 805-16.
Palic D, Andreasen CB, Ostojic J, Tell RM, Roth JA. Zebrafish ( Danio rerio) whole kidney assays to measure neutrophil extracellular trap release and degranulation of primary granules. Journal of Immunological Methods 2007;319(1-2) 87-97.
Mantovani A, Cassatella MA, Costantini C, Jaillon S. Neutrophils in the activation and regulation of innate and adaptive immunity. Nature Reviews Immunology 2011;11(8) 519-31.
Kumar V, Sharma A. Neutrophils: Cinderella of innate immune system. International Immunopharmacology 2010;10(11) 1325-34.
Panopoulos AD, Watowich SS. Granulocyte colony-stimulating factor: molecular mechanisms of action during steady state and 'emergency' hematopoiesis. Cytokine 2008;42(3) 277-88.
Demetri GD, Griffin JD. Granulocyte colony-stimulating factor and its receptor. Blood 1991;78(11) 2791-808.
Watari K, Asano S, Shirafuji N, Kodo H, Ozawa K, Takaku F, et al. Serum granulocyte colony-stimulating factor levels in healthy volunteers and patients with various disorders as estimated by enzyme immunoassay. Blood 1989;73(1) 117-22.
Kawakami M, Tsutsumi H, Kumakawa T, Abe H, Hirai M, Kurosawa S, et al. Levels of serum granulocyte colony-stimulating factor in patients with infections. Blood 1990;76(10) 1962-4.
Cheers C, Haigh AM, Kelso A, Metcalf D, Stanley ER, Young AM. Production of colony-stimulating factors (CSFs) during infection: separate determinations of macrophage-, granulocyte-, granulocyte-macrophage-, and multi-CSFs. Infection and Immunity 1988;56(1) 247-51.
Avalos BR. Molecular analysis of the granulocyte colony-stimulating factor receptor. Blood 1996;88(3) 761-77.
Shimoda K, Okamura S, Harada N, Niho Y. Detection of the granulocyte colony-stimulating factor receptor using biotinylated granulocyte colony-stimulating factor: presence of granulocyte colony-stimulating factor receptor on CD34-positive hematopoietic progenitor cells. Research in Experimental Medicine (Berl) 1992;192(4) 245-55.
Liongue C, Wright C, Russell AP, Ward AC. Granulocyte colony-stimulating factor receptor: stimulating granulopoiesis and much more. International Journal of Biochemistry and Cell Biology 2009;41(12) 2372-5.
Nicola NA, Metcalf D. Binding of 125I-labeled granulocyte colony-stimulating factor to normal murine hemopoietic cells. Journal of Cellular Physiology 1985;124(2) 313-21.
Aritomi M, Kunishima N, Okamoto T, Kuroki R, Ota Y, Morikawa K. Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme. Nature 1999;401(6754) 713-7.
Tamada T, Honjo E, Maeda Y, Okamoto T, Ishibashi M, Tokunaga M, et al. Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex. Proceedings of the National Academy of Science U S A 2006;103(9) 3135-40.
Honjo E, Tamada T, Maeda Y, Koshiba T, Matsukura Y, Okamoto T, et al. Crystallization of a 2:2 complex of granulocyte-colony stimulating factor (GCSF) with the ligand-binding region of the GCSF receptor. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 2005;61(Pt 8) 788-90.
Richards MK, Liu F, Iwasaki H, Akashi K, Link DC. Pivotal role of granulocyte colony-stimulating factor in the development of progenitors in the common myeloid pathway. Blood 2003;102(10) 3562-8.
Piper MG, Massullo PR, Loveland M, Druhan LJ, Kindwall-Keller TL, Ai J, et al. Neutrophil elastase downmodulates native G-CSFR expression and granulocyte-macrophage colony formation. Journal of Inflammation (Lond) 2010;7(1) 5.
Lieschke GJ, Grail D, Hodgson G, Metcalf D, Stanley E, Cheers C, et al. Mice lacking granulocyte colony-stimulating factor have chronic neutropenia, granulocyte and macrophage progenitor cell deficiency, and impaired neutrophil mobilization. Blood 1994;84(6) 1737-46.
Zhan Y, Lieschke GJ, Grail D, Dunn AR, Cheers C. Essential roles for granulocyte-macrophage colony-stimulating factor (GM-CSF) and G-CSF in the sustained hematopoietic response of Listeria monocytogenes-infected mice. Blood 1998;91(3) 863-9.
Liu F, Wu HY, Wesselschmidt R, Kornaga T, Link DC. Impaired production and increased apoptosis of neutrophils in granulocyte colony-stimulating factor receptor-deficient mice. Immunity 1996;5(5) 491-501.
