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Medicine » Infectious Diseases » "Current Topics in Salmonella and Salmonellosis", book edited by Mihai Mares, ISBN 978-953-51-3066-6, Print ISBN 978-953-51-3065-9, Published: April 5, 2017 under CC BY 3.0 license. © The Author(s).

Chapter 14

Application of Ionizing Radiation for Control of Salmonella in Food

By Małgorzata E. Szczawińska
DOI: 10.5772/67408

  1. Leistner L., Gorris L.G.M.: Food preservation by hurdle technology. Trends in Food Science and Technology. 1995;6:41–46.

  2. Farkas J.: Combination of irradiation with mild heat treatment. Food Control. 1990;1:223–229.

  3. Chouliara E., Badeka A., Savvaidis I., Kontaminas MG.: Combined effect of irradiation and modified atmosphere packaging on shelf-life extension of chicken breast meat: microbiological, chemical and sensory changes. European Food Research and Technology. 2008;226:877–888. DOI: 10.1007/s00217-007-0610-3.

  4. Grant I.R., Patterson M.F.: Combined effect of gamma radiation and heating on the destruction of Listeria monocytogenes and Salmonella typhimurium in cook-chill roast beef and gravy. International Journal of Food Microbiology. 1995;27:117–128. DOI: 10.1016/0168-1605(94)00156-Z.

  5. Thayer D.W., Songprasertchai S., Boyd G.: Effects of heat and ionizing radiation on Salmonella typhimurium in mechanically deboned chicken meat. J. Food Prot. 1991;54:718–724.

  6. Sofos J., Flick G., Nychas G., O’Bryan C., Ricke S., Crandall P. Meat, Poultry, and Seafood, p 111-167. In: Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. 4th ed. 2013. DOI: 10.1128/9781555818463.ch6.

  7. Szczawińska M.E., Szczawiński J., Łobacz A.: Effect of temperature on the growth kinetics of Salmonella enteritidis in cooked ham. Bulletin of the Veterinary Institute in Pulawy. 2014;58:47–56. DOI: 10.2478/bvip-2014-0008.

  8. Anonymous: CODEX STAN 106-1983, REV.1-2003 Revised Codex General Standard for Irradiated Foods, 1–10.

  9. Goresline H.E., Ingram M., Macuch P., Mocquot G., Mossell D.A.A., Niven C.F., Thatcher F.S.: Tentative classification of food irradiation processes with microbiological objectives. Nature 1964;204:237–238.

  10. Mulder R.W.A.W.: Salmonella radicidation of poultry carcasses [thesis]. Spelderholt Institute for Poultry Research, Beekbergen, The Netherlands. Report 363. 1982.

  11. European Food Safety Authority: The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2014. European Food Safety Authority, European Centre for Disease Prevention and Control. EFSA Journal 2015;13(12):4329 AMENDED: 4 February 2016.

  12. Anonymous: Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. OJ, L338,22.12.2005, p. 1.

  13. Farkas J.: Radiation processing of dry food ingredients. Radiation Physics and Chemistry. 1985;25:271–280.

  14. Rajkowski K.T., Niebuhr S.E., Dickson J.: Effect of gamma or beta radiation on Salmonella DT 104 in ground pork. Journal of Food Protection. 2006;69:1430–1433.

  15. Miyahara M., Miyahara M.: Effects of gamma ray and electron-beam irradiations on survival of anaerobic and facultatively anaerobic bacteria. Kokuritsu Iyakuhin Shokuhin Eisei Kenkyusho Hokoku. 2002; 120: 75–80.

  16. Webpage IAEA Clearance of Irradiated Foods Database at Internet. Available from

  17. Anonymous: European Commission: Report From The Commission To The European Parliament And The Council On Food And Food Ingredients Treated With Ionising Radiation For The Year 2014. Brussels, 17.12.2015 COM(2015) 665 final. EUR-Lex-52015DC0665.

  18. European Food Safety Authority: Statement summarising the Conclusions and Recommendations from the Opinions on the safety of Irradiation of Food adopted by the BIOHAZ and CEF Panels. EFSA Journal 2011; 9(4): 2107 [155 pp.]. DOI: 10.2903/j.efsa.2011. 2107.

  19. Maxcy R.B., Tiwari N.P.: Irradiation of meats for public health protection. In: Radiation Preservation of Food. IAEA, Vienna, 1973, pp. 491–504.

  20. Dickson J.S., Olson D.G.: Growth rates of Salmonella and Escherichia coli O157:H7 in irradiated beef. Journal of Food Protection 2001;64:1828–1831.

  21. Szczawińska M., Szczawiński J., Szulc M.: The survival of irradiated bacteria during conventional methods of meat preservation. Archiv fur Lebensmittelhygiene 1983;34:53–58.

