Differences between bacterial and eukaryotic N-linked glycosylation.
N-linked glycosylation is of paramount importance for the biopharmaceutical sector given that approximately 40% of all approved therapeutic proteins and eight of the top ten selling biologics of 2010 contain N-linked oligosaccharides [1, 2]. With specific cell productivities in biotechnological processes estimated to reach their theoretical maximum in the near future, and despite anticipated further increases in culture density, the scope for improvement of biotherapeutics is limited in terms of production volume . As a result there is an increased focus on biotherapeutic efficacy in the development and production stages. Modification of the glycoform is a target for drug design as it can enhance efficacy, mode of action and half-life significantly . Simultaneously, N-linked glycosylation plays a key role in the safety of biotherapeutics. Certain N-linked oligosaccharides bound to therapeutic proteins have been found to trigger undesired effects in patients [5-7] thus deeming them a safety concern during process development .These elements make N-linked glycosylation a key target for quality control both from the therapeutic efficacy and safety standpoints. A well-defined product may have consistent protein backbones but still a glycoform distribution of more than a hundred detectable isoforms . Narrowing and targeting the glycan profile is expected to improve efficacy and safety significantly. An example of the variety of reported glycoforms found on the crystallisable fragment (Fc) of monoclonal antibodies (mAbs) is presented in panel D of Figure 1. It is worth noting that single monosaccharides (e.g. core Fucose) on the complex glycans present on the crystallisable fragment (Fc) of monoclonal antibodies (mAbs) may alter the therapeutic efficacy of these products.
1.1. Effect of glycomic profile on therapeutic efficacy
In 2001, when the human genome was fully sequenced, it was found that it contains fewer genes than expected. However, this might be compensated by the diversity found at the level of protein architecture, structure, transcription patterns, and posttranslational modifications of which glycosylation is the most complex . Glycan moieties have a very diverse number of roles in biological systems, making them relevant for biotherapeutics. Starting with the co-translational attachment of glycan structures, it was suggested by Petrescu et al. that glycan structures act as nucleation sites for remote aromatic parts in an amino acid sequence in order to begin the folding process . Although glycans play an important role in local as well as global folding of proteins it has to be pointed out that they are often not essential to maintain structure [16-18], as is the case for Interferon-β (IFN-β), for example. This naturally occurring glycoprotein is produced in the human body in response to viral infections or other biological threats . Human IFN-β contains a conserved Asn-80 glycan attachment site and as a biotherapeutic, is marketed for the treatment of multiple sclerosis.
There are currently four IFN-β products marketed under the names of AVONEX®, Rebif®, Betaseron® and Extavia®. Crucially, the licensed drugs AVONEX and Rebif® are produced in Chinese hamster ovary (CHO) cells and are therefore fully glycosylated products while Betaseron® and Extavia® are produced in Escherichia coli resulting in an aglycosylated pharmaceutical . AVONEX® and Betaseron® were compared side by side and it was found that the glycosylated form had a 10-fold increased anti-viral assay activity as compared to the
1.1.2. Monoclonal antibodies
Monoclonal antibodies are currently the highest selling biotechnology products and by 2016 are predicted to make up five of the top ten selling pharmaceutical products worldwide . Currently, there are over 30 FDA approved mAbs which are prescribed for the treatment of cancer and auto-immune disorders . With a total of 205 reported antibodies under trial , the total number of approved antibody therapeutics is expected to increase significantly in the foreseeable future. The concept of a “magic bullet” that would go directly to the intended target epitope, yet remain harmless in healthy tissue was introduced in the late 19th century by Paul Ehrlich . Key elements for realising Ehrlich’s vision were the development of hybridoma cells by Köhler and Milstein to produce antibodies of predefined specificity  and the use of phage display libraries for antibody humanization by Greg Winter and Richard Lerner [28, 29]. Antibodies are generally described as symmetric structures which, in humans, are composed by a pair of heavy chains and an additional pair of light-chains covalently bound together by disulphide bridges. There are five heavy chain isotypes (IgA, IgG, IgE, IgG and IgM) and two light chain isotypes. The spatial arrangement of the four peptide chains results in the Y-shaped structure depicted in 2.
Both the heavy and light chains contain hypervariable segments also referred to as complementary determining regions (CDR). While the CDR is the antigen binding site, located in the antigen binding (Fab) region and responsible for antigen binding, the constant Fc region is responsible for effector functions such as activating the complement cascade or binding to Fc receptors. 80% of all antibodies found in the blood plasma are of the IgG isotype (with a molecular weight of approximately 150kDa) and until today only IgG-type mAb products have been licensed as biopharmaceuticals . As a consequence only the IgG isotype of antibodies and their biological mechanisms will be discussed. Two fundamental modes of biological action of IgG class antibodies are discussed below:
1.1.3. Fab region glycans
It is well established that the glycan moieties in the Fc region of therapeutic antibodies have considerable effects on in vivo mechanism and pharmacokinetics. However, the Fab region can also be used to enhance characteristics of therapeutic antibodies. It has been estimated that between 20-30% of antibodies also carry a glycan structure in the Fab region, where a potential glycosylation site can be found on both the heavy as well as the light chain [43, 44]. Although the role of variable region carbohydrates is not clear, it is suggested that glycan moieties may influence antigen affinity, specificity, antibody solubility and stability hence, limiting aggregation [45, 46]. A further biological role of Fab region glycans is believed to be
A further glycoprotein of biopharmaceutical importance is Erythropoietin (EPO), a hormone that binds to the receptors of red blood cell precursors in the bone marrow leading to their survival, proliferation and differentiation, thus increasing the red blood cell count [49-51]. EPO is used in the treatment of anaemia associated with a number of disease states such as chronic renal failure, cancer and HIV infection [52-55]. EPO has a total of four glycan attachment sites, three N-linked sites at amino acid positions 24, 38 and 83,and one O-linked site at amino acid position 126 [56, 57]. The four glycan moieties have been estimated to contribute approximately 40% to the total molecular mass of EPO and probably cover much of the surface of the molecule . The hypothesis of full surface exposure is further supported by an analysis of the glycan moieties, which revealed predominantly fucosylated and tetraantennary complex glycans at the N-linked glycosylation sites  and, as such, suggest surface exposure during enzymatic processing. The glycan structures have been shown to play an important role in biological activity. For example, it is known that higher glycan antennarity leads to an increase in EPO’s
1.2. Desired characteristics dependent on application
Erythropoietin is also a very good example to demonstrate how desired safety and efficacy characteristics can be enhanced through glycan engineering of existing biotherapeutics. The plasma clearance of EPO is regulated by sialic acid containing carbohydrates [58, 63], where an increase in sialic acid moieties is known to increase glycoprotein half-life and would also explain why higher antennarity is linked to increased plasma half-life. An investigation into EPO glycan structures and biological activity showed that desialylated structures showed increased
2. Current methodology and future application of quality by design initiative
Drug development begins with the discovery of molecules that have shown the biochemical potential to treat illnesses. Based on manufacturability and potential profitability, drug candidates are then selected for optimization and, eventually, for preclinical and clinical trials. To ensure that sufficient material is available for the different phases, manufacturing is concurrently scaled up during this stage in process development. Also throughout these stages, all the data regarding drug safety, efficacy and manufacture is reviewed for approval by the corresponding regulatory agencies. Approval requires that the drug product is produced consistently and that it is safe and efficacious for its indication. Despite decades of advances in drug product manufacturing, pharmaceutical process development and approval is still extremely lengthy, highly expensive and uncertain .
