Potential of Medicinal Use of Essential Oils from Aromatic Plants

2.1. Antibacterial and antifungal activity of essential oils (EO)

The antimicrobial action of essential oils is not yet fully understood, but can be attributed to their permeability to the cell wall of microorganisms due to their diverse chemical and synergistic composition. The hydrophobic characteristic of the essential oils acts in the partition of the lipids of the cellular membrane and the mitochondria, making them more permeable, in this way, the critical ions and molecules (lipids, proteins and nucleic acids) are extravasated, leading them to death. EOs generally have less action on gram-positive bacteria than on gram-negative bacteria due to the interaction of the hydrophobic components of the essential oils and the cell membrane [19, 20, 21].

Different methods are used to evaluate the antibacterial and antifungal properties. The most used are: the method of disc diffusion of Agar, Minimal Inhibition Concentration (MIC), Minimum Bacteria Concentration (MBC) and Minimum Fungicide Concentration (MFC). Since the use of the disc diffusion method in agar is limited by the hydrophobic nature of essential oils and plant extracts that prevents its uniform diffusion through the agar medium, most authors report the results obtained with MIC and MBC [22].

In recent years, different microbial species of medical interest have been tested, from which encouraging results have emerged. Table 1 shows data on the antimicrobial activity of essential oils on fungi and bacteria, also showing the main components of essential oil.

The potential antimicrobial activity of essential oils of the Hedychium coronarium Koen rhizome from different locations in Eastern India was studied in gram-positive, gram-negative bacteria and fungal strains. The study revealed that the essential oils presented more satisfactory effects to the antifungal action than to the antibacterial activity. In addition, the gram-positive bacteria are more sensitive to oil than gram-negative due to the peptidoglycan layer did not selectively act on essential oil compounds. The antimicrobial action of the essential oils was attributed to its constituents in an isolated way, as well as synergistically, additive or antagonistic to each other [23].

Essential oils isolated from Nepeta leucophylla, Nepeta ciliaris, Nepeta clarkei and Calamintha umbrosa showed significant antifungal activity in vitro against phytopathogenic fungi responsible for plant diseases. Essential oils have the potential to be used as a possible biofungicide (as an alternative to synthetic products) that may contribute to an increase in the pre and post harvest storage life of food crops [25].

The good results obtained encourage future research aimed at a possible application of these substances in food, pharmaceutical and cosmetology fields. Table 1 presents the main chemical components of essential oils of several plants with antimicrobial potential.

2.2. Antioxidant activity

The interest in the study of the antioxidant substances of essential oils has become more and more intensified and is now indispensable for the prevention of diverse pathologies [27]. In the literature, it is reported the presence of antioxidant activity in several essential oils [28, 29, 30].

This property acts at different levels in the microorganism protection and plays a key role in some of the biological activities of essential oils, being able to combat the development of oxidative stress that causes damage to health, increasing the risk of diseases such as Alzheimer’s, Parkinson’s and inflammation associated with atherosclerosis and rheumatoid arthritis. Some studies point out that these diseases may be consequences of damages caused by free radicals, besides oxygen and reactive nitrogen species that act as mediators of inflammation as messenger molecules. This shows that essential oils can also act as an anti-inflammatory agent [31, 32, 33].

Essential oils have great potential in the nutrition industry in view of their antioxidant properties, they are use as feed additives for farm animals, for example, and that may be fundamental to the quality of food products from these animals, since essential oils can improve nutritional value, oxidative stability and increase the shelf life of these products such as meats and eggs. In addition, they are often treated as foods to enhance the taste and organoleptic properties, and even has the function of decreasing the process of deterioration of food. The latter is mainly due to its antimicrobial and antioxidants activities [31, 34, 35].

The interest in extracts rich in natural antioxidants has recently increased, especially the antioxidant activity of essential oils. Most of them confirm the assumption that essential oils are promising as natural antioxidants, which can replace synthetic additives such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) that are potentially harmful to human health [36, 37, 38]. In this context, Table 2 presents some more recent studies found in the literature based on the antioxidant activity of essential oils, highlighting its main constituents and antioxidant performance evaluation methods.

