Abstract
Weevils are an economically important group of Coleopteran insects of the family Curculionidae. This is the largest insect family in the superfamily Curculionoidea. They may be found almost everywhere and more than 3000 species in near of 500 genera occur in North America. Most of them are plant feeders and others are key pests. These weevils use the snout to feed on plant tissues and notch egg-laying sites on it. Adults drill holes and feed in seeds, fruits and other reproductive parts of the plants. Some of the most notable examples of weevils include Conotrachelus spp. on avocado and guava, Optatus palmaris on anonaceous fruits, Heilipus lauri on avocado, Hypothenemus hampei on coffee berry and others. The presence of some of these species requires establishing measures of restriction when the product is for exportation. Management practices and postharvest treatments are required to ensure that the fruits will be free of larvae. In this chapter the main species of weevils in the most important tropical fruit are included, such as avocado, coffee, guava and anonas fruits. Weevils of economic and quarantine importance are considered.
Keywords
- weevils
- tropical fruit
- management
- importance
1. Introduction
The members of the Curculionidae family are called weevils, since most of them have more or less prolonged head anteriorly as a peak or nose; this term is less appropriate (and rarely used) for Platypodinae and Scolytinae, because there is only little development of the peak in these two subfamilies.
Borers and weevils, including bark and ambrosia, are the members of this family most commonly found. This family shows considerable variation in size and shape. The peak is well developed in most species, with capitate antennae appearing in the middle of the peak. In Scolytinae, Platypodinae and some Cossoninae, there is no peak. The Curculionodae species present a complete metamorphosis (egg, larva, pupa and adult); the family has great economic importance by the number of pest species.
The male external genitalia comes in two types: Ortocera or Gonatocera—in the first one, plates of the aedeagus are known as tecto and the pedon; in the second one, only it remains the pedon; and in some families, there is a clear reduction of the tecto. The antennae are presented in two types—straight or elbowed—in addition some appear to be geniculate [1].
Almost all weevils are phytophagous and among these, there are many important agricultural and forest pests. Almost any part of the plant can be attacked, from the roots to the aerial parts; usually the larvae feed within plant tissues and adults make holes in the fruits, nuts and other parts [2], except for myrmecophilous, saprophagous and predatory species [3].
As mentioned most of them are herbivorous and economically important pest because they affect crops by reducing yields or affect stored products. They can actually cause large losses since the female makes holes somewhere in the plant, either branches or fruits to oviposit. In other cases, females lay eggs on the surface and when the larvae emerge, they pierce the fruit or branches, feeding on plants tissues; in the case of ambrosial weevils, they feed on fungi, which are cultivated within the plant, causing direct and indirect damages.
We considered the main species of weevils in the most important tropical fruits, for example, avocado, coffee, guava and anonas fruits. Weevils of economic and quarantine importance are considered. We talk about topics like biology, importance, damages and suggestions to their control.
2. Big avocado seed weevil, Heilipus lauri Boheman
2.1. Description
The egg of
2.2. Distribution
2.3. Host plants and biology
According to [8] in the State of Mexico,
2.4. Damage
Females drill and lay eggs directly in growing fruits (Figure 2) causing its injury inside; once the larvae emerge, they feed through the pulp to reach the seed where it completes its life cycle. When heavy infestations are recorded and no management is used, infestations of larvae can damage up to 67% of the fruits; in orchards with management of the pest, it has been quantified losses of 3% [9].
