Various membranes for desalination.
Membranes from biological polymers are anticipated practical application as biocompatible materials in separation technology. Biological polymers produced from bioresources are expected to be environmentally compatible polymers and to have great potential as alternatives to various artificial polymers produced from petroleum.
The application of membrane separation in the food industry, medical devices, and water treatment has attracted the attention of biochemical engineering. Membrane separation processes effectively reduce energy cost and CO2 production. In addition, interest in using natural materials has increased, due to their biocompatibility and their lack of environment load upon disposal. Biopolymer membranes made of cellulose, [1-2], gelatin , and chitosan  have been anticipated for application in biocompatible separation processing.
1.1. Membrane Desalination
Desalination technology grows exponentially to support water supply from sea water. Today, three billion people around the world have no access to clean drinking water. By 2020, there will be a worldwide 17% short of fresh water needed to sustain the world population. Moreover, 1.76 billion people live in areas already facing a high degree of water stress [5-6].
Generally, desalination can be categorized into two major types: (1) phase-change/thermal process and (2) membrane-based process. Examples of the phase-change process include multi-stage flash, multiple-effect boiling, vapor compression, freezing humidification/dehumidification, and solar stills. Membrane-based processes include reverse osmosis (RO), nanofiltration (NF), ultrafiltration (UF), membrane distillation (MD), and electrodialysis (ED) . Membrane separation technology for desalination is expected to reduce energy consumption.
1.1.1. Artificial Polymer Membrane for Desalination
Previous studies on membrane desalination are listed in Table 1. Various artificial polymers exhibited excellent capability in separation engineering and practical application for desalination, dialysis, and water treatment [8-10].
|Hsu, S. T. et al.||2002||PTFE||MD||8|
|Haddad, R. et al.||2004||Cellulose||NF||18|
|Peng, P. et al.||2005||PVA/PEG||MD||9|
|Gazagnes, L. et al.||2007||Ceramic||MD||10|
|Miao, J. et al.||2008||Chitosan Polysulfone||NF||16|
|Padaki, M. et al.||2011||Chitosan Polypropylene||NF||17|
|Zhang, S. et al.||2011||Cellulose||FO||19|
|Papageorgiou, S. K. et al.||2012||Alginate||Photocatalytic UF||14|
|MD: Membrane distillation, NF: Nanofiltration, FO: Forward osmosis, UF: Ultrafiltration
PTFE: Polytetrafluoro ethylene, PVA: Polyvinyl alchohol, PEG: Polyethylene glycol
1.1.2. Biological Polymer Membrane
Alginate is a typical marine biopolymer used as a fouling model in the desalination field [11-12]. Recently, the high performance of desalination of the alginate membrane has been expected to provide highly efficient desalination because sensitive molecular screening characteristics of the alginate membrane have been demonstrated . In addition, alginate-based materials have been developed as support for photocatalysts. Papageorgiou et al. pioneered a hybrid photocatalytic/ultrafiltration process for treating water containing toxic organic compounds .
Chitosan has often been investigated for application in desalinating marine biological polymers. Chitosan membrane has strong antibacterial activity in a higher deacetylation degree . N,O-carboxymethyl chitosan and polysulfone composite membrane cross-linked with epichlorohydrin was recently developed .
At 293K and 0.40 MPa, the membrane rejected 90.4% of the Na2SO4 solution (1000mg L-1) while the permeate flux was 7.9 kg m-2 h-1 (Na2SO4). In contrast, the membrane rejected 27.4% of the NaCl solution while the permeate flux was 10.8 kg m-2 h-1 (NaCl). Polypropylene supported chitosan NF-membrane has also demonstrated good desalination ability in acidic pH .
Haddad et al. indicated that cellulose acetate nanofiltration (NF) could be adapted to desalination processes . Cellulose ester membrane was also investigated in forward osmosis (FO) for desalination . Forward osmosis has been applied worldwide in recent years as a novel alternative desalination technology for producing fresh water .
