InTech uses cookies to offer you the best online experience. By continuing to use our site, you agree to our Privacy Policy.

Agricultural and Biological Sciences » "Grape and Wine Biotechnology", book edited by Antonio Morata and Iris Loira, ISBN 978-953-51-2693-5, Print ISBN 978-953-51-2692-8, Published: October 19, 2016 under CC BY 3.0 license. © The Author(s).

Chapter 8

Earthworms and Grape Marc: Simultaneous Production of a High-Quality Biofertilizer and Bioactive-Rich Seeds

By Jorge Domínguez, Hugo Martínez‐Cordeiro and Marta Lores
DOI: 10.5772/64751

  1. FAO. FAO STAT 2015 [Internet]. Available from: [Accessed: 2016-04-01].

  2. Alvarez-Casas M, Garcia-Jares C, Llompart M, Lores M. Effect of experimental parameters in the pressurized solvent extraction of polyphenolic compounds from white grape marc. Food Chemistry.2014;157:524–532. DOI: 10.1007/s00217-015-2573-0

  3. Fontana AR, Antoniolli A, Bottini R. Grape pomace as a sustainable source of bioactive compounds: extraction, characterization, and biotechnological applications of phenolics. Journal of Agricultural and Food Chemistry.2013;61:8987–9003. DOI: 10.1021/jf402586f

  4. Negro C, Tommasi L, Miceli A. Phenolic compounds and antioxidant activity from red grape marc extracts. Bioresource Technology. 2003;87:41–44. DOI: 10.1016/S0960-8524(02)00202-X

  5. Kammerer D, Schieber A, Carle R. Characterization and recovery of phenolic compounds from grape pomace: a review. Journal of Applied Botany and Food Quality. 2005;79:189–196.

  6. Inderjit. Plant phenolics in allelopathy. The Botanical Review. 1996;62:186–202.

  7. Barbera AC, Maucieri C, Cavallaro V, Ioppolo A, Spagna G. Effects of spreading olive mill wastewater on soil properties and crops, a review. Agricultural Water Management. 2013;19:43–53. DOI: 10.1016/j.agwat.2012.12.009

  8. Hättenschwiler S, Vitousek PM. The role of polyphenols in terrestrial ecosystem nutrient cycling. Trends in Ecology & Evolution. 2000;15:238–243. DOI: 10.1016/S0169-5347(00)01861-9

  9. Domínguez J, Aira M, Gómez-Brandón M. Vermicomposting: earthworms enhance the work of microbes. In Insam H, Franke-Whittle I, Goberna M, editors. Microbes at Work. Berlin: Springer; 2010. pp. 93–114. DOI: 10.1007/978-3-642-04043-6_5

  10. Aizpurua-Olaizola O, Ormazabal M, Vallejo A, Olivares M, Navarro P, Etxebarria N, Usobiaga A. Optimization of supercritical fluid consecutive extractions of fatty acids and polyphenols from Vitis vinifera grape wastes. Journal of Food Science. 2015;80:101–107. DOI: 10.1111/1750-3841.12715

  11. Makris DP, Boskou G, Andrikopoulos NK. Polyphenolic content and in vitro antioxidant characteristics of wine industry and other agri-food solid waste extracts. Journal of Food Composition and Analysis. 2007;20:125–132. DOI: 10.1016/j.jfca.2006.04.010

  12. Leopoldini M, Russo N, Toscano M. The molecular basis of working mechanism of natural polyphenolic antioxidants. Food Chemistry. 2011;125:288–306. DOI: 10.1016/j.foodchem.2010.08.012

  13. Kim J, Lee KW, Lee HJ. Polyphenols suppress and modulate inflammation: possible roles in health and disease. In: Watson RR, Preedy VR, Zibadi S, editors. Polyphenols in Human Health and Disease. MA, USA: Academic Press; 2014. pp 393–408.

  14. Hertog MGL. Flavonols and flavones in foods and their relation with cancer and coronary heart disease risk [thesis]. Wageningen, The Netherlands: Agricultural University; 1994.