3. Small avocado seed weevil, Conotrachelus perseae
3.1. Description
Egg: This is elliptical; the chorion is semitransparent when oviposited and acquires a graying color when the larva is next to emerging; it is less than 1 mm in length [1]. Larva is yellowish white and dark cephalic capsule. Prothorax has a suture open V-shaped and three ventral setae. The mesothorax and metathorax present two dorsal lobes; these segments in the posteroventral region have a dorsal setae. All abdominal segments, except the last one, have three dorsal lobes and three ventral setae. Well-developed larvae reach a length of 6 mm [11, 12]. In pupa, the prothorax has five pairs of lateral setae and three pairs forming two rows on the sides. The mesothorax has two pairs of setae located within a lobe and in the middle part is the other rounded lobe. Abdominal segments are characterized by two pairs of setae in the ventral part of each segment; the eighth and ninth segments present a pair of lateral setae [12]. Adults (Figure 3) are dark brown; prothorax, in dorsal view, is strongly constrained in the apical portion [3, 13–15]. Humeral region of the elytra of
3.2. Distribution
3.3. Host plants and biology
Both weevils have been collected in “Criollo” avocados, Hass and Fuerte [3, 19]. Castañeda Vildozola [17] added
3.4. Damage
Adults damage fruits when they reach 2 cm diameter; fruits are perforated during oviposition. The larvae feed the pulp of the seed. Once concluded the larval stage, the fruits fall to the ground because of the destruction of the seed [12] causing loss of 85% if control measures are not applied.
4. Avocado branch weevil borer, Copturus aguacatae
4.1. Description
The egg is oval in shape, measuring 0.5 mm long and 0.3 mm wide; early oviposited eggs are translucent and then change to white. Larva. A well-developed larva reaches 12 mm in length and is milky white. The head capsule is retracted in the first thoracic segment. The prothorax is ossified in its highest zone and covered with small scales and laterally presents an oval spiracle. In the predorse and postdorse of the mesothorax and metathorax are present two dorsal folds. On the first abdominal segment, there are three dorsal folds and the segments II to VII with four folds. VIII to IX segments have no dorsal folds. All abdominal segments have setae varying in number, size and arrangement. The pupa is exarata and measures 5–8 mm long and 2–2.5 mm wide [9]. They are creamy white. The rostrum reaches the metathoracic coxae. Adults are romboidal shaped, measuring 3.77–5.0 mm long and 2.0–2.5 mm wide [21]. They are black to reddish black with small white scales, red, orange, or black. The females are bigger than the males. They have an almost triangular prosternal ridge between coxae.
4.2. Distribution
The presence of the avocado branch borer has been registered in the states of Morelos, Michoacan, Puebla, State of Mexico, Queretaro, Guerrero, Nayarit and Oaxaca [9, 22, 23].
4.3. Host plants and biology
The avocado is reported as only host [9, 23]. Medina [9] reported that in the state of Morelos,
4.4. Damage
Damage is caused by larvae. The branches affected are those with greater exposure to the sun. Flowers and fruits are not damaged by the larva. The branches are affected from the epidermis to the core; damage is observed on large surface areas covered with lumps of crystallized wise. A consequence of damage, branches in production can be disrupted by the weight of the fruit [23].
5. Control strategies for Heilipus lauri , Conotrachelus perseae ,C. aguacatae and Copturus aguacatae in Mexico
The importance of these species in Mexico is that they are classified as quarantine importance insects; fruit movement to avocado regions free of this pest is not allowed. This restriction is also valid for international market, specifically in the United States where the marketing of Mexican avocados were prevented since 1914 until November 1997 [25, 26].
Mexican Official Standard NOM-066-FITO-2002 has defined areas as free of seed borers and phytosanitary areas under control. The first step to prevent the spread of weevils to free areas of the pest is that the government of Mexico through the SAGARPA considers more rigorous sampling at points of entry and exit of agricultural products and makes strong awareness campaigns for people to be careful with fruit that moves from one place to another [27]. It is important to continue the implementation of studies to learn more about these insects of which is much talk, but little is known.