In order to reduce their losses, manufacturers have traditionally relied on the so-called quality by testing (QbT) approach for drug development and approval . In QbT, product quality attributes (the ranges for drug substance properties that yield acceptable safety and efficacy) are linked with a specific manufacturing process and its corresponding set of inputs (raw materials and process parameters) during clinical phases of development. The process inputs that empirically show to yield acceptable product quality are defined and are often maintained unchanged after phase II clinical trials so that manufacturers avoid costs associated with additional testing for regulatory compliance . During manufacturing, the process inputs are controlled to remain at their pre-defined set points, and at the end of each batch, the product is tested for compliance with the desired quality [68-71]. This black box approach does not require mechanistic knowledge that relates process inputs with product quality, and because of this, the QbT approach uncouples product end quality from the manufacturing process. The main drawbacks of QbT are [10, 69, 72]:
Development and approval of pharmaceutical processes under QbT has proven to be extremely time-consuming (between 7 and 10 years [73, 74]) and very expensive (US$1.2 to 1.8 billion per approved drug when the risk of failure is included [67, 75]). This could be attributed, in part, to limited understanding of the relationship between product quality characteristics, therapeutic mechanisms and the effect process inputs have on quality.
Process control is not established by mechanistic links between inputs and desired product quality. Due to this, the process is susceptible to generate off-spec product, and when this occurs, identifying the source(s) of failure is difficult.
The range of process conditions approved under QbT is narrow and changes to process conditions outside this range require additional approval, which eventually translates into further delays and expense. This discourages pharmaceutical companies from modifying or optimizing current processes and implementing innovative ones.
Overall, processes developed and approved under QbT generate a limited amount of knowledge because mechanistic relations between inputs and outputs are not apparent. This greatly restricts transferability of the knowledge gained from one process/product to the next. Moreover, the lack of generated mechanistic information increases the likelihood suboptimal process performance.
Therapeutic proteins currently undergo the same QbT development and approval process as their small molecule counterparts and suffer the same caveats. In the QbT context, bioprocess inputs are empirically defined so that the quality properties (e.g. aggregation, folding, methylation and glycosylation) of the protein lie within ranges that yield acceptable safety and therapeutic efficacy. As occurs with small molecules, QbT disjoins therapeutic protein quality from the bioprocess. This has led to inadequate understanding of the relationship between process inputs and product quality and has greatly limited the potential for bioprocess optimization.
The inefficiencies associated with the QbT approach along with more stringent regulatory requirements have led to manufacturers investing more in drug discovery than process understanding and optimization, which in turn, has translated into a decrease in the cost-effectiveness, number and quality of innovative drugs and pharmaceutical manufacturing processes [67, 69]. In order to overcome these limitations, regulators and industry specialists have proposed the implementation of Quality by Design (QbD) concepts to the manufacture of all new drugs, including therapeutic proteins, in the development pipeline [68, 72, 76].
2.1. Quality by design initiative in pharmaceuticals and how it is expected to affect biopharmaceuticals in the future
Pharmaceutical QbD is a conceptual framework for the development and approval of pharmaceutical manufacturing processes that aims to build quality (particularly with respect to safety and therapeutic efficacy) into the product at every stage of process development [10, 71, 72]. Application of QbD principles to pharmaceutical process development is outlined in the Process Analytical Technology (PAT) guideline “PAT - A Framework for Innovative Pharmaceutical Manufacturing and Quality Assurance”  by the US Federal Drug Administration (USFDA) and in the guidance documents “ICH Q8 Pharmaceutical Development” , “ICH Q9 Quality Risk Management”  and “ICH Q10 Pharmaceutical Quality Systems”  from the International Conference on Harmonization (ICH), which is an association constituted by the USFDA, the European Medicines Agency (EMA), the Pharmaceuticals and Medical Devices Agency of Japan (PMDA) and several experts from the pharmaceutical industry.
Implementation of QbD to pharmaceutical manufacturing processes is an information-driven process where all available knowledge on the drug product including, but not limited to, its therapeutic mechanisms, its process of manufacture and potential sources of variability is used to define a range of manufacturing conditions that will ultimately ensure product safety and efficacy when administered to patients. More specifically, the ICH guidelines [12, 71, 78] define the following requirements for the implementation of QbD to pharmaceutical processes:
2.1.1. Definition of the quality target product profile (QTPP)
The QTPP is defined as the set of quality characteristics that would ideally be achieved to ensure that the drug product is safe and efficacious. Considerations to define the QTPP include the route of administration, dosage form, delivery systems, dosage strength, sterility, purity and stability .
2.1.2. Identification of the critical quality attributes (CQAs) of the drug product
A CQA is defined in ICH Q8 as “a physical, chemical, biological, or microbiological property or characteristic that should be within an appropriate limit, range, or distribution to ensure the desired product quality” . As the definition implies, identification of CQAs requires thorough physicochemical and biological characterization of the drug product and in-depth knowledge on which of its properties have a higher influence on its safety and efficacy.
2.1.3. Identification of process inputs that affect product CQAs
Once CQAs are identified, it is necessary to determine not only which process inputs (raw materials and process conditions) impact CQAs, but also how these inputs interact to affect the drug product’s CQAs. We must note that much of the required knowledge may not be available for certain processes (particularly novel ones) and should be established through the combination of prior knowledge, mechanistic modelling, experimentation and finally, a risk assessment so that the influence of material attributes and process conditions on CQAs is ranked according to likelihood and extent of impact. It is worth mentioning that, under QbD, there is special emphasis on design of experiments (DoE) so that the interaction between individual process inputs and their impact on product CQAs is represented. Crucially, all elements involved in this section directly couple manufacturing process conditions with the CQAs of the drug product in a robust, systematic and information-driven manner.