Alginic acid is abundantly produced by marine biological resources, especially brown seaweed. The first description of alginate as a preparation of “algic acid” from brown algae was provided and demonstrated by British chemist E. C. C. Stanford, with a patent dated 12 January 1881 . In 1896, A. Krefting successfully prepared a pure alginic acid. Kelco Company began commercial production of alginates in 1929 and introduced milk-soluble alginic acid as an ice cream stabilizer in 1934 .
Alginates have been conventionally applied in the food industry as thickeners, suspending agents, emulsion stabilizers, gelling agents, and film-forming agents .
Sodium alginate is a typical hydrophilic polysaccharide. It consists of a linear copolymer composed of two monomeric units, 1,4-linked β-D-mannuronic acid (Figure 1a) and α-L-guluronic acid (Figure 1b), in varying proportions. These two uronic acids have only minor differences in structure, and they adopt different chair conformations such that the bulky carboxyl group is in the energetically favored equatorial position .
The physical properties (e.g., viscosity and mean molecular weight) of sodium alginate are very susceptible to physicochemical factors (e.g., pH and total ionic strength). At near-neutral pH, the high negative charge of sodium alginates due to deprotonated carboxylic functional groups induces repulsive inter- and intra-molecular electrostatic forces. The change of ionic strength in a sodium alginate aqueous solution has a significant effect, especially on the polymer chain extension [25-27].
2.1. Chemical Formation
An alginate molecular chain was constructed using three types of polymeric block: homopolymeric blocks of mannuronic acid (M-M), guluronic acid (G-G), and blocks with an alternating sequence in varying proportions (M-G)  (Figure 1c).
The M-M block consists of 1e→4e linked β-D-mannuronic acid chains with the monosaccharide units in a 4C1 chair conformation. Regions in which β-D-mannuronic acid predominates have been predicted to form an extended ribbon structure, analogous to cellulose .
The G-G block is composed of 1→4 diaxially linked α-L-guluronic acid residues in a 1C4 chair conformation. It forms a buckled chain . The molecular construction of the G-G block has been confirmed experimentally by X-ray diffraction analysis of the partial hydrolysis products of alginate. The mass fraction of these blocks is basically derived from a natural species of brown algae. At present, the production of new tailor-made alginates has been prompted by the availability of C-5 epimerases, which facilitate extremely efficient tuning of both composition and physicochemical properties of the polysaccharide. In particular, the epimerase AlgE4, which enables the conversion of M-M blocks into alternating sequences in a processive mode of action , has provided new alginates with interesting properties. In this respect, besides the remarkable increase in syneresis displayed by the AlgE4 treated samples, a much higher stability of the gel is directly correlated with the presence of long alternating sequences [32-33].
2.2. Gelling Ability
Sodium alginate rapidly forms a gel structure with the presence of divalent cations such as Ca2+, resulting in a highly compacted gel network . Spherical gel particles of calcium alginate are often investigated and applied as a carrier of immobilized enzyme , a drug delivery capsule , a carrier of entrapped living cells [37-38], and a food supplement . However, the formation of the alginate membrane has not been investigated as much.
The basic ability of alginate to gel is related to its specific ion-binding characteristics . The variation in gel strength has been analyzed in terms of modes of binding of cation by the various block structures that occur within the alginate molecular chain. Experiments involving equilibrium dialysis of alginate have demonstrated that the selective binding of certain alkaline earth metal ions (e.g., strong and cooperative binding of Ca relative to Mg) increases markedly with increasing content of G-G block in the chains. M-M block and M-G block had almost no selectivity . Regions of homopolymeric blocks of α-L-guluronic acid chelate the alkaline earth metal ions because of the spatial arrangement of the ring and hydroxyl oxygen atoms, and thus create a much stronger interaction . These homopolymeric blocks of α-L-guluronic acid junction zones are constructed mainly of a cross-linked area called an “Egg-box,” where the Ca2+ ions are located as the “Egg” components  (Figure 2). NMR studies of lanthanide complexes of related compounds suggested a possible binding site for Ca2+ ions in a single alginate chain .
