Open access peer-reviewed Edited Volume

Modulating Gene Expression

Abridging the RNAi and CRISPR-Cas9 Technologies

Edited by Aditi Singh

Independent scientist


RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.

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Modulating Gene ExpressionAbridging the RNAi and CRISPR-Cas9 TechnologiesEdited by Aditi Singh

Published: May 29th 2019

DOI: 10.5772/intechopen.75144

ISBN: 978-1-83880-697-2

Print ISBN: 978-1-83880-696-5

eBook (PDF) ISBN: 978-1-83880-698-9

Copyright year: 2019

Books open for chapter submissions

6867 Total Chapter Downloads

4 Crossref Citations

2 Web of Science Citations

14 Dimensions Citations


Open access peer-reviewed

1. Introductory Chapter: Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies

By Aditi Singh


Open access peer-reviewed

2. CRISPR-ERA for Switching Off (Onco) Genes

By Ignacio García-Tuñon, Elena Vuelta, Sandra Pérez-Ramos, Jesús M Hernández-Rivas, Lucía Méndez, María Herrero and Manuel Sanchez-Martin


Open access peer-reviewed

3. Gene Silencing Agents in Breast Cancer

By Amal Qattan


Open access peer-reviewed

4. Nontransformative Strategies for RNAi in Crop Protection

By Deise Cagliari, Ericmar Avila dos Santos, Naymã Dias, Guy Smagghe and Moises Zotti


Open access peer-reviewed

5. Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

By Lingwen Zeng, Omar Mukama, Xuewen Lu, Shilin Cao and Donghai Lin


Open access peer-reviewed

6. MultiSite Gateway Technology Is Useful for Donor DNA Plasmid Construction in CRISPR/Cas9-Mediated Knock-In System

By Takeshi Yasuda and Katsushi Tajima


Open access peer-reviewed

7. Machine Learning and Rule Mining Techniques in the Study of Gene Inactivation and RNA Interference

By Saurav Mallik, Ujjwal Maulik, Namrata Tomar, Tapas Bhadra, Anirban Mukhopadhyay and Ayan Mukherji


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

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