2.1.4. Selection of the appropriate manufacturing process
With the ranking obtained through the risk assessment mentioned above, a multi-dimensional design space of allowable process input values (and combination thereof) is defined. ICH Q8 defines the design space as “an established multidimensional combination and interaction of material attributes and/or process parameters demonstrated to provide assurance of quality” . Using the design space as a guide, the manufacturing process which is most capable of maintaining process conditions within the ranges that ensure product quality is defined. The selected manufacturing process must be robust such that it minimizes the risk of process conditions falling outside the design space, thus increasing the likelihood of the CQAs being within the range that ensures safety and efficacy.
2.1.5. Definition of a control strategy
With the appropriate manufacturing process in place, a strategy to mitigate the risk of materials and process conditions falling outside acceptable ranges must be established. By applying this risk management strategy, raw material specifications are monitored and controlled such that no impact is observed on the drug product’s CQAs. In addition, the process parameters that influence the CQAs are controlled (ideally through online measurements and tight control systems) at every stage of the manufacturing process so that the desired product quality is met.
Conceptually underlying all the elements that constitute QbD implementation is process analytical technology (PAT). PAT is defined by the USFDA and the ICH as “a system for designing, analyzing and controlling manufacturing through timely measurements of CQAs and performance attributes of raw and in-process materials and processes with the goal of ensuring final product quality” . The elements of PAT concerning process design and control have been presented in the description of QbD above. However, more discussion must be provided on the “timely measurement of CQAs and performance attributes”  throughout the implementation of QbD to manufacturing process development. It is clear that, from very early stages of QbD-driven process development, methods for accurately identifying and measuring the physical, chemical and biological properties of drug products are essential for defining QTPPs and CQAs. Further along in the implementation of QbD, it is also necessary to accurately measure the material attributes and process parameters that affect product CQAs. Finally, and as the definition of PAT states, once the manufacturing process is selected, appropriate analytical technologies are necessary to monitor it is necessary to measure process parameters in a timely fashion (ideally online) so that the generated data can be used for process control. From this, it is clear that analytical methods constitute a core element throughout process development under the QbD scope .
Adoption of the QbD in pharmaceutical process development aims to address all of the limitations described above. The three major regulatory bodies (USFDA, EMA and PMDA) are encouraging implementation of the QbD approach for the development of all new drugs in the pipeline [68, 70, 72, 80]. QbD is expected to reduce process approval time and costs, reduce regulatory intervention and encourage optimization and innovation by building processes around the mechanistic relationships between inputs and product quality. Because these relationships should be based on sound science and engineering principles, process outputs are more predictable and require less regulation which, in turn, would considerably reduce approval time and development costs. In addition, the more ample design space created through QbD would allow inputs to vary more without the need for additional approval. Predictability would also translate into much tighter control systems that would dramatically reduce the likelihood of generating product with unacceptable quality. Finally, the wealth of knowledge generated by the QbD approach along with the broader design space and more flexible regulatory approval characteristics would encourage process optimization and could potentially contribute to the development of novel processes as well as the discovery and design of next generation drugs.
Since the guidelines for QbD were first drafted, the framework has been implemented in the field of small-molecule therapeutics (SMTs) with relative ease. In contrast, the implementation of QbD to protein therapeutics (PTs) has met more resistance. This is likely due to the fact that the physical and chemical processes underlying the manufacture of SMTs is better understood and the mechanisms relating process inputs with SMT quality are easier to define. Conversely, the mechanisms by which living organisms produce PTs are less well understood, and the structural complexity of PTs makes their isolation, separation, purification and overall quality control much more challenging. Despite this, the regulatory agencies and several authors believe that sufficient knowledge is available or can be gained through current experimental and modelling methodologies to elucidate mechanistic relations between bioprocesses and PT quality, thus allowing for implementation of QbD principles to the development of therapeutic protein manufacturing processes in the near future [68, 72].
Implementation of the QbD paradigm in biopharma should increase knowledge on the therapeutic mechanisms of biotherapeutics considerably. This could lead to the improvement of previously existing products and may contribute to the discovery and development of new biologics. It will also generate a rich and systematized knowledge base relating manufacturing conditions with drug products which may lead to more robust process control, process optimization and, potentially, development of novel and efficient platforms for the manufacture of biological therapeutics. Implied in this is the considerable reduction in approval times which would heavily reduce costs of product and process development and eventually translate into lower costs for healthcare providers and patients.
2.2. Other critical quality attributes of protein-based therapeutics
Several drug characteristics other than glycosylation are considered critical quality attributes due to their impact on biological activity, pharmacokinetics or pharmacodynamics, and safety in terms of immunogenicity and toxicity. These can arise from variations in protein structure or from the presence of other adventitious molecules in the product formulation. Protein aggregation is a common protein-related CQA, which can occur at any stage of protein production or processing due to the protein structure, which may leave hydrophilic patches exposed, its concentration in the preparation or the process conditions. Temperature and pH extremes or physical stress can increase a protein’s propensity to aggregate. Although protein aggregation is known to affect efficacy, the main concern is the immunogenicity of aggregates [81, 82]. Protein aggregation may also be the result of modification reactions, such as oxidation, which is caused by increased levels of reactive oxygen species. This can additionally affect function, as, for example, in the case of antibodies where oxidation can alter the Fc structure and thus reduce its binding affinity . Conformational changes can also occur at refolding steps of bacterial production platforms and are potentially immunogenic.
Other possible structural changes include protein fragmentation, due to proteolytic enzymes present in the cell culture supernatant or in human plasma, extreme pH or temperature conditions, or because of chemical disruption of peptide bonds, C- and N-terminal truncation and deamidation. The effect of fragmentation is product-dependent, however it is known that it can impact biological activity, serum half life and immunogenicity due to the generation of novel epitopes [84, 85], whereas the remaining aforementioned changes do not appear to adversely affect product potency or safety [86-89], with the exception of deamidation within a complimentarity-determining region, which can affect biological activity . Finally, glycation is another post-translational modification that involves the chemical addition of a monosaccharide on the side chain of a lysine residue. It occurs when a protein is incubated in the presence of reducing sugars, especially fructose and galactose and to a lesser extent glucose, in cell culture , and can affect its biological activity .