Tsuchiya M, Asano S, Kaziro Y, Nagata S. Isolation and characterization of the cDNA for murine granulocyte colony-stimulating factor. Proceedings of the National Academy of Science U S A 1986;83(20) 7633-7.
Hubel K, Dale DC, Liles WC. Therapeutic use of cytokines to modulate phagocyte function for the treatment of infectious diseases: current status of granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, macrophage colony-stimulating factor, and interferon-gamma. Journal of Infectious Diseases 2002;185(10) 1490-501.
Greenbaum AM, Link DC. Mechanisms of G-CSF-mediated hematopoietic stem and progenitor mobilization. Leukemia 2011;25(2) 211-7.
Santos MD, Yasuike M, Hirono I, Aoki T. The granulocyte colony-stimulating factors (CSF3s) of fish and chicken. Immunogenetics 2006;58(5-6) 422-32.
Nam BH, An GH, Baeck GW, Kim MC, Kim JW, Park HJ, et al. Molecular cloning and expression of cDNAs for two distinct granulocyte colony stimulating factor genes from black rockfish Sebastes schlegelii. Fish and Shellfish Immunology 2009;27(2) 360-4.
Liongue C, Hall CJ, O'Connell BA, Crosier P, Ward AC. Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration. Blood 2009;113(11) 2535-46.
Katzenback BA, Belosevic M. Characterization of granulocyte colony stimulating factor receptor of the goldfish ( Carassius auratusL.). Developmental and Comparative Immunology 2012;36(1) 199-207.
Tenen DG, Hromas R, Licht JD, Zhang DE. Transcription factors, normal myeloid development, and leukemia. Blood 1997;90(2) 489-519.
Milbrandt J. A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor. Science 1987;238(4828) 797-9.
Sukhatme VP, Kartha S, Toback FG, Taub R, Hoover RG, Tsai-Morris CH. A novel early growth response gene rapidly induced by fibroblast, epithelial cell and lymphocyte mitogens. Oncogene Research 1987;1(4) 343-55.
Chavrier P, Zerial M, Lemaire P, Almendral J, Bravo R, Charnay P. A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells. EMBO Journal 1988;7(1) 29-35.
Patwardhan S, Gashler A, Siegel MG, Chang LC, Joseph LJ, Shows TB, et al. EGR3, a novel member of the Egr family of genes encoding immediate-early transcription factors. Oncogene 1991;6(6) 917-28.
Crosby SD, Puetz JJ, Simburger KS, Fahrner TJ, Milbrandt J. The early response gene NGFI-C encodes a zinc finger transcriptional activator and is a member of the GCGGGGGCG (GSG) element-binding protein family. Molecular and Cellular Biology 1991;11(8) 3835-41.
Thiel G, Cibelli G. Regulation of life and death by the zinc finger transcription factor Egr-1. Journal of Cellular Physiology 2002;193(3) 287-92.
Krishnaraju K, Nguyen HQ, Liebermann DA, Hoffman B. The zinc finger transcription factor Egr-1 potentiates macrophage differentiation of hematopoietic cells. Molecular and Cellular Biology 1995;15(10) 5499-507.
Krishnaraju K, Hoffman B, Liebermann DA. Early growth response gene 1 stimulates development of hematopoietic progenitor cells along the macrophage lineage at the expense of the granulocyte and erythroid lineages. Blood 2001;97(5) 1298-305.
Nguyen HQ, Hoffman-Liebermann B, Liebermann DA. The zinc finger transcription factor Egr-1 is essential for and restricts differentiation along the macrophage lineage. Cell 1993;72(2) 197-209.
Krishnaraju K, Hoffman B, Liebermann DA. The zinc finger transcription factor Egr-1 activates macrophage differentiation in M1 myeloblastic leukemia cells. Blood 1998;92(6) 1957-66.
Carter JH, Tourtellotte WG. Early growth response transcriptional regulators are dispensable for macrophage differentiation. Journal of Immunology 2007;178(5) 3038-47.
Lee SL, Tourtellotte LC, Wesselschmidt RL, Milbrandt J. Growth and differentiation proceeds normally in cells deficient in the immediate early gene NGFI-A. Journal of Biological Chemistry 1995;270(17) 9971-7.
Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, et al. Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells. Cell Death and Differentiation 2006;13(10) 1686-96.
Drummond IA, Rohwer-Nutter P, Sukhatme VP. The zebrafish egr1 gene encodes a highly conserved, zinc-finger transcriptional regulator. DNA and Cell Biology 1994;13(10) 1047-55.
van der Meer LT, Jansen JH, van der Reijden BA. Gfi1 and Gfi1b: key regulators of hematopoiesis. Leukemia 2010;24(11) 1834-43.
