In eukaryotes, pathways concerned with protein synthesis and those involved in protein degradation serve to maintain the levels of proteins in a cell . The degradation of proteins occurs by two major pathways, the proteasomal degradation pathway and the lysosomal degradation pathway . In the proteasomal degradation pathway, target proteins are ubiquitinated by a system of E1, E2 and E3 enzymes [2,3]. Thereafter, the ubiquitinated proteins are delivered to the proteasome for degradation [2,3]. In contrast, the lysosome, which contains many hydrolytic enzymes, serves as the site of degradation for a multitude of pathways. One such pathway is the macroautophagy pathway . This undiscerning catabolic process, comprising of approximately 30
Vesicular transport facilitates the delivery of proteins to the different organelles of the cell, with the exception of transport to the nucleus, peroxisomes, endoplasmic reticulum etc . These intermediate carriers of proteins range from the endosomes to the coat protein complex I (COPI) vesicles, COPII vesicles and clathrin-coated vesicles [14-16]. The anterograde transport (from ER to Golgi) of proteins is mediated by the COPII vesicles while the retrograde transport (from Golgi to ER) is mediated by the COPI vesicles [14-16]. In addition, the transport of proteins from the plasma membrane to the early endosomes, and from the Golgi to endosomes is facilitated by the clathrin-coated vesicles . In yeast, the organelle that is homologous to the mammalian lysosome is the vacuole [17-18]. This organelle is essential for cellular processes such as maturation of vacuolar resident proteins, protein degradation, and for osmoregulation . The transport of vacuole resident proteins into the vacuole is essential for the function of this organelle.For example, the Vps pathway transports carboxypeptidase Y (CPY) from the Golgi to the vacuole for maturation . This pathway enlists the involvement of approximately 40
Transport of proteins and organelles to vacuole can be affected by alterations in nutrient stimuli [5,21]. Upon starving
2. Regulation of gluconeogenic enzymes in yeast
FBPase is a suitable candidate for degradation studies for two reasons. First, expression of FBPase can be induced in response to specific stimuli [25-27]. And secondly, following glucose replenishment, FBPase is promptly degraded and exhibits a half-life of approximately 20-40 min. A key factor in targeting FBPase for degradation may be protein modification. To better illustrate this, it has been suggested that phosphorylation of FBPase may be a regulatory factor in this protein’s degradation . There is evidence that FBPase is phosphorylated at serine 11 and that this phosphorylation increases following glucose replenishment . Protein kinase A (PKA) and the Ras2 signaling pathway mediate phosphorylation of FBPase [29-31].
3. The site of degradation of gluconeogenic enzymes
The site of degradation of FBPase is dependent on the duration of starvation. From studies conducted by the Wolf lab, it has been demonstrated that following glucose replenishment, FBPase is inactivated by ubiquitination [32-35]. There is evidence that the N-terminal proline residue is essential for the polyubiquitination of FBPase following glucose replenishment . Thereafter, ubiquitinated FBPase is then delivered to the proteasome for degradation [32-36]. This is because the mutations in genes involved in the proteasome pathway such as
4. The vacuole import and degradation pathway
The gluconeogenic enzymes (FBPase, MDH2, Pck1p and Icl1p) are transported to the vacuole for degradation by a selective autophagy pathway [37-41]. This pathway is called the vacuole import and degradation (Vid) pathway. The genes involved in this pathway are cumulatively called VID genes [37-41]. For the purposes of characterizing this pathway, FBPase was selected as a marker for associated studies. By using a myriad of mutagenesis assays, our lab has identified many genes that play a role in the Vid pathway. For instance, mutants, created by subjecting cells to UV mutagenesis, have been studied for their ability to degrade FBPase. A colony blotting procedure was utilized to screen for mutants defective in FBPase degradation following glucose replenishment . The results from these experiments were further validated by performing pulse-chase experiments. It was determined that while FBPase was degraded with a half-life of 20-40 min in wild-type cells, mutants degraded FBPase with a half-life ranging from 120-400 min. Moreover, all vid mutations were recessive as these mutants were complemented for the FBPase degradation defect upon mating with wild-type cells.
Another strategy to identify genes involved in the Vid pathway was by transposon mutagenesis. For this strategy, a transposon-lacZ/LEU2 library was transformed into wild-type cells. These mutants were then screened for FBPase degradation defects a using colony blotting procedure . The identities of the mutated genes were ascertained by extracting the genomic DNA and the subsequent amplification of the nucleotide sequences adjoining the transposon insertion site via PCR. The product from the PCR was sequenced and analyzed using gene sequence alignment software from the National Center for Biotechnology Information (NCBI). Moreover, the degradation defect attributed to these mutants was confirmed by using yeast null mutants for the corresponding genes. Furthermore, the FBPase degradation phenotype was rescued upon transforming the corresponding VID genes into these mutants. The vid mutants are distinct from those affecting protein secretion (sec), vacuolar proteolysis (pep) and vacuolar protein sorting (vps). Upon studying the distribution of FBPase in cells of these mutants, it was inferred that the mutants can be classified into two categories. After replenishing cells with fresh glucose, some mutants depicted a more cytosolic staining of FBPase (Class A mutants) while other mutants showed FBPase to be distributed in punctate structures (Class B mutants) .
5. Vidvesicles: Intermediate carriers of the Vid pathway
From fractionation analysis, it was proposed that in the Vid pathway, FBPase was delivered to the vacuole for degradation via intermediate vesicles. This hypothesis was investigated by isolation and purification of FBPase-associated vesicles to near homogeneity . In this investigation, wild-type cells were shifted to glucose for 30 min at 22ºC and vesicles were purified. At this temperature, there is a delay in the delivery of FBPase to the vacuole . Following homogenization and subsequent centrifugation at 100,000 x g of cells, the intracellular organelles were separated by size via fractionation on a Sephacryl S-1000 column. Immunoblotting with antibodies against FBPase and organelle markers enabled in assessing the purity of the isolated FBPase-associated vesicles. FBPase was detected in two distinct peaks from the S-1000 fractionation . The first peak was enriched in both the vacuole membrane marker CPY and the plasma membrane marker Pma1p . Interestingly, the second FBPase peak was enriched in a number of intracellular organelle markers. These include markers for the ER (Sec62p), Golgi (Mnn1p), vacuole (CPY), mitochondria (cytochrome C), and the ER-derived COPII vesicles (Sec22p) . Owing to the enrichment of the second FBPase peak with numerous intracellular organelle markers, this peak was purified by further fractionation on sucrose density equilibrium gradients. From this fractionation, it was ascertained that FBPase was present in only one peak that corresponded to a density of 1.18 – 1.22 g/ml . As this density did not correspond to any of the above intracellular organelle markers, this indicated that FBPase might be contained in distinct intracellular structures. Upon examining the FBPase containing peak using electron microscopy, a uniform population of vesicles (35-50 nm in diameter) was observed .