  22. Szczawińska M.E., Thayer D.W., Phillips J.G.: Fate of unirradiated Salmonella in irradiated mechanically deboned chicken meat. International Journal of Food Microbiology. 1991;14:313–324.

  23. Kim A.Y., Thayer D.W.: Mechanism by which gamma irradiation increases the sensitivity of Salmonella typhimurium ATCC 14028 to heat. Applied and Environmental Microbiology. 1996; 62:1759–1763.

  24. SCF (Scientific Committee on Food). Food - Science and Techniques. Reports of the Scientific Committee for Food (Eighteenth Series). 1986.

  25. Kampelmacher E.H.: Irradiation of food: a new technology for preserving and ensuring the hygiene of foods. Fleischwirtschaft 1984;64:322–327.

  26. Szczawińska M.: Prospects for elimination of salmonellae from poultry by irradiation in Poland. Food Irradiation in the Middle East and Europe. Proceedings of the Final Coordination Meeting of an FAO/IAEA Coordinated Research Programme organized by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture and held in Cadarache, France, 8–12 March 1993. IAEA-TECDOC-754, pp. 77–96, July 1994.

  27. Clavero M.R., Monk J.D., Beuchat L.R., Doyle M.P., Brackett R.E.: Inactivation of Escherichia coli O157:H7, salmonellae, and Campylobacter jejuni in raw ground beef by gamma irradiation. Applied and Environmental Microbiology. 1994;60:2069–2075.

  28. Thayer D.W., Boyd G., Muller W.S., Lipson C.A., Hayne W.C., Baer S.H. Radiation resistance of Salmonella. Journal of Industrial Microbiology. 1990;5:383–390.

  29. Tarkowski J.A., Beumer R.R., Kampelmacher E.H.: Low gamma irradiation of raw meat. International Journal of Food Microbiology. 1984;1:25–31.

  30. Thayer D.W., Boyd G., Fox I. B., Ir., Lakritz L., Hampson I.W.: Variations in radiation sensitivity of foodborne pathogens associated with the suspending meat. I. Food Science. 1995a;60:63–67.

  31. Thayer D.W., Boyd G., Huhtanen C.N.: Effects of ionizing-radiation and anaerobic refrigerated storage on indigenous microflora, Salmonella, and Clostridium botulinum type-A and type-B in vacuum-canned, mechanically deboned chicken meat. Journal of Food Protection. 1995;58:752–757.

  32. Thayer D.W., Boyd G.: Survival of Salmonella typhimurium ATCC 14028 on the surface of chicken legs or in mechanically deboned chicken meat gamma irradiated in air or vacuum at temperatures of -20 to +20 degree. Poultry Science. 1991:70:1026–1033.

  33. Szczawiński J., Szczawińska M., Szulc M.: Combined effects of irradiation and curing salts (NaNO2 and NaCl) upon the behaviour of salmonellae in meat. Archiv fur Lebensmittelhygiene. 1985;36:(3):55–58.

  34. EFSA: SCIENTIFIC REPORT OF EFSA AND ECDC The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013 European Food Safety Authority, European Centre for Disease Prevention and Control European Food Safety Authority (EFSA), Parma, Italy European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden: EFSA Journal. 2015;13(1):3991.

  35. COMMISSION REGULATION (EU) No 1086/2011of 27 October 2011 amending Annex II to Regulation (EC) No 2160/2003 of the European Parliament and of the Council and Annex I to Commission Regulation (EC) No 2073/2005 as regards salmonella in fresh poultry meat.

  36. Kudra L.L., Sebranek J.G., Dickson J.S., Mendonca A.F., Zhang Q., Jackson-Davis A., Prusa K.J.: 2011. Control of Salmonella enterica Typhimurium in chicken breast meat by irradiation combined with modified atmosphere packaging. Journal of Food Protection. 2011 Nov;74(11):1833–1839. DOI: 10.4315/0362-028X.JFP-11-195.

  37. Nassar T.J., al-Mashhadi A.S., Fawal A.K., Shalhat A.F.: Decontamination of chicken carcasses artificially contaminated with Salmonella. Revue Scientifique et Technique. 1997;16:891–897.

  38. Thayer D.W., Boyd G., Fox J.B. Jr., Lakritz L.: Elimination by gamma irradiation of Salmonella spp. and strains of Staphylococcus aureus inoculated in bison, ostrich, alligator, and caiman meat. Journal of Food Protection. 1997;60:756–760.

  39. Commission Regulation (EU) No 365/2010 of 28 April 2010 amending Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs as regards Enterobacteriaceae in pasteurised milk and other pasteurised liquid dairy products and Listeria monocytogenes in food grade salt. OJ L 107, 29 April 2010, pp. 9–11.

  40. Regulation (EC) No 852/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for on the hygiene of foodstuffs. OJ L 139, 30.4.2004.

