Open access peer-reviewed chapter

Protein Kinase G-Iα Hyperactivation and VASP Phosphorylation in Promoting Ovarian Cancer Cell Migration and Platinum Resistance

By Janica C. Wong and Ronald R. Fiscus

Submitted: May 22nd 2012Reviewed: September 17th 2012Published: February 27th 2013

DOI: 10.5772/53468

Downloaded: 1547

1. Introduction

Platinum-based drugs such as cisplatin (cis-diammine-dichloro-platinum, also commonly known as CDDP) have dominated the drug therapy of ovarian cancer during the past three decades [1]. Cisplatin interacts with DNA to form intrastrand crosslink adducts, and its molecular mechanism involves regulation of p53 and the mitogen-activated protein kinase (MAPK) signaling pathway [2]. The phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway is crucial for regulation of survival and for progression and chemoresistance in ovarian cancer, leading to the development of new chemotherapeutic inhibitors targeting the PI3K/Akt pathway and the downstream serine/threonine protein kinase mTOR. [3]. Inhibition of PI3K pathway signaling using PI3K or mTOR inhibitors has been shown to sensitize ovarian cancer cell lines to the apoptosis-inducing effect of platinum compounds [4, 5]. In addition, activation of the PI3K/Akt/mTOR pathway in ovarian cancer cell lines contributes to cisplatin resistance [6]. The anti-apoptotic, pro-angiogenic effects of PI3K/Akt/mTOR may be mediated, at least in part, through a downstream signaling pathway involving endogenous endothelial-form nitric oxide synthase (eNOS, also called NOS3), and subsequently soluble guanylyl cyclase (sGC) and protein kinase G (PKG). Studies have shown that Akt activates eNOS by phosphorylating human eNOS at Ser1177 (equivalent to bovine eNOS at Ser1179), leading to an increase in nitric oxide (NO) production in endothelial cells [7, 8]. In the cases of vascular endothelial growth factor (VEGF) [9, 10], sphingosine 1-phosphate [11, 12], and estrogen [13, 14], there are vast evidences suggesting PI3K-activation of Akt is responsible for regulating the phosphorylation and activation of eNOS. In bovine aortic endothelial cells, eNOS co-immunoprecipitates with Akt, indicating that the two enzymes associate in vivo, and Akt directly activates eNOS, increasing eNOS activity by 15-20 fold [15]. This signaling pathway has been shown to play an essential role in promoting angiogenesis or tumor vascularization [16]. In a very recent study, microgravity stimulated tube formation and migration in human umbilical vein endothelial cells (HUVEC), and the process was mediated through the PI3K-Akt-eNOS signal pathway [17].

Our early studies of the NO/cyclic GMP (cGMP)/PKG signaling pathway have identified PKG as a key mediator of vasodilation and anti-hypertensive effects induced by NO as well as atrial natriuretic peptide (ANP) [18-21]. Recent studies from our laboratory have shown that the PKG-Iα splice variant of PKG, at basal or moderately elevated activity, plays an important cytoprotective role in preventing spontaneous apoptosis and promoting cell proliferation in many types of mammalian cells, including neural cells [22-27], human ovarian cancer cells [28-30], primary murine vascular smooth muscle cells [31] and murine bone marrow mesenchymal (stromal) stem cells [32]. Evidence from our laboratory suggested that basal activation of PKG-Iα leads to increased attachment of cells to the extracellular matrix and increased cell migration, shown in bone marrow-derived mesenchymal (stromal) stem cells [32]. We have identified certain intracellular proteins that are directly phosphorylated and functionally regulated by PKG-Iα, including 1) the apoptosis-regulating protein BAD [26], 2) vasodilator-stimulated phosphoprotein (VASP) [28, 31, 32], 3) the oncogenic tyrosine kinase c-Src [28, 33] and 4) the transcription factor cAMP responsive element binding protein (CREB) [24, 34], which may contribute to the exaggerated proliferation, enhanced chemoresistance and increased cell migration and invasion in ovarian cancer cells (Figure 1). Our recent studies have shown that cisplatin regulates the endogenous expression of nitric oxide synthases (NOSs) in human ovarian cancer cells, upregulating inducible nitric oxide synthase (iNOS, also called NOS2) expression but dramatically downregulating the expression of eNOS and neural-form nitric oxide synthase (nNOS, also called NOS1), which is involved in determining cisplatin resistance in ovarian cancer cells [30]. Our studies show that the chemoresistance/cytoprotective effects of endogenous eNOS involve hyperactivation of PKG-Iα in the ovarian cancer cells [28].

