Table 1. Thermodynamic properties for the titration of 0.5 mM gemini surfactant with DNA. aData from Wettig et al., 2010.The binding of cationic alkyammonium surfactants to DNA was studied by Matulis et al., who broke the interaction of the surfactants with DNA down into an electrostatic (ΔelecH) and a hydrophobic contribution (ΔhΦH; Equation 2) (Matulis et al., 2002).
Although the structural and energetic forces involved in DNA condensation have been studied for years, this area has experienced a resurgence of interest in recent years with respect to developing gene therapy protocols to combat a variety of human diseases. Despite an intense effort to study the mechanism(s) of DNA condensation using a variety of techniques such as microscopic, light scattering, fluorescence, and calorimetric techniques the precise details of the energetics of DNA nanoparticle formation and how these complexes assemble is not well understood at present. Isothermal titration calorimetry (ITC) has become an important technique for studying the binding energetics of biological processes, including protein-protein binding, protein-carbohydrate binding, protein-lipid binding, antigen-antibody binding, DNA-protein binding, and the DNA-lipid binding that is at the heart of non-viral transfection vectors. Given the large informational content of thermodynamic data (such as binding constants, stoichiometry, enthalpies of interaction, etc.) it is not surprising that ITC is being used for the elucidation of the binding mechanism of DNA with surfactants or lipids used in non-viral gene delivery vectors. This is described in more detail in this Chapter.
1.1. Principle and experimental set up
One of the fundamental challenges in biophysical chemistry, as well as in all physicochemical events implying a solvent, is to attribute the contribution of different non-covalent interactions (electrostatic in the most general sense, solvation and hydrophocic interactions, hydrogen bonding, Van der Waals interactions) to the free energy change of a given molecule upon its interaction with a binding partner. Another challenge is to investigate how conformational changes in the three dimensional structure of a protein, DNA or RNA can modify the interaction between itself and its binding partner. Finally, the most important challenge is to relate structure (the high resolution X-ray or NMR structure of more and more proteins and nucleotides are available) to the “binding affinity” of these molecules with their binding partners. This means that the “binding affinities” have to be accurately measured for as many as possible systems to get a clear picture of structure-activity relationships, which is particularly important for the design of new drugs or the design of gene vectors for gene therapy. Unlike any other method used to determine thermodynamic parameters associated with bimolecular interactions, calorimetery uses the direct measurement of the heat of interaction (or molar enthalpy ΔH) to probe the extent of binding between the molecules. When substances bind, heat is either generated or absorbed.
Isothermal microcalorimtery is a thermodynamic technique that directly measures the heat released or absorbed during a biomolecular binding event. Measurement of this heat allows accurate determination of binding constants, reaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment. The first modern isothermal microcalorimeter was designed by Calvet (Calvet and Prat, 1963). An ITC instrument consists of two identical cells composed of a highly efficient thermal conducting material (e.g. gold) surrounded by an adiabatic jacket (Figure 1). The jacket is usually cooled by a circulating water bath. Sensitive thermopile circuits detect temperature differences between cells and the jacket, while heaters located on both cells and the jacket are activated when necessary to maintain identical temperatures between all components. In a typical ITC experiment, the macromolecule is placed into the sample cell of the calorimeter and is titrated at constant temperature with the ligand in a syringe. The reference cell contains water or buffer. Prior to injection of the titrant, a constant power (< 1 mW) is applied to the reference cell. This signal directs the feedback circuit to activate the heater located on the sample cell. This represents the baseline signal. During the injection of titrant into the sample cell, heat is taken up or evolved depending on whether macromolecular association reaction is endothermic or exothermic. For an exothermic reaction, the temperature in the sample cell will increase, and the feedback power will be deactivated to maintain equal temperature between the two cells. For endothermic reaction, the reverse will occur.