In addition to the product-related critical quality attributes described above, the host cell line, raw materials and process operation can introduce impurities or contaminants with adverse effects on the formulation’s suitability for
2.3. Challenges in implementing QbD in biopharma
Implementation of QbD to bioprocesses first requires thorough characterization of the drug substance (i.e. the therapeutic protein) in order to determine the attributes that define its safety and efficacy. Drug substance characterization, which in many cases is a challenging task, is usually done with liquid chromatography peptide mapping combined with mass spectrometry for amino acid sequencing [97-99], x-ray crystallography for three dimensional structure and different analytical chromatographic or electrophoretic techniques coupled to mass spectrometry for the analysis of N and O glycans which have been extensively reviewed by del Val et al . The propensity of protein aggregation and fragmentation is usually measured through size exclusion chromatography [100-102]. By coupling these techniques to non-clinical (in vitro assays), preclinical and clinical trials, the CQAs for protein therapeutics are defined. However, despite the available methods for characterizing proteins and their CQAs, these are not always deemed sufficient to ensure identity. This is evidenced by current US and European legislation concerning Biosimilar products. Both the USFDA and the EMA have required phase I and phase III clinical trials in order to establish that a follow-on product is similar to its brand-named counterpart even when they have been shown to have equivalent CQA attributes according to the available analytical methods . Lack of confidence in the current analytical techniques for drug substance characterization is a critical challenge that must be overcome for appropriate implementation of QbD to biopharmaceutical processes. Development of additional characterization methods are required to compare the product CQAs with the QTPPs defined during the early stages of process development. Furthermore, development of additional non-clinical studies for determining product safety and efficacy is required.
The next step under the QbD scope is to define the manufacturing process, the process inputs that affect product CQAs and the mechanisms by which this occurs. In contrast to their small-molecule counterparts which are largely produced
After defining the design space, a control strategy must be defined so that process inputs are maintained within the range that ensures product quality. A crucial challenge in defining the control strategy is the ability to measure the process inputs that influence product CQAs. The QbD and PAT guidance documents suggest that control should be established through timely measurements which, ideally, should be performed online. This, again, is not trivial in a biological system. The interior of a bioreactor is a complex environment mainly composed of culture medium, cells, product and co-products of cell culture. The culture medium itself is a complex mixture of nutrients. Many of the common process parameters are readily measurable such as pO2, pCO2, temperature cell density and certain metabolite concentrations. However, many of the single components that influence product CQAs are difficult to measure in such a complex mixture. Several analytical methods that have the ability of tracking single components in bioreactors are currently being explored. Some of the most promising technologies are based on infrared spectroscopy, and have been reviewed recently by Landgrebe et al. . Successful implementation of these techniques may lead to absolute online monitoring and process control. In parallel, methods to measure the intracellular concentration of key nutrients and metabolites online are being developed with promising results . On the other hand, mathematical modelling efforts are being developed to describe complex biological processes such as N-linked glycosylation. This work has yielded encouraging results for bioprocess control and optimization, and could potentially aid in cell line development for third generation therapeutic proteins.
Almost by definition, QbD is a self-catalytic process because it relies on, and generates, a wealth of information. The more QbD is implemented in bioprocess development and approval, further understanding will be gained and fed into the development of newer processes which will eventually culminate in near-complete description of therapeutic mechanisms, drug product CQAs and therapeutic protein bioprocessing.
3. Overview of production organisms and manufacturing environment
The majority of approved biopharmaceuticals are produced in mammalian cell culture systems, since they are the sole means to deliver proteins with desired glycosylation patterns and thus ensure reduced immunogenicity and higher
As in April 2012, there are 77 therapeutic glycoproteins out of the total 642 drugs approved by the European Medicines Agency (EMA). Host systems for their production include mammalian cells (65 drugs) and transgenic animals (2 drugs), while several are isolated from the blood plasma of healthy donors (10 drugs), as depicted in Figure 4A. Therapeutic classes of each glycoprotein drug are also presented in Figure 4B and involve mainly: hereditary diseases (Haemophilia A and B, Fabry disease, Gaucher disease, and others; 29% of EMA approved glycoproteins), cancer (leukemia; cancer in ascites; thyroid, stomach, breast, colorectal, etc. cancers or anaemia caused by chronic cancer; 26% of EMA approved glycoproteins), and autoimmune disorders (rheumatoid arthritis, multiple sclerosis, Crohn’s disease, Lupus Erythematosus; 18%). Other therapeutic areas for which glycoprotein drugs are prescribed include infertility, acquired injuries/disease (tibial fractures, spondylolisthesis, myocardial infarction), immunosuppresants during transplantations, for hemostasis after surgery, for anaemia caused by chronic kidney disorders, postmenopausal diseases (osteoporosis) and one against the respiratory syncytial virus (containing a monoclonal antibody as an active substance: Synagis®).
The drug categories of current EMA approved glycoproteins (Figure 4C), based also on the classification in  include in descending order of approved drugs: polyclonal (mostly human plasma derived drugs) and monoclonal antibodies (mAbs), growth factors (e.g. erythropoietin, stimulating factors, bone morphogenetic proteins), blood factors and coagulants (e.g. factors: VIII, VIIa, IX and fibrinogen & thrombin), therapeutic enzymes (e.g. recombinant β-glucocerebrosidase), hormones (e.g. follitropin, lutropin), fusion proteins (e.g. Enbrel® the extracellular ligand-binding portion of human tumor necrosis factor receptor p75 linked to an analog human Fc portion of human IgG1), and serpins (serine protease inhibitors, e.g. C1 esterase inhibitor). Other classes involve thrombolytics and anticoagulants (e.g. tissue plasminogen activator and protein c, respectively) and cytokines (e.g. interleukins and interferons).
Two other glycoproteins (Leukoscan® and Scintinum®) have been approved by EMA,but are used for radionuclide imaging rather than therapeutics and thus have not been included in the statistics shown in Figure 4A. Furthermore, denosumab a monoclonal antibody produced by Amgen is approved under two brand names Prolia® (for postmenopausal osteoporosis) and as Xgeva® (for bone metastasis in cancer), and hence only one has been included in final list of approved drugs. Two more mAbs have been approved for use in specific EU countries but are not approved by EMA and hence have also not been included (Orthoclone OKT3® and Reopro®). Finally, two drugs that contain the recombinant factor VIII as active ingredient are produced by Bayer Pharma AG using baby hamster kidney (BHK) cells as host cells from an identical fermentation procedure, but since they are purified through slightly different downstream processes, both have been included separately in the statistics shown in Figure 4. Vaccines are also not included in the list of glycoproteins because their production mainly involves the propagation of viruses and not specific glycoprotein production. Moreover, all recombinant vaccines are produced in microbial organisms and hence do not involve glycoprotein production, but amino acid sequences of antigens that are not glycosylated.