Studies from our laboratory suggest that PKG-Iα promotes proliferation in ovarian cancer cells, which involves the enhancement of the tyrosine kinase activity of c-Src [28], an oncogenic protein often overexpressed and/or hyperactivated in many types of cancer cells. We showed that PKG-Iα plays a key role in activating c-Src and promoting cell proliferation, using the short interfering RNA (siRNA) or RNA interference (RNAi) technique, to knockdown the expression of PKG-Iα in ovarian cancer cells [28]. We found that epidermal growth factor (EGF)-induced activation of c-Src tyrosine kinase activity causes tyrosine phosphorylation of PKG-Iα, increasing the serine/threonine kinase activity of PKG-Iα and its growth-promoting effects in ovarian cancer cells [28]. Later, we have found that PKG-Iα directly phosphorylates c-Src at Ser17, which enhances the tyrosine kinase activity of c-Src in both in vitro and intact-cell experiments [33]. This novel interaction between PKG-Iα and c-Src causes reciprocal phosphorylation, which means PKG-Iα and c-Src phosphorylate each other, potentially setting up an “oncogenic reinforcement” resulting in exaggerated DNA synthesis and cell proliferation (Figure 1).

Figure 1.

Model of the biological role of PKG-Iα in ovarian cancer cells illustrating the effects of growth factors (e.g. EGF), which stimulates both the PI3K/Akt pathway, enhancing eNOS activity and low-level NO generation and the activation of c-Src. The low, physiological levels of NO activate sGC, elevating cGMP levels that enhance the activation of PKG-Iα. PKG-Iα is further activated (hyperactivated) by the combined effects of cGMP allosteric stimulation and the tyrosine phosphorylation by c-Src. PKG-Iα phosphorylates several downstream proteins, including c-Src, Bad, CREB and VASP, leading to enhanced cell proliferation and cytoprotection, contributing to chemoresistance in ovarian cancer cells and increased cell migration and invasion.

2. Phosphorylation of the vasodilator-stimulated phosphoprotein (VASP) at Ser239 as a useful indicator of endogeneous PKG kinase activity

Vasodilator-stimulated phosphoprotein (VASP) was first described in 1987 as a protein phosphorylated in platelets in response to vasodilators such as sodium nitroprusside, nitroglycerin and various prostaglandins that elevate cAMP and cGMP [35]. VASP belongs to the Ena/VASP family which includes VASP, Mena (mammalian enabled) and EVL (Ena VASP-like). The Ena/VASP family proteins function as anti-capping proteins [36, 37], regulating the actin cytoskeleton dynamics [38-42] and are therefore important for actin-based adhesion [43, 44], migration [45-47] and cell–cell interaction [48-50]. Many studies from others have suggested the involvement of VASP in invasion, angiogenesis and tumorigenesis. In an in vitro model of capillary morphogenesis using human umbilical vein endothelial cells (HUVECs) in three-dimensional collagen gels, the differentiated endothelial cells showed 2 to 3-fold increase in migration with increased VASP mRNA and protein expression [51]. A study on human placenta development showed that VASP may participate in vasculogenesis and endothelial sprouting during placental vasculogenesis, and VASP expression was stimulated by vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) [52]. NIH-3T3 fibroblast deficient in VASP showed loss of contact inhibition, and continued cell division past confluence, while overproduction of VASP by transfection in NIH-3T3 fibroblasts resulted in neoplastic transformation, suggesting a role of VASP in tumorigenesis and/or cancer progression [53]. In human osteocarcinoma specimans, higher VASP expression was associated with metastasis and increased migration, and VASP expression was regulated by Rac1 [54]. In lung adenocarcinomas tissues, VASP expression was increased compared to normal lung tissues, and was significantly increased with more advanced tumor stage [55]. Elevated VASP expression was also reported in human breast cancer tissues [56] and was implicated on invasion and migration in breast cancer cells involving the Rac1 pathway [57]. Moreover, it was shown that in mice lacking VASP, melanoma growth was greatly impaired [58]. In gastric cancer cells, VASP was upregulated by epidermal growth factor (EGF) and promoted migration and invasion. Using microRNA (miRNA) expression profiling of the paired normal/tumor gastric tissues, the same group identified miR-610 as a novel miRNA regulated by EGF that targets VASP in gastric cancer cells [59].