The heat released (in terms of molar enthalpy ΔH), upon interaction of the titrant is monitored over time. Each peak represents a heat change associated with the injection of a small volume of ligand solution into the ITC reaction cell. As successive amounts of the ligand are titrated into the ITC cell, the quantity of heat absorbed or released is in direct proportion to the amount of binding. When the system reaches saturation, the heat signal diminishes until only heats of dilution are observed. A binding curve is then obtained from a plot of the heats from each injection against the ratio of ligand and binding partner in the cell. A molecular interaction between two ligands can be defined by the following equation which forms the basis for an ITC analysis:
The first part of this equation implies that the change in the Gibbs free energy (Δ
2. Non-viral DNA transfection systems
An emerging trend in the treatment of disease is gene therapy, a broad term that includes any strategy to treat a disease by delivering exogenous gene, gene segments, or oligonucleotides into the cells of patients in order to restore normal functions to the cell. Such therapies are either DNA based (usually in the form of plasmids) or RNA based (usually in the form of small, interfering RNA or siRNA), both requiring some form of delivery system for the oligonucleotide(s) in question.
Two major classifications of delivery systems exist for DNA therapies, viral- and non-viral-based. Viral vectors typically demonstrate the highest gene transfer activity; however, they suffer from serious safety concerns particularly with respect to inflammatory and immune responses in patients. Non-viral vectors are generally comprised of a combination of cationic lipids, neutral lipids and/or cationic polymers, and have a much better safety profile compared to viral systems but suffer from low gene transfer activities. As such a great deal of effort is being expended towards the development of new non-viral vector systems with increased transfection capabilities.
Lipoplexes, complexes of liposomes with DNA, are the most investigated non-viral transfection vectors, and hold great potential application for gene delivery. They have been utilized in approximately 8% of the clinical trials for gene therapy mainly due to their advantages over viral vectors. They show low toxicity, high therapeutic gene capacity, cell-specific targeting capability, and straightforward production, modification, and functionalization procedures. A key problem in the advancement of lipoplex transfection vectors, yet to be efficiently overcome, is the above mentioned inadequacy of transfection efficiency of current cationic lipid systems, attributed to the incomplete understanding of interaction mechanisms involved. While this is driving research efforts toward the design and synthesis of novel amphiphilic cationic compounds, as well as approaches for formulation of liposomal based vectors (Giatrellis et al., 2009), less effort is being directed towards better understanding the fundamental mechanism(s) by which liposome-mediated transfection occurs.
The first reported application of cationic liposomes (or vesicles) as DNA transfection vectors was in 1987, by Felgner et al. They synthesized a cationic lipid, N-[1-(2,3-dioleyloxy)propylJ-N,N,N-trimethylammonium chloride (DOTMA), that forms small unilamellar cationic liposomes, closed bilayer membrane shells of lipid molecules, and that interact with DNA spontaneously via electrostatic attraction. These lipid-DNA complexes maintain an overall positive charge, facilitating uptake by cells again by electrostatic attraction, now between the cationic complexes and the predominantly negatively charged cellular membrane. Complexes are then internalized allowing the transfer of functional DNA into the cell followed by expression. The technique was simple, highly reproducible, and more efficient than some other, commonly used procedures such as use of polycations, calcium phosphate, microinjection, electroporation and protoplast fusion (Felgner et al., 1987).
2.1. Mechanism of DNA – lipid Interactions
Due to electrostatic interactions between negatively charged phosphate groups along the DNA back bone and the cationic head group of the lipid, they compact the DNA plasmid into small particles. This compaction serves two purposes:
1) to more easily transport the DNA across negatively charged cellular membrane(s), both in terms of eliminating electrostatic repulsion between negatively charged DNA and the cell membrane (Barreleiro et al, 2000, Safinya, 2001), and also by simply having a smaller size (relative to an uncomplexed DNA plasmid); and
The importance of the cationic lipid is however not restricted solely to the charge of its head group. (Cherezov et al., 2002). Kreiss et al. believed that both the nature (or structure) of the cationic lipid as well as the DNA backbone exert a strong influence on lipid-DNA packing and complex morphology, with the formation of the lipoplexes resulting from competitive interactions between electrostatic forces of lipid-DNA and elasticity forces driven by the lipid hydrophobic moiety (Zuzzi et al., 2007;Ma et al., 2007;Kreiss et al., 1999).