An understanding of how the Vid vesicles facilitate delivery of FBPase to the vacuole is vital to understanding the degradation kinetics of the Vid pathway. As such, it was first hypothesized that if the Vid vesicles serve as intermediate carriers in the Vid pathway, then FBPase will be associated with these vesicles prior to their delivery into the vacuole. In that endeavor, studying the distribution of FBPase at 22ºC aided in examining the kinetics of FBPase association with Vid vesicles in wild-type cells . In this experiment, wild-type cells were replenished with fresh glucose for various times at 22ºC. It was determined that FBPase was associated with the Vid vesicle fraction at t=30 min and was then distributed in both the Vid vesicle and vacuole fractions by 60 min . Moreover, FBPase was associated with the vacuole by 90 min . These results indicate that glucose induces FBPase to be distributed in Vid vesicles and that this occurs prior to delivery of this protein to the vacuole. CPY, which was used as a control in this experiment, was not affected by glucose under these same conditions . In order to ascertain whether FBPase was sequestered into the lumen of the Vid vesicles, the vesicles were purified and then incubated in the presence or absence of proteinase K . The underlying principle of this assay is that FBPase that is sequestered into the lumen of the Vid vesicles will be unaffected by proteinase K digestion and that FBPase that is peripherally associated with the vesicles will be digested by proteinase K. It was determined that FBPase was stable when incubated with proteinase K, which indicated that this protein was sequestered in the lumen of Vid vesicles . Addition of 2% Triton X-100 to permeabilize the Vid vesicle membrane resulted in digestion of FBPase by proteinase K. Thus, a portion of FBPase is sequestered inside Vid vesicles. However, these observations do not rule out the prospect of low amounts of FBPase being associated with the vesicles peripherally .
6. The biogenesis and trafficking of Vid vesicles to the vacuole
Owing to the unique nature of the Vid vesicles, innumerable questions need to be answered. Questions ranging from elucidating the origin of the vesicles to characterizing the mechanism by which FBPase is sequestered are imperative for better understanding the Vid pathway. In addition, if Vid vesicles are intermediary carriers of cargo protein in the Vid pathway, the vesicles should contain proteins that are essential for the import of FBPase into the vesicles and also for transport of FBPase from the vesicles to the vacuole. In that endeavor, VID24 was characterized as a gene involved in the degradation of FBPase in the Vid pathway. This gene was identified by chromosomal walking .
The VID24 gene encodes a protein with a molecular weight of 41 kDa. Vid24p has been characterized as a peripheral protein that is distributed to the Vid vesicles . Under glucose starvation conditions, Vid24p is expressed at low levels in wild-type cells. Following glucose replenishment, Vid24p is detected at increased levels from 20 to 120 min. It has been suggested that glucose induces de novo synthesis of Vid24p as addition of cyclohexamide with glucose was determined to inhibit induction of this protein. Furthermore, during glucose starvation, Vid24p produced weak fluorescence upon studying the distribution of Vid24p by immunofluorescence microscopy. In contrast, Vid24p produced a stronger fluorescent signal following glucose replenishment for 30 to 60 min. Interesting, Vid24p was mostly distributed in punctate structures within cells. This suggested that Vid24p was associated with intracellular organelles, which were later determined to be the Vid vesicles. This indicates that Vid24p is a structural protein for the Vid vesicles. Furthermore, this also suggests that the vid24-1 mutant belongs to the Class B category of mutants that accumulate FBPase in punctate structures. The above results highlight the requirement of Vid24p for the transport of FBPase from the Vid vesicles to the vacuole for degradation.
The next question pertains to the origin of the Vid vesicles. It has been proposed that the Vid vesicles may be derived from existing organelles and that they may be synthesized in cells even prior to glucose replenishment. Investigations surrounding the origins of the Vid vesicles have been hindered by the fact that Vid24p is only induced following 20-30 min of glucose replenishment. Therefore, events detailing the biogenesis of Vid vesicles during the first 20-30 min of glucose replenishment are difficult to examine with Vid24p. To circumvent this issue, an alternative strategy was designed that entailed the screening of mutants that failed to form Vid vesicles. This strategy would facilitate in assigning functions to mutants that were involved in specific steps of Vid vesicle biogenesis. In this manner, it was ascertained that the UBC1 gene was required for Vid vesicle biogenesis . As a matter of fact, the rate of FBPase degradation was observed to decrease in the null mutant of UBC1. Moreover, there was a diminished import of FBPase into the Vid vesicle fractions in the ∆ubc1 mutant. As such, it could be inferred that in the ∆ubc1 strain, there is a decrease in the level of Vid vesicles. For instance, Vid24p levels were enriched in the pellet fraction that was representative of Vid vesicles in wild-type cells. However, Vid24p levels were diminished in the pellet fraction in the ∆ubc1mutant, indicative of an impaired production of the Vid vesicles. At present, the mechanism by which UBC1 is involved in the biogenesis of Vid vesicles has not been elucidated. Moreover, the formation of multi-ubiquitin chains has also been implicated in the degradation of FBPase in the Vid pathway. As such, yeast strains expressing the R48K/R63K ubiquitin mutant, which blocks multi-ubiquitin chain formation, resulted in inhibiting the degradation of FBPase in the Vid pathway. Interestingly, there was also a diminished amount of FBPase that was associated with the Vid vesicle fraction. Thus, these observations suggest that the UBC1 gene and the formation of polyubiquitin chains are involved in the biogenesis of the Vid vesicles.