5.1. Control methods
5.1.1. Cultural control
It is advisable to remove the fruits with signs of damage by seed borers and prune the branches affected by this pest. All these materials should be burned for the control of eggs and immature stages; even though it is a late form of control, it is certain that it will be effective for the next cycle of the crop; less damage will be present, if control is supplemented by other measures. For
5.1.2. Chemical control
In Mexico, some products are approved to be used in avocado orchards; an example is permethrin at doses of 200–300 cc in 100 L of water. The organophosphate insecticides, such as methyl parathion and malathion, are recommended for avocado pest control [9]. In the case of
5.1.3. Legal control
It requires a local legal framework (in the stated affected by the presence of weevils) to attend a plant management program in backyard orchards which are reservoirs of this group of insects. Integrated management campaigns, which according to the NOM-066-FITO-2002 standard is to establish requirements and specifications for the phytosanitary handling and movement of plants and fruits of avocado. This standard indicates that avocado weevils are considered as an obstacle to export fruits; in consequence this is a limitation for exporting Mexican avocados to the US market. This situation remains latent, as producers of avocado in California and Florida are still dissatisfied with market opening arguing great risk of introducing weevils and other insects which may affect avocado orchards in these US states [26].
6. Guava weevil, Conotrachelus dimidiatus
6.1. Description
According to [28] adult presents elytra with discontinuous ridges and is less developed than
6.2. Distribution
In Mexico, it was reported by misidentification of the species
6.3. Host plants and biology
In Mexico, two species of
6.4. Damage
Once adults emerge from the soil, they fly to guava trees to feed; females lay eggs on the middle of developing fruits (Figure 4); after hatching, the larvae penetrate into the fruit to feed. Damaged fruits develop kidney shaped, mature and fall prematurely (Figure 5). In Calvillo, Aguascalientes, Mexico, the infestation of fruits averages 37.4%, which is higher in the lower portion of the tree and during the rainy season, the fruits are more susceptible when they are young, developing two to four cm of polar diameter [33]. The larvae feed the pulp, causing destruction and blackening of this and seeds (Figure 6). If control practices are not done or treatments are performed at inopportune seasons, damages could be extreme [22].
6.5. Control methods
In the orchards, control is based considering the behavior and biology of the insect, according [22, 33] to the presence of adults they have relationship with the rainy season, which are the ones to initiate the damage and infestation of fruit. Based on sampling, using a network under the tree before the start of rainy season, the detection of an adult/tree and observation of fruits with oviposition, applications of chemical insecticides, repellents and entomopathogenic are needed. Local tests to evaluate the effectiveness of these products are recommended; control is complemented with the destruction of damaged fruit that remains attached to the tree. When the fruits are harvested to export to the United States, they are treated by irradiation to eliminate, among other pests, guava weevil. According to [33, 34], the use of chemical attractant traps can be used to sample and detect weevil populations. Biological control is still not a practice used against weevil; we have found parasitoids, predators of larvae and prepupae; however, it is an activity that should be investigated and expand their knowledge in the future. Similarly, the identification of chemical attractants and pheromones as an alternative control should be integrated into weevil control.
7. Coffee berry borer, Hypothenemus hampei
7.1. Description
According to the description [35], the egg is bright white at the beginning to become opaque later. Chorion surface is smooth, measured 0.64 mm long and 0.32 mm in diameter. The larva is white, wormlike and fully developed measuring 1.39 mm in length and has well-developed sclerotic jaws. The pupa is white at the beginning and becomes coffee later; sexual dimorphism exists in relation to size, which is also manifested in adulthood; the female pupa measures 1.18 mm long and 0.88 mm male. The adult is 0.5–0.8 mm in length.
7.2. Distribution
The coffee berry borer (CBB) is native to equatorial Africa and is currently distributed virtually in every country where coffee is grown [36].
7.3. Biology
These insects remain within the fruit much of its life cycle, as egg, larva, pupa and adult stages. The female only leaves to infest new fruit; males remain inside until death making difficult its control [37].
7.4. Damage
In some countries the infestation levels can be up to 90%; in Nayarit, Mexico, it has been recorded up to 70% in orchards with minimum crop management. The female drills close to physiologically mature fruits; inside it lays eggs and, when the larvae emerge, feeds the endosperm of the seed; and it decreases quality and yield.
