TY - CHAP AU - Lev B. Matyushkin AU - Olga A. Aleksandrova AU - Anna O. Drobintseva AU - Igor M. Kvetnoy AU - Yuliya S. Krylova AU - Yaroslav Y. Marchenko AU - Dmitriy S. Mazing AU - Vyacheslav A. Moshnikov AU - Sergey F. Мusikhin AU - Boris P. Nikolaev AU - Victoriya O. Polyakova AU - Maxim A. Shevtsov AU - Ludmila Y. Yakovleva ED - Natalia Grigoryeva Y1 - 2020-09-30 PY - 2020 T1 - Detection of Apoptosis in Cancer Cells Using Heat Shock Protein 70 and p53 Antibody Conjugated Quantum Dot Nanoparticles N2 - Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms. BT - Fluorescence Methods for Investigation of Living Cells and Microorganisms SP - Ch. 10 UR - https://doi.org/10.5772/intechopen.92994 DO - 10.5772/intechopen.92994 SN - 978-1-83968-040-3 PB - IntechOpen CY - Rijeka Y2 - 2024-05-11 ER -