Major landmarks in soybean organogenesis and transformation
Soybean is now an essential and dominant source of protein and oil with numerous uses in feed, food and industrial applications. It is the world’s primary source of vegetable oil and protein feed supplement for livestock. The global production of soybeans is 250-260 million tons per year. The US is the largest producer with 90.6 million metric tons. Other major countries such as Brazil, Argentina, China and India contributing 70, 49.5, 15.2 and 9.6 million metric tons, respectively . The US, Brazil and Argentina are the major exporters of beans; while China and Europe are the major importers. The annual world market value is around 2 billion US dollars, which stands second in world food production.
Recent nutritional studies claim that consumption of soybean reduces cancer, blood serum cholesterol, osteoporosis and heart diseases . This has sparked increased demand for the many edible soybean products. The priority for more meat in diets among the world’s population has also increased the demand for soybean protein for livestock and poultry feed.
Soybean seeds are comprised of 40% protein, mostly consisting of the globulins β-conglycinin (7S globulin) and glycinin (11S globulin). The oil portion of the seed is composed primarily of five fatty acids. Palmitic and stearic acids are saturated fatty acids and comprise 15% of the oil. Soybean is rich in the unsaturated fatty acids like oleic, linoleic and linolenic, which make up 85% of the oil. Soybeans are a good source of minerals, B vitamins, folic acid and isoflavones, which are credited with slowing cancer development, heart diseases and osteoporosis .
The productivity of soybean has been limited due to their susceptibility to pathogens and pests, sensitivity to environmental stresses, poor pollination and low harvest index. Among the abiotic stresses, drought is considered the most devastating, commonly reducing soybean yield by approximately 40% and affecting all stages of plant growth and development; from germination to flowering, and seed filling and development as well as seed quality . It suffers from many kinds of fungal diseases, such as frogeye leaf spot and brown spot . As demand increases for soybean oil and protein, the improvement of soybean quality and production through genetic transformation and functional genomics becomes an important issue throughout the world .
The main objectives of soybean improvement include increase in yield, development of resistance to various insects, diseases and nutritional quality. Commercial breeding is still very important for the genetic improvement of the crop. However, breeding is difficult due to the fact that the soybean is a self pollinating crop, and the genetic base of modern soybean cultivars is quite narrow . Most of the current soybean genotypes have been derived from common ancestors; therefore, conventional breeding strategies are limited in capability to expand the soybean genetic base. Recent advances in
Although numerous methods have been developed for introducing genes into plant genomes, the transformation efficiency for soybean still remains low . Since the first successful transformation of soybean was reported , two major methods have been used in soybean transformation: one is particle bombardment of embryogenic tissue and another is
The present review gives an overview on the problems associated with low transformation efficiency, and the research conducted to improve tissue culture and transformation efficiency of soybean during the past (Table 1&2) and also discuss the future prospects, demands of these technologies and upcoming new technologies in soybean improvement.
|1973||Hypocotyl||Adventitious bud development||Kimball and Bingham, |
|1980||Cotyledonary node||Shoot morphogenesis||Cheng et al. |
|1986||Immature embryo||Plant regeneration from callus||Barwale et al. |
|1986||Cotyledonary node||Multiple shoot formation||Barwale et al. |
|1986||Cotyledonary node||Multiple shoot formation||Wright et al. |
|1987||Epicotyl||Callus induction and shoot regeneration||Wright et al. |
||Hinchee et al. |
|1988||Immature seeds||Developed transgenic soybean by Particle bombardment||McCabe et al. |
||Chee et al. |
|1989||Immature seed||Particle bombardment of meristems||Christou et al. |
|1990||Immature cotyledon||Plant regeneration from protoplast||Luo et al. |
|1990||Cotyledon, cotyledonary node||Evaluated
||Delzer et al. |