  41. Regulation (EC) No 853/2005 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for on the hygiene of food of animal origin. OJ L 139, 30.4.2004.

  42. Thayer D.W., Boyd G., Kim A., Fox J.B., Jr., Farrell H.M., Jr.: Fate of gamma-irradiated Listeria monocytogenes during refrigerated storage on raw or cooked turkey breast meat. Journal of Food Protection. 1998;61:979–987.

  43. Responsibilities in relation to producing and handling ready-to-eat (RTE) meats in Queensland. Available from:

  44. Gormley F.J., Little C.L., Grant K.A., de Pinna E., McLauchlin J.: The microbiological safety of ready-to-eat specialty meats from markets and specialty food shops: a UK wide study with a focus on Salmonella and Listeria monocytogenes. Food Microbiology. 2010;27:243–249. Doi: 10.1016/ Epub 2009 Oct 21.

  45. Song H.J., Lee J.H., Song K.B.: Effects of irradiation and fumaric acid treatment on the inactivation of Listeria monocytogenes and Salmonella typhimurium inoculated on sliced ham. Radiation Physics and Chemistry. 2011. Doi:10.1016/j.radphyschem.2011.06.012.

  46. Sommers C.H., Mackay W.J.: Irradiation of Ready-To-Eat Meat Products. In: Institute of Food Technologist Series: Food Irradiation Research and Technology, 2nd ed. Fan X and Sommers CH., editors. 2012. DOI: 10.1002/9781118422557.ch11. This edition first published 2013 © 2013 Blackwell Publishing and the Institute of Food Technologists.

  47. Sommers C.H., Boyd G.: Radiation sensitivity and postirradiation growth of foodborne pathogens on a ready-to-eat frankfurter on a roll product in the presence of modified atmosphere and antimicrobials. Journal of Food Protection. 2006;69:2436–2440.

  48. Cárcel J.A., Benedito J., Cambero M.I., Cabeza M.C., Ordóñez J.A.: Modeling and optimization of the E-beam treatment of chicken steaks and hamburgers, considering food safety, shelf-life, and sensory quality. Food and Bioproducts Processing. 2015;96:133–144. DOI:

  49. IAEA (International Atomic Energy Agency), Vienna, Irradiation to Ensure the Safety and Quality of Prepared Meals. STI/PUB/1365. 2009. ISBN 978-92-0-111108-1.

  50. Kang M., Kim H.-J., Jayasena D.D., Bae S.Y., Yong I.H., Lee M., Jo C.: Effects of combined treatments of electron-beam irradiation and addition of Leek (Allium tuberosum) extract on reduction of pathogens in pork jerky. Foodborne Pathogens and Disease. 2012;9:1083–1087. DOI: 10.1089/fpd.2012.1249.

  51. Bauer N., Cook V., Disney T., Ebel E., Guo C., Johnston J., La Barre D., Lee J., McCoy E., Morrison J., Schlosser W., Williams M.: DRAFT Risk Assessment of the Potential Human Health Effect of Applying Continuous Inspection to Catfish, Prepared by the Risk Assessment Division Office of Public Health Science Food Safety and Inspection Service United States Department of Agriculture. December, 2010.

  52. Hatha AAM and Laksmanaperumalsamy P.: Prevalence of Salmonella in fish and crustaceans from markets in Coimbatore, South India. Food Microbiology.1997;14:111–116.

  53. FDA Guidance for the Industry: Fish and Fishery Products Hazards and Controls Guidance Department Of Health And Human Services Public Health Service Food and Drug Administration Center For Food Safety And Applied Nutrition Office Of Food Safety. Fourth Edition – APRIL 2011.

  54. Huss H.H., Ababouch L., Gram L.: Assessment and management of seafood safety and quality. FAO Fisheries Technical Paper. No. 444. Rome, FAO. 2004.

  55. Reed C.: Import risk analysis: Frozen, skinless and boneless fillet meat of Pangasius spp. fish from Vietnam for human consumption MAF Biosecurity New Zealand, 2008.

  56. Shabarinath S., Kumar H.S., Khushiramani R., Karunasagar I.: Detection and characterization of Salmonella associated with tropical seafood. International Journal of Food Microbiology. 2007;114:227–233. DOI: 10.1016/j.ijfoodmicro.2006.09.012.

  57. FAO: Report of the FAO Expert Workshop on The Application Of Biosecurity Measures To Control Salmonella Contamination In Sustainable Aquaculture. FAO. Mangalore, India, 19–21 January 2010. Fisheries and Aquaculture Report No. 937 FIPM/R937 (En).

  58. Dent F.: Fishery Industry Officer, Fish Products, Trade & Marketing Branch (FIPM): Global Outlook for Shrimp Markets and Demand, 2013, at

  59. Norhana M.N.W., Poole S.E., Deeth H.C., Dykes G.A.: Prevalence, persistence and control of Salmonella and Listeria in shrimp and shrimp products: a review. Food Control. 2010;21:343–361.