Another question is to understand how FBPase is imported into the Vid vesicles. To elucidate this, an in vitro system was developed to investigate the sequestration of FBPase into isolated Vid vesicles in the presence of the wild-type cytosol . A wild-type strain in which the endogenous FBP1 gene had been deleted for used for this in vitro assay. The Vid vesicles were isolated from this strain by differential centrifugation. Thereafter, the isolated Vid vesicles were incubated with a defined amount of purified FBPase in a reaction mixture that also contained wild-type cytosol, ATP and an ATP regenerating system. Proteinase K was added to the reaction mixture to degrade non-sequestered FBPase after 20 min of incubation. It was determined that 20-40% of the purified FBPase was protected from proteinase K digestion in vitro. Interestingly, addition of 2% Triton X-100 to permeabilize the membrane facilitated in the digestion of FBPase by proteinase K. As such, it can be inferred that FBPase is imported inside Vid vesicles, and that this import requires ATP and cytosol. In addition, our lab has also identified the cytosolic heat shock proteins Ssa1p and Ssa2p as being required for the import of FBPase into Vid vesicles .
Vid22p is a plasma membrane protein that was also determined to regulate FBPase sequestration into the Vid vesicles indirectly via the action of Cpr1p . By using a transposon mutagenesis strategy, our lab identified the VID22 gene. Following its synthesis in the cytosol, Vid22p is then targeted to the plasma membrane in a manner that is independent of the ER-Golgi transport pathway. It was determined that the null mutant of VID22 inhibited the degradation of FBPase following glucose replenishment. Interestingly, FBPase was found to accumulate in the cytosol of the ∆vid22 mutant strain. This indicates that VID22 may be required for the import of FBPase into the Vid vesicles. It was ascertained that FBPase sequestration into the Vid vesicles was inhibited upon combining the ∆vid22 mutant cytosol with the wild-type Vid vesicles using in vitro analysis. However, the wild-type FBPase import phenotype was rescued by incubating the wild-type cytosol with Vid vesicles from the ∆vid22 mutant. From these experiments, it can be inferred that the ∆vid22 mutant may contain functional Vid vesicles but have a defective cyotosolic environment. It has been determined that Vid22p, through its role in regulating the levels of Cpr1p, influences the degradation of FBPase. This is supported by the fact that the levels of Cpr1p in total lysates are diminished in the ∆vid22 mutant when compared to that observed in wild-type cells. However, this defect that is attributed to the absence of the VID22 gene is rescued by the addition of purified Cpr1p in vitro or by overexpressing Cpr1p in vivo. As such, the Cpr1p protein, whose levels are regulated by Vid22p, directly promotes FBPase import into the Vid vesicles. At present, the mechanism by which Vid22p regulated Cpr1p levels has not been elucidated.
The peptidylprolyl isomerase cyclophilin A (Cpr1p) was identified as being required for the import of FBPase into Vid vesicles . This cytosolic protein serves as a receptor for the immunosuppressant drug cyclosporin A. Our lab identified Cpr1p owing to its role as a mediator for the Vid protein Vid22p. By fractionating the wild-type cytosol by purification using ammonium sulfate precipitation, Superose 6 and G75 sizing chromatography, and DEAE ion exchange chromatography, our lab was able to isolate and identify Cpr1p. The role of Cpr1p in the degradation of FBPase was determined by using the ∆cpr1 mutant strain. It was ascertained that in vitro FBPase import and the subsequent degradation of FBPase was inhibited in the null mutant of CPR1. Furthermore, it was determined that the sequestration of FBPase into the wild-type Vid vesicles was impeded by the cytosol from the ∆cpr1mutant. In contrast, import of FBPase into the Vid vesicles from ∆cpr1 mutants was not impaired when supplied with the wild-type cytosol. The role of Cpr1p in the involvement of FBPase import into the Vid vesicles was verified by adding increasing amounts of purified Cpr1p to an in vitro reaction mixture containing the Vid vesicles and cytosol from the null mutant of CPR1. A control experiment comprising of addition of BSA tothe in vitro reaction mixture containing the Vid vesicles and cytosol from the null mutant of CPR1did not stimulate FBPase import. This suggests that Cpr1p has a direct involvement in the import of FBPase into the Vid vesicles.
7. The Vid pathway merges with the endocytic pathway todeliver cargo to the vacuole
In order to facilitate a better understanding of the biogenesis of Vid vesicles, Vid vesicles were isolated, purified and interacting proteins or those serving as structural components were identified using MALDI analysis. Interestingly, constituents of COPI vesicles such as Ret1p, Ret2p, Sec21p and Sec28p were identified on purified Vid vesicles . As described previously, the COPI vesicles mediate transport of proteins from the Golgi to the ER [15,50]. It has been previously reported that COPI proteins have also been identified as components of endocytic compartments in both mammalian cells and in yeast [15,50]. Moreover, COPI proteins are involved in multivesicular body sorting in yeast and in endosomal trafficking in mammalian cells [15,50]. Our lab has demonstrated that COPI proteins associate with Vid vesicles . This suggests that the COPI proteins may play a role in FBPase degradation. The RET1, RET2, RET3, SEC26, SEC27, SEC21 and SEC28 genes encode the different coatomer proteins in yeast. With the exception of SEC28, all the other genes are essential. As such, the role of the essential COPI genes in FBPase degradation was studied using temperature sensitive mutants. Following glucose replenishment of the null mutant of SEC28 and the COPI temperature sensitive mutants, it was ascertained that FBPase degradation was impaired. Moreover, the ∆sec28 mutant and all of the temperature sensitive mutants of COPI genes inhibited the import of FBPase into the Vid vesicles. The ∆vam3 mutant served as a control in these experiments. The VAM3 gene encodes a vacuolar t-SNARE that mediates fusion of intermediary vesicles with the vacuole. As such, the ∆vam3 mutant blocks FBPase degradation following its import into the Vid vesicles. These results suggest that the COPI genes are required for the import of FBPase into the Vid vesicles. The above results were verified by studying the distribution of FBPase in COPI mutants using sucrose density gradients. It was determined that FBPase distribution was enriched in the cytosolic fractions in these mutants and its levels were diminished in Vid vesicle fractions when compared to the ∆vam3 mutant. Intriguingly, the FBPase distribution in COPI mutants was similar to that observed in the ∆ubc1 mutant. As these mutants inhibit the formation of Vid vesicles, this indicates that the COPI genes are also involved in Vid vesicle biogenesis. During glucose starvation, COPI proteins were observed to localize with the Vid vesicle marker Vid24p and the cargo FBPase. Interestingly, levels of COPI proteins in the Vid vesicle fractions displayed a transient increase and decrease following glucose replenishment. Furthermore, it was determined that COPI proteins associated with Vid24p forming a complex. This association was increased following glucose replenishment and was required for recruiting Vid24p to the Vid vesicles.
