Open access peer-reviewed chapter

Anticancer Effects of Some Medicinal Thai Plants

By Pongtip Sithisarn and Piyanuch Rojsanga

Submitted: October 11th 2016Reviewed: January 27th 2017Published: July 5th 2017

DOI: 10.5772/67648

Downloaded: 1402

Abstract

Ethanolic extracts from thirty Thai edible plants collected from Sa Keao province, Thailand, were screened for in vitro antiproliferative effect on HCT-116 human colon cancer cell line using cell titer 96 aqueous one solution cell proliferation assay. It was found that leaf extract of Crateva adansnii, fruit and leaf extracts of Ardisia elliptica, shoot extract of Colocasia esculenta, leaf extract of Cratoxylum fomosum, and leaf extract of Millettia leucantha exhibited antiproliferative activities. The fruit extract of Ardisia elliptica showed the highest antiproliferative activity. Ethanolic extract of the stems from C. fenestratum and its dichloromethane and aqueous fractions showed antiproliferative activity to human colorectal cancer cells (HCT-116) determined by cell growth assay. Berberine, one of the major alkaloid in the stems of C. fenestratum, also promoted antiproliferative effect. Extracts from the leaves of three Azadirachta species in Thailand, A. indica, A. indica var. siamensis, and A. excelsa, were reported to promote in vitro antioxidant effects determined by various methods. Ten Russula mushroom collected from northeastern part of Thailand were tested for in vitro antioxidant activities using photochemiluminescence assay for both lipid-soluble and water-soluble antioxidant capacities. R. medullata extract exhibited the highest antioxidant effects in both lipid-soluble and water-soluble models.

Keywords

  • anticancer
  • Coscinium fenestratum
  • berberine
  • Azadirachta
  • Russula

1. Introduction

Cancer cells uncontrollably divide to form masses of tissue, which are called tumors. Tumors can grow and interfere with the functions of many bodily systems including the digestive, nervous, and cardiovascular systems. Cancer has been reported to be the first in the rank of causes of the death in the Thai population. Liver, colon, and lung cancers are the most prevalent cancers in Thai males, while breast, cervical, and colon cancers are the most prevalent cancers in Thai females [1].

The development of cancer or carcinogenesis occurs through a multistep process involving the mutation, selection of cells with a progressive increasing capacity for proliferation, survival, invasion, and metastasis [2]. The first step in the process, tumor initiation, relates to the genetic alteration leading to the changes in normal cells. Then, in the promotion or development stage, the cells abnormally proliferate leading to the outgrowth of a population of clonally derived tumor cells [2]. This stage can be stimulated by carcinogens, which are a group of substances such as tobacco, asbestos, arsenic, radiation such as gamma and X-rays, sun light, polycyclic hydrocarbons, nitrosamines, and aflatoxins: these substances do not directly cause cancers but promote or aid the development of cancers [2, 3]. After that, tumor progression continues as additional mutations occur within the cells of the tumor population to further advantage the cancer cells, such as more rapid growth, which will allow them to become dominant within the late tumor population. The process is called clonal selection, since a new clone of tumor cells evolves on the basis of its increased growth rate or other properties such as survival, invasion, or metastasis. Clonal selection continues throughout tumor development, so tumors continuously become more rapid-growing and increasingly malignant [2].

Advertisement

2. Cancer therapy

The modern treatments for cancers mainly are surgery, radiation, and chemotherapy. However, most of chemotherapeutic drugs are not specific to only cancer cells, but also cause damage to normal cells, especially bone marrow, mucous glands, mucous membranes, hair, and nails and can lead to the suppression of the immune system [3]. The success of chemotherapy depends on the number of cancer cells, the proliferation rate, the duration of the drug administration, and the therapeutic interval. To avoid drug resistance, polychemotherapy is always used instead of monochemothearpy [3]. The anticancer drugs can also cause some other side effects including nausea, vomiting, agranulocytosis, inhibition of spermatogenesis and ovulation, alopecia, inflammation of mucous membranes, and terratogenesis [3].

Some compounds separated from natural products are now being developed as modern medicines for the treatments of cancers including paclitaxel, catharanthus alkaloids, and derivatives of podophyllotoxin.

Paclitaxel was separated from the bark of Taxus brevifoliaNutt. (Pacific Yew), which is a tree in Taxaceae. Paclitaxel will bind with b-tubulin and stimulate the aggregation of a tubulin subunit to become a nonphysiological microtubule composed of 12 proto-filaments, which cause the inhibition of cell cycles in mitosis and interphase (G2-phase) and lead to cell apoptosis. This compound is normally used in an injection formulation as the adjuvant chemotherapy for the treatments of ovarian, breast, and bronchial cancers [3].