2.3. Anticancer activity

Essential oils from aromatic plants have been treated as a product containing anticancer properties because they have the ability to inhibit cell proliferation and decrease the spread of cancer, improving the quality of life of cancer patients and reducing the level of their agony. Mediated therapy with essential oils can be used in combination with conventional therapies in the treatment of cancer (quimioterapia e radioterapia) [44, 45, 46].

According to the World Health Organization [47] cancer is a generic term used for a large group of diseases that can affect any part of the body, is characterized by the growth of abnormal cells beyond their usual limits in the body. Other common terms used are malignant tumors and neoplasms, the latter process or stage of the disease is called metastasis. Cancer is a major public health problem and is considered the second leading cause of death worldwide, accounting for 8.8 million deaths by 2015, where nearly 1 in 6 deaths is caused by cancer. Ref. [48] reported that the American Cancer Society reported in the year 2017 approximately 1,688,780 new cases of cancer and 600,920 deaths from cancer in the United States. According to [49, 50, 51] the most common causes of cancer death are melanoma, leukemia, followed by lung, liver, prostate, breast, cervical, colorectal, and endometrial cancers.

The sharp increase in the number of cancer cases can be attributed to eating habits, since foods contain many chemicals such as preservatives and dyes, making people more susceptible to cancer, which can also be accentuated with the use of tobacco and alcohol, chronic infections, exposure to harmful radiation, or due to change in lifestyle and environmental pollution [45, 52]. Previous studies have reported that oxidative stress increases the onset of different chronic diseases, including cancer. Reactive oxygen species (ROS) are highly unstable compounds that have the ability to attack cells and tissues in the human body, followed by destructive effects that lead to the beginning of cancer [46, 53].

Therefore, there has been a recent increase in the use of natural products such as spices and plants to replace or accompany common treatments for cancer because of their high costs, side effects and the development of resistance of patients against anticancer drugs [44, 52].

Thus, essential oils from different aromatic plants have anticancer potential against mouth, breast, lung, prostate, liver, kidney, colon, bone, ovary, pancreas, uterus and brain cancer and even in leukemia, glioblastoma, melanoma [45, 54]. Thus [52] have shown that essential oil extracted from cloves (Syzygium aromaticum L.) is an ideal natural source as a chemopreventive agent against breast cancerbetulinic acid and other triterpenes, can be indicated as constituents responsible for anticancer properties [55] which determined that the essential oil of eucalyptus (Pulicaria inuloides) presented anticancer activity against breast, liver and colorectal/colon cancer due to the abundant presence of citronellol, pulegol and citronelil acetate.

The myrtle essential oil (Myrtus communis L.) shows anticancer activity against blood cancer (leukemia) due to the presence of 1,8-cineole, linalool, myrtenyl acetate, and myrtenol [56]. However, [46] have shown that orange peel oil (Citrus sinensis) has anticancer properties against colorectal/colon, prostate and lung cancer, withd-limonene being the predominant chemical constituent. Therefore, the results of studies justify the use of essential oils, as a possible alternative medicine in the treatment of cancer.

Essential oils act in the chemoprevention and suppression of cancer, which involve apoptosis, cell cycle retention, antimetastatic and antiangiogenic, increased levels of reactive oxygen and nitrogen species (ROS/RNS), modulation of DNA repair and others that demonstrate their antiproliferative cancer cell activity [53, 57]. In addition, the lipophilic nature of the EOs allows them to cross cell membranes and enter easily within the cell [45, 54], in Table 3 we can observe the anticancer activities of different aromatic plants.

2.4. Antiparasitic activity

Current treatment media control most diseases of protozoan origin mainly through chemotherapy, where synthetic drugs are generally used, but they show several side effects of cytotoxicity in humans. Due to the hydrophobic and bioactivities nature of its components, essential oils (EO) can be considered important sources of development of agents against intracellular pathogens such as protozoa, which cause parasitic diseases [64].