It has been suggested that lipoplex formation is an endothermic process and the repulsion force between DNA molecules as well as the release of tightly bound counterions from DNA and the lipid mainly controls the formation of cationic lipid/DNA complex (Ma et al., 2007). In the course of complex formation, DNA acts as a polyanion molecule interacting with the cationic headgroups at the surface of liposomes. The neutralization of charges seems to affect both the DNA and the liposome structures. First, DNA might adopt a more compact structure, which is less accessible to intercalating dyes or nucleases. Second, neutralization might induce lipid bilayer mixing and/or aggregation resulting in fusion of liposomes and/or multilamellar structure formation. The fact that different structures are observed in the same lipoplex preparation further complicates the interpretation (Zabner et al., 1995). Apart from the electrostatic interactions between positively charged lipid head groups and the negatively charged DNA phosphate backbone, variations in lipid-lipid and DNA-DNA ionic repulsive forces, attractive lipid-lipid hydrophobic interactions, hydration forces and other structural properties of the liposome and plasmid DNA can result in a wide variety of macromolecular structures that can vary not only with concentration(s) but also as a function of time. Hence, upon mixing of cationic lipid and DNA, the supramolecular organization of the two components changes considerably. Despite these changes in organization, the molecular structures of both components are generally preserved, leading to a so-called multilamellar complex, in which DNA molecules are intercalated between intact lipid bilayers and form a tightly packed grid (Smisterova et al., 2001;Pitard et al., 1999;Cherezov et al., 2002;Radler et al., 1997).
2.2. DNA / lipid complex morphologies
The most commonly complex assemblies include DNA molecules sandwiched within liposomal bilayers, DNA electrostatically adsorbed onto the vesicles outer surface, DNA encapsulated in the aqueous phase of the liposomes, and DNA coated by a monolayer of cationic lipid envelop (Felgner and Ringold, 1989;Giatrellis et al., 2009). Therefore, lipoplexes have a broad range of morphologies from a hypothesized “beads on a string” arrangement proposed initially by Felgner (Felgner and Ringold, 1989) in their seminal paper, to more complicated such as spaghetti and meatballs structure (Sternberg et al., 1994), map-pin structures (characterized by spheroidal heads and tapering pins)(Sternberg et al., 1998)(See Figure 2), multilamellar or inverted hexagonal phase structures confirmed by high-resolution synchrotron small-angle X-ray scattering (SAXS) experiments (Safinya, 2001) and sliding columnar phase (O'Hern, 1998).
These above mentioned morphologies; however, are less common than the lamellar, “sandwich” structure (Koltover et al., 1998;Sternberg et al., 1994). Under lipid excess conditions there is only macroscopic heterogeneity (i.e., lipoplexes may be of different sizes) whereas the microscopic structure of lamellar cationic lipid-DNA “sandwich” is uniform as demonstrated by diffraction techniques (Radler et al., 1997).
2.3. Physiochemical properties, lipoplex structure, and their impact on transfection activity
Structure-activity studies suggested that biophysical properties, such as size, charge, and morphology of the resulting DNA complexes determine transfection efficiency within one class of vector. Several parameters effect transfection activity, such as structural variations of the cationic lipids (length and degree of unsaturation of the alkyl chains), the nature of the groups bound to the quaternary nitrogen, and the counterions. Variations in the lipid to DNA charge ratio and the presence or absence of helper lipids are other important formulation parameters of transfection efficiency (Lobo et al., 2002;Malone et al., 1989). In the majority of reported studies, lipid-DNA complexes function most effectively when a cationic lipid is mixed with a neutral or helper lipid such as DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) or DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) (Scheme 1). DOPE is by far the most commonly used helper lipid in non-viral DNA transfection applications (Ewert et al., 2008;Hui et al., 1996). The inclusion of neutral or zwitterionic lipids (co-lipids), such as cholesterol, DOPE and DOPC as well as PEG lipids, other cationic lipids, and non-lipid compounds like polymers or peptides in the cationic vesicle formulations has been shown to increase the transfection efficiency of the assemblies (at least in case of DOPE) by promoting membrane fusion in different cell lines (Koynova et al., 2007).
