Reports showing the implication of TRPM2 in immune reactivities using
The transient receptor potential (TRP) family includes cation-permeable ion channels activated by diverse stimuli ranging from chemical compounds, mechanical force and temperature. TRPA1, TRPV1, TRPC5, TRPM2, and TRPM7 are reported to be activated by reactive oxygen species (ROS). The sensitivity of TRPs to ROS is in part associated with pathogenesis caused by ROS and physiological adaptations to redox signals. The present review focuses on the well-defined ROS-sensitive TRP channels, TRPA1 and TRPM2, and summarizes recent reports regarding their activation mechanism by ROS and their relevance to pathological conditions and physiological functions in which ROS are involved.
- transient receptor potential (TRP) channel
- calcium signal
- body temperature
Reactive oxygen species (ROS) are oxygen-derived molecules with high reactivity that are generated in diverse pathways, including by proteins of the mitochondrial respiratory chain, NADPH oxidase (NOX), dual oxidase (Duox) and cyclooxygenase (COX). Although ROS have been considered as deleterious by-products, they are generated in response to many physiological stimuli such as cytokines, hormones and mechanical force to act as signaling molecules in parallel with reactive nitrogen species (RNS), including nitrogen oxide (NO) . These cellular “redox” signals are considered to play important roles in a wide range of physiological phenomena [1–3]. In the immune system in particular, H2O2 produced by NOX is important for removal of microorganisms and defects in redox signaling are associated with persistent infections . In contrast, unregulated ROS production in pathological conditions leads to chronic inflammation and tissue damage.
The transient receptor potential (TRP) family is a superfamily of nonselective cation channels that are homologous to the founding TRP member in
TRPA1 is the exclusive member of the TRPA subfamily and was named for its characteristic N-terminal long 18 ankyrin repeat domain (ARD), a motif that is thought to mediate protein–protein interactions . TRPA1 is abundantly expressed in nociceptive primary afferent sensory neurons that detect nociceptive stimuli. TRPA1 is expressed in sensory neurons of dorsal root ganglia (DRG), trigeminal ganglia (TG), and nodose ganglia, as well as many other organs and tissues, including the brain, heart, small intestine, lung, skeletal muscle, and pancreas . Because TRPA1 has broad sensitivity to reactive compounds, it can detect a wide range of hazardous compounds (see below). TRPA1 function is modulated by endogenous intracellular factors such as membrane lipids and Ca2+, which has bimodal effects on TRPA1 function. EF-hand-like domains in the N-terminus between the 11th and 12th ankyrin repeat of TRPA1 are reported to form a Ca2+-binding site responsible for Ca2+-dependent mTRPA1 activation [10, 11]. TRPA1 desensitization can also depend on Ca2+ .
TRPM2 is a member of the TRPM subfamily that functions as a nonselective cation channel. TRPM2 is known as a “channezyme,” that is, an ion channel that possesses an enzymatic region, based on its C-terminal Nudix domain that is homologous to the NUDT9 adenosine diphosphoribose (ADPR) pyrophosphatase, an enzyme that converts ADPR to adenosine monophosphate and ribose 5-phosphate . However, the TRPM2 Nudix domain lacks ADPR hydrolase activity and its binding to ADPR is sufficient for channel gating [14, 15]. Adenosine diphosphoribose (ADPR), cyclic ADPR and pyridine dinucleotides, including nicotinamide-adenine-dinucleotide (NAD), nicotinic acid-adenine dinucleotide (NAAD) and NAAD-2′-phosphate (NAADP), have been reported as endogenous agonists for TRPM2. However, TRPM2 activation by pyridine dinucleotides was later shown to have been caused by ADPR contamination, and thereby, pyridine dinucleotides are likely not TRPM2 agonists . TRPM2 is also activated by reactive oxygen species  and is therefore considered to be redox-sensitive. TRPM2 is regarded as a “metabolic sensor” because its activity is regulated by cellular and systemic metabolic states such as redox signaling, glucose levels and body temperature [18–20]. As with TRPA1, Ca2+ plays crucial roles in TRPM2 activation , and Ca2+ binding to the intracellular pore cavity is necessary for channel activation . Meanwhile, intracellular H+ ions reportedly inhibit TRPM2 function .
