TY - CHAP AU - Cheng-Yang Huang ED - Alessio Innocenti Y1 - 2012-03-07 PY - 2012 T1 - Determination of the Binding Site-Size of the Protein-DNA Complex by Use of the Electrophoretic Mobility Shift Assay N2 - The aim of this book is to provide an overview of the importance of stoichiometry in the biomedical field. It proposes a collection of selected research articles and reviews which provide up-to-date information related to stoichiometry at various levels. The first section deals with host-guest chemistry, focusing on selected calixarenes, cyclodextrins and crown ethers derivatives. In the second and third sections the book presents some issues concerning stoichiometry of metal complexes and lipids and polymers architecture. The fourth section aims to clarify the role of stoichiometry in the determination of protein interactions, while in the fifth section some selected experimental techniques applied to specific systems are introduced. The last section of the book is an attempt at showing some interesting connections between biomedicine and the environment, introducing the concept of biological stoichiometry. On this basis, the present volume would definitely be an ideal source of scientific information to researchers and scientists involved in biomedicine, biochemistry and other areas involving stoichiometry evaluation. BT - Stoichiometry and Research SP - Ch. 10 UR - https://doi.org/10.5772/33676 DO - 10.5772/33676 SN - PB - IntechOpen CY - Rijeka Y2 - 2024-04-26 ER -