  15. Cordova FM, Watson RR. Food and supplement polyphenol action in cancer recurrence. In: Watson RR, Preedy VR, Zibadi S, editors. Polyphenols in Human Health and Disease. MA, USA: Academic Press; 2014. pp. 191–195. DOI: 10.1016/B978-0-12-398456-2.00016-5

  16. Jean-Gilles D, Li I, Vaidyanathan VG, King R, Cho B, Worthen DR, Chichester CO, Seeram NP. Inhibitory effects of polyphenol punicalagin on type-II collagen degradation in vitro and inflammation in vivo. Chemico-Biological Interactions. 2013;205:90–99. DOI: 10.1016/j.cbi.2013.06.018. Accessed 2014 April 15

  17. Menaa F, Menaa A. Skin photoprotection by polyphenols in animal models and humans. In: Watson RR, Preedy VR, Zibadi S, editors. Polyphenols in Human Health and Disease. MA, USA: Academic Press; 2014. pp. 831–838.

  18. Gollücke AP, Peres RC, Odair A, Ribeiro DA. Polyphenols: a nutraceutical approach against diseases. Recent Patents on Food, Nutrition & Agriculture. 2013;5:214–219. DOI: 10.2174/2212798405666131129153239.

  19. Papandreou MA, Dimakopoulou A, Linardaki ZI, Cordopatis P, Klimis-Zacas D, Margarity M, and others. Effect of a polyphenol-rich wild blueberry extract on cognitive performance of mice, brain antioxidant markers and acetylcholinesterase activity. Behavioural Brain Research. 2009;198:352–358. DOI: 10.1016/j.bbr.2008.11.013

  20. Yilmaz Y, Toledo RT. Major flavonoids in grape seeds and skins: antioxidant capacity of catechin, epicatechin, and gallic acid. Journal of Agricultural and Food Chemistry. 2004;52:255–260. DOI: 10.1021/jf030117h

  21. El Gharras H. Polyphenols: food sources, properties and applications—a review. International Journal of Food Science & Technology. 2009;44:2512–2518. DOI: 10.1111/j.1365-2621.2009.02077.x

  22. Quideau S, Deffieux D, Douat-Casassus C, Pouységu L. Plant Polyphenols: Chemical Properties, Biological Activities, and Synthesis. Angewandte Chemie International Edition. 2011;50:586–621. DOI: 10.1002/anie.201000044

  23. Gómez-Brandón M, Aira M, Lores M, Domínguez J. Changes in microbial community structure and function during vermicomposting of pig slurry. Bioresource Technology. 2011;102:4171–4178. DOI: 10.1016/j.biortech.2010.12.057

  24. Domínguez J. State of the art and new perspectives on vermicomposting research. In: Edwards CA, editor. Earthworm Ecology. Boca Raton, FL: CRC Press; 2004. pp. 401–424.

  25. Domínguez J, Gómez-Brandón M. Vermicomposting: composting with earthworms to recycle organic wastes. In: Kumar S, Bharti A, editors. Management of Organic Waste. Rijeka, Croacia: Intech Open Science; 2012. pp. 29–48. DOI: 10.5772/33874

  26. Domínguez J, Parmelee RW, Edwards CA. Interactions between Eisenia andrei (Oligochaeta) and nematode populations during vermicomposting. Pedobiologia. 2003;47:53–60. DOI: 10.1078/0031-4056-00169

  27. Lores M, Gómez-Brandón M, Pérez-Díaz D, Domínguez J. Using FAME profiles for the characterization of animal wastes and vermicomposts. Soil Biology and Biochemistry. 2006;38:2993–2996. DOI: 10.1016/j.soilbio.2006.05.001

  28. Aira M, Bybee S, Pérez-Losada M, Domínguez J. Feeding on microbiomes: effects of detritivory on the taxonomic and phylogenetic bacterial composition of animal manures. FEMS Microbiology Ecology. 2015;91:117. DOI: 10.1093/femsec/fiv117

  29. Aira M, Olcina J, Pérez-Losada M, Domínguez J. Characterization of the bacterial communities of casts from Eisenia andrei fed with different substrates. Applied Soil Ecology. 2016;98:103–111. DOI: 10.1016/j.apsoil.2015.10.002