TRPM2 is expressed in the plasma membrane of many cell types such as neurons and microglial cells in the brain, vascular endothelial cells, pancreatic β-cells and immunocytes, as well as tissues in the spleen and liver . Several reports showed that TRPM2 also functions in the lysosomal membrane [25, 26].
Despite fluctuating environmental temperatures, endothermic species such as mammals and birds can maintain a constant body temperature through the activity of peripheral thermal sensors in sensory and autonomic neurons and by thermosensitive structures within the preoptic area (POA), anterior hypothalamus, brain stem, spinal cord and possibly other places that regulate body temperatures through autonomic and behavioral mechanisms . TRPM2 was recently shown to be expressed in warmth-sensitive neurons of the POA and in peripheral sensory and autonomic neurons, suggesting that TRPM2 is involved in regulating body temperature [28, 29].
2. Activation mechanism of TRPA1 by reactive oxygen/nitrogen species and diverse chemical compounds
TRPA1 is expressed in primary sensory neurons and mediates noxious pain sensations evoked by a wide range of reactive compounds. Electrophilic TRPA1 activators have environmental, dietary and endogenous origins and include acrolein (air pollutant), allyl isothiocyanate (in mustard), allicin and diallyl disulfide (in garlic), cinnamaldehyde (in cinnamon) and the proalgesic lipid peroxidation products 4-hydroxynonenal (4-HNE) and 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) (Figure 1A) [30–33]. These compounds are collectively referred to as reactive carbonyl species (RCS). Many have highly reactive electrophilic carbon moieties such as an αβ-unsaturated carbonyl group. These moieties react with cysteine thiol groups in TRPA1, including those located between the N-terminal ARD and S1 (Cys621, Cys641, Cys665) of human TRPA1, which results in covalent modification by S-alkylation through a Michael addition that in turn activates TRPA1 (Figure 1B) . In mouse, TRPA1, Cys415 and Cys422 in ARD and Cys622 between ARD and S1 are required for activation by reactive compounds . In addition to cysteine residues, lysine (Lys708 in human TRPA1) is also reportedly involved in TRPA1 activation by reactive electrophilic species, although the contribution of this amino acid seems to be limited to the action of AITC .
TRPA1 is also activated by reactive oxygen/nitrogen species (ROS/RNS) and thus is regarded to be “redox-sensitive”. TRPA1 is activated by many kinds of ROS and RNS, including hydrogen peroxide (H2O2), hydroxyl radical (OH·), hypochlorite (OCl−), nitric oxide (NO) and peroxynitrite (ONOO−) [32, 34, 35] (Figure 1A). Oxidative stress endogenously generates lipid peroxidation products such as 4-NHE and 15d-PGJ2, described above as RCS. However, ROS can activate TRPA1 independently of these peroxidation products, as evidenced by the finding that H2O2-induced TRPA1 activation is reversed by the application of dithiothreitol (DTT), which reverses cysteine disulfide formation, nitrosylation and cysteine oxidation, but not Michael addition, without affecting the action of 4-NHE and 15d-PGJ2 [32, 36]. This result suggests that ROS induce cysteine oxidation and disulfide formation between proximal cysteine residues in TRPA1.
TRPA1 activation by RNS is considered to be mediated by S-nitrosylation of cysteine residues, since TRPA1 activation by the NO donor SNAP was reversed by the application of DTT as described above . Functional characterization of site-directed cysteine mutants of mouse TRPA1 demonstrated that modification of several cysteine residues (Cys621, Cys641, Cys665) involved in RCS activation mediates the action of ROS and RNS [34, 35]. However, mutation of cysteine and lysine residues could also affect channel activation by nonelectrophilic agonists, which would complicate the identification of amino acids responsible for covalent modification of TRPA1 . TRPA1 activation by these reactive compounds leads to pain sensation that would diminish the likelihood of deleterious damages arising from adducts formed by these compounds and DNA or proteins that are associated with carcinogenesis and toxicity .