CHO (Chinese hamster ovary) cells are the dominant host cells for the production of glycoproteins as far as the approvals in European Union are concerned, with 47 out of the total 77 therapeutic EMA approved glycoprotein drugs using them as host cells. Five drugs produced in CHO cells are biosimilars of recombinant erythropoietin (with reference product being a recombinant epoetin produced from Janssen-Cilag GmbH with brand names Eprex® in UK and Erypo® in Germany). All aforementioned biosimilars were approved in 2007. Biosimilars are defined from the EMA as drugs similar to a biological drug already in the market that are used to treat the same disease and within the same range of doses. Hybridoma cells are the second most frequently used hosts for recombinant glycoprotein production (12 drugs), followed by glycoproteins purified from blood plasma from healthy donors (10 drugs). BHK cells, along with HT-1080 cells, a human sarcoma cell line, with three approved drugs from each cell line are fourth. All approved drugs produced in BHK cells involve blood factors. Regarding HT-1080 cell line, all of the drugs produced from it belong to Shire pharmaceuticals portfolio. The active substances of these drugs involve therapeutic enzymes, while these drugs are also classified as orphans. Orphan drugs, according to the EMA, are prescribed to treat diseases that do not affect more than 5 in 10,000 individuals in the European Union at the time that the drug was submitted for approval. Finally, the host system classification list is completed with transgenic animals. Currently, two drugs are produced from transgenic animals and involve two serpins (serine protease inhibitors): antithrombin III (Atryn®) and C1 esterase inhibitor (conestat alfa; Ruconest®) isolated from goat and rabbit milk, respectively.
3.1. Mammalian cell systems
Mouse myeloma cell lines SP2/0 and NS0 are frequently used for research purposes , while human embryonic kidney 293 (HEK293) cells are often utilized to produce material for pre-clinical trials or research . CHO cells are the workhorse of industrial production because of their ability to do gene amplification, which increases the level of product specificity and the ability to grow in serum-free suspension conditions . Similarly, the myeloma cell-derived rodent NS0 cells have a high efficiency in producing recombinant immunoglobulin proteins and they can be cultured in both serum or serum/protein free suspension which reduces manufacturing costs during large-scale protein production . Since CHO and NS0 originate from different mammalian species, the amount and type of glycosidases and transferases they have varies. Studies have reported that NS0 cells can produce glycoproteins that are highly immunogenic to human .
Human cells, on the other hand, are alternative hosts. The fact that human cells generate recombinant products with PTMs similar to the native counterparts secreted inside the human body gives them an advantage over CHO and NS0, which lack some essential glycosylation enzymes, e.g. bisecting N-acetylglucosamine transferase and 2-6 sialyltransferase. HEK 293 cells is the most-widely used human cell line for research into recombinant protein production and studies show that HEK 293 cells are capable of manufacturing Xigris (activated protein C) with proper -carboxylation and propeptide digestion at its glutamate amino acid residues, which CHO cells fail to offer . In addition, the main advantage of protein expression in human cells is the low level of immunogenic reactions, with limited expression of N-glycolylneuraminic acid (Neu5Gc)-bearing erythropoietin (EPO) in human HT1080 fibrosarcoma cell line than the CHO-derived ones . Some new human-based expression cell lines have been developed and are increasingly adopted by industry. For example, the Per.C6 cell line derived from the human retina cells have shown to give a protein titre of more than 2 g/L in simple fed-batch culture . Unlike CHO cells, which often require product selection, the Per.C6 cell line does not require any gene amplification or selection strategy, nor does it need a large gene copy number for stable protein expression . Per.C6 derived-EPO is free of Neu5Gc thus limiting immunogenicity .
3.2. Microbial cell systems
Microbial cell systems from both eukaryotic and prokaryotic origin are commonly used for the expression of heterologous proteins. They have long been established for use in the production of recombinant proteins. This is due to their ease of use, the vast wealth of genetic and biochemical knowledge which has amassed, high protein yield and inexpensive production costs. The large availability of well-characterised host strains coupled with extensive customized expression vectors can provide manufacturers with functional protein of high yields.
However, these microbial expression systems generally lack the ability to perform human-like PTMs. Prokaryotic systems have difficulties in forming disulphide bonds and many species, such as
Waker and collaborators identified the native glycosylation capability in
A research team from Cornell University have used a bottom-up engineering approach to assemble a synthetic glycosylation pathway within
(where Yaa, Xaa are not Pro 
|Sugar Moiety transferred to Asn||(GalNAc5GlcBac)||Glc3Man9GlcNAc2|
|Further sugar moiety processing after transfer||Not Observed||Yes|
|Lipid carrier||Undecaprenyl pyrophosphate (Und-PP)||Dolichol Phosphate (Dol-P)|
|oligosaccharyltransferase||One protein (subunit)||Greater than eight different subunits|
|Sugar Moiety Transfer Location||Periplasma||ER|
|Protein State on Transfer||Folded||Unfolded|
Genetic engineering of
Recently steps have been taken to genetically engineer humanized N-glycosylation pathways in non-mammalian eukaryotic expression systems such as yeast (
The N-glycosylation pathway in insects is more similar to mammalian eukaryotic species than
There are several examples of glycoengineered lepidopteran insect cell lines [135-138]. Specifically, a research group from University of Wyoming have transformed Spodoptera frugiperda (Sf9) cells with higher eukaryotic genes encoding for N-glycosylation pathway including gene products to enable terminal sialylation [136, 139]. Unlike previous glycoengineered species, which have incorporated genes into the genome, the glycosylation genes are placed under inducible plasmids in order to reduce metabolic overload, negative impact on growth rate, and long-term instability . The mammalian glycosylation genes are present on three vectors encoding six mammalian genes in total. These plasmids were transformed into Sf9 cell line which resulted in the sialylated N-glycosylation of a glycoprotein.