|1990||Immature cotyledon, plumule, cotyledonary node||Analysed plant regeneration efficiency of various explants||Yang et al. |
|1990||Immature embryo||Organogenesis and plant regeneration||Yeh,|
|1990||Primary leaf node||Adventitious shoot formation||Kim et al. |
|1991||Immature cotyledon||Plant regeneration from protoplast||Dhir et al. |
|1992||Epicotyl and hypocotyl||Investigated the stimulative effect of allantoin and amides on shoot regeneration||Shetty, et al. |
||Sato et al. |
|1994||Primary leaf node||Investigated the synergistic effect of proline and micronutrients on shoot regeneration||Kim et al. |
|1996||Cotyledonary node||Developed transgenic soybean resistance to bean pod mottle virus (BPMV)||Di et al. |
|1997||Cotyledonary node and hypocotyl||Multiple shoot induction by TDZ||Kaneda et al. |
|1998||Cotyledonary node||Evaluation of sonication assisted Agrobacterium mediated
transformation (SAAT) for cotyledonary node
|Meurer et al. |
|1998||Hypocotyl||Adventitious shoot regeneration||Dan and Reichert, |
|1999||Cotyledonary node||Assessed the use of glufosinate as a selective agent in
||Zhang et al. |
||Xing et al. |
|2000||Cotyledonary node||Evaluated the effect of glyphosate as a selective agent for
||Clemente et al. |
|2000||Embryonic axes||Used of Imazapyr as selection agent for selection of meristematic soybean cells||Aragao et al. |
|2001||Cotyledonary node||Investigated the use of thiol compound to increase transformation frequency||Olhoft et al. |
||Olhoft and Somers, |
|2001||Cotyledonary node||Developed transgenic soybean plants resistant to soybean mosaic virus (SMV)||Wang et al. |
|2001||Cotyledonary node||Expressed oxalate oxidase gene for resistant to sclerotinia stem rot caused by
||Donaldson et al. |
|2003||Hypocotyl||Screened soybean genotype for adventitious organogenic regeneration||Reichert et al. |
|2003||Cotyledonary node||Assessed the effect of genotype, plant growth regulators and sugars on regeneration from calli||Sairam et al. |
|2003||Cotyledonary node||Used mixture of thiol compounds and hygromycin based selection for increased transformation efficiency||Olhoft et al. |
|2004||Cotyledonary node||Assessed glufosinate selection for increased transformation efficiency||Zeng et al. |
|2004||Cotyledonary node||Investigated the effect of seed vigor of explant source, selection agent and antioxidant on
||Paz et al. |
|2004||Cotyledonary node||Transferred chitinase gene and the barley ribosome-inactivating protein gene to enhance fungal resistance||Li et al. |
|2004||Mature and immature cotyledon||Shoot regeneration||Franklin et al. |
|2004||Embryonic tip||Established regeneration and
||Liu et al. |
|2004||Cotyledonary node||Established liquid medium based system for selection transformed plants||Yun, |
|2005||Cotyledonary node||Developed repetitive organogenesis system||Shan et al. |
|2005||Cotyledonary node||Expressed Escherichia coli K99 fimbriae
subunit antigen in soybean to use as edible vaccine
|Piller et al. |
||Paz et al. |
||Olhoft et al. |
|2007||Cotyledonary node||Expressed synthetic
||Miklos et al. |
|2007||Cotyledonary node and leaf node||Established organogenic callus induction and
||Hong et al ., |
|2007||Half seed||Expressed jasmonic acid carboxyl methyltransferase in soybean to produce methyl jasmonate, which resulted in tolerant to water stress||Xue et al. |
|2008||Hypocotyl||Used silver nitrate to enhance adventitious shoot regeneration after
||Wang and Xu, |
|2008||Cotyledonary node||Improved transformation efficiency using surfactant Silwet L-77 during
||Liu et al. |
|2008||Cotyledonary node||Developed rapid regeneration system using whole cotyledonary node||Ma and Wu, |
|2010||Cotyledonary node||Production of isoflavone in callus cell lines by expression of isoflavone synthase gene.||Jiang et al. |
|2010||Cotyledon and embryo||Developed shoot regeneration from calli of soybean cv.Pyramid||Joyner et al. |
|2011||Hypocotyl||Transgenic soybean with low phytate content||Yang et al. |
|2011||Cotyledon||Developed transgenic soybean with increased Vitamin E content by transferring γ-tocopherol methyltransferase (γ-TMT) gene in to seedling cotyledon||Lee et al. |
|Embryonic axes||1983||Embryoids development and plant regeneration
||Christianson et al.|
|Immature cotyledon||1984||Somatic embryo Induction||Lippmann & Lippmann, |
|Immature cotyledon||1985||Plant regeneration
||Lazzeri et al. |
|Immature embryo||1985||Somatic embryogenesis and assessment of genotypic variation||Ranch et al. |