2.3.1. Size and zeta potential
In general, transfection efficiency
requires a positive or neutral zeta potential,
is size dependent, e.g., it is higher for smaller size particles, and
requires a vector that is stable in serum.
The size of the DNA plays an important role in biodistribution, cellular internalization and intracellular trafficking (Pathak et al., 2009). The correlation between lipoplex size and DNA transfection has been well studied; however, the most suitable size range (for transfection) still remains to be controversial. Early studies suggested the lipoplex sizes in the range of 400–1400 nm for more efficient transfection in cell culture than smaller (<400 nm) or larger (>1400 nm) aggregates (Kawaura et al., 1998), but later results emphasize a smaller (<200 nm) lipoplexe size range (Zhang et al., 2003). This controversy has been attributed to different size-dependent endocytic pathways involved in uptake of lipoplexes. As the gene transfection by the cationic liposomes was shown to be inhibited by wortmannin, an inhibitor of endocytosis, it is suggested that the vesicles with moderate diameters are useful for gene transfection by endocytosis (Kawaura et al. 1998). Moreover, addition of non-lipid condensing agents such as polyethyleneimine, polylysine, protamine sulfate to the cationic lipid- DNA mixture have been reported to produce small homogeneous lipoplexes, in the range of 100 nm, more suitable to enter narrow capillaries than lipid-DNA alone. Further, the lipoplex particle size also plays a role in DNA release in which for lipoplexes with mean size of ~400–500 nm and lamellar distances of ~5–6 nm (and hence are composed of several tens of layers) extensive intermembrane interactions are required for their disassembly (Koynova, 2009). Zeta potential of lipoplexes is usually measured at different DNA/lipid ratios whereas pure cationic vesicles show a positive zeta potential of ~ 60 mV, and it decreases with addition of DNA and changes to negative at DNA/lipid charge ratio of more than 1 (Koynova et al., 2007). Takeuchi et al showed that transfection efficiency of plasmid DNA (pSV2CAT) into mammalian cultured cells was consistent with the values of zeta potential of cationic liposomes containing a tertiary amino head group(Takeuchi et al., 1996). Therefore, lipoplexes with a small excess of positive zeta charges favours higher transfection efficiency (Elouahabi and Ruysschaert, 2005) and
The role of the cationic lipid molecular structure on transfection activity of resultant lipoplexes has recently been reviewed; with the conclusion that lipids having either saturated alkyl chains with a length of ~14 carbon atoms OR longer, monounsaturated chains having trans-isomerism are generally the most effective for transfection (Koynova and Tenchov, 2009; Koynova et al., 2008). It is important to note that this is only a generalization, and factors such as the stoichiometry of the cationic lipid to DNA, the ionic strength of the formulation, the temperature of the formulation (both during and after formulation), and the incubation time can all have a significant effect on the resulting structure of the lipoplex (Giatrellis et al., 2009).
Association of DNA with cationic lipids in a micellar or liposomal form leads to lamellar organization with DNA molecules sandwiched between lipid bilayers. Although the lamellar phase is the common described structure, as evidenced by small-angle X-ray scattering and electron microscopy, some cationic lipid combined with a hexagonal forming lipid could also result with DNA in an inverted hexagonal structure(Barteau et al., 2008). Despite all the advances in biological and biophysical characterization of cationic lipid-DNA complexes, the relationship of their structural properties to their biological activity is still not well understood. Some earlier studies, especially those carried out on lipoplexes containing DOPE, have proposed the inverted hexagonal structure (HC II) is more efficient in transfection than the lamellar (LC α) phase (See Figure 3) (Koltover et al., 1998;Smisterova et al., 2001). However, more recent studies have shown a general lack of correlation between lipoplex structures and transfection activity. Indeed, high lipofection not only depends on the structure and morphology of the vector assemblies, but also on other factors, (one important example of which is the type of cell being transfected; i.e. primary versus clonal, cancerous versus non-cancerous, etc.). The scope of accounting for all such factors makes it difficult for investigators to put together a comprehensive picture of effects such as variation of both cell types and cationic lipids within one study (Ma et al., 2007).