7.5. Control methods
Due to the wide distribution and importance of the coffee berry borer and the conditions for growing coffee, its control requires a large integrated approach, in which the use of traps with attractants and the application of entomopathogenic microorganisms and parasitoids predominate over the application of insecticide chemicals. The integrated management of the coffee berry borer starts with sampling and determining the economic threshold of action, in Colombia, for example, [38] recommends a maximum fruit infestation of 2% to initiate control activities. The use baited traps for catching and sampling of coffee berry borer is a widespread technique and contributes to both detection and reducing pest significantly. To this respect [39] recommend installation of 22 traps each 10 ha with a mixture of ethanol-methanol 1:3 (v/v) with 1% benzoic acid, as an optimal, effective and inexpensive amount for integrated management of the CBB. The widespread use of
8. Annonaceae weevil, Optatus palmaris
8.1. Description
According to [41], the adult presents strops separated at the apex by a visible keel, middle lobe of the pronotum with a short tubercle on each side and elytra with points of inter-stretch marks forming transverse grooves; the forelimbs are longer in males, with more dilated and bristly shanks (tarsus). The length of prothorax + elytra is 6.5–9.3 mm in males and 6.8–8.6 mm in females. It has a wide body, is rhomboid, has blackening layer, and is covered with fine decumbent scales. The head and face are 7.6–9.0 mm long in males and 7.5–8.2 mm in females and 1.4–1.8 mm wide in male and 1.5–1.7 mm in females, sometimes as long as the prothorax length, inserted at the top of the head in lateral view and slightly curved, reducing its size toward the apex, with lateral grooves in the middle basal; the face (rostrum) of the female is less sculpted than in the male, almost cylindrical, with scores and smaller scale and more limited compared to the male. Middle inserting antennae are 0.53–0.55 in males and 0.52–0.56 mm in females. Antennae with scapum are 0.34–0.38 and 0.33–0.35 times the length of the head (rostrum) or peak in males and females, respectively.
Thorax: Prothorax sometimes is wider than longer. Pronotum is moderately convex; medium lobe is pronounced, subtruncate, slotted dorsally and moderately emarginate and with a short tubercle on each side. The lateral region of prothorax is furrowed near the tip; the lower anterior margin is sub-straight. Mesepimeron is not prominent in the anterior region but visible in dorsal view. Scutellum (1.4–2.3 mm in male) sometimes is as wide as long with the anterior margin sub-straight and acute apex. Elytra (1.8–2.0 in males, females 2.0–2.2) is longer than the prothorax, (1.1 mm male) sometimes as long as broad; subtriangular; humerus is rounded and is present dorsolaterally; pre-apical slang developed; find superficial stretch marks. Description of larval and egg is missing, being a pest of recent detection and increasing their damage, are required to perform morphological studies and evaluate alternative control.
8.2. Distribution
8.3. Host plants and biology
The genus
Adults take refuge in the foliage of their host and feed on vegetative buds, flowers and fruits. They choose fruits close to physiological maturity where they form groups to feed, lay eggs and copulate. It is possible that the adult releases an aggregation pheromone; this may be influenced by volatile compounds of fruits during its developing stage, maybe because in young vegetative buds and flowers, they appear very sporadic and solitary. The adults of
8.4. Damage
8.5. Control
Because of recent detection as Annonaceae pest, considering aspects of its biology and habits, control methods have not been evaluated. It has been observed that it is more frequently damaging in the producing annonaceae areas in Mexico; it is necessary to consider this pest as main insect pest of these crops and evaluate different methods of control and search of natural enemies: parasites, predators and entomopathogenic fungi. Based on their mating and feeding habits recorded by [34, 42], the use of volatile compounds appears to be a good alternative management; however, further studies are required in this regard.
References
- 1.