|Immature embryo, cotyledon and, hypocotyl from germinating seedling||1986||Somatic embryogenesis from callus||Ghazi et al. |
|Hypocotyl and cotyledon||1986||Embryoids development in suspension culture||Kerns et al. |
|Immature embryo and cotyledon||1987||Investigated the effect of nutritional, physical, and chemical factors on somatic embryogenesis||Lazzeri et al. |
|Immature cotyledon||1988||Investigated the effect of auxin and orientation of explant on somatic embryogenesis||Hartweck et al. |
|Immature cotyledon||1988||Analysed genotype dependency and High concentration of auxin on somatic embryo induction||Komatsuda and Ohyama, |
|Immature cotyledon||1988||Investigated the interaction between auxin and sucrose during somatic embryogenesis||Lazzeri et al. |
|Immature cotyledon||1988||Germination frequency of somatic embryo has been improved by reducing the exposure to auxin||Parrott et al. |
|Immature cotyledon||1988||Developed rapid growing maintainable embryogenic suspension culture||Finer and Nagasawa, |
|Immature cotyledon||1988||Histological analysis to investigate secondary somatic embryo formation.||Finer, |
|Immature cotyledon||1989||Demonstrated the effect of genotype on embryogenesis||Parrott et al. |
|Immature cotyledon||1989||Developed primary transformants expressing zein gene by agrobacterium mediated transformation||Parrott et al. |
|Immature cotyledon||1989||Assayed somatic embryo maturation for conversion into plantlets||Buchheim et al. |
|Immature cotyledon||1989||Investigated the developmental aspects of somatic embryogenesis||Christou and Yang, |
|Immature cotyledon||1990||Screened soybean genotypes for somatic embryo production||Komatsuda et al. |
|Immature cotyledon||1991||Transformed embryogenic cultures with
||Finer and McMullen., |
|Immature cotyledon||1991||Analysed the interaction between genotype and sucrose concentration on somatic embryogenesis||Komatsuda et al. |
|Immature cotyledon||1991||Demonstrated adventitious shoot formation from cotyledonary and torpedo stage embryo||Wright et al. |
|Immature cotyledon||1992||Somatic embryo proliferation by somatic embryo cycling.||Liu et al. |
|Immature cotyledon||1993||Improved germination efficiency of somatic embryos of cultivar H7190 by desiccation||Bailey et al. |
|Immature cotyledon||1993||Demonstrated genotypic effect on induction, proliferation, maturation and germination of somatic embryo||Bailey et al. |
|Immature cotyledon||1993||Investigated the factors affecting somatic embryogenesis||Lippmann & Lippmann, |
|Immature cotyledon||1993||Soybean transformation by particle bombardment of embryogenic cultures||Sato et al. |
|Immature cotyledon||1994||Developed transgenic soybean resistance to insect.||Parrott et al. |
|Immature embryos||1995||Investigated the effect of glutamine and sucrose on dry matter accumulation and composition of somatic embryo.||Saravitz and Raper, |
|Immature cotyledon||1996||Demonstrated the significance of embryo cycling for transformation||Liu et al.|
|Immature cotyledon||1996||Transformed embryogenic cultures with 12 different plansmid
||Hadi et al. |
|Immature cotyledon||1996||Developed transgenic soybean expressing a synthetic Bacillus thuringiensis insecticidal crystal protein gene (BtcrylAc) which is resistance to insects||Stewart et al. |
|Immature cotyledon||1997||Investigated the effect of ethylene inhibitors on embryo histodifferentiation and maturation||Santos et al. |
|Epicotyls and primary leaves||1997||Somatic embryogenesis and plant regeneration from cotyledon, epicotyls and primary leaves||Rajasekaran and Pello, |
|Immature cotyledon||1997||Studied the effect of explant orientiation, pH, solidifying agent and wounding on induction of soybean from immature cotyledons||Santarém et al. |
|Immature cotyledon||1998||Studied growth characteristics of embryogenic cultures for transformability||Hazal et al. |
|Immature cotyledon||1998||Established sonication-assisted A
||Santarem et al.|
|Immature cotyledon||1998||Established sonication-assisted Agrobacterium mediated transformation of embryogenic suspension culture tissue||Trick and Finer, |
|Immature cotyledon||1998||Improved proliferation efficiency of embryogenic cultures by modifying sucrose and nitrogen content in medium||Samoylov et al. |
|Immature cotyledon||1998||Developed liquid medium based system for histodifferentiation of embryogenic cultures||Samoylov et al. |
|Immature cotyledon||1998||Studied soluble carbohydrate content in soybean somatic and zygotic embryo during development.||Chanprame et al. |