As the COPI genes have been previously reported to be involved in endocytosis in mammalian cells, it was important to determine whether endocytosis may be involved in our degradation pathway . As a preliminary study, the kinetics of the uptake of the lipophilic dye FM4-64 was examined under our growth conditions. In wild-type cells, after its internalization, the FM4-64 dye stains the endocytic compartments before finally staining the vacuole membrane. Interestingly, the uptake of the FM dye differed upon studying its distribution in mutants that inhibited the degradation of FBPase in the Vid pathway. While mutants such as ∆vph1 displayed large FM circular distributions, other mutants such as ∆vam3 produced small FM-containing circles. Having identified Sec28p (COPI subunit of coatomer) as a structural protein of Vid vesicles, the distribution of this protein was studied as a means to monitor Vid vesicle trafficking. Sec28p was distributed in punctate structures following glucose replenishment of wild-type cells for 20-30 min. Following glucose replenishment, it was observed that Sec28p was localized to FM-containing endosomes in wild-type cells. In contrast, Sec28p failed to localize to FM-containing structures in the ∆vam3 mutant. As such, it can be inferred that the VAM3 gene is required for the distribution of Sec28p to endosomes.
It has been previously determined that the UBC1 gene is required for the biogenesis of Vid vesicles. In the null mutant of UBC1, FBPase is enriched in the cytosol and levels of Vid vesicles are diminished. The trafficking of Sec28p was also studied using a ∆ubc1 mutant . It was postulated that if COPI genes are involved in Vid vesicle biogenesis, then COPI proteins such as Sec28p may be discerningly distributed to structural precursors of Vid vesicles in the ∆ubc1 mutant. Following glucose replenishment of the ∆ubc1 mutant, it was observed that at the earlier time points, Sec28p was distributed at compartments that were stained by the FM dye. However, at later time points, Sec28p was distributed to the FM stained vacuole membrane. These results suggest that the UBC1 gene is not required for the anterograde transport of Sec28p to the vacuole. As such, it can be inferred that the step following the delivery of Sec28p to vacuole membrane may require UBC1. It has been previously established that the biogenesis and budding of the COPI vesicles requires the assembly of COPI proteins at the budding site. Therefore, mutations of the COPI genes should result in altering the distribution of Sec28p to sites where the COPI vesicle buds from a precursor structure. Similarly, it was hypothesized that as Sec28p is a structural component of Vid vesicles, mutations of other COPI genes should affect the distribution of Sec28p to sites where the Vid vesicle is formed. To test this, the distribution of Sec28p was examined in a ret2-1 mutant. In this mutant, the ret2-1 gene encodes for a temperature sensitive protein which comprises the δ subunit of the COPI complex. Shortly after glucose replenishment, it was determined that Sec28p localized to FM containing endosomes in the ret2-1 mutant. Interestingly, by 180 min following glucose replenishment, while FM had stained the vacuole membrane, Sec28p was observed as punctate dots near or on the vacuole membrane. This suggests that either Sec28p is a component of vesicles that are in the process of fusing with the vacuole or that Sec28p is budding from the vacuole as a component of retrograde vesicles. This was clarified by studying the distribution of Sec28p after pre-labeling the vacuole membrane with FM dye in the ret2-1 strain. It was ascertained that Sec28p was distributed to buds that were forming on the vacuole membrane following glucose replenishment. Based on our results, it can be inferred that Sec28p containing vesicles are involved in both transport to and from the vacuole.
8. Early steps of endocytosis and actin polymerization are required for degradation of cargo to the Vid pathway
It has been previously ascertained that the Vid pathway merges with the endocytic pathway. An elucidation of this site of merger would afford a better understanding of the Vid pathway. According to one postulation, the Vid vesicles may originate from the plasma membrane or the vacuole. Alternatively, Vid vesicles may converge with endocytic vesicles that are forming on the plasma membrane. This may suggest that FBPase is also distributed near the plasma membrane. This was studied by examining, at the ultra-structural level, the distribution of FBPase in wild-type and pep4 strains . In these studies, following prolonged glucose starvation, the yeast strains were replenished with media containing fresh glucose for 20 min. Immuno-electron microscopy using affinity purified FBPase antibodies followed by secondary antibodies conjugated with 10 nm colloid gold particles facilitated in visualizing the FBPase distribution (Figure 1). It was determined that in both wild-type and pep4 strains, a significant percentage of FBPase was distributed in irregularly shaped intracellular structures in the cytoplasm following glucose replenishment. Interestingly, FBPase was also found near the plasma membrane. This suggests that the early steps of the endocytic pathway are involved in the vacuole dependent degradation of FBPase. These irregularly shaped intracellular structures (containing FBPase) were purified by high speed centrifugation and passing the re-suspended pellet over a S-1000 column. In this manner, it was ascertained that these intracellular structures were enriched for the Vid vesicle marker Vid24p and the endosomal marker Pep12p. From this, it can be inferred that following glucose replenishment, Vid vesicles may associate with the endosomes to form large aggregates of FBPase containing structures.
Owing to the distribution of FBPase near the plasma membrane, this suggests that the early steps of endocytosis may be required for the Vid pathway. In yeast, it has been previously ascertained that the early steps of endocytosis is facilitated by actin polymerization [52-63]. Proteins involved in actin polymerization are recruited to the plasma membrane in a specific and orderedsequence (Figure 2). At the site of cargo internalization, coat module proteins and nucleation promotion factor (NPF) module proteins are recruited at the same time for shaping the membrane and for regulating actin assembly. Coat module proteins comprise of Sla1p, Lsb3p, Pan1p, and End3p. The NPF module proteins consist of Las17p, type I myosins Myo3p and Myo5p, and Vrp1p, Bzz1p and Bbc1p. With the exception of the type I myosins, it should be noted that the coat module proteins and the NPF module proteins are recruited independent of F-actin. Thereafter, the actin module proteins (consisting of 20 proteins) are recruited by F-actin to sites of actin assembly. The actin module proteins are involved in the organization and dynamics of the actin network. This module comprises of proteins such as Act1p, Arp2/3 protein complex, Abp1p, Cap1p, Cap2p, Sac6p and Aim3p among others. The Arp2/3 protein complex is involved in the nucleation of branched actin filaments. This protein complex is comprised of Arp2p, Arp3p, Arc15p, Arc18p, Arc19p, Arc35p and Arc40p. Additionally, the Las17p, Pan1p and Abp1p proteins are required for the activation of the Arp2/3 complex. Finally, the amphiphysin module proteins are recruited by F-actin to mediate scission of endocytic vesicles. This module comprises of Rvs161p and Rvs167p.