Leitao B. M. L. Phylogenetic systematic of the tribu Neotropical Optatini Champion, 1907 (Coleoptera, Curculionidae, Baridinae). Instituto Nacional de Pesquisas da Amazonia. Universidade Federal do Amazonas. Programa Integrado de Pos-Graduacao em Biologia Tropical e Recursos Naturais, Manaus, Amazonas. 2007. - 2.
Domínguez J. V. Morphology of small borer seed Conotrachelus perseae Barber. Campus Montecillo, Postgrado en Fitosanidad, Entomología y Acarología, Colegio de Posgraduados, Montecillo, Texcoco, Estado de México. 2006. - 3.
Muñiz V. R. Study morphological of two species of Conotrachelus , that are pests of the avocado (Persea gratísima Gaertn) in Mexico. Rev. Soc. Mex. Hist. Nat. 1970. 31:289–337. - 4.
Castañeda Vildozola A. Bioecology of the big borer seed of the avocado Hilipus lauri Boheman (Coleoptera: Curculionidae) in the region Central de Mexico. Colegio de Posgraduados en Ciencias Agrícolas, Montecillo, Texcoco, Edo. De México. 2008. - 5.
Castañeda Vildozola A., Equihua-Martínez A. and Peña J. E. Avocado weevils of the genus Heilipus , pp. 35–47. In: Peña J. E. (ed.), Potential Invasive Pests of Agricultural Crops. CAB International, London, U.K. 2013. 464 pp. - 6.
García A. P. Hilipus lauri Boheman a borer of the avocado seed in Mexico. Escuela Nacional de Agricultura, Chapingo, México. 1962. - 7.
Palacios-Torres R. E., Castañeda Vildozola A., Sánchez-Pale J. R., Franco Mora O. and Vargas-Rojas L. Interaction of Two Avocado Fruit Insect Borers in the Same Agroecological Area in Mexico. Pan-Pacific Entomol. 2016. 92(2):100–103. - 8.
Salgado-Siclán M. L., Bautista-Martínez N. The big borer seed of the avocado in Ixtapan of the salt, Mexico State. In Memoria. Fundación Salvador Sánchez Colín CICTAMEX, S.C. Coatepec Harinas, Estado de México. 1993. pp. 225–231. - 9.
Medina Q. F. Incidence of the big borer seed of the avocado Heilipus lauri Boheman (Coleoptera: Curculionidae) in Tepoztlan, Morelos. Facultad de Ciencias Agropecuarias. Universidad Autónoma de Morelos. 2005. - 10.
Castañeda Vildozola A., Persea schiedeana (Lauraceae), new host ofHeilipus lauri Boheman (Coleoptera: Curculionidae) in Veracruz, Mexico. Neotrop. Entomol. 2009. 39:914–915. - 11.
Díaz C. F. Biology and control of the small borer seed (Conotrachelus perseae) Barber in the region of Toliman, Queretaro. Escuela Nacional de Agricultura. Chapingo, México. 1972. - 12.
Llanderal De la Peña L. L., Ortega H. A. Biology, habits and identification of the small borer seed of the avocado ( Conotrachelus perseae Barber ) in Ziracuaretiro, Michoacán. Facultad de Agrobiología “Presidente Juárez”. Universidad Michoacana de San Nicolás de Hidalgo, Uruapan, Michoacán. 1990. - 13.
Barber H. S. Avocado Weevils. Proc. Entomol. Soc. Wash. 1919. 21:53–61. - 14.
Barber, H. S. Two New Conotrachelus from Tropical Fruits. (Coleoptera: Curculionidae). Proc. Entomol. Soc. Wash. 1923. 14:181–183. - 15.
Whitehead D. R. Recognition Characters and Distribution Records for Species of Conotrachelus (Coleoptera: Curculionidae ) that Damage Avocado Fruits in Mexico and Central America. Proc. Entomol. Soc. Wash. 1979. 81:105–107. - 16.
González-Herrera A. Arthropods associated with the cultivation of the avocado ( Persea americana Mill.) in Costa Rica. Proceedings of the World Avocado Congress V. Malaga, Spain. 2003. pp. 449–454. - 17.