Some alkaloids are separated from the leaves of Catharanthus roseus(L.) G. Don., such as vincristine and vinblastine. Vincristine is used for the treatment of lymphatic leukemia, neuroblastoma, and Wilms tumor, while vinblastine is used to treat lymphogranuloma (Morbus Hodgin), lymphosarcoma, testicular carcinoma, and chorionic carcinoma [3].

Podophyllotoxin was separated from the rhizome of Podophyllum peltatumL. or American mandrake. Two derivatives of podophyllotoxin, etoposide and teniposide, are now being developed and used as anticancer drugs. Etoposide is used for the treatment of bronchial cancer, testicular carcinoma, and chorionic carcinoma, while tenoposide is used to treat brain or bladder cancers [3]. The chemical structures of some anticancer compounds from natural products are shown in Figure 1.

Figure 1.

Chemical structures of some anticancer compounds from natural products. A = paclitaxel, B = vinblastine, C = vincristine, D = etoposide, E = teniposide.

3. Anticancer effects of medicinal plants and natural products

Natural products from plants, animals, marine sources, and minerals have been used for the treatments of ailments and diseases for a long time. In Thai traditional medicine, the word “cancer” could refer to the symptom of chronic wound, abscess, emaciation, and weak [4]. Active phytochemicals in plants can be classified into two main groups of primary metabolites, which are the compounds necessary for plant growth and development such as carbohydrates, proteins, and fats. Another group is secondary metabolites, which promote the defense mechanisms or support the lives of the plants; they include polyphenolic compounds, flavonoids, terpenoids, and alkaloids [5]. Ethanolic extracts from thirty Thai local edible plants collected from Wang Nam Yen district, Sa Keao province, Thailand were screened for the in vitroanti-proliferative effect on HCT-116 human colon cancer cell lines using a cell titer 96 aqueous one solution cell proliferation assay. It was found that six ethanolic plant extracts, including a leaf extract of Crateva adansnii, fruit and leaf extracts of Ardisia elliptica, a shoot extract of Colocasia esculenta, a leaf extract of Cratoxylum fomosum, and a leaf extract of Millettia leucanthaexhibited antiproliferative activities on the HCT-116 cell line. The fruit extract of Ardisia ellipticashowed the highest antiproliferative activities with an IC50 value of 5.12 ± 0.54 μg/ml [6]. The mechanisms of the action of medicinal plants for anticancer effects have been reported as following [4]:

3.1. Inhibition of cell division in the cancer cell cycle

Alpha-mangostin from mangosteen (Garcinia mangostana) fruit rind promoted inhibitory effects to breast cancer cell line (MDA-MB-231) by inhibition of cell division in G1 and S phases [7]. Methanol extract of Morus albaL. leaves inhibited liver cancer cell line Hep G2 by inhibition of cell division in G2/M phase [8]. Cucurbitacin B, a triterpenoid from Trichosanthes cucumerinaL., also inhibited breast cancer cell division in G2/M phase [9].

3.2. Induction of cancer cell apoptosis

This mechanism includes some minor mechanisms which stimulate anticancer genes, induction of caspase enzymes, induction of free radical formation, inhibition or induction of enzymes relating to histone protein, and the formation of spingosine or ceramide [4]. Dehydrocostus lactone from the root of Saussurea lappainduced the apoptosis of liver cancer cells Hep G2 and PLC/PRF/5 via p53 protein [10]. Water extract of the seed from Sapindus rarakCandolle. induced lung cancer cells A549 apoptosis through the induction of the caspase enzyme [11], while methanol extract of Derris scandensBenth. induced apoptosis of colon cancer cells SE480 by increased caspase-3 activity and down-regulated Bcl-2 and up-regulated Bax protein of SW480 cells; it also significantly induced cell necrosis determined by the release of LDH [12]. Alpha-mangostin separated from the fruit rind of mangosteen also upregulated Bax and down-regulated Bcl-2 proteins in rat liver tissue [13]. Methanol extract from stem bark of Myristica fragransHoutt. promoted the apoptosis of lymphoblast Jurkat by controlling the SIRT1 gene [14]. G1 b, a glycospingolipid from Murdannia loriformis(Hassk.) R.S.Rao & Kammathy, inhibited breast, lung, colon, and liver cell lines [15].

