Open access peer-reviewed Edited Volume

Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications

Edited by Craig Atwood

University of Wisconsin–Madison, United States of America

Pluripotent stem cells have the potential to revolutionise medicine, providing treatment options for a wide range of diseases and conditions that currently lack therapies or cures. This book describes methodological advances in the culture and manipulation of embryonic stem cells that will serve to bring this promise to practice.

Order hardcopy
IntechOpen
Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cel...Edited by Craig Atwood

Published: April 26th 2011

DOI: 10.5772/2149

ISBN: 978-953-307-197-8

Copyright year: 2011

Books open for chapter submissions

48737 Total Chapter Downloads

9 Crossref Citations

17 Web of Science Citations

23 Dimensions Citations

chaptersDownloads

Open access peer-reviewed

1. Embryonic Stem Cells: from Blastocyst to in vitro Differentiation

By Genesia Manganelli, Annalisa Fico and Stefania Filosa

2754

Open access peer-reviewed

2. Mouse Embryonic Stem Cells Basics from a Fertilized Zygote to These Promising Pluripotent Stem Cells

By Carol Fagundez, Mónica Loresi, Stella Delcourt and Pablo Argibay

2060

Open access peer-reviewed

3. Effective Derivation and Manipulation of Mouse Embryonic Stem Cells

By Peng Zhang, Xinglong Wu, Pengbo Wang and Xiangyun Li

4155

Open access peer-reviewed

4. Artificial Acellular Feeder Layer: An Advanced Engineered Extracellular Matrix for Stem Cell Culture

By Amranul Haque, Masato Nagaoka, Xiao-Shan Yue, Stephen A. Duncan and Toshihiro Akaike

1258

Open access peer-reviewed

5. Biomaterials for In Vitro Expansion of Embryonic Stem Cells

By Makoto Sakuragi and Yoshihiro Ito

1378

Open access peer-reviewed

6. Surface Engineering to Control Embryonic Stem Cell Fate

By Shohreh Mashayekhan and Jun-ichi Miyazaki

1747

Open access peer-reviewed

7. Preservation of Embryonic Stem Cells

By Xiaoming He

1909

Open access peer-reviewed

8. Topographically and Chemically Modified Surfaces for Expansion or Differentiation of Embryonic Stem Cells

By Lotte D’Andrea Markert, Jette Lovmand, Mogens Duch and Finn Skou Pedersen

1297

Open access peer-reviewed

9. Directed Differentation of Human Embryonic Stem Cells in Combination of Biomaterials

By Huihui Wang, Xiaojing Xu, Rong Ye and Huanxiang Zhang

1815

Open access peer-reviewed

10. Non-invasive Imaging of Human Embryonic Stem Cells Derived Endothelial Cells Using Reporter Genes

By Yizhou Zheng, Deling Kong and Zongjin Li

1196

Open access peer-reviewed

11. Fourier Transform Infrared Microspectroscopy as a Tool for Embryonic Stem Cell Studies

By Diletta Ami, Paolo Mereghetti, Antonino Natalello and Silvia M. Doglia

1523

Open access peer-reviewed

12. Reprogrammed Parthenogenetic ES Cells - New Choice for Regenerative Medicine

By Takuro Horii and Izuho Hatada

1233

Open access peer-reviewed

13. Reprogramming Somatic Cells by Fusion with Embryonic Stem Cells: Present Status and Prospects in Regenerative Medicine

By Jinnuo Han and Kuldip S. Sidhu

3919

Open access peer-reviewed

14. Use of Transposon-Transposase Systems for Stable Genetic Modification of Embryonic Stem Cells

By Tamás I. Orbán, Ágota Apáti, Zsuzsanna Izsvák, Zoltán Ivics and Balázs Sarkadi

990

Open access peer-reviewed

15. Gene Transduction Approaches in Human Embryonic Stem Cells

By David Brafman and Karl Willert

2933

Open access peer-reviewed

16. Pluripotent Stem Cells Induced from Testicular Tissue of a Man with Klinefelter Syndrome (47, XXY) by Four Transcription Factors (OCT4, SOX2, KLF4, and C-MYC)

By Hideyuki Kobayashi

1100

Open access peer-reviewed

17. Application of Magnet-based Nanofection in Embryonic Stem Cell Research

By Ssang-Goo Cho, Sachin Honguntikar and Hyun Joo Lee

1577

Open access peer-reviewed

18. Chromosome Engineering in Mouse Embryonic Stem Cells: Addition and Elimination of Targeted Chromosomes

By Masako Tada

2279

Open access peer-reviewed

19. Microarray Analysis of Undifferentiated and Differentiated Human Pluripotent Stem Cells

By Jane Synnergren and Peter Sartipy

1630

Open access peer-reviewed

20. Tracking the Structure of Protein Interaction Network via Multiple Genetic Perturbations on Mouse Embryonic Stem Cells — Implementation of the Entropy Maximization Principle

By Lei Mao, Rossella De Cegli, Mario Lauria, Grit Nebrich, Jean Maurice Delabar, Yann Herault, Gilda Cobellis and Joachim Klose

1106

Open access peer-reviewed

21. Establishment of Embryonic Stem Cells and Generation of Genetically Modified Rats

By Masaki Kawamata and Takahiro Ochiya

1718

Open access peer-reviewed

22. Rat Embryonic Stem Cells: Establishment and Their Use for Transgenesis

By Masumi Hirabayashi and Shinichi Hochi

1482

Open access peer-reviewed

23. Assessment of Embryotoxicity and Teratogenicity by the Embryonic Stem Cell Test

By Kazuaki Nakamura, Shinji Kusakawa and Akito Tanoue

3728

Open access peer-reviewed

24. Application of Embryonic Stem Cells as a Novel Tool in Drug Screening

By Gi Jin Kim

1540

Open access peer-reviewed

25. Human Pluripotent Stem Cell-Derived Neuronal Networks:Their Electrical Functionality and Usability for Modelling and Toxicology

By Riikka Äänismaa, Laura Ylä-Outinen, Jarno E. Mikkonen and Susanna Narkilahti

1342

Open access peer-reviewed

26. A Novel Strategy for Drug Discovery and Development by Analyzing the Behavior of ES Cells Cultured on TOSHI (Tissue/Organ Sections for Histopathology)–Substrata

By Toshiaki Takezawa

1068

Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

Order a hardcopy of the Edited Volume

Free shipping with DHL Express

Hardcover (ex. VAT)£205

Order now

Residents of European Union countries need to add a Book Value-Added Tax of 5%. Institutions and companies, registered as VAT taxable entities in their own EU member state, will not pay VAT by providing IntechOpen with their VAT registration number. This is made possible by the EU reverse charge method.

Special discount for IntechOpen contributors

All IntechOpen contributors are offered special discounts starting at 40% OFF available through your personal dashboard

Login and purchase