|Product Name||Manufacturer||Description||Expression System||Eurkaryotic or Prokaryotic|
|GlucaGen®||Novo-Nordisk||Glucagon for acute hypoglycaemia||Saccharomyces cerevisiae||Eurkaryotic|
|HEPLISAV™||Dynavax Technologies||Hepatitis B vaccine#||Hansenula polymorpha||Eurkaryotic|
|Engerix-B||GlaxoSmithKline||Hepatitis B vaccine||Saccharomyces cerevisiae||Eurkaryotic|
|KALBITOR®||Dyax Corp.||Inhibitor of the protein kallikrein in treatment of hereditary angioedema||Pichia Pastoris||Eurkaryotic|
|Humulin||Genentech/ EliLilly||Human Insulin||Escherichia Coli||Prokaryotic|
3.3. Manufacturing conditions
From the discussion relating glycan moieties of IFN-β, EPO and both the antibodies’ Fc as well as the Fab region to
|Nutrient availability||mAb/human hybridoma: change in microheterogeneity when glucose is substituted by fructose, mannose and galactose||Differential efficiency with which the cells consume the different carbon sources |
|IFNγ/CHO site occupancy on at glucose<0.3mM||Low biosynthesis of UTP and glucosamine 6- phosphate [142, 143]|
|IFNγ/CHO sialylation and high mannose glycans at 0.35mM <glucose<0.7mM and glutamine at 0.1mM||Decline of UDP-GlcNAc synthesis |
|Dissolved O2||Effect depends on cell line and product||EPO-Fc/CHO: no effect between 10-90% DOT, sialylation at 100% DOT||Mechanism not defined |
|hFSH/CHO: maximum sialylation at 100%DOT||Mechanism not defined |
|IL-2 glycovariant/BHK-21: no effect at 0% DOT||Mechanism not defined |
|IgG1 mAb/murine hybridoma CC9C10: galactosylation with DOT||UDP-Gal increases with DOT due to increased flux through glycolysis |
|Ammonia and pH||The effect between NH4+ and pH is synergic||mPL-I/murine: site occupancy on at 9mM NH4+||Mechanism not defined |
|TNF-IgG/CHO: 40% in galactosylation and sialylation||pH inhibits GalT and SiaT activities. pH causes GnTI, ManII, GalT and SiaT to mislocalize in Golgi [150-152]|
|IL-2/BHK-21: branching in at 15mM NH4+||Concentration of UDP-GlcNAc and UDP-GalNAc reduces the concentration of UDP-Ga l|
|EPO/CHO: sialylation of at 10mM NH4+||Concentration of UDP-GlcNAc decreases transport of CMP-Neu5Ac into Golgi [154, 155]|
|mPL-I/murine: 28% sialylation at 6.8<pH<7.8||pH inhibits GalT and SiaT activities. pH causes GnTI, ManII, GalT and SiaT to mislocalize in Golgi |
|Highest levels of IgG3 mAb/murine hybridoma galactosylation at pH=7.4, lowest levels of sialylation at pH=6.9||pH inhibits GalT and SiaT activities. pH causes GnTI, ManII, GalT and SiaT to mislocalize in Golgi |
|Viability, growth phase and temperature||EPO/CHO: branching at 32°C<T<37°C||temperature decreases UDP-GlcNAc and UDP-GalNAc concentration |
|Serum and lipid supplements||IFNγ/CHO: branching with lipid supplementation||Mechanism not understood because serum composition is unknown |
|IgG1/CHO: sialylation with serum-free medium||Mechanism not understood because serum composition is unknown |
|IL2/BHK-21: fucosylation and sialylation with serum-free medium||Mechanism not understood because serum composition is unknown |
|mAb/murine hybridomas: broader glycan distribution and galactosylation with BSA supplementation||Mechanism not understood because serum composition is unknown |
|pCO2 and osmolality||IgG2/SP2/0: unaffected up to 250mmHg pCO2|
galactosylation at 320mOsm/kg and high pCO2
25% in galactosylation at 195mmHg pCO2 and 435mOsm/kg
|osmotic stress induces pH which inhibits GalT and SiaT activities and causes them to mislocalize |
|Stirring speed||tPA/CHO: 72% site occupancy at 40<S<200rpm||Shear stress increases protein syhtnesis wich reduces ER retention time |
|Culture modes||I IFNγ/CHO: low dilution rates yield low site occupancy|
|Concentration of metabolites inhibits GalT and SiaT activity and mislocalizes these compounds. |
From Table 3, it is evident that many process parameters influence protein N-linked glycosylation. These effects could initially be seen as potential sources for variability. However, if their underlying mechanisms were to be fully understood quantitatively, they could serve as variables for the modulation and control of glycosylation-associated quality attributes of therapeutic proteins.
3.4. Medium formulation
When formulating media for mammalian cell culture for the production of secreted glycoproteins, the carbon source is of paramount importance. The rate at which glycosyltransferases can process glycans is subject to changes in substrate concentrations. The substrates of the glycosyltransferase reactions are the antibodies as well as nucleotide sugar donors (NSD). In the case of biotherapeutics, the important NSDs are UDP-GlcNAc, UDP-Gal, GDP-Fuc CMP-NeuAc and CMP-NeuGc as well as UDP-GalNAc when murine cell lines are employed over CHO cells. High levels of CMP-NeuGc terminating carbohydrate moieties are unfavorable in a therapeutic context as they are oncofetal and potentially immunogenic [163, 164]. The metabolism pathways and biosynthesis of sugars in mammalian cells are well known and are graphically represented in Figure 5 where the transport into the Golgi is also indicated. Expanding on this, the glycoform can be controlled and the concept of feeding strategies is based on the hypothesis that addition of specific metabolic intermediates of the nucleotide-sugar biosynthesis to culture medium will drive metabolic flux towards the desired NSD and eventually influence the glycoform through the increase of desired rates of reaction . Attention must be paid though to a number of inhibitory mechanisms which naturally regulate the depicted metabolic network.
In contrast to supplementing the culture medium with glycosylation substrates to increase certain reaction rates, other strategies have consisted of adding glycosylation reaction inhibitors to achieve desired glycoforms. More specifically, non-reactive fucose analogues have been added to mammalian culture medium to avoid core fucosylation of mAb Fc glycans . Others have added mannosidase inhibitors to prevent mAb Fc oligosaccharides form reaching more processed states .