Castañeda Vildozola Á., Franco-Mora O., Reyes Aleman J. C., Ruiz-Montiel C., Váldez-Carrasco J. and Equihua-Martínez A. New Distribution Records of the Small Avocado Seed Weevil, Conotrachelus perseae Barber (Coleoptera: Curculionidae ), in Mexico and Notes on Its Biology. Coleop. Bull. 2015. 69:267–271. 2015. - 18.
Vázquez A. M., Cruz-López L. and Chamé-Vázquez E. R. First Record of Conotrachelus perseae (Coleoptera: Curculionidae ) in Comitán, Chiapas, Mexico. Fla. Entomol. 2015. 98:1252–1253. - 19.
Salinas C. A. Biology of the small borer seed of the avocado Conotrachelus perseae Barber in Tacambaro, Michoacan. Especialidad en Entomología y Acarología. Instituto de Fitosanidad. Colegio de Posgraduados, Montecillo, Texcoco, Edo. de México. 1999. - 20.
Abreu M. S. Penetration of the Coffee Borer Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae) in Coffee Fruits Different Growing Stages and Selectivity of Insecticides and Fungicides to the Entomopathogen FungiBeauveria bassiana. Universidad Federal de Vicosa. 2002. - 21.
Kissinger, D.G. Description of New Copturus Pest of Avocado from Mexico (Coleoptera: Curculionidae, Zygopinae). Acta Zool. Mex. 1957. 2:1–8. - 22.
Francia R. M. Distribution of seed borers Conotrachelus aguacate Barber andC. perseae Barbe (Coleoptera: Curculionidae) in the municipalities of Tacambaro, Tocumbo, Cotija, Susupuato and Ziracuaretiro, Michoacán. Campus Montecillo, Postgrado en Fitosanidad, Entomología y Acarología, Colegio de Posgraduados, Montecillo, Texcoco, Estado de México. 2008. - 23.
Gasca C. L. Trials with traps adhesive of color and monitoring of Copturus aguacatae Kissinger, in Ziracuaretiro, Michoacan. Instituto de Fitosanidad, Especialidad en Entomología y Acarología. Colegio de Posgraduados. 2000. - 24.
Gudiño J. L. M., García A. M. Biology and habits of the branches and trunks borers of the avocado (avocado Kissinger Copturus) in the region of Uruapan, Michoacán. Facultad de Agrobiología “Presidente Juárez”. Universidad Michoacana de San Nicolás de Hidalgo, Uruapan, Michoacán. 1990. - 25.
Leos-Rodríguez J. A.; Kido-Cruz M. T. and Valdivia-Alcalá R. Impact of the barriers phytosanitary in the trade of avocado between Mexico and the United States of North America. Rev. Chapingo Serie Hort. 2005. 11:99–103. - 26.
Peterson E. V. and Orden D. Avocado Pests and Avocado Trade. Am. J. Agric. Econ. 2008. 90:321–335. - 27.
Espinosa G. N. Validation and simulation statistics of methods for the sampling of Copturus aguacatae Kissinger,Conotrachelus perseae Barber andC. aguacatae Barber; pests of avocado (Persea americana Mill.), in Tingüindin, Michoacan, Mexico. Departamento de Parasitología Agrícola. Universidad Autónoma Chapingo, Chapingo, México. 2007. - 28.
Salas A. M. D., O’Brien C. W. and Romero-Napoles J.. Curculionoidea (Insecta: Coleoptera) from the State of Guanajuato, México. Insecta Mundi. 2001. 15:45–57. - 29.
Sánchez, S. S. New records of phytophagous insects to the State of Tabasco, Mexico. Folia Entomológica Mexicana 2000. 109:113–116. - 30.
Sánchez, S. S. Conotrachelus dimidiatus Champion (Coleoptera: Curculionidae) the weevil of the guava (Psidium guajava L.) in Tabasco, Mexico. Boletin del Museo de Entomología de la Universidad del Valle, Colombia. 2011. 12 (2):17–18. - 31.