VASP has been reported to be phosphorylated by cAMP-dependent protein kinase (PKA) and cGMP-dependent protein kinase (PKG) [35, 60]. VASP was found to be primarily present as a 46 kDa membrane-associated protein in its dephosphorylated form in platelets, and VASP is converted to an apparent 50 kDa phosphoprotein upon phosphorylation, as observed on Western blot [61, 62]. VASP contains three phosphorylation sites, Ser157, Ser239 and Thr274, all of which can be phosphorylated by either PKA or PKG [63]. Ser157 is the preferred site of phosphorylation for PKA, Ser239 is the preferred site for PKG, and Ser157 was the site responsible for the phosphorylation-induced mobility shift of VASP on Western blots [63]. Because it was well-characterized that VASP at Ser239 is the preferred phosphorylation site for PKG in vitro and in mammalian cells, VASP phosphorylation at Ser239 has been proposed to be a useful indicator of endogenous PKG kinase activity [61, 63, 64]. In fact, VASP at Ser239 was shown to be a functional biomarker of endothelial nitric oxide/cyclic GMP signaling [65], and could be used to indicate defective nitric oxide/cGMP signaling and endothelial dysfunction [66]. In colon cancer cells, VASP Ser239 phosphorylation was used as a biomarker for the action of the anti-cancer drug Exisulind, an inhibitor of type-5 phosphodiesterase (PDE-5) that elevates cGMP and stimulates PKG activation, and that constitutively activated mutants of PKG resulted in direct in vivo phosphorylation of VASP Ser239 [67].

We had shown that the endogenous NO/cGMP signaling pathway in ovarian cancer cells causes a constitutive downregulation of p53 protein expression, which likely contributes to the chemoresistance and exaggerated cell proliferation in these cells [29]. Furthermore, we have previously identified that PKG-Iα is the predominant isoform of PKG in both OV2008 (cisplatin-sensitive, wild-type p53) and A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells as determined by Western blot analysis as well as using the new, ultrasensitive NanoPro100 capillary electrophoresis-based nano-fluidic protein analysis system [28, 68, 69]. Our more recent data now show that the chemoresistance and exaggerated cell proliferation are likely mediated by the constitutive hyperactivation of PKG-Iα (reflected in the high levels of VASP phosphorylation at Ser239) in ovarian cancer cells, and that the PKG-Iα is already activated to approximately 90% of maximal activity, described in our previous book chapter [68]. In our recent study, epidermal growth factor (EGF)-induced activation of Src family kinase (SFK) was found to tyrosine-phosphorylate PKG-Iα increasing its serine/threonine kinase activity in ovarian cancer cells. The EGF-stimulated increase in PKG-Iα kinase activity (indicated by VASP Ser239 phosphorylation) was blocked by both SKI-1 and SU6656 (SFK inhibitors). Using the specific PKG-Iα kinase inhibitor DT-2 and small interfering RNA (siRNA) PKG-Iα gene knockdown, we showed that the inhibition of endogenous PKG-Iα kinase activity reduced VASP Ser239 phosphorylation and DNA synthesis rate in ovarian cancer cells [28]. New data from our laboratory show that the knockdown of PKG-Iα expression inhibits the EGF-stimulated increases in VASP Ser239 phosphorylation and Src/SFK autophosphorylation at the equivalent of Tyr416 (the phosphorylation site for activating the tyrosine kinase activity) in A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells (see Figure 2 below).

Figure 2.

A, EGF (10 ng/mL) elevated VASP Ser239 phosphorylation and Src Tyr416 phosphorylation in A2780cp cells, assessed by Western blot analysis. Gene knockdown of PKG-Iα by PKG-Iα-siRNA partially inhibited the basal VASP phosphorylation and Src/SFK autophosphorylation and completely inhibited the EGF-stimulated increases in VASP phosphorylation and Src/SFK autophosphorylation. The Western blot shown is representative of four experiments. B, Quantification of the relative levels of VASP and Src phosphorylation from Western blot. Bar graphs show mean ± SEM from four independent experiments. *, P< 0.05, compared with no EGF control; #, P<0.05; ##, P<0.01; ###, P < 0.001, compared with negative control.