3.3. Immune stimulation

Methanol extract from the leaves of Moringa oleiferaLam. exhibited immune stimulation effect both cell-mediated immunity and humoral immunity by induction of neutrophile production and stimulation of macrophages in animals damaged by the toxicity of anticancer drugs [16].

In Thai traditional medicine, there are some medicinal formulas compose of several plants in different ratios. These formulas are traditionally used for a long time usually for the treatments of cancers in patient with the late stage cancers, patients who cannot improve after treatment with chemotherapy, radiation or surgery, patients with cancers in several organs or patients with incurrent diseases [4]. The sources of anticancer herbal formulas usually come from local traditional doctors or priests in the temples (in Thai, temple is called as “Wat”), with the normal method of preparation being the decoction of plant materials with water [4]. A herbal remedy from Wat Tha-it (Tha-it temple), Ang Thong province, Thailand, composed of several plant materials including Gelonium multiflorumA. Juss., Erycibe elliptilimbaMerrill & Chun, Balanophora abbreviateBlume, Smilax chinaL., Smilax glabraWall. ex Roxb., and Millingtonia hortensisLinn. was reported to significantly promote synergistic effects on doxorubicin in the treatment of A549 cancer cells by the inhibition of cell divisions in the G2/M phase [4, 17]. Another herbal remedy is from a Thai herbal nursing home, Wat Khampramong, Sakon Nakhon province comprises of several plant materials such as Rhinacanthus nasutus(L.) Kurz, Acanthus ebrateatusWall., Smilax glabraWall. ex Roxb., Artemisia annuaL., Angelica sinensis(Oliv.) Diels, Salacia chinensisL., and Orthosiphon aristatusMiq [18]. This herbal remedy can inhibit the growth of some cancer cell lines such as breast adenocarcinoma MDA-MB 231, synovial sarcoma SW982, hepatocellular carcinoma HepG2, cervical adenocarcinoma HeLa, and lung carcinoma A549 [18].

4. Some potential Thai medicinal plants with anticancer effects

4.1. Coscinium fenestratum(Gaertn.) Colebr

NAG-1 or nonsteroidal anti-inflammatory drug (NSAID)-activated gene was identified in COX-negative cells by PCR-based subtractive hybridization from an NSAID-induced library as a divergent member of the TGF-β superfamily [19]. The overexpression of NAG-1 in cancer cells results in growth arrest and an increase in apoptosis, suggesting that NAG-1 has antitumorigenic activity [20]. NAG-1 expression is also upregulated by a number of dietary compounds, medicinal plants, and anticancer drugs [2125]. Coscinium fenestratumis one of the medicinal plants that promoted antiproliferative effects on colon cancer cell lines with mechanisms related to NAG-1 [20].

Coscinium fenestratum(Gaertn.) Colebr. is a large climber with yellow wood and sap, known in the Thai language as Hamm or Khamin khruea. The genus Cosciniumbelongs to the tribe Coscinieae of the family Menispermaceae. This genus comprises two species, which are Coscinium blumeanumMiers. and C. fenestratum(Gaertn.) Colebr. Both of them are stout woody climbers growing in the tropical rain forest regions of Asia [26]. Cosciniumspecies are characterized by the axillary flowers, extra-axillary or cauliflorous in racemiform, or peduncled subumbellate aggregate, of 20–50 cm in length. The inflorescences are axillary or cauliflorous with 6–12 florets. Male flowers are sessile or with pedicels, up to 1 mm. Sepals are broadly elliptic to obovate with the inner 3–6 spreading, yellow, and 1.5–2 mm long. Stamens are 6 with 1 mm long. The Sepals of female flower are as in male flowers. Staminodes are 6 and claviform with 1 mm long. Drupes are subglobose, tomentellous, brown to orange or yellowish, 2.8–3 cm diameter. Pericarp is drying woody. Seeds are whitish and subglobose with the enveloping condyle. The leaves are subpeltate or ovate, large, hard-coriaceous, palmately nerved, reticulate, and densely hairy beneath [26]. Physical characteristic of the Coscinium fenestratumstem (cross section) is shown in Figure 2.

Figure 2.

Physical characteristic ofCoscinium fenestratumstem purchased from Nongkhai province, Thailand (cross section ×1).