3. Membrane Preparation
Many kinds of biopolymer membrane have been utilized and developed in food and biological applications. In general, biopolymer membrane was prepared by casting (e.g., cellulose acetate)  and chitosan ). Spherical gel particles of sodium alginate have often been investigated and applied. However, the formation of alginate membrane has been less investigated. This section provides a general description of the preparation of various alginate membranes.
3.1. Previous Studies on Membrane Preparation
In recent years, alginate membranes have been investigated in diverse ways (e.g., pervaporation, immobilized cell reactor, and ultrafiltration). Previous studies on alginate membrane are listed in Table 2. Teixeira et al. prepared yeast-cell-occupied calcium alginate membrane . Zhang and Franco prepared a calcium alginate membrane for measuring effective diffusivities using the diffusion-cell technique . Grassi et al. determined the drug diffusion coefficient in a calcium alginate membrane . Alginate membrane prepared by low concentration cross-linker needed support matrix (e.g. glass fiber filter) to maintain flat membrane .
Calcium chloride is basically used as a cross-linker in many investigations of calcium alginate membranes. Calcium sulphate and calcium acetate have also been used as cross-linkers for calcium alginate membrane preparation [23, 50-51]. Other cations (Ba2+, Zn2+) have been used as cross-linkers for preparing alginate membrane [49, 51-52]. Barium chloride provided more improved stability than calcium chloride . Zinc acetate can cause denser cross-linking and less selectively than calcium used with sodium alginate .
Sodium alginate membrane cross-linked by glutaraldehyde was applied for acetic acid separation from acetic acid aqueous solution. The membrane was also applied for separating isopropanol from its aqueous solution . Experimental evidence from IR spectroscopy, wide angle X-ray diffractometry, and swelling measurements enabled characterization of the reaction between sodium alginate and glutaraldehyde. The aldehyde groups increased with increasing glutaraldehyde content in the reaction solution .
Kalyani et al. prepared a sodium alginate membrane with phosphoric acid for separating ethanol aqueous solution. Phosphoric acid established a linkage with sodium alginate through ester formation, as confirmed by FTIR .
3.1.2. Hybrid membrane with other polymers
Many efforts have been made to increase the performance of the alginate membrane by blending it with different hydrophilic polymers. Alginate-cellulose using a calcium ion cross-link was investigated in the permeation flux of ethanol aqueous solution for pervaporation . A novel porous composite membrane was prepared using sodium alginate and hydroxyl ethyl cellulose hybrid as an immobilization matrix for humic acid, then cross-linked by glutaraldehyde . Hybrid membranes of sodium alginate and dextrin were prepared by casting followed by cross-linking with glutaraldehyde and used for pervaporation separation of isopropanol aqueous solution . Casting an aqueous solution of alginate with 1,6-hexanediamine or poly (vinyl alcohol) on a hydrolyzed microporous polyacrylonitrile membrane was characterized by pervaporation separation of acetic acid aqueous solution .
The most employed alginate hybrid material was chitosan. Polymer complex membranes made by blending 84% deacetylated chitosan and sodium alginate followed by cross-linking with glutaraldehyde were tested for separating ethanol from ethanol aqueous solution . Sodium alginate and chitosan hybrid membranes were cross-linked with maleic anhydride for separating 1,4-dioxane aqueous solution. Such a membrane has good potential for breaking the aqueous azeotrope 1,4-dioxane .
An alginate-chitosan membrane without a cross-linker could be prepared practically. The structural formation of a chitosan-alginate ion complex was attained between the anion group (-COO-) of sodium alginate and the protonated cation group (-NH3C) of chitosan .