3.5. In-process analysis of glycoproteins
In general, the complete analysis of oligosaccharide structures and linkages requires a series of steps; for example, enzymatic fragmentation, chromatographic separation steps and either mass spectrometry (MS) or nuclear magnetic resonance spectroscopy (NMR) to determine the chemical structure of the fractions . The technology for these offline analyses has improved in terms of the amount of material required, the sensitivity of the method, and the speed of analysis, but there is still room for improvement in miniaturization, speed, and throughput when it comes to analytics for bioprocessing. Particularly if analyses are going to be used to inform manufacturing operations in real-time, then faster methods with lower sample requirements will be required. Preferably, these should also be automatable to reduce the requirement for human expertise.
Early attempts at the high throughput analysis of post-translational modifications included a microtitre plate-based assay, where a series of steps including capture, desalting and reduction on beads, followed by elution, tryptic digestion, fractionation, and isolation of N-linked glycopeptides were performed in low volume before MALDI-TOF MS was used to identify structures . While this method had the advantage of small sample volume, it still was a lengthy procedure and while technically automatable, would be difficult to implement in-process.
One of the most important advances is the introduction of online methods for product analysis which will eventually enable real-time or quasi-real time control over the bioreactor environment in order to influence the product quality. Towards this end a number of new systems have been developed usually based on automated sampling followed by desalting via HPLC and analyses that have significantly shorter timescales from sampling to information.
For example,a two-dimensional system capable of analyzing up to 6 fractions from a separations operation was recently reported. Using a single HPLC system capable of running two columns simultaneously with independent gradients and switching between columns coupled to ESI-MS, fractions from size exclusion or ion exchange chromatography steps were analyzed for charge heterogeneity and size variation. An online concentration step was used to analyse dilute fractions to elucidate minor size variants. While unable to give complete peptide mapping of glycoforms, this represents a first step towards monitoring some of the important QbD properties online including glycoform heterogeneity and N-cyclisation . In another recent report, a method for the rapid detection and differentiation of sialic acids by HPLC was developed that is capable of analysing the content of
In a further example, Mittermayr et al, made a serendipitous discovery which might lead to more rapid sample processing times when analysing glycans from the Fc region of mAbs. They initially sought to compare the analysis capabilities of a newly developed hydrophilic interaction chromatography method with capillary electrophoresis coupled to laser induced fluorescence analysis, but found that the techniques were actually highly complementary in their resolving power. Thus, using a combination of the two in a 2D analysis, separation time for each could be reduced to 20 minutes. However, some very similar structures could not be resolved from each other, making this method incomplete .
Since measuring glycoform profile in real-time will remain a challenge in the near future, another approach is to measure surrogate markers that correlate with the glycan structure (and ultimately make a link using metabolic modelling). This idea is very similar to finding biomarkers for diagnostic indicators for disease—in essence, one or more ‘biomarkers’ for particular glycan structures of interest (e.g. high mannose, highly branched, or high levels of sialic acid endcapping) should exist. Once these are identified a fluorescent,
Also interesting are a suite of tools developed initially for glycomic analysis of whole cell samples (often in the context of disease investigations), but which might be adaptable to monitoring proteins secreted during bioprocessing.
One of the most promising of these techniques is the lectin microarray. Lectins are naturally occurring carbohydrate binding proteins which show some degree of specificity and have been shown to detect glycoproteins in less than 1 pg amounts . Previous work has used lectins conjugated to chromatography resin (e.g ) or to magnetic beads for recovering glycan containing proteins from a complex mixture, for example serum . The process can be done in small volumes using microtitre plates and the glycoproteins can later be analysed by MS in a process that can be automated by the use of liquid handling apparati .
Lectins immobilised to beads and fluorescence quenching of quantum dots have also been used to quantify specific types of glycoforms, suggesting a proof-of-principle for lectin based analysis . However, the main issue with lectin-based detection as a standard is that the specificity of individual lectins is broad, and therefore, specific detection of individual glycoforms is currently not possible. However, the possibility of using protein engineering or synthetic biology techniques to evolve panels of lectins with precise and varying specificity exists and could result in a platform amenable to high throughput in-process detection .
3.6. Experimental strategies for cell line modification
Another strategy to reduce glycan heterogeneity is genetic modification of cell lines. This can be through the overexpression or knockout of the genes encoding for glycosyl transferase enzymes. Such attempts aim to produce mAbs with specific biological functions or to avoid the expression of mAbs with glycan structures that are potentially immunogenic to humans. Umaña’s group overexpressed
Chaperone engineering can be another key parameter in boosting productivity. ER chaperones are responsible for correct protein folding and co-overexpression of chaperone genes and other regulatory elements (e.g. ERp57, calnexin/calreticulin, and/or protein disulfide isomerise) exhibited position effects in Productivity. Disulfide isomerase in particular increased specific mAb productivity by 55% in transient gene expression, but there were no/negative effects in stable gene expression . In addition, targeting the unfolded protein response (UPR) pathway is another approach to enhancing recombinant protein yield. The spliced form of X-box binding protein 1 (XBP-1s) is the spliced form of the parental XBP-1 protein and only exists upon the induction of ER-stress. Studies showed that overexpressing XBP-1s in mAb expressing-CHO-T cells under hypothermic condition increased total mAb concentration by 36 % .
In silicoprotein glycosylation studies
Mathematical modeling is a powerful tool for
The first mathematical investigation of glycosylation microheterogeneity was carried out by Umaña et al. in 1997 as part of a study into the effect of glycosyltransferase overexpression in a mammalian cell line in order to exert control over the glycoform . As part of this study, a
Krambeck and Betenbaugh extended the above described work through the inclusion of further glycosyltransferases and thus, expanding the number of structures from 33 to 7,565 structures resulting from a total of 22,871 reactions, which accounts for core fucosylation, galactosylation and sialylation of carbohydrate moieties . The model was also not specific to a single glycan site and viewed the Golgi compartments as four CSTRs in series. Building on previous work, enzyme dissociation constants from experimental investigations were employed for each glycosyltransferase and competitive product inhibition was taken into account. Furthermore, the model was evaluated and fitted against experimental data, where the glycoform data was obtained from recombinant human thrombopoietin (TPO) in which an average of 5.4 occupied N-glycan sites have been reported per molecule . Model optimisation was based on an averaged TPO glycan site, where enzyme concentrations were altered to give a closest fit to experimental data. Krambeck and Betenbaugh argue that while dissociation constants and kinetic data for the glycosyltransferases exist in literature, the enzyme concentration in the Golgi is cell line-dependent and will be subject to change based on culture conditions. Golgi-resident enzyme concentrations were changed to match data and resulted in improved model simulation results.
