The tumor microenvironment is comprised of cancer cells and their surroundings, including various normal cells and non-cellular components, and each tumor tissue has a distinctive microenvironment. Cancer progression is affected by different microenvironmental states, such as the heterogeneity of infiltrating immune cells. Therefore, it is necessary to understand the complex cell-to-cell interactions associated with tumor developmental stages in different tissues. Recent revolution of single-cell RNA sequencing technology can uncover the tumor microenvironment diversity. We have developed a novel strategy of single-cell transcriptome analysis: next generation 1-cell sequencing (Nx1-seq) technology, and it allows for profiling of thousands of single cells from tumor tissue. Our microwell with cell bar-code beads device can detect genes with high sensitivity, and it is easily transported anywhere without any other dedicated devices. Further, the developmental cost is relatively cheaper than other single-cell RNA sequencing methods. In this study, we introduce representative application of the single-cell RNA sequencing technique in gynecological cancers, and we show the result of Nx1-seq application in human endometrioid adenocarcinoma tissue.
- tumor microenvironment
- single-cell transcriptome analysis
Tumor tissues are aggregates of various cell populations, and each single cell or cell population plays an important role for cancer progression and regression. The representative cell populations of the tumor microenvironment are cancer cells, surrounding normal cells, and infiltrated immune cells of all types. Anticancer agents and immune checkpoint blockers, such as programmed death receptor-1 (PD-1) and its ligand, have been widely used in patients, and the curative effect is great. However, for many patients, these treatments are ineffective because the minor cell populations escape the immune system. Therefore, a deeper understanding of the tumor microenvironment immunology will be critical for immunotherapy to become a standard therapy. In addition, it is important to clarify patient and tumor-dependent cell phenotypes by gene expression analysis because the composition and functions of the tumor microenvironment are heterogeneous between cancers and patients.
Previous gene expression measurements have been performed on bulk samples. Conventional bulk-based RNA sequencing or microarrays alone or in combination with flow cytometry can provide a full view of all gene expression, and it is useful to investigate the tumor microenvironment. However, a blended gene expression analysis might mask the minor cell population, which may be the origin of tumor progression. To overcome this problem, RNA sequencing methods that can analyze mRNA expression at the single-cell level from thousands of individual cells are required. The fundamentally necessary approaches of single-cell RNA sequencing are: (1) single-cell isolation with a high survival rate, (2) cell lysis to obtain mRNA, (3) conversion of mRNA into cDNA, (4) specific amplification of cDNA, (5) cDNA fragmentation process, and (6) creation of high-quality sequencing libraries. After single-cell isolation, there are some innovate single-cell transcriptome analysis methods (e.g., CEL-seq , Quartz-seq , Quartz-seq2 , Smart-seq , Drop-seq , iDrop RNA sequencing , Cyto-Seq , automated microwell-based RNA sequencing , and our next generation 1-cell sequencing; Nx1-seq ), and every method uses oligo-dT primers containing cell-specific bar-codes, which tag cDNA from single cells.
Although cell number, tissue volume analyzed, analysis sensitivity, and overall cost for creating libraries are completely different, any methods with an efficient data analysis procedure would be particularly useful to understand cellular heterogeneity and to identify rare cell populations. For example, six prominent single-cell RNA sequencing methods: CEL-seq2, Drop-seq, MARS-seq, SCRB-seq, Smart-seq, and Smart-seq2 have been compared in mouse embryonic stem cells . If single-cell transcriptome analysis were performed in a limited number of cells or small tissue volume, SCREB-seq and MARS-seq will have better sensitivity. Yet, Smart-seq2 may detect the highest number of genes per cell with amplification noise. Drop-seq is a preferable and more cost-effective method for large numbers of cells with low sequencing depth. In terms of the number of reads per cell and genes, we also compared our Nx1-seq and Drop-seq, and it revealed similar sensitivity . Recently, another microwell-based RNA sequencing has been developed , and this is a simple, high-throughput, and low-cost device. The principle of their device is similar to Cyto-Seq and Nx1-seq, but the differences are the beads material and the loading order of single cells and beads to the microwell. They have attempted to construct a “mouse cell atlas” by using over 50 mouse tissues, organs, and cell cultures. One of the reasons they can analyze a large sample amount is the low-cost device without any expensive, exclusive apparatus and kits for capturing mRNA from a single cell. Previously, a detailed description of each method was thoroughly reviewed ; yet, innovate new technologies for single-cell RNA sequencing are still to be developed. We also continue improving our Nx1-seq device progressively.