|Immature cotyledon||1999||Studied the factors influencing transformation of prolific embryogenic cultures using bombardment||Santarem and Finer, |
|Immature cotyledons||1999||Developed transgenic plants with bovine milk protein, β-casein||Maughan et al. |
|Immature cotyledons||1999||Transformed GFP into embryogenic suspension culture with the aim to improve transformation and regeneration strategy||Ponappa et al. |
|Immature cotyledons||2000||Improved somatic embryo development and maturation by application of ABA||Tian and Brown, |
|Immature cotyledon||2000||Screened genotypes for proliferative embryogenesis||Simmonds and Donaldson, |
|Immature cotyledons||2000||Studied physical factors influencing somatic embryo development from immature cotyledons.||Bonacin et al. |
|Immature cotyledon||2000||Investigated the factors affecting
||Yan et al. |
|Immature cotyledon||1989||Investigated maturation of somatic embryo for efficient conversion into plantlets||Buchheim et al. |
|Immature cotyledon||2000||Developed and evaluated transgenic soybean expressing a synthetic cry1Ac gene from
||Walker et al. |
|Immature cotyledon||2001||Effect of polyethylene glycol and sugar alcohols on soybean somatic embryo germination and conversion||Walker and Parrott, |
|Immature cotyledon||2000||Developed integrated bombardment and
||Droste et al.
|Immature cotyledon||2001||Screened soybean from different location in the US for uniform embryogenic response||Meurer et al. |
|Immature cotyledon||2001||Studied the effect of osmotica for their influence on embryo maturation and germination||Walker & Parrott, |
|Immature cotyledon||2001||Developed transgenic plant expressing 15-kD zein protein under β-phaseolin seed specific promoter||Dinkins et al. |
|Immature cotyledon||2001||Somatic embryogenesis in Brazilian soybean cultivars||Droste et al. |
|Immature cotyledon||2002||Somatic embryogenesis and particle bombardment for south Brazil cultivars||Droste et al. |
|Immature cotyledon||2002||Histological analysis of developmental stages of somatic embryogenesis||Fernando et al. |
|Immature cotyledon||2002||Screened soybean genotypes for somatic embryo induction and maturation capability||Tomlin, |
|Immature cotyledon||2003||Investigated the effect of proliferation, maturation and desiccation on somatic embryo conversion||Moon and Hildebrand, |
|Immature cotyledon||2004||Improved transformation efficiently using
||Ko et al. |
|Immature cotyledon||2004||Developed transgenic plant containing phytase gene that store (produces) more phosphrous in seed.||Chiera et al. |
|Immature cotyledon||2004||Developed fertile transplastomic soybean||Dufourmantel et al.
||Li et al. |
|Immature cotyledon||2004||Improved transformation efficiency using
||Ko and Korban, |
|Immature cotyledon||2004||Analysed media components and pH on somatic embryo induction||Hoffmann et al. |
|Immature cotyledon||2005||Developed transgenic soybean expressing maize γ-zein protein||Li et al. |
|Immature cotyledon||2005||Modified soybean histodifferentiation and msaturation medium with the aim to improve the protein and lipid composition of somatic embryo||Schmidt et al. |
|Immature cotyledon||2005||Analysed the effect of carbon source and polyethylene glycol on embryo conversion||Korbes et al. |
|Immature cotyledon||2006||Improved fatty acid content||Chen et al. |
|Immature cotyledon||2006||Investigated the ontogeny of somatic embryogenesis||Santos et al. |
|Somatic embryo||2006||Developed transgenic soybean resistance to dwarf virus||Tougou et al. |
|Immature cotyledon||2006||Investigated the influence of antibiotics on embryogenic cultures and
||Wiebke et al. |
|Immature cotyledon||2006||Developed transgenic soybean for increased production of ononitol and pinitol||Chiera et al. |
|Immature cotyledon||2007||Developed transgenic soybean resistant to dwarf virus
||Tougou et al. |
|Immature cotyledon||2007||Improved somatic embryogenesis in recalcitrant cultivars by back cross with a highly regenerable cultivar Jack||Kita et al. |
|Immature cotyledon||2007||Evaluated Japanese soybean genotypes for somatic embryogenesis||Hiraga et al. |
|Immature cotyledon||2007||Soybean seed over expressing the
||Tavva et al. |
|Immature cotyledon||2007||Improved protein quality in transgenic soybean transformed with modified Gy1 proglycinin gene with a synthetic DNA encoding four continuous methionines.||EI-Shemy et al. |
|Immature cotyledon||2007||Analysed the effect of Abscisic acid on somatic embryo maturation and conversion.||Weber et al. |