3. Activation mechanism of TRPM2 by redox signaling
TRPM2 channels are activated by H2O2 , and this activation could be enhanced by pretreatment with Fe2+ to promote formation of hydroxyl radicals generated by the Fenton reaction that are also thought to have an important role in TRPM2 activation . Indirect TRPM2 activation through the production of intracellular ADPR and direct action independent of ADPR production have also been reported (Figure 2A). One ADPR production pathway involves nuclear poly(ADPR) polymerases (PARPs) and poly(ADPR) glycohydrolases (PARGs). PARP is activated by DNA damage upon oxidative stress and has DNA repair functions . Meanwhile, PARG hydrolyzes poly-ADPR chains to generate free ADPR. The finding that treatment of cells with a PARP inhibitor decreased H2O2-mediated TRPM2 activation supports a role for ADPR in activation of this channel . Another possible source of ADPR is mitochondria, where oxidative stress could induce the production of free ADPR . Indeed, reduced mitochondrial ADPR concentrations suppressed H2O2-mediated TRPM2 currents . ADPR can also be produced by plasma membrane/intracellular organelle CD38, which has implications for immunocyte function [43, 44]. However, the mechanism by which extracellular ADPR generated by the CD38 ecto-enzyme enters the cells is unclear . In contrast, an ADPR-independent TRPM2 activation pathway may also exist based on the finding that a TRPM2 variant lacking the Nudix domain that contains the ADPR-binding domain still responded to H2O2 . In addition, our recent results showed that H2O2 clearly enhanced heat-evoked responses of TRPM2 in inside-out single channel recordings in which intracellular components were completely absent (Figure 2B) . This action was accompanied by a reduction in the temperature threshold for TRPM2 heat activation (Figure 2C) and was mimicked by an oxidant that reacts with methionine residues. Taken together, these results suggest that ADPR-independent actions could also be involved in TRPM2 activation; however, because H2O2-evoked reduction in the temperature threshold for TRPM2 activation was affected by a PARP inhibitor, there could be both direct and indirect actions in H2O2-mediated effects.
4. Pathological and physiological relevance of redox signal-regulated TRPA1 activation
TRPA1 is abundantly expressed in a portion of primary sensory neurons and mediates noxious pain sensation evoked by ROS to avoid deleterious DNA damage. In an inflammatory milieu, phagocytes release large amounts of ROS that promote the production of endogenous lipid compounds, including the RCS 4-HNE and 15d-PGJ2. These compounds activate TRPA1 to cause inflammatory pain [36, 47].