Karsunky H, Zeng H, Schmidt T, Zevnik B, Kluge R, Schmid KW, et al. Inflammatory reactions and severe neutropenia in mice lacking the transcriptional repressor Gfi1. Nature Genetics 2002;30(3) 295-300.
Hock H, Hamblen MJ, Rooke HM, Traver D, Bronson RT, Cameron S, et al. Intrinsic requirement for zinc finger transcription factor Gfi-1 in neutrophil differentiation. Immunity 2003;18(1) 109-20.
Zarebski A, Velu CS, Baktula AM, Bourdeau T, Horman SR, Basu S, et al. Mutations in growth factor independent-1 associated with human neutropenia block murine granulopoiesis through colony stimulating factor-1. Immunity 2008;28(3) 370-80.
de la Luz Sierra M, Sakakibara S, Gasperini P, Salvucci O, Jiang K, McCormick PJ, et al. The transcription factor Gfi1 regulates G-CSF signaling and neutrophil development through the Ras activator RasGRP1. Blood 2010;115(19) 3970-9.
Dufourcq P, Rastegar S, Strahle U, Blader P. Parapineal specific expression of gfi1 in the zebrafish epithalamus. Gene Expression Patterns 2004;4(1) 53-7.
Wei W, Wen L, Huang P, Zhang Z, Chen Y, Xiao A, et al. Gfi1.1 regulates hematopoietic lineage differentiation during zebrafish embryogenesis. Cell Research 2008;18(6) 677-85.
Paun A, Pitha PM. The IRF family, revisited. Biochimie 2007;89(6-7) 744-53.
Wang H, Morse HC, 3rd. IRF8 regulates myeloid and B lymphoid lineage diversification. Immunology Research 2009;43(1-3) 109-17.
Holtschke T, Lohler J, Kanno Y, Fehr T, Giese N, Rosenbauer F, et al. Immunodeficiency and chronic myelogenous leukemia-like syndrome in mice with a targeted mutation of the ICSBP gene. Cell 1996;87(2) 307-17.
Turcotte K, Gauthier S, Tuite A, Mullick A, Malo D, Gros P. A mutation in the Icsbp1 gene causes susceptibility to infection and a chronic myeloid leukemia-like syndrome in BXH-2 mice. Journal of Experimental Medicine 2005;201(6) 881-90.
Tamura T, Nagamura-Inoue T, Shmeltzer Z, Kuwata T, Ozato K. ICSBP directs bipotential myeloid progenitor cells to differentiate into mature macrophages. Immunity 2000;13(2) 155-65.
Holland JW, Karim A, Wang T, Alnabulsi A, Scott J, Collet B, et al. Molecular cloning and characterization of interferon regulatory factors 4 and 8 (IRF-4 and IRF-8) in rainbow trout, Oncorhynchus mykiss. Fish and Shellfish Immunology 2010;29(1) 157-66.
Li L, Jin H, Xu J, Shi Y, Wen Z. Irf8 regulates macrophage versus neutrophil fate during zebrafish primitive myelopoiesis. Blood 2011;117(4) 1359-69.
Eichmann A, Grapin-Botton A, Kelly L, Graf T, Le Douarin NM, Sieweke M. The expression pattern of the mafB/kr gene in birds and mice reveals that the kreisler phenotype does not represent a null mutant. Mechanisms of Development 1997;65(1-2) 111-22.
Kelly LM, Englmeier U, Lafon I, Sieweke MH, Graf T. MafB is an inducer of monocytic differentiation. EMBO Journal 2000;19(9) 1987-97.
Aziz A, Soucie E, Sarrazin S, Sieweke MH. MafB/c-Maf deficiency enables self-renewal of differentiated functional macrophages. Science 2009;326(5954) 867-71.
Nobrega MA, Pennacchio LA. Comparative genomic analysis as a tool for biological discovery. Journal of Physiology 2004;554(Pt 1) 31-9.
Williams H, Brenner S, Venkatesh B. Characterization of the platelet-derived growth factor receptor alpha and c-kit genes in the pufferfish Fugu rubripes. DNA Sequence 2002;13(5) 263-70.
Liu L, Korzh V, Balasubramaniyan NV, Ekker M, Ge R. Platelet-derived growth factor A (pdgf-a) expression during zebrafish embryonic development. Developmental Genes and Evolution 2002;212(6) 298-301.
Taylor JS, Van de Peer Y, Braasch I, Meyer A. Comparative genomics provides evidence for an ancient genome duplication event in fish. Philosophical Transactions of the Royal Society of London B: Biological Sciences 2001;356(1414) 1661-79.
Ravi V, Venkatesh B. Rapidly evolving fish genomes and teleost diversity. Current Opinion in Genetics and Development 2008;18(6) 544-50.