2. Single-cell transcriptome analysis for cancer tissues
To find new molecular targets for a cancer prognosis prediction method, it requires an understanding of the single-cell level transcriptome heterogeneity in tumor tissues and their microenvironment. Bulk-based RNA sequencing may also contribute to development of new minimally invasive monitoring of circulating tumor cells or cancer gene-transferred macrophages and lymphoid cells. If the targeted cancer antigen and/or cell surface protein were held in small cell populations, the intensity signal of the gene expression would be weak. In this case, single cell transcriptome analysis is a useful tool to identify the small cell population and obtain all of the gene information in this population. In the next chapter, we describe our Nx1-seq methods in detail and show a representative Nx1-seq application in human endometrioid adenocarcinoma tissue. At this time, there are no reports about single-cell transcriptome analysis for endometrioid adenocarcinoma, except our research . Here, we briefly summarize recent applications of single-cell RNA sequencing in one of the major gynecological cancers, breast cancer.
Chung et al. conducted single-cell transcriptome analysis for 11 primary tumors and 2 metastatic lymph nodes from 11 patients, representing 4 breast cancer subtypes . It clearly displayed the carcinoma and tumor-infiltrating immune cells population using the 10–17 μm integrated fluidic circuit mRNA sequencing chip in the C1™ Single-Cell Auto Prep System of Fluidigm®. The C1™ integrate fluidic circuit is an integrated microfluidic system that can automatically isolate individual cells from suspended cells. Subsequently, cell lysis buffer is automatically applied to individual cells to capture mRNA. It takes 5 h to make sequence-ready libraries from isolated cells, and the operation is simple . The authors demonstrated that many T cells with high cytokine and chemokine expression were observed in three triple negative breast cancers (TNBC), and their phenotypes were regulatory T cells (two out of three patients) and another one was exhaustion and cytotoxicity signatures . This result indicates that immune checkpoint blockers may be effective in the patient.
Recently, single-cell transcriptome analysis using 10X Genomics Chromium was reported in breast cancer [15, 16]. Cazet et al. investigated the anti-tumor inhibitor effects in a mouse tumor model, in terms of changes in the gene expression profiles of each cell population . Tumor development and progression were associated with stiffness of the extracellular matrix, and collagen density in the tumor-stromal interface was reduced by small molecule inhibitor of smoothened (SMO) treatment. They also showed that the chemotherapy significantly slowed tumor growth and reduced the frequency of metastatic disease in xenograft models of human TNBC. In another article, an infiltrating T cell population in breast cancer was classified from 123 patients, and it demonstrated the importance of qualitative identification of CD8+ T cell subtypes . CD8+ CD103+ T cells contained features of read tissue-resident memory, including high granzyme B, PD-1, and cytotoxic T lymphocyte (associated) antigen 4 (CTLA-4), rather than CD8+ CD103− T cells, meaning that these are target cells for immune checkpoint brokers.
The above representative reports using single-cell read transcriptome analysis were well analyzed, but we speculate that the cost for creating a sequence library per sample using commercially available device-dependent kits may be expensive. Many samples should be analyzed in a clinical study because the observed microenvironment heterogeneity is patient-, malignant-, or organ-dependent. In addition, if characterization of tumor gene expression profiling was recognized according to the individual’s region or country, it should be performed locally because fresh samples, not frozen ones, are better to analyze for RNA sequencing. From this standpoint, a device with low-cost, in high sensitivity, and easy performance is recommended.
The major component of Nx1-seq (next generation 1-cell sequencing) consists of bar-code beads and a specifically processed microwell. In this chapter, we describe these devices in further detail.
3.1 Bar-code beads
Oligonucleotides on beads have the following sequence: (1) “root array” is used as a priming site for subsequent PCR; (2) “cell bar-code” allocates 12 bp of oligonucleotide to identify cells, and the bar-code has 412 = 16,777,216 various patterns; (3) “UMI” (a unique molecular identifier) has 8 bp of oligonucleotide to eliminate gene duplication bias and improve signal/noise ratio by PCR, meaning that 1-cell bar-code has 1 UMI; and (4) “poly-dT” array consists of 25 bp oligo dT sequences for capturing polyadenylated mRNA. The bar-code beads (“root”-“cell bar-code”-“UMI”-“poly-dT”) were made by following a modified instruction manual for the GS Junior Titanium emulsion PCR Kit (Lib-L) from Roche® Applied Science or synthesized by ChemGenes Corporation (Wilmington, MA, USA) with additional annealing and ligation of the poly-dT array in our laboratory. The detailed method for generating bar-code beads using the emulsion PCR kit is described in our previous report . We could get randomly synthesized various “cell bar-code” inserted bar-code beads, and the beads were washed with Low TE buffer (10 mM Tris-HCl pH 8.0, 0.1 mM EDTA pH 8.0) and stored at −20°C until use.