O’brien C. W. and Wibmer G. J. Annotated Checklist of the Weevils (Curculionidae sensu lato) of North America, Central America and the West Indies (Coleoptera: Curculionoidea). Memoirs of the American Entomological Institute. 1982. 34:1–382. - 32.
Gonzalez G. E. Control of insect pests. In guava, its cultivation in Mexico. Gonzalez G. E., Padilla R. S., M. L. Reyes, C. M. Perales and V. F. Esquivel (ed.). Libro técnico 1. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Campo Experimental Pabellon. Aguascalientes, México. 2002. 181 p. - 33.
Tafoya F. C., Perales S. E., González G. H. G., Calyecac C. Fruit Damage.Patterns Caused by Ovipositing Females of Conotrachelus dimidiatus (Coleptera: Curculionidae) in Guava Trees. Psyche Res. Artic. 2010. 4 p. - 34.
Maldonado J. E., Hernández L. M. F., Luna G. E., Gómez J. R. A., Flores R. J. C., Santos M. O. Bioecology of Optatus palmaris Pascoe (Coleoptera: Curculionidae) in Annona muricata L Southwest. Entomol. 2014. 39(4):773–781. - 35.
Rubio G. J. D. External morphology of the immature stages of Hypothenemus hampei Ferrari (Coleoptera: Curculionidae). Rev. U. D. C. A. Actualidad y Divulgación Científica. 2009. 12 (2):157–161. - 36.
Le Pelley R. H. Pests of Coffee. Longmans, Green and Co. Ltd., London. 1968. 590 p. - 37.
Vega F. E. Coffee Berry Borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae). 2004. pp: 959–960. - 38.
Pablo Benavides, Carmenza Góngora and Alex Bustillo (2012). IPM Program to Control Coffee Berry BorerHypothenemus hampei, with Emphasis on Highly Pathogenic Mixed Strains of Beauveria bassiana, to Overcome Insecticide Resistance in Colombia, Insecticides - Advances in Integrated Pest Management, Dr. Farzana Perveen (Ed.), ISBN: 978-953-307-780-2, InTech, Available from: http://www.intechopen.com/books/insecticides-advances-in-integrated-pest-management/ipm-program-tocontrol-coffee-berry-borer-hypothenemus-hampei-with-emphasis-on-highly-pathogenic-mix - 39.
Fernandes F. L., Picanco M. C., Fernandes M. E. S., Dangelo R. A. C., Souza F. F. and Guedes R. N. C. A New and Highly Effective Sampling Plan Using Attractant-Baited Traps for the Coffe Berry Borer ( Hypothenemus hampei ). J. Pest. Sci. 2015. 88:289–299. - 40.
Bustillo, P. A. E. The role of biological control in the integrated management of the seed borer of the coffee, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae). Rev. Acad. Colomb. Cienc. 2005. 24(110):55–68. - 41.
Hernández F. L. M., Gómez J. R., Andrés A.J. Importance, pest insect and diseases fungus of the cultivation of the soursoup. Libro Técnico Núm. 1. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Campo Experimental Santiago Ixcuintla, Nayarit. México. 2013. 87 p. - 42.
Corrales M. G. J. Observations on the biology and behavioral food of Optatus sp. (Coleoptera: Curculionidae) inAnnona cherimola Mill. In: Resúmenes de2do . Congreso Centroamericano y del Caribe y 3ero. Costarricense de Entomología. Editorial San José ASENCO. San José, Costa Rica, 17–21 Julio. 1995. p. 6. - 43.
Hernández F. L. M., Valdez J.C., Illescas C. P. R., Maldonado E. J., Orozco M. S. Morphological description of the aedeago of Optatus palmaris Champion (Coleoptera: Curculionidae). Southwest. Entomol. 2012. 37:225–229.