3. Role of PKG in invasion/migration in A2780cp ovarian cancer cells

The role of NO/cGMP/PKG pathway in invasion/migration in cancer cells is largely unknown. However, a significant number of reports have shown that the NO/cGMP/PKG pathway plays a key role in endothelial cell migration and angiogenesis, involving the downstream activation of the mitogen-activated protein kinase (MAPK) family. It has been shown that NO promotes endothelial cell migration and neovascularization by activating the PI3K/Akt signaling pathway in a PKG-dependent manner [70]. Activation of the NO/cGMP/PKG pathway also promoted endothelial cell angiogenesis and increased extracellular signal regulated kinase 1/2 (ERK1/2) and p38 phosphorylation [71, 72], which were blocked by soluble guanylyl cyclase (sGC) inhibitor, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), or PKG inhibitor DT-3 [73-75]. Moreover, the mitogenic effect of vascular endothelial growth factor (VEGF) on endothelial cells appears to be mediated by endogenous NO (from eNOS) and cGMP, which results in PKG activation and PKG-mediated downstream stimulation of MEK and ERK [76, 77]. Although it has not yet been reported which isoform of PKG is involved in the multiple pro-angiogenesis responses of endothelial cells, our recent studies suggest that endothelial cells express predominantly the PKG-Iα isoform (unpublished observations by J.C. Wong and R.R. Fiscus), which likely mediates the stimulation of downstream growth-promoting and pro-angiogenesis pathways in endothelial cells.

Interestingly, in colon cancer cells, recent studies showed that activation of PKG inhibited cell migration [78], and cGMP-dependent VASP phosphorylation suppressed the number and length of locomotory (filopodia) and invasive (invadopodia) actin-based organelles [79], suggesting a role of VASP Ser239 in invasion and migration. Our studies suggest that the opposite roles of PKG in regulating apoptosis, proliferation and migration reported by others are likely dependent on cell type, growth conditions (presence of different growth factors), as well as the differential expression of PKG-Iα and PKG-Iβ isoforms. The two splice variants of PKG-I, PKG-Iα and PKG-Iβ, are activated by different concentration ranges of NO and are localized to different subcellular locations within cells. Therefore, the two PKG-I isoforms can phosphorylate different sets of downstream target proteins and can mediate completely different biological responses. The very different biological roles of the two PKG-I isoforms are reviewed in further detail elsewhere in another recent book chapter from our laboratory [68]. For example, PKG-Iα (Kact = 0.1 µM by cGMP allosteric activation) is activated at low, physiological levels of NO, whereas PKG-Iβ is activated at higher, pathological levels of NO and requires at least 10-times higher levels of cGMP for activation (Kact = 1 µM) [68, 72, 80, 81].

In our hypothesis, the PKG-Iα and PKG-Iβ isoforms mediate opposite biological effects on cell proliferation and apoptosis, based on observations in two types of cells that express one isoform of PKG-I or the other. Our studies have shown that human ovarian cancer cells express predominantly the PKG-Iα isoform, and that the activation of this kinase by endogenous low-level NO (0.01 – 1 nM), generated by endogenous eNOS and nNOS, would selectively activate the PKG-Iα isoform within ovarian cancer cells from our laboratory, promoting DNA synthesis/cell proliferation and suppressing apoptosis, thus contributing to chemoresistance [28, 30, 68]. Studies in our laboratory, using both normal and malignant cells, including vascular smooth muscle cells, bone marrow-derived mesenchymal (stromal) stem cells and neuroblastoma cells, have suggested that a major role of the low-level-NO/cGMP/PKG-Iα signaling pathway is to protect these cells against the toxic/pro-apoptotic effects of high-level NO, as might occur during inflammation and exposure of cells to pro-inflammatory cytokines [22, 24, 25, 31, 32]. In contrast, based in part on published data from the laboratories of Weinstein and Thompson, it appears that when PKG-Iβ is activated by the higher levels of NO, the growth-inhibitory and pro-apoptotic effects of PKG-Iβ predominate over the growth-stimulatory and anti-apoptotic effects mediated by PKG-Iα. Their laboratories have shown that in colon cancer cells, PKG-Iβ is the predominant PKG-I isoform expressed. Upon activation, PKG-Iβ phosphorylates two downstream target proteins, β-catenin and MEKK1, resulting in inhibition of cell proliferation and induction of apoptosis [78, 82, 83].