TRPA1 is readily modified by electrophilic compounds. An analysis of the redox potentials of reactive disulfides revealed that TRPA1 has the lowest redox potential threshold among TRPA1, TRPV1, TRPV2, TRPV3, TRPV4, and TRPC5, indicating its high sensitivity to electrophilic compounds . Thus, TRPA1 could act as a hyperoxia sensor in vagal and sensory nerves (Figure 3). Heterologously expressed TRPA1 was activated through covalent modification of cysteines at high oxygen conditions in excess of atmospheric oxygen concentration (20% O2) (hyperoxia), and the activation was diminished by point mutation of TRPA1 cysteine residues (Cys633Ser and Cys856Ser) (Figure 1B). In addition, hypoxic conditions also activated TRPA1, which could be suppressed by overexpression of recombinant prolyl hydroxylase (PHD). The oxygen-sensitive PHD catalyzes proline hydroxylation of TRPA1 under normoxic conditions that in turn inhibits channel function. A PHD inhibitor activated TRPA1 even under normoxic conditions, suggesting the involvement of proline hydroxylation in TRPA1 activation. Under hypoxic conditions, basal enzymatic activity of PHD is inhibited and TRPA1 is activated upon relief of proline hydroxylation-mediated inhibition. A proline residue located in the TRPA1 N-terminus (Pro394) is suggested to be involved in proline hydroxylation-mediated TRPA1 inhibition (Figure 1B). Indeed, vagal nerves isolated from wild type, but not TRPA1-knockout (KO) mice, showed [Ca2+]
TRPA1 is also reported to play a physiological role in artery vasodilation, although its activity varies among different types of vascular beds. In cerebral arteries, TRPA1 expressed in the endothelium mediates “endothelium-dependent” vasodilation. ROS such as superoxide anions (O2−) and hydrogen peroxide (H2O2) are known to cause vasodilation, leading to increases in cerebral microcirculation . TRPA1 mRNA expression was observed in the endothelium of cerebral arteries, but not in peripheral vascular beds in renal, coronary and mesenteric arteries . TRPA1 protein in the endothelium of cerebral arteries preferentially colocalizes with NOX2, a ROS-generating enzyme, in fenestration of internal elastic lamina where plasma membranes of endothelium and smooth muscle cell are in close contact. ROS generated by NADPH-induced NOX activity led to cerebral artery vasodilation following TRPA1 activation. This vasodilation could be abolished in a variety of ways, including by a NOX inhibitor, catalase-mediated degradation of H2O2, deferoxamine, which inhibits the Fenton reaction that generates hydroxyl radical (OH−), the TRPA1 inhibitor HC-030031 and TRAM34, a blocker of the intermediate conductance Ca2+-sensitive K channel (IK). Moreover, vasodilation could be mimicked by application of 4-hydroxy-nonenal (4-HNE), a product of lipid peroxidation. These results suggest that ROS-derived lipid peroxidation products activate TRPA1, leading to cytosolic Ca2+ elevation, which in turn activates intermediate conductance Ca2+-sensitive K channels (IK) and membrane hyperpolarization in the endothelium. This change in membrane potential is propagated through gap junctions to smooth muscle cells to promote additional vasodilation. On the other hand, in peripheral arteries, TRPA1 expressed in primary sensory neurons is reportedly involved in “neurogenic” vasodilation. TRPA1 expressed in sensory neurons likely mediates vasodilation in peripheral arteries because TRPA1 is not expressed in the endothelium of peripheral arteries . Topical application of cinnamaldehyde, a TRPA1 agonist, onto mouse ears caused vasodilation in wild-type mice, but not in TRPA1KO mice. TRPA1 agonist-induced vasodilation could be attenuated by a CGRP antagonist, a nonselective NOS or a neuronal NOS (nNOS) inhibitor, suggesting the possible involvement of CGRP and NO release from sensory neurons. In addition, TRPA1 agonist-induced vasodilation is mediated by formation of superoxide and peroxynitrite.
Taken together, TRPA1 expressed in the endothelium of central arteries is involved in endothelial-dependent vasodilation, whereas TRPA1 expressed in vagal and primary sensory neurons functions in neurogenic vasodilation in peripheral arteries.
5. Pathological and physiological relevance of redox signal-regulated TRPM2 activation
TRPM2 activation induced by H2O2 promotes cell death due to sustained elevation of intracellular Ca2+  and also increases inflammation and tissue injury . Therefore, numerous reports have shown the implications of TRPM2 activity in oxidative stress-induced cell death of many kinds of tissues and cells . Endogenous and exogenous agents that promote ROS production such as TNFα, β-amyloid, and neurotoxin MPTP also cause neurotoxicity in a TRPM2-dependent manner [17, 58–61]. In addition, ischemia/reperfusion (I/R) injury is a major pathological situation involving unregulated ROS production in stroke, brain trauma, cardiac arrest and other disorders and diseases. Ischemia is the restriction of blood flow that diminishes oxygen (hypoxia/anoxia) and glucose supplies to tissues. When blood supply is restored to the affected tissues, secondary effects associated with I/R injury can occur wherein inflammatory agents and ROS that can cause tissue damage are produced. ROS-evoked TRPM2 activity was reported to aggravate tissue damage in the presence of I/R injury [62–64], and this situation can be explained in part by increased neutrophil migration toward affected tissues [63, 65].