Under our growth conditions, upon examining the distribution of FBPase in a end3 strain, it was determined that the distribution of FBPase in the plasma membrane, endosome and Vid vesicle fractions was diminished in comparisonto the control vph1 mutant . This indicates that the early steps of endocytosis may be required for the association of FBPase with Vid vesicles. As it has been previously reported that actin polymerization facilitates the scission of endocytic vesicles from the plasma membrane, the degradation kinetics of FBPase were examined in mutants that blocked the different steps of actin polymerization. In this manner, it was ascertained that the null mutants of END3 and SLA1 served to inhibit the degradation of FBPase. Thus, it can be inferred that the actin polymerization genes are required for the association of FBPase with Vid vesicles. Next, fluorescent analysis was used to examine the distribution of proteins to actin patches (sites of actin polymerization). During glucose starvation, it was ascertained that there was a low percentage of co-localization of FBPase to actin patches in wild-type cells (Figure 3) [51,64]. Following glucose replenishment for up to 30 min, FBPase produced a high percentage of co-localization to actin patches. Interestingly, after 60 min of glucose replenishment, FBPase showed less co-localization to the actin patches. The distribution of MDH2 to actin patches also produced similar results. This indicates that the cargo proteins of the Vid pathway are targeted to the sites of actin polymerization on the plasma membrane.
The distribution of the Vid24p to actin patches was next studied in wild-type cells as a means to determine whether the Vid vesicles are distributed to actin patches (Figure 4) [51,64]. During glucose starvation and following replenishment for up to 30 min, Vid24p was observed to be co-localized with actin patches. Intriguingly, by the 60 min time point, Vid24p demonstrated less co-localization with the actin patches. The distribution of Sec28p to actin patches also produced similar results.This suggests that during glucose starvation and following replenishment for up to 30 min, Vid vesicles associate with actin patches. In addition, in the rvs167strain, there is a prolonged association of Vid24p and Sec28p with actin patches. As such, it can be inferred that the actin patches mediate the scission of the Vid-endocytic vesicles from the plasma membrane.
To summarize the above results, we assert that Vid24p and Sec28p are distributed at the sites of actin polymerization (involved in the early steps of endocytosis) during glucose starvation. The gluconeogenic enzymes, FBPase and MDH2 are sequestered into free Vid vesicles and Vid vesicles that are aggregated at the site of actin polymerization. Following the scission of Vid-endocytic vesicles into the cytoplasm as small Vid-endosomes, these vesicles cluster and form large asymmetrically shaped structures. Therefore, the Vid-endosomes serve as intermediary carriers of cargo destined for degradation in the vacuole.
9. The association of Vid vesicles and actin patches requiresVID30
As it has been previously determined that that the Vid pathway merges with the endocytic pathway, one could propose that association of Vid vesicles and actin patches may be a pivotal point of this integration. In that endeavor, the VID30 gene was identified as a putative candidate involved in the Vid pathway using a transposon library screen . This gene encodes a protein that has been previously reported to be involved in the proteasomal degradation of FBPase . Vid30p forms a complex with Vid24p and serves as an E3 ligase in the ubiquitination of FBPase. The requirement of VID30 in the Vid pathway was verified by examining FBPase degradation in both wild-type and vid30 cells . After glucose starvation for 3 days and following replenishment, FBPase was degraded in wild-type cells. In contrast, there was an inhibition of FBPase degradation in the vid30 cells. This indicates that VID30 is required for the vacuole dependent degradation of FBPase. In order to determine whether Vid30p was distribution to Vid vesicles, wild-type cells expressing Vid30p were glucose starved for 3 days followed by replenishment for up to 20 min. The cells were then subjected to differential centrifugation. Vid30p levels were enriched in the Vid vesicle enriched fraction. This infers that Vid30p is distributed to Vid vesicles.
Using pulldown assays, it was determined that Vid30p interacts with Vid24p and Sec28p under our growth conditions. Moreover, FBPase does not associate with this Vid30p-Vid24p complex. This further supports the notion that FBPase and Vid24p exist in topologically different environments. Thereafter, the effect of the absence of SEC28 on the interaction of Vid30p and Vid24p was examined using pulldown assays. In this study, Vid30p was pulled down and the levels of Vid24p was examined the bound and unbound fractions. In the sec28 mutant, the level of Vid24p in the bound fraction was diminished in comparison to that observed in wild-type cells. This indicates that Sec28p is required for the association of Vid30p with Vid24p. Furthermore, the absence of VID24 also resulted in diminishing the interaction of Vid30p with Sec28p.
The co-localization of Vid30p with actin patches was studied using fluorescent miscroscopy. In wild-type cells, it was ascertained that Vid30p was co-localized with actin patches during glucose starvation and following glucose replenishment for up to 30 min (Figure 5) . By the 60 min time point, the localization of Vid30p to actin patches began to diminish. In the absence of VID24, Vid30p co-localization with actin patches was prolonged following glucose replenishment (Figure 6) . The absence of SEC28 also prolonged the Vid30p co-localization to actin patches. This suggests that SEC28 and VID24 mediate the dissociation of Vid30p and actin patches. Interestingly, deletion of genes involved in the later steps of actin polymerization, such as RVS161, also resulted in prolonging the co-localization of Vid30p with actin patches.
Differential centrifugation was used to determine the step of the Vid pathway that requires the VID30 gene. Inthis study, wild-type and vid30 cells were glucose starved and replenished with glucose for 20 min. By differential centrifugation, it was determined that FBPase was detected in both the Vid vesicle and cytosolic fractions in vid30 cells. This is similar to what was observed for wild-type cells. Moreover, most of Vid24p was detected in the Vid vesicle fraction in the vid30 mutant. This suggests that Vid vesicle formation occurs in the absence of the VID30 gene. Even though Vid30p is distributed tomultiple compartments, the deletion of this gene has no impact on the levels of FBPase and Vid24p in the Vid vesiclefraction. Moreover, it was determined that FBPase (Figure 7) and Vid24p (Figure 8) failed to co-localize with actin patches in vid30 cells . This suggests that Vid30p is required for the association of Vid vesicles and actin patches.