3.2 Microwell slide
The microwell plate was prepared using polydimethylsiloxane (PDMS) and was cut 2 × 2 × 2 cm using cutting dies (Noda Co. Ltd., Osaka, Japan) which contained 1.3−1.6 × 105 microwells. The size of one microwell was 25 ± 3 μm diameter, 40 ± 8 μm height, 20 ± 9 ρL capacity (column-shape), and the distance between microwells was 5 μm (YODAKA CO., Ltd., Kanagawa, Japan). If the size of the target cell was not between 15 and 25 μm, the diameter and height sizes were easily adjusted. The PDSM microwell plate was placed in an oxygen plasma chamber for hydrophilic processing because PDMS is a hydrophobic material. The microwell plate was quickly set into the Nunc™ Lab-Tek™ Chamber slide system (Thermo Fisher Scientific, Waltham, MA, USA), and bar-code beads were applied to the microwell plate. If the expected number of cells obtained from the tumor tissue was <1 × 105 cells, the PDMS microwell was cut ~1/4 or 1/2 of its size and set into the appropriate Nunc™ Lab-Tek™ Chamber slide system (Figure 1). The PDMS microwell plate was kept at 4°C, meaning that the Nx1-seq device can be stored until use.
3.3 Lysis of cells
After single cell isolation, ~1−2 × 105 cells mixed with 3.7 mL of cold PBS were applied to PDMS microwell plate (2 × 2 × 2 cm) and put the cover without entering a bubble. The microwell plate was put on ice for 10–15 min, which let the cells settle into the microwell by gravity. About 5% of whole microwells were filled with single cells according to Poisson distribution. The solution was removed from the microwell plate, and 1 mL of fresh cold PBS was gently applied. The washing process was repeated by 3–4 times. The reagent composition of 1 mL of cell lysis buffer was; 2 mg of N-Lauroylsarcosine sodium salt, 200 μL of 1 M Tris-HCl pH 7.5, 40 μL of 0.5 M EDTA pH 8.0, 750 μL of deionized water, 50 μL of 1 M dithiothreitol solution. The microwell plate was put on a microscopy, and we found the microwell which contains only cell without bar-code bead, then PBS was removed and 1 mL of cell lysis buffer was gently applied from the corner of the microwell. Most cells were getting to dissolve within 1–3 min, but it kept for 8 min. The cell lysis buffer was removed carefully and washing buffer (200 mM Tris-HCl pH 7.5, 20 mM EDTA, 50 mM DTT, 0.2% N-Lauroylsarcosine sodium salt, 2% Ficoll) was added. Conversion of mRNA into cDNA was done by SuperScript™ II or IV Reverse Transcriptase (Thermo Fisher Scientific).
4. Nx1-seq application to human endometrioid adenocarcinoma tissues
Previously, we reported the application of Nx1-seq to human endometrioid adenocarcinoma (EA) tissues . Here, we summarize the result shortly. EA tissues were removed from the myometrial infiltration side (M-side) and endometrial side (E-side). Myometrial invasion is an independent prognostic parameter of EA, and invasion is correlated with the risk of metastasis to the lymph nodes. Single-cell analysis in each side revealed that EA had six cancer (cluster #0, 1, 2, 3, 5, 6), two macrophage (#4, 8), and one T cell population (#7) (Figure 2A). To analyze the sequencing data, we used Seurat software (
In addition, a cancer stem-like cell population was also higher on the E-side (e.g., the ratio of SOX2+ cells on E-side vs. M-side was 17 vs. 6%, respectively) . These data reveal that cells with high malignant potential (HMP) are present at the same site of cancer tissue (E-side) in EA. To confirm our hypothesis, we focused on the ubiquitin C-terminal hydrolase L1 (UCHL1) gene. Protein ubiquitination or de-ubiquitination regulates cell growth, differentiation, transcription, and tumor prognosis. The function of UCHL1 in neurodegenerative disorders, particularly in Alzheimer’s disease and Parkinson’s disease has been reported, and decreased hydrolase activity and UCHIL1 ligase activity may affect the neurodegeneration [17, 18]. In our EA tissue, the relative intensity of UCHL1 expression was higher on the E-side (Figure 2E). The functional role of UCHL1 in human tumor malignancy is still unresolved, but this gene has been reported to be cancer-related in endometrial cancer patients . Goto et al. demonstrated that activation of UCHL1 via hypoxia inducible factor-1 (HIF-1) is the key regulator for underling mechanism of tumor metastasis, and they expected UCHL1 is as prognostic marker and treatment target for breast and lung cancers .