On the other hand, more recent reports suggest that TRPM2 could exert protective roles in I/R injury. One group reported that TRPM2 channels are expressed in the sarcolemma and transverse tubules of adult cardiac myocytes [66, 67]. They also showed that TRPM2KO heart is vulnerable to I/R injury and I/R-induced prolongation of action potential duration was enhanced in TRPM2KO myocytes compared with wild type . Proteomic analysis of I/R-affected ventricles from wild-type and TRPM2KO mice revealed that, relative to wild-type heart, mitochondrial respiratory complex dysfunction in TRPM2KO heart is more severe and is associated with altered expression levels of proteins localized in the mitochondrial inner membrane . Under I/R conditions, TRPM2KO myocyte mitochondria showed lower mitochondrial membrane potential, Ca2+ uniporter activity, ATP levels and oxygen consumption as well as higher ROS levels compared to those seen for wild type. These data suggest that Ca2+ supplied by TRPM2 activity can have a protective role by ameliorating mitochondrial dysfunction and diminishing mitochondrial ROS levels in I/R situations. Similar results were recapitulated in intact and TRPM2-depleted SH-SY5Y neuroblastoma cells treated with the ROS-generating chemotherapeutic agent doxorubicin . Interestingly, these diminished parameters of mitochondrial function in TRPM2KO myocytes were also seen under normoxic conditions . Considering these results, TRPM2 may contribute to the maintenance of basal mitochondrial bioenergetics by supplying Ca2+, which has numerous functions in mitochondrial metabolism .
TRPM2 also has roles in inflammation and infection as evidenced by its expression in immunocytes (Table 1). In inflammatory situations, ROS are produced and ROS-evoked TRPM2 activity can aggravate inflammation. At sites of inflammation, phagocytes such as neutrophils and macrophages digest deleterious agents and increase oxygen consumption that enhances NOX-mediated production of ROS, phagocytized agents can then be cleared by ROS. TRPM2 is widely expressed in leukocytes, including lymphocytes, neutrophils, monocytes/macrophages, dendritic cells, microglia and mast cells [19, 25, 71–75]. TRPM2 activation by ROS is reported to worsen inflammation by elevating cytokine release. Indeed, TRPM2 activation in mouse monocytes elevates H2O2-induced release of the neutrophil-attracting chemokine macrophage inflammatory protein-2 (CXCL2) through activation of the Ca2+-dependent tyrosine kinase (Pyk2) and nuclear factor κB (NFκB), and this elevation was attenuated in TRPM2KO monocytes . Supporting these
|The effects of TRPM2 down regulation|
|DSS-induced colitis ||CXCL2↓, neutrophil infiltration↓|
|Survival rate↓, CXCL2→,|
neutrophil infiltration→, iNOS+ monocyte↓
|CLP-induced sepsis ||Survival rate↓, inflammation↑,|
IL-6↑, HMGB↑, bacterial clearance↓
|PA i.t., CLP ||Survival rate↓, bacterial clearance↓|
|LPS i.p. ||Survival rate↓, inflammation↑, CXCL2↑, TNFα↑, IL-6↑, neutrophil|
infiltration↑, ROS production↑
|Stimulus||The effect of TRPM2 down regulation|
|U937 monocyte ||H2O2||CXCL8 (human homolog of CXCL2)↓|
|Peritoneal macrophage ||Zymosan||CXCL2↓, GCSF↓, IL-1α↓, IL-1β↓#,|
|BMDM ||Liposome||Inflammasome activation↓, IL-1β↓,|
TNFα→, ROS production→
|U937 monocyte ||High glucose||Inflammasome activation↓,|
NOX activation↓, IL-1β↓
|BMDM ||PA, SA||Phagosomal acidification↓,|
Nod-like receptor family pyrin domain containing-3 (NLRP3) inflammasomes are activated by several conditions of cellular stress, including microbial products, particulate substances, elevated plasma glucose levels that accompany metabolic disorders, intracellular [K+] reduction and [Ca2+]i increase [77, 78]. NLRP3 inflammasome is a complex of NLRP3, apoptosis-associated speck-like protein (ASC) and caspase-1 to activate caspase-1 that in turn promotes release of pro-inflammatory cytokines, IL-1β and IL-18. TRPM2 is reported to be involved in the inflammasome activation in macrophages/monocytes. Charged liposomes evoke ROS production, caspase-1 activation and IL-1β release from