Open access peer-reviewed Edited Volume

Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications

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Academic Editor

Craig Atwood
Craig Atwood

University of Wisconsin–Madison,
United States of America

Published26 April 2011

Doi10.5772/2149

ISBN978-953-307-197-8

eBook (PDF) ISBN978-953-51-6432-6

Copyright year2011

Number of pages504

Pluripotent stem cells have the potential to revolutionise medicine, providing treatment options for a wide range of diseases and conditions that currently lack therapies or cures. This book describes methodological advances in the culture and manipulation of embryonic stem cells that will serve to bring this promise to practice.

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Table of Contents

Open access  chapters

1. Embryonic Stem Cells: from Blastocyst to in vitro Differentiation

By Genesia Manganelli, Annalisa Fico and Stefania Filosa

3,586
2. Mouse Embryonic Stem Cells Basics from a Fertilized Zygote to These Promising Pluripotent Stem Cells

By Carol Fagundez, Mónica Loresi, Stella Delcourt and Pablo Argibay

2,565
3. Effective Derivation and Manipulation of Mouse Embryonic Stem Cells

By Peng Zhang, Xinglong Wu, Pengbo Wang and Xiangyun Li

5,104
4. Artificial Acellular Feeder Layer: An Advanced Engineered Extracellular Matrix for Stem Cell Culture

By Amranul Haque, Masato Nagaoka, Xiao-Shan Yue, Stephen A. Duncan and Toshihiro Akaike

1,724
1,925
6. Surface Engineering to Control Embryonic Stem Cell Fate

By Shohreh Mashayekhan and Jun-ichi Miyazaki

2,369
8. Topographically and Chemically Modified Surfaces for Expansion or Differentiation of Embryonic Stem Cells

By Lotte D’Andrea Markert, Jette Lovmand, Mogens Duch and Finn Skou Pedersen

1,915
1
9. Directed Differentation of Human Embryonic Stem Cells in Combination of Biomaterials

By Huihui Wang, Xiaojing Xu, Rong Ye and Huanxiang Zhang

2,367
11. Fourier Transform Infrared Microspectroscopy as a Tool for Embryonic Stem Cell Studies

By Diletta Ami, Paolo Mereghetti, Antonino Natalello and Silvia M. Doglia

2,120
2
14. Use of Transposon-Transposase Systems for Stable Genetic Modification of Embryonic Stem Cells

By Tamás I. Orbán, Ágota Apáti, Zsuzsanna Izsvák, Zoltán Ivics and Balázs Sarkadi

1,710
3,426
1
17. Application of Magnet-based Nanofection in Embryonic Stem Cell Research

By Ssang-Goo Cho, Sachin Honguntikar and Hyun Joo Lee

2,217
20. Tracking the Structure of Protein Interaction Network via Multiple Genetic Perturbations on Mouse Embryonic Stem Cells — Implementation of the Entropy Maximization Principle

By Lei Mao, Rossella De Cegli, Mario Lauria, Grit Nebrich, Jean Maurice Delabar, Yann Herault, Gilda Cobellis and Joachim Klose

1,530
2,073
2
4,242
1
1,891

IMPACT OF THIS BOOK AND ITS CHAPTERS

64,056 Total Chapter Downloads

1,938 Total Chapter Views

16 Crossref Citations

28 Web of Science Citations

37 Dimensions Citations

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