From the enormous single-cell RNA-sequencing data, the researcher must determine to manage and understand the functional meaning of the cell population. In particular, understanding how the gene is related to overall survival of EA patients in the clinical site is useful. Hence, we used the “cBioPortal For CANCER GENOMICS” website and chose “Uterine Corpus Endometrial Carcinoma (EC) (TCGA, Provisional).” Subsequently, we set “Genomic profiles” as “mRNA Expression,” and chose “mRNA Expression z-Score (microarray),” then input the gene name “UCHL1” (
As shown in Figure 3, there was significant differences about UCHL1 expression between each side, and immunostaining of UCHL1 showed a similar staining pattern in macrophages as well as cancer cells. Also, UCHL1 staining in E-side was relatively higher. These data indicate that microenvironment of tumor tissue might affect the gene properties of immune cells, and gene expression pattern might resemble closely to cancer cells.
The high expression of CXCL3 was not related to the prognosis of EA (p = 0.987) by the CANCER GENOMICS, but CXCL3 high group significantly improved 5-year overall survival by the PROTEIN ATLAS. At this moment, there was only one patient whose expression of CXCL8 was high in the CANCER GENOMICS, but 5-year survival of CXCL8 high or low group in EC patients was 79 or 70% respectively, and there was no significant difference. In contrast, higher expression of NFKBIA significantly decreased survival time by “mRNA Expression z-Sore (microarray)” (p = 0.0246), but not “mRNA Expression z-Score (RNA Seq V2 RSEM)” by the CANCER GENOMICS. In contrast, the 5-year overall survival in the PROTEIN ATLAS was not significant. These results indicate that the researcher must use some database to understand how the target genes are related to the prognosis of cancers. Further studies for other HMP-related genes are ongoing in our laboratory.
Single-cell sequencing is believed to be a powerful tool to answer unknown biological questions, and researchers may have many expectations to find new insights of their hypotheses. Indeed, bulk-based RNA sequencing is averaged across a cell population, but the method to obtain total RNA is relatively simple and easy. Most importantly, we can detect gene expression profiling of the whole tissue. Of course, as mentioned above, the existence of minor cell populations, such as cancer stem-like cells, may not be detected in bulk-based RNA sequencing data. If the researchers knew the biomarkers of targeted cells in small population, the gene can be detected from the bulk-based RNA sequencing data. But it is unknown which cell expresses and how many cells have the targeted gene because of the averaged data by bulk-based RNA sequencing. Thus, it is better to ponder over which method is aimed at the biological question before choosing more difficult and expensive single-cell RNA sequencing.
Current protocols of dispersing single cells in each tissue are not optimized worldwide; therefore, some cells or cell populations may disappear in the course of isolating single cells from tumor tissue. One of the most important procedures for single-cell RNA sequencing is isolation of single cells from tumor tissues. Mechanical and/or enzymatic cell distributed processes followed by fluorescence-activated cell sorting (FACS), magnetic-activated cell sorting (MACS), or density-gradient method are the current standard , but the softness or hardness of tissues differs depending on the tumor. Inappropriate single-cell isolation methods are biased; therefore, more detailed studies are needed to optimize isolation of single cells for each tissue.
Nonetheless, single-cell sequencing is a great tool for detecting heterogeneous subpopulations, cell-to-cell communication, and spatial interactions. Moreover, the many gene expression changes by carcinostatic agents can be monitored. To analyze extensively heterogeneous clinical samples, highly sensitive, low cost, quick, and simple technologies to capture mRNA from a single cell are required. Our newly developed single-cell transcriptome analysis, Nx1-seq, can be a useful tool to understand tumor microenvironments with high sensitivity and low cost. This new approach is a simple method, and it can be used to analyze several hundreds to tens of thousands of cells without specialized equipment. Further, it is easy to change the size of the microwell for larger or smaller cells. Furthermore, microwells equipped with bar-code beads in the Nunc™ Lab-Tek™ Chamber slide system can be stored for several months before use. Nx1-seq is a powerful approach for characterizing cellular diversity under physiological and pathological conditions. The combined analysis of t-SNE by Seurat and detailed gene profiling can discover new tumor biomarkers or new target genes for regression of tumor tissues. We continue to develop better Nx1-seq devices to satisfy requests from researchers. It is about continued learning on a daily basis.
The authors would like to thank Y. Kamide, S. Kurokawa, and H. Sekine for technical assistance with the experiments. We also thank A. Tagata for assistance of accounting work. We greatly appreciate Dr. Y. Takamura for supplying PDMS membrane. Prof. T. Torigoe and Dr. Y. Hirohashi gave us the data of UCHL1 immunohistochemistry and constructive comments, thank you. This work is supported by the Japan Agency for Medical Research and Development (AMED). We would like to thank Editage (
Conflict of interest
The authors have no conflicts of interest directly relevant to the content of this article.