Vid30p contains two domains, a LisH (lissencephaly type 1-like homology) and CTLH (C-terminal to the LisH) domain. It has been previously reported that the LIS1 gene is mutated in Miller-Dieker lissencephaly, a medical condition that contributes to retardation and premature mortality. In addition, the CTLH domain has been postulated to be involved in microtubule function. It was next determined if these domains play a role in the vacuole dependent degradation of FBPase. In either deletion of LisH or CTLH domain in the VID30 gene, FBPase degradation was inhibited. Deletion of either domain also resulted in diminishing the association of Vid30p with Vid24p, and also with Sec28p. Vid30p, in which either LisH or CTLH domain had been deleted, was observed to be distributed to Vid vesicles and actin patches.Unlike in wild-type cells, the mutant Vid30p failed to be distributed to the vacuole membrane and was observed to aggregate in punctate structures. Similarly, upon deleting either domain, FBPase was also observed to localize to punctate structures. This indicates that the LisH and CTLH domains of Vid30p are involved in the later steps of the Vid pathway. Thus, in summation, VID30 is required for the association of Vid vesicles and actin patches, and that the LisH and CTLH domains also required at a later step in the Vid pathway.
10. Proposedmodel for the Vid pathway
Based on the above findings, we postulate the following model for the vacuole import and degradation pathway (Figure 9). When Saccharomyces cerevisiae are grown under glucose starvation conditions, this induces synthesis of gluconeogenic enzymes such as FBPase, MDH2, Pck1p and Icl1p. Vid30p, Vid24p and Sec28p are present as a complex and are distributed on free Vid vesicles and on Vid vesicles aggregating around endocytic vesicles at the actin patch sites. Ubc1p has been implicated in the biogenesis of Vid vesicles. Moreover, Vid30p is required for the association of actin patches to Vid vesicles. In response to glucose, PKA facilitates in the phosphorylation of cargo proteins. Vid vesicles are distributed freely in the cytoplasm and also aggregate around the endocytic vesicles forming from the plasma membrane. The cargo proteins are sequestered into Vid vesicles and this step requires Vid22p and Cpr1p. Vid30p facilitates in the association of free Vid vesicles and actin patches. The amphiphysin module proteins (Rvs161p and Rvs167p) mediate the scission of Vid-endocytic vesicles and these are released as Vid-endosomes into the cytoplasm. Thereafter, the free Vid vesicles also accumulate around the Vid-endosomes to form large Vid-endosome clusters. The LisH and CTLH domains of Vid30p are required for the delivery of Vid-endosome clusters to the vacuole for degradation of cargo proteins. Overall, our current model highlights the association of Vid vesicles to actin patches as mediated by Vid30p as a crucial step in the degradation of gluconeogenic enzymes in the vacuole.
11. Future directions
Many questions remain to be answered concerning the degradation of cargo proteins by the Vid pathway. A pivotal question surrounding the mechanismthat regulates the degradation of gluconeogenic enzymes by the proteasomal pathway versus the Vid pathway requires further elucidation. Previously, differential modification of cargo proteins following glucose replenishment has been attributed to dictate the site of degradation. For instance, proteins that are degraded in the proteasome are ubiquitinated prior to their degradation. In contrast, cargo proteins that are degraded in the vacuole are subject to phosphorylation by PKA before degradation . Intriguingly, what signaling stimulus regulates this transition from degradation in the proteasome versus that in the vacuole? This warrants further elucidation.
A second concern pertains to the origin of Vid vesicles. According to one proposal, Vid vesicles may be derived from the plasma membrane or the sites of internalization. Alternatively, Vid vesicles may originate from the vacuole membrane as retrograde vesicles. Deletion of genes involved in plasma membrane internalization, such as rvs161 orrvs167, contributed in prolonging the association of Vid vesicles to actin patches . Perhaps Vid vesicles are components of endosomes. It is interesting to note that the Vid-endosome clusters that are formed following glucose replenishment share morphological similarities to multivesicular bodies. Moreover, the importance of the early steps of endocytosis and actin polymerization for the vacuole dependent degradation of cargo proteins requires further analysis. This could imply that cargo proteins are secreted out of the cells and then internalize at actin patch sites. As cargo proteins do not contain the ER-Golgi secretory signal sequence, this could facilitate in the understanding of the non-classical secretory pathway.
A comprehensive understanding of the Vid pathway could have significant implications in studying the etiology of diseases associated with abnormal gluconeogenesis in humans. And this could aid in developing therapeutics that regulate gluconeogenesis and treat the subsequent malady. For example, it has been previously reported that patients afflicted with Type II diabetes also suffer from an increase in levels of gluconeogenesis . And an FBPase inhibitor called managlinat dialanetil has proven to be relatively successful in the treatment of Type II diabetes . Another example is that FBPase may be attributed to cause clonorchiasis-associated hepatic fibrosis owing to the protein’s secretion along with excretory products from Clonorchis sinensis adult worms . Moreover, studies aimed at evaluating deterioration of the proximal renal tubules have identified FBPase as a crucial marker in this determination . As such, seeking answers to the above questions will enable us in the development of treatments that will help improve the quality of life for the general public.
Regulated protein degradation Varshavsky A. 2005Trends Biochem. Sci. 30 283 286
Goldberg AL 2007Functions of the proteasome: from protein degradation and immune surveillance to cancer therapy. Biochem. Soc. Trans. 35 (Part 1): 12-17.
Mortimore GE, Hutson NJ, Surmacz CA 1983Quantitative correlation between proteolysis and macro- and microautophagy in mouse hepatocytes during starvation and refeeding. Proc. Natl. Acad. Sci. USA 80 2179 2183.
Seglen PO, Gordon PB, Holen 1990(1990) Non-selective autophagy. Semin. Cell Biol. 1 441 448.
Mizushima N. Klionsky D. J. 2007Protein turnover via autophagy: implications for metabolism. 7 19 40.
Wang CW, Klionsky DJ 2003The molecular mechanism of autophagy. Mol. Med. 9 65 76.
Klionsky DJ 2005The molecular machinery of autophagy: unanswered questions. J. Cell Sci. 118 7 18.
Kaushik S. Cuervo A. M. 2008Chaperone-mediated autophagy. 445 227 244.
Massey A. Kiffin R. Cuervo A. M. 2004Pathophysiology of chaperone-mediated autophagy. Int. J. Biochem. Cell Biol. 36 2420 2434.
Cuervo AM, Dice JF 2000Age-related decline in chaperone-mediated autophagy. J. Biol. Chem 275 31505 31513.