As stated above, we have previously determined that PKG-Iα is the predominant isoform in A2780cp ovarian cancer cells [30, 68, 69]. To study whether PKG-Iα plays a role in cell migration/invasion in ovarian cancer cells, we performed experiments using small interfering RNA (siRNA) gene knockdown against PKG-Iα in transwell migration studies. Figure 3 shows that siRNA gene knockdown of PKG-Iα dramatically decreases no EGF as well as EGF-stimulated cell migration (as reflected by the quantity of migrated cells at the bottom of the transwell, stained with crystal violet) in A2780cp cisplatin-resistant ovarian cancer cells. These data confirm the role of endogenous PKG-Iα activity, potentially via VASP Ser239 phosphorylation, in promoting cell migration/invasion in ovarian cancer.

Figure 3.

PKG-Iα siRNA gene knockdown in A2780cp cells decreased both basal and EGF-stimulated cell migration assessed by in vitro cell migration (invasion) assay. Migration of cells was assessed using transwells (Corning) with 8 µM pore polycarbonated inserts, coated with growth factor-reduced matrigel (BD Bioscience). The upper chamber contained 4 x 104 cells and the lower chamber contained 0.6 ml of complete medium with or without EGF. Migration through the membrane was determined after 24 h of incubation at 37°C. Cells remaining on the topside of the transwell membrane were removed using a cotton swab, and cells migrated to bottom were stained with 0.5% crystal violet.

4. Inhibition of the PKG-Iα signaling pathway enhances sensitivity of ovarian cancer cells to cisplatin-induced apoptosis – Potential involvement of cAMP-response-element-binding protein (CREB) and inhibitor of apoptosis proteins (IAPs)

Platinum-based drugs such as cisplatin have dominated the drug therapy of ovarian cancer during the past three decades [1]. Cisplatin interacts with DNA to form intrastrand crosslink adducts, and its molecular mechanism involves regulation of p53 and the mitogen-activated protein kinase (MAPK) signaling pathway [2]. It has been shown that inhibition of ERK1/2 activation with the mitogen-activated protein kinase/ERK kinase 1 (MEK1) inhibitor PD98059 resulted in decreased p53 protein half-life and diminished accumulation of p53 protein during exposure to cisplatin [84]. Our data have shown that human ovarian cancer cells express all of the key components of the NO/cGMP/PKG signaling pathway, including all three isoforms of NOSs, thus providing an endogenous source of NO [30]. Furthermore, ovarian cancer cells continuously produce NO at low physiological levels, activating the heme-dependent soluble guanylyl cyclase (sGC) [29], elevating cGMP levels sufficiently enough to cause continuous high-level activation of PKG [28]. Our data suggested that such basal sGC/cGMP activity regulates p53 expression, and promotes cell survival in part through regulation of caspase-3 [29] (now thought to be mediated by downstream hyperactivation of PKG-Iα).