  30. Aira M, Domínguez J. Optimizing vermicomposting of animal wastes: effects of dose of manure application on carbon loss and microbial stabilization. Journal of Environmental Management. 2008;88: 1525–1529. DOI: 10.1016/j.jenvman.2007.07.030

  31. Aira M, Sampedro L, Monroy F, Domínguez J. Detritivorous earthworms directly modify the structure, thus altering the functioning of a microdecomposer food web. Soil Biology & Biochemistry. 2008;40:2511–2516. DOI: 10.1016/j.soilbio.2008.06.010

  32. Domínguez J, Edwards CA. Biology and ecology of earthworm species used for vermicomposting. In: Edwards CA, Arancon NQ, Sherman RL, editors. Vermiculture Technology: Earthworms, Organic Waste and Environmental Management. Boca Raton, FL: CRC Press; 2011. pp. 25–37.

  33. Domínguez J, Martínez-Cordeiro H, Álvarez Casas M, Lores M. Vermicomposting grape marc yields high quality organic biofertilizer and bioactive polyphenols. Waste Management & Research. 2014;32:1235–1240. DOI: 10.1177/0734242X14555805

  34. Aira M, Monroy F, Domínguez J. Eiseniafetida (Oligochaeta, Lumbricidae) activates fungal growth, triggering cellulose decomposition during vermicomposting. Microbial Ecology. 2006;52:738–747. DOI: 10.1007/s00248-006-9109-x

  35. Monroy F, Aira M, Domínguez J, Velando A. Seasonal population dynamics of Eiseniafetida (Savigny, 1826) (Oligochaeta, Lumbricidae) in the field. Comptes Rendus Biologies. 2006;329:912–915. DOI: 10.1016/j.crvi.2006.08.001

  36. Nogales R, Cifuentes C, Benítez E. Vermicomposting of winery wastes: a laboratory study. Journal of Environmental Science and Health, Part B. 2005;40:659–673. DOI: 10.1081/PFC-200061595

  37. Romero E, Plaza C, Senesi N, Nogales R, Polo A. Humic acid-like fractions in raw and vermicomposted winery and distillery wastes. Geoderma. 2007;139: 397–406. DOI: 10.1016/j.geoderma.2007.03.009

  38. Gómez-Brandón M, Lazcano C, Lores M, Domínguez J. Papel de las lombrices de tierra en la degradación del bagazo de uva: efectos sobre las características químicas y la microflora en las primeras etapas del proceso. Acta Zoologica Mexicana. 2010;26:397–408. DOI: 0065-1737

  39. Gómez-Brandón M, Lazcano C, Lores M, Domínguez J. Short-term stabilization of grape marc through earthworms. Journal of Hazardous Materials. 2011;187:291–295. DOI: 10.1016/j.jhazmat.2011.01.011

  40. Domínguez J, Gómez-Brandón M. The influence of earthworms on nutrient dynamics during the process of vermicomposting. Waste Management & Research. 2013;31:859–868. DOI: 10.1177/0734242X13497079

  41. Lazcano C., Domínguez J. The use of vermicompost in sustainable agriculture: impact on plant growth and soil fertility. In: Miransari M, editor. Soil Nutrients. New York: Nova Science Publishers; 2011. pp.230–254. DOI: 978-1-61324-785-3

  42. Wu L, Ma LQ, Martinez GA. Comparison of methods for evaluating stability and maturity of biosolids compost. Journal of Environmental Quality. 2000;29:424–429. DOI: 10.2134/jeq2000.00472425002900020008

  43. Hirai MF, Chanyasak V, Kubota M. A standard measurement for maturity. Biocycle. 1983;24:54–56.

  44. He XT, Logan TJ, Traina SJ. Physical and chemical characteristics of selected U.S. Municipal solid waste compost. Journal of Environmental Quality. 1995;24:543–552. DOI: 10.2134/jeq1995.00472425002400030022x

  45. Domínguez J, Lores M, Álvarez Casas M, Martínez-Cordeiro H. Procedimientopara la obtención y aislamiento de un fertilizanteorgánico y de semillas de uva a partir de residuos de uva. 2015. Patent number: ES2533501. 30/11/2015. Universidade de Vigo y Universidade de Santiago de Compostela. Spain.