The stem decoction and maceration extracts of Coscinium fenestratumhave been traditionally used in the Northeastern part of Thailand for the treatment of various diseases such as cancer, diabetes mellitus, and arthritis [27]. The ethanolic extract of the stems from C. fenestratumand its dichloromethane and aqueous fractions showed antiproliferative activity on human colorectal cancer cells (HCT-116) determined by a cell growth assay. Berberine, one of the major alkaloids in the stems of C. fenestratum, also promoted an antiproliferative effect [20]. The mechanisms of action of the extracts from C. fenestratumwere reported as the activation of proapoptotic proteins and pparγ [20]. It was also reported that berberine facilitated the apoptosis of cancer cells, and the molecular targets for its activity are NAG-1 and AFT3 [24]. The chemical structure of Berberine is shown in Figure 3.

Figure 3.

Chemical structure of Berberine.

4.2. Azadirachtaplants

Oxidative stress is considered to be of some importance for many ailments and pathologies; including cardiovascular diseases, cancers, rheumatoid arthritis, and Alzheimer’s disease [28]. Polyphenolic compounds have been reported to have important anticancer and chemo-preventive effects [29]. Phenolic acids such as gallic acid, ellagic acid, and ferulic acid induce apoptosis in cancer cells, activated caspase, prevented cancer formation, and suppress the angiogenesis of cancer [2932]. Flavonoids such as quercertin and kaempferol also promote apoptosis, inhibit oncogenes, and generated cell cycle arrest [29, 3335].

Suttajit et al. [36] studied the antioxidant activities of extracts from many Thai medicinal plants using a ABTS-metmyoglobin assay and reported some plants with high antioxidant activities; including Uncaria gambierRoxb., Piper betleLinn., Camellia sinensis(L.) Kuntze., Azadirachta indicaA. Juss. var. siamensisValeton., Curcuma zedoariaRoxb., Syzygium aromaticum(L.) Merr. & Perry and Tamarindus indicaLinn. When focusing on Thai medicinal plants, the Siamese neem tree (Azadirachta indicaA. Juss. var. siamensisValeton.) is an interesting plant that showed high antioxidant activity in the screening test [36, 37]. Moreover, there are reports about its antioxidant potential based on the antioxidant content as the butylated hydroxyanisole (BHA) equivalent of Thai indigenous vegetable extracts. From this report, the Siamese neem tree leaf extract appeared to be a high potency antioxidant, containing more than 100 mg BHA equivalent in 100 g fresh weight.

Azadirachtaplants comprise of three different plant species; Azadirachta indicaA. Juss or A. indicaA. Juss var. indica(neem), Azadirachta indicaA. Juss. var. siamensisValeton (Siamese neem tree), and Azadirachta excela(Jack) Jacobs. (marrango tree). The Siamese neem tree leaves are wider, longer, and thicker than the leaves of neem, while the marrango tree has the widest, longest, and thickest leaves. The margin of the leaflet of Siamese neem tree is crenate to entire, while the margin of neem is serrate and that of marrango tree is entire to undulate. The colors of the leaflet blade of the Siamese neem tree, neem, and marrango tree are green, light green, and dark shiny green, respectively [38, 39]. The physical characteristics of Siamese neem tree, neem, and marrango tree leaves are shown in Figure 4.

Figure 4.

Physical characteristics ofAzadirachtaplants; A = Siamese neem tree (Azadirachta indicavar.siamensis), B = neem (Azadirachta indica), C = marrango tree (Azadirachta excela).

The leaves and flowers of Siamese neem tree and neem have been traditionally used as element tonics and antipyretic and gastric secretion stimulating agents, while the stem bark of all Azadirachtaplants is used to treat amoebic dysentery and diarrhea [40, 41]. There also reports suggesting that polysaccharides and limonoids found in neem bark, leaves, and seed oil reduce tumors and cancers and showed effectiveness against lymphocytic leukemia [4244]. Moreover, the young leaves and flowers of the Siamese neem tree are popularly consumed as vegetables [39].

For the antioxidant effect, Azadirachtaplants were reported to promote in vitroactivities tested by various methods. Extracts from the leaves of A. indica, A. indicavar. siamensis, and A. excelsawere reported to promote in vitroantioxidant effects determined by a DPPH scavenging assay, Fremy’s salt assay, ESR detection of POBN spin adducts, and an oxygen consumption assay [45, 46]. The leaf’s aqueous and flower ethanol extracts from the Siamese neem tree provide antioxidant activity on lipid peroxidation formation induced by UV-irradiation of a Chago K-1 bronchogenic cell culture at a concentration of 100 μg/ml determined by the thiobarbituric acid reactive substances (TBARS) method [47].