|Immature cotyledon||2007||Developed transgenic soybean resistance to soybean mosaic virus||Furutani et al. |
|Immature cotyledon||2008||Used a new Selectable Marker Gene Conferring resistance to Dinitroanilines||Yemets et al. |
|Immature cotyledon||2008||Developed strategy for transfer of multiple genes
||Schmidt et al. |
|Immature cotyledon||2009||Assessed the effect mannitol, abscisic acid and explant age on somatic embryogenesis in Chinese soybean cultivars||Yang et al. |
|Somatic embryo||2009||Developed transgenic soybean with increased oil content||Rao and Hildebrand, |
|Embryonic tip||2010||somatic embryogenesis and plant regeneration from the immature embryonic shoot tip||Loganathan et al. |
|Immature cotyledon||2010||Developed transgenic soybean with more tryptophan content in seed||Ishimoto et al. |
|Immature cotyledon||2010||Screening of Brazilian soybean genotypes for embryogenesis||Droste et al. |
|Immature cotyledon||2011||Demonstrated Metabolic engineering of soybean seed coat for the production of novel biochemicals||Schnell et al. |
|Immature cotyledon||2011||Investigated developmental profile of storage reserve accumulation in soybean somatic embryos||He et al. |
|Immature cotyledon||2011||Improved transformation efficiency by Micro wounding with DNA free particle bombardment followed by Agrobacterium mediated transformation.||Wiebke et al. |
|Immature cotyledon||2012||Developed vacuum infiltration assisted
||Mariashibu et al. |
2. Organogenesis and transformation
Organogenesis is characterized by the production of a unipolar bud primordium with subsequent development of the primordium into a leafy vegetative shoot. A successful plant regeneration protocol requires appropriate choice of explant, definite media formulations, specific growth regulators, genotype, source of carbohydrate, gelling agent, other physical factors including light regime, temperature, humidity and other factors . Plant regeneration by organogenesis in soybean was first reported by Kimball and Bingham,  from hypocotyl sections followed by Cheng et al. by culturing seedling cotyledonary node segments. Transfer of T-DNA into cotyledonary node cells by
The successful application of biotechnology in crop improvement is based on efficient plant regeneration protocol. Soybean has been considered as recalcitrant to regenerate
The nutritional requirement for optimal shoot bud induction from different explants has been reported to vary with mode of regeneration. Media compositions have a key role in shoot morphogenesis, the basal medium MS  is most commonly used for soybean organogenesis and the medium B5  are useful in some approaches. Benzylaminopurine (BA) has been the most commonly used plant growth regulator either alone or in combination with a low concentration of cytokinins, kinetin or thidiazuron (TDZ) [22, 39]. TDZ was reported to induce multiple bud tissue (MBT) from cotyledonary node axillary meristem which then gives shoots in the presence of BA . The efficiency of shoot bud formation were enhanced by supplementing media with proline, increased level of MS micro nutrients , and ureide in the form of allantoin and amides .
Adventitious shoot regeneration from cotyledonary node or leaf node is based on proliferation of meristems. Use of pre-existing shoot meristems in transformation procedures can increase the chance of chimerism, so identifying tissues that can produce shoots in the absence of such pre-formed organs would be important . Adventitious soybean shoots have been induced from hypocotyls ; cotyledons [18, 20], primary leaves  and epicotyls . Hypocotyls of seedlings have been used as explants for adventitious shoot regeneration by Kaneda et al. . Explants cultured on media supplemented with TDZ induced adventitious shoots more efficiently than BA. Histological analysis of adventitious shoot regeneration from the hypocotyl shows shoot primordias, formed from parenchymatous tissues of central pith and plumular trace regions . Hypocotyls of seedlings have seldom been used as explants, even though the shoot regeneration frequency from hypocotyl segments was found to be higher than from cotyledons . Franklin et al.  investigated the factors affecting adventitious shoot regeneration from the proximal end of mature and immature cotyledons. The presence of BAP and TDZ in the medium exerted a synergistic effect, in that regeneration efficiency was higher than for either cytokinin alone.