3.2. Preparation of a Flat Alginate Membrane
Our original procedure to prepare calcium alginate membrane by casting was as follows. One gram sodium alginate was dissolved in 100mL water. Sodium alginate samples were provided by Wako Pure Chemical Industries, Ltd. (Osaka, Japan) and KIMICA Corporation (Tokyo, Japan). Calcium chloride (0.05M to 1.0M) was also dissolved in water. Twenty grams of the sodium alginate solution was dispensed on a Petri dish and then completely dried in desiccators at room temperature (298K) for one week. A dried thin film of sodium alginate appeared on the Petri dish. Next, calcium chloride aqueous solution was added directly to the dried thin film of sodium alginate in the Petri dish. A calcium alginate membrane quickly formed in the Petri dish at room temperature. After 20min, the swollen membrane was separated from the Petri dish and then left in the dish for an additional 20min. The membrane was immersed for a total of 40min in the calcium chloride aqueous solution. The formed calcium alginate membrane was soaked in pure water to remove excess calcium chloride aqueous solution, then stored in pure water .
The fundamental gelling mechanism of alginate polymer was the ionic binding reaction between G-G blocks and divalent cations, such as Ca2+. Alginate has high potential of ion exchange. Cross-linking quickly started in the alginate solution. A calcium alginate gel particle was easily obtained by injecting the sodium alginate solution into the calcium aqueous solution . In contrast, the quickly gelling reaction inhibited the preparation of a flat alginate membrane. To overcome rapid gelling, sodium alginate aqueous solution was first dried, and then the cross-linker aqueous solution was directly introduced into the dried alginate surface. As a result, a calcium alginate membrane having a flat surface was successfully prepared. The advanced feature of flat alginate membrane preparation was originally examined by Kashima et al. .
3.3. Evaluation of Components in the Alginate Polymer Chain
As mentioned in chapter 2, components in the alginate polymer chain important factors in investigating the properties of the alginate gel membrane. Two uronic acids, β-D-mannuronic acid (M) and α-L-guluronic acid (G), were constituents of the alginate molecular chain. The homopolymeric blocks of α-L-guluronic (G-G block) in the alginate chain are constructed mainly of a cross-linked zone. Hence, G-G blocks perform a dominant role in the mechanical strength and the mass-transfer characteristics of the calcium alginate membrane .
3.3.1. Qualitative analysis of uronic acid
Mannuronic acid lactone was used as the standard component of uronic acid. As the standard solution, various concentrations of mannuronic acid lactone were dissolved in water. The concentrations were determined by Bitter-Muir’s carbazole sulfuric acid method , and the concentration of the colored solution was measured by optical density at 530nm. The analysis produced good intensity and accuracy of coloration .
3.3.2. Partial hydrolysis of the alginate molecular chain to determine the mass fraction of homopolymeric blocks
The mass of uronic acid in the actual alginate chain was determined by partial hydrolysis combined with Bitter-Muir’s carbazole sulfuric acid method. Partial hydrolysis protocols were employed according to a previous method . Figure 3 illustrates the alginate molecular chain and homopolymeric blocks. The reacted position of partial hydrolysis is marked by an asterisk (*). The sodium alginate chain was separated into three molecular chain blocks (M-G, M-M, and G-G).
Sodium alginate (0.5g) was dissolved in 0.3M HCl (50mL). The resulting solution was heated in an electrical blast-drying chest (373K) for 2h to promote partial hydrolysis. The partial hydrolysis solution was then centrifuged (3000min-1, 15min), and a sample solution of the M-G block was obtained as the supernatant.
The precipitate was mixed with pure water (10mL), and 3M NaOH was added to aid dissolution. The concentration was then adjusted to 1% by the addition of pure water, and NaCl was introduced to achieve 0.1M of sodium alginate. The solution was adjusted to pH 2.9 using 2.5M HCl and then centrifuged (3000min-1, 15min). The sample solution of the M-M block was obtained as the supernatant.
After filtration, the precipitate was mixed with pure water (10mL) and dissolved by adding 3M NaOH, yielding the sample solution of the G-G block. As a result, three sample solutions (M-G, M-M, and G-G) were obtained.