2.4. Thermodynamics of lipoplex formation
Self-assembly of surfactants into aggregates, such as micelles, vesicles, etc., can be induced by increasing the surfactant concentration to above the critical micelle concentration (CMC) and/or by adjusting the temperature to exceed the critical micelle temperature (CMT). In a typical calorimetry experiment, heats of dilution are recorded. Initially, the dilution of the concentrated surfactant solution (usually well above the CMC) will result in the dis-assembly of micellar aggregates into monomers, and the observed heat of dilution will contain both the heat of solution of the monomer surfactant, and the heat associated with micelle dis-assembling, which is the negative of the heat of micelle formation. As the experiment proceeds, the surfactant concentration in the measurement cell increases to above the CMC, as described in section 1, integration of the measured heats for each injection provides an observed enthalpy of dilution, from which, for a typical surfactant, the enthalpy of micellization (or aggregation), ΔHmic can be obtained from the difference in the pre- and post-micellar enthalpies of dilution. The magnitude and sign (i.e., endo- or exo-thermic) of ΔHmic is governed by the types and magnitude of various contributions (hydrophobic and electrostatic) arising from surfactant-surfactant interactions.
The interaction of cationic aggregates (micelles and/or lipoplexes) with DNA, while being more complex that the aggregation of the surfactants or lipids alone, is governed again by a combination of hydrophobic and electrostatic contributions. The thermodynamics of cationic lipid – DNA lipoplex formation are found to be in most cases, endothermic (Pector et al., 2000;Pozharski and MacDonald, 2002). Since, for any process to be thermodynamically favoured the free energy change must be negative, an endothermic lipoplex formation must be driven by a corresponding increase in entropy (recall equation 1). The interesting question then becomes “what exactly is the source of this entropy gain upon lipoplex formation”? It is widely accepted that the major contribution to this increase in entropy arises from counterion release. Bruinsma first pointed out that lipoplex formation is not simply driven by electrostatic attraction between oppositely charged DNA polymer and lipid membrane, but that in fact the situation is more complex given that both components exist in solution with oppositely charged counterions bound to them that are released upon complex formation (Bruinsma, 1998). Release of these counterions upon complex formation results in an entropy gain that is expected to be, according to Bruinsma, ~1
As an example, Pozharski and MacDonald examined the strength of binding of DNA to the cationic lipid matrix upon lipoplex formation (Pozharski and MacDonald, 2003). In this study, the binding free energy was determined by monitoring lipoplex dissociation under conditions of increasing salt concentration. By using relatively short oligonucleotides, the investigators were able to determine the binding energy per nucleotide. A combination of calorimetry and fluorescence resonance energy transfer (FRET) was used in their work. For FRET studies a rhodamine labeled lipid was brought in proximity with carboxyfluorescein (CF) labeled DNA so that CF emission becomes quenched upon lipoplex formation. The decrease in the signal verified complex formation. A Microcal calorimeter was used to determine the cationic lipid-DNA binding enthalpy for the titration of 0.46 mM Dickerson dodecamer (5′-CGCGAATTCGCG) into 25 μM EDOPC (O-ethyldioleoylphosphocholine, cationic lipid) in HE-S buffer (of 20 mM HEPES, 0.1 mM EDTA, and 150 mM NaCl at pH 7.5). Figure 4 shows the degree of dissociation of complexes, α, plotted against NaCl concentration, and the heat absorbed at various ratios of DNA/ Lipid (D/L).