Indirect organogenesis is important as an alternative source of genetic variation in order to recover somaclones with interesting agronomic traits. Callus regeneration is advantageous over direct regeneration for transformation since effective selection of transgenic cells can be achieved . However, the efforts made to regenerate plants from callus have yielded poor results since plants could not be regenerated from any type of soybean callus . Yang et al.  compared different explants excised from immature and germinated seeds for callus mediated organogenic regeneration, although induction of organogenic callus was easily achieved by culture of immature cotyledons, development of adventitious buds from these calluses and the subsequent growth of these buds to shoots were inefficient, suggesting that only part of the callus was competent for regeneration. Sairam et al.  developed a rapid and efficient protocol for regeneration of genotype-independent cotyledonary nodal callus for cultivars Williams 82, Loda and Newton through manipulation of plant growth regulators and carbohydrates in the medium. Hong et al.  reported organogenic callus induction from cotyledonary node and leaf node explants in media supplemented with TDZ and BA, the system has been successfully utilized for
Among the different factors affecting soybean regeneration, the genotypic dependence is ranked quite high. Since there is strong genotype specificity for regeneration of different soybean genotypes, a major limiting factor, it is pivotal to formulate genotype specific regeneration protocols. Genotype specificity for regeneration in soybean is well documented, although organogenesis is less genotype dependent and has become routine in several laboratories [18,20,28,29&33]. Reichert et al.  tested organogenic adventitious regeneration from hypocotyl explants excised from 18 genotypes. Plant formation from hypocotyl explants showed that all genotypes were capable of producing elongated shoots that could be successfully rooted. This study confirmed the genotype independent nature of this organogenic regeneration from the hypocotyl explant. Sairam et al.  developed an efficient genotype independent cotyledonary nodal callus mediated regeneration protocol for soybean cultivars Williams 82, Loda and Newton developed through manipulation of plant growth regulators and carbon source. Callus induction and subsequent shoot bud differentiation were achieved from the proximal end of cotyledonary explants on modified MS  media containing 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA), respectively. Sorbitol was found to be the best for callus induction and maltose for plant regeneration. The genotypic dependence of regeneration from cotyledon explants could be reduced by the use of combinations of cytokinins (Franklin et al. ). Though there was no significant difference in shoot bud formation among different genotypes, but there was significant difference in conversion of the number of regenerated plants in each cultivar (Delzer et al. ).
Agrobacterium mediated transformation
2.4. Particle bombardment
Even though particle bombardment is a widely used technique for transforming soybean embryogenic cultures, it was rarely explored for shoot morphogenesis. McCabe et al.  was the first to report particle bombardment mediated transformation in soybean. Transforming meristems of soybean bu DNA coated gold particles followed by shoot regeneration in the presence of cytokinin, resulting in the development of chimeras. In subsequent studies, non-chimeric plants were obtained through the use of screening methods for the selection of plants that contained transgenic germ-line cells [32,62&63]. Shoot apex transformation is labour intensive because the meristematic tissue is diffcult to target and, without selection, a large number of plants must be regenerated and analysed .
2.5. Genes for trait improvement
Soybean has been improved by
3. Somatic embryogenesis and transformation
Somatic embryogenesis is a process by which a plant somatic cell develops into a whole plant without gametic fusion but undergoes developmental changes as that of zygotic embryogenesis [71, 72]. The first demonstration of
3.1. Somatic embryogenesis
The first record of soybean somatic embryogenesis was reported by Beversdorf & Bingham , followed by Christianson et al.  who regenerated plants through the method. The immature cotyledon is the preferred explant for soybean somatic embryogenesis as it has pre-determined embryogenic cells. Somatic embryogenesis is a multi-step regeneration process starting with the formation of proembryogenic cell mass, followed by somatic embryo induction, their maturation, desiccation and finally plant regeneration .