3.3.3. Mass fraction of homopolymeric blocks of α-L-gluronic acid
The mass of the M-G block in the sodium alginate (
The mass fraction of the homopolymeric blocks of α-L-guluronic acid (
3.4. Morphology of the Calcium Alginate Membrane
No stable calcium alginate membrane was obtained using a very low concentration (e.g. less than 0.01M) of CaCl2. It looked like jelly (Figure 4a). A stable, flat, thin membrane was obtained with more than 0.05M CaCl2 solution as a cross-linker (Figure 4b). The membrane became transparent with increasing CaCl2 concentration (Figures 4c, d).
In scanning electron microscopy (SEM) (Miniscope TM-1000, Hitachi, Ltd., Tokyo, Japan), the surface of the membrane appeared to be smooth. No pores were observed on the surface. The higher
Figure 6 presents scanning probe microscope (SPM) (S-image SII Nano Technology, Inc., Tokyo, Japan) images of the membrane surfaces. SPM can determine the morphology of the membrane surface by using the physical force (e.g., atomic force) between the cantilever and the sample membrane. In our case, dynamic force mode/microscopy (DFM) was used for observation. DFM is a measurement technique based on making the cantilever resonant to detect gravitation and repulsive forces against the sample surface. It is a morphology measurement mode for stable observation of relatively sticky, uneven, and soft samples (e.g., biopolymers). The distribution of membrane asperity clearly decayed with increasing
4. Mechanical Properties of the Calcium Alginate Membrane
Investigation of mechanical properties is important for practical application. The following section describes the maximum stress and strain at membrane rupture of the calcium alginate membrane involved with calcium concentration and
4.1. Investigation of Mechanical Strength
The maximum stress and strain at membrane rupture is evaluated by rheometer as a general test of the mechanical properties of the polymer membrane. A swollen membrane sample (10mm wide and 30mm long) was mounted in the rheometer (CR-DX500, Sun Scientific Co., Ltd., Tokyo, Japan) with a crosshead speed of 2mm/s. Maximum stress [N/m2] at membrane rupture was evaluated based on the loading force divided by the cross-sectional area of the membrane. Maximum strain was evaluated as the percentage by which the length increased at membrane rupture divided by the original length of the membrane sample. The relationship between maximum stress and strain with deacetylation degree was investigated as an elastic property of the chitosan membrane using this method .
4.2. Effect of Calcium Concentration
Figure 7 depicts the effect of CaCl2 concentration on the maximum stress at membrane rupture. The maximum stress increased with increasing CaCl2 concentration as a cross-linker. With higher
In contrast, the maximum strain at membrane rupture was remarkably reduced by adding CaCl2 (Figure 8). The cross-linked site became more highly populated with increasing CaCl2 concentration. It was resulted increasing the mechanical strength . Using CaCO3 as a cross-linker, the mechanical properties exhibited profiles similar to those using CaCl2 .
4.3. Effect of Mass Fraction of the Homopolymeric Blocks of α-L-Guluronic Acid (
Mechanical strength and elastic characteristics apparently changed with
4.4. Effect of Cross-Linking Methods
The mechanical properties of calcium alginate membranes prepared from two different CaCl2 treatments were examined by Rhim . One is the direct mixing of CaCl2 into a membrane-making solution (mixing membrane). The other is the immersion of alginate membrane into CaCl2 solution (immersion membrane). With the mixing method, maximum stress and maximum strain at the break of the mixing membrane did not change with increased addition of CaCl2. In contrast, for the immersion membrane, the maximum stress increased and the maximum strain decreased with increased addition of CaCl2. The membrane became rigid. In the immersion method, the mechanical characteristics were strongly influenced by CaCl2 concentration.
4.5. Effect of Relative Humidity
The effect of relative humidity on the mechanical properties of the calcium alginate membrane was examined at relative humidities of 59%, 76%, 85% and 98% at room temperature for 8 days . As relative humidity increased, maximum strain increased and maximum strength decreased.
4.6. Comparison with Other Membranes
Figure 9 indicates the mechanical properties of various polymer membranes. The calcium alginate membrane exhibited high stress and low strain at rupture. It had better mechanical properties than other biopolymer membranes (e.g., chitosan  and cellulose acetate ). The higher
5. Water Content in a Swollen Membrane
The water content of a hydrophilic membrane influences the diffusion phenomena and water permeability. In general, polymer membranes having higher water content have higher water permeability, that has been reported in cellulose acetate membranes . As water occupied mainly the void of the membrane, volumetric water content is often regarded as the void fraction of the membrane structure .