Krambeck et al. extended the model further in a follow up study, where the model was tailored to analyse glycoforms from mass spectrometric data . Mass spectrometry is based on mass to charge ratio of species and, thus, will not be able to distinguish between different structures of same molecular mass. The presented model attempts to resolve the issue through the prediction of alternative structures content for the same mass spectrometric data peak and eventually screen for glycan disease markers in humans. The model was extended through the addition of further glycosylation enzymes to makeup a total of 19 glycosyltransferases and subsequently enzyme activities were adjusted to match normal and malignant human monocyte N-glycan mass spectra. Through application of limiting conditions based on prior knowledge, glycans of probable negligible abundance can be omitted resulting in a total of 10,000 – 20,000 structures. The model gives valuable insight into changes in enzyme activity as a result of different diseases, but is rather limited in its application to the development and production of biotherapeutics, where large specificity with respect to the glycan structure and accessibility to the protein backbone is required for a highly accurate predictive model.
Hossler et al. attempted to improve the predictive ability of glycan distribution models through the variation of reaction-related variables . This model was the first to discriminate between reaction mechanisms for different glycosylation enzymes. ManI and ManII were modelled assuming Michaelis-Menten kinetics with substrate competition; the remaining transferases were modelled using a rapid equilibrium, random, Bi-Bi mechanism. While all previous models assumed the vesicular transport regime, which is modelled as a series of CSTRs, this study explored the hypothesis of the Golgi maturation model, which states that each compartment undergoes a maturation process to transform from early cisternae to late cisternae. In an idealised case this can be described by a plug flow reactor (PFR) and, thus, travelling through a tubular reactor, representing the Golgi cisternae. Modelling the Golgi apparatus as a single reactor of constant enzyme concentration showed that a long enough residence time will lead to highly processed glycan structures and changes in glycosyltransferase concentration can lead to a targeted glycoform for most glycan species. However, the authors of the study argued that a total of four reactors will be required to accurately simulate changes in the enzyme concentration along the length of the Golgi apparatus. The results show a greater appearance of under-processed glycan structures in the final product and generally less deviation from the data obtained from the CSTR-in-series model. A decrease in protein residence time was shown to have a much larger impact on the four PFR model than the CSTR model, with many more under-processed glycans were observed for the PFR case. It was further shown that modifications of the enzyme concentrations for the PFR-in-series model could lead to the most targeted glycoform and thus, demonstrating enzyme localization to be a very potent approach in glycan engineering. Hossler et al. concluded that while the actual biological mechanism will be less idealised than assumed in the study, the PFR-in-series model will give a more true distribution as demonstrated by comparison with experimental data.
Recently, Jimenez del Val et al. developed a model specifically tailored to the glycan found on the Asn297 position of an IgG antibody constant region . The model includes a cisternal maturation approach and expands on rate expressions for various enzymes to include Michaelis-Menten, sequential Bi-Bi and random order Bi-Bi kinetics for specific glycosyltransferases as reported in previous literature for each enzyme. The model considers the Golgi apparatus to be a single PFR of constant diameter, no axial dispersion within the compartment, constant flow and no mass transfer limitation where enzyme recycling along the biological reactor length leads to changes in glycosyltransferase concentrations. As a result it proposes a novel representation of enzyme concentrations along the length of a PFR as normal distribution functions. The unknowns of the three parameter normal functions for the spatial distribution of the enzymes where found through optimization-based methods, where the minimum amount of total enzyme necessary to achieve terminal oligosaccharide processing, including 50% sialylation, was sought. A further extension of previous mathematical models was the incorporation of protein-mediated nucleotide sugar donor transport into the Golgi cisternae. Again, due to the assumption of Golgi-resident protein recycling, a distribution of transport proteins is expected along the length of the PFR, which was estimated using an optimisation-based method. The optimisation was based on the assumption that the rate of by-product dephosphorylation is much faster than nucleotide sugar donor accumulation and, therefore, parameter values for minimum transport protein concentration were determined. Further, it was argued that while the above distributions should not change significantly, the dissociation constants will differ for individual glycoproteins as well as glycan sites within a glycoprotein. This accounts for steric hindrance and much reduced sialylation in the antibody Fc region and, hence, by taking dissociation constants for various commercial mAbs from literature, a glycoform was obtained. A comparison with experimental data, the Krambeck and Bettenbaugh, as well as the Hossler et al. model showed that the hereby obtained mathematical tool presented the closest fit to experimental data for most glycan species analysed as shown in Figure 6. Furthermore the model was demonstrated to show good fit to experimental data under gene silencing as presented in Figure 7.
5. Conclusions and outlook
The main goal of QbD is to ensure product quality by building it into the manufacturing process. The initiative provides practical incentives, such as shorter approval times and higher flexibility towards changes in manufacturing process conditions, for industrial production. Despite the volume of data required for QbD, the benefits far outweigh the effort. The first goal of QbD for biopharmaceuticals should be to narrow the glycomic profile of glycoprotein-based drugs based on existing knowledge of the desired structures for the application at hand and of the effect of manufacturing conditions and media/feed formulation on the availability of nucleotide sugars and the resulting glycan profile. This can be achieved more effectively through the combination of fundamental biological techniques for cell engineering, methodologies allowing rapid glycan analysis (in particular
Rapid analytical tools will allow us to examine more samples in-process with the aim of controlling and potentially optimising conditions in real time. An enabling tool is mathematical modelling, which, given its tremendous progress in successfully simulating the modification of complex glycoproteins, could in the future allow us to collect in process information about a fermentation run arising from various analyses at line and use it to infer the current state of the system and design improved operation strategies in terms of supplementation of nutrients or precursors, or adjustment of DOT, culture pH or other key conditions.
At the same time, while CHO cells clearly remain the dominant host system, research on other, more prominent cell lines that provide better glycosylation, or higher yield remains active. Yeast, insect, plant cells, and transgenic animals are amongst the more likely host systems to replace CHO cells. However, a significant amount of research effort regarding further bioprocess optimisation, as well as how they can be engineered to produce complex therapeutic molecules, such as heparin is still ongoing for CHO cells. Given the continued investment in CHO research, the number of existing production platforms, and pending patents as well as the stringency of the pharmaceutical regulatory agencies, they will likely remain a relevant industrial production host for at least another few decades.
That being said, the ideal expression system would achieve a high level expression of recombinant protein at a low cost. This implies that microbial hosts would lead to a significant cost advantage. Advances in humanising the PTM machinery in microbial hosts,