Cloning and expression analysis of genes involving flavonoid biosynthesis showed that Siamese neem tree leaves total RNA contained nucleotide sequences related to enzymes F3′H, FLS, DFR, and F3′5′H, which could be responsible for the biosynthesis of the antioxidant flavonoids [48]. Some flavonoids that were separated from Siamese neem tree and neem leaves and flowers are kaempferol, myricetin, quercetin, and rutin [39, 4951]. The chemical structures of some flavonoids found in Azadirachtaplants are shown in Figure 5.

Figure 5.

Chemical structures of some flavonoids found inAzadirachtaplants. A = kaempferol, B = myricetin, C = quercetin, D = rutin.

4.3. Russulamushrooms

It is well established that many compounds separated from mushrooms can be used as immuno-modulators or as biological response modifiers [52]. Several mushroom species in Basidiomycetes have been reported to possess anti-tumor activity [53, 54].

Many phytochemical compounds have been reported in various mushrooms, and they can be classified into two main groups: high molecular weight compounds such as beta-glucan and other polysaccharides [55] and low molecular weight compounds including polyphenolics, flavonoids, and terpenoids [52]. Polyphenolics such as caffeic acid, chlorogenic acid, ferulic acid, and gallic acid and flavonoids such as myricetin and catechin were found in Agaricusbisporus, Boletus edulis, Calocybe gambosa, and Cantharellus cibarius[56]. Triterpeniods were found in Agaricus bisporus, Ganoderma lucidum, and Russula lepida. Moreover, aristolane sesquiterpenoids were also found in Russula lepida[57]. Polysaccharides were found in Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum, and Phellinus linteus[58]. Some polysaccharides such as beta-glucan are reported to promote immunomodulatory effects via CR3, the leukocytemembrane receptor for β-glucans [59]. The mechanisms of the action of the mushrooms to promote anticancer effects have been reported as NF-κB inhibitors, protein kinase inhibitors, protein and DNA alkylating agents, modulators of G1/S and G2/M phases, inhibitors of MAPK protein kinase signaling pathways, aromatase and sulfatase inhibitors, matrix metalloproteinases inhibitors, cyclooxygenase inhibitors, DNA topoisomerases, and DNA polymerase inhibitors and anti-angiogenic substances [52].

A previous study reported the presence of 1147 mushroom species in the Northeast part of Thailand. They are composed of 647 consumed mushroom species, 222 trade mushroom species, and 400 poisonous mushroom species [60]. Thirty-seven species of these mushrooms are used in traditional medicine [60]. However, there are still some mushrooms in Thailand, especially in the Northeastern part of the country, that have never been studied for their biological properties and phytochemical compounds.

The Russulamushroom’s shape resembles an umbrella. There have a clear cap and stem, with the gills underneath the cap. The cap is thin and has an underlying radius arranged around the center. The mushroom has no ring and no latex in the cap. The mushroom is fresh, soft, fragile, and perishable [61]. There are around 750 worldwide species of Russula[62, 63]. The distribution of the Russulaspecies shows that they are present in several countries, including the United States of America, Sweden, France, Norway, Madagascar, Italy, Belgium, Taiwan, China, Japan, and Thailand [64]. In Thailand, Russulamushrooms have been found in 17 provinces in the Northeastern region of Thailand [65]. Numerous Russulamushrooms have been consumed as food such as R. monspeliensis, R. virescens, R. alboareolata, R. medullata, and R. helios[65, 66]. Various Russulamushrooms have been traditionally used for the treatments of various diseases such as R. cyanoanthaand R. nobilis, which are used for the treatment of fever; R. luteotacta, which is used for wound healing; and R. delicaand R. parazurea, which are used for the treatment of gastritis and high blood pressure, while R. acrifoliais used for treatments of skin cancer [36]. Moreover, some Russulamushrooms have also been traditionally used for tonic purposes such as R. cyanoxantha, R. nobilis, R. delica, R. parazurea, R. acrifolia, and R. luteotacta[67]. In addition, Russula luteotactahas been used as a sleep promoting agent [67]. Physical characteristics of some Russulamushrooms found in Thailand are shown in Figure 6.

Figure 6.

Physical characteristics of some Russula mushrooms found in Thailand; A =Russula crustosaPeck, B =Russula delicaFries, C =Russula monspeliensisSarnari, D =Russula velenovskyiMelzer & Zvára, E =Russula virescens(Schaeff) Fries, F =Russula alboareolataHongo.