5.1. Evaluation of Water Content
The volumetric water content of the swollen membrane was not measured directly. Instead, it was evaluated from the mass-based water content (
The difference between
The mass-based water content of the swollen membrane (
The volume of water contained in the membrane void was evaluated from
5.2. Effect of Calcium Ion and
F GG on Water Content in Membrane
The dry-based water content of calcium alginate gel beads loading sucrose has been investigated for encapsulation-dehydration of plant germplasm . The dry weight of the beads decreased, and the water content increased with increasing cross-linking time (10 to 30min). The sucrose was diffused to the outer aqueous phase, and then the water penetrated into the gel beads. This is understood as osmotic phenomena surrounding the gel particles. The mass fraction of unfrozen water compared to total water content was also investigated as the thermal property of the gel beads. It increased within 5 to 15min to achieve maximum level (23%), and then declined to minimum level (17%) at 30min.
5.3. Stability of the Swollen Membrane
The stability of the swollen membrane is important to long-life use in practical applications. Rhim focused on the gravimetrical change of the membrane before and after practical use. Stability was evaluated by the dry-base mass of the membrane . Rhim focused on the gravimetrical change of the membrane before and after practical use. Stability was evaluated by the dry-base mass of the membrane . The membrane mass decreased 16% to 20% with increasing soaking temperature (298K to 353K) in aqueous phase. This change was induced by dissolving the membrane matter into aqueous phase. In contrast, the change in membrane mass did not present any significant difference with CaCl2 concentration. The stability of the membrane was affected by soaking temperature but did not depend on CaCl2 concentration.
5.4. Void Fraction of Membrane
The water content of a membrane can be regarded as an indicator of the void fraction (
6. Mass-transfer Characteristics
The diffusivity in calcium alginate “beads” has often been investigated. The effective diffusion coefficient of the alginate “membrane” was originally reported by Kashima et al. . The effective diffusion coefficients are listed in Table 3 and plotted in Figure 11.
6.1. Analysis of Mass-Transfer
The typical procedure to measure mass-transfer flux is as follows. The overall mass-transfer coefficient
Both aqueous phases were sufficiently stirred to create a fully developed turbulent flow. Film mass-transfer resistances
The concentration of the stripping solution was determined by a spectrophotometer (UV Mini 1240, Shimadzu, Kyoto, Japan). The absorbances of the color pigments employed (Methyl orange, Indigo carmine, and Bordeaux S) were measured based on the maximum wavelength (Methyl orange 462nm, Indigo carmine 610nm, and Bordeaux S 520nm). The concentration of urea was determined by absorbance 570nm, according to the urease-indophenol method (Urea NB, Wako Pure Chemical Industries, Ltd., Osaka, Japan). The concentration of glucose was determined by absorbance 505nm, according to the mutarotase-GOD method (Glucose C2, Wako Pure Chemical Industries, Ltd., Osaka, Japan).
6.2. Molecular-Size Screening
Remarkable size-screening capability was obtained between 60Da (Urea) and 604Da (Bordeaux S). The effective diffusion coefficient in the membrane
The remarkable size-screening capability presented in Figure 11 was achieved by prepared 1.0M CaCl2. The membrane composition was expressed as 0.1[mol-Ca2+・g-sodium alginate-1], which is the molar ratio of molar Ca2+ to unit mass of alginate. The molar ratio of Ca2+ to alginate polymer is a dominant parameter of membrane preparation.
The diffusion coefficient in bulk aqueous phase
6.3. Mass-Transfer Characteristics of Urea
The effective diffusion coefficient of urea (60Da) was evaluated mainly for mass-transfer characteristics as a typical small molecule.