Averaged over two runs, thermodynamic parameters of the binding were Δ
2.5. Multipoint nature of cationic lipid-DNA interactions
A DNA molecule binds to the lipid membrane at many points and the relatively small free energies of the individual charges sum to a very large binding energy for the whole molecule. The binding of an individual DNA electrostatic charge to the lipid membrane is rather weak (Pozharski and MacDonald, 2003). Hence, the size of DNA does indeed affect binding energy of lipoplex formation. Single lipid molecules cannot dissociate from the complex since they are held in bilayers by strong hydrophobic interactions and thus form a continuous matrix to which DNA can bind. DNA bases are covalently linked to each other and the stiffness of the polymer is such that a molecule only about as short as a dodecamer binds as a single unit. As a result, much more free energy is released upon a binding event and this brings the apparent dissociation constant into the micromolar range at physiological ionic strength. Longer DNA molecules bind more tightly, but their energy of interaction is also limited by their flexibility. The entropy gain upon lipoplex formation is ~1
3. DNA – gemini surfactant systems
3.1. Unsubstituted gemini surfactants
We have recently reported the results of an isothermal titration calorimetric (ITC) study of the interaction of DNA with transfection systems based upon a family of dimeric surfactants, more commonly known as gemini surfactants (Wettig et al., 2010;Yang et al., 2010;Donkuru et al., 2010). Gemini surfactants consist of two traditional surface active groups (i.e two hydrophobic chains and two polar headgroups) linked chemically by a spacer group. The most extensively synthesized and studied family of gemini surfactants are the N,N-bis-α,ω-alkane-diammonium dibromides, having a general structure [CmH2m+1 (CH3)2N+(CH2)sN+(CH3)2 CmH2m+1•2Br-], hereafter referred as m-s-m, where m represents the carbon chain length of the alkyl tails and s the number of carbon atoms in the polymethylene spacer (See Scheme 2A). Compared to conventional surfactants having a single chain and a single head, gemini surfactants posses unique physiochemical properties, including lower critical micelle concentrations (CMCs) and better wetting properties. However, one of the most important advantages, in so far as gene therapy is concerned, is that the structure of gemini surfactants allows for expanded structural diversity through adjustment of the length of hydrophobic chains, the polarity of head groups, the structure of spacer and the counterions. The spacer length plays perhaps one of the most important roles in determining the properties of these surfactants, and therefore likely their interaction(s) with DNA. The effects of variations in the length of a polymethylene spacer group, as well as the effects of incorporation of hydrophilic substitutents have been extensively studied and are the focus of several reviews on gemini surfactants.
It is generally observed that the chemical arrangement of gemini surfactants provide a rather rich array of aggregate morphologies and solution properties that are dependent upon the nature and size of the linking group. Our previous studies indicated that the transfection efficiency and
Microcalorimetry studies used alone and/or along with other techniques can be very helpful in understanding the DNA-gemini interactions in the systems described above. Lipoplexes of gemini compounds, where
The double-peak feature observed in the enthalpogram for the titration of the 12-3-12 surfactant with DNA (Figure 5) is common to all of the symmetric gemini surfactants investigated (so far) by our group. The observed binding interactions have been rationalized in terms of the following steps (Wettig et al., 2010):
An initial interaction between DNA and surfactant micelles; i.e., formation of the “beads on a string” complex – endothermic;
A significant endothermic contribution resulting from the disruption of the micelle-monomer equilibium by continued addition of DNA;
A reorganization of the “beads on a string” complex into an approximately neutral complex which gives rise to an exothermic contribution from both the release of structured water and binding between DNA and surfactant monomer;
Flocculation of the now neutral surfactant – DNA complexes – endothermic;
Precipitation of the complexes (exothermic) followed by no net interaction.
This interaction mechanism is depicted in Scheme 3. The major difference between this mechanism and in the original work published (Scheme 2 in Wang et al., 2007) is that the micelle-monomer equilibrium can no longer be discounted; specifically, the continued addition of DNA to the system has a substantial effect on this equilibrium and results in a significant endothermic contribution to the observed enthalpy. Interestingly a simplification of the system is observed for the pyrenyl surfactants (Scheme 2B), such that a number of intermediate equilibria described above do not seem to occur specifically as a result of the ability for the pyrenyl groups to intercalate between the DNA base pairs.