Cisplatin is also widely employed in chemotherapy on treating solid tumors such as lung cancer. Recently, we showed that, in NCI-H460 and A549 non-small cell lung cancer (NSCLC) cells, PKG-Iα phosphorylates cAMP-response-element-binding protein (CREB) at Ser133 [34]. CREB was first shown to be phosphorylated by PKG in vitro by Colbran et al., which showed that PKG effectively phosphorylates CREB at Ser133, although at a slower rate compared to PKA [85]. Interestingly, NO was shown to regulate the c-fos promoter involving soluble guanylyl cyclase (sGC) and PKG [86] in a CREB-dependent manner [87]. They also showed that transfection of PKG in baby hamster kidney (BHK) cells activated the c-fos promoter [88], which required nuclear translocation of PKG and phosphorylation of CREB at Ser133 by PKG [87, 89, 90]. In our recent study, inhibition of the sGC/PKG-Iα signaling pathway by ODQ (sGC inhibitor), DT-2 (PKG-Iα kinase inhibitor) and PKG-Iα-siRNA gene knockdown showed that PKG-Iα kinase activity is necessary for maintaining higher levels of CREB phosphorylation at Ser133 and the protein expression of certain inhibitor of apoptosis proteins (IAPs), specifically c-IAP1, livin and survivin, as well as the anti-apoptotic Bcl-2 family member Mcl-1, preventing spontaneous apoptosis and promoting colony formation [34]. In the same study, we discovered that DT-2 and cisplatin have a synergistic effect on the induction of apoptosis, with DT-2 dramatically enhancing the pro-apoptotic effects of cisplatin in A549 cells (a NSCLC cell line that requires higher levels of cisplatin to induce apoptosis). We also showed that prior activation of PKG-Iα by 8-bromo-cGMP (8-Br-GMP), a cell-permeable cGMP analog that directly activates PKG [22, 24], has cytoprotective effects against cisplatin. PKG-Iα activity stimulated by 8-Br-cGMP was reflected by increased VASP phosphorylation at Ser239. Pretreatment of A549 cells with 8-Br-cGMP caused significant protection against cisplatin-induced apoptosis, even at higher concentrations of cisplatin. Interestingly, when the same treatments were used on PKG-Iα knockdown cells, the cytoprotective effects of 8-Br-cGMP against cisplatin-induced apoptosis was completely abolished, confirming that the cytoprotection (chemoresistance) was mediated by PKG-Iα [34].

To investigate whether such synergism occurs in ovarian cancer cells, we tested the combined treatment of the specific PKG-Iα kinase inhibitor, DT-2, and cisplatin in the A2780cp cisplatin-resistant ovarian cancer cell line. Our new preliminary data presented in this book chapter (illustrated in Figure 4) verified the synergistic effects of DT-2 and cisplatin. Figure 4 shows the level of apoptosis in A2780cp cells after a 24-hr co-treatment of DT-2 (5 or 10 µM) and cisplatin (2 µM). The Cell Death Detection ELISAPLUS assay (Roche Applied Science), based on quantitative sandwich-enzyme-immunoassay-principle with monoclonal antibodies directed against DNA and histones, were used to quantify apoptotic fragments. DT-2 (5 µM) or cisplatin (2 µM) alone did not cause significant increase in apoptosis. However, combined treatment of DT-2 (5 or 10 µM) and cisplatin (2 µM) significantly (###P<0.001) increased apoptosis, showing a synergistic effect.

Figure 4.

Synergistic effect of DT-2 with cisplatin in A2780cp human ovarian cancer cells. Combined treatment of DT-2 (5 or 10 µM) and cisplatin (2 µM) significantly (###P<0.001) increased apoptosis, compared to cisplatin (2 µM) alone. **P<0.01, ***P<0.001, compared to no DT-2 control. Statistical analysis was performed by one-way ANOVA, followed by Newman-Keuls Multiple Comparison Test using GraphPad (PRISM software). Results were expressed as the mean ± SEM of at least six different samples.

Based on our study of the roles of sGC/PKG-Iα/CREB/IAPs in cisplatin resistant non-small lung cancer cells, we have proposed the anti-apoptotic role of PKG-Iα observed in A2780cp cells is likely mediated through PKG-Iα downstream phosphorylation of CREB at Ser133 and activation of certain IAPs. IAPs have been shown to regulate apoptosis and tumorigenesis [91]. Although how CREB regulates apoptosis through IAPs is largely unknown, it was shown that CREB phosphorylation is a key event in the induction of certain IAPs, c-IAP2 and livin, via multiple protein kinases, PKA, ERK1/2 and p38 MAPK, in colon cancer cells [92, 93]. In ovarian cancer cells, X-linked inhibitor of apoptosis protein (XIAP) has been shown to control ovarian tumor growth and regulate Akt activity and caspase-3 in cisplatin-induced apoptosis [94-96], and the ability of cisplatin to down-regulate XIAP may be an important determinant of chemosensitivity [97]. Down-regulation of XIAP sensitized cells to cisplatin in the presence of wild-type p53, and both XIAP and Akt modulated cisplatin sensitivity individually but that XIAP required Akt for its full function [98]. Inhibition of PI3K/Akt/mTOR signaling has been shown to activate apoptosis and inhibit migration and invasion in ovarian cancer cells [3, 4, 99-104]. Furthermore, inhibition of PI3K pathway signaling using PI3K or mTOR inhibitors has been shown to sensitize ovarian cancer cell lines to induction of apoptosis by platinum compounds [4, 5]. Several recent evidences have suggested that such effects involve the matrix-metalloproteinases (MMPs) [105-107], which are zinc-dependent endopeptidases capable of degradation of extracellular matrix proteins.