Chiang, HL, Dice JF 1988Peptide sequences that target proteins for enhanced degradation during serum withdrawal. J. Biol. Chem. 263 6797 6805.
- 12. Mizushima N, Levine B, Cuervo AM, Klionsky DJ (2008)Autophagy fights disease through cellular self-digestion.Nature451: 1069-1075.
Cuervo AM 2004Autophagy: in sickness and in health. Trends Cell. Biol. 14 70 77
McMahon HT, Mills IG 2004COP and clathrin-coated vesicle budding: different pathways, common approaches. Curr. Opin. Cell Biol. 16 379 391.
Gu F. Aniento F. Parton R. G. Gruenberg 1997(1997) Functional dissection of COP-I subunits in the biogenesis of multivesicular endosomes. J. Cell Biol. 139 1183 1195
Duden 2003(2003) ER-to-Golgi transport: COP I and COP II function. Mol. Membr. Biol. 20 197 207.
Dunn WA Jr. 1990Studies on the mechanisms of autophagy: formation of the autophagic vacuole. J. Cell Biol. 110 1923 1933.
Bryant NJ, Stevens TH 1998Vacuole biogenesis in : protein transport pathways to the yeast vacuole. Microbiol. Mol. Biol. Rev. 62 230 247.
Jones EW 1991Three proteolytic systems in the yeast . J. Biol. Chem. 266 7963 7966.
Robinson JS, Klionsky DJ, Banta LM, Emr SD 1988Protein sorting in : isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol. Cell. Biol. 8 4936 4948.
Nakatogawa H. Suzuki L. Kamada Y. Ohsumi 2009, Ohsumi Y (2009) Dynamics and diversity in autophagy mechanisms: lessons from yeast. Nat. Rev. Mol. Cell. Biol. 10 458 467.
Wedaman K. P. Reinke A. Anderson S. Yates 33rd, McCaffery JM, Powers T (2003) Tor kinases are distinct membrane-associated protein complexes in . Mol. Biol. Cell 14 3 1204 1220.
Reinke A. Anderson S. Mc Caffery J. M. Yates 3M, Yates J 3rd, Aronova S, Chu S, Fairclough S, Iverson C, Wedaman KP, Powers T (2004) TOR complex 1 includes a novel component, Tco89 YPL180w, and cooperates with Ssd1p to maintain cellular integrity in . J. Biol. Chem. 279(15): 14752-14762.
Yuan W. Stromhaug P. E. Dunn W. A. 1999Glucose-induced autophagy of peroxisomes in requires a unique E1-like protein. Mol. Biol. Cell. 10 1353 1366.
Chiang HL, Schekman 1991(1991) Regulated import and degradation of a cytosolic protein in the yeast vacuole. Nature 350 313 318.
Gancedo 1971(1971) Inactivation of fructose-1,6-bisphosphatase by glucose in yeast. J. Bacteriol. 107 401 405.
1989olzer H (1989) Proteolytic catabolite inactivation in . Revis. Biol. Celular. 21 305 319.
Minard KI, McAlister-Henn 1992(1992) Glucose-induced degradation of the MDH2 isozyme of malate dehydrogenase in yeast. J. Biol. Chem. 267 17458 17464.
Mazon MJ, Gancedo JM, Gancedo 1982(1982) Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme. J. Biol. Chem. 257 3 1128 1130.
Rittenhouse J. Harrsch P. B. Kim J. N. Marcus 1986(1986) Amino acid sequence of the phosphorylation site of yeast () fructose-1,6-bisphosphatase. J. Biol. Chem. 261 9 3939 3943
Jiang Y. Davis C. Broach J. R. 1998Efficient transition to growth on fermentable carbon sources in requires signaling through the Ras pathway. EMBO J. 17 23 6942 6951.
Schork S. M. Thumm M. Wolf D. H. 1995Catabolite inactivation of fructose-1,6-bisphosphatase of . Degradation via the ubiquitin pathway. J. Biol. Chem. 270 26446 26450.
Schork S. M. Bee G. Thumm M. Wolf D. H. 1994Catabolite inactivation of fructose-1,6-bisphosphatase in yeast is mediated by the proteasome. FEBS Lett. 349 270 274.
Horak J. Wolf D. H. 2002Two distinct proteolytic systems responsible for glucose induced degradation of fructose-1,6-bisphosphatase and the Gal2p transporter in the yeast share the same protein components of the glucose signaling pathway. J. Biol. Chem. 77 8248 8254.
Mol. Biol. Cell Regelmann J. Schule T. Josupeit F. S. Horak J. Rose M. Entian K. D. Thumm M. Wolf D. H. (200 Catabolite degradation. of fructose-1,6-bisphosphatase. in the. yeast Saccharomyces. cerevisiae a. genome-wide screen. identifies eight. novel G. I. D. genes indicates the. existence of. two degradation. pathways 14 1652 1663.
Hammerle M. Bauer J. Rose M. Szallies A. Thumm M. Dusterhus S. Mecke D. Entian K. D. Wolf D. H. 1998Proteins of newly isolated mutants and the amino-terminal proline are essential for ubiquitin-proteasome catalyzed catabolite degradation of fructose-1,6-bisphosphatase of . J. Biol. Chem. 273 25000 25005.
Chiang HL, Schekman 1994(1994) Site of catabolite inactivation. Nature 369: 284.
Hung G. C. Brown C. R. Wolfe A. B. Liu J. Chiang H. L. 2004Degradation of the gluconeogenic enzymes fructose-1,6-bisphosphatase and malate dehydrogenase is mediated by distinct proteolytic pathways and signaling events. J. Biol. Chem. 279 49138 49150.
Chiang H. L. Schekman R. Hamamoto 1996chekman R, Hamamoto S (1996) Selective uptake of cytosolic, peroxisomal and plasma membrane proteins by the yeast vacuole. J. Biol. Chem. 271 9934 9941.
Hoffman M. Chiang H. L. 1996Isolation of degradation-deficient mutants defective in the targeting of fructose-1,6-bisphosphatase into the vacuole for degradation in . Genetics 143 1555 1566.
Brown C. R. Hung G. C. Dunton D. Chiang H. L. 2010The TOR complex 1 is distributed in endosomes and in retrograde vesicles that form from the vacuole membrane and plays an important role in the vacuole import and degradation pathway. J. Biol. Chem 285 30 23359 23370.