Other studies have shown that the nature of the counterion significantly affects both micellization and aggregation critical for enhanced trasnfection. The micellization of six cationic gemini surfactants with various counterions, [C12H25(CH3)2N(CH2)6N(CH3)2C12H25] X2, designated as C12C6C12X2, with X = F-, Cl-, Br-, Ac-, NO3 -, and ½ SO4 2 - in aqueous solutions was investigated by microcaloritmetry and conductivity measurements (Jiang et al., 2004). The interaction of these surfactants with DNA in aqueous solutions was also investigated by microcalorimetry. The CMC, CAC (critical aggregation concentration) and the degree of micellar ionization, the saturation concentration of the aggregation, and the associated thermodynamic parameters were detected. Among the counterions examined, SO4 2- was the most effective anion for decreasing the CMC (Koynova et al., 2007). Both aggregation processes were mainly entropy-driven since the values of the entropy changes multiplied by temperature were much larger than the absolute values of the enthalpy changes. The binding of micelles to DNA was strongly dominated by the positive entropy gain on release of the small counterions from the micelles and from DNA. Further, the interaction of all of the surfactants with DNA was dependent on the DNA concentration and may be associated with each micelle interacting with more than one DNA molecule. In a another microcalorimetric study, the interaction of a series of dissymmetric gemini surfactants, (designated as C(m)C(6)C(n)Br2, with constant m+n=24, and m=12, 14, 16, and 18) with DNA in 10 mM NaCl was studied and it was found that the dissymmetry degree has a marked effect on the interaction of the C(m)C(6)C(n)Br2 surfactants with DNA since the CAC and saturation concentration tend to become smaller with increased m/n (Jiang et al., 2005).
3.2. Substituted gemini surfactant systems
A more recent focus of research on gemini surfactants deals with the effect of substitution within the spacer group on the aggregation properties of the surfactant, and the rational design of surfactants with improved transfection activity (Kirby et al., 2003;Wang et al., 2004; Wettig et al., 2002; Wettig et al., 2003; Wettig et al., 2007). Transfection activity is found to depend critically upon the structural elements present (Castro et al., 2004). Recently, pseudoglyceryl gemini lipids bearing an oligooxyethylene (-CH2-CH2-O)m-CH2-CH2-) spacer were found to be superior gene transfecting agents as compared to those bearing polymethylene (-CH2)m-) spacers (Bajaj et al., 2008). In this study, gemini lipids were found to be highly superior in gene transfer abilities as compared to their monomeric lipid and a related commercially available formulation (Bajaj et al., 2008). The increased hydrophilicity of the ethoxylated spacer groups results in an increased water solubility of these compounds, and they preferentially locate at either air-water or micelle-water interface. In some cases, the efficiency of transfection has been correlated to a high extent of DNA condensation, which in turn depends heavily on the gemini spacer structure (Bombelli et al., 2005). An important factor is the ability of the complexes to form polymorphic structures, which are not necessarily hexagonal. Absent from the above, is a thermodynamic characterization of the binding interaction(s) between DNA and gemini surfactants. While the determination of binding properties is complicated by the cooperative nature of the interaction between DNA and gemini surfactants, isothermal titration calorimetry has been used in a few reports.
In order to enhance gene transfection based upon structure-activity relationship, various gemini surfactants (Bombelli et al., 2005) have been designed, synthesized and tested for gene delivery in our laboratory. Previously, we reported the results of a comprehensive study of the aggregation and thermodynamic properties of the 12-4-12 gemini surfactant with and without hydroxyl substitution as well as a series of ethoxylated gemini surfactants. Further, our group is using ITC to extend our work to better understand the thermodynamic properties of the complexation of DNA by the aza-, hydroxyl- and ethoxyl-gemini surfactants, specifically to examine how the binding interactions change as a result of substitution within the spacer group (Scheme 4).
Enthalpograms for the titration of 2.5 mM bp DNA into 0.5 mM gemini surfactant solutions are shown in Figure 6. The interactions between unmodified gemini surfactants (12-3-12, 12-12-12, 18:1-2-18:1, 18:1-3-18:1, 18:1-6-18:1) (Wang et al., 2007a) and pyrenyl-modified gemini surfactants (Wettig et al., 2010) with DNA have been studied previously in this manner, which provides important information on the nature of the interaction(s) between surfactant micelles and DNA for conditions of excess positive charge; i.e., conditions similar to those typically used to prepare complexes for transfection studies.