  60. Flick G.J., Jr., Granata L.A.: Biological Safety of Fresh and Processed Shellfish. Southern Regional Aquaculture Center (SRAC) SRAC Publication No. 4901, April 2010.

  61. Pinu F.R., Yeasmin S., Bar B.L., Rahman M.M.: Microbiological conditions of frozen shrimp in different food markets of Dhaka City. Food Science and Technology Research. 2007;13:362–365. DOI: 10.3136/fstr.13.362.

  62. Asai Y., Kaneko M., Ohtsuka K., Morita Y., Kaneko S., Noda H., Furukawa I., Takatori K., Hara-Kudo Y.: Salmonella prevalence in seafood imported into Japan. Journal of Food Protection. 2008;71:1460–1464. PMID: 18680947.

  63. Olgunoglu I.A.: Salmonella in Fish and Fishery Products. 2012. In: Salmonella - A Dangerous Foodborne Pathogen, Dr. Dr. Barakat S M Mahmoud (Ed.), ISBN:978-953-307-782-6, Rijaka, Croatia, InTech, Available from:

  64. Brands D.A., Inman A.E., Gerba C.P., Maré C.J., Billington S.J., Saif L.A., Levine J.F., Joens L.A.: Prevalence of Salmonella spp. in Oysters in the United States. Applied and Environmental Microbiology. 2005;71:893–897. DOI: 10.1128/AEM.71.2.893-897.2005. PMCID: PMC546685

  65. Bakr Wafaa M.K., Hazzah Walaa A., Abaza Amani F.: Detection of Salmonella and Vibrio species in some seafood in Alexandria. Journal of American Science. 2011;7:663–668.

  66. Sommers C.H., Rajkowski K.T.: Radiation inactivation of foodborne pathogens on frozen seafood products. Journal of Food Protection. 2011;74:641–644. Doi: 10.4315/0362-028X.JFP-10-419.

  67. Kamat A., Thomas P.: Radiation inactivation of some food-borne pathogens in fish as influenced by fat levels. Journal of Applied Microbiology. 1998;84:478–484.

  68. Jakabi M., Gelli D.S., Torre J.C., Rodas M.A., Franco B.D., Destro M.T., Landgrafi M.: Inactivation by ionizing radiation of Salmonella Enteritidis, Salmonella Infantis, and Vibrio parahaemolyticus in oysters (Crassostrea brasiliana). Journal of Food Protection. 2003;66:1025–9.

  69. Hitoshi I., Harun Or R., Naruemon S., Pitaya A., Pongpen R., Isao I.: Effect of gamma-irradiation on frozen shrimps for decontamination of pathogenic bacteria. Radiation Physics and Chemistry 1993;42:279–282. DOI: 10.1016/0969-806X(93)90249-T.

  70. Sinanoglou V.J., Batrinou A., Konteles S., Sflomos K.: Microbial population, physicochemical quality, and allergenicity of molluscs and shrimp treated with cobalt-60 gamma radiation. Journal of Food Protection. 2007;70:958–966.

  71. Sommers C.H., Rajkowski K.T., Sheen S., Samer C., Bender E.: The effect of cryogenic freezing followed by gamma radiation on the survival of Salmonella spp. on frozen shrimp. Journal of Food Processing & Technology2011;S8.

  72. Nerkar D.P., Bandekar J.R.: Elimination of Salmonella from frozen shrimp by gamma radiation. Journal of Food Safety, 1990;10(3):175–180.

  73. Luo X., Cui S., Li Y., Jiang T.: A Study On The Use Of Irradiation In Combination With Vacuum Packaging To Produce Non-Sterile Shelf-Stable Food, Semi-Dried Meat, Seafood And Vegetables. In: Radiation Processing For Safe, Shelf-Stable And Ready-To-Eat Food. Proceedings of a final Research Co-ordination Meeting held in Montreal, Canada, 10–14 July 2000. IAEA, VIENNA, 2003 IAEA-TECDOC-1337 ISBN 92–0–100703–5 ISSN 1011–4289, p.194-198.

  74. Tambunan P.R.: Elimination of Salmonella in frog legs by irradiation. Final Report to the International Atomic Energy Agency on Contract No 2711/R1/RB. Central Research Institute for Fisheries, Indonesia. 1984.

  75. Federal Register: Irradiation in the Production, Processing and Handling of Food, A Rule by the Food and Drug Administration on 11/30/2012 Fed Regist. 2012 Nov 30;77(231):71312–71316.

  76. Farkas J.: Irradiation of poultry meat. In: Food Safety Control in the Poultry Industry. G. Mead. Cambridge, 2005:433–452.