Egner R. Thumm M. Straub M. Simeon A. Schuller H. J. Wolf D. H. 1993Tracing intracellular proteolytic pathways. Proteolysis of fatty acid synthase and other cytoplasmic proteins in the yeast . J. Biol. Chem. 268 36 27269 27276.
Brown C. R. Mc Cann J. A. Hung G. Elco C. Chiang H. L. 2002Vid22p, a novel plasma membrane is required for the fructose-1,6-bisphosphatase degradation pathway. J. Cell Sci. 115 655 666.
Huang PH, Chiang HL 1997Identification of novel vesicles in the cytosol to vacuole protein degradation pathway. J. Cell Biol. 136 803 810.
Chiang MC, Chiang HL 1998Vid24p, a novel protein localized to the fructose-1,6-bisphosphatase containing vesicles, regulates targeting of fructose-1,6-bisphosphatase from the vesicles to the vacuole for degradation. J. Cell Biol. 140 1347 1356.
Shieh H. L. Chen Y. Brown C. R. Chiang H. L. 2001Biochemical analysis of fructose-1,6-bisphosphatase import into vacuole import and degradation vesicles reveals a role for in vesicle biogenesis. J. Biol. Chem. 276 10398 10406.
Brown CR, McCann JA, Chiang HL 2000The heat shock protein Ssa2p is required for import of fructose 1-6-bisphosphatase into Vid vesicles. J. Cell Biol. 150 65 76.
Brown CR, Cui DY, Hung GG, Chiang HL 2001Cyclophilin A mediates Vid22p function in the import of fructose-1,6-bisphosphatase into Vid vesicles. J. Biol. Chem. 276 48017 48026.
Brown C. R. Wolfe A. B. Cui D. Chiang H. L. 2008The vacuolar import and degradation pathway merges with the endocytic pathway to deliver fructose-1,6-bisphosphatase to the vacuole for degradation. J. Biol. Chem. 283 26116 26127.
Gabriely G. Kama R. Gerst J. E. 2007Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast. Mol. Cell Biol. 27 2 526 540.
Brown CR, Dunton DD, Chiang HL 2010The vacuole import and degradation pathway utilizes early steps of endocytosis and actin polymerization to deliver cargo proteins to the vacuole for degradation. J. Biol. Chem. 285 2 1516 1528.
Engqvist-Goldstein AE, Drubin DG 2003Actin assembly and endocytosis: from yeast to mammals. Annu. Rev. Cell Dev. Biol. 19 287 332.
Toret CP, Drubin DG 2006The budding yeast endocytic pathway. J. Cell Sci. 119 4585 4587.
Warren DT, Andrews PD, Gourlay CW, Ayscough KR 2002Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics. J. Cell Sci. 115 1703 1715.
Howard JP, Hutton JL, Olson JM, Payne GS 2002Sla1p serves as the targeting signal recognition factor for NPFX(1,2)D-mediated endocytosis. J. Cell Biol. 157 315 326.
Michelot A. Costanzo M. Sarkeshik A. Boone C. Yates J. R. I. I. I. Drubin D. G. 2010Reconstitution and protein composition analysis of endocytic actin patches. Curr. Biol. 20 1890 1899.
Boettner DR, Chi RJ, Lemmon SK 2012Lessons from yeast for clathrin-mediated endocytosis. Nat. Cell Biol. 14 1 2 10.
, Maldonado-Baez L, Davey M, Montpetit B, Schluter C, Wendland B, Conibear E (2009) Regulators of yeast endocytosis identified by systematic quantitative analysis. J. Cell Biol. 2009 185 6 1097 1110.
Liu J. Sun Y. Oster G. F. Drubin D. G. 2010Mechanochemical crosstalk during endocytic vesicle formation. Curr. Opin. Cell Biol. 22 1 36 43.
Kaksonen M. Sun Y. Drubin D. G. 2003A pathway for association of receptors, adaptors, and actin during endocytic internalization. Cell 115 4 475 487.
Michelot A. Drubin D. G. 2011Building distinct actin filament networks in a common cytoplasm. Curr. Biol. 21(14): R 560-R569.
Dewar H. Warren D. T. Gardiner F. C. Gourlay C. G. Satish N. Richardson M. R. Andrews P. D. Ayscough K. R. 2002Novel proteins linking the actin cytoskeleton to the endocytic machinery in . Mol. Biol. Cell 13 3646 3661.
Kishimoto T. Sun Y. Buser C. Liu J. Michelot A. Drubin D. G. 2011Determinants of endocytic membrane geometry, stability, and scission. Proc. Natl. Acad. Sci. U.S.A. 108(44): E 979 988.
Alibhoy AA, Giardina BJ, Dunton DD, Chiang HL 2012Vid30 is required for the association of Vid vesicles and actin patches in the vacuole import and degradation pathway. Autophagy 8 29 46.
Santt O. Pfirrmann T. Braun B. Juretschke J. Kimmig P. Scheel H. Hofmann K. Thumm M. Wolf D. H. 2008The yeast GID complex, a novel ubiquitin ligase (E3) involved in the regulation of carbohydrate metabolism. Mol. Biol. Cell 19 8 3323 3333.
Cui DY, Brown CR, Chiang HL 2004The type 1 phosphatase Reg1 Glc7pis required for the glucose-induced degradation of fructose-1,6-bisphosphatase in the vacuole. J. Biol. Chem. 279: 9713-9724.
Wang Y. Tomlinson 2007(2007) Managlinat dialanetil, a fructose-1,6-bisphosphatase inhibitor for the treatment of type 2 diabetes. Curr. Opin. Investig. Drugs 8 849 858.
Zheng M. Hu K. Liu W. Hu X. Hu F. Huang L. Wang P. Hu Y. Huang Y. Li W. Liang C. Yin X. He Q. Yu 2011, Hu F, Huang L, Wang P, Hu Y, Huang Y, Li W, Liang C, Yin X, He Q, Yu X (2011) Proteomic analysis of excretory secretory products from adult worms: molecular characterization and serological reactivity of a excretory-secretory antigen-fructose-1,6-bisphosphatase. Parasit. Res. 109 3 737 744.
Kepka A, Dariusz Szajda S, Stypulkowska A, Waszkiewicz N, Jankowska A, Chojnowska S, Zwierz K (2008) Urinary fructose-1,6-bisphosphatase activity as a marker of the damage to the renal proximal tubules in children with idiopathic nephrotic syndrome. Clin. Chem. Lab. Med. 46:831-835.