The same types of interactions observed between unsubstituted gemini surfactants and DNA (described above) will occur for the various substituted gemini surfactants studied in this investigation; however, since the various substitutions are made within the head group region of the surfactant, this should result in noticeable changes in the binding interactions(s) which is indeed the case as seen in Figure 6. The maximum in Δ
steric hindrance between the aza methyl groups and the alkyl tails will not allow the spacer group to fold into the core of the micelle; and
steric considerations also require that at least one of the aza methyl groups be oriented towards the aqueous phase, resulting in a net decrease in the energy associated with the transfer of the spacer from the aqueous to the micellar phase.
The increase in CMC for the N-methyl subtituted series compared to the epoxy series results from increased steric repulsion, particularly with respect to packing at the surface of the micelle, and the interference between the solvation shells around nitrogen and hydrocarbon regions within the spacer (Wettig et al., 2007). A hydrophilic, flexible spacer prompts micelle formation, which leads to a smaller CMC, smaller alpha, larger N, and more negative ΔGmic (Wang et al., 2004). One would expect that the sources of contributions to the enthalpy of binding remain the same within the series. Since the length and composition of the alkyl tails is kept constant, the contribution to Δ
|Surfactant||ΔHinit (kJ mol-1)||P-/N+||ΔH1 (kJ mol-1)||P-/N+||ΔH2 (kJ mol-1)|
The electrostatic component was estimated to be ~1.0 kJ/mol (obtained from the enthalpy of dissolution for ammonium phosphate which allowed for the determination of the hydrophobic contribution from experimental enthalpy measurements. Enthalpy titrations for the alkylammonium surfactant – DNA systems were carried out in the usual (forward) manner of adding concentrated surfactant solution to a solution of DNA, rather than the reverse manner of adding DNA to a concentrated surfactant solution used in our work above. This unfortunately precludes a more detailed analysis and comparison of our results for the substituted gemini surfactants to those for the alkylammonium surfactants (Matulis et al., 2002) as well as the gemini surfactants (Bai et al., 2001;Jiang et al., 2005;Wang et al., 2007; Pozharski and MacDonald, 2002). Forward titrations with the substituted compounds are currently underway to allow for such a comparison.
|Surfactant||ΔHmic (kJ mol-1)||ΔHinit - ΔH1 (kJ mol-1)|
The advantage of the reverse titration method is that it allows for an examination of complex formation under conditions of excess lipid (Kennedy et al., 2000), potentially revealing differences in binding of DNA by lipid or surfactant aggregates (vesicles, micelles, etc.) rather than with monomer lipid or surfactant molecules that would be studied in the forward titration manner. Significant differences in how the resulting complexes assemble have been noted in the comparison of forward and reverse titration methods for the EDOPC – DNA systems introduced in section 2.3 (Kennedy et al., 2000), indicating the potential to control the types, or more specifically structure, of lipid-DNA complexes simply by controlling the order in which the components are added to each other. Such an explanation likely explains the very common observation that transfection efficiencies are highly dependent upon the mixing order of the various components.
Isothermal microcalorimetry plays an important role in elucidating the binding mechanism of DNA with surfactants/ lipids used in non-viral gene therapy. Measurement of heats of interaction allows accurate determination of binding constants, reaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment by employing an appropriate model. Structure-activity studies suggest that biophysical properties, such as size, charge, and morphology of the resulting DNA/lipid complexes determine transfection efficiency within one class of vector. Several parameters effect transfection activity, such as structural variations of the cationic lipids (length and degree of unsaturation of the alkyl chains), the polarity of head groups, the structure of spacer, and the counterions. It is generally observed that the chemical arrangement of gemini surfactants provide a rather rich array of aggregate morphologies and solution properties that are dependent upon the nature and size of the linking group. More recently, ITC has been used to study the interaction of DNA with transfection systems based upon a family of dimeric surfactants or gemini surfactants, which consist of two traditional surface active groups linked chemically by a spacer group. ITC data shows whether the interaction between DNA and gemini surfactants is endothermic or exothermic and the observed enthalpy