The EO of leaves of Artemisia indica showed antimalarial activity in vitro, being a prophylactic potential of malaria, which is a disease caused by the protozoan of the genus Plasmodium. The oil inhibited at least two recombinant enzymes from the biosynthesis of plasmid fatty acids and showed low cytotoxicity in mammals [65].

Another EO that presents the antimalarial effect is that obtained from Piper aduncum leaves, with camphor (17.1%), viridiflorol (14.5%) and piperitone (23.7%) being the main components found in this oil [66]. The EO of the leaves of Aniba canelilla (HBK) Mez presented a trypanocidal effect, being considered a potential for the natural treatment to trypanosomosis, which is caused by the protozoan Trypanosoma evansi, since it proved its action in vivo. Its antiprotozoal activity is related to the compounds 1-nitro-2-phenylethane (83.68%) and methyleugenol (14.83%), the latter being slightly more active than the first in the treatment of the disease [67].

The EO of the leaves of Tetradenia riparia presented antileishmanial effect in vivo and in vitro, being effective in the fight against the protozoan of the species Leishmania (Leishmania) amazonenses, without showing toxicity to human erythrocytes. The main compound responsible for this therapeutic effect is the 6,7-dehydroroyleanone, which was also tested in isolation and showed a similar effect to the EO [68]. EO from Lippia alba, was investigated in vitro and in vivo assays to evaluate antiparasitic effects and histopathological changes of tambaqui (Colossoma macropomum). Concentrations of 1280 and 2560 mg/L showed 100% efficacy after 20 min of oil exposure in (Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri) [69].

The antiparasitic activity of Lavandula stoechas oil was investigated in Leishmania major, Leishmania tropica and Leishmania infantum. The evaluation of the antileishmanial activity of Lavandula stoechas EO presented a greater effect in comparison to the drug Glucantime. The bioactive compounds present in this oil are: fenchone (31.81%), camphor (29.60%), terpineol (13.14%), menthone (8.96%) and eucalyptol [70].

The anthelmintic activity of Thymus vulgaris L. EO was investigated in in vitro and in vivo tests to evaluate the effect on Haemonchus contortus parasites present in the gastrointestinal system of sheep. Thymol is the major compound corresponding to 50.22% of the oil from the Thymus vulgaris species. Results showed that EO inhibited 96.4% of egg incubation, 90.8% of larval development and 97% of larval mobility [71]. Other essential oils, their chemical constituents and biological antiparasitic activities are shown in Table 4.

2.5. Anti-inflammatory activity

Essential oils have complex mixtures of chemicals that are present in different concentrations, these oils are used in medicine to treat a myriad of diseases because they present potential for anti-inflammatory activity [78, 79].

Inflammation is typically a protective mechanism that can be stimulated by a variety of harmful agents, which may be chemical, physical or biological. Living and vascular tissues respond to stimuli that are considered irritating to the body. These irritations can usually be linked to pain, redness (erythema), heat, tumor (edema), tissue loss or organic function [80, 81].

In recent years the anti-inflammatory potential of essential oils and their chemical position has become the object of study of several researchers in the search for new drugs of natural origin [82, 83, 84], as well as a study of the synergistic anti-inflammatory effect of the chemical constituents of essential oils and synthetic drugs, showing a possible association between clinical remedies with natural products as a pharmacological alternative and avoiding adverse reactions caused by synthetic products [85]. In vivo tests performed on rats confirm the potential of these essential oils as natural products, helping to advance research [86, 87].

The knowledge of the chemical composition and the chemootype of the aromatic plants are important factors in studies of the anti-inflammatory activity, since the concentration of the compounds diverge due to this biological variation, in this way researchers have evaluated both aspects [88, 89]. Evaluating the specific constituents of a particular essential oil may help in understanding the performance of these compounds in the anti-inflammatory action [90]. Table 5 shows the anti-inflammatory potential of different essential oils.