bone-marrow-derived macrophages (BMDM) . Caspase-1 activation and IL-1β release evoked by liposomes are attenuated by TRPM2 deficiency, although mitochondrial ROS production is comparable to that of wild type. Thioredoxin-interacting protein (TXNIP) is known to bind to NLRP3 and participate in ROS-dependent inflammasome activation . TXNIP expression is up-regulated by high glucose and is involved in ROS production induced by high glucose . Treatment of U937 monocytes with high levels of glucose induced TRPM2 up-regulation, ROS production, caspase-1 activation and IL-1β release, which can be attenuated by TRPM2-siRNA or TRPM2 inhibitors . TXNIP up-regulation by high glucose is also inhibited by TRPM2-siRNA. Upon activation of NOX, p47phox, a cytosolic subunit of NOX, translocates to the plasma membrane. Under high glucose conditions, p47phox translocation is increased as is its co-localization with TRPM2. In addition, co-localization of TXNIP and NLRP3 is enhanced by high glucose conditions, which could also be suppressed by TRPM2-siRNA. These data suggest that Ca2+ influx through TRPM2 can contribute to high glucose-induced ROS production as well as inflammasome activation and amplification of TRPM2 activation to further exacerbate oxidative stress in diseases such as type 2 diabetes.
An important role of macrophages, phagocytosis, is regulated by TRPM2 activity. As mentioned above, macrophages phagocytose deleterious agents such as exogenous pathogens and digest them in phagosomes by producing ROS levels that are toxic to the pathogens. This phagocytosis function is known to be enhanced by temperatures that are associated with fever . Phagocytosis induced by toll-like receptor 2 agonist was enhanced by temperature elevation to febrile range in wild-type macrophages which can detect slight temperature change through TRPM2 activity in the presence of low concentration of ROS . On the other hand, TRPM2KO macrophages showed no such temperature-dependent elevation of phagocytosis, suggesting that ROS generated in phagosomes increases TRPM2 sensitivity to body temperature and that TRPM2 activation by temperature increases phagocytosis. Because TRPM2 is highly localized in phagosomal membranes , functional cooperation between TRPM2 and NOX is suggested to increase phagocytosis. As many reports have shown roles for TRPM2 in innate immunity, the physiological roles of TRPM2 have been studied in microbial infection models. In a
Contrary to reports showing that TRPM2 up-regulates inflammation/innate immunity, protective anti-inflammatory roles of TRPM2 were also reported in a LPS-induced lung inflammatory model . After intraperitoneal injection with LPS, TRPM2KO mice had higher mortality with pronounced lung edema than did wild-type mice. Histopathology of tissues from TRPM2KO mice showed increased levels of tissue cytokines including CXCL2, TNFα, IL-6 and myeloperoxidase (MPO) activity, suggesting enhanced neutrophil infiltration in mice lacking TRPM2. ROS release and oxidative DNA damage in TRPM2KO cells are significantly larger than those in wild-type cells, and a TRPM2 inhibitor increased ROS release from wild-type cells that was equivalent to that of TRPM2KO, thus indicating the inhibitory effects of TRPM2 activity. As activity of the electrogenic NOX enzyme is known to be voltage-sensitive , membrane depolarization through TRPM2 is suggested to negatively regulate NOX activity, and the absence of this regulatory mechanism in TRPM2KO mice could enhance ROS production and aggravate inflammation. Nonetheless, the role of TRPM2 in immunity remains controversial, and its activation could depend on the stimulus type, particularly since immunogens can activate multiple cascades (Table 1). In addition, immune reactivity is strongly related to body temperature [83, 91]. Thus, the temperature dependence of TRPM2 must be considered . Outcomes could also be affected depending on the extent of TRPM2 activity, e.g., Ca2+ influx through TRPM2 could be beneficial for many Ca2+-dependent events, whereas large Ca2+ influx could cause Ca2+ overload.