Soybean somatic embryos were induced from immature cotyledon explants cultured on medium containing high levels of 2,4-D . Even though NAA induced somatic embryogenesis from immature cotyledons, the mean number of embryos produced on 2,4-D was significantly higher . Explant orientation, pH, solidifying agent, and 2,4-D concentration have a synergic effect on somatic embryo induction . The early-staged somatic embryos can be maintained and proliferated by subculturing the tissue on either semi-solid medium  or liquid suspension culture medium . Somatic embryos incubated in a medium containing NAA do not proliferate so well as those produced on a medium containing 2,4-D . Somatic embryos initiated on NAA are more advanced in embryo morphology than those induced on 2,4-D and the efficiency of somatic embryo induction was highest with a medium containing 2-3% sucrose. Cultures initiated on lower sucrose concentrations tended to produce a higher amount of friable embryos, while increased concentrations of this sugar impaired embryo induction [80,84-86]. Histodifferentiation and maturation of somatic embryos doesn’t need exogenous auxin or cytokinins . Indeed, poorly developed meristem or swollen hypocotyls may be an undesired outcome of the application of exogenous auxins and cytokinins, respectively. Moon and Hildebrand,  investigated the effects of proliferation, maturation, and desiccation methods on conversion of soybean somatic embryos to plants. Somatic embryos proliferated on solid medium showed a higher regeneration rate when compared with the embryos proliferated in liquid medium. The growth period of somatic embryo development can be reduced one month by culturing in a medium devoid of 2,4-D and B5 vitamins. Carbon source is critical for embryo nutritional health and improves somatic embryo maturation. The effects of carbohydrates on embryo histodifferentiation and maturation on liquid medium were analyzed by Samoylov et al. . FNL medium supplemented with 3% sucrose (FNL0S3) or 3% maltose (FNL0M3) were compared. Data indicated that sucrose promotes embryo growth and significantly increases the number of cotyledon-stage embryos recovered during histodifferentiation and maturation. However, the percentages of plants recovered from embryos differentiated and matured in FNL0S3 was lower than those grown in FNL0M3 (Samoylov et al. 1998b). The quality of somatic embryos can be positively influenced by a low osmotic potential in maturation medium [90, 91]. Carbohydrates can act as an osmotic agent. Polyethylene glycol 4000, mannitol and sorbitol were tested as supplements to a liquid Finer and Nagasawa medium-based histodifferentiation/maturation medium FNL0S3, for soybean (
Soybean somatic embryogenesis is highly genotypic when compared to organogenesis. The existence of strong genotype specificity in the regeneration capacity of the different cultivars represents a major limiting factor for the advancement of soybean biotechnology. The embryogenic efficiency of soybean was shown to be different among cultivars at each stage (induction, proliferation, maturation, germination) of somatic embryogenesis [92,96] and it is very challenging to identify genotypes highly responsive to all stages. Simmonds and Donaldson,  screened 18 short season soybean genotypes for proliferative embryogenesis. Five genotypes produced embryogenic cultures which were proliferative for at least 6 months. Yang et al.  screened 98 Chinese soybean varieties for somatic embryogenesis and selected 12 varieties based on their embryogenic capacity. The greatest average number of plantlets regenerated per explant (1.35) was observed in N25281. Bonacin et al.  demonstrated the influence of genotype on somatic embryogenic capability of five Brazilian cultivars. Droste et al.  reported somatic embryo induction, proliferation and transformation of commercially grown Brazilian soybean cultivars for the first time. Soybean somatic embryo conversion is genotype dependent; germination frequency of H7190 was approximately three fold lower than that of PI 417138 . Hiraga et al.  examined the capacity for plant regeneration through somatic embryogenesis in Japanese soybean cultivars and identified Yuuzuru and Yumeyutaka as having high potential for somatic embryogenesis. Several cultivars were identified as uniformly embryogenic at the primary induction phase at all locations, among which Jack was the best . Kita et al.  evaluated somatic embryogenesis, proliferation of embryogenic tissue, and regeneration of plantlets in backcrossed breeding lines derived from cultivar Jack and a breeding line, QF2. The backcrossed breeding lines exhibited an increased capacity for induction and proliferation of somatic embryos and were used successfully to generate transgenic plants.
Agrobacterium mediated transformation
Recovery of the first transgenic plant
3.4. Particle bombardment
Particle bombardment is a widely used technique for transformation of embryogenic cultures of soybean; the major advantage of this technique over
3.5. Genes for trait improvement
Li et al.  attempted to transform two antifungal protein genes (
4. Conclusion and future prospects
As demands increase for soybean oil and protein, the improvement of soybean quality and production through genetic transformation and functional genomics becomes an important issue throughout the world. Modern genetic analysis and improvement of soybean heavily depend on an efficient regeneration and transformation process, especially commercially important genotypes. The transformation techniques developed until now till date do not allow high-throughput analyses in soybean functional genomics; though significant improvements have been made in the particle bombardment of embryogenic culture and
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