5. Overall model of NO/cGMP/PKG-Iα signaling pathway in ovarian cancer

Figure 5 illustrates our overall model showing the involvement of the NO/cGMP/PKG-Iα pathway in promoting cell proliferation and suppressing apoptosis in human ovarian cancer cells, which would contribute to enhanced tumor growth and chemoresistance. Our early studies of the NO/cGMP/PKG pathway have identified PKG as a key mediator of vasodilation and anti-hypertensive effects induced by NO as well as atrial natriuretic peptide (ANP) [18-21]. Recent studies from our laboratory have shown that basal or moderately elevated PKG-Iα activity plays a cytoprotective role in preventing spontaneous apoptosis and promoting cell proliferation in many types of mammalian cells, including neural cells [22-27], human ovarian cancer cells [28-30], primary murine vascular smooth muscle cells [31] and murine bone marrow stromal cells [32]. We found that murine bone marrow-derived mesenchymal (stromal) stem cells endogenously produced ANP and that basal NO/cGMP/PKG-Iα activity and autocrine ANP/cGMP/PKG-Iα activity are necessary for preserving cell survival and promoting cell proliferation and migration in the OP9 bone marrow stromal cell line [32]. Recently, we have identified certain intracellular proteins phosphorylated by PKG-Iα, including BAD [26], vasodilator-stimulated phosphoprotein (VASP) [28, 31, 32], c-Src [28] and cAMP responsive element binding protein (CREB) [34]. We have recently shown that PKG-Iα directly phosphorylates BAD at Ser155, using in vitro experiments, and have further shown that a large part of the Ser155 phosphorylation of BAD within neuroblastoma cells is dependent on endogenous PKG-Iα kinase activity, contributing to decreased caspase-3 activity and inhibition of apoptosis [26].

Figure 5.

Cellular model of the involvement of the NO/cGMP/PKG-Iα signaling pathway in promoting chemoresistance, tumor growth and angiogenesis in ovarian cancer. A special role of VASP phosphorylation at Ser239 may contribute to enhanced ovarian cancer cell migration and invasion.

As illustrated in the model of Figure 5, we have also identified an important role of c-Src/PKG-Iα interaction in promoting DNA synthesis and cell proliferation in human ovarian cancer cells. Previous studies have shown that PKG-I binds Raf-1 and promotes downstream activation of MEK and ERK1/2 in endothelial cells [76]. In ovarian cancer cells, we proposed that PKG-Iα binds to Raf-1 at the internal surface of the plasma membrane, bringing PKG-Iα in close proximity to one of its downstream target proteins c-Src. This leads to downstream activation of the Raf-1/MEK/ERK signaling pathway, promoting cell proliferation. We found that PKG-Iα directly phosphorylates c-Src at Ser17, which enhances the tyrosine kinase activity of c-Src in both in vitro and intact-cell experiments [33]. Our recent studies have shown a clear role of the PKG-Iα-mediated phosphorylation of c-Src at Ser17 in preventing apoptosis and promoting proliferation, attachment and migration in the mesothelioma and NSCLC cells. It is very likely that a similar PKG-Iα catalyzed phosphorylation of c-Src at Ser17 occurs in human ovarian cancer cells, which can explain the dependence of the c-Src activation by EGF on the presence of PKG-Iα [28]. Epidermal growth factor (EGF)-induced activation of c-Src tyrosine kinase activity was found to cause tyrosine phosphorylation of PKG-Iα, increasing the serine/threonine kinase activity of PKG-Iα (indicated by phosphorylation of the PKG substrate VASP at Ser239) and its growth-promoting effects in ovarian cancer cells [28]. In human ovarian cancer cells, the c-Src-mediated tyrosine-phosphorylation of the EGF receptor was found to be highly dependent on PKG-Iα kinase activity [28].