Ten Russulamushroom collected from northeastern part of Thailand: R. crustosa, R. delica, R. monspeliensis, R. velenovskyi, R. virescens, R. lepida, R. alboareolata, R. paludosa, R. medullata, and R. helioswere tested for their in vitroantioxidant activities using a photochemiluminescence assay for both lipid-soluble and water-soluble antioxidant capacities. R. medullataextract exhibited the highest antioxidant effects in both lipid-soluble and water-soluble models with antioxidant capacities of 1.1658 nmol of trolox equivalence and 1.323 nmol of ascorbic acid equivalence, respectively [68].

Some chemical constituents have been reported from Russulamushrooms including phenolic acids such as ρ-hydroxy-benzoic acid, chlorogenic acid, ferulic acid, caffeic acid, protocatechuic acid, and coumaric acid and flavonoids such as quercetin, chrysin, and catechin [6971]. Some terpeniods were also found in Russulamushrooms including aristolane and marasmane [57, 72]. The chemical structures of the constituents found in Russulamushrooms are shown in Figure 7.

Figure 7.

Chemical structures of some flavonoids found inRussulamushrooms. A = ferulic acid, B = chrysin, C = aristolane.

5. Conclusion

Natural products have been main sources of drug discoveries including the development of active compounds or formulas for the treatment of cancers. Even though it has become difficult to discover or synthesize new active components, with the knowledge and intelligence regarding traditional medicine, there are still several ethnomedical herbal formulas and regional plants that could be studied and developed for further medicinal utilizations. Herbal remedies from Wat Tha-it and Wat Khampramong, Thailand, are examples of the efforts to develop anticancer therapies from traditional knowledge. Both remedies can inhibit the growth of various cancer cell lines. The stem extract and active compound, Berberine from the Thai medicinal plant Coscinium fenestratum, significantly promoted anti-proliferative activity on human colorectal cancer cells with the mechanism of action via NAG-1 and AFT3. Plants in the genus Azadirachtahave been traditionally used as a tonic. They promote significant antioxidant activities, which could support the body’s systems and prevent oxidative stress, which is one of the causes of carcinogenesis. Russulais the local mushroom species in the Northeastern part of Thailand. They promote significant antioxidant effects in both lipid-soluble and water-soluble models. These plants and natural products have the potential to be sources of anticancer compounds or active extracts for the treatments of cancer. However, standardization and quality control of the extract or active compounds should be performed before studying the toxicity, in vivobiological activity tests, and further clinical studies in the future.

Advertisement

Acknowledgments

The authors acknowledge the Thailand Institute of Scientific and Technological Research for the support in supplying the photos of Russulamushrooms. The authors would like to thank Dr. Prapaipat Klungsupya for her valuable guidance and support about photochemiluminescence assays. The authors also thank Ms. Charinan Jaengklang for her assistance in the Russulamushrooms work.

© 2017 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to cite and reference

Link to this chapter Copy to clipboard

Cite this chapter Copy to clipboard

Pongtip Sithisarn and Piyanuch Rojsanga (July 5th 2017). Anticancer Effects of Some Medicinal Thai Plants, Natural Products and Cancer Drug Discovery, Farid A. Badria, IntechOpen, DOI: 10.5772/67648. Available from:

chapter statistics

1402total chapter downloads

More statistics for editors and authors

Login to your personal dashboard for more detailed statistics on your publications.

Access personal reporting

Related Content

This Book

Next chapter

Phytocompounds Targeting Cancer Angiogenesis Using the Chorioallantoic Membrane Assay

By Stefana Avram, Roxana Ghiulai, Ioana Zinuca Pavel, Marius Mioc, Roxana Babuta, Mirela Voicu, Dorina Coricovac, Corina Danciu, Cristina Dehelean and Codruta Soica

Related Book

First chapter

Evaluation of Resveratrol Supplementation on Laboratory Animals, Cats, Pigs, Horses, Dogs, Cattle, and Birds

By Mary U. Ememe, Anthony K.B. Sackey and Joseph O. Ayo

We are IntechOpen, the world's leading publisher of Open Access books. Built by scientists, for scientists. Our readership spans scientists, professors, researchers, librarians, and students, as well as business professionals. We share our knowledge and peer-reveiwed research papers with libraries, scientific and engineering societies, and also work with corporate R&D departments and government entities.

More About Us