6.3.1. Effect of Calcium Ion and
F GG on Mass-Transfer
The effective diffusion coefficient gradually decayed with increasing CaCl2 concentration, due to the progress of cross-linking of molecular frameworks in the membrane (Figure 12). At CaCl2 concentrations above 0.1M, the dependence of the effective diffusion coefficient on the CaCl2 concentration became small . This trend indicates that the molecular frameworks became saturated in this range, and that the effective diffusion coefficient remained almost constant. CaCl2 acted as a cross-linker of molecular frameworks in the alginate molecular chain.
In the higher CaCl2 concentration range, the effect of
6.3.2. Effect of Water Content
There are two special cases of Eq. (8). First, ln (
Figure 13 depicts the ln (
The effective diffusion coefficient in porous materials can be represented by the following diffusion model. This model was applied to analyze mass-transfer in a swollen membrane.
The membrane tortuosity (
Tortuosity of the calcium alginate membrane increased from 16 to 32 with increasing
7. Water Permeation Flux
Water permeation flux is standard technical data for analyzing mass-transfer characteristics of the membrane . Water permeation flux was evaluated based on the gravimetric or volumetric amount of water passing through the membrane. Gravimetric permeate flux (
7.1. Water Permeation Experiment
The typical procedure to measure water permeation flux is as follows. The permeation flux of the calcium alginate membrane was determined from the water mass flux using an ultra-filtration apparatus (UHP-62K, Advantec, Tokyo, Japan). The initial volume of feed solution was constant at 200ml. The operational pressure was adjusted by introducing nitrogen gas. The mass of permeated water was accurately measured by an electric balance and converted to volumetric water flux by recalculation using the density of the permeated water . The experiment was carried out at 298K.
7.2. Effect of Cross-Linker Concentration on Water Permeation Flux
The water permeation flux decreased remarkably with increasing calcium chloride concentration due to progressive cross-linking of the molecular frameworks . High water permeation flux was achieved with low CaCl2 concentration as a cross-linker.
7.3. Dependence on Operational Pressure
Figure 14 illustrates the relationship between volumetric water flux and operational pressure ⊿
7.4. Water Permeation Flux of Other Membranes
Table 4 presents previous investigation results of the water permeation flux of various membranes. The pure water flux of the calcium alginate membrane at ⊿
Ethanol aqueous solution was previously examined in pervaporation using a sodium alginate membrane cross-linked by phosphoric acid. The permeation flux (1.3×10-5 kg m-2 s-1) was less than that of the PTMSP (poly (1-trimethylsilyl-1-propyne)) membrane (5.8×10-5 kg m-2 s-1) [55, 92]. The sodium alginate membrane blended with dextrin was cross-linked with glutaraldehyde to make a stable membrane. It exhibited better water permeation flux of isopropanol aqueous solution than the PVA coating alginate membrane [58, 93]. The water permeation flux was improved with the use of the PVA single component membrane presented by Naidu et al. .
Advanced membrane material from marine biological polymer and sensitive molecular-size recognition for promising separation technology were demonstrated. Stable calcium alginate membrane in swollen state was successfully prepared. The calcium alginate membrane has better mechanical properties than other biopolymer membranes for conventional use. The calcium alginate membrane has a high void fraction (50% to 90%) similar to commercial microfiltration membrane (60% to 90%). Mass transfer characteristics are evidently changed by the mass fraction of α-L-guluronic acid (FGG) and additive CaCl2. Water permeation flux of the calcium alginate membrane is lower than that of other biopolymer membrane (e.g. chitosan, cellulose). In future, the water permeation flux is improved by combination with other polymers (e.g. dextrin). Alginate membrane should be developed as an alternative to artificial polymer membranes.
Sodium alginate samples I-2M and I-2G were kindly provided by KIMICA Corporation (Tokyo, Japan). Professor Hirosi Anzai of Nihon University kindly provided much useful advice for determining the FGG in sodium alginate. Dr. Naoto Arai of Nihon University kindly provided technical advice for SPM observation. Dr. Kei Tao of Nihon University kindly provided technical support of instruments. The authors are grateful to them.
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