We hypothesized in ovarian cancer cells, as reported in the lung cancer cells in our recent study [34], that PKG-Iα phosphorylated CREB at Ser133, and the cGMP/PKG-Iα signaling pathway maintains the expression of certain IAPs such as c-IAP1, livin and survivin as well as the anti-apoptotic Bcl-2 family member Mcl-1, leading to decreased activity of caspase-3 and promoting cell survival. In ovarian cancer cells where PKG-Iα is hyperactivated, increased downstream phosphorylation of CREB at Ser133 and increased IAPs expression may explain the development of resistance to cisplatin-induced apoptosis. Moreover, PKG-Iα siRNA gene knockdown also decreased both basal and EGF-stimulated cell migration in A2780cp ovarian cancer cells, as shown in Figure 3.

VASP phosphorylation at Ser239 has been shown to be a useful indicator of endogenous PKG kinase activity, both in our recent studies [28, 31, 32] and reports from others [61, 63, 64]. In the current study in this book chapter, we show that siRNA gene knockdown of PKG-Iα expression inhibited EGF-stimulated increases in VASP Ser239 phosphorylation and Src/SFK autophosphorylation in A2780cp (cisplatin-resistant, mutated p53) ovarian cancer cells. Therefore, VASP Ser239 phosphorylation may be a useful biomarker in ovarian cancer cells, and hyperactivation of the unique NO/sGC/PKG-Iα signaling pathway may be a novel therapeutic target for regulation of cancer cell migration/invasion.

Also shown in the model of Figure 5 is the role of endothelial cells, which would provide an additional source of endogenous NO within the growing tumor, potentially contributing to the “angiogenic switch”, i.e. the increased tumor growth that occurs after the invasion of endothelial cells into the tumor. Endothelial cells also play another important role in tumor growth by providing new blood vessels needed for the vascularization and blood perfusion of the growing tumor. In endothelial cells, heat shock protein 90 (HSP90) and Akt activate eNOS involving the formation of a HSP90-Akt-Calmodulin (CaM)-eNOS complex, leading to an increase in NO production [108-111]. Interestingly, HSP90 activation of eNOS can be Ca2+-dependent [112] or Ca2+-independent [109, 113].

6. Future experiments

Our future studies will need to determine: 1) whether PKG-Iα is the only isoform of PKG expressed in other human ovarian cancer cell lines as well as in tumor samples of ovarian cancer patients, 2) the subcellular localization of PKG-Iα (and possibly PKG-Iβ), for example, membrane, nuclear, and/or cytosolic localization, 3) the roles of PKG-Iα, its downstream phosphorylation of CREB at Ser133 (and other transcription factors), expression of the IAPs and anti-apoptotic Bcl-2 family proteins in ovarian cancer cells.

7. Conclusions

The NO/cGMP/PKG-Iα pathway and the downstream phosphorylation of the actin-filament/focal-adhesion-regulating protein VASP at Ser239 appear to promote migration/invasion and the downstream phosphorylation of BAD at ser155, CREB at ser133 and c-Src at ser17 appear to promote DNA synthesis, cell proliferation and platinum resistance in ovarian cancer cells. The unique features of this signaling pathway in ovarian cancer cells may provide a novel therapeutic target for disrupting tumor growth and the metastasis and secondary tumor formation during ovarian cancer progression.

Acknowledgements

Financial. support for the research and the preparation of this book chapter was provided by Internal Funding from Roseman University of Health Sciences (formerly named University of Southern Nevada), Henderson, Nevada, USA, U.S. Department of Defense (DOD) grant W81XWH-07-1-0543 and Start-up Funding from the Nevada Cancer Institute, Las Vegas, Nevada, USA, awarded to Dr. Fiscus.

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Janica C. Wong and Ronald R. Fiscus (February 27th 2013). Protein Kinase G-Iα Hyperactivation and VASP Phosphorylation in Promoting Ovarian Cancer Cell Migration and Platinum Resistance, Ovarian Cancer - A Clinical and Translational Update, Iván Díaz-Padilla, IntechOpen, DOI: 10.5772/53468. Available from:

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