TRPM2 is also known to contribute to the regulation of blood glucose levels (Figure 4) likely because of its functional expression in pancreatic β-cells , which secrete insulin in response to elevated blood glucose. Although the primary pathway of glucose-stimulated insulin secretion is through ATP-sensitive K+ (KATP) channel closure and L-type voltage-gated Ca2+ channel activation to increase [Ca2+]i, many other ion channels, including TRPM2, are reported to be involved in [Ca2+]i-increases to evoke insulin secretion . ROS are produced in response to extracellular signals such as insulin, cytokines, hormones and blood glucose elevation in pancreatic β-cells [94, 95]. In addition, expression of the antioxidant enzyme catalase and glutathione reductase in the pancreas is very low , indicating that the intracellular redox state of β-cells could be affected at a physiological level by the systemic metabolic state. Intracellular Ca2+-oscillations evoked by high glucose concentrations are attenuated in TRPM2KO pancreatic β-cells . In addition, heat-evoked responses in Wt b-cells were enhanced by H2O2 in a dose-dependent manner, however, TRPM2KO cells completely lacked the response . Glucose-stimulated insulin secretion from pancreatic islets was attenuated by TRPM2 deficiency , and the N-acetyl cysteine-sensitive fraction of glucose-stimulated insulin secretion was increased by temperature elevation from 33 °C to 40 °C in wild type, but not TRPM2KO cells, suggesting insulin secretion could be up-regulated by ROS and temperature-dependent TRPM2 activity . TRPM2KO mice show blunted insulin secretion and higher blood glucose levels in oral and intraperitoneal glucose tolerance tests relative to wild-type mice, which highlights TRPM2 function in blood glucose regulation
TRPM2 could also be involved in hormone-regulated insulin secretion. Glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP) are incretin hormones released from the intestine to enhance glucose-stimulated insulin secretion from pancreatic islets . The up-regulation of insulin release by incretin was shown to be TRPM2-dependent [97, 99]. This increase in insulin secretion is mediated by generation of cAMP and activation of exchange protein directly activated by cAMP (EPAC). By inhibiting the same pathway, insulinostatic effects of ghrelin and adrenaline are reportedly achieved [100, 101]. Although the involvement of ROS-evoked TRPM2 activity in such hormone-regulated insulin secretion has not been defined, TRPM2 participation in glucose metabolism seems likely. In contrast, TRPM2-mediated aggravation of inflammation was reported to cause insulin resistance in peripheral tissues . High-fat diet (HFD)-induced obesity, chronic inflammation and insulin resistance with elevated plasma and tissue cytokines are also reported to be related . TRPM2KO mice show resistance to HFD-induced obesity, as well as lower cytokine levels and macrophage infiltration in adipose tissue compared with wild-type mice . Energy expenditure and glucose uptake to muscle and heart are higher in TRPM2KO mice than in wild-type animals, and this increase is accompanied by elevated expression levels of metabolic and mitochondrial genes. These data suggest that, in addition to regulating insulin secretion, TRPM2 could control glucose metabolism in glucose-consuming tissues.
Several TRP channels, particularly TRPA1 and TRPM2, are redox-sensitive and play important roles in both physiological and pathophysiological phenomena that involve redox signals. Therefore, TRPA1 and TRPM2 would be promising targets to develop medicines to treat redox-related diseases.